JP2007071620A - Method and device for determining sex of silkworm pupa - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method and device for non-invasively determining the sex of each of a large number of silkworm pupae in a short period of time without damaging them in determination work, as assortment is performed by determining the sexes of two kinds of pure breeds to obtain silkworms of a crossbred F1 having a high cocoon yield. <P>SOLUTION: A light beam is projected on the living body of a silkworm pupa by using a light projection means to receive transmitted light coming out of the body of the silkworm pupa by a light receiving means. The transmitted light received is guided through optical fiber to a spectral separation means to be spectrally separated. A spectrum thus obtained is analyzed to digitize light data on a specific waveband. In order to separate females from males, the digitized data are compared with a previously set threshold to output either a female signal or a male one for assortment. Silkworm pupae are one by one separately transferred to an inspection position by using a transfer means, and light is projected onto the inspection position while transmitted light is received. An automatic alignment/supply device is combined with the transfer means to supply the silkworm pupae. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  In the sericulture industry, in order to obtain good quality varieties in the sericulture industry, the pods are discriminated between males and females by projecting light rays into the cocoon or taking out the cocoons from the cocoons, and spectroscopically analyzing reflected or transmitted light. It is related with the method and apparatus which discriminate | determine a male and female efficiently noninvasively.

  In the sericulture farmers, in order to increase the yield of cocoons, hybrids (F1) are bred, and in order to obtain this hybrid (F1), males and females are separated for each variety of the original species until they become cocoons. A hybrid (F1) is bred by mating females and males of different original species.

  For this reason, the sex identification of moths is an indispensable task in the production of moth species, and as a method of sexual identification, there is a method of visual discrimination based on larvae or external sexual characteristics, mottle or color of moths, and the weight of pods and seed moths. There is a method of sorting using a sorter (see, for example, Patent Document 1). In addition, there is a method for discriminating between males and females using a specific gravity difference between males and females by placing the salmon in salt water whose specific gravity has been adjusted in advance (see, for example, Patent Document 2).

In addition, as a method for separating male and female silkworm cocoons, there is a method in which silkworm cocoons are bred under specific short-day sunlight conditions that do not exist in the natural state, and both are separated using the difference in male and female emergence times (for example, patent documents). 3).
Japanese Patent Publication No.43-13671 Japanese Patent Publication No.55-16610 Japanese Patent Publication No.54-30949

The seed and sex selecting machine described in the above-mentioned Patent Document 1 is a device that automatically measures the weight of a living body by a mechanical device and separates a male and a female based on the size of the weight because of the fact that the male pod is generally heavier than the male pod. There was a problem that was not sufficient in terms of accuracy.
That is, there are weight distributions due to individual differences in both females and males, and the weights of small females and large males are mixed, and there is a problem that males and females cannot be accurately separated only by weight.
Furthermore, there is a problem that the weighing device itself cannot be accurately separated by the seed / sex selector using the weighing device due to variations in weighing accuracy.

Next, the male / female discrimination method described in Patent Document 2 is a method for discriminating between males and females by utilizing the specific gravity of females and males in a living body.
In other words, it is a method in which a living body is placed in salt water adjusted to have a specific gravity, and the living body that sinks in the salt water is identified as a female, and a floating living body is identified as a male. There was a problem that after taking out and putting in salt water and putting it in salt water, if the air bubbles attached to the skin of the rod body were not removed, the specific gravity was affected and the female would rise.
In addition, since a slight difference in specific gravity is used, if salt water flows, it will affect the rise and sink.Therefore, if salt water is not stabilized in a stationary state, it will not be possible to distinguish the rise and sink. There was such a problem.
That is, even if it can be distinguished on a laboratory scale, there is a problem that it is difficult to put it to practical use in the field where seeds are collected.
In addition, there was a problem that we felt resistance to practical use when we considered the process of rinsing the salt water after putting the living straw in salt water.

Next, the method for separating male and female silkworm cocoons described in Patent Document 3 is a method in which silkworm cocoons are bred under specific short-day photoperiod conditions that do not exist in the natural state, and both are separated by the difference in emergence time between females and males. The pupae that emerged from midnight to early morning are males, and the pupae that emerge from morning to day are separated as females.
That is, it is separated only when it emerges and becomes a cocoon, and it is impossible to separate males and females in the state of the cocoon before they emerge.
In addition, the silkworm cocoon that emerged and turned into a silkworm cocoon moved around and was easily damaged. Therefore, there was a problem that handling for separation was not easy.
That is, in the case of cocoons and cocoons, since they are solid and stationary, they can be handled by any method, but the cocoons that emerged are separated and cannot be handled arbitrarily.
Furthermore, this method has a problem in that it is not possible to discriminate between males and females in the state of a bag or bag that is easy to handle.

