CN106198448A - A kind of automatic high speed lossless sorting live body male and female Pupa bombycis or the technique of live body male and female Bombyx bombycis - Google Patents
A kind of automatic high speed lossless sorting live body male and female Pupa bombycis or the technique of live body male and female Bombyx bombycis Download PDFInfo
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- 241000382353 Pupa Species 0.000 title claims abstract description 98
- 241000255791 Bombyx Species 0.000 title claims abstract description 89
- 238000000034 method Methods 0.000 title claims abstract description 53
- 238000001228 spectrum Methods 0.000 claims abstract description 75
- 238000001514 detection method Methods 0.000 claims abstract description 16
- 230000005540 biological transmission Effects 0.000 claims abstract description 12
- 230000003595 spectral effect Effects 0.000 claims abstract description 12
- 230000000007 visual effect Effects 0.000 claims abstract description 9
- 230000001678 irradiating effect Effects 0.000 claims abstract description 7
- 238000007781 pre-processing Methods 0.000 claims abstract description 4
- 241000255789 Bombyx mori Species 0.000 claims description 61
- 239000013307 optical fiber Substances 0.000 claims description 17
- 238000002265 electronic spectrum Methods 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 8
- 230000008859 change Effects 0.000 claims description 6
- 230000009466 transformation Effects 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 238000000862 absorption spectrum Methods 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 claims description 4
- JBRZTFJDHDCESZ-UHFFFAOYSA-N AsGa Chemical compound [As]#[Ga] JBRZTFJDHDCESZ-UHFFFAOYSA-N 0.000 claims description 3
- 238000013528 artificial neural network Methods 0.000 claims description 3
- 230000002068 genetic effect Effects 0.000 claims description 3
- 229910052738 indium Inorganic materials 0.000 claims description 3
- APFVFJFRJDLVQX-UHFFFAOYSA-N indium atom Chemical compound [In] APFVFJFRJDLVQX-UHFFFAOYSA-N 0.000 claims description 3
- 238000010239 partial least squares discriminant analysis Methods 0.000 claims description 3
- 238000003909 pattern recognition Methods 0.000 claims description 3
- 230000009467 reduction Effects 0.000 claims description 3
- 239000002210 silicon-based material Substances 0.000 claims description 3
- 238000012706 support-vector machine Methods 0.000 claims description 3
- 238000000149 argon plasma sintering Methods 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 238000012937 correction Methods 0.000 claims description 2
- 238000013461 design Methods 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 description 13
- 238000005516 engineering process Methods 0.000 description 7
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- 238000004611 spectroscopical analysis Methods 0.000 description 5
- 230000005856 abnormality Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 240000000249 Morus alba Species 0.000 description 3
- 206010008190 Cerebrovascular accident Diseases 0.000 description 2
- 235000008708 Morus alba Nutrition 0.000 description 2
- 208000006011 Stroke Diseases 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- RPQDHPTXJYYUPQ-UHFFFAOYSA-N indium arsenide Chemical compound [In]#[As] RPQDHPTXJYYUPQ-UHFFFAOYSA-N 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000010977 jade Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- 238000009366 sericulture Methods 0.000 description 2
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- 210000004392 genitalia Anatomy 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
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- 238000000985 reflectance spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
- B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
- B07C5/02—Measures preceding sorting, e.g. arranging articles in a stream orientating
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
- B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
- B07C5/34—Sorting according to other particular properties
- B07C5/342—Sorting according to other particular properties according to optical properties, e.g. colour
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- G—PHYSICS
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- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N2021/3595—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using FTIR
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Abstract
The present invention relates to a kind of High-Speed Automatic lossless sorting live body male and female Pupa bombycis and live body male and female Bombyx bombycis method.Bombyx bombycis or Pupa bombycis are sent into one by one the spectral detection visual field in the Sex judging system of online automatic sorting device, the light source light sent, produces scattered light or transmission light after irradiating Pupa bombycis or Bombyx bombycis, utilize spectrogrph high speed acquisition live body Bombyx bombycis or the spectrum of Pupa bombycis；After using preprocessing procedures that spectrum is carried out pretreatment, Land use models identification model judges to be sorted the male and female of Pupa bombycis or Bombyx bombycis, and recognition result is sent into sorting system in online automatic sorting device.Sorting system in on-line automatic sorting equipment is responsible for the Sex judging result sent according to online Sex judging system, use mechanical braking or pneumatic mode, respectively male and female Bombyx bombycis or Pupa bombycis are put into different hoppers, it is achieved to male and female Pupa bombycis or the sorting of Bombyx bombycis.The method can realize automatic Undamaged determination and the sorting at a high speed of live body male and female Pupa bombycis or live body male and female Bombyx bombycis.
