CN106187466A - Culture medium of edible fungus of high protein selenium-rich and its preparation method and application - Google Patents
Culture medium of edible fungus of high protein selenium-rich and its preparation method and application Download PDFInfo
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- CN106187466A CN106187466A CN201610538303.8A CN201610538303A CN106187466A CN 106187466 A CN106187466 A CN 106187466A CN 201610538303 A CN201610538303 A CN 201610538303A CN 106187466 A CN106187466 A CN 106187466A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to culture medium of edible fungus, specially culture medium of edible fungus of high protein selenium-rich and its preparation method and application, collect edible fungi residue, after shining dry grinding, cross 20 eye mesh screens；With chicken manure, Testa Tritici, EM bacterium stock solution and fish flour, water-stop fermentation, add sodium selenite, access fly blow, after 45 days, the fly larvae entirety of culture matrix and inside thereof is dried, ground, makes high protein selenium-rich culture matrix；It is mixed in proportion as required with adjuvant, makes high protein selenium-rich culture medium of edible fungus.The culture medium of edible fungus of the high protein selenium-rich that the present invention provides, the Utilizing question of edible fungi residue is solved by bioconversion, virose inorganic selenium are converted into and more utilize the organic selenium absorbed, it is made for the culture medium of edible fungus rich in albumen and organic selenium, produce the health edible fungi of organic selenium-rich, increase the economic worth of edible fungi, existing significant ecological benefits, obvious economic benefit can be produced again.
Description
Technical field
The invention belongs to culture medium of edible fungus, specially culture medium of edible fungus of high protein selenium-rich and preparation method thereof
And application.
Background technology
Edible fungi because it is nutritious, delicious flavour and extremely common people's favor.China is that edible fungi recognizes, utilizes, cultivates
Country the earliest, after reform and opening-up, edible fungi of china industry development achieves prominent achievement.Edible fungi of china total output in 1988
Leaping to the first in the world, within 2011, national Edible Fungi total amount reaches 2571.7 ten thousand tons, accounts for more than the 70% of whole world total output, produces
Value, more than 140,000,000,000 yuan, is exported goods and earned foreign currency 24.07 hundred million dollars.Along with the development of mushroom industry, China the most at least produces 4,000,000
The dreg of ton.Process the traditional method of these dregs be abandon or burn, planting material as other edible fungi, animal feed,
Crops or vegetable fertilizer etc., these methods may pollute the environment or cause the waste of agricultural organic resource, it is therefore desirable to grinds
Study carefully edible fungi residue ecology processing method.The cultivation of edible fungi needs to improve further added value simultaneously, increases health care
Function.
Summary of the invention
The present invention be in order to solve the deficiency of existing edible fungi residue treatment, edible fungi added value is difficult to the problems such as raising,
Provide a kind of edible fungi residue utilizing Musca domestica larva to convert interpolation inorganic selenium and produce selenium-rich high protein edible bacterium culture medium matter
Method.
The above-mentioned purpose of the present invention is realized by following technical scheme:
The culture medium of edible fungus of high protein selenium-rich, preparation method comprises the following steps:
Step (1): collect the dreg after edible fungi results, after shining dry grinding, crosses 20 eye mesh screens；
Step (2): the dreg after sieving mixes with the part by weight of 5-7:2-4:1 with chicken manure, Testa Tritici, is by weight
The ratio of 1% adds EM bacterium stock solution and the fish flour of 1%, add water to water content be 40-50%, sealing and fermenting, make fly larvae training
Support substrate；
Step (3): add sodium selenite solution in the ratio of 5-15mg/kg in fly larvae basal medium matter, make richness
Selenium fly larvae culture medium.
Step (4): selenium-rich fly larvae culture matrix is placed in the cement conversion pool of 120cm × 60cm × 20cm；
Step (5): access Eggs of Musca Domestica Vicina in the ratio of 5000-10000 grain/kg in selenium-rich fly larvae culture medium, or press 4000-
The ratio of 9000/kg accesses Sarcophga fuscicauda ovum or lucilia sericata ovum, is placed in 25-30 DEG C, under conditions of humidity is 60-75%
Cultivate, after 4-5 days, the fly larvae entirety of culture matrix and inside thereof is dried, ground, makes high protein selenium-rich culture medium
Matter.
Step (6): mix by different proportion with wood sawdust, cotton seed hulls, Gypsum Fibrosum powder, sucrose etc. with high protein selenium-rich culture matrix
Close, as high protein selenium-rich culture medium of edible fungus.
