CN106212001B - A kind of cuttage breeding method of Ilex kudincha C. J. Tseng seedling - Google Patents
A kind of cuttage breeding method of Ilex kudincha C. J. Tseng seedling Download PDFInfo
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- CN106212001B CN106212001B CN201610614780.8A CN201610614780A CN106212001B CN 106212001 B CN106212001 B CN 106212001B CN 201610614780 A CN201610614780 A CN 201610614780A CN 106212001 B CN106212001 B CN 106212001B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The present invention relates to agricultural technology field, a kind of cuttage breeding method of Ilex kudincha C. J. Tseng seedling is disclosed.Methods described processes cuttings in the methyl α-naphthyl acetate that pH value is 6.0 7.0, then cuttage and seedling culture, whne in cuttings axillary bud germinating and root it is long to more than 5cm when transplant, the Nutrition Soil of transplanting is yellow mud, river sand, coconut palm chaff and cow dung and adds the Nutrition Soil of mixing stacking curing after composite fertilizer, when cuttings seedling up to 20 25cm long, Ilex kudincha C. J. Tseng seedling is obtained.The present invention is controlled emphatically in terms of the pH value of growth hormone and the Nutrition Soil two of transplanting hardening, and the aspect such as preparation, the selection of hormone, cuttage link, the seedbed management with cuttings has carried out Promethean process combination, there is provided a kind of effective cottage method, it is not only simple to operate, and growing-seedling period is short, rooting rate and transplanting survival rate are high, and the seedling of acquisition has stronger growing power, compensate for the defect of existing cutting propagation technique.
Description
Technical field
The present invention relates to agricultural technology field, and in particular to a kind of cuttage breeding method of Ilex kudincha C. J. Tseng seedling.
Background technology
Ilex kudincha C. J. Tseng (Ilex kudingcha C.J.Tseng) be also Ilex Latifolia Thunb, is Aquifoliaceae Holly.It is bitter
Fourth tea Chinese ilex is the characteristic integration of drinking and medicinal herbs beverage plant for enjoying great prestige great reputation, has long history of drinking in China, is received for a long time
The extensive concern of people is arrived, more than ten countries and regions have been found a good sale in product prepared by Ilex kudincha C. J. Tseng tender shoots.Currently, bitter leaves
Tea Chinese ilex is distributed mainly on Hainan whole area, Yingde, Guangdong, Zhaoqing and Dabu, Guang Xinan in about 100,000 mu of the cultivated area in the whole nation
Peaceful periphery, big new, day etc., Longzhou, and Yunnan some areas etc..
The old leaf of Ilex kudincha C. J. Tseng and tender shoots can tea medicine it is dual-purpose, do not have local flavor after steeping stain with boiling water, it is first sweet after hardship and return
Taste is long long, its product can drink for a long time it is without any side effects, can cardiac stimulant be healthy and strong, especially be depressured fat-reducing and it is clearing heat and detoxicating and extremely
Consumers in general favor.Research is confirmed, set containing ursolic acid, aromatic oil, kudinchagenin, α-perfume in Ilex kudincha C. J. Tseng blade
Various function ingredients such as fat, cupreol, chlorogenic acid.These effective efficiency compositions have analgesic, antibacterial, spasmolysis, step-down, heat-clearing
Fall fire, slake thirst and help produce saliva, detoxicating, relieving inflammation, cardiac stimulant diuresis, radioresistance, various medicinal efficacies and the health-care effect such as anti-cancer and cancer-preventing.Through poison
Reason experiment and people for a long time drink history identity, Ilex kudincha C. J. Tseng product can substantially reduce T-CHOL (Tch), glycerine
The content of three esters (TG) and low-density lipoprotein (LDL), improves blood viscosity, and arteriosclerosis, coronary heart disease, throat are swollen
Bitterly, chronic pharyngitis also has obvious curative effects.
In recent years, with the fast development of bitter leaves tea industry, the factor of industry sustainable development is influenceed also gradually to manifest, its
Middle most important factor is that Ilex kudincha C. J. Tseng seedling quality is uneven and degeneration is serious.At present, the Ilex Latifolia Thunb planted in production
Chinese ilex seedling is typically the direct clip branch from Wild plant and carries out cuttage and seedling culture, then again through excessive for cutting propagation.Cause
This, the upper Ilex kudincha C. J. Tseng seedling used of production plantation is the sufficiently complex population mixture of a genetic background, the agronomy of plant
Proterties is widely different with quality trait, causes the yield and quality of later stage bitter leaves tea product and cannot ensure.
