CN106119189B - 胎儿有核红细胞的分离纯化方法及试剂盒 - Google Patents

胎儿有核红细胞的分离纯化方法及试剂盒 Download PDF

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CN106119189B
CN106119189B CN201610543705.7A CN201610543705A CN106119189B CN 106119189 B CN106119189 B CN 106119189B CN 201610543705 A CN201610543705 A CN 201610543705A CN 106119189 B CN106119189 B CN 106119189B
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cells
blood sample
antibody
separation
red blood
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CN106119189A (zh
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夏庆杰
杨林
杨雪飞
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Shandong Yada Pharmacy Co ltd
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Shandong Yada Pharmacy Co ltd
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Priority to PCT/CN2017/092462 priority patent/WO2018010632A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0641—Erythrocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201610543705.7A 2016-07-11 2016-07-11 胎儿有核红细胞的分离纯化方法及试剂盒 Active CN106119189B (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN202010186566.3A CN111500534B (zh) 2016-07-11 2016-07-11 分离纯化胎儿有核红细胞的试剂盒
CN201610543705.7A CN106119189B (zh) 2016-07-11 2016-07-11 胎儿有核红细胞的分离纯化方法及试剂盒
PCT/CN2017/092462 WO2018010632A1 (fr) 2016-07-11 2017-07-11 Procédé de séparation et de purification de globules rouges nucléés fœtaux, et kit de réactifs

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CN201610543705.7A CN106119189B (zh) 2016-07-11 2016-07-11 胎儿有核红细胞的分离纯化方法及试剂盒

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Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111500534B (zh) * 2016-07-11 2022-11-29 山东亚大药业有限公司 分离纯化胎儿有核红细胞的试剂盒
CN106754691A (zh) * 2016-12-12 2017-05-31 扬州大学 一种从家禽全血样本快速分离高纯度红细胞的方法
JP6234542B1 (ja) * 2016-12-27 2017-11-22 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ 胎児細胞由来染色体ｄｎａの取得方法
CN110643564A (zh) * 2018-06-26 2020-01-03 深圳市北科生物科技有限公司 基于磁式分离的全自动化细胞分离方法及装置
CN108956573A (zh) * 2018-08-02 2018-12-07 重庆医科大学附属儿童医院 一种检测xiap蛋白的试剂盒及其应用
CN114480373A (zh) * 2022-04-01 2022-05-13 北京贝瑞和康生物技术有限公司 一种富集孕妇血浆中胎儿dna的方法和试剂盒

Citations (2)

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WO2004029221A2 (fr) * 2002-09-27 2004-04-08 The General Hospital Corporation Dispositif microfluidique pour la separation de cellules et utilisations de ce dispositif
WO2015175562A1 (fr) * 2014-05-15 2015-11-19 Kellbenx Incorporated Préparation de globules rouges nucléées fœtales (nrbc) pour test de diagnostic

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WO1993008269A1 (fr) * 1991-10-23 1993-04-29 Cellpro, Incorporated Procede pour enrichir des cellules souches f×tales issues du sang maternel
DK0720624T3 (da) * 1993-09-22 1999-08-09 Medical Res Council Omdirigering af antistoffer
AU2003901668A0 (en) * 2003-03-28 2003-05-01 Medvet Science Pty. Ltd. Non-haemopoietic precursor cells
US20060105353A1 (en) * 2004-11-16 2006-05-18 Jalal Syed M Non-invasive prenatal fetal cell diagnostic method
US7439062B2 (en) * 2004-12-23 2008-10-21 Biocept, Inc. Beads for capturing target cells from bodily fluid
US20070196820A1 (en) * 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US20070059781A1 (en) * 2005-09-15 2007-03-15 Ravi Kapur System for size based separation and analysis
JP2009511001A (ja) * 2005-09-15 2009-03-19 アルテミス ヘルス，インク． 細胞及びその他の粒子を磁気濃縮するためのデバイス並びに方法
CN101358241A (zh) * 2008-09-16 2009-02-04 山东亚大药业有限公司 一种用有核红细胞进行唐氏综合症产前基因筛查的方法和试剂盒
US20100304978A1 (en) * 2009-01-26 2010-12-02 David Xingfei Deng Methods and compositions for identifying a fetal cell
US20140141997A1 (en) * 2011-06-30 2014-05-22 National University Of Singapore Foetal nucleated red blood cell detection
CN103103161A (zh) * 2013-02-05 2013-05-15 山东亚大药业有限公司 胎儿有核红细胞分离纯化方法及唐氏综合症筛查试剂盒
US20160053317A1 (en) * 2013-05-16 2016-02-25 Basetra Medical Technology Co. Ltd. Fetal diagnostics using fetal cell capture from maternal blood
CN103602632A (zh) * 2013-11-15 2014-02-26 邯郸市康业生物科技有限公司 孕妇外周血中胎儿有核红细胞的分离方法
US20160130553A1 (en) * 2014-05-15 2016-05-12 Kellbenx Incorporated PREPARATION OF FETAL NUCLEATED RED BLOOD CELLS (NRBCs) FOR DIAGNOSTIC TESTING
CN105063181A (zh) * 2015-07-03 2015-11-18 石莹 从孕妇外周循环血中分离胎儿有核红细胞用于无创产前诊断
CN104977284B (zh) * 2015-07-03 2018-05-29 石莹 一种胎儿有核红细胞的捕获及鉴定方法
CN111500534B (zh) * 2016-07-11 2022-11-29 山东亚大药业有限公司 分离纯化胎儿有核红细胞的试剂盒

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WO2004029221A2 (fr) * 2002-09-27 2004-04-08 The General Hospital Corporation Dispositif microfluidique pour la separation de cellules et utilisations de ce dispositif
WO2015175562A1 (fr) * 2014-05-15 2015-11-19 Kellbenx Incorporated Préparation de globules rouges nucléées fœtales (nrbc) pour test de diagnostic

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CN111500534A (zh) 2020-08-07
WO2018010632A1 (fr) 2018-01-18
CN111500534B (zh) 2022-11-29
CN106119189A (zh) 2016-11-16

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