CN105111781B - A kind of melanic industrialized preparing process of cephalopod - Google Patents
A kind of melanic industrialized preparing process of cephalopod Download PDFInfo
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- CN105111781B CN105111781B CN201510616447.6A CN201510616447A CN105111781B CN 105111781 B CN105111781 B CN 105111781B CN 201510616447 A CN201510616447 A CN 201510616447A CN 105111781 B CN105111781 B CN 105111781B
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Abstract
The invention discloses a kind of melanic industrialized preparing process of cephalopod, including preparation and the melanic indudstrialized refining of cephalopod of cephalopod melanin semifinished product.Method is simple, and raw material sources are wide, and product purity is high, possesses significant economic and social benefit.
Description
Technical field
The invention belongs to marine product field of deep, and in particular to a kind of melanic industrialized production side of cephalopod
Method.
Background technology
Ink sepiellae seu sepiae is, by the material extracted in the ink sac of Sepiidae animal, to gain the name because of its color and source.By tiny black
The thick suspension that coloured particles are constituted, composition are probably the proteoglycan complex being connected on melanin, containing various
Active component, with higher medical value.
The content of the invention
It is an object of the invention to provide a kind of melanic industrialized preparing process of cephalopod, method is simple, former
Material source is wide, and product purity is high, possesses significant economic and social benefit.
For achieving the above object, the present invention is adopted the following technical scheme that：
A kind of melanic industrialized preparing process of cephalopod, including the preparation of cephalopod melanin semifinished product
With the melanic indudstrialized refining of cephalopod.
The preparation of cephalopod melanin semifinished product include from Sepiella maindroni live body extract melanin semifinished product,
From frost Sepiella maindroni in extract melanin semifinished product, from the ink-jet water of dredger extract melanin semifinished product, from work
Melanin semifinished product is extracted in industry cyclic culture backwater and extraction melanin is rough from cephalopod processing fent
Product.
From Sepiella maindroni live body or extracting melanin semifinished product from frost Sepiella maindroni includes following step
Suddenly：
（1）Sepiella maindroni ink sac is peeled off manually or mechanically；
（2）By the way of extruding or squeezing, the prepared Chinese ink in ink sac is extruded, fine straining again after gauze coarse filtration obtains fine straining liquid；
（3）By the ink sac of extrusion prepared Chinese ink, washed with warm water 3 times, discard tissue, collect filtrate；Using spiral-flow type depositor
Prepared Chinese ink in water is intercepted, with step（2）Fine straining liquid merge；
（3）Concentration, is dried, obtains cephalopod melanin semifinished product.
It is to intercept prepared Chinese ink in water using spiral-flow type depositor that melanin semifinished product is extracted from the ink-jet water of dredger, and Jing is dense
Contracting, is dried, obtains cephalopod melanin semifinished product.
Melanin semifinished product is extracted from industrialization cyclic culture backwater to comprise the following steps：
（1）Sepia in cultivation backwater is intercepted using spiral-flow type depositor；Using in arc sieve separation cultivation backwater
Solid particulate matter；Suspended particulate substance in cultivation backwater is intercepted using multidirectional jet submersible formula Airfloat pump；
（2）The prepared Chinese ink concentrate collected respectively, solid particulate matter and suspended particulate substance are merged into drying, siphonopods is obtained and is moved
Thing melanin semifinished product.
Melanin semifinished product is extracted from cephalopod processing fent to comprise the following steps：
（1）Cephalopod processing fent is thawed, machinery is blended, plus 50wt.% ethanol solution, stirring makes melanin
Dissolving, filters, and collects filtrate；
（2）Residue is extracted with 50wt.% ethanol solution again, collects filtrate；
（3）Combining step（1）（2）Filtrate, reclaim ethanol, obtain cephalopod melanin semifinished product.
The melanic indudstrialized refining of cephalopod is pH=9 ~ 12 and bottom to 50 DEG C using alkaline protease in room temperature
Thing concentration is 5 ~ 8h of enzymolysis under conditions of 1 ~ 5wt.%, filters, discards residue, filtrate is dried, and obtains highly purified siphonopods and moves
Thing melanin.
