CN106188277A - A kind of blood-eating hirudo adult feeds and the sustainable extracting method of hirudin and special equipment - Google Patents
A kind of blood-eating hirudo adult feeds and the sustainable extracting method of hirudin and special equipment Download PDFInfo
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- CN106188277A CN106188277A CN201610752855.9A CN201610752855A CN106188277A CN 106188277 A CN106188277 A CN 106188277A CN 201610752855 A CN201610752855 A CN 201610752855A CN 106188277 A CN106188277 A CN 106188277A
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- 241000237903 Hirudo Species 0.000 title claims abstract description 63
- 102000007625 Hirudins Human genes 0.000 title claims abstract description 50
- 108010007267 Hirudins Proteins 0.000 title claims abstract description 50
- WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 title claims abstract description 50
- 229940006607 hirudin Drugs 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000002895 emetic Substances 0.000 claims abstract description 17
- 230000006698 induction Effects 0.000 claims abstract description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 11
- 239000000284 extract Substances 0.000 claims abstract description 9
- 230000036528 appetite Effects 0.000 claims abstract description 7
- 235000019789 appetite Nutrition 0.000 claims abstract description 7
- 230000005855 radiation Effects 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 239000007921 spray Substances 0.000 claims abstract description 4
- 239000000287 crude extract Substances 0.000 claims description 13
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 12
- 239000003381 stabilizer Substances 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 8
- 230000007613 environmental effect Effects 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 229920001661 Chitosan Polymers 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 6
- 239000008107 starch Substances 0.000 claims description 6
- 235000019698 starch Nutrition 0.000 claims description 6
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 6
- 229960001763 zinc sulfate Drugs 0.000 claims description 6
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 239000004744 fabric Substances 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000002893 slag Substances 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 3
- 108090000190 Thrombin Proteins 0.000 description 7
- 229960004072 thrombin Drugs 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 4
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 3
- 241000243684 Lumbricus Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229960002897 heparin Drugs 0.000 description 3
- 229920000669 heparin Polymers 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 210000001835 viscera Anatomy 0.000 description 2
- 102000004411 Antithrombin III Human genes 0.000 description 1
- 108090000935 Antithrombin III Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000545744 Hirudinea Species 0.000 description 1
- 241000146385 Hirudo nipponia Species 0.000 description 1
- 241000521257 Hydrops Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 229960005348 antithrombin iii Drugs 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- YGANSGVIUGARFR-UHFFFAOYSA-N dipotassium dioxosilane oxo(oxoalumanyloxy)alumane oxygen(2-) Chemical compound [O--].[K+].[K+].O=[Si]=O.O=[Al]O[Al]=O YGANSGVIUGARFR-UHFFFAOYSA-N 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000003328 fibroblastic effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229910052627 muscovite Inorganic materials 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 210000003079 salivary gland Anatomy 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 210000003606 umbilical vein Anatomy 0.000 description 1
- IPCXNCATNBAPKW-UHFFFAOYSA-N zinc;hydrate Chemical compound O.[Zn] IPCXNCATNBAPKW-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Animal Behavior & Ethology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a kind of blood-eating hirudo adult to feed and the sustainable extracting method of hirudin, mainly comprise the following steps: 1) induce liquid to blood-eating hirudo fed to appetite, and again clean with the normal saline cleaning of 0.6 0.8%；2) emetic liquid is added according to the ratio of blood-eating hirudo weight with emetic liquid weight ratio 1:1；3) blood-eating hirudo is carried out ultraviolet light radiation-induced 23 times, each 10 15min, each induction interval 10 15min；6), after radiation-induced terminates, clean blood-eating hirudo with the normal saline of spray 0.6 0.8%, and collect rinse water liquid as hirudin extracting solution；A kind of method that the invention provides sustainable offer hirudin, has saved the resource of blood-eating hirudo, and what the present invention provided simultaneously extracts emetic can increase the single yield of hirudin, has significant economic benefit.
Description
Technical field
The present invention relates to the cultural technique of a kind of Chinese medicine animal drug, a kind of blood-eating hirudo adult is raised
Feed and the sustainable extracting method of hirudin and special equipment.