  The object of the present invention has been made in view of such facts, and the cocoons are superior, and the cocoons are kept in the cocoon for a short period from the formation of the cocoon in the cocoon to the start of the cocoon, or A method and apparatus for discriminating and discriminating the body of a rod by projecting it from the cage, projecting the light beam non-invasively without damaging or damaging the cage, and spectroscopically analyzing the diffuse reflected light or transmitted light. Is to provide.

  The present invention projects a light beam including visible light to near-infrared light on a living body sample taken out from a sputum, and the projected light is transmitted through the subject or the projected light beam is transmitted. The diffuse reflected light that is diffusely reflected in the subject is received by the light receiving head, the received light is dispersed using the spectroscopic means, the spectrum is analyzed, and the optical data is digitized by multivariate analysis. It is characterized in that it is determined whether it is female or male based on a preset threshold value for separating the male and female, and a determination result signal is output for determination.

The test subject's sputum is taken out of the sputum and used. It is a cocoon before it emerges and becomes a cocoon, but it is alive but has little movement, so it is easy to handle, and this specimen is supplied to the examination position one by one in a fixed posture by the supply means. A light beam having a continuous wavelength including near-infrared light, for example, a light projecting unit using a small mini-halogen lamp is projected onto the subject at the examination position.
This projected light beam is projected with a focus so that it does not spread outside the size of the outer shape of the subject. Since the body has a body width of about 8 mm to 11 mm, it is preferable to use a light projecting means having a light projecting port of the same size or less.

The light receiving means for receiving the transmitted light from the subject or the diffuse reflected light from the inside of the subject guides the light received by the light receiving head to the spectroscopic means using the optical fiber.
The light receiving head is formed so as to have a smaller aperture than the size of the outer shape of the subject, and is configured to collect light beams having sex information through the subject.
The light receiving port of the light receiving head is preferably provided at a position close to the subject.
This light receiving head is preferably provided in a pair with the light projecting port of the light projecting means.

The spectroscopic means splits and photoelectrically converts the light beam received by the light receiving head and guided by the optical fiber, and outputs the spectral spectrum to the arithmetic processing unit.
The arithmetic processing unit analyzes the spectral spectrum by multivariate analysis, and digitizes optical data in a specific wavelength band by statistical analysis.
The numerical value obtained by this analysis is compared with a sex discrimination threshold preset in the setting unit of the arithmetic processing unit, so that the sex is discriminated and either a female or male signal is output.

  The apparatus for discriminating between male and female moths according to the present invention transfers a subject one by one in a predetermined posture using a transfer means, and sets a predetermined position in the transfer process as an inspection position on the transfer means at the inspection position. A light projecting means for projecting a light beam to the subject and a light receiving means for receiving the transmitted light from the subject or the diffuse reflection light emitted from the inside are provided to split the received light and photoelectrically convert the spectrum. Then, it is configured to discriminate between males and females by performing spectral analysis using an arithmetic processing unit, and to output either female or male sorting signals.

The transfer means may be an intermittent transfer method, a continuous transfer (non-stop) method, or a cylindrical alignment transfer body inclined so that the subject naturally slides, as long as the subject is separated and transferred one by one. A device that transports by a chute method may be used, and the subject may be transported on a tray or tray, or may be moved by sliding a cylindrical chute with its own weight under its own weight.
In the middle of the transfer path of the transfer means, a predetermined position is set as an inspection position, and a light projecting means for projecting a light beam onto the body of the subject at the inspection position, a transmitted light or a diffuse transmitted light from the subject is received. The light receiving means is provided so that it can be inspected online.

  The light projecting means for projecting a light beam on the subject is provided in close proximity to the subject at the examination position in association with the transfer means so that the projected light beam is efficiently projected into the subject. . In order to bring the light projection port close, a projecting mechanism may be provided.

  The light receiving means is provided in close proximity to the subject at the examination position in association with the transfer means so as to efficiently receive the light beam coming out of the subject. You may comprise the protrusion mechanism which makes a light-receiving port approach or circumscribe a subject.

  In addition, when the transfer means uses an intermittent transfer method, an intrusion mechanism for simultaneously bringing the light projecting port of the light projecting unit and the light receiving port of the light receiving unit close to or circumscribing the subject while the subject is temporarily stopped is provided. It may be configured so that it is not affected by ambient light.

