CN106176871A - A kind of Fructus Hippophae compound recipe preventing and treating influenza and preparation thereof and application - Google Patents
A kind of Fructus Hippophae compound recipe preventing and treating influenza and preparation thereof and application Download PDFInfo
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Abstract
The present invention relates to a kind of Fructus Hippophae compound recipe preventing and treating influenza and preparation method thereof, described Fructus Hippophae compound recipe includes following component: Fructus Hippophae, Herba Taraxaci, Radix Isatidis.The present invention passes through Fructus Hippophae compatibility Herba Taraxaci and Radix Isatidis, the most directly, the effective activity suppressing influenza virus, and by regulating the immunologic function of human body, improves the immunity of human body, strengthens the human body resistance to pathogenic bacteria, thus reached the effect for the treatment of both the principal and secondary aspects of a disease.Therefore, popularity of the present invention flu has more preferable curative effect.Preparation method of the present invention is simple, it is easy to accomplish industrialized production.It addition, the Fructus Hippophae compound preparation that the present invention provides is pure natural medicinal plants formula, the side effect that human body is not caused because of any chemical composition.
Description
Technical field
The present invention relates to a kind of Chinese medicine compound, be specifically related to a kind of Fructus Hippophae compound recipe preventing and treating influenza and system thereof
Preparation Method.
Background technology
Fructus Hippophae is shrub or the dungarunga of Elaeangnaceae acid thorn genus, another name vinegar willow, black thorn, acid thorn.Early
In ancient Greek, the battle steed of the old,weak,sick and disabled becomes plump and sturdy after having eaten Fructus Hippophae, and hair color is shinny.Three states
In the epoch, Shu State military counsellor Zhuge Liang is in once the going on an expedition of Yunnan, owing to The distance is far and the mountain is high, st unfamiliar with the people and the place,
Disease popularity, forces fall ill countless, have had a strong impact on the fighting capacity of army.It was found that a kind of red later
The fruit of yellow, is Fructus Hippophae, has eaten the later forces that become strong, and popular disease also disappears
?.Fructus Hippophae is just described as far back as 8th century of Christian era Tibetan medicine's masterpiece " moon king's drug eruption " and the Four-Volume Medical Code
There is effect of heat-clearing and toxic substances removing." traditional Chinese medical science voluminous dictionary " record Fructus Hippophae have have blood circulation promoting and blood stasis dispelling, the chest stuffiness relieving that reduces phlegm,
Spleen reinforcing stomach invigorating, promoting the production of body fluid to quench thirst, effect of clearing away heat to stop diarrhea.
Sea buckthorn fruit, rich in medicinal component, has many effects.Particularly sea buckthorn juice has increasing
The function of strong body immunity.Its treatment panimmunity system clinical available, immunologic hypofunction and
Disease (Deng little Juan etc., the China's Pharmaceutical that human metabolism's function is not enough；2009 volume 18: 63-64).
Therefore, Fructus Hippophae resists the invasion to human body of pathogenic bacteria or virus by improving immunity of human body itself.
Research finds, Fructus Hippophae is viral to influenza A and B, and to adenovirus, paramyxovirus, herpes simplex
Virus (Herpes catarrhalis), cytomegalovirus and respiratory syncytial virus show significantly suppression
Effect.
Patent of invention CN102771600A uses Radix Isatidis and Herba Taraxaci to make drink tea, and its advantage is can
To prevent and treat influenza, it is passive by the popular pathogenic bacteria of suppression to have a disadvantage in that, and can not be actively
The immunologic function of regulation human body self, thus take stopgap measures and can not effect a permanent cure, the effect of prevention can only be played.
If adding this medical material simply of Fructus Hippophae improving body immunity, in prevention with treat grippal
Effect may be good many.Visible, the importance of this taste medicine of Fructus Hippophae.
In patent of invention CN102727838A, Herba Taraxaci and Fructus Hippophae compatibility is used to carry out anti-flu and drive
Cold, for treating the fever symptoms in flu.Its advantage is Herba Taraxaci and Fructus Hippophae compatibility, passes through Fructus Hippophae
Improving human body makes patient bring down a fever the resistance of pathogenic bacteria, helps with cold Herba Taraxaci pathogenic fire reducing simultaneously, reaches
To bringing down a fever and the effect of anti-flu.But, this patent lacks a herb material: Radix Isatidis.Baphicacanthus cusia
Root is one of viral optimal Chinese crude drug of the treatment and prevention generally acknowledged at present, at China's bird flu and Sa Si
The treatment and prevention of virus serve critically important effect.If addition Radix Isatidis, significantly strengthen
The effect of suppression cold virus, reaches treating both the principal and secondary aspects of a disease, and the therapeutic effect of medicine is more preferably.
But, the effect of existing compound recipe is poor, accordingly, it is desirable to provide a kind of more preferable compound recipe of effect.
Summary of the invention
The present invention relates to a kind of Fructus Hippophae compound recipe preventing and treating influenza.
