CN106212252A

CN106212252A - A kind of vertical culture vessel

Info

Publication number: CN106212252A
Application number: CN201610818610.1A
Authority: CN
Inventors: 谢海燕; 庞永奇; 晁代印
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-09-13
Filing date: 2016-09-13
Publication date: 2016-12-14

Abstract

The present invention relates to a kind of vertical culture vessel, including: container body, active plugboard, perforated plate, lid, it is characterised in that: described container body and lid fit in a set of, and global shape is vertical vertical structure.Owing to this invention takes above technical scheme, so having the advantage that one is owing to the vertical culture vessel of the present invention have employed vertical vertical structure, experimental subject vertical-growth as naturalness can be made；Two is the plate owing to there being energy activity in the middle of the vertical culture vessel of the present invention, can insert flexibly or take out plate, meet different experiments needs；Three is owing to container body outer surface is the most with a scale, facilitates the visual observations contrast longitudinal growth length of root and the cross growth state of root system and stem and leaf, facilitates and homogeneous puts experiment material such as seed etc.；Four is owing to there being filter membrane on lid, the internal oxygen supply that can obtain abundance of container, the beneficially growth of plant, and does not have condensation vapor inside container；Five is that the vertical culture vessel of the present invention can be with low cost controlled with mass production, easily accepts for user, can be that vast scientific research scientific worker brings practical help.

Description

A kind of vertical culture vessel

Technical field

The present invention relates to a kind of experimental apparatus, a kind of container cultivated for plant or mushroom.

Background technology

Modern plants research and fungus (especially edible fungi) research are required to relate to aseptic or without miscellaneous bacteria bar Cultivate under part.In the initial stage cultivated, it is the most all to carry out in culture dish.Culture dish is many laboratory, hospital, The instrument that the occasion such as school, factory floor is conventional.Conventional culture dish is glass or the circle of plastic production or square hollow vessel, point For the end and lid two parts, overall in flat, height is 10～20mm, and diameter or width are from 35mm to 100mm.Cultivate plant Or during fungus, it is necessary first to pouring a certain amount of culture medium at the bottom of culture dish into, after culture medium cooled and solidified, by seed or strain Or other outer implant material is positioned over media surface or inside, then close the lid good seal, is placed in manually operated environment Middle cultivation.Modern much research needs to observe and measure root system, stem and leaf or mycelia, the upgrowth situation of sporophore, conventional culture dish Owing to space more than shaped flat, culture medium and culture medium is the least, root system and stem and leaf or fungal mycelia and sporophore are raw Long space is very limited, and root system tends not to penetrate completely in culture medium but is partially exposed to media surface, Stem and leaf typically can not stretch simultaneously, but bending lodges and grows, and readily accesses culture medium and directly absorbs nutrition and cause generation Thank to interference.Somewhat length is accomplished by transferring in high culture bottle a little louder, and not only complex operation increases workload, increases and pollutes probability, And growth conditions is difficult to keep coherent consistent, transfer operation also results in certain injury to object of study itself.If it is it addition, many Individual sample kind compares research in same culture dish, then sample room is easy to tied up in knots, be unfavorable for observe measure and Later stage samples.The steam condensed on culture dish lid, can affect the growth of experimental subject.

Summary of the invention

For above-mentioned existing problems, the present invention provides one to be mainly used in plant aseptic culture, simultaneously can be used for fungus The vertical culture vessel cultivated, this culture vessel avoids the shortcoming of conventional culture dish, at least following points advantage: one, makes Plant can be in sufficient space under laboratory aseptic condition, and root system is downwards, stem and leaf is upward with the shape of access expansion as far as possible State grows, it is achieved real vertical cultivation, root is completely into culture medium, root environment stable homogeneous, and blade grows up, and keeps away Contact-free culture medium absorbs nutrition and causes Nutrition and Metabolism to disturb；Two, owing to having increased the height that can record culture medium, make cultivation Base can be layered and record, can simulating realistic soil, need to form the different nutrition of gradient, moisture and other emulation external world according to research Environment, this point is that conventional culture dish does not realizes；Three, middle plate can be inserted and can take out, by culture medium media environment during insertion Being divided into former and later two parts, root growth, in both sides, may be used to study the conduction of root cap long range signals；Four, culture vessel vertically and Horizontal direction is the most with a scale, and the convenient longitudinal growth length observing range estimation and contrast root of vertical scale, horizontal scale facilitates homogeneous Put experiment material such as seed etc., and facilitate visual observations contrast root system and the cross growth state of stem and leaf；Five, container cover The filter membrane of band certain pore size on son, can make filtrated air pass through with microorganisms such as filter bacteria, makes to have inside container sufficiently Oxygen supply；Six, present configuration is ingenious and simple, makes material and technical maturity, can make mould, it is achieved metaplasia in batches Produce, with low cost, thus can provide for the researcher of the aspects such as vast botany and mycology and conscientiously help, the present inventor is Produce the sample of maturation.