  When discriminating the subject online, the transport means transports the subject in a predetermined posture and travels through the examination position, and transmits light from the subject in a non-stop manner by matching the timing between the examination position and the subject. Alternatively, it is configured to receive diffusely reflected light. When discriminating a large amount of soot, an automatic alignment feeder such as an electromagnetic vibrator feeder is used in the preceding process of the transfer means, and the cylindrical alignment transfer body (tilt is tilted so that the subject naturally slides on the transfer means ( It is characterized in that a large amount of discrimination processing can be performed at high speed if combined using a shoot).

  The method and apparatus for discriminating males and females of the spider according to the present invention configured as described above project a light beam non-invasively on the living body of the carcass taken out of the carcass, and transmit light from the carcass or diffused from the inside The male and female can be discriminated by receiving the reflected light and performing spectroscopic analysis. Therefore, the male and female can be easily discriminated without giving an impact, damage or load to saltwater.

  A device that projects light beams one by one at the examination position, receives the transmitted light from the subject, and performs spectral analysis can be easily performed anywhere regardless of location or time because the sex can be discriminated by a simple method.

  In addition, the specimens are transported separately one by one, the light is projected during the transportation, the transmitted light or diffuse reflected light from the specimen is received on-line, and the spectroscopic analysis is performed on-line by automating sex determination. You can sort female and male.

  Receives transmitted light or diffusely reflected light from the subject, spectrally analyzes the spectral spectrum with an arithmetic processing unit, digitizes the optical data, and compares the sex with a male / female discrimination threshold to discriminate between males and females. Since such a signal is output, it is possible to accurately determine even if there is no experience or skill of an inspector or the like.

  Since the range below the size of the subject is projected at the examination position, and the range below the size of the subject is received as the field of view, the influence of the disturbance light can be reduced and the discrimination can be made with high accuracy.

  If a transporting means that uses a pan on which the subject is placed in a predetermined posture is used, the orientation of the back, abdomen, head, tail, etc. of the subject to the pan that is stopped in the previous process of the examination position is used. Therefore, the inspection conditions for light projection and light reception are constant, and the discrimination accuracy can be improved.

  In the case of using a cylindrical alignment transfer body inclined to the transfer means, if the cylindrical alignment transfer body is made of a black material that does not transmit light, the influence of disturbance light can be prevented and the accuracy can be improved. Can be distinguished well.

Furthermore, in the case of intermittently transferring the saucer, when the saucer is stopped, the projection port of the light projecting means is brought close to or circumscribed outside the subject, so that the projected light is concentratedly projected onto the subject so as not to leak outside. Efficient light projection.
Further, if the light receiving port of the light receiving means is configured to be close to or circumscribed to the subject, only the light emitted from the subject can be received without being affected by disturbance light.

  Furthermore, if the projector and the light receiving port are configured to circumscribe the subject at the same timing while the pan is stopped, the external disturbance light is shielded and only the light emitted from the subject is received. Since the spectroscopic analysis is performed, the sex can be discriminated with high accuracy.

  When the specimen is continuously moved and transported, and the specimen passes through the examination position, it is configured to receive light non-stop in time, so the processing speed is fast and the efficiency is high, so a large number of specimens are identified and processed. it can.

A light beam is projected onto a subject, which is a living body of a casing supplied to an examination position, using a light projecting unit. The light that has been projected is transmitted through the body of the subject, or diffusely reflected light that is diffused and reflected within the body is received by the light receiving head of the light receiving means.
Transmitted light received by the light receiving head or diffusely reflected light coming from the inside (hereinafter, diffused reflected light is also included in the transmitted light and expressed as transmitted light) is guided to the spectroscopic means through the optical fiber.

The projecting light beam uses a light projecting head configured to project toward the housing using a light projecting lamp that emits light beams including visible light to near infrared light. The light beam is a light beam such as a halogen lamp using a tungsten filament or the like. That is, it refers to light having a wavelength continuously changed by 1 nm. In the present invention, the description will be made using a halogen lamp. However, for example, light having a wavelength continuously changed by 1 nm in a range from 600 nm to 1100 nm may be used. The projection lamp may be any lamp that has a projection range of about the size of the subject. Moreover, you may comprise so that the said light ray may be projected using an optical fiber. Furthermore, the projection range may be narrowed down and projected using a light projection lens.
The projected light beam penetrates the inside of the housing, passes through the inside of the body, or diffusely reflects and comes out of the body as transmitted light having internal information, so that the transmitted light is received by the light receiving head.

As the light receiving head of the light receiving means, it is preferable to use a light receiving head in which an infinite number of optical fiber bundles are bundled and a light receiving surface is mirror-finished to form a light receiving surface. Alternatively, a light receiving head formed so as to receive transmitted light may be used.
The light receiving head is formed with a diameter smaller than the size of the outer shape of the casing, which is the subject, and is provided at a position close to the subject. That is, the disturbance light that does not pass through the subject is formed so as not to enter the light receiving surface. For this purpose, it is preferable to shield the light receiving head from ambient light.