Ilex kudincha C. J. Tseng is woody magaphanerophytes, and its branch degree of lignification is higher, thus in the prior art cuttage root-taking compared with
Difficulty, and the rooting rate of seedling cutting propagation is relatively low with transplanting survival rate.Accordingly, it would be desirable to provide a kind of effective as early as possible
Ilex kudincha C. J. Tseng seedling cutting propagation technology, is that excellent Ilex kudincha C. J. Tseng seedling spread in cultivation production establishes base
Plinth, solves the problems, such as that Ilex kudincha C. J. Tseng seedling breeding technology is short of, and is that the scale and standardization production of Ilex Latifolia Thunb lay the foundation,
It is expected to alleviate that Ilex kudincha C. J. Tseng seedling quality is uneven and situation of variety deterioration, starts bitter leaves tea industry sustainable development
New frame, to realize that industrial upgrading and hot-zone increasing peasant income provide technical support.
The content of the invention
In view of this, it is an object of the invention to provide a kind of cuttage breeding method of Ilex kudincha C. J. Tseng seedling so that institute
State that cuttage breeding method operation is relatively simple, growing-seedling period is short, and rooting rate is high, transplanting survival rate is high, and the seedling of acquisition has more
Strong growing power.
To achieve these goals, the present invention provides following technical scheme：
A kind of cuttage breeding method of Ilex kudincha C. J. Tseng seedling, including：
Step 1, preparation cuttings；
Step 2, cuttings is immersed in pH value be 6.0-7.0 naphthalene acid solution in process；
Step 3, through methyl α-naphthyl acetate process after cuttings on seedbed cuttage and seedling culture；
Step 4, whne axillary bud germinating in cuttings and root it is long to more than 5cm when transplant hardening, the Nutrition Soil for transplanting hardening is
30% yellow mud, 30% river sand, 30% coconut palm chaff and 10% cow dung simultaneously to be added mix after 3% composite fertilizer of gross weight and stack curing 6
Nutrition Soil more than month, when cuttings seedling up to 20-25cm long, obtains Ilex kudincha C. J. Tseng seedling.
The present invention is more difficult for Ilex kudincha C. J. Tseng cuttage root-taking in the prior art, and the rooting rate of seedling cutting propagation with
The relatively low defect of transplanting survival rate, by control growth hormone pH value and transplanting Nutrition Soil component come solve problem,
Especially by this operation measure of the pH value of control growth hormone, not such as growth hormone kind in existing cuttage technique
Class, concentration and processing mode are given concern like that, and the present invention promotes Ilex kudincha C. J. Tseng seedling by limiting suitable pH
With rooting rate and transplanting survival rate higher.
Wherein, cuttings do not use base portion degree of lignification high or top You Nen positions branch.Preferably, step 1 is：
Chosen from the Ilex kudincha C. J. Tseng maternal plant for growing fine and give birth to the branch of semi-lignified as cutting then and be made slotting
Fringe, the cuttings retains the axillary bud of more than 2, and retains 3-4 piece blades.It is highly preferred that the cuttings retains the armpit of more than 2
Bud, and retain the blade that 3-4 pieces subtract 1/3 leaf area.
In cuttage breeding method of the present invention, the cuttings may be selected to be length 15-20cm, diameter 0.6-1.0cm, cuttings
Upper cut at 2.0cm above axillary bud, lower cut at 5-10cm below axillary bud, the neat crack-free of cuttings otch.
Preferably, the pH value of the growth hormone naphthalene acid solution is 6.5, the naphthalene acid solution concentration is 300mg/
L。
Preferably, step 3 is：
Cuttings is inserted on seedbed, seedbed is sprayed using antiseptic before cuttage, cuttings depth is the 1/5 to 1/ of cuttings overall length
4, insert ear density do not overlapped as standard with blade face, after cuttage is complete immediately by seedbed pour it is permeable once；
The plastic, transparent film shed of 50cm high is set up after cuttage on seedbed and double-deck shade net caping is set at top,
Nursery bed disinfection sterilization is sprayed using antiseptic weekly, holding seedbed humidity is periodically watered more than 80%, and control temperature in shed
Between 28-30 DEG C, cuttings nursery sprays foliar fertilizer to degree after 50 days.Seedbed therein is optional in the specific embodiment of the invention
Select the seedbed with river sand as matrix.
In the transplanting stage of the present invention, can continue to be placed on by the transplantation of seedlings of cuttings kind to being equipped with the bag film of Nutrition Soil
Grown 1-2 weeks (such as in plastic sheeting shed) in original cuttage and seedling culture environment, when cuttings seedling up to 20-25cm long, obtained
Ilex kudincha C. J. Tseng seedling.
