CN106138206A - A kind of plant type milch cow special uterus washing liquid and preparation method thereof - Google Patents
A kind of plant type milch cow special uterus washing liquid and preparation method thereof Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
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Abstract
The invention discloses a kind of plant type milch cow special uterus washing liquid, plant extract is adsorbed by the attapulgite utilizing activation, it is made into lotion, wherein, described plant extract is mainly composed of Herba Sophorae alopecuroidis alkali, Flos Lonicerae extract, Herba Hyperici perforati extract, Radix Scutellariae extract, Herba Leonuri extract Flos Carthami extract.Herba Sophorae alopecuroidis, Flos Lonicerae, Herba Hyperici perforati, Radix Scutellariae, Herba Leonuri, Flos Carthami extract through rational formula and manufacturing process, are not only remained good bactericidal effect with attapulgite by the application, and can be as milch cow special uterus lotion.Ensure milk cow health, there is preferable range of application.
Description
Technical field
The present invention relates to a kind of plant type milch cow special uterus washing liquid and preparation method thereof.
Background technology
The inflammation of the endometrium that cow endometritis is milch cow generally to be occurred in puerperal, it continues development and just becomes son
Cervicitis and pyometritis, endometritis and metritis often mix generation clinically.This disease is scorching on the impact foundation of breeding
Time that the degree of disease, endometrial impairment recover to need, the damage of endometrial gland, fallopian tube environment change different and
Different.Endometritis makes calving interval prolongation, milk yield reduce, mortality increases, medical expense increases and antibiotic therapy
Period milk is discarded, and causes the biggest economic loss, and it is 106 dollars that the lactation period of external each infected cattle spends.This disease is to lead
Cause the infertile one of the main reasons of milch cow, abroad report calver does not has 70% for chronic endometritis.At home, milk Fructus Arctii
The sickness rate of Endometrium inflammation is the highest, and the result of 41 the cattle farm investigation in 16 cities is shown by 1983-1985, cow uteri
Intimitis sickness rate is 17.26%, accounts for the 68.34% of not calver.As can be seen here, the economic damage that cow endometritis is caused
Mistake is the most serious.China veterinary scientific worker, according to Chinese veterinarian's pharmacology opinion, prevents and treats cow endometritis, achieves
Gratifying progress, different researcheres, according to problem present in clinic, use different Chinese herbal treatment methods, as oral
Chinese medicine formula, makes Chinese herbal medicine decoction and carries out intrauterine infusion, makes pill uterus dispensing etc., and Chinese herbal compound preparation
Development and Chinese herbal medicine bacteriostatic test research etc..
Summary of the invention
The technical problem to be solved in the present invention is to overcome existing defect, it is provided that a kind of non-stimulated, slow release, plant efficiently
Thing type milch cow special uterus washing liquid；
It is a further object of the present invention to provide the preparation method of above-mentioned plant type milch cow special uterus washing liquid.
The purpose of the present invention implements by the following technical programs:
A kind of plant type milch cow special uterus washing liquid, utilizes the attapulgite of activation to adsorb plant extract, is made into
Lotion,
Wherein, in described plant extract be mainly composed of Herba Sophorae alopecuroidis alkali, Flos Lonicerae extract, Herba Hyperici perforati extract
Thing, Radix Scutellariae extract, Herba Leonuri extract Flos Carthami extract.
Preferably, above-mentioned plant type milch cow special uterus washing liquid, each component by weight: Herba Sophorae alopecuroidis alkali 5-40 part,
Flos Lonicerae extract 10-25 part, Herba Hyperici perforati extract 5-20 part, Radix Scutellariae extract 10-25 part, Herba Leonuri extract 20-35
Part, Flos Carthami extract 10-25 part, attapulgite 2-5 part；As preferably, the mass parts of Herba Sophorae alopecuroidis alkali is 5-20 part.
It is further preferred that above-mentioned plant type milch cow special uterus washing liquid, each component by weight: Herba Sophorae alopecuroidis alkali 15
Part, Flos Lonicerae extract 15 parts, Herba Hyperici perforati extract 10 parts, Radix Scutellariae extract 15 parts, Herba Leonuri extract 25 parts, Flos Carthami carry
Take thing 15 parts, attapulgite 3 parts.
Preferably, in described Herba Sophorae alopecuroidis alkali, total alkali is 30%；Chlorogenic acid content in described Flos Lonicerae extract is
20%；Determination of Hypericin from Extraction in described Herba Hyperici perforati extract is 0.3%；
Described Radix Scutellariae extract is the extract extracting with the former grass of 10 weight portions and obtaining 1 weight portion；
Described Herba Leonuri extract is the extract extracting with the former grass of 10 weight portions and obtaining 1 weight portion；
Described Flos Carthami extract is the extract extracting with the former grass of 20 weight portions and obtaining 1 weight portion.
