CN106110173A - A kind of Chinese medicine extract treating cyclomastopathy and extracting method thereof and application - Google Patents

A kind of Chinese medicine extract treating cyclomastopathy and extracting method thereof and application Download PDF

Info

Publication number
CN106110173A
CN106110173A CN201610506118.0A CN201610506118A CN106110173A CN 106110173 A CN106110173 A CN 106110173A CN 201610506118 A CN201610506118 A CN 201610506118A CN 106110173 A CN106110173 A CN 106110173A
Authority
CN
China
Prior art keywords
extract
alcohol
chinese medicine
radix curcumae
extraction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610506118.0A
Other languages
Chinese (zh)
Inventor
张军龙
濮存海
贾晓波
王伏军
马少平
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NINGXIA GOLD SUN PHARMACEUTICAL CO Ltd
Original Assignee
NINGXIA GOLD SUN PHARMACEUTICAL CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NINGXIA GOLD SUN PHARMACEUTICAL CO Ltd filed Critical NINGXIA GOLD SUN PHARMACEUTICAL CO Ltd
Priority to CN201610506118.0A priority Critical patent/CN106110173A/en
Publication of CN106110173A publication Critical patent/CN106110173A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/906—Zingiberaceae (Ginger family)
    • A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/24—Mucus; Mucous glands; Bursa; Synovial fluid; Arthral fluid; Excreta; Spinal fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/22—Anacardiaceae (Sumac family), e.g. smoketree, sumac or poison oak
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/752—Citrus, e.g. lime, orange or lemon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Inorganic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Neurology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a kind of Chinese medicine extract treating cyclomastopathy and its preparation method and application, this Chinese medicine extract is prepared by the following method and obtains: 1) use CO2Volatile oil in Radix Curcumae is preferentially proposed by supercritical technology, takes Radix Curcumae medicinal residues；2) Radix Curcumae medicinal residues, Herba Agrimoniae, Fructus Aurantii (parched with bran) use alcohol extraction according to its effective ingredient character, obtain alcohol-extracted extract；3) Alumen, Sal Nitri, Oletum Trogopterori, Resina Toxicodendri use water to carry, and obtain the water extracted immersing paste；4) the water extracted immersing paste, alcohol-extracted extract mix.The present invention makes effective site or effective ingredient and auxiliary element is as much as possible extracts, it is to avoid the invalid components such as heavy metal and suck tissue, reduces the injury to human body.Chinese medicine composition of the present invention has the effects such as depressed liver-energy dispersing and QI regulating, dissipating phlegm and resolving masses, regulating qi and dredging collateral, Chong and Ren Meridians regulating and nurses one's health endocrine effect, can treat or assist treatment cyclomastopathy.