Description
Technical field
A kind of automatic high speed lossless sorting live body male and female Pupa bombycis or the technique skill of live body male and female Bombyx bombycis are the present invention relates to
Art.This Technology is based on molecule and electronic spectrum information and chemometrics method exploitation, including technological principle, technique
Equipment, spectra collection method and data processing method, it is achieved that live body male and female Pupa bombycis or live body male and female Bombyx bombycis the most lossless
Differentiate and sorting at a high speed.Therefore, the present invention relates to the Pupa bombycis production of hybrid seeds and Bombyx bombycis filature field.
Background technology
China is the source state of world's sericulture industry and propagates state, and silkworm and mulberry and Silk Industry were once that the new and high technology of China is produced
Industry, people's livelihood industry, pillar industry and exploit industry." Silk Road " once erected commerce and trade contact between east and west and the bridge of cultural exchanges
Beam.Since 20 century 70s, the silk manufacturing country of China's maximum the most in the world and exported country, occupy in international market
Absolute predominance, within 2013, mulberry cocoon, silk yield account for the 76.74% and 81.47% of Gross World Product respectively.Silkworm egg is silkworm
Mulberry produces the most basic means of production, the stable and development of silkworm egg industry be realize China's silkworm industry sustainable development important foundation it
One.Currently, silkworm egg production in the whole nation is still that and has continued tradition decades, backward production facility and technological process, the especially production of hybrid seeds
Link is dependent on the operation of manual operations, labor-intensive.The production of Bombyxmori Linnaeus first cross kind is by the most consanguine parent
Carry out copulation, produce heterosis, hybrid vigor.And the discriminating operation of male and female Pupa bombycis is entirely the genitals observing by the naked eye Pupa bombycis afterbody
Official differentiates identification, needs large quantities of manually carrying out, and the vision and proficiency level to male and female personnel respectively has necessarily
Requirement.Along with the aging degree of the in short supply of labour force He operator increases, different silkworm strain in production of hybrid seeds production process
The discriminating operation of male and female pupa has met with the restriction of bottle-neck, and speed of operation is slow, error rate is high, has not only had a strong impact on silkworm egg production
Progress and quality, it is often more important that the silkworm egg of production due to hybridize thorough rate reduce, numerous silkworm raisers raise silkworm egg purity not
Height, yield reduces, and economic benefit is a greater impact.It is in recent years, national that because hybridizing, thorough rate is the highest causes sericulture yield to reduce
Quality events occur repeatedly.The mechanization requirement that production of hybrid seeds phase Sex judging is operated by whole nation silkworm egg industry, the most urgently.To system
Plant phase Sex judging operation and realize mechanization, be not only the technical barrier of China's silkworm egg industry, be also the skill of puzzlement world silkworm egg industry
An art difficult problem.
The yarn quality of male and female Bombyx bombycis has very big difference, the silkworm silk of male Bombyx bombycis to be high-quality silkworm silk, therefore, is divided by male and female Bombyx bombycis
The technology of picking is an important subject in filature industrial circle, and the breakthrough of this technology has great economic worth.At present
Also there is no any relevant Sorting Technique.
Summary of the invention
Difficult for the technology of male and female Bombyx bombycis high-speed lossless sorting in male and female Pupa bombycis in solution Bombyxmori Linnaeus production of hybrid seeds field and filature field
Topic, the invention discloses a kind of High-Speed Automatic lossless sorting live body male and female Pupa bombycis and the Technology of live body male and female Bombyx bombycis.To treat
Sorting living silkworm chrysalises or Bombyx bombycis load feed system, and auto arrangement becomes a team, send into the online acquisition spectrum visual field, high speed acquisition
Its spectrum, relies on the spectral information that its chemical composition produces, use chemometrics method differentiate online male and female Pupa bombycis and
Male and female Bombyx bombycis, and according to identification result and Automated Sorting System, it is achieved the lossless sorting of automatic high speed of Pupa bombycis or Bombyx bombycis.