The preparation method of above-mentioned 1000ml plant type milch cow special uterus washing liquid is,
1) weighing attapulgite 2-5g by proportioning, add 100-250ml sour water and boil 30 minutes, making pH is 3, after placing room temperature
Regulation pH is 7, boils to remove wherein solubility and volatile impurity in pure water, filters precipitation, at 100 DEG C of oven dryings
2h, then activate 2h at 500 DEG C, obtain active attapulgite；
2) plant extract taking proportional quantity adds pure water dilution, makes mix homogeneously；
3) add step 1) active attapulgite for preparing, after stirring at normal temperature 30min, add tween 80,1,3-third
Glycol；
4) pure water is added to 1000ml；
5) stirring at normal temperature 30min；
6) subpackage, high temperature sterilize, obtain washing liquid.
Preferably, described step 1) in place after room temperature the sodium hydroxide solution regulation pH with 5% be 7；Pure water boils
1h。
Preferably, described step 3) in add amount is washing liquid weight the 10% of tween 80, the amount of 1,3-PD is washing liquid
The 5% of weight.
The present invention also provides for the above-mentioned plant type milch cow special uterus washing liquid medicine at preparation treatment cow endometritis
In application.
The present invention also provides for a kind of medicine, and the effective ingredient of described medicine is that the plant type milch cow described in any of the above-described item is special
Use uterus washing liquid.
The present invention is with Herba Sophorae alopecuroidis alkaline extraction thing, Flos Lonicerae extract, Herba Hyperici perforati extract, Radix Scutellariae extract, Herba Leonuri
Extract, Flos Carthami extract are combined the milch cow lotion special of preparation with attapulgite.Attapulgite clay refers to attapulgite
A kind of clay mineral for key component.Attapulgite is a kind of crystalloid hydrous magnesium aluminium silicate mineral, has the layer chain of uniqueness
, in its structure, there is crystal lattice, containing variable amount of Na in side crystal in shape architectural feature+、Ca2+、Fe3+、Al3+, crystal in
Needle-like, threadiness or fiber collection shape.Attapulgite has the dispersion of uniqueness, the good colloidal nature such as saline and alkaline high temperature resistant, anti-
Higher absorbability.Due to the crystal structure that attapulgite is unique, it is allowed to that there is many special materializations and processing performance.
Main physical and chemical performance and processing performance have: cation interchangeability, adsorptivity, adsorption bleaching, big specific surface area (9.6～
36m2/ g) and colloid index and expansion capacity.These character can make cavity of uterus contact area and time lengthening, zest significantly under
Fall, its stability is also greatly enhanced simultaneously, and has repair to film slough tissue in cavity of uterus body, therefore improves its sterilization
Effectiveness, along with the development of automatic cleaning system, non-stimulated, slow release, efficient antibacterial have become milch cow obstetrics preferably makes
Use medicine.
Herba Sophorae alopecuroidis, Flos Lonicerae, Herba Hyperici perforati, Radix Scutellariae, Herba Leonuri, Flos Carthami extract are passed through with attapulgite and close by the application
The formula of reason and manufacturing process, not only remain good bactericidal effect, and can be as milch cow special uterus lotion.Ensure milch cow
Health, has preferable range of application.
The application products characteristics is as follows:
1, feature: this product not only has the feature of general uterus lotion, effects such as active adsorption abnormal flavour of also having are used for milch cow
Hysteropathy preventing and treating.
2, safety: this product is used for nonirritant, safe and reliable.
3, environmental protection: this product is without any side effects, does not cause any pollution, environmental protection to environment.
4, wide spectrum: according to the testing result to this product, the pathogenic microorganisms such as virus and bacteria are respectively provided with and significantly kill by this product
The effect of going out.
5, efficient: certain density this product solution 100% can kill pathogenic microorganism.
Detailed description of the invention
Hereinafter the preferred embodiments of the present invention are illustrated, it will be appreciated that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
Preparation example 1:
The preparation method of plant washing liquid (1000ml), wherein, including following raw material components:
1. Herba Sophorae alopecuroidis alkali (Ningxia Flos cercis chinensis pharmaceutcal corporation, Ltd total alkali 30%): 20g
2. Flos Lonicerae extract (Shaanxi Zhong Xin Bioisystech Co., Ltd, chlorogenic acid 20%): 10g
3. Herba Hyperici perforati extract (Shaanxi Jin Tai Bioisystech Co., Ltd, hypericin 0.3%): 5g
4. Radix Scutellariae extract (Shaanxi Zhong Xin Bioisystech Co., Ltd, 10:1): 10g
5. Herba Leonuri extract (bright biological development corporation, Ltd. of Baoji side, 10:1): 20g
6. Flos Carthami extract (bright biological development corporation, Ltd. of Baoji side, 20:1): 10g
7. attapulgite (Jingyuan, Gansu county Hao Di industry and trade Co., Ltd): 2g.