Description

A kind of Chinese medicine extract treating cyclomastopathy and extracting method thereof and application
Technical field
The present invention relates to technical field of Chinese medicines, in particular to being a kind of Chinese medicine extract treating cyclomastopathy and extraction thereof Methods and applications.
Background technology
The pathogenesis of cyclomastopathy: motherland's traditional medicine thinks that generation cyclomastopathy is appointed relevant with liver,spleen,kidney, punching, many Having mechanism of qi obstructed, blood stasis, expectorant are solidifying, make the strongly fragrant resistance of meridian qi and blood be agglomerated into caused by core.Ancients think " disease of married woman arises from strongly fragrant more ", Owing to women easily suffers from stagnation of liver-QI for the psychological characteristics of feelings will change.Meanwhile, motherland's medical science thinks that gas and blood are closely bound up, gas Handsome for blood, gas row then blood, the stagnation of QI then blood stasis, hematogenous blockage then mammary gland changes therewith, thus easily forms liver energy stagnation type breast Gland hypertrophy is sick.
The important pathogenesis of cyclomastopathy is stagnation of QI due to depression of the liver liver controlling conveyance and dispersion, and irritability is the happiest and bar reaches, liver being bold and firm viscera, and body the moon is used Sun preferably rises and sends out and evacuate.Irritability had both disliked depression, also avoided high.Owing to feelings will is unsuccessful, melancholy puzzled, long strongly fragrant impairing the liver, or by essence God stimulates, irritable angry, all may result in depression of liver-QI, and the mechanism of qi stasis of blood is stagnant, pents up in breast stomach network, and breast network meridian and vessels obstruction is obstructed, no General rule causes mastalgia bitterly.
The treatment of cyclomastopathy can use blood circulation promoting and blood stasis dispelling and dissipating phlegm and resolving masses method, and activating blood and removing stasis Method is the conventional side for the treatment of nodules of the breast Method.The traditional Chinese medical science has saying of " pathogen usually intruding into collateral in protracted disease ", and nodules of the breast onset is slow, and the course of disease is long, touching does not heals, therefore lump in breast is to tie in breast for accumulation of phlegm in the hypochondrium Caused by network.Qi depression to blood stasis, breast network blocks, is the basic pathogenesis of cyclomastopathy." addiction " " knot " " bitterly " is the sick total clinic of breast Feature, no matter the breast of which kind of pattern of syndrome is sick, on the basis of differentiation of symptoms and signs for classification of syndrome treats, all needs the method for R. concomitans blood circulation promoting and blood stasis dispelling to dredge gas Blood, makes addiction blood agglomeration blockization dissipate, and breast network is unobstructed and swells and ache from disappearing.Modern medicine study result also confirms that invigorating blood circulation addiction method can change Being apt to or correct blood circulation and microcirculation disturbance, drug for invigorating blood circulation and eliminating stasis has reduction blood viscosity, accelerates the effects such as blood flowing, Thus promote the lump of hypertrophy and fiber to absorb.
At present, the treatment to cyclomastopathy is not had to have the product of remarkable result；Existing procucts are general not by its effective ingredient Extract largely, also or heavy metal and harmful element content the highest.
Summary of the invention
Present invention aim to provide the Chinese medicine extract of a kind of effective treatment cyclomastopathy.This Chinese medicine extract rises To effect conditioning endocrine such as depressed liver-energy dispersing and QI regulating, dissipating phlegm and resolving masses, regulating qi and dredging collateral, Chong and Ren Meridians regulating, fundamentally disintegrate cyclomastopathy Primary factors, adds that TAM, to estrogen antagonist, blocks its effect making gland tissue hyperplasia and edema, releases pain and swollen Completely wait discomfort, to reach effect for the treatment of both the principal and secondary aspects of a disease.
It is a further object of the present invention to provide the Chinese medicine extract preparation method of this treatment cyclomastopathy.
It is an object of the invention to be accomplished by:
A kind of Chinese medicine extract treating cyclomastopathy, is prepared by the following method and obtains:
Take Radix Curcumae 54 weight portion, Herba Agrimoniae 54 weight portion, Oletum Trogopterori 45 weight portion, Alumen 54 weight portion, Sal Nitri 54 weight Part, Resina Toxicodendri 18 weight portion, stir-baked Fructus Aurantii in bran 90 weight portion；
Above-mentioned raw materials is prepared in such a way:
(1) Radix Curcumae is through CO2Supercritical extraction obtains Radix Curcumae extract and Radix Curcumae raffinate；
(2) Radix Curcumae raffinate step (1) obtained and stir-baked Fructus Aurantii in bran and Herba Agrimoniae mixing alcohol extraction, filters, obtains alcohol extraction Medicinal residues and filtrate, filtrate concentrates, obtains alcohol-extracted extract；
(3) alcohol extraction medicinal residues step (2) obtained carry with Resina Toxicodendri, Oletum Trogopterori, Alumen and Sal Nitri mixing water, filter, obtain Water carries medicinal residues and filtrate, and filtrate concentrates, and obtains the water extracted immersing paste；
(4) the water extracted immersing paste that alcohol-extracted extract step (2) obtained obtains with step (3) mixes.
CO described in step (1)2Supercritical extraction condition is: extracting pressure is 25-35MPa, extraction temperature 25-35 DEG C, CO2Flow 11-15L/h, extraction time 1-2h.
CO described in preferred steps (1)2Supercritical extraction condition is: extracting pressure is 35MPa, extraction temperature 30 DEG C, CO2 Flow 13L/h, extraction time 1.5h.Separating still I pressure 10～11MPa, temperature 48 DEG C；Separating still II pressure 7～8MPa, temperature 36℃。
Alcohol extraction condition described in step (2) is: add stir-baked Fructus Aurantii in bran, Herba Agrimoniae and three kinds of mixture 6-10 of Radix Curcumae raffinate Times weight 70%-90% alcohol reflux 1-3 time, each 1-2 hour, backflow stood more than 12 hours, Aspirate supernatant, 300 mesh filter, and filtrate is evaporated to the alcohol-extracted extract of relative density 1.15～1.25 under the conditions of 60 DEG C.
Preferred steps (2) specifically includes following steps: takes the stir-baked Fructus Aurantii in bran of recipe quantity, Herba Agrimoniae and Radix Curcumae raffinate and adds three Planting 8 times of weight 80% alcohol reflux of mixture twice, each 2 hours, backflow stood more than 12 hours, drew supernatant Liquid, filters, the clear paste of relative density 1.15～1.25 under the conditions of filtrate reduced in volume to 60 DEG C.