Wherein, step (1) described edible fungi residue includes Pleurotus ostreatus, Flammulina velutiper (Fr.) Sing, Grifola frondosa, Pleurotus eryngii, Pleurotus nebrodensis, Auricularia polytricha (Mout) Sacc.
Etc. the culture medium residue being suitable to pocket type cultivation edible fungi.
The culture medium of edible fungus of this high protein selenium-rich is for the edible fungi cultivated, including Pleurotus ostreatus, Flammulina velutiper (Fr.) Sing, Grifola frondosa, Fructus Pruni
Abalone mushroom, Pleurotus nebrodensis, Auricularia polytricha (Mout) Sacc., Hericium erinaceus (Bull. Ex Fr.) Pers., Lentinus Edodes, Cordyceps militaris (L.) Link. etc. are suitable to pocket type cultivation edible fungi, also include Caulis Bambusae In Taeniam, Coprinus comatus etc.
The edible fungi of earthing formula cultivation.
Selenium is one of trace element required in human life activity, is the antioxidant in human body, can improve human body and exempt from
Epidemic disease power, has multiple biological function.Selenium is in addition to can preventing and treating Keshan disease, Kaschin-Beck disease, moreover it is possible to antiviral, antiallergic, defying age, disappear
Inflammation, aspergillus flavus resisting toxin B1 and the carcinogenesis such as benzanthracene, nitrosamine, alleviate the toxic and side effects of radiotherapy, chemotherapy.Selenium supplement mainly leads to
Cross direct oral or intravenous selenium compound, and Excess free enthalpy selenium can cause selenosis.The high protein selenium-rich that the present invention provides
Culture medium of edible fungus, by bioconversion inorganic selenium produce organic selenium method, improve selenium supplement safety.
Fly larvae is a kind of preferably bioconversion material, and its growth rapidly, is suitable for all feeds and environment, and conduct
Feed protein additive is of high nutritive value, and containing thick protein 53.26-62%, crude fat 13.09%, various amino acid/11s 7 kinds, is
A kind of high protein is biological, and high protein fertilizer can act on the excellent of high added value industrial crops, flowers, fruit tree, Chinese crude drug etc.
Matter fertilizer.
Converted the edible fungi residues adding inorganic selenium by fly larvae, high protein selenium-rich material substrate can be produced, can after adding adjuvant
For high protein selenium-rich culture medium of edible fungus, both can solve the Ecological Disposal problem of edible fungi residues, mushroom industry can be extended again
Chain, improves the economic worth of edible fungi.
The culture medium of edible fungus of the high protein selenium-rich that the present invention provides, solves the profit of edible fungi residue by bioconversion
By problem, virose inorganic selenium are converted into and more utilize the organic selenium absorbed, be made for rich in albumen and organic selenium
Culture medium of edible fungus, produces the health edible fungi of organic selenium-rich, increases the economic worth of edible fungi, existing significant Ecological Effect
Benefit, can produce again obvious economic benefit.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1
The culture medium of edible fungus of high protein selenium-rich, is prepared gained by following methods:
(1) collect Pleurotus ostreatus edible fungi residue, after shining dry grinding, cross 20 eye mesh screens；
(2) dreg after sieving mixes with the ratio of weight ratio 5:4:1 with chicken manure, Testa Tritici, is 1% by gross weight ratio
Ratio adds EM bacterium stock solution and the fish flour of 1%, add water to water content be 40-50%, sealing and fermenting, make fly larvae culture medium
Matter；
(3) in fly larvae culture matrix, add sodium selenite in the ratio of 10mg/kg, make selenium-rich fly larvae culture medium；Connect
Enter fly blow, access Eggs of Musca Domestica Vicina in the ratio of 5000-10000 grain/kg.
(4) selenium-rich fly larvae culture matrix is placed in conversion pool；
(5) in selenium-rich fly larvae culture medium, access fly blow, be placed in 25-30 DEG C, humidity be to train under conditions of 60-75%
Support, after 4-5 days, the fly larvae entirety of culture matrix and inside thereof is dried, ground, makes high protein selenium-rich culture matrix；
(6) mix according to weight ratio 60:35:1:1 with wood sawdust, cotton seed hulls, Gypsum Fibrosum powder, sucrose with high protein selenium-rich culture medium
Close, make high protein selenium-rich culture medium of edible fungus.
For carrying out the pocket type cultivation of Lentinus Edodes.Meanwhile, with cotton seed hulls: saw dust: sucrose: the Lentinus Edodes that Gypsum Fibrosum powder ratio is made
Culture medium is comparison.