Comprise the concrete steps that:
1) attapulgite is weighed as 4.0g, adds and boils 30 minutes (hydrochloric acid adjusts pH to be 3) in 100ml sour water, after placing room temperature
It is adjusted to neutrality (pH is 7) with the sodium hydroxide solution of 5%, in pure water, boils 1h miscellaneous to remove wherein solubility and volatility
Matter；Filter precipitation, at 100 DEG C of oven drying 2h, then activate 2h at 500 DEG C, obtain active attapulgite；
2) take each Plant Extracts of above-mentioned formula ratio and in 2000ml conical flask, add pure water respectively to 300ml；
3) active attapulgite is put into step 2) conical flask in, after stirring at normal temperature 30min, add 100ml tween 80 and
The 1,3-propylene glycol of 50ml；
4) pure water is added to 1000ml；
6) stirring at normal temperature 30min；
7) subpackage, high temperature sterilize.
Preparation example 2:
The preparation method of plant washing liquid (1000ml), wherein, including following raw material components:
1. Herba Sophorae alopecuroidis alkali (Ningxia Flos cercis chinensis pharmaceutcal corporation, Ltd total alkali 30%): 15g
2. Flos Lonicerae extract (Shaanxi Zhong Xin Bioisystech Co., Ltd, chlorogenic acid 20%): 15g
3. Herba Hyperici perforati extract (Shaanxi Jin Tai Bioisystech Co., Ltd, hypericin 0.3%): 10g
4. Radix Scutellariae extract (Shaanxi Zhong Xin Bioisystech Co., Ltd, 10:1): 15g
5. Herba Leonuri extract (bright biological development corporation, Ltd. of Baoji side, 10:1): 25g
6. Flos Carthami extract (bright biological development corporation, Ltd. of Baoji side, 20:1): 15g
7. attapulgite (Jingyuan, Gansu county Hao Di industry and trade Co., Ltd) 3g.
Comprise the concrete steps that:
1) attapulgite is weighed as 3.0g, adds and boils 30 minutes (hydrochloric acid adjusts pH to be 3) in 150ml sour water, after placing room temperature
It is adjusted to neutrality (pH is 7) with the sodium hydroxide solution of 5%, in pure water, boils 1h miscellaneous to remove wherein solubility and volatility
Matter, filters precipitation, at 100 DEG C of oven drying 2h, then activates 2h at 500 DEG C, obtains active attapulgite；
2) take each Plant Extracts of above-mentioned amount and in 2000ml conical flask, add pure water respectively to 300ml；
3) active attapulgite is put into step 2) conical flask in, after stirring at normal temperature 30min, add 100ml tween 80 and
The 1,3-propylene glycol of 50ml；
4) pure water is added to 1000ml；
6) stirring at normal temperature 30min；
7) subpackage, high temperature sterilize.
Preparation example 3:
The preparation method of plant washing liquid (1000ml), wherein, including following raw material components:
1. Herba Sophorae alopecuroidis alkali (Ningxia Flos cercis chinensis pharmaceutcal corporation, Ltd total alkali 30%): 10g
2. Flos Lonicerae extract (Shaanxi Zhong Xin Bioisystech Co., Ltd, chlorogenic acid 20%): 20g
3. Herba Hyperici perforati extract (Shaanxi Jin Tai Bioisystech Co., Ltd, hypericin 0.3%): 15g
4. Radix Scutellariae extract (Shaanxi Zhong Xin Bioisystech Co., Ltd, 10:1): 20g
5. Herba Leonuri extract (bright biological development corporation, Ltd. of Baoji side, 10:1): 30g
6. Flos Carthami extract (bright biological development corporation, Ltd. of Baoji side, 20:1): 20g
7. attapulgite (Jingyuan, Gansu county Hao Di industry and trade Co., Ltd) 4g.
Comprise the concrete steps that:
1) attapulgite is weighed as 5.0g, adds and boils 30 minutes (hydrochloric acid adjusts pH to be 3) in 200ml sour water, after placing room temperature
It is adjusted to neutrality (pH is 7) with the sodium hydroxide solution of 5%, in pure water, boils 1h miscellaneous to remove wherein solubility and volatility
Matter；Filter precipitation, at 100 DEG C of oven drying 2h, then activate 2h at 500 DEG C, obtain active attapulgite；
2) take each Plant Extracts of above-mentioned formula ratio and in 2000ml conical flask, add pure water respectively to 300ml；
3) active attapulgite is put into step 2) conical flask in, after stirring at normal temperature 30min, add 100ml tween 80 and
The 1,3-propylene glycol of 50ml；
4) pure water is added to 1000ml；
6) stirring at normal temperature 30min；
7) subpackage, high temperature sterilize.