A kind of High-Speed Automatic lossless sorting live body male and female Pupa bombycis of present invention announcement or the technique skill of live body male and female Bombyx bombycis
Art is characterised by, develops, including technological principle, technological equipment, light based on molecular spectrum information and chemometrics method
Spectrum acquisition method and data processing method.
1. a High-Speed Automatic lossless sorting live body male and female Pupa bombycis and live body male and female Bombyx bombycis technique, it is characterised in that application
Following device, this device includes: feeding funnel (11), feed bin (1), tripper (2), conveyer belt (3), light source (4), optical fiber (5), light
Spectrometer (6), sorting equipment, male silkworm/hero cocoon magazine (9), female silkworm/female cocoon magazine (10), male silkworm pupa/male silkworm cocoon sorting bucket (13),
Female Pupa bombycis/female Bombyx bombycis sorting bucket (14) and roller take off bin (15)；It is followed successively by hopper (11), feed bin (1), sub-material from top to bottom
Device (2), conveyer belt (3)；Conveyer belt is arranged over light source (4), optical fiber (5), and optical fiber (5) connects spectrogrph (6), spectrogrph (6)
Connect data handling system；Spectrogrph (6) includes monochromator and photodetector；Conveyer belt (3) end is provided with sorting equipment, male silkworm
Pupa/male silkworm cocoon sorting bucket (13), female Pupa bombycis/female Bombyx bombycis sorting bucket (14)；Male silkworm pupa/male silkworm cocoon sorting bucket (13), female Pupa bombycis/female
Bombyx bombycis sorting bucket (14) is positioned at roller and takes off the top of bin, and is connected respectively to male silkworm/hero cocoon magazine (9), female silkworm/female cocoon material
Box (10)；
Specifically include following steps:
1) live body Bombyx bombycis or Pupa bombycis being put into feeding funnel (11), using shakes sieves principle in tripper (2) by silkworm to be sorted
Pupa or Bombyx bombycis line up end to end formation, enter conveyer belt；
2) the spectrum inspection during Bombyx bombycis or Pupa bombycis are sent into the Sex judging system of online automatic sorting device by conveyer belt one by one
Survey visual field, the light source light sent, produce scattered light or transmission light after irradiating Pupa bombycis or Bombyx bombycis, utilize spectrometer collection live body
Bombyx bombycis or the spectrum of Pupa bombycis；
3) spectrum gathered is sent into data handling system, after using preprocessing procedures that spectrum is carried out pretreatment,
Land use models identification model judges to be sorted the male and female of Pupa bombycis or Bombyx bombycis, and recognition result is sent into online automatic sorting device
Middle sorting equipment；
4) the Sex judging result that sorting equipment sends according to online Sex judging system, uses mechanical braking or pneumatic
Mode, puts into different hoppers by male and female Bombyx bombycis or Pupa bombycis respectively, it is achieved to male and female Pupa bombycis or the sorting of Bombyx bombycis.
2. further, spectral detection visual field is molecular spectrum or electronic spectrum field of detection.
3. further, tripper (3) have outside from center, design along certain inclination spiral structure, make Pupa bombycis or
Bombyx bombycis moves forward along passage, it is achieved Pupa bombycis or Bombyx bombycis are queued up one by one.
4. further, light source the light sent, produce scattered light or transmission light after irradiating Pupa bombycis or Bombyx bombycis, will by optical fiber
Scattered light or transmission light enter monochromator after collecting, and after monochromator splitting, send into photodetector detection and obtain the molecule light of sample
Spectrum or electronic spectrum.
5. further, monochromator be grating beam splitting, Fourier transformation light splitting, acousto-optic turnable filter light splitting, optical filter or
Light emitting diode；Detector is silicon materials or the photoelectric detector of indium gallium arsenic material.
6. further, molecular spectrum wave-length coverage is to cover 700-2500nm or 400-4000cm-1Full spectrum, or special
Levy wavelength or characteristic wave bands, or the combination between them.Change resolution scope 0.5-126cm of molecular spectrum-1。
Electronic spectrum wave-length coverage can be the full spectrum covering 200-700nm, it is also possible to be characteristic wavelength or characteristic wave bands,
Or the combination between them.Change resolution scope 0.5-126cm of electronic spectrum-1.Spectral form be energy curve or
It is absorption spectrum or transmitance, reflectance or interferogram.
7. further, the preprocess method that described step (3) is used includes: differential, smooth, light scattering correction, standard
Normal variate conversion, average centralization, principle component analysis data dimensionality reduction, wavelet transformation analysis, genetic algorithm, moisture deduction arithmetic
Any one of, or wherein between combination in any.