Described step (3) specifically includes following steps: take the Resina Toxicodendri of recipe quantity, Oletum Trogopterori, Alumen, Sal Nitri and alcohol extraction medicine Slag, adds water reflux, extract, 1-3 time of 6-10 times of weight of five kinds of mixture, and each 1-2 hour, backflow stood more than 12 hours, Aspirate supernatant, 300 mesh filter, and filtrate is concentrated under reduced pressure to give the water of relative density 1.15～1.25 under the conditions of 60 DEG C and proposes leaching Cream；
Preferred steps (3) specifically includes following steps: take the Resina Toxicodendri of recipe quantity, Oletum Trogopterori, Alumen, Sal Nitri and alcohol extraction medicine Slag, adds the water reflux, extract, 2 times of 10 times of weight of five kinds of mixture, and each 1.5 hours, backflow stood more than 12 hours, drew Supernatant, filters, the clear paste of relative density 1.15～1.25 under the conditions of filtrate reduced in volume to 60 DEG C.
Dosage form made by described Chinese medicine extract and pharmaceutically acceptable adjuvant, preferred dosage form be tablet, capsule, Granule, pill.
The preparation method of the Chinese medicine extract of above-mentioned treatment cyclomastopathy comprises the following steps:
Take Radix Curcumae 54 weight portion, Herba Agrimoniae 54 weight portion, Oletum Trogopterori 45 weight portion, Alumen 54 weight portion, Sal Nitri 54 weight Part, Resina Toxicodendri 18 weight portion, stir-baked Fructus Aurantii in bran 90 weight portion；
Above-mentioned raw materials is prepared in such a way:
(1) Radix Curcumae is through CO2Supercritical extraction obtains Radix Curcumae extract and Radix Curcumae raffinate, takes Radix Curcumae raffinate；
(2) Radix Curcumae raffinate, stir-baked Fructus Aurantii in bran and Herba Agrimoniae mixing alcohol extraction step (1) obtained, filters, obtains alcohol extraction medicine Slag and filtrate, filtrate concentrates, obtains alcohol-extracted extract；
(3) alcohol extraction medicinal residues step (2) obtained carry with Resina Toxicodendri, Oletum Trogopterori, Alumen and Sal Nitri mixing water, filter, obtain Water carries medicinal residues and filtrate, and filtrate concentrates, and obtains the water extracted immersing paste；
(4) the water extracted immersing paste that alcohol-extracted extract step (2) obtained obtains with step (3) mixes and get final product.
The Chinese medicine extract for the treatment of cyclomastopathy of the present invention is in preparation treatment or prevention or assists treatment mammary gland to increase Raw medicine is applied.
Divide in effect conditionings such as Chinese medicine extract function depressed liver-energy dispersing and QI regulating of the present invention, dissipating phlegm and resolving masses, regulating qi and dredging collateral, Chong and Ren Meridians regulating Secrete.
In this prescription, the pharmacology of each medicine is:
Radix Curcumae is zingiberaceous plant RADIX CURCUMAE (Cur cuma weny uj in Y.H.Chenet C.Ling), Rhizoma Curcumae Longae (Curclma longa L.), Guangxi zedoary (Curcumakw angsiensis S.G.Lee et C.F.Liang) or fluffy cowherb The dried root of art (Curcuma haeocaulis Val.).The above two practise respectively title " RADIX CURCUMAE " with " Radix Curcuma ", remaining Practise by character difference and claim osmanthus Radix Curcumae or green filament Radix Curcumae.Radix Curcumae mainly originates in the ground such as Sichuan, Fujian, Zhejiang, Guangxi.In Radix Curcumae mainly Containing compositions such as volatile oil, curcumin, polysaccharide, a small amount of trace element, wherein volatile oil is Radix Curcumae antineoplastic effective composition, Rhizoma Curcumae Longae Element is the principle active component of Rhizoma Curcumae blood fat reducing, antioxidation, antiinflammatory.Modern pharmacology research shows, it is thin that Radix Curcumae has protection liver Born of the same parents, promotion liver cell regeneration, anticancer, antibacterial, antioxidative effect.Volatile oil and curcumin are the principle active component of Radix Curcumae. Radix Curcumae volatile oil not only has the effect of sterilization, antiviral, antiinflammatory and blood circulation promoting and blood stasis dispelling, removing the necrotic tissue and promoting granulation, and may also suppress tumor The growth of cell, has positive role to prevention cervical cancer.Curcumin has the multiple pharmacology such as antitumor, antiinflammatory, antibacterial, antioxidation Effect, and toxicity is low, has good clinical practice potentiality, is paid close attention to widely by both at home and abroad.Extracting method of the present invention can fill Divide the curcumin proposed in Radix Curcumae and effectively utilize its volatile oil component, life can be improved while increasing the stability of medicine Thing availability.
Fructus Aurantii is rutaceae Citrus aurantium Linn. (Citrus aurantium L1) and the dry crude fruit of variety thereof Real.Bitter in the mouth, pungent, sour.Cold nature.Return spleen, stomach warp.There is regulating the flow of QI to ease the stomach, effect of the stagnant relieving distension of row.The stagnation of QI, distension is coerced for breast Pain, accumulation of food in the stomach and intes tine due to indigestion, stops in phlegm retention, visceroptosis.Fructus Aurantii is one of typical QI regulating medicine of Chinese medicine, is widely used in TCM Bed.Contained by Fructus Aurantii, chemical composition is complex, the effective ingredient being currently known mainly have flavonoid, volatile oil, alkaloids, Coumarins and some trace element etc..From the point of view of PINGXIAO PIAN is used for treating cyclomastopathy, we mainly take Fructus Aurantii Hesperidin, naringin and Nobiletin in flavones ingredient.Hesperidin has antiinflammatory action, antitumaous effect.Naringin has Anti-inflammatory and analgesic effect.Nobiletin has the pharmacological actions such as antiinflammatory, antiviral, antioxidation.Because the flavones ingredient in Fructus Aurantii It is insoluble in water.
Herba Agrimoniae is the ground drying nest of rosaceous plant Radix Agrimoniae (Agrimonia Pilosa Ledeb), mainly becomes Dividing and have flavonoid, agrimonine, agrimonolide, tannin, volatile oil, organic acid and agrimophol etc., property is bitter, puckery, GUIXIN, Liver Channel, has astringing to arrest bleeding, preventing the attack (or recurrence) of malaria, dysentery relieving, Detoxication, can be used for treat hemoptysis, haematemesis, metrostaxis, malaria, dysentery, De-power impairment caused by overstrain, carbuncle sore tumefacting virus, pudendal pruritus leukorrhagia.Flavonoid, Polyphenols and lactone component are insoluble in water.
