CN106135696B - Application of the CS-ZnO as intestine of young pigs mucosal immunity reinforcing agent - Google Patents
Application of the CS-ZnO as intestine of young pigs mucosal immunity reinforcing agent Download PDFInfo
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- CN106135696B CN106135696B CN201610519008.8A CN201610519008A CN106135696B CN 106135696 B CN106135696 B CN 106135696B CN 201610519008 A CN201610519008 A CN 201610519008A CN 106135696 B CN106135696 B CN 106135696B
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- 239000012744 reinforcing agent Substances 0.000 title claims abstract description 8
- 241000282887 Suidae Species 0.000 title claims description 12
- 210000000936 intestine Anatomy 0.000 title claims description 4
- 230000036039 immunity Effects 0.000 title description 8
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 claims abstract description 80
- 206010012735 Diarrhoea Diseases 0.000 claims abstract description 37
- 229920001661 Chitosan Polymers 0.000 claims abstract description 32
- 239000011787 zinc oxide Substances 0.000 claims abstract description 26
- 239000000654 additive Substances 0.000 claims abstract description 12
- 230000000996 additive effect Effects 0.000 claims abstract description 12
- 239000011701 zinc Substances 0.000 claims abstract description 12
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 11
- 241001465754 Metazoa Species 0.000 claims abstract description 10
- RNWHGQJWIACOKP-UHFFFAOYSA-N zinc;oxygen(2-) Chemical class [O-2].[Zn+2] RNWHGQJWIACOKP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 230000000968 intestinal effect Effects 0.000 claims abstract description 9
- 230000003053 immunization Effects 0.000 claims abstract description 5
- 238000002649 immunization Methods 0.000 claims abstract description 5
- 244000144972 livestock Species 0.000 claims description 2
- 244000144977 poultry Species 0.000 claims description 2
- 239000008267 milk Substances 0.000 claims 1
- 210000004080 milk Anatomy 0.000 claims 1
- 235000013336 milk Nutrition 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 6
- 230000008944 intestinal immunity Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 36
- 210000004027 cell Anatomy 0.000 description 23
- 230000002354 daily effect Effects 0.000 description 18
- 102000007296 Mucin-2 Human genes 0.000 description 14
- 108010008705 Mucin-2 Proteins 0.000 description 14
- 235000014590 basal diet Nutrition 0.000 description 12
- 108010002350 Interleukin-2 Proteins 0.000 description 11
- 230000012010 growth Effects 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- 210000004347 intestinal mucosa Anatomy 0.000 description 8
- 238000004659 sterilization and disinfection Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 210000004400 mucous membrane Anatomy 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 6
- 230000036737 immune function Effects 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 238000008157 ELISA kit Methods 0.000 description 4
- 210000001015 abdomen Anatomy 0.000 description 4
- 239000006027 corn-soybean meal Substances 0.000 description 4
- 230000034994 death Effects 0.000 description 4
- 235000005911 diet Nutrition 0.000 description 4
- 230000037213 diet Effects 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 4
- 239000005457 ice water Substances 0.000 description 4
- 210000003405 ileum Anatomy 0.000 description 4
- 229940099472 immunoglobulin a Drugs 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 239000002504 physiological saline solution Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 230000003248 secreting effect Effects 0.000 description 4
- 238000003307 slaughter Methods 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 244000052769 pathogen Species 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
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- 102000004190 Enzymes Human genes 0.000 description 2
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- 230000004913 activation Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- -1 Glycan zinc oxide composites Chemical class 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
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- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
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- 229920002678 cellulose Polymers 0.000 description 1
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- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000006196 deacetylation Effects 0.000 description 1
- 238000003381 deacetylation reaction Methods 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
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- 230000028996 humoral immune response Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/30—Zinc; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/716—Glucans
- A61K31/722—Chitin, chitosan
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Molecular Biology (AREA)
- Inorganic Chemistry (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
A kind of application the invention discloses chitosan zinc oxide as weanling pig Intestinal Mucosal Immunization reinforcing agent can be used to improve weanling pig intestine immunity ability, reduce diarrhea of weaned piglets rate, reduce diarrhea of weaned piglets rate by 8.7%-63.8%.Usage are as follows: chitosan zinc oxide composites directly make an addition in animal and fowl fodder, mix rear feeding animals thoroughly, and the mass percent that the chitosan zinc oxide composites account for animal and fowl fodder after addition is 0.01%-0.06%；The above additive amount is in terms of zinc.