Technical background
Blood-eating hirudo (formal name used at school: Hirudo nipponia) be Yi Zhi section doctor trematodiasis belong to animal, popular name hirudo nipponica, Hirudo,
Line Hirudo, Hirudo, Japan doctor Hirudo.Be distributed in the Far East Area Japanese, Muscovite, Mongolia and China's Mainland North gets northeast
Each province, the Inner Mongol, west to Sichuan, Gansu, south reach the ground such as Guangdong, typically inhabit paddy field and the irrigation canals and ditches communicated therewith, pond
Most with quantity in hydrops in marsh and particularly throughout the year or the waterlogged plot of impeded drainage and field heart.
Hirudin (Hirudin) is to have extracted various active in Hirudo (Leech) or blood-eating hirudo and salivary gland thereof
A kind of composition that in point, activity is the most notable and most studied, the small protein that it is made up of 65-66 aminoacid
(polypeptide).Hirudo contains abundant hirudin, and hirudin has extremely strong inhibitory action to thrombin, is to be found up to now
The natural specific inhibitor of strong thrombin.Animal experiment shows with clinical research, hirudin energy Effective Anti blood coagulation, antithrombotic shape
Become, and stop the blood stasis phenomenon further such as thrombin activation and platelet response of catalyzed by thrombin.Additionally, it can also press down
Fibroblastic propagation of thrombin induction processed and the stimulation of thrombin Human Umbilical Vein Endothelial Cells.Compared with heparin, its not only consumption
Few, will not cause bleeding, also not dependent on endogenous co-factor；Heparin then has the danger caused bleeding, at diffusivity blood
In the pathogenic process of intravascular coagulation, antithrombin Ⅲ often reduces, and this will limit the curative effect of heparin, uses Hirudo to have preferably
Effect.The method proposing hirudin at present from blood-eating hirudo is directly to be put to death by blood-eating hirudo, directly extracts in polypide；Need
Want substantial amounts of Hirudo adult resource.
Summary of the invention
The invention provides a kind of blood-eating hirudo adult for solution the problems referred to above to feed and the sustainable extracting method of hirudin,
Comprise the following steps:
1) under 25-29 DEG C of environmental condition, adult blood-eating hirudo is carried out concentration and feeds, after feeding end, clear up feedstuff
Residue；
2) blood-eating hirudo after feeding was 22-25 DEG C of fasting 15-20 days；
3) under 25-29 DEG C of environmental condition, concentrate the blood-eating hirudo after fasting, clean with the normal saline of 0.6-0.8%
After, blood-eating hirudo is placed in container, induces liquid to blood-eating hirudo fed to appetite, and clean again with the normal saline of 0.6-0.8%
Clean；
4) it is formulated with zinc sulfate, acetic acid and the emetic liquid of water composition, blood-eating hirudo is laid in special container, according to day
This doctor trematodiasis weight adds emetic liquid with the ratio of emetic liquid weight ratio 1:1；
5) blood-eating hirudo is carried out ultraviolet light radiation-induced 2-3 time, each 10-15min, each induction interval 10-
15min；
6), after radiation-induced terminates, clean blood-eating hirudo with the normal saline of spray 0.6-0.8%, and collect rinse water liquid
As hirudin extracting solution；
7) after hirudin extracting solution adds stabilizer, it is spray-dried, obtains hirudin crude extract；
8), after the blood-eating hirudo after cleaning continues to raise 1 month, step 1 is repeated)-7) persistently extract hirudin.
Step 2) described in induction liquid be fresh and alive Monopteri albi extract and glucose mixing aqueous solution.
Stabilizer described in step 7 be the weight ratio of the mixture of starch and chitosan, starch and chitosan be 10:2.
A kind of special equipment of the blood-eating hirudo sustainable extraction of adult hirudin, described special equipment by urceolus, inner core and
Ultraviolet source is constituted；
Wherein, urceolus is box like structure or the barrel-like structure of upper opening, urceolus be provided above bracing frame urceolus
Bottom is provided with the valve for draining；Inner core is arranged at the inner side of urceolus, and inner core is box like structure or the tubbiness of upper opening
Structure, the bottom of inner core (2) is that filtering bottom board is constituted, and filtering bottom board is sieve plate or the screen cloth of aperture 20-100 mesh, and inner core connects
It is connected to hook；Outer light source is arranged on urceolus and the top of inner core upper opening.
The method have the benefit that: a kind of method that sustainable offer hirudin is provided, save
The resource of blood-eating hirudo, what the present invention provided simultaneously extracts emetic can increase the single yield of hirudin, has significantly
Economic benefit.