Resina Toxicodendri (dried lacquer) is Anacardiaceae (Anacar diaceae) plant Toxicodendron verniciflnum (Stokes) F. A. Barkley (Rhus verniciflua Stokes) Tox icodend ron v The Resina Toxicodendri verniciflui drying of ernicif luum (Sto kes) F1A1Barkley secretion processes.Resina Toxicodendri has another name called paint slag, the paint end, paint Foot, continuous life cylinder, pitch-dark, China goes through an edition pharmacopeia and all includes；Have removing blood stasis with potent drugs blood, disappear the effect such as blood, parasite killing, is mainly used in blood stasis, worm The treatment of the diseases such as long-pending stomachache, women's amenorrhea.Being used for antineoplastic principle active component in Resina Toxicodendri is urushipolysaccharide, and urushipolysaccharide has Various biological activity, such as antitumor, anti-H IV, blood coagulation resisting function etc..Urushipolysaccharide is a kind of glucosides constituents, soluble in water.
Oletum Trogopterori (Faeces trogopterori) is flying squirrel section animal trogopterus xanthipes Milne-Edwards (Trogoterus xanthip Es) dry feces, function is blood circulation promoting and blood stasis dispelling, pain relieving, and applicating history is long, and clinically have in the dysmenorrhea caused by blood stasis, the heart more The diseases such as angor.It is reported, the water decoction of Oletum Trogopterori has inhibitory action to platelet aggregation, and Oletum Trogopterori water extract is external can substantially be pressed down Rabbit platelet aggregation processed, its inhibitory action is relevant to dosage, and Oletum Trogopterori has fibrinolysis activity, Oletum Trogopterori ethyl acetate extract With Oletum Trogopterori water decoction, also there is antiinflammatory action.The water extract of Oletum Trogopterori can suppress platelet aggregation.
Described in " China's book on Chinese herbal medicine ", Sal Nitri has used in " 52 Bingfang "；Acrid in the mouth, hardship are micro-salty；GUIXIN, spleen, Lung meridian.Assaulting fortified position removing mass, under diuresis is rushed down, removing toxic substances and promoting subsidence of swelling, cure mainly heatstroke wound cold, abdominal distention is vomited and diarrhoea, trusted subordinate's pain, jaundice, the puckery pain of all pouring, Sore throat, conjunctival congestion with ophthalmalgia swells, furunculosis.Containing heavy metal arsenic in Sal Nitri, arsenic is insoluble in water, and the present invention is in order to cut down the toxicity of arsenic, to nitre Stone uses water to carry.
Alumen is mainly composed of aqueous sulfuric acid aluminum potassium [KAl (SO4)2·12H2O].Head is loaded in Shennong's Herbal, originates in Zhejiang The ground such as river, Anhui, Gansu, Hubei, Shanxi.For the alum crystallisation of the Sulfates processed refinement of mineral alunite, belong to Trigonal system, have eliminate the phlegm, dampness, hemostasis, antidiarrheal, anticorrosion, effect of sterilization.In Alumen containing a small amount of trace element-selenium and Zinc, selenium can suppress the propagation of tumor cell；Zinc has suppression carcinogen and lures the effect of cancer.Sal Nitri and Alumen are mainly composed of salt Class, and use water extraction to eliminate the heavy metal impact on medicine containing heavy metal and the arsenic salt present invention.
Chinese medicine extract of the present invention has the merits such as blood circulation promoting and blood stasis dispelling, pain relieving eliminating stagnation, heat-clearing and toxic substances removing, strengthening vital QI to eliminate pathogenic factors, qi-restoratives dredging collateral Effect, the tuberosity for each phase hypertrophy all has good dissipation role.Utilize extract depressed liver-energy dispersing and QI regulating of the present invention, dissipating phlegm and resolving masses, reason Effect conditioning endocrine such as gas dredging collateral, Chong and Ren Meridians regulating, fundamentally disintegrate the Primary factors of cyclomastopathy, add that TAM antagonism is female Hormone, blocks its effect making gland tissue hyperplasia and edema, releases the discomfort such as pain and distension, to reach treating both the principal and secondary aspects of a disease Effect.Additionally, also cyclomastopathy tuberosity there to be good eliminating stagnation effect.
The invention have the advantages that Resina Toxicodendri, Oletum Trogopterori, Alumen and Sal Nitri use water to carry, make effective site or effective Composition and auxiliary element is as much as possible extracts, and the invalid components such as heavy metal and suck tissue do not extract Come, reduce the injury to human body.The present invention treats the Chinese medicine extract of cyclomastopathy and can make various dosage form and (include taking orally Hard capsule, soft capsule, tablet, granule, drop pill).
The know-why of the present invention and technical study:
One, extraction process by water research
1, orthogonal test affects the principal element of extraction process by water and adds water times amount, extraction time and extraction time.Above-mentioned 3 Factor respectively takes 3 levels and carries out L9(34) orthogonal test, take alcohol extraction medicinal residues, Resina Toxicodendri, Oletum Trogopterori, Alumen and the Sal Nitri of recipe quantity by Following factor level table extracts, test arrangement and the results are shown in Table 1.
The quadrature factor water-glass of table 1. abstract methods
The present invention select zinc, ferrum, magnesium, selenium total amount i.e. beneficial element content (/), the i.e. harmful unit of arsenic, chromium, lead, total amount Cellulose content (/) and dry spun (%) be inspection target, orthogonal experiment is carried out data analysis.Orthogonal calendar and result It is shown in Table 2.
Table 2. water carries the orthogonal arrangement of test and result table
(table provides 9 experimental programs.If No. 6 experiment is A2B3C1, water reflux, extract, 2h of i.e. 8 times amount, extract 1 Secondary, the rest may be inferred)
With beneficial element as index, range analysis result B > C > A, the best of breed between each factor and level is A1B3C3, With A item as error term, carry out variance analysis further, the results are shown in Table 3.
The table 3. analysis of variance table with beneficial element total amount as index
(F0.05(2,2)=19.00) with beneficial element content as index, each factor on experimental result there are no significant impact, Preferably optimised process is A1B3C3。
With harmful element content as index, range analysis result B > A > C.Best of breed between each factor and level is A3B2C1, with C item as error term, carry out variance analysis further, the results are shown in Table 4.
The table 4. analysis of variance table with harmful element content as index
(F0.05(2,2)=19.00) with harmful element content as index, experimental result is had a significant impact by solvent times amount, Preferably optimised process is A3B2C1。
With dry spun as index, range analysis result A > B > C.Best of breed between each factor and level is A2B3C2, With C item as error term, carry out variance analysis further, the results are shown in Table 5.
The table 5. analysis of variance table with dry spun as index
(F0.05(2,2)=19.00) with dry spun as index, each level on experimental result there are no significant impact, preferably Optimised process be A2B3C2。