A kind of Fructus Hippophae compound recipe preventing and treating influenza that the present invention provides, it includes following component: Fructus Hippophae, Pu
Herba Taraxaci, Radix Isatidis.
Concrete, described Fructus Hippophae compound recipe includes the composition of following weight portion: Fructus Hippophae 2-80 part, Herba Taraxaci 2-40
Part, Radix Isatidis 4-80 part.
Preferably, described Fructus Hippophae method includes the composition of following weight portion: Fructus Hippophae 20-60 part, Herba Taraxaci
10-30 part, Radix Isatidis 15-45 part.
Further preferably, described Fructus Hippophae method includes the composition of following weight portion: Fructus Hippophae 40 parts, Pu Gong
English 20 parts, Radix Isatidis 30 parts.
Possibly together with following component in described Fructus Hippophae compound recipe: Fructus Mori, Radix Glycyrrhizae and Radix Et Rhizoma Fagopyri Tatarici, at described compound recipe
In, the weight portion of above-mentioned three kinds of compositions is: Fructus Mori 1-20 part, Radix Glycyrrhizae 1-20 part and Radix Et Rhizoma Fagopyri Tatarici 1-20 part.
Preferably, the weight portion of above-mentioned three kinds of compositions is: Fructus Mori 1-10 part, Radix Glycyrrhizae 1-8 part and Radix Et Rhizoma Fagopyri Tatarici
1-8 part.
Further preferably, the weight portion of above-mentioned three kinds of compositions is: Fructus Mori 4 parts, 3 parts of Radix Glycyrrhizae and Radix Et Rhizoma Fagopyri Tatarici 3
Part.
It is a further object of the present invention to provide the preparation method of Fructus Hippophae compound recipe, the method comprises the following steps:
Fructus Hippophae, Fructus Mori, Herba Taraxaci, Radix Glycyrrhizae, Radix Et Rhizoma Fagopyri Tatarici, Radix Isatidis is weighed according to proportioning, standby；
Respectively Radix Glycyrrhizae, Radix Et Rhizoma Fagopyri Tatarici, Radix Isatidis are pulverized, add water after mixing, at a temperature of 65-100 DEG C
Extract 1-10 hour, obtain water extract；
Respectively by Fructus Hippophae, Fructus Mori and Herba Taraxaci lyophilization, grind to form fine powder, add above-mentioned water logging
Body fluid, to obtain final product.
It is a further object of the present invention to provide the preparation containing above-mentioned Fructus Hippophae compound recipe, described preparation is multiple by Fructus Hippophae
Square and pharmaceutically acceptable carrier forms.
Described preparation is granule, and on granule Chinese materia medica, acceptable carrier is the conventional load of this area
Body, such as dextrin, sucrose.
Final object of the present invention is to provide Fructus Hippophae compound recipe at preparation prevention and the medicine for the treatment of influenza
In application, described influenza is grippal abbreviation.
The Fructus Hippophae compound recipe that the present invention provides has the advantage that
1, in the effect for the treatment of influenza
The present invention is found by influenza virus Inhibition test, Fructus Hippophae and Herba Taraxaci, Radix Isatidis compatibility, no
The effect (suppression ratio be up to 98%) of suppression influenza activity is only greatly improved.And, logical
Crossing in mice Antibody of Influenza and experiment finds, Fructus Hippophae can significantly carry with Herba Taraxaci, Radix Isatidis combination
Senior middle school and the titre of Antibody of Influenza, strengthen the immunity of mice body, thus improve mice pair
The resistivity of influenza virus.
Therefore, Fructus Hippophae compound recipe cold granules of the present invention, be treatment influenza virus very reliable,
Effective Chinese medicine composition.
2, the Fructus Hippophae compound recipe that the present invention provides all uses pure natural medical material, does not has any side effect to human body,
This for infant health not yet reach maturity and Old Men weakness for, this medical instrument has important
Practical value.
3, Fang Xie, in the Fructus Hippophae compound recipe that the present invention provides:
Fructus Hippophae (Hippophae rhamnoides Linn.): another name is vinegar willow, yellow acid stings, willow is stung in acid,
Black thorn, acid thorn.Elaeangnaceae Hippophae.Machaka, xylophyta.Sour in the mouth, puckery, warm in nature.
Relieving cough and resolving phlegm, invigorating the stomach and promoting digestion, blood circulation promoting and blood stasis dispelling.
Fructus Mori (Fructus Mori): have another name called into Mulberry reality, Shen, crow mulberry, civil and military mulberry real, black, Mulberry Fructus Jujubae,
Fructus Mori, Sang Li, sorosis.Moraceae Morus.Deciduous tree, xylophyta.Sweet in the mouth is sour, cold in nature.
Nourishing YIN and supplementing blood, promote the production of body fluid moisturize, the having a dizzy spell of deficiency of the liver and kindey, blood deficiency damage of essence, soreness of waist tinnitus, beard and hair
Early white, insomnia and dreamful sleep, Tianjin wound are thirsty, quench one's thirst, dryness of the intestine constipation.Mulberry juice has for influenza
Having certain treatment and prevention effect, Fructus Mori leaf cures mainly anemopyretic cold.