Result shows, housefly maggot is after selenium-rich culturing base is multiplied, and its internal Se content averagely reaches 1.93mg/kg；And
Reaching 0.46mg/kg with its average content being made the Selenium in Mushroom that high protein selenium-rich culture medium is cultivated, organic selenium content is higher,
And within safety range.Meanwhile, high protein selenium-rich culture medium can promote the growth of Lentinus Edodes, the freshest mushroom weight, bacteria cover diameter etc.
Index is all remarkably higher than control medium (table 1).
Table 1 high protein selenium-rich culture medium and the control medium impact on mushroom growth
| Culture medium classification | Mushroom fresh weight (g) | Bacteria cover diameter (cm) | Stem length (cm) | Stem diameter |
| High protein selenium-rich culture medium | 31.3±2.1a | 7.4±0.4a | 2.6±0.1a | 1.7±0.1a |
| Control medium | 24.3±1.4b | 6.6±0.3b | 2.4±0.1a | 1.5±0.1a |
Same column of figure, different letters represent significant difference (p < 0.05).
Embodiment 2
The culture medium of edible fungus of high protein selenium-rich, is prepared gained by following methods:
(1) collect Flammulina velutiper (Fr.) Sing, Grifola frondosa edible fungi residue, after shining dry grinding, cross 20 eye mesh screens；
(2) dreg after sieving mixes with the ratio of weight ratio 7:3:1 with chicken manure, Testa Tritici, is 1% by gross weight ratio
Ratio adds EM bacterium stock solution and the fish flour of 1%, add water to water content be 40-50%, sealing and fermenting, make fly larvae culture medium
Matter；
(3) in fly larvae culture matrix, add sodium selenite in the ratio of 15mg/kg, make selenium-rich fly larvae culture medium；Connect
Enter fly blow, access Sarcophga fuscicauda ovum in the ratio of 4000-8000 grain/kg.
(4) selenium-rich fly larvae culture matrix is placed in conversion pool；
(5) in selenium-rich fly larvae culture medium, access fly blow, be placed in 25-30 DEG C, humidity be to train under conditions of 60-75%
Support, after 4-5 days, the fly larvae entirety of culture matrix and inside thereof is dried, ground, makes high protein selenium-rich culture matrix；
(6) mix according to weight ratio 62:35:1:1 with wood sawdust, cotton seed hulls, Gypsum Fibrosum powder, sucrose with high protein selenium-rich culture medium
Close, make high protein selenium-rich culture medium of edible fungus.
For carrying out Pleurotus ostreatus pocket type cultivation.
Embodiment 3
The culture medium of edible fungus of high protein selenium-rich, is prepared gained by following methods:
(1) collect Pleurotus eryngii, Auricularia polytricha (Mout) Sacc. edible fungi residue, after shining dry grinding, cross 20 eye mesh screens；
(2) dreg after sieving mixes with the ratio of weight ratio 6:2:1 with chicken manure, Testa Tritici, is 1% by gross weight ratio
Ratio adds EM bacterium stock solution and the fish flour of 1%, add water to water content be 40-50%, sealing and fermenting, make fly larvae culture medium
Matter；
(3) in fly larvae culture matrix, add sodium selenite in the ratio of 5mg/kg, make selenium-rich fly larvae culture medium；Access
Fly blow, accesses lucilia sericata ovum in the ratio of 4000-8000 grain/kg.
(4) selenium-rich fly larvae culture matrix is placed in conversion pool；
(5) in selenium-rich fly larvae culture medium, access fly blow, be placed in 25-30 DEG C, humidity be to train under conditions of 60-75%
Support, after 4-5 days, the fly larvae entirety of culture matrix and inside thereof is dried, ground, makes high protein selenium-rich culture matrix；
(6) mix according to weight ratio 60:35:1:1 with wood sawdust, cotton seed hulls, Gypsum Fibrosum powder, sucrose with high protein selenium-rich culture medium
Close, make high protein selenium-rich culture medium of edible fungus.
Cultivate for Caulis Bambusae In Taeniam earthing formula.