In order to achieve the above object, the present invention takes techniques below scheme, and a kind of vertical culture vessel, including container master Body, lid, active plugboard, perforated plate two pieces, it is characterised in that: 1, described container body is vertical vertical structure, vertical direction Highly general at more than 5cm, generally height is about 10cm, hence it is evident that more than conventional culture dish, horizontal direction width typically 2～ 5cm, length typically 8～20cm, the overall flat in vertical direction under usual ratio；2, outer, the upper end band of container body Prominent step, the width that step highlights is rear with on lid lid concordant；3, container body medium position is internal slotted, may be inserted into work Dynamic plate and perforated plate；Active plugboard and perforated plate aspect ratio container body height are slightly lower, and width is joined with container body inside dimension Set, is advisable just snapping in the draw-in groove in container body；There is prominent handle the middle part of active plugboard, and handle height is not higher than Height after on lid lid；4, container body outer surface the most with a scale；5, lid surface is fixed with filter Film, usual aperture is 0.22 micron or 0.45 micron, and if desired for Anaerobic culturel, then lid is airtight, does not paste filter membrane, Ke Yigen Select flexibly according to needs.

Owing to present invention employs above technical scheme, so having the advantage that one is due to the vertical cultivation of the present invention Container have employed vertical vertical structure, is different from generally horizontal flat culture dish, therefore can make experimental subject picture nature shape The such vertical-growth of state；Two is the plate owing to there being energy activity in the middle of the vertical culture vessel of the present invention, makes culture medium can be divided into two Part, both can do the control experiment of different object simultaneously, it is also possible to allow the mycelia of the root system of same plant or fungus toward both sides Culture medium grows, and carries out long range signals conduction studies；Three is owing to container body outer surface the most all bands are carved Degree, vertical scale facilitates ready visual contrast to observe the longitudinal growth length of root, and horizontal scale facilitates homogeneous puts experiment material such as Seed etc., and facilitate visual observations contrast root system and the cross growth state of stem and leaf；Four is owing to having filter membrane, container on lid The internal oxygen supply filtering out the microorganisms such as antibacterial that can obtain abundance, the beneficially growth of plant, there will not be solidifying simultaneously Bearing water vapour, such as Anaerobic culturel to be carried out, then lid is airtight, does not paste filter membrane；Five is that the vertical culture vessel of the present invention can be by mould Tool realizes quantifying to produce, and raw material can use the multiple plastic raw materials being readily available, such as polyethylene, and polypropylene, polrvinyl chloride etc., With low cost controlled, easily accept for user, can be that vast scientific research scientific worker brings practical help, the present inventor is also Produce the sample of maturation.

Accompanying drawing explanation

Fig. 1 is present configuration schematic diagram

1 is container body, and 2 highlight step for container body upper end, and 3 is the internal draw-in groove of container, and 4 is active plugboard, and 5 is band Orifice plate, 6 is lid, and 7 is filter membrane

Detailed description of the invention

The present invention will be further described below in conjunction with the accompanying drawings.

Embodiment one

Such as Fig. 1, a kind of vertical culture vessel, including 1 container body, 2 container body upper ends highlight step, inside 3 containers Draw-in groove, 4 active plugboards, 5 perforated plates, 6 lids, 7 filter membranes.It is internal that described 3 (the internal draw-in grooves of container) are positioned at 1 (container body), card Well width just inserts 4 (active plugboard) one piece and 5 (perforated plates) two pieces, and 5 (perforated plates) insert in 4 (active plugboard) both sides；Institute State 6 (lids) and cover 1 (container body), and stand on 2 (container body upper end highlights step), and the outer rim of 6 (lids) Concordant with the outer of 2 (container body upper end highlights step)；The upper surface middle part of described 6 (lids) hollows out, and sticks aperture and is 7 (filter membranes) of 0.22 μm.

It is a feature of the present invention that: one is culture vessel generally vertically vertical structure to have the culture medium of recording of abundance Space and upper space, the Root growing space of existing abundance, also there is the trunk stem and leaf growing space of abundance, experiment can be made right As vertical-growth as naturalness；Two is that container body is internal slotted, can insert flexibly or take out plate, meets different real Test needs；Three is that container body outer surface is the most with a scale, facilitates the longitudinal growth of visual observations contrast root The cross growth state of length and root system and stem and leaf, facilitates and homogeneous puts experiment material such as seed etc.；Four is band filter on lid Film, can make the internal non-condensing steam of container, and guarantee the aseptic oxygen supply having abundance inside container.The additionally each parts of the present invention Production cost can be largely reduced easy to utilize with mass production.

In addition to the implementation, the present invention can also have other detailed description of the invention.All employing equivalents or equivalence become Change the technical scheme of formation, all fall within the protection domain of application claims.

Claims

1. a vertical culture vessel, including container body, active plugboard, perforated plate, lid, it is characterised in that: described container Main body and lid fit in a set of, overall in vertical vertical structure.

The vertical culture vessel of one the most according to claim 1, it is characterised in that: described container body is internal slotted, its The most supporting active plugboard of groove and perforated plate.

The vertical culture vessel of one the most according to claim 1, it is characterised in that: described container body outer upper ends band is dashed forward Going out step, its step outer is concordant with container cover outer rim.

The vertical culture vessel of one the most according to claim 1, it is characterised in that: described container body outer surface has quarter Degree.

The vertical culture vessel of one the most according to claim 1, it is characterised in that: band filter membrane on described lid.