For the orthogonal result of more rational evaluation, find optimum extraction process, three evaluation indexes are carried out comprehensive grading, calculate Method is as follows: in three indexs, chooses soprano respectively as 100 points, and remaining is pressed formula and calculates: score Y=weight × (100 peak+yi), to three index weights coefficients respectively for for 0.3,0.3 and 0.4.Analyzed by directly perceived, with comprehensively Scoring is index, show that each factor is B > A > C on extracting result impact order, and the best of breed between each factor and level is A3B2C2, with C item as error term, carry out variance analysis the results of analysis of variance further and be shown in Table 6.
Table 6. comprehensive grading is the analysis of variance table of index
(F0.05(2,2)=19.00) with comprehensive grading as index, experimental result is affected by each factor without significance, Good technique is A3B2C2。
1, water carries confirmatory experiment
In order to verify the above results, take the above-mentioned medical material of 0.1 times of recipe quantity, according to A3B2C2Condition carries out water and proposes experiment and test Card, result such as table 7
Table 7 water carries confirmatory experiment result table
The result of confirmatory experiment is consistent substantially with Orthogonal Parameter, i.e. water carry the extraction conditions of experiment be amount of water be 10 times, Extract twice, each 1.5h.
We carry out mammary gland therapeutic experiment to the raw material components side of tearing open of the present invention, effect of the most each prescription, three Prescription is respectively as follows:
Prescription 1: of the present invention group (alcohol-extracted extract+the water extracted immersing paste): Radix Curcumae 54 weight portion is through CO2Supercritical extraction, obtains raffinate Thing；Raffinate and 54 weight portion Herba Agrimoniaes and 90 parts of stir-baked Fructus Aurantii in bran mix homogeneously, add the ethanol extraction twice of 8 times amount 80%, 2h every time, backflow stands more than 12 hours, and Aspirate supernatant filters, relative density 1.15 during filtrate reduced in volume to 60 DEG C ～the clear paste of 1.25, obtain alcohol-extracted extract.Alcohol extraction medicinal residues and 45 weight portion Oletum Trogopteroris, 54 weight portion Alumens, 54 weight portion Sal Nitri, 18 Weight portion Resina Toxicodendri adds the water reflux, extract, twice of 10 times of weight of five kinds of mixture, each 1.5 hours, backflow stand 12 hours with On, Aspirate supernatant, filter, the clear paste of relative density 1.15～1.25 under the conditions of filtrate reduced in volume to 60 DEG C；The water extracted immersing paste+ Alcohol-extracted extract is prescription 1.
Prescription 2: Herba Agrimoniae (alcohol extraction+water carries): 54 weight portion Herba Agrimoniaes add the ethanol extraction twice of 8 times amount 80%, often Secondary 2h, backflow stand more than 12 hours, Aspirate supernatant, filter, during filtrate reduced in volume to 60 DEG C relative density 1.15～ The clear paste of 1.25, obtains alcohol-extracted extract.Alcohol extraction medicinal residues add the water reflux, extract, twice of 10 times of weight, and each 1.5 hours, backflow was quiet Putting more than 12 hours, Aspirate supernatant, filter, under the conditions of filtrate reduced in volume to 60 DEG C, relative density 1.15～1.25 is clear Cream；The water extracted immersing paste+alcohol-extracted extract is prescription 2.
Prescription 3: stir-baked Fructus Aurantii in bran (alcohol extraction+water carries): 90 parts of stir-baked Fructus Aurantii in bran add the ethanol extraction twice of 8 times amount 80%, often Secondary 2h, backflow stand more than 12 hours, Aspirate supernatant, filter, during filtrate reduced in volume to 60 DEG C relative density 1.15～ The clear paste of 1.25, obtains alcohol-extracted extract.Alcohol extraction medicinal residues add the water reflux, extract, twice of 10 times of weight, and each 1.5 hours, backflow was quiet Putting more than 12 hours, Aspirate supernatant, filter, under the conditions of filtrate reduced in volume to 60 DEG C, relative density 1.15～1.25 is clear Cream；The water extracted immersing paste+alcohol-extracted extract is prescription 3.
1, female non-pregnant rat about the 220g of laboratory animal: SD 80
2, reagent: estradiol benzoate injection specification 2g.L-1/, Shanghai General Pharmaceutical Co., ltd.
Lot number 150501
Estradiol (E2), progesterone (P) test kit all has Beijing China English biotechnology research to be produced
Lot number 151012
Slide gauge Shenzhen Xin Baolai detecting instrument company limited
Refrigerated centrifuger Beijing epoch Bei Li centrifuge company limited
R 911 Full automatic exempts from industry head office of calculating instrument Chinese University of Science and Technology
3, method
3.1 take 50 SD rats is randomly divided into 5 groups, model group (normal saline 15ml/), blank group (etc. Amount normal saline 15ml/), prescription 1 (2.4g/), prescription 2 (2.4g/), prescription 3 (2.4g/).Blank group is big Mus intramuscular injection normal saline (0.05ml/ only), once a day, each model group rats intramuscular injection estradiol benzoate 0.5/ , extremely experiment terminates once a day.Modeling started gastric infusion after 1 week, every day 1 time, was administered 4 weeks, and last is drawn materials after being administered 2h.
The forward and backward slide gauge of mensuration modeling of 3.2 cyclomastopathy accurately measures rat the 2nd to 2, left and right breast diameter And height of nipples, and observe its outward appearance.
Fasting 12h before 3.3 hormone determination experiments, after last is administered 2h, abdominal aortic blood 5mL, 3000r min-1High Speed frozen centrifugation 15min, takes serum and adds the cold petroleum ether of 4mL, recentrifuge after mixing.Phase is abandoned in suction, after lower nitrogen dries up It is dried and seals, to be measured.
Use r 911 Full automatic to exempt from calculating instrument and radioimmunoassay kits, by product description, serum is carried out female Glycol (E2), progesterone (P) detection.
4 results
4.1 on papilla diameter, height of nipples impact
Compared with normal group, model group rats papilla diameter and highly significant increase, and nipple holds up hyperemia.In injection female two Alcohol reaches swelling peak on the 2nd week, and within the 3rd week, swelling maintains an equal level, and begins to decline at the 4th week.Prescription 1, prescription 2,3 groups of nipples of prescription are straight Footpath, height are compared with model group and to be substantially reduced.Result shows, the cyclomastopathy that estradiol is induced is had and significantly presses down by this extract Make and use.
The change of table 8 rat papilla diameter (N=10)
Compare with blank groupbP<0.001；Compare with model control groupcP<0.001；dP<0.05；Compare with prescription 1fP<0.05；
The change of table 9 rat height of nipples (N=10)