Figure of description
The structural representation of inner core mentioned by Fig. 1 special equipment；
Fig. 2 special equipment puts down the structural representation of inner core；
1 urceolus, 1-1 bracing frame, 1-2 valve, 2 inner cores, 2-1 filtering bottom board, 2-2 links up with；3 ultraviolet sources.
Detailed description of the invention
The structure of embodiment 1 special equipment and occupation mode
For the special equipment of the blood-eating hirudo sustainable extraction of adult hirudin, by urceolus 1, inner core 2 and ultraviolet source 3 structure
Become；Wherein, urceolus 1 is box like structure or the barrel-like structure of upper opening, urceolus 1 be provided above bracing frame 1-1, urceolus 1
Bottom be provided with the valve 1-2 for draining；Inner core 2 is arranged at the inner side of urceolus 1, and inner core 2 is the box like knot of upper opening
Structure or barrel-like structure, the bottom of inner core 2 is that filtering bottom board 2-1 is constituted, and filtering bottom board 2-1 is sieve plate or the sieve of aperture 20-100 mesh
Net, inner core 2 connects and has hook 2-2；Outer light source 3 is arranged on urceolus 1 and the top of inner core 2 upper opening.
When extracting hirudin, the adult blood-eating hirudo after first just feeding, 22-25 DEG C of fasting 15 days, then uses specially
With equipment under 25-29 DEG C of environmental condition, extract hirudin；Specifically comprise the following steps that
1., after cleaning with the normal saline of 0.7%, blood-eating hirudo is placed in inner core 2, inner core 2 is placed on urceolus 1
Bottom, the amount that blood-eating hirudo is placed is the inner core 2 every 0.01m of floor space2Place 600g to grow up Hirudo；
Induce liquid 2. to blood-eating hirudo fed to appetite Monopteri albi, then clean with the normal saline of 0.7%, drained by valve 1-2,
To without circulating water；
3. close valve 1-2, add emetic liquid according to the ratio of blood-eating hirudo weight with emetic liquid weight ratio 1:1；
4. blood-eating hirudo is carried out ultraviolet light radiation-induced 3 times, each 15min, each induction interval 15min；
5. extract inner core 2, on the bracing frame 1-1 hang on urceolus 1 by the hook 2-2 being connected on inner core 2, make inner core 2
Leave the liquid level bottom urceolus 1；The normal saline of spray 0.7% cleans blood-eating hirudo.
6. cleanout fluid is collected as hirudin extracting solution by valve 1-2.
7., after hirudin extracting solution adds stabilizer, it is spray-dried, obtains hirudin crude extract.
The different emetic impact on blood-eating hirudo hirudin extracted amount of embodiment 2
Weigh adult blood-eating hirudo 30kg, be divided into 3 groups, give entitled A group, B group and C group respectively, often organize 10kg；The most just
Adult blood-eating hirudo after feeding, 22-25 DEG C of fasting 15 days, then uses special equipment under 25-29 DEG C of environmental condition, joins
Hirudin is extracted according to the method described in embodiment 1.A group feeds by zinc sulfate, acetic acid and the emetic of water weight ratio 2:5:100；A
Group feeds by zinc sulfate, 95% ethanol and the emetic of water weight ratio 2:5:100；C group feeds zinc sulfate and water weight ratio 2:100
Emetic.After each group collects hirudin extracting solution, add the stabilizer that weight ratio is 10:2 composition of starch and chitosan
Stabilizer, addition is for often organizing 100g.Hirudin crude extract is obtained after being spray-dried immediately.Detect each group of Hirudo prepared
Element crude extract.Anticoagulation during the content of antithrombase is 1210AT-U/g, B group hirudin crude extract in A group hirudin crude extract
The content of enzyme is that in 1040AT-U/g, B group hirudin crude extract, the content of antithrombase is 870AT-U/g.