Herba Taraxaci (Taraxacum mongolicum Hand.-Mazz.): call into Hua Hualang, Pu Gongcao,
Edible Herba Taraxaci, Herba Taraxaci careless, Western of wetting the bed.Compositae Dandelion.Herbaceos perennial.Taste
Bitter, cold in nature.Heat-clearing and toxic substances removing.Can be used for pyretic toxicity disease, be especially apt to removing heat from the liver, treat liver-heat conjunctival congestion and swelling pain,
And multi-infection, purulent disease.To staphylococcus aureus, Bacillus typhi, bacillus pyocyaneus,
Sporidiole bacteria, the most blue achorion, trichophyton etc. have inhibitory action；Antifungal and antitumor.
Radix Glycyrrhizae (Glycyrrhiza uralensis Fisch): have another name called, Herba Hedyotis cantonensis, sweet root old into state.Pulse family
Glycyrrhiza.Herbaceos perennial.Sweet in the mouth, property put down, is in harmonious proportion hundred medicines.There is invigorating the spleen and replenishing QI, treatment
Flu, heat-clearing and toxic substances removing, go the function of phlegm and relieving cough, relieving spasm to stop pain.For weakness of the spleen and stomach, lassitude,
Shortness of breath and palpitation, cough with copious phlegm, gastral cavity abdomen, extremity contraction urgency, pain, carbuncle sore tumefacting virus, and can cushion
Toxicity, strong.
Radix Et Rhizoma Fagopyri Tatarici (Fagopyrum tataricum): another name spinach wheat, Wu Mai, Hua Qiao.Annual herb
Plant.Buckwheat plant.Bitter in the mouth, property are flat cold.There are physical strength profiting, continuous knowledge spiritual, sharp, fall
Gas, wide intestinal, the effect of stomach invigorating.Antiinflammatory, cough-relieving, eliminate the phlegm, relieving asthma.Radix Et Rhizoma Fagopyri Tatarici contains morin,
Staphylococcus aureus, dysentery bacterium, herpesvirus and Bacillus typhi there is stronger antibacterial action.
Radix Isatidis (Isatis tinctoria): have another name called as Isatis indigotica Fort., mountain indigo plant, great Lan root, rhizoma et radix baphicacanthis, Lan Long
Root, Lumbricus root, big indigo.Cruciferae Isatis indigotica Fort. belongs to.Biennial herb plant.Bitter in the mouth, cold in nature.Clearly
Thermal detoxification, removing heat from blood, sore-throat relieving.Various bacteria there is inhibitory action.
Fructus Hippophae, warm in nature, sweet, the immunologic function of human body can be significantly improved, and Fructus Hippophae itself has very
The activity of strong suppression influenza virus；Compatibility Herba Taraxaci, cold in nature, bitter, there are heat-clearing and toxic substances removing, Sweeling-eliminating medicine powder
Knot, effect of dampness removing jaundice eliminating；Compatibility Radix Isatidis, cool in nature, cold, bitter, there are heat-clearing and toxic substances removing, removing heat from blood
Effect, can suppress multiple pathogenic bacteria and influenza virus.Therefore, Fructus Hippophae and Herba Taraxaci, Radix Isatidis compatibility,
In treatment common cold and influenza, reach this double effect controlled of table, there is splendid curative effect.
It addition, for the effect strengthening treatment flu further, at Fructus Hippophae, Herba Taraxaci and Radix Isatidis be
On the basis of key agents, it is aided with Radix Et Rhizoma Fagopyri Tatarici, Radix Glycyrrhizae and Fructus Mori, makes the cold, warm, bitter, sweet of the property of medicine
Compatibility more balances, and drug effect is the most definite, reliable.
4, compared with existing document such as CN102771600A and CN102727838A, the present invention provides
Compound recipe effectively overcomes the defect of the two patent, and the curative effect making medicine is the most notable, has important
The practicality of meaning.
Accompanying drawing explanation
Fig. 1 is the direct Inhibition test of Fructus Hippophae compound recipe infected by influenza.Wherein, A represents blank group；
B represents Radix Isatidis+Herba Taraxaci group；C represents Herba Taraxaci+Fructus Hippophae group；D represents patent group of the present invention, husky
Spine+Herba Taraxaci+Radix Isatidis group.
Fig. 2 is the impact on mouse influenza neutralizing antibody of the Fructus Hippophae compound recipe.Wherein, A represents blank group；
B represents Radix Isatidis+Herba Taraxaci group；C represents Herba Taraxaci+Fructus Hippophae group；D represents patent group of the present invention, husky
Spine+Herba Taraxaci+Radix Isatidis group.