Description
Technical field
It is specifically a kind of to be related to CS-ZnO compound conduct the present invention relates to a kind of application of compound in animal and fowl fodder
Application in weanling pig Intestinal Mucosal Immunization reinforcing agent.
Background technique
CS-ZnO (chitosan zinc oxide) compound be it is a kind of chitosan and zinc oxide are dissolved in acid, be spray dried to small
The compound of grain shape.The compound not only has biocompatibility, but also has very strong antibiotic property, is that one kind has practical valence very much
The sanitary antimicrobial material of value, has broad application prospects, but not yet appears in the newspapers as weanling pig Intestinal Mucosal Immunization reinforcing agent
Road.
Chitosan is the product of chitin deacetylation, and chitin is the polymer that cellulose is only second on the earth.Shell is poly-
Sugar has nontoxic, a good biological compatibility, biological degradability and the features such as can be absorbed, and is widely used in medicine, chemical industry,
Agricultural etc..Cholesterol is reduced since chitosan has, strengthen immunity, the effect of restraining and sterilizing bacteria also can be micro- by adjusting enteron aisle
The growth of biota promotion livestock and poultry.As antibacterial agent, chitosan and its derivative has preferable antibacterial activity, can inhibit
The growth and breeding of fungi, bacterium and virus.Thought that its possible mechanism has three by the end of 2013: first is that more due to chitosan
Polycation is easy to the negatively charged group effect of fungal cell surface, to change the mobility of pathogen cell membrane and lead to
Permeability；Second is that the duplication and transcription of interference DNA；Third is that blocking pathogen metabolism.In addition, chitosan have activation airframe systems,
The series of biologic effect for mediating airframe systems, improves the system function of phagocyte.That there is bacteriums is more for Macrophage Surface
The receptor of sugar, and analog of the chitosan as bacterial polysaccharides, energy stimulating expression of macrophage activation, generate following reaction: promoting it
Phagocytic function enhances its synergistic effect in other immune responses, to realize body to T cell, NK cell and B cell
It adjusts, mediates the cellullar immunologic response and humoral immune response of body.Therefore, chitosan also has the work of the immunological regulation to body
With.
Zinc participates in the synthesis of hundreds of enzyme in vivo, is the constituent of some important enzymes, immune with body, anti-oxidant,
It grows closely related.Zinc oxide improves growth performance, is that one kind is widely utilized that feed adds in the upper energy prophylactic treatment diarrhea of production
Add agent, additive amount has reached 3 000mg/kg in the daily ration of piglet.It is super that Zn content in excrement largely is caused using zinc oxide
Mark, causes pollution to environment.
Diarrhea of weaned piglets is always global problem, although the high dose zinc oxide of 3 000mg/kg can be controlled preferably
Diarrhea of weaned piglets rate processed, but the absorptivity of inorganic zinc and utilization rate are lower, 90% or more zinc is excreted with excrement, is made
At the wasting of resources and environmental pollution.Therefore, weanling pig immune function how is improved, diarrhea of weaned piglets is reduced, is that live pig is supported
Grow the critical issue of industry sustainable development.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of chitosan zinc oxide as weanling pig Intestinal Mucosal Immunization
The application of reinforcing agent.
In order to solve the above technical problem, the present invention provides a kind of chitosan zinc oxide composites as weanling pig enteron aisle
The application of mucosal immunity reinforcing agent reduces diarrhea of weaned piglets rate for improving weanling pig intestine immunity ability.
The chitosan zinc oxide composites directly make an addition in animal and fowl fodder, mix rear feeding animals, the shell thoroughly
Glycan zinc oxide composites account for addition after animal and fowl fodder mass percent be 0.01%-0.06%；The above additive amount is in terms of zinc
(that is, being 100~600mg/kg).
Diarrhea of weaned piglets rate reduces 8.7%-63.8%.
In the present invention, chitosan zinc oxide composites are the changes according to " chitosan/ZnO composite material preparation and characterization "
It learns solution-deposition method to be prepared, in the compound, the weight ratio of chitosan and zinc oxide is 1:2.
