CN105111781A - Industrial production method of cephalopod melanin - Google Patents
Industrial production method of cephalopod melanin Download PDFInfo
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- CN105111781A CN105111781A CN201510616447.6A CN201510616447A CN105111781A CN 105111781 A CN105111781 A CN 105111781A CN 201510616447 A CN201510616447 A CN 201510616447A CN 105111781 A CN105111781 A CN 105111781A
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Abstract
The invention discloses an industrial production method of cephalopod melanin. The industrial production method of the cephalopod melanin comprises the following steps of preparation of crude products of the cephalopod melanin; and industrial refining of the cephalopod melanin. The method is simple, sources of raw materials are wide, purity of products is high, and economic benefit and social benefit are obvious.
Description
Technical field
The invention belongs to sea-food field of deep, be specifically related to the melanic industrialized preparing process of a kind of cephalopod.
Background technology
Squid ink is by the material extracted in the ink sac of Sepiidae animal, gains the name because of its color and source.The thick suspension be made up of tiny black particle, composition may be the protein-polysaccharide mixture be connected on melanochrome, containing various active composition, has higher pharmaceutical use.
Summary of the invention
The object of the present invention is to provide the melanic industrialized preparing process of a kind of cephalopod, method is simple, and raw material sources are wide, and product purity is high, possesses significant economic and social benefit.
For achieving the above object, the present invention adopts following technical scheme:
The melanic industrialized preparing process of a kind of cephalopod, comprises preparation and the melanic indudstrialized refining of cephalopod of cephalopod melanochrome raw product.
The preparation of cephalopod melanochrome raw product comprises to extract melanochrome raw product, extracts melanochrome raw product from freezing Sepiella maindroni, extracts melanochrome raw product from the ink-jet water of dredger, extracts melanochrome raw product and extract melanochrome raw product from industrialization cyclic culture backwater from cephalopod processing fent from Sepiella maindroni live body.
From Sepiella maindroni live body or from freezing Sepiella maindroni, extract melanochrome raw product comprise the following steps:
(1) artificial or mechanically peel Sepiella maindroni ink sac;
(2) adopt the mode of extruding or squeezing, extruded by the prepared Chinese ink in ink sac, after gauze coarse filtration, essence filter again, obtains smart filtrate;
(3) will the ink sac of prepared Chinese ink be extruded, and wash 3 times with warm water, discard tissue, collect filtrate; Adopt whirlwind-type settling vessel to intercept prepared Chinese ink in water, merge with the smart filtrate of step (2);
(3) concentrated, dry, obtain cephalopod melanochrome raw product.
From the ink-jet water of dredger, extract melanochrome raw product is adopt whirlwind-type settling vessel to intercept prepared Chinese ink in water, through concentrated, dry, obtains cephalopod melanochrome raw product.
From industrialization cyclic culture backwater, extract melanochrome raw product comprise the following steps:
(1) whirlwind-type settling vessel is adopted to intercept the sepia cultivated in backwater; Sieve-bend screen is adopted to be separated the solid particulate matter cultivated in backwater; Multidirectional jet submersible formula Airfloat pump is adopted to intercept the suspended particulate cultivated in backwater;
(2) prepared Chinese ink enriched material, solid particulate matter and the suspended particulate collected respectively are merged dry, obtain cephalopod melanochrome raw product.
From cephalopod processing fent, extract melanochrome raw product comprise the following steps:
(1) thawed by cephalopod processing fent, machinery blends, and adds 50wt.% ethanolic soln, stirs and melanochrome is dissolved, and filters, and collects filtrate;
(2) residue extracts with 50wt.% ethanolic soln again, collects filtrate;
(3) filtrate of combining step (1) (2), reclaims ethanol, obtains cephalopod melanochrome raw product.
The melanic indudstrialized refining of cephalopod is for adopting Sumizyme MP at normal temperature to 50 DEG C, and pH=9 ~ 12 and concentration of substrate are enzymolysis 5 ~ 8h under the condition of 1 ~ 5wt.%, filter, discard residue, and filtrate is dry, obtain highly purified cephalopod melanochrome.