Fig. 3 is the direct Inhibition test of Fructus Hippophae compound recipe infected by influenza.Wherein, A represents blank group；
B represents that medicine (Fructus Mori+Radix Glycyrrhizae+Radix Et Rhizoma Fagopyri Tatarici) group assisted a ruler in governing a country by Chinese medicine；C represents Herba Taraxaci+Radix Isatidis+Chinese medicine
Assist a ruler in governing a country medicine group；D represents that medicine group assisted a ruler in governing a country by Herba Taraxaci+Fructus Hippophae+Chinese medicine；E represents Fructus Hippophae+Herba Taraxaci+plate
Medicine group assisted a ruler in governing a country by blue root+Chinese medicine.
Fig. 4 is the impact on mouse influenza neutralizing antibody of the Fructus Hippophae compound recipe.Wherein, A represents blank group；
B represents that medicine (Fructus Mori+Radix Glycyrrhizae+Radix Et Rhizoma Fagopyri Tatarici) group assisted a ruler in governing a country by Chinese medicine；C represents Herba Taraxaci+Radix Isatidis+Chinese medicine
Assist a ruler in governing a country medicine group；D represents that medicine group assisted a ruler in governing a country by Herba Taraxaci+Fructus Hippophae+Chinese medicine；E represents Fructus Hippophae+Herba Taraxaci+plate
Medicine group assisted a ruler in governing a country by blue root+Chinese medicine.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.
The preparation method of embodiment 1 Fructus Hippophae compound recipe cold granules
1, composition: 3200g Fructus Hippophae, 320g Fructus Mori, 1600g Herba Taraxaci, 240g Radix Glycyrrhizae, 240g is bitter
Semen Fagopyri Esculenti, 2400g Radix Isatidis.
2, preparation method
1) respectively Fructus Hippophae, Fructus Mori, Herba Taraxaci, Radix Glycyrrhizae, Radix Et Rhizoma Fagopyri Tatarici, Radix Isatidis Six-element medicine are cleaned,
Remove impurity, check qualified standby.
2) take Fructus Hippophae, Fructus Mori and Herba Taraxaci, lyophilization, grind to form fine powder.
3) respectively Radix Glycyrrhizae, Radix Et Rhizoma Fagopyri Tatarici, Radix Isatidis being added decocting 2 times, temperature is 65-100 DEG C.For the first time
Adding the water yield is 6 times of dose volume；It is 4 times of dose volume that second time adds the water yield.Time is each
Being 2 hours, merge decocting liquid, 5000 × g is centrifuged 20 minutes, collects supernatant, i.e. water extract.
The fine powder that Fructus Hippophae, Fructus Mori and the Herba Taraxaci of above-mentioned preparation are ground to form, with Radix Glycyrrhizae, Radix Et Rhizoma Fagopyri Tatarici and
Radix Isatidis water extract mix homogeneously, adds 2000g adjuvant (dextrin is 6:2 with the proportioning of sucrose),
Stir, be spray dried to granule, or lyophilization becomes granule.Subpackage, is packaged into 1000 little
Bag is standby.
Experimental example 1 Fructus Hippophae compound recipe infected by influenza Inhibition test
1, experiment packet:
This experiment is divided into 4 groups, is A, B, C and D respectively.
A group: blank group, is not added with any medicine.
B group: Radix Isatidis+Herba Taraxaci, proportioning and preparation method reference example 1.
C group: Herba Taraxaci+Fructus Hippophae, proportioning and preparation method reference example 1.
D group: patent group of the present invention, Fructus Hippophae+Herba Taraxaci+Radix Isatidis, proportioning and preparation method reference implementation
Example 1.
2, experiment material.
2.1 Chinese crude drugs: Fructus Hippophae, Herba Taraxaci and Radix Isatidis are purchased in the big pharmacy of Beijing Tongrentang.
2.2 influenza virus: A/California F07/09 (H1N1), this strain virus is stream in 2009
Feel pandemic international representative strains.Preserved by Disease Control and Prevention Center of country virus.
2.3 cell strains: MDCK system Madin-Darby canine kidney(cell line) (MDCK), by the passed on preservation of virus of Disease Control and Prevention Center of country.
3, method.
According to document (Shu Liping, modern combination of Chinese and Western medicine magazine.Volume 18 35 phases: 4339-4340 in 2009)
Method is tested.
3.1 influenza virus pass on the titration with hemagglutinative titer: influenza virus is inoculated in instar chicken embryo on the 9th
Allantoic cavity, cultivates 48h, collects allantoic fluid the most afterwards for 4 DEG C for 37 DEG C by 0.2mL/；Through hemagglutination test
Titration viral hemoagglutination titer is 1/1500, and 1500 × g is centrifuged 5 minutes, takes supernatant, tubule subpackage ,-20
DEG C save backup.
3.2 medicines toxic action to mdck cell: with the DMEM training dual anti-containing penicillin and streptomycin
After Fructus Hippophae, Herba Taraxaci and Radix Isatidis (400mg/mL) are made series two-fold dilution by nutrient solution, it is seeded in
On the MDCK cell monolayers of 1 age in days, each dilution factor inoculates 4 holes (0.1mL/ hole), hatches 2 for 37 DEG C
Hour, abandon supernatant, the cell growth status in each hole of Continuous Observation, and it is right to set DMEM culture fluid blank
According to.