The invention has the following beneficial effects:
After entering in animal body due to chitosan zinc oxide composites, pathogen can be done directly on, kill or inhibit to have
The growth of evil bacterium, improves intestine of young pigs environment, can make the reduction of proinflammatory factor content, presses down scorching factor content and improves, to alleviate inflammation
Disease reaction, improves mucosal immune function.Therefore, chitosan zinc oxide composites are as a kind of safe and efficient immune increasing
Strong agent can reduce diarrhea of weaned piglets rate as feed addictive, improve weanling pig daily gain, promote piglet healthy growth,
It will have broad application prospects.
Specific embodiment
A specific embodiment of the invention can carry out any selection in each numberical range described in foregoing invention content
Replacement, and constitute different embodiments, it is not limited to following embodiment.
Embodiment 1:
The Ternary Pig three way cross piglet 90 that this test selects 21 ages in days that weight is 5.5kg to wean, is randomly divided into 3 groups
(every group of 3 repetitions, each repetition 10), is randomly divided into 3 groups by feeding experiment requirement.3 kinds of different daily rations, control are fed in examination respectively
Group is corn-soybean meal basal diet, and it is multiple that test group adds 100mg/kg chitosan zinc oxide respectively on the basis of basal diet
Object (CS-ZnO) and 100mg/kg chitosan and zinc oxide mix (mass ratio of CS+ZnO, CS and ZnO are 1:2) are closed, it is above
In terms of zinc, basal diet needs to prepare additive amount referring to U.S. NRC (1998) piglet nutrition.Test pig group as unit of column
It raises, be freely eaten and drink water.It is carried out disinfection with 1% disinfection prestige to pig house before test, preliminary trial period 7 days, the positive examination phase 28 days.Experimental period
Between every day entry feed consumption, count diarrhea and death condition, after the test, fasting 12h weighs after free water, point
6 (every repetitions 2) are not randomly selected from each processing group, totally 18 test pigs carry out slaughter experiment, take partial ileum mucous membrane, add
Enter ice-cold physiological saline, be sufficiently homogenized in ice water, be made 10% tissue homogenate supernatant be sub-packed in Eppendorf pipe ,-
80 DEG C save for use.Secretory immunoglobulin A (SIgA), mucin 2 (MUC2), cell factor IL-2 and cell factor IL-
The measurement of 10 contents is all made of ELISA kit.Daily gain, feed-weight ratio and diarrhea rate are calculated, wherein diarrhea rate (%)=total abdomen
Rush down a number/(piglet head number × test number of days) × 100%.Test data is as shown in Table 1 and Table 2.
Test result shows that zincification group average daily gain is all remarkably higher than control group, wherein CS-ZnO group average daily gain
3.7% is improved than CS+ZnO group；Zincification group feed-weight ratio is substantially less than control group, wherein CS-ZnO group feed-weight ratio ratio CS+ZnO
Group reduces by 6.1%；Zincification group diarrhea rate is substantially less than control group, and wherein CS-ZnO group diarrhea rate ratio CS+ZnO group reduces
16.9%；Zincification group intestinal mucosa SIgA content is all remarkably higher than control group, and wherein CS-ZnO group ratio CS+ZnO group improves
6.4%；Zincification group intestinal mucosa MUC2 content is all remarkably higher than control group, and wherein CS-ZnO group ratio CS+ZnO group improves
11.0%；Zincification group mucomembranous cell factor IL-2 content is substantially less than control group, and CS-ZnO group mucomembranous cell factor IL-2 contains
Amount reduces 12.9% than CS+ZnO group；Zincification group mucomembranous cell factor IL-10 content is all remarkably higher than control group, CS-ZnO group
Mucomembranous cell factor IL-10 content ratio CS+ZnO group improves 8.4%.As a result it prompts, 100mg/kg CS-ZnO is added in diet
Weanling pig mucosal immune function can be significantly improved, reduces diarrhea rate, and then improve its growth performance.