The melanic indudstrialized refining of cephalopod is the pH to 50 DEG C using lime water or sodium hydroxide solution in room temperature
5 ~ 8h of alkaline hydrolysis under the conditions of=9 ~ 12, filters, discards residue, filtrate is dried, obtain highly purified cephalopod melanin.
The melanic indudstrialized refining of cephalopod be the acetic acid or hydrochloric acid for adopting in room temperature to 50 DEG C, pH=1 ~ 4 condition
5 ~ 7h of lower alkaline hydrolysis, filters, discards residue, filtrate is dried, obtain highly purified cephalopod melanin.
The present invention remarkable advantage be：Method is simple, and raw material sources are wide, and product purity is high（More than 98%）, possess significantly
Economic and social benefit.
Specific embodiment
（One）It is prepared by cephalopod melanin semifinished product
1st, melanin semifinished product is prepared from the Sepiella maindroni of artificial cultivation
（1）Melanin semifinished product is prepared from Sepiella maindroni live body：Using the Man needleless of existing large-scale cultivation
Squid living body resource, efficiently peels off ink sac.The Sepiella maindroni that wild i.e. sea area is fished for easily by stress, ink-jet and it is dead
Die, cause prepared Chinese ink in the sepiellae seu sepiae product body fished for greatly reduce.At present can be with the Sepiella maindroni of scale artificial culture.
The Sepiella maindroni of artificial cultivation, because of domestication, substantially reduce stress ink-jet, from cultivation place such as sea area net cage, pond
Product is obtained after fishing for interior, it is more which is retained in prepared Chinese ink in sepiellae seu sepiae body.For the Man needleless crow of the artificial cultivation fished for
Crafty product, can take following technology to take ink.
1）Sepiella maindroni ink sac is peeled off manually or mechanically；
2）Ink sac is preserved：Ice preservation can typically be dissipated；It is big for quantity, need to wait for the long period person that takes prepared Chinese ink, can be with ice
Freeze and preserve；
3）Prepared Chinese ink is taken directly from ink sac：Extruding is squeezed, and extrudes prepared Chinese ink, fine straining again after gauze coarse filtration；
4）It is prepared by prepared Chinese ink concentrate：Prepared Chinese ink filtrate is dried in the shade or heat drying naturally, melanin crude product has both been obtained；
5）To increase operation rate, the ink sac after extrusion prepared Chinese ink can also be washed with water 3 times, tissue is discarded, filtrate is collected,
It is standby；Sepia in water is intercepted with spiral-flow type depositor, concentration is dried, obtains final product pitch-dark pigment semifinished product.
（2）Melanin semifinished product is prepared from frost siphonopods marine organisms
1）Thaw；
2）Sepiella maindroni ink sac is peeled off manually or mechanically；
3）Prepared Chinese ink is taken directly from ink sac：Warm softens ink sac, extrudes or squeezes, and extrudes prepared Chinese ink, smart again after gauze coarse filtration
Filter.By the ink sac after extrusion prepared Chinese ink, washed with warm water 3 times, discard tissue, collect filtrate, it is standby；Intercepted with spiral-flow type depositor
Sepia in water, concentration are dried, obtain final product melanin semifinished product；
4）It is prepared by prepared Chinese ink concentrate：Prepared Chinese ink filtrate is dried in the shade or heat drying naturally, melanin semifinished product has both been obtained；
5）For manually or mechanically peeling off during Sepiella maindroni ink sac containing Sepiella maindroni other internal organs groups
Knit, prepare by " 3, siphonopods processing fent thing extract " after collection.
(3) melanin semifinished product is prepared from the ink-jet water of dredger：Cryopreservation, it is standby；Cut with spiral-flow type depositor
Sepia in water intaking, concentration are dried, obtain final product.