The directly effect of 3.3 medicine infected by influenzas: with Hanks liquid by Fructus Hippophae, Herba Taraxaci and Radix Isatidis
(25mg/ml) do doubling dilution, be separately added into equivalent 1500Hu (HAU) influenza virus, vertical
I.e. 37 DEG C water-baths, after acting on 8 hours, measure hemagglutinative titer respectively.
4, experimental result: through calculating, draw following result, be shown in Table 1 and Fig. 1.
Table 1: the direct suppression ratio of infected by influenza
| Directly suppression ratio | |
| A group | 21% |
| B group | 48% |
| C group | 52% |
| D group | 98% |
Table 1 and Fig. 1 result shows:
1. tetra-groups of A, B, C and D are the least to the toxicity of mdck cell, and cellular morphology is preferable, raw
Long vigorous.
2. the direct suppression ratio of D group (of the present invention group, Fructus Hippophae+Herba Taraxaci+Radix Isatidis) infected by influenza
Reach 98%；The suppression ratio of B group (Radix Isatidis+Herba Taraxaci) is 48%；C group (Herba Taraxaci+Fructus Hippophae)
Suppression ratio 52% (see figure 1).And the suppression ratio of A group (blank group) infected by influenza is
21%.D group (of the present invention group), compared with A, B, C tri-groups, has significant differences (P < 0.01).
As can be seen here, Fructus Hippophae, Herba Taraxaci and Radix Isatidis compatibility, highly significant to enhance convection current susceptible
The inhibitory action of poison, can directly suppress the influenza virus infection to cell.
The impact on mouse influenza virus neutralizing antibody of the experimental example 2 Fructus Hippophae compound recipe
1, experiment packet:
Using mice to test, this experiment is divided into 4 groups, is A, B, C and D respectively.
A group: blank group, is not added with any medicine.
B group: Radix Isatidis+Herba Taraxaci, its proportioning and preparation method reference example 1.
C group: Herba Taraxaci+Fructus Hippophae, its proportioning and preparation method reference example 1.
D group: patent group of the present invention, Fructus Hippophae+Herba Taraxaci+Radix Isatidis, its proportioning and preparation method are with reference to real
Execute example 1.
2, experiment material.
2.1 Chinese crude drugs: Fructus Hippophae, Herba Taraxaci and Radix Isatidis are purchased from the big pharmacy of Beijing Tongrentang.
2.2 influenza virus: Flu virus A/FM/1/47 (H1N1).By Disease Control and Prevention Center of country
Virus is preserved.
2.3 cell strains: MDCK system Madin-Darby canine kidney(cell line) (MDCK), by the passed on preservation of virus of Disease Control and Prevention Center of country.
2.4 laboratory animals: cleaning grade Bal b/c mice, body weight 18-20g, male and female half and half, are cured by Beijing
University of section Animal House provides.
3, experimental technique.
According to document (Zheng Qun etc., Capital University of Medical Sciences's journal.Volume 25 2 phases: 180-182 in 2004) and
(Shu Liping, modern combination of Chinese and Western medicine magazine.Volume 18 35 phases: 4339-4340 in 2009) method carries out
Experiment.
Passing on of 3.1 influenza virus: with experimental example 1.
The detection of 3.2 mice neutralizing antibodies.
Take mice 40, with normal saline, virus liquid is diluted to mice median lethal dose(LD 50) (LD50=10-3.25)
15 times, with the influenza virus collunarium infecting mouse of this titre.
Mice is divided into A, B, C, D tetra-groups, often group 10.With after influenza infection mice 2 days,
Fructus Hippophae, Herba Taraxaci and Radix Isatidis test dose use minimum non-toxic 25mg/ml, according to A, B,
Tetra-groups of C, D, respectively oral administration 5 days, the 6th day starts to detect the NAT of mice.Inspection
The cell surveying neutralizing antibody employing is mdck cell；Eyeball of mouse blood-letting is lethal, separates determination of serum
Neutralizing antibody.
4, experimental result: according to testing result, by calculating, draw following result: be shown in Table 2 and Fig. 2.
Table 2: the impact on NAT
| NAT | |
| A group | 1:180 |
| B group | 1:220 |
| C group | 1:430 |
| D group | 1:580 |
Table 2 and Fig. 2 result shows: A group is the blank group of comparison, and the NAT of mice is 1:180；
B group (Radix Isatidis+Herba Taraxaci), the NAT of mice is 1:220；C group (Herba Taraxaci+Fructus Hippophae),
The NAT of mice is 1:430；D group (of the present invention group, Fructus Hippophae+Herba Taraxaci+Radix Isatidis) is little
The neutralization titre of Mus is 1:580.Visible, D group, compared with A, B, C tri-groups, has very significant poor
Different (P < 0.01).
As can be seen here, Fructus Hippophae, Herba Taraxaci and the Radix Isatidis three's compatibility that the present invention uses, significantly carry
The high neutralizing antibody reaction of mice against influenza virus, i.e. by the immunologic function in regulation Mice Body,
Improve the immunity of mice, strengthen the mice resistance to pathogenic bacteria.