8. further, extract live body male and female Pupa bombycis or live body male and female Bombyx bombycis respective characteristic spectrum information, use pattern
Live body male and female Pupa bombycis identification model or live body male and female Bombyx bombycis identification model are set up in recognition methods.Will living silkworm chrysalises be sorted or
Live body Bombyx bombycis molecular spectrum and electronic spectrum input this model, it determines its sex.Used mode identification method include PCA, away from
From coefficient of similarity method, bunch class independence soft mode formula, partial least squares discriminant analysis method, K closest to method, FISHER linear discriminant,
Any one in artificial neural network and support vector machine, or wherein between combination in any.
Accompanying drawing explanation
Fig. 1 live body Bombyx bombycis/Pupa bombycis on-line automatic sorting equipment front elevation
Fig. 2 Bombyx bombycis/Pupa bombycis on-line automatic sorting equipment male and female sorting bucket and magazine partial right side face figure
Fig. 3 tripper helical structure top view
The original near-infrared of Fig. 4 living silkworm chrysalises spectrogram (A) female (B) that diffuses is male
The projection of Fig. 5 living silkworm chrysalises three-dimensional feature and mahalanobis distance
(A) female three-dimensional feature projection (B) male three-dimensional feature projection (C) female mahalanobis distance (D) male mahalanobis distance
Fig. 6 living silkworm chrysalises SIMCA classification chart (A) female (B) is male
The original near-infrared of Fig. 7 live body Bombyx bombycis spectrogram (A) female (B) that diffuses is male
Fig. 8 live body Bombyx bombycis main constituent three-dimensional feature projection (A) female (B) is male
Fig. 9 live body Bombyx bombycis SIMCA classification results (A) female (B) is male
Figure 10 process chart
Detailed description of the invention:
1), when Pupa bombycis to be sorted or Bombyx bombycis fall into tripper (2) after feed bin (1), end to end formation is lined up；
2) by (3) transporter, Bombyx bombycis or Pupa bombycis are sent into one by one in the Sex judging system of online automatic sorting device
Spectral detection visual field, utilize near-infrared or ultraviolet-visual spectrometer, gather live body Bombyx bombycis or the spectrum of Pupa bombycis.Specific as follows:
Light that light source sends produces scattered light or transmission light after irradiating Pupa bombycis or Bombyx bombycis, by optical fiber (5) by scattered light or
Transmission light enters the monochromator in (6) spectrogrph after collecting, and after monochromator splitting, sends into the detection of the photodetector in (6) and obtains
The spectrum of sample.Or the light that light source sends is introduced into the monochromator in (6), after monochromator splitting, light irradiates Pupa bombycis or silkworm
Produce scattered light or transmission light after cocoon, optical fiber (5) after scattered light or transmission light being collected, enter the photodetector detection in (6)
Obtain the spectrum of sample.
Monochromator can be grating beam splitting, Fourier transformation light splitting, acousto-optic turnable filter (AOTF) light splitting, optical filter,
Light emitting diode.Detector can be silicon materials or the photoelectric detector of indium gallium arsenic material.
Molecular spectrum wave-length coverage can be to cover 700-2500nm or 400-4000cm-1Full spectrum, it is also possible to be feature
Wavelength or characteristic wave bands, or characteristic wavelength and the combination of characteristic wave bands.Change resolution scope 0.5-of molecular spectrum
126cm-1Electronic spectrum wave-length coverage can be the full spectrum covering 200-700nm, it is also possible to can also be characteristic wavelength or feature
Wave band, or characteristic wavelength and the combination of characteristic wave bands.Change resolution scope 0.5-126cm of electronic spectrum-1.Spectral form
Can be energy curve or absorption spectrum or transmitance or interferogram.The change resolution of electronic spectrum
Scope 0.5-126cm-1。
Spectral form can be energy curve or absorption spectrum or transmitance, reflectance or interference
Figure.
Gathering spectroscopy mode can be diffuse-reflectance, it is also possible to be diffusing transmission；In Spectroscopy procedures, sample is in motion shape
State；Each sample spectra acquisition time≤250ms, identification >=4 per second.