2nd, melanin semifinished product is prepared from industrialization circulating water cultivation backwater：By spiral-flow type depositor, sieve bend, multidirectional
Jet submersible formula Airfloat pump is constituted, and intercepts sepia, the solid particulate matter separated in cultivation backwater, gas in cultivation backwater respectively
The floating suspended particulate substance intercepted in cultivation backwater.The prepared Chinese ink concentrate collected respectively, solid particulate matter, suspended particulate substance are merged
It is dried, obtains final product.
3rd, melanin semifinished product is prepared from siphonopods processing fent thing：Siphonopods processing fent thing is thawed, machine
Tool is blended, plus 50% ethanol solution, and stirring dissolves melanin, is filtered, and collects filtrate；Residue is extracted with 50% ethanol again, is collected
Filtrate.Merging filtrate, reclaims ethanol, has both obtained melanin semifinished product.
（Two）The melanic indudstrialized refining method of cephalopod
1st, as needed, it is room temperature to 50 DEG C in temperature with the alkaline protease of different proportion, pH value 9 ~ 12 and substrate are dense
Spend for 5 ~ 8h is digested under conditions of 1 ~ 5%, filter, discard residue, filtrate is dried, both different molecular weight high-purity
（98.5%）Melanin.
2nd, as needed, with the lime water of different proportion（Sodium hydroxide can also be used）Temperature be room temperature to 50 DEG C, pH value 9
5 ~ 8h of alkaline hydrolysis under the conditions of ~ 12, filters, discards residue, filtrate is dried, and both obtains the high-purity of different molecular weight（98.9%）It is black
Pigment.
3rd, as needed, with the acetic acid of different proportion（Hydrochloric acid can also be used）Temperature be room temperature to 50 DEG C, 1 ~ 4 condition of pH value
5 ~ 7h of lower alkaline hydrolysis, filters, discards residue, filtrate is dried, and both obtains the high-purity of different molecular weight（99.6%）Melanin.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (4)
1. the melanic industrialized preparing process of a kind of cephalopod, it is characterised in that：It is thick including cephalopod melanin
The preparation of product and the melanic indudstrialized refining of cephalopod；
The preparation of cephalopod melanin semifinished product include from Sepiella maindroni live body extract melanin semifinished product, from ice
Freeze Sepiella maindroni in extract melanin semifinished product, from the ink-jet water of dredger extract melanin semifinished product, from industrialization
Melanin semifinished product is extracted in cyclic culture backwater and melanin semifinished product is extracted from cephalopod processing fent；
（One）From Sepiella maindroni live body or extracting melanin semifinished product from frost Sepiella maindroni includes following step
Suddenly：
（1）Sepiella maindroni ink sac is peeled off manually or mechanically；
（2）By the way of extruding or squeezing, the prepared Chinese ink in ink sac is extruded, fine straining again after gauze coarse filtration obtains fine straining liquid；
（3）By the ink sac of extrusion prepared Chinese ink, washed with warm water 3 times, discard tissue, collect filtrate；Intercepted using spiral-flow type depositor
Prepared Chinese ink in water, with step（2）Fine straining liquid merge；
（4）Concentration, is dried, obtains cephalopod melanin semifinished product；
（Two）It is to intercept prepared Chinese ink in water using spiral-flow type depositor that melanin semifinished product is extracted from the ink-jet water of dredger, and Jing is dense
Contracting, is dried, obtains cephalopod melanin semifinished product；
（Three）Melanin semifinished product is extracted from industrialization cyclic culture backwater to comprise the following steps：
（1）Sepia in cultivation backwater is intercepted using spiral-flow type depositor；Consolidating in backwater is cultivated using arc sieve separation
Body particulate matter；Suspended particulate substance in cultivation backwater is intercepted using multidirectional jet submersible formula Airfloat pump；
（2）The prepared Chinese ink concentrate collected respectively, solid particulate matter and suspended particulate substance are merged into drying, cephalopod is obtained black
Pigment semifinished product；
（Four）Melanin semifinished product is extracted from cephalopod processing fent to comprise the following steps：
（1）Cephalopod processing fent is thawed, machinery is blended, plus 50wt.% ethanol solution, stirring dissolves melanin,
Filter, collect filtrate；
（2）Residue is extracted with 50wt.% ethanol solution again, collects filtrate；
（3）Combining step（1）（2）Filtrate, reclaim ethanol, obtain cephalopod melanin semifinished product.