Experimental example 3: Fructus Hippophae compound recipe infected by influenza Inhibition test
1, experiment packet: this experiment is divided into 5 groups, is A, B, C, D and E respectively.
A group: blank group, is not added with any medicine.
B group: Chinese medicine assists a ruler in governing a country medicine (Fructus Mori+Radix Glycyrrhizae+Radix Et Rhizoma Fagopyri Tatarici, the proportioning of each composition and preparation method
Reference example 1).
C group: medicine assisted a ruler in governing a country by Radix Isatidis+Herba Taraxaci+Chinese medicine, and (medicine assisted a ruler in governing a country by Chinese medicine is Fructus Mori+Radix Glycyrrhizae+hardship
Semen Fagopyri Esculenti, the proportioning of each composition and preparation method reference example 1).
D group: medicine assisted a ruler in governing a country by Herba Taraxaci+Fructus Hippophae+Chinese medicine, and (medicine assisted a ruler in governing a country by Chinese medicine is Fructus Mori+Radix Glycyrrhizae+Radix Et Rhizoma Fagopyri Tatarici
Wheat, the proportioning of each composition and preparation method reference example 1).
E group: patent group of the present invention, medicine assisted a ruler in governing a country by Fructus Hippophae+Herba Taraxaci+Radix Isatidis+Chinese medicine, and (Chinese medicine is assisted a ruler in governing a country
Medicine is Fructus Mori+Radix Glycyrrhizae+Radix Et Rhizoma Fagopyri Tatarici, the proportioning of each composition and preparation method reference example 1).
2, experiment material.
2.1 Chinese crude drugs: Fructus Hippophae, Herba Taraxaci, Radix Isatidis, Fructus Mori, Radix Glycyrrhizae and Radix Et Rhizoma Fagopyri Tatarici all purchase in
The big pharmacy of Beijing Tongrentang.
2.2 influenza virus: A/California F07/09 (H1N1), this strain virus is stream in 2009
Feel pandemic international representative strains.Preserved by Disease Control and Prevention Center of country virus.
2.3 cell strains: MDCK system Madin-Darby canine kidney(cell line) (MDCK), by the passed on preservation of virus of Disease Control and Prevention Center of country.
3, experimental technique:
According to document (Shu Liping, modern combination of Chinese and Western medicine magazine.35 phases of volume 18 in 2009:
4339-4340) method is tested.
3.1 influenza virus pass on the titration with hemagglutinative titer: influenza virus is inoculated in instar chicken embryo on the 9th
Allantoic cavity, cultivates 48h, collects allantoic fluid the most afterwards for 4 DEG C for 37 DEG C by 0.2mL/；Through hemagglutination test
Titration viral hemoagglutination titer is 1/1500, and 1500 × g is centrifuged 5 minutes, takes supernatant, tubule subpackage ,-20
DEG C save backup.
3.2 medicines toxic action to mdck cell: with the DMEM training dual anti-containing penicillin and streptomycin
After Fructus Hippophae, Herba Taraxaci and Radix Isatidis (400mg/mL) are made series two-fold dilution by nutrient solution, it is seeded in
On the MDCK cell monolayers of 1 age in days, each dilution factor inoculates 4 holes (0.1mL/ hole), hatches 2 for 37 DEG C
Hour, abandon supernatant, the cell growth status in each hole of Continuous Observation, and it is right to set DMEM culture fluid blank
According to.
The directly effect of 3.3 medicine infected by influenzas: with Hanks liquid by Fructus Hippophae, Herba Taraxaci and Baphicacanthus cusia
Root (25mg/ml) does doubling dilution, is separately added into equivalent 1500Hu (HAU) influenza virus,
37 DEG C of water-baths immediately, after acting on 8 hours, measure hemagglutinative titer respectively.
4, experimental result: through calculating, draw following result, be shown in Table 3 and Fig. 3:
Table 3: the direct suppression ratio of infected by influenza
| Directly suppression ratio | |
| A group | 21% |
| B group | 30% |
| C group | 58% |
| D group | 67% |
| E group | 100% |
Table 3 and Fig. 3 result shows:
1. five groups of A, B, C, D and E are the least to the toxicity of mdck cell, and cellular morphology is relatively
Good, grow vigorous.
2. [of the present invention group, medicine (Fructus Mori+Radix Glycyrrhizae assisted a ruler in governing a country by Fructus Hippophae+Herba Taraxaci+Radix Isatidis+Chinese medicine to E group
+ Radix Et Rhizoma Fagopyri Tatarici)] the direct suppression ratio of infected by influenza reached 100%；C group [Radix Isatidis+Herba Taraxaci+
Medicine (Fructus Mori+Radix Glycyrrhizae+Radix Et Rhizoma Fagopyri Tatarici) assisted a ruler in governing a country by Chinese medicine] suppression ratio be 58%；D group [(Herba Taraxaci+
Medicine (Fructus Mori+Radix Glycyrrhizae+Radix Et Rhizoma Fagopyri Tatarici) assisted a ruler in governing a country by Fructus Hippophae+Chinese medicine] suppression ratio 67%.And A group is (blank
Matched group) and the suppression ratio of B group (medicine assisted a ruler in governing a country by Chinese medicine) infected by influenza respectively be 21% and 30%.