The melanic indudstrialized refining of cephalopod is for adopting liming or sodium hydroxide solution at normal temperature to 50 DEG C, and alkaline hydrolysis 5 ~ 8h under the condition of pH=9 ~ 12, filters, discard residue, and filtrate is dry, obtains highly purified cephalopod melanochrome.
The melanic indudstrialized refining of cephalopod be the acetic acid or hydrochloric acid that adopt at normal temperature to 50 DEG C, alkaline hydrolysis 5 ~ 7h under the condition of pH=1 ~ 4, filters, discards residue, and filtrate is dry, obtains highly purified cephalopod melanochrome.
Remarkable advantage of the present invention is: method is simple, and raw material sources are wide, and product purity high (being greater than 98%), possesses significant economic and social benefit.
Embodiment
(one) cephalopod melanochrome raw product preparation
1, melanochrome raw product is prepared from the Sepiella maindroni propagated artificially
(1) from Sepiella maindroni live body, prepare melanochrome raw product: the Sepiella maindroni live body resource utilizing existing large-scale cultivation, efficiently peel off ink sac.The Sepiella maindroni that wild i.e. sea area is fished for is subject to stress reaction, ink-jet and dead, causes prepared Chinese ink in the cuttlefish product body of fishing for greatly reduce.At present can the Sepiella maindroni of scale artificial culture.The Sepiella maindroni propagated artificially, because of domestication, greatly reducing stress ink-jet, from cultivation place as sea area net cage, pond and indoor fish for after obtain product, it is more that it is retained in prepared Chinese ink in squid body.For the Sepiella maindroni product propagated artificially of fishing for, following technology can be taked to get ink.
1) artificial or mechanically peel Sepiella maindroni ink sac;
2) ink sac is preserved: the ice that generally can fall apart is preserved; Large for quantity, the long period person that gets prepared Chinese ink need be waited for, can stored frozen;
3) directly get prepared Chinese ink from ink sac: extruding or squeezing, extrude prepared Chinese ink, essence filter again after gauze coarse filtration;
4) prepared Chinese ink enriched material preparation: prepared Chinese ink filtrate naturally dried in the shade or heat drying, both obtained melanochrome crude product;
5) for increasing operation rate, will extrude the ink sac after prepared Chinese ink, also used water washs 3 times, discards tissue, collects filtrate, for subsequent use; Sepia in water is intercepted with whirlwind-type settling vessel, concentrated, dry, obtain pitch-dark pigment raw product.
(2) melanochrome raw product is prepared from freezing siphonopods marine organisms
1) thaw;
2) artificial or mechanically peel Sepiella maindroni ink sac;
3) directly prepared Chinese ink is got from ink sac: the warm ink sac that makes softens, and extruding or squeezing, extrude prepared Chinese ink, essence filter again after gauze coarse filtration.To the ink sac after prepared Chinese ink be extruded, and wash 3 times with warm water, discard tissue, collect filtrate, for subsequent use; Sepia in water is intercepted with whirlwind-type settling vessel, concentrated, dry, obtain melanochrome raw product;
4) prepared Chinese ink enriched material preparation: prepared Chinese ink filtrate naturally dried in the shade or heat drying, both obtained melanochrome raw product;
5) in artificial or mechanically peel Sepiella maindroni ink sac process containing other viscera tissues of Sepiella maindroni, by " 3, siphonopods processing fent thing extract " preparation after collecting.
(3) from the ink-jet water of dredger, melanochrome raw product is prepared: cryopreservation, for subsequent use; Sepia in water is intercepted with whirlwind-type settling vessel, concentrated, dry, to obtain final product.