3) will gather spectrum send into data handling system, as each sample collection be 2 with polishing wax, by each sample
This spectrum is averaged, and uses use preprocessing procedures when setting up identification model that averaged spectrum is carried out spectrum and locates in advance
Reason.Pretreated spectrum substitution male and female pattern recognition model is carried out male and female identification, and recognition result is sent into on-line automatic
The mechanism for sorting in sorting system in sorting equipment.
Preprocess method is specific as follows: use differential, smooth, MSC, average centralization, PCA data dimensionality reduction, wavelet transformation
Analysis, genetic algorithm, moisture deduction any of which, or wherein between combination in any carry out Pretreated spectra.
Identify that model is set up used mode identification method and included: PCA, Distance conformability degree Y-factor method Y, bunch class independence soft mode
Formula (SIMCA), partial least squares discriminant analysis method (PLS-DA), K are closest to method, FISHER linear discriminant, artificial neural network
With any one in support vector machine, or combination in any.Can manually set up can also be by data handling system
Automatic modeling function is set up automatically.Modeling specifically comprises the following steps that
1. prepare >=50 known sexes female Pupa bombycis and male Pupa bombycis sample, or female Bombyx bombycis and male Bombyx bombycis sample
This, be divided into calibration set and checking collection by sample, and calibration set is used for setting up male and female pattern recognition model, and checking collection is used for verifying model
Recognition correct rate；
2. the spectrum of the on-line automatic collecting sample of on-line automatic sorting equipment is utilized；
3. spectrum is carried out pretreatment；
4. modeling best features spectral coverage is selected；
5. identify exceptional value and reject；
6. Land use models recognition methods sets modeling parameters, sets up and identifies model, the prediction accuracy of computation model.If it is female
Male prediction accuracy >=95%, then model can be used.If prediction accuracy < 95%, then reset modeling parameters, until male and female are pre-
Survey accuracy >=95%.
If prediction accuracy can not reach 95%, then from 3. restarting, until model prediction rate >=95%.
If prediction accuracy can not reach 95%, then from 1. restarting, until model prediction rate >=95%.
4) mechanism for sorting in the sorting system in on-line automatic sorting equipment is responsible for sending out according to online Sex judging equipment
The Sex judging result gone out, uses mechanical braking or pneumatic mode, male and female Bombyx bombycis or Pupa bombycis is put into different (9) respectively
(10) magazine, it is achieved to male and female Pupa bombycis or the sorting of Bombyx bombycis.
(1) 1 40000 male and female Pupa bombycis sortings of embodiment
The present embodiment has carried out the sorting of 40,000 Pupa bombycis, and sorting male and female accuracy respectively reaches 97%, 99%.
The automatic sorting device Facad structure that the present embodiment uses is as it is shown in figure 1, use near-infrared light source and gas purging
Mode sorts.Composition includes feeding funnel (11) feed bin (1) tripper (2) conveyer belt (3) near-infrared light source (4) optical fiber (5)
Spectrogrph (6) reference (12) data handling system (7) inflatable bar (8) male silkworm/hero cocoon magazine (9) female silkworm/female cocoon magazine (10) is male
Pupa bombycis/male silkworm cocoon sorting bucket (13) female Pupa bombycis/female Bombyx bombycis sorting bucket (14) roller takes off bin (15).(9)(10)(13)(14)
(15) partial right side face structure is as shown in Figure 2.(2) tripper helical structure top view is as it is shown on figure 3, helix angle is 3-5 degree.
The step of Pupa bombycis sorting is as follows:
1) a batch living silkworm chrysalises is put in the feed system feeding funnel (11) of online automatic sorting device, silkworm to be sorted
When pupa or Bombyx bombycis fall into feed bin (1) bottom-right tripper (2) after the feed bin (1) of (11) lower section, line up end to end
Formation；
2) by the conveyer belt (3) of tripper (2) lower section, Bombyx bombycis or Pupa bombycis are sent into online automatic sorting device one by one
Spectral detection visual field in Sex judging system, utilizes near infrared spectrometer and optical fiber, gathers live body Bombyx bombycis or the spectrum of Pupa bombycis.
Specific as follows:
The light sent by near-infrared light source (4), produces scattering after the Pupa bombycis of irradiation conveyer belt (4) upper and lower or Bombyx bombycis
Light, the optical fiber (5) being arranged on above illuminated Pupa bombycis enter the spectrogrph (6) being connected with optical fiber (5) after scattered light being collected
In monochromator, after monochromator splitting, send into the detection of the photodetector in spectrogrph (6) and obtain the spectrum of sample.Monochromator is
Grating beam splitting, detector is the photoelectric detector of indium arsenic material.A reference is measured every 1 hour.Reference (12) is placed
In conveyer belt (4) lower left, during measurement, reference by conveyer belt (4) lower left through mechanical braking move to conveyer belt (4) just under
Side, after reference measurement is complete, recalls in situ.