Influence of 1 CS-ZnO of table to Production Performance of Weaning Pigs
Influence of 2 CS-ZnO of table to weanling pig Intestinal Mucosal Immunity function
Embodiment 2:
The Ternary Pig three way cross piglet 90 that this test selects 24 ages in days that weight is 6.0kg to wean, is randomly divided into 3 groups
(every group of 3 repetitions, each repetition 10), is randomly divided into 3 groups by feeding experiment requirement.3 kinds of different daily rations, control are fed in examination respectively
Group is corn-soybean meal basal diet, and it is multiple that test group adds 250mg/kg chitosan zinc oxide respectively on the basis of basal diet
Object (CS-ZnO) and 250mg/kg chitosan and zinc oxide mix (mass ratio of CS+ZnO, CS and ZnO are 1:2) are closed, it is above
In terms of zinc, basal diet needs to prepare additive amount referring to U.S. NRC (1998) piglet nutrition.Test pig group as unit of column
It raises, be freely eaten and drink water.It is carried out disinfection with 1% disinfection prestige to pig house before test, preliminary trial period 7 days, the positive examination phase 28 days.Experimental period
Between every day entry feed consumption, count diarrhea and death condition, after the test, fasting 12h weighs after free water, point
6 (every repetitions 2) are not randomly selected from each processing group, totally 18 test pigs carry out slaughter experiment, take partial ileum mucous membrane, add
Enter ice-cold physiological saline, be sufficiently homogenized in ice water, be made 10% tissue homogenate supernatant be sub-packed in Eppendorf pipe ,-
80 DEG C save for use.Secretory immunoglobulin A (SIgA), mucin 2 (MUC2), cell factor IL-2 and cell factor IL-
The measurement of 10 contents is all made of ELISA kit.Daily gain, feed-weight ratio and diarrhea rate are calculated, wherein diarrhea rate (%)=total abdomen
Rush down a number/(piglet head number × test number of days) × 100%.Test data is as shown in Table 3 and Table 4.
Test result shows that zincification group average daily gain is all remarkably higher than control group, wherein CS-ZnO group average daily gain
4.8% is improved than CS+ZnO group；Zincification group feed-weight ratio is substantially less than control group, wherein CS-ZnO group feed-weight ratio ratio CS+ZnO
Group reduces by 7.8%；Zincification group diarrhea rate is substantially less than control group, and wherein CS-ZnO group diarrhea rate ratio CS+ZnO group reduces
17.0%；Zincification group intestinal mucosa SIgA content is all remarkably higher than control group, wherein CS-ZnO group mucous membrane SIgA content ratio CS+ZnO
Group improves 8.3%；Zincification group intestinal mucosa MUC2 content is all remarkably higher than control group, wherein CS-ZnO group mucous membrane SIgA content ratio
CS+ZnO group improves 15.5%；Zincification group mucomembranous cell factor IL-2 content is substantially less than control group, CS-ZnO group ratio CS+
ZnO group reduces 17.8%；Zincification group mucomembranous cell factor IL-10 content is all remarkably higher than control group, and CS-ZnO group mucous membrane is thin
Intracellular cytokine IL-10 content ratio CS+ZnO group improves 8.9%.As a result it prompts, 250mg/kg CS-ZnO is added in diet can be obvious
Weanling pig mucosal immune function is improved, reduces diarrhea rate, and then improve its growth performance.
Influence of 3 CS-ZnO of table to Production Performance of Weaning Pigs
Influence of 4 CS-ZnO of table to weanling pig Intestinal Mucosal Immunity function
Embodiment 3:
The Ternary Pig three way cross piglet 90 that this test selects 28 ages in days that weight is 6.5kg to wean, is randomly divided into 3 groups
(every group of 3 repetitions, each repetition 10), is randomly divided into 3 groups by feeding experiment requirement.3 kinds of different daily rations, control are fed in examination respectively
Group is corn-soybean meal basal diet, and it is multiple that test group adds 600mg/kg chitosan zinc oxide respectively on the basis of basal diet
Object (CS-ZnO) and 600mg/kg chitosan and zinc oxide mix (mass ratio of CS+ZnO, CS and ZnO are 1:2) are closed, it is above
In terms of zinc, basal diet needs to prepare additive amount referring to U.S. NRC (1998) piglet nutrition.Test pig group as unit of column
It raises, be freely eaten and drink water.It is carried out disinfection with 1% disinfection prestige to pig house before test, preliminary trial period 7 days, the positive examination phase 28 days.Experimental period
Between every day entry feed consumption, count diarrhea and death condition, after the test, fasting 12h weighs after free water, point
6 (every repetitions 2) are not randomly selected from each processing group, totally 18 test pigs carry out slaughter experiment, take partial ileum mucous membrane, add
Enter ice-cold physiological saline, be sufficiently homogenized in ice water, be made 10% tissue homogenate supernatant be sub-packed in Eppendorf pipe ,-
80 DEG C save for use.Secretory immunoglobulin A (SIgA), mucin 2 (MUC2), cell factor IL-2 and cell factor IL-
The measurement of 10 contents is all made of ELISA kit.Daily gain, feed-weight ratio and diarrhea rate are calculated, wherein diarrhea rate (%)=total abdomen
Rush down a number/(piglet head number × test number of days) × 100%.Test data is as shown in table 5 and table 6.