2, from industrialization circulating water cultivation backwater, prepare melanochrome raw product: be made up of whirlwind-type settling vessel, sieve-bend screen, multidirectional jet submersible formula Airfloat pump, intercept the suspended particulate in the solid particulate matter in sepia in cultivation backwater, separation cultivation backwater, air supporting intercepting cultivation backwater respectively.The prepared Chinese ink enriched material collected respectively, solid particulate matter, suspended particulate are merged dry, to obtain final product.
3, from siphonopods processing fent thing, prepare melanochrome raw product: thawed by siphonopods processing fent thing, machinery blends, and adds 50% ethanolic soln, stir and melanochrome is dissolved, filter, collect filtrate; Residue uses 50% extraction using alcohol again, collects filtrate.Merging filtrate, reclaims ethanol, both obtains melanochrome raw product.
(2) the melanic indudstrialized refining method of cephalopod
1, as required, be normal temperature to 50 DEG C with the Sumizyme MP of different ratios in temperature, pH value 9 ~ 12 and concentration of substrate are enzymolysis 5 ~ 8h under the condition of 1 ~ 5%, filter, discard residue, filtrate is dry, both obtain the melanochrome of the high purity (98.5%) of different molecular weight.
2, as required, be normal temperature to 50 DEG C with the liming (also available hydrogen sodium oxide) of different ratios in temperature, alkaline hydrolysis 5 ~ 8h under pH value 9 ~ 12 condition, filters, discards residue, and filtrate is dry, both the melanochrome of high purity (98.9%) of different molecular weight.
3, as required, be normal temperature to 50 DEG C with the acetic acid of different ratios (also can with hydrochloric acid) in temperature, alkaline hydrolysis 5 ~ 7h under pH value 1 ~ 4 condition, filters, discards residue, and filtrate is dry, both the melanochrome of high purity (99.6%) of different molecular weight.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (9)
1. the melanic industrialized preparing process of cephalopod, is characterized in that: the preparation and the melanic indudstrialized refining of cephalopod that comprise cephalopod melanochrome raw product.
2. method according to claim 1, is characterized in that: the preparation of cephalopod melanochrome raw product comprises to extract melanochrome raw product, extracts melanochrome raw product from freezing Sepiella maindroni, extracts melanochrome raw product from the ink-jet water of dredger, extract melanochrome raw product and extract melanochrome raw product from industrialization cyclic culture backwater from cephalopod processing fent from Sepiella maindroni live body.
3. method according to claim 2, is characterized in that: from Sepiella maindroni live body or from freezing Sepiella maindroni, extract melanochrome raw product comprise the following steps:
(1) artificial or mechanically peel Sepiella maindroni ink sac;
(2) adopt the mode of extruding or squeezing, extruded by the prepared Chinese ink in ink sac, after gauze coarse filtration, essence filter again, obtains smart filtrate;
(3) will the ink sac of prepared Chinese ink be extruded, and wash 3 times with warm water, discard tissue, collect filtrate, and intercept prepared Chinese ink in water, merge with the smart filtrate of step (2);
(3) concentrated, dry, obtain cephalopod melanochrome raw product.
4. method according to claim 2, is characterized in that: from the ink-jet water of dredger, extract melanochrome raw product is intercept prepared Chinese ink in water, through concentrated, dry, obtains cephalopod melanochrome raw product.
5. method according to claim 2, is characterized in that: from industrialization cyclic culture backwater, extract melanochrome raw product comprise the following steps:
(1) sepia in cultivation backwater is intercepted; Be separated the solid particulate matter in cultivation backwater; Intercept the suspended particulate in cultivation backwater;
(2) prepared Chinese ink enriched material, solid particulate matter and the suspended particulate collected respectively are merged dry, obtain cephalopod melanochrome raw product.
6. method according to claim 2, is characterized in that: from cephalopod processing fent, extract melanochrome raw product comprise the following steps:
(1) thawed by cephalopod processing fent, machinery blends, and adds 50wt.% ethanolic soln, stirs and melanochrome is dissolved, and filters, and collects filtrate;
(2) residue extracts with 50wt.% ethanolic soln again, collects filtrate;
(3) filtrate of combining step (1) (2), reclaims ethanol, obtains cephalopod melanochrome raw product.