The Ilex kudincha C. J. Tseng seedling obtained using cuttage breeding method of the present invention, grows callus, 50 after 40 days
It is visible after it to take root, average root 8.1cm long after 60 days, up to 86.3%, survival rate is up to 80.7% after transplanting for rooting rate.And make
Be the mating system of contrast, its callus grows the time and rootage duration is later than the inventive method, and rooting rate with
And also substantially it is not so good as cuttage breeding method of the invention in the survival rate after transplanting.
Meanwhile, in the contrast test of different growth hormone, concentration, pH value and Nutrition Soil composition, only in this hair
It is bright limit under conditions of, Ilex kudincha C. J. Tseng seedling can possess more significant rooting rate high, transplanting survival rate and life
Ability long.
From above technical scheme, the growth characteristics of present invention foundation Ilex kudincha C. J. Tseng, emphatically from the pH of growth hormone
Two aspects of Nutrition Soil of value and transplanting hardening are controlled, and selection and preparation, the selection and processing of hormone, skewer with cuttings
The aspects such as slotting ring section, seedbed management have carried out Promethean process combination, there is provided a kind of effective seedling cutting propagation
Method, it is not only simple to operate, and also growing-seedling period is short, and rooting rate is high, transplanting survival rate is high, has after the cuttage kind transplantation of seedlings of acquisition
There is stronger growing power, compensate for the defect of existing Ilex kudincha C. J. Tseng seedling artificial breeding.
Specific embodiment
The invention discloses a kind of cuttage breeding method of Ilex kudincha C. J. Tseng seedling, those skilled in the art can use for reference this
Literary content, is suitably modified technological parameter realization.In particular, all similar replacements and change are to art technology
It is it will be apparent that they are considered as being included in the present invention for personnel.The method of the invention has passed through preferably implementation
Example is described, related personnel substantially can not departing from present invention, spirit and scope to method described herein and
Realize and apply the technology of the present invention using being modified or suitably changing with combining.
Just a kind of cuttage breeding method of Ilex kudincha C. J. Tseng seedling provided by the present invention is described further below.
Embodiment 1：Cuttage breeding method of the present invention
The branch of semi-lignified is given birth on clip Ilex kudincha C. J. Tseng maternal plant then as cutting, cutting is cut into appropriate specification
Cuttings, cutting length about 15-20cm, diameter about 0.6-1.0cm.Each cuttings retains 3-4 piece blades, and every blade subtracts 1/3
Area and retain petiole.Each cuttings containing the axillary bud (2-4 individual) of more than 2, cuttings upper cut at 2.0cm above bud, under
Otch at 5-10cm below bud, the neat crack-free of otch of cuttings.Cuttings does not use base portion degree of lignification higher or top
The branch at the tender position of children.
The cuttings made first cleans 5min in clear water, is a wisp by about 30 cuttings bundles, then will bundle agglomerating
Cuttings be immersed in the naphthalene acid solution of 300mg/L and process more than 8 hours, the acid-base value of naphthalene acid solution need to adjust in advance to
PH6.5 or so, cuttings root is rinsed after having processed with clear water.
Cuttings is inserted on the seedbed with river sand as matrix, seedbed, cuttings depth are sprayed using carbendazim solution before cuttage
Degree is with the 1/5 to 1/4 of cuttings overall length, and the cuttings depth of shorter and smaller cuttings is preferably deep unsuitable shallow, inserts ear density with blade face not
Overlap be degree, after cuttage is complete immediately by seedbed pour it is permeable once.
The plastic sheeting shed of 50cm high is taken on seedbed and double-deck shade net caping, holding of periodically watering are set at top
Seedbed humidity more than 80%, and regarding feelings it is aeration-cooling control arch temperature of shed between 28-30 DEG C.
Cuttings nursery sprays a small amount of foliar fertilizer after 50 days, after 60 days whne axillary bud germinate and root it is long to more than 5cm when can move
Plant in nutrition bag film, Nutrition Soil is 30% yellow mud, 30% river sand, 30% coconut palm chaff and 10% cow dung and adds gross weight
Mixed after 3% composite fertilizer and stack curing more than 6 months.
The cuttings seedling of pack is continued to be placed on and is grown in plastic sheeting shed about 1-2 weeks, it is wet in the holding shed that periodically waters
Degree treats cuttings seedling up to 20- long regarding the aeration-cooling control arch temperature of shed of feelings more than 80% between 28-30 DEG C
During 25cm, the Ilex kudincha C. J. Tseng seedling is obtained.