When using an intermittent transfer means for temporarily stopping the subject at the examination position, if the intrusion mechanism is provided so that the light receiving head is circumscribed to the subject at the time of suspension, only the transmitted light from the subject is provided. Can be configured to receive light.
Further, the subject may be softly pressed and moved so as to circumscribe or approach the light receiving surface of the light receiving head.
In addition, there is a difference in size of the body of the subject, and in order to reduce fluctuations in the distance between the light receiving surface of the light receiving head and the subject (also referred to as the light receiving distance), the light receiving head is provided with the surface on which the subject is placed. It is preferable to provide on this receiving surface side with respect to (receiving surface).

The light receiving surface of the light receiving head is preferably provided so as to face the subject with the light projecting head, but the light beam projected into the subject diffuses and reflects within the subject. Therefore, the light receiving surface of the light receiving head may be provided toward the center of the subject.
That is, the projection head may be provided in the same direction as the projection direction at 90 ° lateral direction or at a position slightly away from the projection position with respect to the projection optical axis (projection direction optical axis) of the projection head.

The spectroscopic means connected from the light receiving head by an optical fiber is a combination of a light diffuser, a continuous variable interference filter (LV) and a photoelectric conversion element at the light output end of the optical fiber as in, for example, International Publication WO63 / 091676A1. Spectroscopy is performed using a packaged spectroscopic sensor unit having a sealed structure, and the spectroscopic spectrum data is output to the arithmetic processing means. Moreover, as a spectroscopic means, you may use the spectroscopic apparatus using the concave diffraction grating generally used as a spectroscope, and another well-known spectroscope and spectroscopic apparatus.
If the package type spectroscopic sensor unit is used, for example, even if an external impact force is applied, the optical axis and the wavelength are not shifted, the environment is not affected, and the device is compact and the device is configured compactly.

Arithmetic processing means for receiving spectral spectrum data includes an arithmetic unit, a setting unit, and a discrimination signal output unit. Spectral spectrum data is spectrum-analyzed by various analysis means such as multivariate analysis in the calculation unit, and optical data is digitized by statistical analysis.
In the setting unit, a sex discrimination threshold is set in advance. The male and female are discriminated by comparing the numerical value obtained by the arithmetic unit with the threshold value of the setting unit.
A female or male signal as a result of the discrimination is output from the output unit and discriminated and sorted as a display lamp or a sorting signal.

The analysis model for the multivariate analysis may be created for each cultivar variety, mulberry leaf growth and artificial feed growth, or a mixture of multiple varieties.
Further, disturbance factors such as the size of the subject and the body temperature of the subject can be corrected if they are taken into the analysis model as conditions of the subject when creating the analysis model.

The setting unit is configured to be able to input and set a threshold value for discriminating the sex of the subject in advance.
The threshold value may be set for each breed of mulberry leaves, artificial feed, or persimmon varieties, but preferably an analysis model is created so that males and females can be distinguished regardless of breeding conditions and breeds. So that the threshold can be set.

  In the setting part, not only one threshold value for discriminating females and males, but also a gray zone as an intermediate zone between them, the boundary value between females and gray zones, the boundary value between males and gray zones, that is, both female and male are clearly shown. An upper limit value and a lower limit value of gray zones that should not be set are set in advance, and those determined as gray zones may be configured to output and classify a signal that distinguishes females and males as poor sexually characteristic defects. .

The method and device for sorting cocoons based on female or male discrimination signals are: a table type in which an inspector supplies and examines specimens one by one; automatically feeds cocoons from the supply chute to the transfer means to inspect the transfer process. Widely used up to equipment that automatically tests on-line at the location.
Moreover, as a transfer means, a chute type that slides down an inclined surface, a conveyor type that travels and rotates endlessly, a rotary table type that transfers to a rotary table, or a two-position switching type that slides a receiving surface to an inspection position, etc. A known mechanism can be used.

  The receiving surface on which the subject of the transfer means is placed is configured such that a ridge having a shape close to a spindle shape is transferred to the inspection position with the length (height) direction or the body diameter direction being a fixed direction. That is, in order to bring the living cocoon having a spindle shape into a stable state at the inspection position, it is preferable to form the mounting surface in a groove shape, a concave surface or a cylindrical shape.

  Since the sputum that is the subject is a living body and is susceptible to mold and germs, hygiene and cleanliness are important. Therefore, the transporting means, the supplying means, the light projecting means, the light receiving means, etc. that are in contact with or close to the subject. Each part of the apparatus is configured so that it can be easily washed with water or alcohol.