Compare with blank groupbP<0.001；Compare with model control groupcP<0.001；dP<0.01；eP<0.05；With group Side 1 comparesfP<0.01；
4.2 couples of serum estradiol (E2), the impact of progesterone (P) content
Testing result shows, positive controls and PINGXIAO PIAN high dose group relatively model group estrogen level decrease, pregnant Ketone level raises, and result see table
Table 10 rat is to serum estradiol (E2), the change of progesterone (P) content (N=10)
Compare with blank groupbP<0.001；Compare with model control groupcP<0.001；dP<0.01；Compare with prescription 1eP<0.05；
5. discuss
Being illustrated by above results of animal, extract of the present invention has the effect for the treatment of cyclomastopathy, improves The quality of life of patient, and any toxic and side effects and untoward reaction do not occur in experimentation.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1:
Radix Curcumae 54g, Herba Agrimoniae 54g, Oletum Trogopterori 45g, Alumen 54g, Sal Nitri 54g, Resina Toxicodendri 18g, stir-baked Fructus Aurantii in bran 90g, Semen Strychni Sub-powder 36g；
The preparation method of extract tablet of the present invention, it comprises the steps:
(1) Radix Curcumae is taken through CO2Supercritical extraction obtains extract and raffinate.CO2Supercritical extraction condition is: extracting pressure For 35MPa, extraction temperature 30 DEG C, CO2Flow 13L/h, extraction time 1.5h.Separating still I pressure 10～11MPa, temperature 48 DEG C； Separating still II pressure 7～8MPa, temperature 36 DEG C.
(2) alcohol extraction: take the stir-baked Fructus Aurantii in bran of recipe quantity, Herba Agrimoniae and Radix Curcumae raffinate and add 8 times amount 80% alcohol refluxs and carry Taking twice, each 2 hours, backflow stands more than 12 hours, Aspirate supernatant, and 300 mesh filter, and filtrate subtracts under the conditions of 60 DEG C Pressure is concentrated into the alcohol-extracted extract of relative density 1.15～1.25, standby；
(3) water carries: taking alcohol extraction medicinal residues in the Resina Toxicodendri of recipe quantity, Oletum Trogopterori, Alumen, Sal Nitri and (2), the water adding 10 times amount returns Stream extraction twice, each 1.5 hours, backflow stood more than 12 hours, Aspirate supernatant, and 300 mesh filter, and filtrate is at 60 DEG C of bars The water extracted immersing paste being concentrated under reduced pressure to give relative density 1.15～1.25 under part is standby；Recording beneficial element content is 3.35g/, Harmful element content is 81.67mg/, and dry spun is 28.45%.
(4) the extractum mixing drying that step (2) obtains with step (3) is obtained dry cream and get final product.
Embodiment 2:
Radix Curcumae 54g, Herba Agrimoniae 54g, Oletum Trogopterori 45g, Alumen 54g, Sal Nitri 54g, Resina Toxicodendri 18g, stir-baked Fructus Aurantii in bran 90g, Semen Strychni Sub-powder 36g；
The preparation method of extract tablet of the present invention, it comprises the steps:
(1) Radix Curcumae is taken through CO2Supercritical extraction obtains extract and raffinate.CO2Supercritical extraction condition is: extracting pressure For 35MPa, extraction temperature 30 DEG C, CO2Flow 13L/h, extraction time 1.5h.Separating still I pressure 10～11MPa, temperature 48 DEG C； Separating still II pressure 7～8MPa, temperature 36 DEG C.
(2) alcohol extraction: take the stir-baked Fructus Aurantii in bran of recipe quantity, Herba Agrimoniae and Radix Curcumae raffinate and add 8 times amount 90% alcohol refluxs and carry Taking twice, each 1.5 hours, backflow stands more than 12 hours, Aspirate supernatant, and 300 mesh filter, and filtrate is under the conditions of 60 DEG C It is evaporated to the alcohol-extracted extract of relative density 1.15～1.25, standby；
(3) water carries: taking alcohol extraction medicinal residues in the Resina Toxicodendri of recipe quantity, Oletum Trogopterori, Alumen, Sal Nitri and (2), the water adding 10 times amount returns Stream extraction twice, each 2 hours, backflow stood more than 12 hours, Aspirate supernatant, and 300 mesh filter, and filtrate is 60 DEG C of conditions Under to be concentrated under reduced pressure to give the water extracted immersing paste of relative density 1.15～1.25 standby；Recording beneficial element content is 3.42g/, has Evil constituent content is 80.87mg/, and dry spun is 27.91%.
(4) the extractum mixing drying that step (2) obtains with step (3) is obtained dry cream and get final product.
Embodiment 3:
Radix Curcumae 54g, Herba Agrimoniae 54g, Oletum Trogopterori 45g, Alumen 54g, Sal Nitri 54g, Resina Toxicodendri 18g, stir-baked Fructus Aurantii in bran 90g, Semen Strychni Sub-powder 36g；
The preparation method of extract tablet of the present invention, it comprises the steps:
(1) Radix Curcumae is taken through CO2Supercritical extraction obtains extract and raffinate.CO2Supercritical extraction condition is: extracting pressure For 35MPa, extraction temperature 30 DEG C, CO2Flow 13L/h, extraction time 1.5h.Separating still I pressure 10～11MPa, temperature 48 DEG C； Separating still II pressure 7～8MPa, temperature 36 DEG C.
(2) alcohol extraction: take the stir-baked Fructus Aurantii in bran of recipe quantity, Herba Agrimoniae and Radix Curcumae raffinate and add 10 times amount 70% alcohol refluxs and carry Taking twice, each 1.5 hours, backflow stands more than 12 hours, Aspirate supernatant, and 300 mesh filter, and filtrate is under the conditions of 60 DEG C It is evaporated to the alcohol-extracted extract of relative density 1.15～1.25, standby；
(3) water carries: taking alcohol extraction medicinal residues in the Resina Toxicodendri of recipe quantity, Oletum Trogopterori, Alumen, Sal Nitri and (2), the water adding 8 times amount returns Stream extraction twice, each 2 hours, backflow stood more than 12 hours, Aspirate supernatant, and 300 mesh filter, and filtrate is 60 DEG C of conditions Under to be concentrated under reduced pressure to give the water extracted immersing paste of relative density 1.15～1.25 standby；Recording beneficial element content is 3.29g/, has Evil constituent content is 79.81mg/, and dry spun is 29.05%.
(4) the extractum mixing drying that step (2) obtains with step (3) is obtained dry cream and get final product.
Embodiment 4
The broken rear adjuvants such as starch that add of dried cream powder prepared according to embodiment 1 method prepare according to common process Granule.
Embodiment 5
The broken rear adjuvants such as starch that add of dried cream powder prepared according to embodiment 1 method prepare according to common process Granule, then tabletting prepares tablet.
Embodiment 6
The broken rear adjuvants such as starch that add of dried cream powder prepared according to embodiment 1 method prepare according to common process Granule, then fill gelatine capsule leather obtains capsule.
Embodiment 7
The broken rear adjuvant that adds of dried cream powder prepared according to embodiment 1 method prepares honeyed pill according to common process.
Embodiment 8
The broken rear adjuvant that adds of dried cream powder prepared according to embodiment 1 method prepares drop pill according to common process.