Preparation example 4:
The preparation method of plant washing liquid (1000ml), wherein, including following raw material components:
1. Herba Sophorae alopecuroidis alkali (Ningxia Flos cercis chinensis pharmaceutcal corporation, Ltd total alkali 30%): 5g
2. Flos Lonicerae extract (Shaanxi Zhong Xin Bioisystech Co., Ltd, chlorogenic acid 20%): 25g
3. Herba Hyperici perforati extract (Shaanxi Jin Tai Bioisystech Co., Ltd, hypericin 0.3%): 20g
4. Radix Scutellariae extract (Shaanxi Zhong Xin Bioisystech Co., Ltd, 10:1): 25g
5. Herba Leonuri extract (bright biological development corporation, Ltd. of Baoji side, 10:1): 35g
6. Flos Carthami extract (bright biological development corporation, Ltd. of Baoji side, 20:1): 25g
7. attapulgite (Jingyuan, Gansu county Hao Di industry and trade Co., Ltd) 5g.
Comprise the concrete steps that:
1) attapulgite is weighed as 5.0g, adds and boils 30 minutes (hydrochloric acid adjusts pH to be 3) in 250ml sour water, after placing room temperature
It is adjusted to neutrality (pH is 7) with the sodium hydroxide solution of 5%, in pure water, boils 1h miscellaneous to remove wherein solubility and volatility
Matter；Filter precipitation, at 100 DEG C of oven drying 2h, then activate 2h at 500 DEG C, obtain active attapulgite；
2) take each Plant Extracts of above-mentioned formula ratio and in 2000ml conical flask, add pure water respectively to 300ml；
3) active attapulgite is put into step 2) conical flask in, after stirring at normal temperature 30min, add 100ml tween 80 and
The 1,3-propylene glycol of 50ml；
4) pure water is added to 1000ml；
6) stirring at normal temperature 30min；
7) subpackage, high temperature sterilize.
Test example 1:
The present invention is combined with attapulgite with Herba Sophorae alopecuroidis, Flos Lonicerae, Fructus Forsythiae, Radix Scutellariae, Radix Angelicae Sinensis, Herba Leonuri, Flos Carthami extract, system
Standby a kind of milch cow lotion special.This washing liquid has the effects such as heat-clearing and toxic substances removing, dispeiling pathogenic wind and removing dampness, pain relieving parasite killing, in vivo maincenter god
Through the aspect such as system, cardiovascular system and antiinflammatory, antiviral, antitumor, there is important pharmacologically active and application future.This examination
Testing to verify the effects such as its antibacterial, toxicity, the application being simultaneously this novel formulation by the result of this test provides clinical practice
Basic data.
1 method
1.1 nertralizer Selection experiment
The washing liquid stock solution of the test present invention, with escherichia coli and Candida albicans for nertralizer identification experiment bacterial strain.Examination
Test point 11 groups of (table) l, test by carrier quantitative germicidal test method.As a result, 9,10,11 groups of bacterium the longest, 1 group of bacterium the longest or
Being considerably less than the 2nd group, between 3,4,5 groups of groups, bacterial population difference is less than 10%, the 2nd group leader's bacterium number > 100cuf/ sheet time, in showing
Mediating recipe and concentration thereof are suitable.
1.2 carrier quantitative germicidal test
Test bacterial strain is escherichia coli (8099), staphylococcus aureus (ATCC6538), Candida albicans
(ATCC10231), Diplococcus gonorrhoeae (29106-6), be 24h fresh cultured thing.Under the conditions of 18-22 DEG C of water bath with thermostatic control, will
Bacterium sheet immerses in the plate of the washing liquid filling the present invention, acts on to the stipulated time, takes out bacterium sheet and is put in the examination filling 5ml nertralizer
Guan Zhong, neutralization 10min, beat test tube 250 times, with the antibacterial on eluting bacterium sheet and mix, takes eluent 0.5ml, pours into
Ordinary nutrient agar (Candida albicans is sabouraud's agar, gonococcus with this bacterium special culture medium) cultivates 48h in 73 DEG C,
Counting clump count also calculates killing rate.Result sees table 2.
Organic substance influence test is to drip, with the bacteria suspension containing variable concentrations calf serum, sheet of dying cloth, and makes bacterium sheet after drying,
Test by carrier quantitative method.
1.3 stability observing
The bottling of plant washing liquid stock solution is added a cover, places 14d in putting 54 DEG C of incubators, before and after placing, take testing sample respectively,
It is diluted to variable concentrations, measures it to escherichia coli killing effect.