In the present embodiment, cuttings grows callus after 40 days in cuttage and seedling culture, visible after 50 days to take root, and is put down after 60 days
Equal root 8.1cm long, up to 86.3%, survival rate is up to 80.7% after being transferred to the outer hardening of shed for rooting rate.
Embodiment 2：With the branch at the branch of base portion degree of lignification higher part position and top You Nen positions as cuttings
Cottage method
In addition to cuttings selects difference, remaining step and environment are with embodiment 1.
Result of the test：Cuttings grows callus after 50 days in cuttage and seedling culture, visible after 60 days to take root, average root after 70 days
4.1cm long, rooting rate is only 48.1%, and survival rate is only 43.2% after being transferred to the outer hardening of shed.
Embodiment 3：The cottage method of variety classes and concentration HORMONE TREATMENT
According to table 1 in addition to species and concentration selection hormone, other steps and environment keep with reference to embodiment 1 and each treatment
Unanimously, result of the test is shown in Table 1.
The cutting propagation seedling of the hormon of table 1 treatment
| Hormone | Callus grows the time | Rootage duration | Average root is long | Rooting rate | Transplanting survival rate |
| Clear water | 60 days | 80 days | 3.7cm | 11.3% | 9.91% |
| 50mg/L methyl α-naphthyl acetates | 45 days | 55 days | 4.8cm | 57.3% | 45.9% |
| 100mg/L methyl α-naphthyl acetates | 45 days | 50 days | 5.3cm | 63.2% | 55.4% |
| 200mg/L methyl α-naphthyl acetates | 40 days | 50 days | 6.7cm | 71.5% | 66.3% |
| 300mg/L methyl α-naphthyl acetates | 40 days | 50 days | 8.1cm | 86.3% | 80.7% |
| 100mg/L indolebutyric acids | 50 days | 60 days | 4.9cm | 56.1% | 49.7% |
| 200mg/L indolebutyric acids | 50 days | 55 days | 5.7cm | 61.4% | 46.3% |
| 300mg/L indolebutyric acids | 45 days | 55 days | 6.3cm | 66.3% | 53.6% |
As seen from the results in Table 1, suitable growth hormone and its concentration is only selected, can obtains Ilex kudincha C. J. Tseng seedling
Significantly rooting rate high and transplanting survival rate are obtained, and shortens time and rootage duration that callus grows, so as to protect
Growing-seedling period shortening is demonstrate,proved.And the increase of rooting rate ensure that the raising of survival rate, while also improving the life after kind of transplantation of seedlings
Power long.
Embodiment 4：The cottage method of different pH value naphthalene acid solution treatment
According to table 2 in addition to naphthalene acid solution selection different pH values, other steps and environment are with reference to embodiment 1 and everywhere
Reason is consistent, and result of the test is shown in Table 2.
The cutting propagation seedling of the different pH values naphthalene acid solution of table 2 treatment
| Acid-base value | Callus grows the time | Rootage duration | Average root is long | Rooting rate | Transplant survival rate |
| pH 5.0 | 50 days | 70 days | 2.1cm | 15.7% | 11.2% |
| pH 5.5 | 50 days | 65 days | 3.3cm | 27.5% | 19.3% |
| pH 6.0 | 45 days | 55 days | 4.7cm | 67.5% | 53.7% |
| pH 6.5 | 40 days | 50 days | 8.3cm | 85.1% | 79.2% |
| pH 7.0 | 40 days | 55 days | 7.9cm | 75.8% | 64.4% |
| pH 7.5 | 45 days | 60 days | 5.6cm | 35.7% | 23.5% |
| pH 8.0 | 55 days | 75 days | 1.8cm | 18.7% | 9.6% |
As seen from the results in Table 2, the naphthalene acid solution under different pH condition has aobvious to the cutting experiment of Ilex kudincha C. J. Tseng
Influence is write, the pH value that the present invention is limited makes Ilex kudincha C. J. Tseng seedling obtain significant rooting rate high and transplanting survival rate, and
Time and rootage duration that callus grows are shortened, so as to ensure that growing-seedling period shortens, and the increase of rooting rate is protected
The raising of survival rate is demonstrate,proved, while also improving the growing power after kind of transplantation of seedlings.
Embodiment 5：Variety classes and the cottage method of proportioning Nutrition Soil treatment
According to table 3 in addition to species and proportioning selection Nutrition Soil, other steps and environment are protected with reference to embodiment 1 and each treatment
Hold consistent, result of the test is shown in Table 3.