E group (of the present invention group), compared with A, B, C, D tetra-groups, has significant differences (P < 0.01).
As can be seen here, Fructus Hippophae, Herba Taraxaci and Radix Isatidis compatibility, highly significant to enhance convection current susceptible
The inhibitory action of poison, can directly suppress the influenza virus infection to cell.
Experimental example 4: the Fructus Hippophae compound recipe impact on mouse influenza virus neutralizing antibody
1, experiment packet:
Using mice to test, this experiment is divided into 5 groups, is A, B, C, D and E respectively.
A group: blank group, is not added with any medicine.
B group: Chinese medicine assists a ruler in governing a country medicine (Fructus Mori+Radix Glycyrrhizae+Radix Et Rhizoma Fagopyri Tatarici, the proportioning of each composition and preparation method
Reference example 1).
C group: medicine assisted a ruler in governing a country by Radix Isatidis+Herba Taraxaci+Chinese medicine, and (medicine assisted a ruler in governing a country by Chinese medicine is Fructus Mori+Radix Glycyrrhizae+hardship
Semen Fagopyri Esculenti, the proportioning of each composition and preparation method reference example 1).
D group: medicine assisted a ruler in governing a country by Herba Taraxaci+Fructus Hippophae+Chinese medicine, and (medicine assisted a ruler in governing a country by Chinese medicine is Fructus Mori+Radix Glycyrrhizae+Radix Et Rhizoma Fagopyri Tatarici
Wheat, the proportioning of each composition and preparation method reference example 1).
E group: patent group of the present invention, medicine assisted a ruler in governing a country by Fructus Hippophae+Herba Taraxaci+Radix Isatidis+Chinese medicine, and (Chinese medicine is assisted a ruler in governing a country
Medicine is Fructus Mori+Radix Glycyrrhizae+Radix Et Rhizoma Fagopyri Tatarici, the proportioning of each composition and preparation method reference example 1).
2, experiment material.
2.1 Chinese crude drugs: Fructus Hippophae, Herba Taraxaci, Radix Isatidis, Fructus Mori, Radix Glycyrrhizae and Radix Et Rhizoma Fagopyri Tatarici all purchase in
The big pharmacy of Beijing Tongrentang.
2.2 influenza virus: Flu virus A/FM/1/47 (H1N1).By country's disease control
Middle Cardioviruses is preserved.
2.3 cell strains: MDCK system Madin-Darby canine kidney(cell line) (MDCK), by the passed on preservation of virus of Disease Control and Prevention Center of country.
2.4 laboratory animals: cleaning grade Bal b/c mice, body weight 18-20g, male and female half and half, by Beijing
Medical university's Animal House provides.
3, experimental technique:
According to document (Zheng Qun etc., Capital University of Medical Sciences's journal.Volume 25 2 phases: 180-182 in 2004)
(Shu Liping, modern combination of Chinese and Western medicine magazine.Volume 18 35 phases: 4339-4340 in 2009) method
Test.
Passing on of 3.1 influenza virus: with experimental example 1.
The detection of 3.2 mice neutralizing antibodies.
Take mice 40, with normal saline, virus liquid is diluted to mice median lethal dose(LD 50)
(LD50=10-3.25) 15 times, with the influenza virus collunarium infecting mouse of this titre.
Mice is divided into A, B, C, D, E five groups, often group 10.Use influenza infection mice
After 2 days, it is real that medicine (Fructus Mori+Radix Glycyrrhizae+Radix Et Rhizoma Fagopyri Tatarici) assisted a ruler in governing a country by Fructus Hippophae, Herba Taraxaci, Radix Isatidis and Chinese medicine
Test dosage and use minimum non-toxic 25mg/ml, according to five groups of A, B, C, D and E, respectively
Oral administration 5 days, the 6th day starts to detect the NAT of mice.Detection neutralizing antibody uses
Cell be mdck cell；Eyeball of mouse blood-letting is lethal, separates determination of serum neutralizing antibody.
4, experimental result: according to testing result, by calculating, draw following result, be specifically shown in Table 4
And Fig. 4.
Table 4: the impact on NAT
| NAT | |
| A group | 1:180 |
| B group | 1:185 |
| C group | 1:310 |
| D group | 1:520 |
| E group | 1:720 |
Table 4 and Fig. 4 result shows:
A group is the blank group of comparison, and the NAT of mice is 1:180；B group (Chinese medicine adjuvant
Thing), the NAT of mice is 1:185；C group (medicine assisted a ruler in governing a country by Radix Isatidis+Herba Taraxaci+Chinese medicine),
The NAT of mice is 1:310；D group (medicine assisted a ruler in governing a country by Herba Taraxaci+Fructus Hippophae+Chinese medicine), mice
NAT be 1:520；(of the present invention group, Fructus Hippophae+Herba Taraxaci+Radix Isatidis+Chinese medicine is auxiliary for E group
Adjuvant drug thing), the neutralization titre of mice is 1:720.Visible, E group compared with A, B, C, D tetra-groups,
There is significant differences (P < 0.01).