1.4 metal protection tests
Select diameter 24.0mm, thick 0.2mm, circular rustless steel, carbon steel, copper and the aluminium flake of central aperture diameter 0.20mm.Examination
When testing, every kind of sheet metal takes three, is put in by same sheet metal in a container, and sterilized liquid stock solution is soaked 24h and changed liquid one
Secondary, continuous dipping 72h, wash erosion, dry.In soaking the forward and backward metal quality (being accurate to 0.1mg) that claims respectively, calculate
Corrosion rate (R).
1.5 toxicity test
1.5.1 kind adult mice regular grade in acute oral toxicity experimental selection Kunming is (in Gansu Chinese of Traditional Chinese Medicine's laboratory animal
The heart), body weight 18-22g, male and female half and half.Divide 4 groups and often organize 10.Design point the washing liquid stock solution of the present invention, 1:1 times of diluent, 1:2
Times 3 concentration groups of diluent and matched group.Fasting 12h, presses the disposable gastric infusion of 0.2ml/10g (body weight) to each group of mice,
Continuous Observation 14d, calculates LD50。
1.5.2 the disposable irritant test of skin takes healthy white rabbit (Gansu Chinese of Traditional Chinese Medicine's Experimental Animal Center), body weight
.25-3.5kg 4, male and female half and half.Before test, family's rabbit back spinal column diamond wool is respectively cut off about 3cm × 3cm by 24h, takes plant and washes
Liquid 0.02ml uniformly drips on 2 layers of 2.5cm × 2.5cm gauze, applies ointment or plaster on the skin of side, then covers with one layer of cellophane,
Use immobilization with adhesive tape again；Another side skin is coated with normal saline and compares；Effect 4h, with warm water remove residual test medicine, in l, 24,
48h observes the dermoreaction of site of action.
1.5.3 (Gansu Chinese of Traditional Chinese Medicine is real by regular grade adult healthy Female rabbits for vaginal mucomembranous irritant test laboratory animal
Center of testing provides), body weight .25-3.5kg, test point 4 groups, often group 4.Vagina administration, by disinfectant solution stock solution, 1:1 times dilute
Liquid, 3 concentration groups of 1:2 times of diluent and the normal saline (matched group) of sterilizing, each group all takes 0.2ml and drips and be respectively placed in cotton sliver
Take out after the intravaginal contact 4h of 4 treated animals, put to death animal after 24h, take out vagina tissue and observe.
2 results
The washing liquid nertralizer result of the test of table 1 present invention
Note: test temperature is 22 DEG C, the washing liquid stock solution of the present invention acts on 10min after adding bacterium sheet, adds nertralizer effect
10min, result is 3 test meansigma methodss.
Wherein, lotion 1 is the lotion that embodiment 1 prepares, and lotion 2 is the lotion that embodiment 2 prepares, lotion 3
The lotion prepared for embodiment 3, lotion 4 is the lotion that embodiment 4 prepares.Lower same.
The washing liquid of the table 2-1 present invention killing effect to microorganism
The washing liquid of the table 2-2 present invention killing effect to microorganism
2.1 nertralizer result of the tests
Result of the test shows, with the PBS containing 30g/L Tween 80+3g/L lecithin as nertralizer, neutralizes plant washing liquid
Stock solution, result hit the target (table 1).
2.2 bactericidal effect
Through inspection, plant washing liquid 1-4 all can effectively sterilize, and the highest with No. 2 sterilizing rates of plant washing liquid.Experiment below therefore
The performance continuing the washing liquid to the present invention as a example by plant washing liquid 2 is described.
No. 2 l:l times of diluents of plant washing liquid are to escherichia coli effect 5min on pieces of cloth, to staphylococcus aureus effect
15min, to Candida albicans effect 10min, reaches more than 80.00% (table 2-to Diplococcus gonorrhoeae effect 20min average bactericidal rate
1 and table 2-2).
2,3 organic substance influence test
When bacteria suspension is containing volume fraction 25% calf serum, the plant washing liquid diluted 1:1 times 2 (i.e. make by embodiment 2
The standby washing liquid obtained) kill escherichia coli and extended to more than 10min by 5min action time.Show in the presence of Organic substance this
Disinfectant solution bactericidal effect has a certain impact (table 3).
2.4 stability test
Testing result shows, after plant washing liquid 2 places 54 DEG C of incubator 30d, previous with placement to escherichia coli killing rate
Cause (table 4).
2.5 corrosivity
Test shows, soaks 72h, to aluminum, copper, carbon steel, the equal non-corrosiveness of rustless steel (table 5) through No. 2 stock solutions of plant washing liquid.
Table 3 Organic substance is on No. 2 impacts killing escherichia coli effect of plant washing liquid
Note: test temperature is 22 DEG C.Positive controls average bacterium number, escherichia coli are 2790000cuf/ sheet, golden yellow Portugal
Grape coccus is 129000Ocuf/ sheet, and Candida albicans is 1870000cuf/ sheet, Diplococcus gonorrhoeae 930000cuf/ sheet, and result is
3 test meansigma methodss.
