CN106170560A - 下一代基因组测序方法 - Google Patents

下一代基因组测序方法 Download PDF

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Publication number
CN106170560A
CN106170560A CN201480071698.4A CN201480071698A CN106170560A CN 106170560 A CN106170560 A CN 106170560A CN 201480071698 A CN201480071698 A CN 201480071698A CN 106170560 A CN106170560 A CN 106170560A
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CN
China
Prior art keywords
primer
sequence
gene
nucleic acid
virus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480071698.4A
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English (en)
Chinese (zh)
Inventor
L.T.道姆
G.W.费希尔
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Longhorn Vaccines and Diagnostics LLC
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Longhorn Vaccines and Diagnostics LLC
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Publication date
Application filed by Longhorn Vaccines and Diagnostics LLC filed Critical Longhorn Vaccines and Diagnostics LLC
Publication of CN106170560A publication Critical patent/CN106170560A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201480071698.4A 2013-10-29 2014-10-29 下一代基因组测序方法 Pending CN106170560A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361897015P 2013-10-29 2013-10-29
US61/897015 2013-10-29
PCT/US2014/062889 WO2015066174A1 (fr) 2013-10-29 2014-10-29 Méthodes de séquençage génomique de nouvelle génération

Publications (1)

Publication Number Publication Date
CN106170560A true CN106170560A (zh) 2016-11-30

Family

ID=53005068

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480071698.4A Pending CN106170560A (zh) 2013-10-29 2014-10-29 下一代基因组测序方法

Country Status (5)

Country Link
EP (1) EP3063301A4 (fr)
CN (1) CN106170560A (fr)
AU (1) AU2014342414B2 (fr)
CA (1) CA2929108A1 (fr)
WO (1) WO2015066174A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112236526A (zh) * 2018-04-20 2021-01-15 长角牛疫苗和诊断有限责任公司 用于检测微生物抗性的快速方法
CN112410406A (zh) * 2020-11-23 2021-02-26 深圳基因家科技有限公司 一种确定文库扩增循环数的方法

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11041216B2 (en) 2007-10-01 2021-06-22 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
GB2533173A (en) 2013-08-05 2016-06-15 Twist Bioscience Corp De Novo synthesized gene libraries
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
IL258164B (en) 2015-09-18 2022-09-01 Twist Bioscience Corp Methods to regulate the activity of proteins and cells and a method for the production of nucleic acids
KR20180058772A (ko) 2015-09-22 2018-06-01 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
WO2017091809A1 (fr) * 2015-11-25 2017-06-01 Longhorn Vaccines And Diagnostics, Llc Compositions et procédés de détection et de quantification de séquences d'acides nucléiques dans des échantillons sanguins
CN105950726A (zh) * 2016-05-16 2016-09-21 杭州市疾病预防控制中心 结核分枝杆菌吡嗪酰胺分子药敏的检测方法
JP6854340B2 (ja) 2016-08-22 2021-04-07 ツイスト バイオサイエンス コーポレーション デノボ合成された核酸ライブラリ
JP6871364B2 (ja) 2016-09-21 2021-05-12 ツイスト バイオサイエンス コーポレーション 核酸に基づくデータ保存
WO2018094263A1 (fr) * 2016-11-18 2018-05-24 Twist Bioscience Corporation Banques de polynucléotides à stœchiométrie contrôlée et leur procédé de synthèse
CA3054303A1 (fr) 2017-02-22 2018-08-30 Twist Bioscience Corporation Stockage de donnees reposant sur un acide nucleique
CN110913865A (zh) 2017-03-15 2020-03-24 特韦斯特生物科学公司 免疫突触的变体文库及其合成
AU2018284227B2 (en) 2017-06-12 2024-05-02 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (fr) 2017-06-12 2018-12-20 Twist Bioscience Corporation Méthodes d'assemblage d'acides nucléiques continus
EA202090562A1 (ru) 2017-09-11 2020-08-10 Твист Байосайенс Корпорейшн Связывающие gpcr белки и их синтез
US10368502B2 (en) 2017-09-25 2019-08-06 Multiple Energy Technologies Llc Bioceramic and carbon-based hydroponic systems, methods and devices
US10894242B2 (en) 2017-10-20 2021-01-19 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
EP3735459A4 (fr) 2018-01-04 2021-10-06 Twist Bioscience Corporation Stockage d'informations numériques reposant sur l'adn
SG11202011467RA (en) 2018-05-18 2020-12-30 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
SG11202109283UA (en) 2019-02-26 2021-09-29 Twist Bioscience Corp Variant nucleic acid libraries for antibody optimization
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CN112779343A (zh) * 2019-11-07 2021-05-11 杭州迪安医学检验中心有限公司 一种病原微生物药敏检测方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102250924A (zh) * 2010-05-17 2011-11-23 中国疾病预防控制中心病毒病预防控制所 盖塔病毒全基因组序列、扩增方法及引物
WO2012037531A1 (fr) * 2010-09-16 2012-03-22 Gen-Probe Incorporated Sondes de capture immobilisables par l'intermédiaire d'une queue nucléotidique l
CN104508142A (zh) * 2012-05-09 2015-04-08 长角牛疫苗和诊断有限责任公司 离子激流基因组测序

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8652782B2 (en) * 2006-09-12 2014-02-18 Longhorn Vaccines & Diagnostics, Llc Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
WO2013022961A1 (fr) * 2011-08-08 2013-02-14 3The Broad Institute Compositions et procédés pour la co-amplification de sous-séquences d'une séquence de fragment d'acide nucléique

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102250924A (zh) * 2010-05-17 2011-11-23 中国疾病预防控制中心病毒病预防控制所 盖塔病毒全基因组序列、扩增方法及引物
WO2012037531A1 (fr) * 2010-09-16 2012-03-22 Gen-Probe Incorporated Sondes de capture immobilisables par l'intermédiaire d'une queue nucléotidique l
CN104508142A (zh) * 2012-05-09 2015-04-08 长角牛疫苗和诊断有限责任公司 离子激流基因组测序
CN108374042A (zh) * 2012-05-09 2018-08-07 长角牛疫苗和诊断有限责任公司 离子激流基因组测序

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LANSING M. PRESCOTT等: "《微生物学》", 31 July 2003, 北京：高等教育出版社 *
LUKE T. DAUM等: ""Next-Generation Ion Torrent Sequencing of Drug Resistance Mutations in Mycobacterium tuberculosis Strains"", 《JOURNAL OF CLINICAL MICROBIOLOGY》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112236526A (zh) * 2018-04-20 2021-01-15 长角牛疫苗和诊断有限责任公司 用于检测微生物抗性的快速方法
CN112410406A (zh) * 2020-11-23 2021-02-26 深圳基因家科技有限公司 一种确定文库扩增循环数的方法

Also Published As

Publication number Publication date
WO2015066174A1 (fr) 2015-05-07
AU2014342414B2 (en) 2017-06-29
EP3063301A1 (fr) 2016-09-07
CA2929108A1 (fr) 2015-05-07
AU2014342414A1 (en) 2016-05-19
EP3063301A4 (fr) 2017-07-19

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Application publication date: 20161130