Spectrum ripple acquisition range is 900-1700nm, and resolution is 2nm, and gathering spectroscopy mode is diffuse-reflectance pattern.
In Spectroscopy procedures, sample is kept in motion；Set spectra collection speed as 67 spectrum/seconds, Mei Gecan
Pupa gathers 2-5 and opens spectrum, and (depending on showing Pupa bombycis size), the most each Pupa bombycis acquisition time is 30～75ms, measurement 13～33 per second
Individual Pupa bombycis.
3) spectrum gathered is sent into data handling system (7), after deduction reference spectrum, use SNV and second dervative to enter
Row Pretreated spectra, then uses the SIMCA model of foundation to carry out male and female differentiation.Concrete modeling process is as follows:
(1) sample collection and spectra collection
Collect autumn wind, white jade, wild three kinds of ternary totally 600 live body male and female Pupa bombycises, each 300 of male and female.Use this reality
Execute example step 2) in method gather spectrum, spectrum is as shown in Figure 4.
(2) Pretreated spectra
The present embodiment uses SNV and second dervative to carry out Pretreated spectra.
(3) abnormality value removing.
Use the projection of main constituent three-dimensional feature and mahalanobis distance method rejecting abnormalities sample.At main constituent three-dimensional feature projection sky
It is modeling sample within ellipse between, is outliers outside ellipse, use mahalanobis distance to arrange when calculating outliers and peel off
Threshold value is 1.7, and mahalanobis distance is considered exceptional value more than 1.7.Fig. 5 is the projection of living silkworm chrysalises three-dimensional feature and mahalanobis distance.
(4) SIMCA model is set up.
Modeling number of principal components is selected according to validation-cross PRESS and principal component contributor rate.After selecting PRESS flex point, main
The composition accumulation contribution rate number of principal components to more than 99%.Optimal number of principal components that this example is established is female is 18, male
It is 20.SIMCA is for live body male and female Pupa bombycis classifying quality as shown in Figure 6.In Fig. 6, Q-value is the sample distance away from model；T2For sample
This influence degree to model.On the premise of not considering that male and female classification is to pattern representation degree, comparative sample and apoplexy due to endogenous wind other
The difference of sample；Intermediate vertical line and horizontal line provide the confidence interval being given under 0.025 significant level, two straight lines and coordinate
The region that axle surrounds is the effective coverage of respective attributes.Model prediction accuracy is 97.5%.
4) recognition result is sent into inflatable bar (8), use pneumatic mode to be blown into by male and female Pupa bombycis respectively and be arranged on (3) terminal
Male silkworm pupa/male silkworm cocoon sorting bucket (13) female Pupa bombycis/female Bombyx bombycis sorting bucket (14) at place, later falls into and is placed on roller and takes off bin
(15) male silkworm above/hero cocoon magazine (9) and female silkworm/female cocoon magazine (10), it is achieved the sorting to this batch of male and female Pupa bombycis.
Embodiment two male and female Bombyx bombycis sorts
The present embodiment has sorted 1000 live body Bombyx bombycis, and sorting accuracy male and female accuracy respectively reaches 96%, 97%.
The automatic sorting device that the present embodiment uses is with embodiment 1.
Sorting specifically comprises the following steps that
1) a batch live body Bombyx bombycis is put in the feed system feeding funnel (11) of online automatic sorting device, silkworm to be sorted
When pupa or Bombyx bombycis fall into bottom-right tripper (2) after the feed bin (1) of (11) lower section, line up end to end formation；
2) spectrum sent into one by one by Bombyx bombycis by conveyer belt (3) in the Sex judging system of online automatic sorting device is examined
Survey visual field, utilize near infrared spectrometer and optical fiber, gather the spectrum of live body Bombyx bombycis.Specific as follows:
The light sent by near-infrared light source, produces scattered light, by being arranged on after irradiating on conveyer belt the Bombyx bombycis below light source
Optical fiber (5) above illuminated Bombyx bombycis enters the monochromator in the spectrogrph (6) being connected with optical fiber (5) after scattered light being collected,
After monochromator splitting, send into the detection of the photodetector in spectrogrph (6) and obtain the spectrum of sample.Monochromator is grating beam splitting, inspection
Survey the photoelectric detector that device is indium arsenic material.