Escherichia coli stability is killed test by table 4 plant washing liquid 2
Metal protection is tested by table 5 plant washing liquid 2
2.6 toxicity test
Experiment results proved, plant washing liquid 2 is equal to its mouse oral LD50 female, male > 5000mg/kg (body weight), true border
Nontoxic level.The disposable irritant test of skin and vaginal mucomembranous irritant test show, this disinfectant solution stock solution is to rabbit skin and vagina
Mucous membrane irritation reaction integration is 0, and skin belongs to non-stimulated without erythema, mucosa without edema.
3 discuss
The natural plant lotion of the present invention is combined with attapulgite with Chinese medicine extract, and its raw material sources are extensive, prepares work
Skill is simple.Result of the test shows, the plant washing liquid 1:l times diluent of the present invention, under the conditions of 22 DEG C, to escherichia coli on pieces of cloth
Effect 5min, to staphylococcus aureus effect 15min, to Candida albicans effect 10min, to Diplococcus gonorrhoeae effect
20min, killing rate all reaches more than 80.00%, has reached " disinfection technology standard " and has wanted carrier quantitative germicidal test sterilization is qualified
Ask.Organic substance has certain impact, this disinfectant solution stable performance to its bactericidal effect, and 54 DEG C of storage 30d, its bactericidal effect is without becoming
Change.Animal experiment proves, it belongs to nontoxic level, to mucocutaneous nonirritant.
Test example 2: clinical trial
Cow endometritis is a kind of reproductive system disease common in Cow product, is the weight causing barrenness of milk cow
Wanting one of reason, its sickness rate is higher, and typically more than 7%, indivedual cattle farms are up to 2.5%.Usually because of difficult labour, midwifery unreasonable
The caused pathogenic bacterial infection in puerperal such as mechanical injuries, placenta retention and specificity pathogen (such as brucella) attack and draw
Sending out, clinical treatment often results in the breeding difficultys such as cow oestrus cycle disorder, prolongation in oestrus, Repeat breeding not in time, and
Suffering from cattle milk yield to decline, serious sick cattle is forced to eliminate.According to mucosa injury degree and secretions character, card typically can be classified as
His property (slightly), stickiness (moderate) and purulence (severe) endometritis 3 type.The main cause of disease of this disease is antibacterial.Fortune
With antibiotic, (anti-microbial type chemotherapy endometritis is the method commonly used clinically, but this kind of method usually causes
Drug resistance, the generation of drug resistance, make dosage increase, increased frequency, and drug cost improves, because of antibiosis in fresh milk during treatment
The drug residues such as element, cause milk to go out of use, have a strong impact on the economic benefit of Cow product.For seeking more effectively to prevent and treat medicine
Thing, our seminar have developed and is convenient to medication, it is adaptable to the nursing of daily cow uteri, and prevention endometritis occurs,
And medicative plant lotion.In order to determine that cow endometritis is prevented and treated curative effect by this lotion, carry out following examination
Test.
1 materials and methods
1.1 material
1.1.1 trial drug: plant washing liquid 2 (washing liquid that i.e. embodiment 2 prepares) is blue by the Chinese Academy of Agricultural Sciences
State herding is developed with veterinary drug institute medicament chamber.
1.1.2 experimental animal: Kai Yue pasture, Gansu chooses 30, age 3-7 year, parity 2-5 tire, per rectum inspection, the moon
Road inspection and secretions character observation, be diagnosed as the Postpartum Cows suffering from endometritis.
1.2 method
1.2.1 endometritis diagnostic criteria: divide slightly by the state of an illness, moderate and severe 3 type.
Slightly (Catarrhal endometritis): vagina have mild inflammation, hyperemia, flushing, have the little block secretions of white or
Transparent mucus containing floccule.Vaginal part of cervix swelling is congested.Rectal palpation Uterus wall slightly thickens, ungauged regions reaction or
React inconspicuous.
Moderate (stickiness purulence endometritis): vagina has moderate inflammation, hyperemia, flushing, have a small amount of floccule or
Purulent secretion.Vaginal part of cervix hyperemia, flushing, swelling, there is purulent secretion.Rectal palpation: cornua uteri increases thick, Uterus wall
Uneven distribution, has slight fluctuation.
Severe (purulence endometritis): vagina has extensive inflammation, and congested, flushing, mucosa has damage, has more purulence
Secretions.Cervical orifice is opened a business ", there is purulent secretion.Rectal palpation, cornua uteri increases thick, and Uterus wall plumpness is uneven, in cavity of uterus
Accumulation liquid, fluctuation is obvious, rinses a large amount of yellowish-brown or the cotton-shaped purulent secretion of canescence seen from uterus.
1.2.2 be grouped and process: by milch cow by light, in, be heavily grouped.