Claims (10)

1. the Chinese medicine extract treating cyclomastopathy, it is characterised in that this Chinese medicine extract is to be prepared by the following method Arrive:
Take Radix Curcumae 54 weight portion, Herba Agrimoniae 54 weight portion, Oletum Trogopterori 45 weight portion, Alumen 54 weight portion, Sal Nitri 54 weight portion, do Paint 18 weight portions, stir-baked Fructus Aurantii in bran 90 weight portion；
Above-mentioned raw materials is prepared in such a way:
(1) Radix Curcumae is through CO2Supercritical extraction obtains Radix Curcumae extract and Radix Curcumae raffinate；
(2) Radix Curcumae raffinate step (1) obtained and stir-baked Fructus Aurantii in bran and Herba Agrimoniae mixing alcohol extraction, filters, obtains alcohol extraction medicinal residues And filtrate, filtrate concentrates, obtains alcohol-extracted extract；
(3) alcohol extraction medicinal residues step (2) obtained carry with Resina Toxicodendri, Oletum Trogopterori, Alumen and Sal Nitri mixing water, filter, obtain water and carry Medicinal residues and filtrate, filtrate concentrates, obtains the water extracted immersing paste；
(4) the water extracted immersing paste that alcohol-extracted extract step (2) obtained obtains with step (3) mixes.
The Chinese medicine extract for the treatment of cyclomastopathy the most according to claim 1, it is characterised in that the CO described in step (1)2Super Critical extraction conditions is: extracting pressure is 25-35MPa, extraction temperature 25-35 DEG C, CO2Flow 11-15L/h, extraction time 1- 2h。
The Chinese medicine extract for the treatment of cyclomastopathy the most according to claim 2, it is characterised in that the CO described in step (1)2Super Critical extraction conditions is: extracting pressure is 35MPa, extraction temperature 30 DEG C, CO2Flow 13L/h, extraction time 1.5h.
The Chinese medicine extract for the treatment of cyclomastopathy the most according to claim 1, it is characterised in that the alcohol extraction described in step (2) Condition is: adds stir-baked Fructus Aurantii in bran, Herba Agrimoniae and Radix Curcumae raffinate 6-10 times of weight 70%-90% alcohol reflux of three kinds of mixture and carries Taking 1-3 time, each 1-2 hour, backflow stands more than 12 hours, Aspirate supernatant, and 300 mesh filter, and obtain medicinal residues and filtrate； Filtrate is evaporated to the alcohol-extracted extract of relative density 1.15～1.25 under the conditions of 60 DEG C.
The Chinese medicine extract for the treatment of cyclomastopathy the most according to claim 4, it is characterised in that the alcohol extraction described in step (2) Condition is: add stir-baked Fructus Aurantii in bran, Herba Agrimoniae and 8 times of weight 80% alcohol reflux of three kinds of mixture of Radix Curcumae raffinate twice, often Secondary 2 hours, backflow stood more than 12 hours, Aspirate supernatant, filtered, the closeest under the conditions of filtrate reduced in volume to 60 DEG C The clear paste of degree 1.15～1.25.
The Chinese medicine extract for the treatment of cyclomastopathy the most according to claim 1, it is characterised in that described step (3) is concrete Comprise the following steps: take the Resina Toxicodendri of recipe quantity, Oletum Trogopterori, Alumen, Sal Nitri and alcohol extraction medicinal residues, add 6-10 times of weight of five kinds of mixture Water reflux, extract, 1-3 time, each 1-2 hour, backflow stood more than 12 hours, Aspirate supernatant, and 300 mesh filter, filtrate The water extracted immersing paste of relative density 1.15～1.25 it is concentrated under reduced pressure to give under the conditions of 60 DEG C.
The Chinese medicine extract for the treatment of cyclomastopathy the most according to claim 6, it is characterised in that described step (3) is concrete Comprise the following steps: take the Resina Toxicodendri of recipe quantity, Oletum Trogopterori, Alumen, Sal Nitri and alcohol extraction medicinal residues, add 10 times of weight of five kinds of mixture Water reflux, extract, 2 times, each 1.5 hours, backflow stood more than 12 hours, Aspirate supernatant, filtered, and filtrate reduced in volume is extremely The clear paste of relative density 1.15～1.25 under the conditions of 60 DEG C.
The Chinese medicine extract for the treatment of cyclomastopathy the most according to claim 1, it is characterised in that described Chinese medicine extract Making dosage form with pharmaceutically acceptable adjuvant, preferred dosage form is tablet, capsule, granule, pill.
9. the extracting method of the Chinese medicine extract of the treatment cyclomastopathy described in a claim 1, it is characterised in that the method Comprise the following steps:
Take Radix Curcumae 54 weight portion, Herba Agrimoniae 54 weight portion, Oletum Trogopterori 45 weight portion, Alumen 54 weight portion, Sal Nitri 54 weight portion, do Paint 18 weight portions, stir-baked Fructus Aurantii in bran 90 weight portion；
Above-mentioned raw materials is prepared in such a way:
(1) Radix Curcumae is through CO2Supercritical extraction obtains Radix Curcumae extract and Radix Curcumae raffinate；
(2) Radix Curcumae raffinate, stir-baked Fructus Aurantii in bran and Herba Agrimoniae mixing alcohol extraction step (1) obtained, filter, obtain alcohol extraction medicinal residues and Filtrate, filtrate concentrates, obtains alcohol-extracted extract；
(3) alcohol extraction medicinal residues step (2) obtained carry with Resina Toxicodendri, Oletum Trogopterori, Alumen and Sal Nitri mixing water, filter, obtain water and carry Medicinal residues and filtrate, filtrate concentrates, obtains the water extracted immersing paste；
(4) the water extracted immersing paste that alcohol-extracted extract step (2) obtained obtains with step (3) mixes and get final product.
10. the Chinese medicine extract of the treatment cyclomastopathy described in claim 1 in preparation treatment or prevention or assists treatment mammary gland Application in the medicine of hypertrophy.
CN201610506118.0A 2016-06-29 2016-06-29 A kind of Chinese medicine extract treating cyclomastopathy and extracting method thereof and application Pending CN106110173A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610506118.0A CN106110173A (en) 2016-06-29 2016-06-29 A kind of Chinese medicine extract treating cyclomastopathy and extracting method thereof and application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610506118.0A CN106110173A (en) 2016-06-29 2016-06-29 A kind of Chinese medicine extract treating cyclomastopathy and extracting method thereof and application