Spectrum ripple acquisition range is 900-1700nm, and resolution is 2nm, and gathering spectroscopy mode is diffuse-reflectance pattern.
Spectrum ripple acquisition range is 1000-1800nm, and resolution is 2nm, and spectrum is near-infrared diffuse-reflectance spectrum.Spectrum is surveyed
During amount, sample is kept in motion；Setting spectra collection speed as 70/second, each sample collecting 4-8 opens spectrum (tool
Depending on stereoscopic sample size), the most each Pupa bombycis acquisition time is 58～125ms, measurement 8 per second～17 Pupa bombycises.
3) spectrum gathered is sent into data handling system (7), use SNV and second order to lead and spectrum is carried out pretreatment.So
The rear SIMCA disaggregated model utilizing foundation is identified classification.Concrete modeling process is as follows:
(1) sample collection and spectra collection
Collect autumn wind, white jade, wild three kinds of ternary totally 200 live body male and female Bombyx bombycis, each 100 of male and female.Use this reality
Execute example step 2) in method gather spectrum, spectrum is as shown in Figure 7.
(2) Pretreated spectra
SNV and second dervative is used to carry out Pretreated spectra.
(3) abnormality value removing.
The projection of main constituent three-dimensional feature is used to carry out abnormality value removing.In main constituent three-dimensional feature projector space oval with
Interior is modeling sample, is exceptional sample outside ellipse.Three-dimensional feature projector space is as shown in Figure 8.
(4) SIMCA model is set up.
Modeling number of principal components is selected according to validation-cross PRESS and principal component contributor rate.After selecting PRESS flex point, main
The composition accumulation contribution rate number of principal components to more than 99%.Optimal number of principal components that this example is established is female is 10, male
It is 9.SIMCA is for live body male and female Pupa bombycis classifying quality as shown in Figure 9.Before not considering that male and female classification is to pattern representation degree
Putting, comparative sample is different from other samples of apoplexy due to endogenous wind；Intermediate vertical line and horizontal line are given and are given under 0.025 significant level
Confidence interval, the region that two straight lines and coordinate axes surround is the effective coverage of respective attributes.Model Identification accuracy is
98.0%.
4) recognition result is sent into inflatable bar (8), use pneumatic mode to be blown into by male and female Bombyx bombycis respectively and be arranged on (3) terminal
Male silkworm pupa/male silkworm cocoon sorting bucket (13) female Pupa bombycis/female Bombyx bombycis sorting bucket (14) at place, later falls into and is placed on roller and takes off bin
(15) male silkworm above/hero cocoon magazine (9) and female silkworm/female cocoon magazine (10), it is achieved the sorting to this batch of male and female Bombyx bombycis.
Claims (8)
1. a High-Speed Automatic lossless sorting live body male and female Pupa bombycis and live body male and female Bombyx bombycis technique, it is characterised in that below application
Device, this device includes: feeding funnel (11), feed bin (1), tripper (2), conveyer belt (3), light source (4), optical fiber (5), spectrogrph
(6), sorting equipment, male silkworm/hero cocoon magazine (9), female silkworm/female cocoon magazine (10), male silkworm pupa/male silkworm cocoon sorting bucket (13), female silkworm
Pupa/female Bombyx bombycis sorting bucket (14) and roller take off bin (15)；It is followed successively by feeding funnel (11), feed bin (1), tripper from top to bottom
(2), conveyer belt (3)；Conveyer belt is arranged over light source (4), optical fiber (5), and optical fiber (5) connects spectrogrph (6), and spectrogrph (6) is even
Connect data handling system；Spectrogrph (6) includes monochromator and photodetector；Conveyer belt (3) end is provided with sorting equipment, male silkworm
Pupa/male silkworm cocoon sorting bucket (13), female Pupa bombycis/female Bombyx bombycis sorting bucket (14)；Male silkworm pupa/male silkworm cocoon sorting bucket (13), female Pupa bombycis/female
Bombyx bombycis sorting bucket (14) is positioned at roller and takes off the top of bin, and is connected respectively to male silkworm/hero cocoon magazine (9), female silkworm/female cocoon material
Box (10)；
Specifically include following steps:
1) live body Bombyx bombycis or Pupa bombycis are put into feeding funnel (11), use shake sieve principle in tripper (2) by Pupa bombycis to be sorted or
Person's Bombyx bombycis lines up end to end formation, enters conveyer belt；
2) spectral detection during Bombyx bombycis or Pupa bombycis are sent into the Sex judging system of online automatic sorting device by conveyer belt one by one regards
, light source the light sent, produce scattered light or transmission light after irradiating Pupa bombycis or Bombyx bombycis, utilize spectrometer collection live body Bombyx bombycis
Or the spectrum of Pupa bombycis；
3) spectrum gathered is sent into data handling system, after using preprocessing procedures that spectrum is carried out pretreatment, utilize
Pattern recognition model judges to be sorted the male and female of Pupa bombycis or Bombyx bombycis, and is sent into by recognition result in online automatic sorting device point
Pick device；
4) the Sex judging result that sorting equipment sends according to online Sex judging system, uses mechanical braking or pneumatic side
Formula, puts into different hoppers by male and female Bombyx bombycis or Pupa bombycis respectively, it is achieved to male and female Pupa bombycis or the sorting of Bombyx bombycis.