1.2.3 administration regimen is tested: plant washing liquid 2 uses the dispensing of intrauterine infusion method.Slightly, moderate and severe institute
There is case, before administration, all carry out clearing heat in the pericardium process with normal saline, then suffer from cattle the most disposable intrauterine and inject for every
500ml medicinal liquid, carries out rectum kneading uterine simultaneously, makes medicine fully dispersed.
Medication 3 times/day after successive administration, 3rd are 1 course for the treatment of.After process, every day observes and suffers from cattle vaginal secretions discharge feelings
Condition, the change of examination per rectum uterine state.
1.2.4 curative effect determinate standard
Clinical indexes: vaginal speculum check item is vaginal discharge, vaginal mucosa swelling, hyperemia, hemorrhage, downright bad
Degree, uterine cervix swelling, hyperemia, extent of hemorrhage.
Examination per rectum: cornua uteri size, quality, hydrops situation.
General physical checkup: body temperature and appetite, the mental status.Observe dispensing during and dispensing after with or without local irritation reaction and
Topically or systemically anaphylaxis, and record time started, persistent period, degree, process process and lapse to.
Efficacy determination: by checking vagina, the change of uterine state and secretions quantity, color and luster, character after medication, to sentence
Disconnected curative effect.
Effective: after medication, inflammation of uterus disappears, cornua uteri or body of uterus palpation, without swollen hard, suffer from cattle without pain reaction, uterus
After shrinking or exerting duty, secretions without exception is discharged or discharges the most normal.
Effective: after medication, inflammation of uterus is substantially alleviated, palpation pain and swelling substantially alleviate, secretions quantity and character
Close to normal.
Invalid: after medication, inflammation of uterus has no and turns slow, and uterus palpation swelling and pain are still it is obvious that uterine secretion thing has no
Take a turn for the better and even deteriorate.
3 results and analysis
The case that the safety institute useful plant washing liquid 2 of milch cow is treated by 3.1 trial drugs, after being administered 1 course for the treatment of,
All do not observe whole body or local anaphylaxis and local irritation reaction.
3.2 different batches plant washing liquids 2 are to the clinical effectiveness 3 batch plant washing liquid 2 of endometritis to milch cow not
It is shown in Table 6 with the therapeutic effect of degree endometritis.As can be seen from Table 6,3 batch plant washing liquid 2 treats same degree milk
Cattle endometritis, curative effect is the most close.
3.3 plant washing liquids 2 are to the clinical effectiveness plant washing liquid 2 of endometritis in various degree to milch cow in various degree
The therapeutic effect of endometritis is shown in Table 1.As can be seen from Table 1, plant washing liquid 2,1 course for the treatment of, (3/, 3rd be a treatment
Journey).After 1 course for the treatment of is administered, person evident in efficacy totally 26, total obvious effective rate reaches, and 86.6%.Plant washing liquid 2 to slight, moderate,
The effective percentage of severe 3 class difference state of an illness group is respectively 100%, 90% and 70%.
4 discuss and conclusion
The factor causing cow endometritis is a lot, but the invasion and attack of pathogen infection is to cause endometritis to occur
Immediate cause, owing to this disease often betides puerperal in early days (1-10d), decline with the resistance of body in puerperal also have close
Relation, therefore, its Therapeutic Principle is to improve Abwehrkraft des Koepers, eliminates and infects, and removes uterus exudate.
Carrying out uterine perfusion with antibiotic or anti-microbial type chemicals is the predominant methods treating endometritis, but by
In life-time service, medication the most lack of standardization, cause Drug resistance, drug resistance seriously to produce, not only affect curative effect of medication, and make
Obtaining dosage to increase, increased frequency, drug cost improves, and antibiotic, the drug residue of anti-microbial type chemicals, make cattle simultaneously
Milk is discarded during treating, and makes the application of the anti-microbial type conventional medicines such as antibiotic be restricted.Herba Houttuyniae belongs to antipyretic and antidotal type sky
So plant amedica, have heat clearing away, detoxify, subside a swelling, go blood stasis, eliminating carbuncle, assault fortified position, the effect such as evacuation of pus.Modern pharmacology research shows, its
The various bacteria such as staphylococcus aureus, Hemolytic streptococcus, enterobacteria, fungus there is bacteriostasis.
Table 6 same dose and the curative effect of administration number of times
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to aforementioned reality
Executing example to be described in detail the present invention, for a person skilled in the art, it still can be to aforementioned each enforcement
Technical scheme described in example is modified, or wherein portion of techniques feature is carried out equivalent.All essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (9)
1. a plant type milch cow special uterus washing liquid, it is characterised in that: utilize the attapulgite of activation that plant extract is carried out
Absorption, is made into lotion,
Wherein, described plant extract is mainly composed of Herba Sophorae alopecuroidis alkali, Flos Lonicerae extract, Herba Hyperici perforati extract, Huang
A kind of reed mentioned in ancient books extract, Herba Leonuri extract Flos Carthami extract.