  In addition, an apparatus used for mass processing that has to process tens of thousands of heads per day is provided with an automatic alignment supply device such as a parts feeder so that the subject is automatically aligned and supplied to the transfer means. Automatically discriminate between males and females and sort them.

  The cocoon is the original species for obtaining the hybrid (F1), and it is important that the varieties are separated and managed at the stage of the cocoon. For this reason, the apparatus for discriminating between males and females is configured so that, for each product type, the mechanical device is stopped, and it is checked and confirmed that no one animal remains, and then switched to the next product type to discriminate between males and females.

Hereinafter, a first embodiment of the present invention will be described in detail with reference to FIGS.
FIG. 1 is an explanatory diagram showing the overall configuration outline, FIG. 2 is an original spectrum diagram, and FIG. 3 is a regression plot diagram of the analysis results.

  In FIG. 1, reference numeral 100 denotes a transfer means, which transfers the eyelids S of the subject one by one to the examination position 1. This transfer means 100 transfers the kite S one by one so as to pass through or pass through the inspection position 1, and another embodiment is a cylindrical shape inclined so as to slide down naturally as shown in FIG. Various transfer forms such as those which are provided with an alignment transfer body 101 and flow down under their own weight, or as shown in FIG. 5, such as a rotary table type, a transfer conveyor type with a saucer, a belt conveyor type, and a slide transfer device (all not shown) The transfer means is used. In the simplest case, it includes placing it by hand.

Reference numeral 11 denotes light projecting means, and it is preferable to use a mini halogen lamp having a light projecting range of a halogen lamp 12 as a light projecting light source whose size is approximately the size of the body of the lamp S. A lens (not shown) may be combined to form a light projecting range so that the light beam is narrowed down to the size of the body of the rod S and projected. If it is difficult to place the light projecting lamp 12 in the immediate vicinity of the eyelid S of the subject, a light projecting fiber is provided in front of the halogen lamp 12 so that the light exit of the optical fiber is close to the body of the eyelid S, and the light is projected. You may comprise so that it may do (not shown).
Since the halogen lamp of the light projecting means 11 becomes hot and hot, it is preferable to make it air-cooled with a blower toward the lamp mounting portion. Further, the light projecting means 11 may be provided in combination with a plurality of light emitting diodes that output light beams of a plurality of specific wavelength bands. That is, any light source can be used as long as it can obtain light having a wavelength continuously varied by 1 to 2 nm in a wavelength range of 600 nm to 1000 nm.

A light receiving unit 20 is configured to guide transmitted light or diffused reflected light coming out of the body from the eyelid S at the inspection position to the spectroscopic unit 30 through the light receiving fiber 21.
The light receiving surface 22 of the light receiving fiber 21 is provided close to the outer peripheral surface of the bag S in order to allow weak light coming out from the body of the bag S to enter.
If the light receiving surface 22 of the optical fiber 21 is directly circumscribed by the ridge S, disturbance light that is not transmitted light does not enter, so that there is an advantage that noise is reduced.
Further, when the light projecting means 11 is used in combination with a light projecting fiber, the light projecting port and the light receiving surface of the light receiving fiber 21 are used to project and receive light by sandwiching the body of the collar S from both sides. Therefore, it can be determined without being influenced by disturbance light.

The transmitted light entering from the light receiving surface 22 is guided to the spectroscopic means 30 through the light receiving fiber 21. The spectroscopic means 30 splits the incident light beam for each wavelength, causes the split light beam to enter the light receiving surface of the photoelectric conversion element, and outputs an output corresponding to the wavelength. In the embodiment of the present invention, the small package spectral sensor unit 31 disclosed in WO03 / 091676A1 is used as the spectroscopic means 30. The small package spectroscopic sensor unit 31 is provided with a light diffuser 32 on the light output end face 23 of the light receiving fiber 21 and a continuous variable interference filter (LV) 33 on the light output side, followed by the photoelectric conversion element 34. The light receiving surface is provided and sealed in an airtight state in the package.
The photoelectric conversion element 34 outputs the spectral spectrum data of the light incident from the light receiving surface 22 of the light receiving fiber 21, A / D converts the output, and outputs it to the arithmetic processing means 40.