The impact of the induction liquid blood-eating hirudo hirudin extracted amount that embodiment 3 is different
(1) preparation induction liquid
Weighing fresh and alive Monopteri albi 1000g, stripping and slicing is pulverized, and puts in 5000ml water, and 100 mesh filter, and collects water liquid, and at water
Liquid adds 200g glucose, prepares Monopteri albi induction liquid；
Weighing animal livers 1000g, stripping and slicing is pulverized, and puts in 5000ml water, and 100 mesh filter, and collects water liquid, and at water
Liquid adds 200g glucose, prepares internal organs induction liquid；
Weighing fresh and alive Lumbricus 1000g, stripping and slicing is pulverized, and puts in 5000ml water, and 100 mesh filter, and collects water liquid, and at water
Liquid adds 200g glucose, prepares Lumbricus induction liquid；
(2) investigation of liquid is induced
Weigh adult blood-eating hirudo 30kg, be divided into 3 groups, give entitled I group, II group and III group respectively, often organize 10kg；First will
Adult blood-eating hirudo after feeding was 22-25 DEG C of fasting 15 days, and then employing special equipment is under 25-29 DEG C of environmental condition,
Hirudin is extracted with reference to the method described in embodiment 1.I group of fed to appetite Monopteri albi induction liquid, II group of fed to appetite internal organs induction liquid, III group of fed to appetite
Lumbricus induction liquid；It is respectively washed each group, and gets rid of water stain.
Each group feeds zinc sulfate, acetic acid and the emetic of water weight ratio 2:10:100 respectively, and each group is collected hirudin and extracted
After liquid, adding the stabilizer of the stabilizer that weight ratio is 10:2 composition of starch and chitosan, addition is for often organizing 100g.Immediately
Hirudin crude extract is obtained after spray drying.Detect each group of hirudin crude extract prepared.A group hirudin crude extract resists
The content of thrombin is that in 1130AT-U/g, B group hirudin crude extract, the content of antithrombase is 960AT-U/g, B group hirudin
In crude extract, the content of antithrombase is 730AT-U/g.
Claims (4)
1. a blood-eating hirudo adult feeds and the sustainable extracting method of hirudin, it is characterised in that: comprise the following steps:
1) under 25-29 DEG C of environmental condition, adult blood-eating hirudo is carried out concentration and feeds, clear up feedstuff after feeding end residual
Slag；
2) blood-eating hirudo after feeding was 22-25 DEG C of fasting 15-20 days；
3) under 25-29 DEG C of environmental condition, the blood-eating hirudo after fasting is concentrated, after cleaning with the normal saline of 0.6-0.8%, will
Blood-eating hirudo is placed in container, induces liquid to blood-eating hirudo fed to appetite, and again cleans with the normal saline cleaning of 0.6-0.8%；
4) it is formulated with zinc sulfate, acetic acid and the emetic liquid of water composition, blood-eating hirudo is laid in special container, according to Japan
Doctor's trematodiasis weight adds emetic liquid with the ratio of emetic liquid weight ratio 1:1；
5) blood-eating hirudo is carried out ultraviolet light radiation-induced 2-3 time, each 10-15min, each induction interval 10-15min；
6), after radiation-induced terminates, clean blood-eating hirudo with the normal saline of spray 0.6-0.8%, and collect rinse water liquid conduct
Hirudin extracting solution；
7) after hirudin extracting solution adds stabilizer, it is spray-dried, obtains hirudin crude extract；
8), after the blood-eating hirudo after cleaning continues to raise 1 month, step 1)-7 is repeated) persistently extract hirudin.
Blood-eating hirudo adult the most according to claim 1 feeds and the sustainable extracting method of hirudin, it is characterised in that: step
Rapid 2) the induction liquid described in is fresh and alive Monopteri albi extract and the aqueous solution of glucose mixing.
Blood-eating hirudo adult the most according to claim 1 feeds and the sustainable extracting method of hirudin, it is characterised in that: step
Stabilizer described in rapid 7 be the weight ratio of the mixture of starch and chitosan, starch and chitosan be 10:2.