Claims (5)
1. the culture medium of edible fungus of high protein selenium-rich, it is characterised in that prepared gained by following methods, preparation method, including with
Lower step:
(1) collect edible fungi residue, after shining dry grinding, cross 20 eye mesh screens；
(2) dreg after sieving mixes with the ratio of weight ratio 5-7:2-4:1 with chicken manure, Testa Tritici, is 1% by gross weight ratio
Ratio adds EM bacterium stock solution and the fish flour of 1%, add water to water content be 40-50%, sealing and fermenting, make fly larvae culture medium
Matter；
(3) in fly larvae culture matrix, add sodium selenite in the ratio of 5-15mg/kg, make selenium-rich fly larvae culture medium；
(4) selenium-rich fly larvae culture matrix is placed in conversion pool；
(5) in selenium-rich fly larvae culture medium, access fly blow, be placed in 25-30 DEG C, humidity be to cultivate under conditions of 60-75%,
After 4-5 days, the fly larvae entirety of culture matrix and inside thereof is dried, ground, makes high protein selenium-rich culture matrix；
(6) it is mixed in proportion as required with adjuvant with high protein selenium-rich culture medium, makes high protein selenium-rich culture medium of edible fungus.
The culture medium of edible fungus of high protein selenium-rich the most according to claim 1, it is characterised in that step (1) is described edible
Bacterium dreg is to be suitable to the culture medium residue of pocket type cultivation edible fungi.
The culture medium of edible fungus of high protein selenium-rich the most according to claim 1, it is characterised in that connecing described in step (5)
Enter fly blow, access Eggs of Musca Domestica Vicina in the ratio of 5000-10000 grain/kg, or it is the numbest to access palm fibre tail in the ratio of 4000-8000 grain/kg
Fly blow or lucilia sericata ovum.
The culture medium of edible fungus of high protein selenium-rich the most according to claim 1, it is characterised in that described in step (6)
Adjuvant includes wood sawdust, cotton seed hulls, Gypsum Fibrosum powder, sucrose.
5. according to the application of the culture medium of edible fungus of the high protein selenium-rich described in any one of claim 1 to 4, it is characterised in that
For cultivating edible fungi and the edible fungi of earthing formula cultivation of pocket type cultivation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610538303.8A CN106187466A (en) | 2016-07-08 | 2016-07-08 | Culture medium of edible fungus of high protein selenium-rich and its preparation method and application |
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| CN201610538303.8A CN106187466A (en) | 2016-07-08 | 2016-07-08 | Culture medium of edible fungus of high protein selenium-rich and its preparation method and application |
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| CN201610538303.8A Pending CN106187466A (en) | 2016-07-08 | 2016-07-08 | Culture medium of edible fungus of high protein selenium-rich and its preparation method and application |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109247196A (en) * | 2018-11-19 | 2019-01-22 | 遵义周星星菌业有限公司 | A kind of edible mushroom selenium-rich culturing base |
| CN112314420A (en) * | 2020-11-09 | 2021-02-05 | 湖北省农业科学院经济作物研究所 | Chinese cabbage water culture method and nutrient solution thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805201A (en) * | 2011-06-01 | 2012-12-05 | 刘成军 | Method for making magnetic fly maggot feed |
| CN102960184A (en) * | 2012-11-22 | 2013-03-13 | 兴隆县庆旺富硒食用菌农民专业合作社 | Edible fungus strain rich in organic selenium, preparation method therefore, edible fungus rich in organic selenium and culturing method thereof |
| CN103583901A (en) * | 2013-11-07 | 2014-02-19 | 周辛平 | Fly and maggot cultivation method and high protein feed adopting same |
| CN104785509A (en) * | 2015-04-17 | 2015-07-22 | 泰山医学院 | Method for bioconversion of edible mushroom dregs with housefly larvae |
-
2016
- 2016-07-08 CN CN201610538303.8A patent/CN106187466A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805201A (en) * | 2011-06-01 | 2012-12-05 | 刘成军 | Method for making magnetic fly maggot feed |
| CN102960184A (en) * | 2012-11-22 | 2013-03-13 | 兴隆县庆旺富硒食用菌农民专业合作社 | Edible fungus strain rich in organic selenium, preparation method therefore, edible fungus rich in organic selenium and culturing method thereof |
| CN103583901A (en) * | 2013-11-07 | 2014-02-19 | 周辛平 | Fly and maggot cultivation method and high protein feed adopting same |
| CN104785509A (en) * | 2015-04-17 | 2015-07-22 | 泰山医学院 | Method for bioconversion of edible mushroom dregs with housefly larvae |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109247196A (en) * | 2018-11-19 | 2019-01-22 | 遵义周星星菌业有限公司 | A kind of edible mushroom selenium-rich culturing base |
| CN112314420A (en) * | 2020-11-09 | 2021-02-05 | 湖北省农业科学院经济作物研究所 | Chinese cabbage water culture method and nutrient solution thereof |
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