The arithmetic processing unit 40 performs spectrum analysis on the data by multivariate analysis in the arithmetic unit 41, and digitizes optical data in a specific wavelength band by predetermined statistical analysis. This numerical value is compared with a threshold value set in advance in the setting unit 42 to determine whether it is female or male, and is sorted from the output unit 43 as a female or male or neutral intermediate “?” As a result of the determination. Signals 44, 45 and 46 are output.
The multivariate analysis model used in the calculation unit 41 is preferably analyzed using a calibration curve created for each condition such as cultivar variety, mulberry leaf breeding or artificial breeding.
Further, the threshold value is similarly determined by changing the set value for each kind and breeding condition.
Reference numeral 47 denotes display means for displaying analysis data such as a split original spectrum and a graph of secondary differential values.

  FIG. 2 is an original spectrum diagram of the spectrum data 35 obtained from the spectroscopic means 30 for the variety G grown in mulberry leaves. In this figure, 351 is a female original spectrum and 352 is a male original spectrum. FIG. 3 is a regression plot diagram of multivariate analysis showing the result of sex determination by digitizing the spectrum data of FIG. 3 and comparing the numerical value with the threshold value of the setting unit. If the threshold is set to 1.5, it can be seen that the female and male are clearly separated.

48 is an apparatus for measuring the size of the heel S, and measures the size of the body of the heel S at the inspection position 1 or in the previous process. The size measuring device 48 includes a case where the size of the body is measured using a measuring camera and a case where the weight is measured using an electronic balance provided in association with the transfer means 100. A measurement signal measured by the size measuring device 48 is sent to the arithmetic processing means 40.
Further, it is preferable to provide a non-contact temperature measuring device (not shown) together with the size measuring device 48 so as to send the body temperature signal of the subject's eyelid S to the arithmetic processing means 40.
This magnitude measurement signal and body temperature signal can be reliably discriminated by introducing them into the analysis model as a disturbance element and statistically analyzing them.

  When discriminating between a female and a male, as in other organisms, the middle or neutral of a male or female is not necessarily said to be complete, so this neutral should be classified as “?”. Since the sorting signal 46 is provided in the above, reliable sorting is performed.

  Next, FIG. 4 showing a second embodiment of the present invention will be described. FIG. 4 uses an alignment transfer body 101 that is tilted so that the eyelids of the subject are aligned and naturally slide down as means for transferring the eyelids S of the apparatus for discrimination. In this example, an automatic aligning and supplying device 102 that supplies the ridge S toward the upper supply port of the cylindrical alignment transfer body 101 having a cylindrical shape or a partially opened upper portion is provided. This automatic alignment and supply device 102 uses an electromagnetic parts feeder or the like, and uses an apparatus formed so that a large number of groups of groups are aligned and supplied one by one.

A measuring stopper 103 is provided at the lower end of the alignment transfer body 101 to temporarily stop the specimen cage S that is slid down and bundled in the alignment transfer body 101. A light projecting hole and a light receiving hole are provided in a position corresponding to the body of the rod S temporarily stopped, that is, a side wall of the inspection position, and the light projecting lamp 12 of the light projecting means 11 and the optical fiber 21 of the light receiving means 20 are provided in each hole. The light receiving surface 22 is provided, and a light beam is projected onto the eyelid S received by the measurement stopper 103, and the transmitted light transmitted through the housing is received by the attendance surface 22.
The measurement stopper 103 is operated using a rotary solenoid, other driving devices, and various actuators. A cushion material is provided on the surface of the stopper 103 on which the collar S comes into contact to absorb the impact of the collar S that slides down, and is stopped so as not to be damaged.
Reception of transmitted light is performed during this stop. Further, it is preferable to provide a light shielding wall 104 around the ridge S received by the measurement stopper 103 so that disturbance light does not enter. That is, this portion is preferably formed in a tube shape through which the heel S is slid down.

Reference numeral 105 denotes a timing stopper, which is a stopper for separating the soot S received by the measurement stopper 103 so that the next soot does not continue during measurement.
When the timing stopper 105 is being measured by a pass sensor (not shown) of the saddle S provided in a part of the alignment transfer body 101 and a calculation unit connected by the light receiving means 20 and the optical fiber 21, the next stop S Is temporarily stopped so as not to enter the inspection position.
The operation device of the stopper 105 is configured to be operated using the same drive device and actuator as the measurement stopper 103.

  The hooks S are separated from the automatic alignment and supply device 102 one by one and supplied to the alignment transfer body 101. After passing through the timing stopper 105, the measuring stopper 103 receives the hook S, and the light projecting lamp 12 of the light projecting means 11 sets the hook S. Project light rays toward the body. Then, the transmitted light transmitted through the body of the bag S is received by the light receiving surface 22 of the light receiving means 20, guided to the spectral means (not shown) through the light receiving fiber 21, and spectrally separated in the same manner as in FIG. 1 of the first embodiment. The spectrum data 35 is analyzed by the arithmetic processing means 40, the sexes are discriminated, and sorting signals 44, 45 and 46 are output from the output unit 43. That is, the arithmetic processing means 40 is the same as in the first embodiment.