Test result shows that zincification group average daily gain is all remarkably higher than control group, wherein CS-ZnO group average daily gain
5.4% is improved than CS+ZnO group；Zincification group feed-weight ratio is substantially less than control group, wherein CS-ZnO group feed-weight ratio ratio CS+ZnO
Group reduces by 5.0%；Zincification group diarrhea rate is substantially less than control group, and wherein CS-ZnO group diarrhea rate ratio CS+ZnO group reduces
20.8%；Zincification group intestinal mucosa SIgA content is all remarkably higher than control group, and wherein CS-ZnO group SIgA content is mentioned compared with CS+ZnO group
It is high by 10.3%；Zincification group intestinal mucosa MUC2 content is all remarkably higher than control group, and wherein CS-ZnO group MUC2 content is compared with CS+ZnO
Group improves 25.0%；Zincification group mucomembranous cell factor IL-2 content be substantially less than control group, CS-ZnO group mucomembranous cell because
Sub- IL-2 content ratio CS+ZnO group reduces 20.5%；Zincification group mucomembranous cell factor IL-10 content is all remarkably higher than control group,
CS-ZnO group mucomembranous cell factor IL-10 content ratio CS+ZnO group improves 9.0%.As a result it prompts, 600mg/ is added in diet
Kg CS-ZnO can significantly improve weanling pig mucosal immune function, reduce diarrhea rate, and then improve its growth performance.
Influence of 5 CS-ZnO of table to Production Performance of Weaning Pigs
Influence of 6 CS-ZnO of table to weanling pig Intestinal Mucosal Immunity function
Embodiment 4:
The Ternary Pig three way cross piglet 90 that this test selects 22 ages in days that weight is 5.6kg to wean, is randomly divided into 3 groups
(every group of 3 repetitions, each repetition 10), is randomly divided into 3 groups by feeding experiment requirement.3 kinds of different daily rations, control are fed in examination respectively
Group is corn-soybean meal basal diet, and it is multiple that test group adds 400mg/kg chitosan zinc oxide respectively on the basis of basal diet
Object (CS-ZnO) and 400mg/kg chitosan and zinc oxide mix (mass ratio of CS+ZnO, CS and ZnO are 1:2) are closed, it is above
In terms of zinc, basal diet needs to prepare additive amount referring to U.S. NRC (1998) piglet nutrition.Test pig group as unit of column
It raises, be freely eaten and drink water.It is carried out disinfection with 1% disinfection prestige to pig house before test, preliminary trial period 7 days, the positive examination phase 28 days.Experimental period
Between every day entry feed consumption, count diarrhea and death condition, after the test, fasting 12h weighs after free water, point
6 (every repetitions 2) are not randomly selected from each processing group, totally 18 test pigs carry out slaughter experiment, take partial ileum mucous membrane, add
Enter ice-cold physiological saline, be sufficiently homogenized in ice water, be made 10% tissue homogenate supernatant be sub-packed in Eppendorf pipe ,-
80 DEG C save for use.Secretory immunoglobulin A (SIgA), mucin 2 (MUC2), cell factor IL-2 and cell factor IL-
The measurement of 10 contents is all made of ELISA kit.Daily gain, feed-weight ratio and diarrhea rate are calculated, wherein diarrhea rate (%)=total abdomen
Rush down a number/(piglet head number × test number of days) × 100%.Test data is as shown in table 7 and table 8.