The special uterus of plant type milch cow the most according to claim 1 washing liquid, it is characterised in that: each component by weight:
Herba Sophorae alopecuroidis alkali 5-40 part, Flos Lonicerae extract 10-25 part, Herba Hyperici perforati extract 5-20 part, Radix Scutellariae extract 10-25 part, benefit
Herba Linderniae Crustaceae extract 20-35 part, Flos Carthami extract 10-25 part, attapulgite 2-5 part；As preferably, the mass parts of Herba Sophorae alopecuroidis alkali is
5-20 part.
The special uterus of plant type milch cow the most according to claim 2 washing liquid, it is characterised in that: each component by weight:
15 parts of Herba Sophorae alopecuroidis alkali, Flos Lonicerae extract 15 parts, Herba Hyperici perforati extract 10 parts, Radix Scutellariae extract 15 parts, Herba Leonuri extract
25 parts, Flos Carthami extract 15 parts, attapulgite 3 parts.
4. according to the plant type milch cow special uterus washing liquid described in any one of claim 1-3, it is characterised in that: described Herba Sophorae alopecuroidis
In alkali, total alkali is 30%；Chlorogenic acid content in described Flos Lonicerae extract is 20%；In described Herba Hyperici perforati extract
Determination of Hypericin from Extraction is 0.3%.
5. according to the preparation method of the plant type milch cow special uterus washing liquid described in any of the above claim, it is characterised in that:
The preparation method of 1000ml washing liquid is:
1) weighing attapulgite 2-5g by proportioning, add 100-250ml sour water and boil 30 minutes, making pH is 3, adjusts after placing room temperature
Joint pH is 7, boils to remove wherein solubility and volatile impurity in pure water, filters precipitation, at 100 DEG C of oven drying 2h,
Activate 2h at 500 DEG C again, obtain active attapulgite；
2) plant extract taking proportional quantity adds pure water dilution, makes mix homogeneously；
3) add the active attapulgite that step 1) prepares, after stirring at normal temperature 30min, add tween 80,1,3-the third two
Alcohol；
4) pure water is added to 1000ml；
5) stirring at normal temperature 30min；
6) subpackage, high temperature sterilize, obtain washing liquid.
The preparation method of plant type milch cow special uterus washing liquid the most according to claim 5, it is characterised in that: described step
Rapid 1) regulating pH with the sodium hydroxide solution of 5% after placing room temperature in is 7；Pure water boils 1h.
The preparation method of plant type milch cow special uterus washing liquid the most according to claim 5, it is characterised in that: described step
Rapid 3) amount adding tween 80 in is the 10% of washing liquid weight, amount is washing liquid weight the 5% of 1,3-propylene glycol.
8. the plant type milch cow special uterus washing liquid described in any one of claim 1-4 is at preparation treatment cow endometritis
Application in medicine.
9. a medicine, it is characterised in that: the effective ingredient of described medicine is the plant type milk described in any one of claim 1-4
Cattle special uterus washing liquid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106668139A (en) * | 2016-12-13 | 2017-05-17 | 中国农业科学院兰州畜牧与兽药研究所 | Externally used medicament for hoof of milk cow as well as preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102743503A (en) * | 2012-07-13 | 2012-10-24 | 宁夏大学 | Method for preparing Chinese medicinal compound effervescent tablets for treating endometritis of cows |
| CN103239554A (en) * | 2013-05-10 | 2013-08-14 | 贵州省畜牧兽医研究所 | Chinese medicine composition for treating endometritis of dairy cow and preparation method thereof |
| CN104127534A (en) * | 2014-08-26 | 2014-11-05 | 郭庆 | Preparation method of traditional Chinese medicine microecological preparation for preventing endometritis of dairy cattle |
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2016
- 2016-08-08 CN CN201610642885.4A patent/CN106138206A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102743503A (en) * | 2012-07-13 | 2012-10-24 | 宁夏大学 | Method for preparing Chinese medicinal compound effervescent tablets for treating endometritis of cows |
| CN103239554A (en) * | 2013-05-10 | 2013-08-14 | 贵州省畜牧兽医研究所 | Chinese medicine composition for treating endometritis of dairy cow and preparation method thereof |
| CN104127534A (en) * | 2014-08-26 | 2014-11-05 | 郭庆 | Preparation method of traditional Chinese medicine microecological preparation for preventing endometritis of dairy cattle |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106668139A (en) * | 2016-12-13 | 2017-05-17 | 中国农业科学院兰州畜牧与兽药研究所 | Externally used medicament for hoof of milk cow as well as preparation method and application thereof |
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