  Reference numerals 431 and 432 denote sorting devices, which include a female separator 431 and a male separator 432. Based on the discrimination results, the sorting signals 44, 45, and 46 output from the output unit 43 of the arithmetic processing means 40 are used to sort the bags into predetermined positions. If the measurement stopper 103 is opened after completion of the discrimination, and the rod S coming out from the lower end exit of the alignment transfer body 101 of the transfer means is a female signal 44, the female separator 431 is actuated to sort into the female section, and the male signal 45 If so, the male separator 432 is activated and sorted into the male area. If the neutral “?” Signal 46 is neither female nor male, the female separator 431 and the male separator 432 are not activated and are placed in the neutral “?” Area. Of course, the structures of the sorting devices 431 and 432 may be different from those shown in the drawings. That is, the mechanism for sorting the males and females may be configured in association with the structure of the transfer means.

  The shape of the alignment transfer body 101 may be a cylindrical tube shape or a U-shaped or V-grooved shape as long as the spindle-shaped ridges S slide down one by one in a fixed posture. . Other chute types may also be used. Since the kite S is alive and may move a little, it is preferable to use a guide groove (U groove, V groove) formed so as to go straight in the traveling direction.

  FIG. 4 shows the light projecting lamp 12 and the light receiving surface 22 of the light receiving means 20 provided on the lower and upper surfaces of the alignment transfer body 101. The light projecting lamp 12 and the light receiving surface 22 are shown in FIG. Thus, it may be provided on the left and right side surfaces in the transfer direction. In addition, since the light projecting optical axis and the light receiving surface of the light projecting lamp 12 need only be directed to the center of the body of the kite S (subject), the light projecting lamp 12 and the light receiving surface 22 need not be provided facing each other. Good. In other words, if the light projecting optical axis and the light receiving surface are provided toward the trunk center direction of the heel S, the projected light rays are diffusely reflected inside the trunk and transmitted light is emitted outside the body in various directions. The diffused and transmitted light is configured to be detected by the light receiving surface.

  Next, FIG. 5 showing a third embodiment of the present invention will be described. In FIG. 5, a rotating body 106 is used as a means for transferring the basket S (subject) of the apparatus to be discriminated, and a plurality of receiving trays 107 are provided on the rotating body 106 at equal intervals. Mount and rotate horizontally. The tray 107 rotates while maintaining the receiving posture so that the eyelid of the subject does not fall from the position where the eyelid S is supplied by the automatic alignment and supply device 102 to the examination position 1. This rotation may be either an intermittent rotation type that rotates in a constant step angle for each 1071 trays or a continuous rotation type. The continuous rotation type preferably rotates slowly.

  The construction of the light projecting lamp 12 and the light receiving means 20 at the inspection position 1 is provided similar to the first embodiment of FIG. Reference numeral 108 denotes a sorting unit, which operates a tray tilting / discharging device (not shown) on the basis of the discrimination result based on the sorting signals 44, 45, 46 output from the output unit 43 of the arithmetic processing means 40, and puts the bowl S on the tray. Sort and discharge to a predetermined position. This rotary (not shown) tray tilting and discharging apparatus uses various mechanisms in connection with the structure of the tray 107. For example, a tray such as a fruit weight sorter that has been practically used for a long time and its tilting and discharging mechanism are used. Used.

The upper surface of the tray 107 is formed with a guide groove (U-groove V-groove) so that the subject's wrinkle maintains a constant posture.
Since the mechanism for providing the projection lamp 12 and the light receiving surface 22 of the light receiving means 20 for the ridge S on the receiving tray 107 at the inspection position 1 is the same as that of the first embodiment, the description of the following configuration is omitted.

  In the first embodiment, the second embodiment, and the third embodiment, a light beam is projected onto the eyelid S at the examination position, and the transmitted light emitted from the inside of the eyelid is detected. It is preferable to cover the surroundings so that disturbing light does not enter the light receiving surface of the light receiving means, or to shield the light by providing a light shielding member.

  Thus, the method and apparatus for discriminating male and female moths according to the present invention provide a short period of time (7 to 10 days) from when a cocoon is formed until it becomes a cocoon in order to obtain good quality varieties in the sericulture industry. ) The discriminating work that used a large number of human hands to discriminate a large number of salmon with a daily volume of tens of thousands of heads is now possible to discriminate using a mechanical device according to this invention. Widely used.

  Since the apparatus of the present invention projects light rays onto the cocoon and spectroscopically analyzes the transmitted light, it is used to discriminate between males and females without damaging the living moths.