Test result shows that zincification group average daily gain is all remarkably higher than control group, wherein CS-ZnO group average daily gain
4.3% is improved than CS+ZnO group；Zincification group feed-weight ratio is substantially less than control group, wherein CS-ZnO group feed-weight ratio ratio CS+ZnO
Group reduces by 8.7%；Zincification group diarrhea rate is substantially less than control group, and wherein CS-ZnO group diarrhea rate ratio CS+ZnO group reduces
12.9%；Zincification group intestinal mucosa SIgA content is all remarkably higher than control group, and wherein CS-ZnO group SIgA content is mentioned compared with CS+ZnO group
It is high by 9.9%；Zincification group intestinal mucosa MUC2 content is all remarkably higher than control group, and wherein CS-ZnO group MUC2 content is compared with CS+ZnO group
Improve 6.3%；Zincification group mucomembranous cell factor IL-2 content is substantially less than control group, the CS-ZnO group mucomembranous cell factor
IL-2 content ratio CS+ZnO group reduces 19.2%；Zincification group mucomembranous cell factor IL-10 content is all remarkably higher than control group,
CS-ZnO group mucomembranous cell factor IL-10 content ratio CS+ZnO group improves 8.3%.As a result it prompts, 400mg/ is added in diet
Kg CS-ZnO can significantly improve weanling pig mucosal immune function, reduce diarrhea rate, and then improve its growth performance.
Influence of 7 CS-ZnO of table to Production Performance of Weaning Pigs
Influence of 8 CS-ZnO of table to weanling pig Intestinal Mucosal Immunity function
Comparative example 1-1, the additive amount of the chitosan zinc oxide composites (CS-ZnO) in embodiment 1 is divided by 100mg/kg
It is not changed to 50mg/kg and 800mg/kg, for the above additive amount in terms of zinc, remaining is equal to embodiment 1.
When additive amount is 50mg/kg, grice diarrhoea rate (%) are as follows: 13.8 ± 1.31.
When additive amount is 800mg/kg, grice diarrhoea rate (%) are as follows: 10.0 ± 0.85.
The above list is only a few specific embodiments of the present invention for finally, it should also be noted that.Obviously, this hair
Bright to be not limited to above embodiments, acceptable there are many deformations.Those skilled in the art can be from present disclosure
All deformations for directly exporting or associating, are considered as protection scope of the present invention.
Claims (2)
1. application of the chitosan zinc oxide as weanling pig Intestinal Mucosal Immunization reinforcing agent, it is characterised in that: disconnected for improving
Milk intestine of young pigs immunocompetence reduces diarrhea of weaned piglets rate；The chitosan zinc oxide composites directly make an addition to livestock and poultry
In feed, rear feeding animals are mixed thoroughly, the mass percent that the chitosan zinc oxide composites account for animal and fowl fodder after addition is
0.01%-0.06%；The above additive amount is in terms of zinc.
2. application according to claim 1, it is characterised in that: reduce diarrhea of weaned piglets rate by 8.7%-63.8%.
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| CN102335196A (en) * | 2011-10-25 | 2012-02-01 | 浙江大学 | Use of supported nano zinc oxide as anti-diarrhea agent for weaned pigs and using method |
| CN102370076A (en) * | 2010-08-08 | 2012-03-14 | 嘉善百世威生物技术有限公司 | Mixed feed for piglings |
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| CN103005212A (en) * | 2012-12-26 | 2013-04-03 | 浙江大学 | Feed additive for improving intestinal functions of piglets and application of feed additive |
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| CN102370076A (en) * | 2010-08-08 | 2012-03-14 | 嘉善百世威生物技术有限公司 | Mixed feed for piglings |
| CN102335196A (en) * | 2011-10-25 | 2012-02-01 | 浙江大学 | Use of supported nano zinc oxide as anti-diarrhea agent for weaned pigs and using method |
| CN102771683A (en) * | 2012-08-30 | 2012-11-14 | 安佑（中国）动物营养研发有限公司 | Bacillus-subtilis-contained artificial milk extract premix for pigs and application of premix |
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