4. the special equipment of the blood-eating hirudo sustainable extraction of adult hirudin, it is characterised in that: described special equipment is by outward
Cylinder (1), inner core (2) and ultraviolet source (3) are constituted；
Wherein, urceolus (1) is box like structure or the barrel-like structure of upper opening, urceolus (1) be provided above bracing frame (1-1)
The bottom of urceolus (1) is provided with the valve (1-2) for draining；Inner core (2) is arranged at the inner side of urceolus (1), and inner core (2) is upper
The box like structure of side's opening or barrel-like structure, the bottom of inner core (2) is that filtering bottom board (2-1) is constituted, and filtering bottom board (2-1) is hole
The sieve plate of footpath 20-100 mesh or screen cloth, the upper connection of inner core (2) has hook (2-2)；Outer light source (3) is arranged on urceolus (1) and inner core
(2) top of upper opening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610752855.9A CN106188277A (en) | 2016-09-30 | 2016-09-30 | A kind of blood-eating hirudo adult feeds and the sustainable extracting method of hirudin and special equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610752855.9A CN106188277A (en) | 2016-09-30 | 2016-09-30 | A kind of blood-eating hirudo adult feeds and the sustainable extracting method of hirudin and special equipment |
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| Publication Number | Publication Date |
|---|---|
| CN106188277A true CN106188277A (en) | 2016-12-07 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106749638A (en) * | 2016-12-29 | 2017-05-31 | 艾普博科技（天津）有限公司 | A kind of hirudin extraction element |
| CN107661357A (en) * | 2017-11-16 | 2018-02-06 | 广西远程水蛭养殖有限公司 | Leech medicinal liquor preparation method |
| CN109527270A (en) * | 2018-12-10 | 2019-03-29 | 苏州大学 | Leech specific nutrient induces the extracting method of liquid and natural hirudin |
| CN109937927A (en) * | 2017-12-21 | 2019-06-28 | 南京农业大学 | A kind of method of quick raising leech antithrombase content |
| CN110623275A (en) * | 2019-09-18 | 2019-12-31 | 周维海 | Composite amino acid gilt-edged leech polypeptide food with anticoagulant and antithrombotic effects and preparation method thereof |
| CN115067247A (en) * | 2022-08-03 | 2022-09-20 | 重庆志微生物技术有限公司 | Breeding device and breeding method for increasing output of hirudo nipponia |
| CN116496387A (en) * | 2023-06-21 | 2023-07-28 | 云南云蛭康生物科技有限公司 | Extraction method of hirudin |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1423929A (en) * | 2003-01-15 | 2003-06-18 | 周维海 | Culture of hiruto and extraction of hirutin |
| CN102952189A (en) * | 2012-10-19 | 2013-03-06 | 旌德县振亚水产养殖有限公司 | Hirudin extraction bottle and hirudin extraction method |
| CN102964446A (en) * | 2012-11-28 | 2013-03-13 | 周维官 | Method for repeatedly extracting natural hirudin from live vampire leech |
| CN104926937A (en) * | 2015-06-19 | 2015-09-23 | 广西复鑫益生物科技有限公司平南分公司 | Method for extracting hirudin from leech saliva |
| CN105693851A (en) * | 2016-04-16 | 2016-06-22 | 徐顺青 | Hirudin extracting method |
-
2016
- 2016-09-30 CN CN201610752855.9A patent/CN106188277A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1423929A (en) * | 2003-01-15 | 2003-06-18 | 周维海 | Culture of hiruto and extraction of hirutin |
| CN102952189A (en) * | 2012-10-19 | 2013-03-06 | 旌德县振亚水产养殖有限公司 | Hirudin extraction bottle and hirudin extraction method |
| CN102964446A (en) * | 2012-11-28 | 2013-03-13 | 周维官 | Method for repeatedly extracting natural hirudin from live vampire leech |
| CN104926937A (en) * | 2015-06-19 | 2015-09-23 | 广西复鑫益生物科技有限公司平南分公司 | Method for extracting hirudin from leech saliva |
| CN105693851A (en) * | 2016-04-16 | 2016-06-22 | 徐顺青 | Hirudin extracting method |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106749638A (en) * | 2016-12-29 | 2017-05-31 | 艾普博科技（天津）有限公司 | A kind of hirudin extraction element |
| CN107661357A (en) * | 2017-11-16 | 2018-02-06 | 广西远程水蛭养殖有限公司 | Leech medicinal liquor preparation method |
| CN109937927A (en) * | 2017-12-21 | 2019-06-28 | 南京农业大学 | A kind of method of quick raising leech antithrombase content |
| CN109527270A (en) * | 2018-12-10 | 2019-03-29 | 苏州大学 | Leech specific nutrient induces the extracting method of liquid and natural hirudin |
| CN110623275A (en) * | 2019-09-18 | 2019-12-31 | 周维海 | Composite amino acid gilt-edged leech polypeptide food with anticoagulant and antithrombotic effects and preparation method thereof |
| CN115067247A (en) * | 2022-08-03 | 2022-09-20 | 重庆志微生物技术有限公司 | Breeding device and breeding method for increasing output of hirudo nipponia |
| CN116496387A (en) * | 2023-06-21 | 2023-07-28 | 云南云蛭康生物科技有限公司 | Extraction method of hirudin |
| CN116496387B (en) * | 2023-06-21 | 2023-09-12 | 云南云蛭康生物科技有限公司 | Extraction method of hirudin |
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