  In addition, the device of the present invention can be used to discriminate wrinkles by preventing the growth of molds and germs because the portion in contact with the wrinkles can be washed with alcohol or water.

Explanatory diagram showing the overall configuration overview Original spectrum of transmitted light from the enclosure A plot of the analyzed light data compared to the threshold Explanatory drawing which provided the alignment transfer body inclined in the transfer means Explanatory drawing of a rotary type using a rotating body as a transfer means

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 ... Inspection position 11 ... Projection means 12 ... Projection lamp 13 ... Projection hood 100 ... Transfer means 101 ... Alignment transfer Body 102 ... Automatic alignment supply device 103 ... Measurement stopper 104 ... Shading wall 105 ... Timing stopper 106 ... Rotating body 107 ... Receptacle 108... Sorting unit 20... Light receiving means 21... Light receiving fiber 22... Light receiving surface 23.・ ・ ・ ・ ・ Spectral sensor unit 32 ・ ・ ・ Light diffuser 33 ・ ・ ・ Continuous variable interference filter 34 ・ ・ ・ Photoelectric conversion element 35 ・ ・ ・ Spectral spectrum data 40 ・ ・ ・ ・・ Calculation processing means 41 ... Calculation unit 42 ... Setting unit 43 ... Output section 431 ... Female separator 432 ... Male separator 44, 45, 46 ... Sorting signal 47 ... Display means 48 ... Size measuring device S ... 蚕蛹 (subject)

Claims (9)

  The living body of the rod taken out from the cage is used as a subject, light is projected onto the subject using the light projecting means, the transmitted light from the cage is received using the light receiving head of the light receiving means, and the received light is spectrally separated. The spectrum is analyzed by analyzing the spectral spectrum, digitizing the optical data of a specific wavelength band, and determining whether it is female or male according to a preset threshold to separate males and females. A method for discriminating between male and female moths, characterized by outputting and discriminating.   The subject is supplied to the examination position one by one by a supply means, the light beam is projected onto the subject at the examination position, the transmitted light from the subject is received and subjected to spectral analysis, and the male and female are discriminated. The method for discriminating between male and female moths according to claim 1.   The subject is transported separately by the transport means, and a light beam is projected onto the subject being transported, and the transmitted light from the subject is received online for spectroscopic analysis to discriminate between males and females. 2. The method for discriminating male and female of a spider according to claim 1, wherein female and male are sorted according to the signal.   Using a living body of a rod as a subject, a light projecting means for projecting a light beam onto the subject, a light receiving means for receiving transmitted light from the subject, a spectrum of the received light, and a photoelectric conversion of the spectral spectrum and output A spectroscopic means for analyzing the spectroscopic spectrum by various analyzes such as multivariate analysis, and a numerical value obtained by the arithmetic unit for digitizing optical data, a setting unit for presetting a sex discrimination threshold, and the arithmetic unit A device for discriminating between male and female moths, comprising arithmetic processing means having an output unit for discriminating between males and females and outputting either female or male signals.   An alignment transfer body that is inclined so that the subject naturally slides down is provided, and a light projecting part and a light receiving part are provided at the tip thereof as an inspection position, and the eyelid that naturally slides down in the cylinder is continuously or temporarily stopped. The apparatus for discriminating between males and females according to claim 4, wherein the male and female are inspected and discriminated and sorted and sorted.   Inching or continuous transfer means for transferring the subject in a predetermined posture, a light projection means for projecting a projected light beam onto the subject on the transfer means, with a predetermined position in the transfer step as an inspection position, and the inspection position Thus, a light receiving means for receiving transmitted light from a direction different from the light projecting means from a direction different from the size of the outer shape of the subject is provided, and the received light is spectrally analyzed to discriminate between males and females. The apparatus for discriminating between male and female moths according to claim 4, further comprising an arithmetic processing unit that outputs such a sorting signal.   The transfer means uses transfer means that aligns and conveys the subject in a single row in a predetermined posture. When the subject travels through the examination position, it determines the received light in a non-stop manner with the timing of the subject at the examination position. The apparatus for discriminating between male and female moths according to claim 4 or 5, characterized in that the apparatus is configured to continuously sort and sort.   6. The apparatus for discriminating male and female moths according to claim 4 or 5, wherein the transfer means is configured to project and receive light on a subject using a tray on which the subject is placed in a predetermined posture. .   The light receiving means is configured to include an in / out mechanism for bringing the light receiving port close to or circumscribed to the subject when the subject is temporarily stopped at the examination position, and configured to receive the transmitted light in contact with the subject. The apparatus for discriminating between male and female moths according to claim 7.

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