Publications (1)

Publication Number Publication Date
CN106110173A true CN106110173A (en) 2016-11-16

Family

ID=57468458

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610506118.0A Pending CN106110173A (en) 2016-06-29 2016-06-29 A kind of Chinese medicine extract treating cyclomastopathy and extracting method thereof and application

Country Status (1)

Country Link
CN (1) CN106110173A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110101823A (en) * 2019-06-19 2019-08-09 广州亿美化妆品生物科技有限公司 A kind of Chinese medical extract that treating the proliferation of mammary gland and its extracting method and application

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101461926A (en) * 2009-01-15 2009-06-24 郁慧 Medicament composition for treating gland hyperplasia and preparation method thereof
CN101612357A (en) * 2009-02-13 2009-12-30 西安正大制药有限公司 A kind of Chinese medicine composition for the treatment of tumor and preparation method thereof
CN101574506B (en) * 2008-05-29 2010-12-29 宁夏金太阳药业有限公司 Method for preparing pingxiao preparation
CN105477045A (en) * 2016-01-19 2016-04-13 宁夏金太阳药业有限公司 Traditional Chinese medicine composition containing chrysanthemum and wild chrysanthemum volatile oil and preparation method of traditional Chinese medicine composition
CN105535439A (en) * 2016-01-19 2016-05-04 宁夏金太阳药业有限公司 Traditional Chinese medicinal composition for treating pharyngitis and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101574506B (en) * 2008-05-29 2010-12-29 宁夏金太阳药业有限公司 Method for preparing pingxiao preparation
CN101461926A (en) * 2009-01-15 2009-06-24 郁慧 Medicament composition for treating gland hyperplasia and preparation method thereof
CN101612357A (en) * 2009-02-13 2009-12-30 西安正大制药有限公司 A kind of Chinese medicine composition for the treatment of tumor and preparation method thereof
CN105477045A (en) * 2016-01-19 2016-04-13 宁夏金太阳药业有限公司 Traditional Chinese medicine composition containing chrysanthemum and wild chrysanthemum volatile oil and preparation method of traditional Chinese medicine composition
CN105535439A (en) * 2016-01-19 2016-05-04 宁夏金太阳药业有限公司 Traditional Chinese medicinal composition for treating pharyngitis and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
刘雪梅等: "超临界CO2萃取桂郁金挥发油的化学成分", 《中国实验方剂学杂志》 *
高学忠等: "平消片为主组合方案治疗乳腺增生症临床分析", 《浙江中西医结合杂志》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110101823A (en) * 2019-06-19 2019-08-09 广州亿美化妆品生物科技有限公司 A kind of Chinese medical extract that treating the proliferation of mammary gland and its extracting method and application

Similar Documents

Publication Publication Date Title
CN102120020B (en) Medicinal composition for treating hyperplastic diseases of breast and hysteromyoma and preparation method thereof
CN101095923B (en) Chinese traditional medicine for treating ectopic pregnancy
CN105288041A (en) Traditional Chinese medicine preparation for preventing and treating nephrotic syndrome and preparation method thereof
CN106492069B (en) Chinese medicine composition and the application for preventing and treating chronic pelvic inflammatory disease
CN105816856A (en) Traditional Chinese medicine compound for preventing and treating livestock endometritis
CN102198242B (en) Traditional Chinese medicine composition for treating hysteromyoma
CN104510853A (en) Traditional Chinese medicinal effective part composition for treating dysmenorrhea of women and application of traditional Chinese medicinal effective part composition
CN105267906A (en) Compound chocolate Chinese herbal composition for treating and improving menstrual syndrome of females
CN103977362A (en) Traditional Chinese medicine composition for treating hyperplasia of mammary glands and early-stage breast cancer
CN104027684A (en) Traditional Chinese medicinal composition for treating gout and preparation method thereof
CN102048841B (en) Lactogenic traditional Chinese medicine composition and preparation method thereof
CN106110173A (en) A kind of Chinese medicine extract treating cyclomastopathy and extracting method thereof and application
CN101045152A (en) Traditional Chinese medicine composition for treating chronic pelvic inflammatory disease concomitant inflammatory swelling
CN101890062A (en) Use of nardostachys chinensis batal and extract thereof in preparation of medicaments for treating gastric ulcer
CN106421630A (en) Radix Curcumae Aromaticae extract product, and preparation method and application thereof
CN103495120B (en) A kind of Chinese medicine composition for the treatment of pelvic inflammatory disease and preparation method thereof
CN104324317A (en) Traditional Chinese medicine composition capable of treating uterine fibroids, and medicinal application
CN106039210A (en) Traditional Chinese medicinal composition for treating hyperplasia of mammary glands as well as preparation method and application thereof
CN105232746A (en) Traditional Chinese medicine composition and traditional Chinese medicine preparation for treating thoracic wall contusion and rib fracture and preparation method of traditional Chinese medicine preparation
CN105214048A (en) Be used for the treatment of the medicine of cold blood stasis type primary dysmenorrhea
CN106039209A (en) Traditional Chinese medicine extract for treating hyperplasia of mammary glands, as well as preparation method and application thereof
CN102920880B (en) External use traditional Chinese medicine for treating chronic hypertrophic rhinitis and preparation method thereof
CN110179893B (en) Enema liquid of ten ingredients with ginseng and angelica and preparation process thereof
CN105944104A (en) Pharmaceutical composition for treating digestive tract ulcer
CN106038802A (en) Preparation method of traditional Chinese medicinal composition for treating premature ovarian failure of women and traditional Chinese medicinal composition

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20161116

WD01 Invention patent application deemed withdrawn after publication