Technique the most according to claim 1, it is characterised in that spectral detection visual field is molecular spectrum or electronic spectrum detection
Visual field.
Technique the most according to claim 1, it is characterised in that tripper (2) has outside from center, along certain inclination angle
Helical structure designs, and makes Pupa bombycis or Bombyx bombycis move forward along passage, it is achieved Pupa bombycis or Bombyx bombycis are queued up one by one.
Technique the most according to claim 1, it is characterised in that the light sent by light source, produces after irradiating Pupa bombycis or Bombyx bombycis
Scattered light or transmission light, optical fiber enter monochromator after scattered light or transmission light being collected, and after monochromator splitting, sends into light detection
Device detection obtains molecular spectrum or the electronic spectrum of sample.
Technique the most according to claim 1, it is characterised in that monochromator is grating beam splitting, Fourier transformation light splitting, acousto-optic
Tunable optical filter light splitting, optical filter or light emitting diode；Detector is silicon materials or the Photoelectric Detection of indium gallium arsenic material
Device.
Technique the most according to claim 1, it is characterised in that molecular spectrum wave-length coverage be cover 700-2500nm or
400-4000cm-1Full spectrum, or characteristic wavelength or characteristic wave bands, or the combination between them；The resolution of molecular spectrum
Rate excursion 0.5-126cm-1；Electronic spectrum wave-length coverage is the full spectrum covering 200-700nm, or characteristic wavelength or spy
Levy wave band, or the combination between them；Change resolution scope 0.5-126cm of electronic spectrum-1；Spectral form is that energy is bent
Line or absorption spectrum or transmitance, reflectance or interferogram.
Technique the most according to claim 1, it is characterised in that the preprocess method that described step (3) is used includes: micro-
Point, smooth, light scattering correction, standard normal variable conversion, average centralization, principle component analysis data dimensionality reduction, wavelet transformation divide
Any one of analysis, genetic algorithm, moisture deduction arithmetic, or wherein between combination in any.
Technique the most according to claim 1, it is characterised in that extraction live body male and female Pupa bombycis or live body male and female Bombyx bombycis are each
Characteristic spectrum information, use mode identification method set up live body male and female Pupa bombycis identification model or live body male and female Bombyx bombycis identification mould
Type；Living silkworm chrysalises to be sorted or live body Bombyx bombycis molecular spectrum and electronic spectrum are inputted this model, it determines its sex；Used
Mode identification method includes that PCA, Distance conformability degree Y-factor method Y, bunch class independence soft mode formula, partial least squares discriminant analysis method, K are
Close in method, FISHER linear discriminant, artificial neural network and support vector machine any one, or wherein between any
Combination.
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Inventor after: Yuan Hongfu Inventor after: Zhu Zhiqiang Inventor after: Cheng Teng Inventor after: Hu Aiqin Inventor after: Zhu Xinye Inventor after: Song Chunfeng Inventor after: Yang Yong Inventor before: Yuan Hongfu Inventor before: Zhu Zhiqiang Inventor before: Cheng Teng Inventor before: Hu Aiqin Inventor before: Zhu Xinye Inventor before: Song Chunfeng Inventor before: Yang Yong |
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Application publication date: 20161207 |