CN106170550A - 用于产生导引rna的方法和组合物 - Google Patents
用于产生导引rna的方法和组合物 Download PDFInfo
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- CN106170550A CN106170550A CN201580018277.XA CN201580018277A CN106170550A CN 106170550 A CN106170550 A CN 106170550A CN 201580018277 A CN201580018277 A CN 201580018277A CN 106170550 A CN106170550 A CN 106170550A
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- nucleotide sequence
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- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2330/00—Production
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- C12N2330/51—Specially adapted vectors
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- C12N2830/00—Vector systems having a special element relevant for transcription
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- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
- C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461974672P | 2014-04-03 | 2014-04-03 | |
| US61/974,672 | 2014-04-03 | ||
| PCT/US2015/024196 WO2015153940A1 (en) | 2014-04-03 | 2015-04-03 | Methods and compositions for the production of guide rna |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106170550A true CN106170550A (zh) | 2016-11-30 |
Family
ID=52997563
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580018277.XA Pending CN106170550A (zh) | 2014-04-03 | 2015-04-03 | 用于产生导引rna的方法和组合物 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20170022499A1 (https=) |
| EP (1) | EP3126503A1 (https=) |
| JP (1) | JP2017509350A (https=) |
| CN (1) | CN106170550A (https=) |
| WO (1) | WO2015153940A1 (https=) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728441A (zh) * | 2018-04-18 | 2018-11-02 | 深圳市第二人民医院 | 特异性识别p53突变的基因系统 |
| CN114829607A (zh) * | 2019-12-18 | 2022-07-29 | 因思科瑞普特公司 | 用于体内检测核酸引导的核酸酶编辑的细胞的Cascade/dCas3互补测定 |
| WO2023011659A1 (zh) * | 2021-08-06 | 2023-02-09 | 华东理工大学 | 基于CRISPRi和CRISPRa的转录调控系统、其建立方法及应用 |
| CN119220682A (zh) * | 2024-10-25 | 2024-12-31 | 首都医科大学附属北京儿童医院 | Saal1作为神经母细胞瘤预后评估标志物和药物靶点的应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| CN112301024A (zh) | 2013-03-15 | 2021-02-02 | 通用医疗公司 | 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性 |
| WO2014204578A1 (en) | 2013-06-21 | 2014-12-24 | The General Hospital Corporation | Using rna-guided foki nucleases (rfns) to increase specificity for rna-guided genome editing |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| WO2015066119A1 (en) | 2013-10-30 | 2015-05-07 | North Carolina State University | Compositions and methods related to a type-ii crispr-cas system in lactobacillus buchneri |
| CN106459995B (zh) | 2013-11-07 | 2020-02-21 | 爱迪塔斯医药有限公司 | 使用统治型gRNA的CRISPR相关方法和组合物 |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| CA2935032C (en) * | 2013-12-26 | 2024-01-23 | The General Hospital Corporation | Multiplex guide rnas |
| KR20160128306A (ko) * | 2014-01-14 | 2016-11-07 | 램 테라퓨틱스, 인코포레이티드 | 돌연변이유발 방법 |
| US10787654B2 (en) | 2014-01-24 | 2020-09-29 | North Carolina State University | Methods and compositions for sequence guiding Cas9 targeting |
| JP2017512481A (ja) | 2014-04-08 | 2017-05-25 | ノースカロライナ ステート ユニバーシティーNorth Carolina State University | Crispr関連遺伝子を用いた、rna依存性の転写抑制のための方法および組成物 |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| EP3186375A4 (en) | 2014-08-28 | 2019-03-13 | North Carolina State University | Novel CAS9 PROTEINS AND CHARACTERISTICS FOR DNA TARGETING AND GENOME EDITING |
| TWI716367B (zh) * | 2014-10-31 | 2021-01-21 | 麻省理工學院 | 用於常間回文重複序列叢集(crispr)之大量平行組合性基因學 |
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| JP6529110B2 (ja) * | 2014-12-01 | 2019-06-12 | 国立大学法人 東京大学 | 複数のユニットが多重に連結したdnaカセットおよび該カセットを含むベクターの製造方法 |
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| WO2016205276A1 (en) | 2015-06-15 | 2016-12-22 | North Carolina State University | Methods and compositions for efficient delivery of nucleic acids and rna-based antimicrobials |
| US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| US9926546B2 (en) | 2015-08-28 | 2018-03-27 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| EP3356533A1 (en) | 2015-09-28 | 2018-08-08 | North Carolina State University | Methods and compositions for sequence specific antimicrobials |
| IL310721B2 (en) | 2015-10-23 | 2025-11-01 | Harvard College | Nucleobase editors and uses thereof |
| WO2017106414A1 (en) * | 2015-12-18 | 2017-06-22 | Danisco Us Inc. | Methods and compositions for polymerase ii (pol-ii) based guide rna expression |
| US11542466B2 (en) | 2015-12-22 | 2023-01-03 | North Carolina State University | Methods and compositions for delivery of CRISPR based antimicrobials |
| CN105543223A (zh) * | 2015-12-25 | 2016-05-04 | 华侨大学 | 一种基于miRNA/shRNA转录加工机制转录sgRNA的方法 |
| EP3199632A1 (en) | 2016-01-26 | 2017-08-02 | ACIB GmbH | Temperature-inducible crispr/cas system |
| EP3219799A1 (en) | 2016-03-17 | 2017-09-20 | IMBA-Institut für Molekulare Biotechnologie GmbH | Conditional crispr sgrna expression |
| JP2019514377A (ja) | 2016-04-26 | 2019-06-06 | マサチューセッツ インスティテュート オブ テクノロジー | 拡張可能なリコンビナーゼカスケード |
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| EP3126503A1 (en) | 2017-02-08 |
| JP2017509350A (ja) | 2017-04-06 |
| US20170022499A1 (en) | 2017-01-26 |
| WO2015153940A1 (en) | 2015-10-08 |
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