As can be seen here, Fructus Hippophae, Herba Taraxaci and the Radix Isatidis three's compatibility that the present invention uses, significantly carry
The high neutralizing antibody reaction of mice against influenza virus, i.e. by the immunologic function in regulation Mice Body,
Improve the immunity of mice, strengthen the mice resistance to pathogenic bacteria.
Although, used general explanation, detailed description of the invention and test, the present invention made
Detailed description, but on the basis of the present invention, it can be made some modifications or improvements, this is to this
It is apparent from for skilled person.Therefore, institute without departing from theon the basis of the spirit of the present invention
The these modifications or improvements done, belong to the scope of protection of present invention.
Claims (10)
1. the Fructus Hippophae compound recipe preventing and treating influenza, it is characterised in that it includes following component: Fructus Hippophae,
Herba Taraxaci, Radix Isatidis.
Fructus Hippophae compound recipe the most according to claim 1, it is characterised in that described Fructus Hippophae compound recipe includes
The composition of following weight portion: Fructus Hippophae 2-80 part, Herba Taraxaci 2-40 part, Radix Isatidis 4-80 part.
Fructus Hippophae compound recipe the most according to claim 2, it is characterised in that described Fructus Hippophae method includes
The composition of following weight portion: Fructus Hippophae 20-60 part, Herba Taraxaci 10-30 part, Radix Isatidis 15-45 part.
Fructus Hippophae compound recipe the most according to claim 3, it is characterised in that described Fructus Hippophae method includes
The composition of following weight portion: Fructus Hippophae 40 parts, Herba Taraxaci 20 parts, Radix Isatidis 30 parts.
5. according to the Fructus Hippophae compound recipe described in any one of claim 1-4, it is characterised in that possibly together with following
Composition: Fructus Mori, Radix Glycyrrhizae and Radix Et Rhizoma Fagopyri Tatarici, the weight portion of Fructus Mori, Radix Glycyrrhizae and Radix Et Rhizoma Fagopyri Tatarici is: Fructus Mori 1-20
Part, Radix Glycyrrhizae 1-20 part and Radix Et Rhizoma Fagopyri Tatarici 1-20 part.
Fructus Hippophae compound recipe the most according to claim 5, it is characterised in that in compound recipe, Fructus Mori, sweet
The weight portion of grass and Radix Et Rhizoma Fagopyri Tatarici is: Fructus Mori 1-10 part, Radix Glycyrrhizae 1-8 part and Radix Et Rhizoma Fagopyri Tatarici 1-8 part.
Fructus Hippophae compound recipe the most according to claim 6, it is characterised in that in compound recipe, Fructus Mori, sweet
The weight portion of grass and Radix Et Rhizoma Fagopyri Tatarici is: Fructus Mori 4 parts, 3 parts of Radix Glycyrrhizae and Radix Et Rhizoma Fagopyri Tatarici 3 parts.
8. a preparation, it is characterised in that its by the Fructus Hippophae compound recipe described in any one of claim 1-7 and
Pharmaceutically acceptable carrier forms.
9. the preparation method of the Fructus Hippophae compound recipe described in any one of claim 1-7, the method includes following step
Rapid:
Fructus Hippophae, Fructus Mori, Herba Taraxaci, Radix Glycyrrhizae, Radix Et Rhizoma Fagopyri Tatarici, Radix Isatidis is weighed according to proportioning, standby；
Respectively Radix Glycyrrhizae, Radix Et Rhizoma Fagopyri Tatarici, Radix Isatidis are pulverized, add water after mixing, at a temperature of 65-100 DEG C
Extract 1-10 hour, obtain water extract；
Respectively by Fructus Hippophae, Fructus Mori and Herba Taraxaci lyophilization, grind to form fine powder, add above-mentioned water logging
Body fluid, to obtain final product.
10. the Fructus Hippophae compound recipe described in any one of claim 1-7 or the preparation described in claim 8 are in system
Application in the medicine of standby prevention and treatment influenza virus.
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| CN2014104473964 | 2014-09-04 | ||
| CN201410447396.4A CN104173460A (en) | 2014-09-04 | 2014-09-04 | Compound sea-buckthorn granule for curing influenza |
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| CN107041910A (en) * | 2017-04-15 | 2017-08-15 | 王秀梅 | A kind of compound cold granule |
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Non-Patent Citations (2)
| Title |
|---|
| 盛晓静: "复方公英片质量标准的研究", 《中成药》 * |
| 赵改过: "复感汤合沙棘干乳剂治疗反复呼吸道感染96例", 《陕西中医》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107041910A (en) * | 2017-04-15 | 2017-08-15 | 王秀梅 | A kind of compound cold granule |
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