The variety classes of table 3 and the cutting propagation seedling of proportioning Nutrition Soil treatment
It is whole according to the Ilex kudincha C. J. Tseng seedling that cuttage breeding method of the present invention is obtained from the result of the test of table 3
Individual process shortens time and the rootage duration that callus grows, so as to ensure that growing-seedling period shortens, while also improving
Survival rate and growing power after kind transplantation of seedlings.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (8)
1. a kind of cuttage breeding method of Ilex kudincha C. J. Tseng seedling, it is characterised in that including：
Step 1, preparation cuttings；
Step 2, the cuttings that will be made first clean 5min in clear water, and then 30 cuttings bindings are agglomerating, and being immersed in pH value is
Processed more than 8 hours in the naphthalene acid solution of 6.0-7.0, cuttings root is rinsed with clear water after having processed；
Step 3, through methyl α-naphthyl acetate process after cuttings on seedbed cuttage and seedling culture；
Step 4, whne axillary bud germinating in cuttings and root it is long to more than 5cm when transplant, the Nutrition Soil of transplanting is 30% yellow mud, 30% river
Husky, 30% coconut palm chaff and 10% cow dung simultaneously mix the Nutrition Soil for stacking curing more than 6 months after adding 3% composite fertilizer of gross weight,
When cuttings seedling up to 20-25cm long, Ilex kudincha C. J. Tseng seedling is obtained.
2. cuttage breeding method according to claim 1, it is characterised in that step 1 is：
Chosen from the Ilex kudincha C. J. Tseng maternal plant for growing fine and give birth to the branch of semi-lignified as cutting then and be made cuttings, institute
The axillary bud that cuttings retains more than 2 is stated, and retains 3-4 piece blades.
3. cuttage breeding method according to claim 2, it is characterised in that the cuttings retains the axillary bud of more than 2, and guarantor
3-4 pieces are stayed to subtract the blade of 1/3 leaf area.
4. the cuttage breeding method according to claim 1-3 any one, it is characterised in that the cutting length 15-20cm,
Diameter 0.6-1.0cm, at 2.0cm above axillary bud, lower cut is at 5-10cm below axillary bud, and cuttings otch is whole for cuttings upper cut
Neat crack-free.
5. cuttage breeding method according to claim 1, it is characterised in that the pH value is 6.5.
6. cuttage breeding method according to claim 1, it is characterised in that the naphthalene acid solution concentration is 300mg/L.
7. cuttage breeding method according to claim 1, it is characterised in that step 3 is：
Cuttings is inserted on seedbed, seedbed is sprayed using antiseptic before cuttage, cuttings depth is the 1/5 to 1/4 of cuttings overall length, is inserted
Ear density is not overlapped as standard with blade face, after cuttage is complete immediately by seedbed pour it is permeable once；
The plastic, transparent film shed of 50cm high is set up after cuttage on seedbed and double-deck shade net caping is set at top, weekly
Nursery bed disinfection sterilization is sprayed using antiseptic, holding seedbed humidity is periodically watered more than 80%, and controls arch temperature of shed to exist
Between 28-30 DEG C, cuttings nursery sprays foliar fertilizer after 50 days.
8. the cuttage breeding method according to claim 1 or 7, it is characterised in that the seedbed is with river sand as matrix.
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| CN107047203A (en) * | 2017-05-10 | 2017-08-18 | 蚌埠王恒亮盆景艺术有限公司 | A kind of trident maple cuttage seeding cultivating method |
| CN107567917B (en) * | 2017-09-08 | 2020-07-03 | 徐华龙 | Soft branch cuttage breeding method for ilex latifolia |
| CN108200838A (en) * | 2017-12-18 | 2018-06-26 | 杨军贤 | A kind of cultivation implantation methods of hardship Sweet tea tree |
| CN108834708A (en) * | 2018-06-12 | 2018-11-20 | 浦江县元寿农业科技有限公司 | The propagation method of Chinese ilex |
| CN109699436A (en) * | 2019-01-25 | 2019-05-03 | 南京林业大学 | It is a kind of for cultivating the cultivation matrix of ilex verticillata container seedling |
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| CN103416177A (en) * | 2012-05-24 | 2013-12-04 | 韦广荣 | Kuding tea cutting propagation method |
| CN104335791A (en) * | 2013-07-28 | 2015-02-11 | 赵百华 | Potted planting method for broadleaf holly tea with medicinal and ornamental functions |
| CN104396533B (en) * | 2014-12-03 | 2017-07-11 | 大新县生产力促进中心 | The implantation methods of organic Ilex Latifolia Thunb |
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