7. method according to claim 1, it is characterized in that: the melanic indudstrialized refining of cephalopod is for adopting Sumizyme MP at normal temperature to 50 DEG C, pH=9 ~ 12 and concentration of substrate are enzymolysis 5 ~ 8h under the condition of 1 ~ 5wt.%, filter, discard residue, filtrate is dry, obtain highly purified cephalopod melanochrome.
8. method according to claim 1, it is characterized in that: the melanic indudstrialized refining of cephalopod is for adopting liming or sodium hydroxide solution at normal temperature to 50 DEG C, alkaline hydrolysis 5 ~ 8h under the condition of pH=9 ~ 12, filter, discard residue, filtrate is dry, obtain highly purified cephalopod melanochrome.
9. method according to claim 1, is characterized in that: the melanic indudstrialized refining of cephalopod be adopt acetic acid or hydrochloric acid at normal temperature to 50 DEG C, alkaline hydrolysis 5 ~ 7h under the condition of pH=1 ~ 4, filter, discard residue, filtrate is dry, obtain highly purified cephalopod melanochrome.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875772A (en) * | 2016-06-15 | 2016-08-24 | 大连民族大学 | Cuttlefish juice sugarless soda biscuit and making method thereof |
| CN106009762A (en) * | 2016-05-20 | 2016-10-12 | 佛山市聚成生化技术研发有限公司 | Novel ink melanin extraction method |
| CN106189355A (en) * | 2016-08-11 | 2016-12-07 | 熊廷珍 | Extract the melanic method of squid heath-function |
| CN107195883A (en) * | 2017-05-31 | 2017-09-22 | 青岛大学 | A kind of preparation method of nitrogen-doped carbon nanosphere/molybdenum disulfide sodium ion composite nano materials |
| CN108795093A (en) * | 2017-11-02 | 2018-11-13 | 安发（福建）生物科技有限公司 | A kind of preparation of the pitch-dark pigment of marine animal solubility and analysis method |
| CN108795092A (en) * | 2017-11-02 | 2018-11-13 | 安发（福建）生物科技有限公司 | A kind of preparation method of the pitch-dark pigment of marine animal |
| CN108795095A (en) * | 2017-11-02 | 2018-11-13 | 安发（福建）生物科技有限公司 | A kind of drying means of the pitch-dark pigment of marine animal |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106009762A (en) * | 2016-05-20 | 2016-10-12 | 佛山市聚成生化技术研发有限公司 | Novel ink melanin extraction method |
| CN105875772A (en) * | 2016-06-15 | 2016-08-24 | 大连民族大学 | Cuttlefish juice sugarless soda biscuit and making method thereof |
| CN106189355A (en) * | 2016-08-11 | 2016-12-07 | 熊廷珍 | Extract the melanic method of squid heath-function |
| CN106189355B (en) * | 2016-08-11 | 2017-10-31 | 安徽阳光药业有限公司 | The method for extracting squid heath-function melanin |
| CN107195883A (en) * | 2017-05-31 | 2017-09-22 | 青岛大学 | A kind of preparation method of nitrogen-doped carbon nanosphere/molybdenum disulfide sodium ion composite nano materials |
| CN108795093A (en) * | 2017-11-02 | 2018-11-13 | 安发（福建）生物科技有限公司 | A kind of preparation of the pitch-dark pigment of marine animal solubility and analysis method |
| CN108795092A (en) * | 2017-11-02 | 2018-11-13 | 安发（福建）生物科技有限公司 | A kind of preparation method of the pitch-dark pigment of marine animal |
| CN108795095A (en) * | 2017-11-02 | 2018-11-13 | 安发（福建）生物科技有限公司 | A kind of drying means of the pitch-dark pigment of marine animal |
| CN108795095B (en) * | 2017-11-02 | 2020-03-24 | 安发（福建）生物科技有限公司 | Drying method of marine animal melanin |
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