2. method according to claim 1, it is characterised in that：The melanic indudstrialized refining of cephalopod is to adopt alkali
Property protease in room temperature to 50 DEG C, pH=9 ~ 12 and concentration of substrate are to digest 5 ~ 8h under conditions of 1 ~ 5wt.%, filter, discard residual
Slag, filtrate is dried, and obtains highly purified cephalopod melanin.
3. method according to claim 1, it is characterised in that：The melanic indudstrialized refining of cephalopod is to adopt stone
, in room temperature to 50 DEG C, 5 ~ 8h of alkaline hydrolysis under the conditions of pH=9 ~ 12 is filtered, is discarded residue, filtrate is done for buck or sodium hydroxide solution
It is dry, obtain highly purified cephalopod melanin.
4. method according to claim 1, it is characterised in that：The melanic indudstrialized refining of cephalopod is what is adopted
, in room temperature to 50 DEG C, 5 ~ 7h of acidolysis under the conditions of pH=1 ~ 4 is filtered, is discarded residue, filtrate is dried, obtain high-purity for acetic acid or hydrochloric acid
The cephalopod melanin of degree.
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| CN106009762A (en) * | 2016-05-20 | 2016-10-12 | 佛山市聚成生化技术研发有限公司 | Novel ink melanin extraction method |
| CN105875772A (en) * | 2016-06-15 | 2016-08-24 | 大连民族大学 | Cuttlefish juice sugarless soda biscuit and making method thereof |
| CN106189355B (en) * | 2016-08-11 | 2017-10-31 | 安徽阳光药业有限公司 | The method for extracting squid heath-function melanin |
| CN107195883A (en) * | 2017-05-31 | 2017-09-22 | 青岛大学 | A kind of preparation method of nitrogen-doped carbon nanosphere/molybdenum disulfide sodium ion composite nano materials |
| CN108795095B (en) * | 2017-11-02 | 2020-03-24 | 安发（福建）生物科技有限公司 | Drying method of marine animal melanin |
| CN108795092A (en) * | 2017-11-02 | 2018-11-13 | 安发（福建）生物科技有限公司 | A kind of preparation method of the pitch-dark pigment of marine animal |
| CN108795093A (en) * | 2017-11-02 | 2018-11-13 | 安发（福建）生物科技有限公司 | A kind of preparation of the pitch-dark pigment of marine animal solubility and analysis method |
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| JPS5771379A (en) * | 1980-10-22 | 1982-05-04 | Zenchin:Kk | Preparation of sliced raw cuttlefish seasoned with mustard |
| CN100487064C (en) * | 2006-12-22 | 2009-05-13 | 中国海洋大学 | Preparation process of squid ink black pigment and application thereof |
| JP2012207140A (en) * | 2011-03-30 | 2012-10-25 | Toyo Ink Sc Holdings Co Ltd | Cuttlefish ink dye aqueous dispersion, and method for manufacturing the same |
| CN102776238B (en) * | 2011-05-09 | 2015-03-18 | 浙江海洋学院 | Preparation method for natural melanin from squid ink |
| CN102499389B (en) * | 2011-10-24 | 2013-08-07 | 浙江大学 | Preparation method and use of soluble squid ink melanin |
| CN103848470A (en) * | 2012-12-06 | 2014-06-11 | 浙江海洋学院 | Method for clearing hexavalent chromium ions by squid melanin |
| CN104544321A (en) * | 2013-10-21 | 2015-04-29 | 大连市沙河口区中小微企业服务中心 | Soluble squid inkiness pigment |
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