CN106039209A - Traditional Chinese medicine extract for treating hyperplasia of mammary glands, as well as preparation method and application thereof - Google Patents

Traditional Chinese medicine extract for treating hyperplasia of mammary glands, as well as preparation method and application thereof Download PDF

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CN106039209A
CN106039209A CN201610494719.4A CN201610494719A CN106039209A CN 106039209 A CN106039209 A CN 106039209A CN 201610494719 A CN201610494719 A CN 201610494719A CN 106039209 A CN106039209 A CN 106039209A
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radix curcumae
chinese medicine
extract
raffinate
extraction
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张军龙
濮存海
贾晓波
王伏军
马少平
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NINGXIA GOLD SUN PHARMACEUTICAL CO Ltd
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NINGXIA GOLD SUN PHARMACEUTICAL CO Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/906—Zingiberaceae (Ginger family)
    • A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/752—Citrus, e.g. lime, orange or lemon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

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Abstract

The invention discloses a traditional Chinese medicine extract for treating hyperplasia of mammary glands, as well as a preparation method and application thereof. The traditional Chinese medicine extract is prepared by the step of weighing 54 weight parts of radix curcumae, 54 weight parts of hairyvein agrimony and 90 weight parts of stir-baked fructus aurantii with bran in the following way (1) preparing a radix curcumae extract and a radix curcumae raffinate from radix curcumae by means of CO2 supercritical extraction; (2) performing mixing and alcohol extraction on the radix curcumae raffinate prepared from the step (1), stir-baked fructus aurantii with bran and hairyvein agrimony, filtering to obtain alcohol extracted medicinal residue and filtrate, and concentrating the filtrate to obtain alcohol extracted extract. The traditional Chinese medicine extract has the effects of soothing liver, regulating qi circulation, eliminating phlegm, removing stagnation, regulating qi, dredging channels, regulating chong and ren and the like, can be used for regulating the internal secretion and relieving pain, tumescence and other uncomfortable feelings, and has the effect of treating both symptoms and root cause of hyperplasia of mammary glands.

Description

A kind of Chinese medicine extract treating cyclomastopathy and its preparation method and application
Technical field
The present invention relates to technical field of Chinese medicines, in particular to being a kind of Chinese medicine extract treating cyclomastopathy and extraction thereof Methods and applications.
Background technology
The pathogenesis of cyclomastopathy: motherland's traditional medicine thinks that generation cyclomastopathy is appointed relevant with liver,spleen,kidney, punching, many Having mechanism of qi obstructed, blood stasis, expectorant are solidifying, make the strongly fragrant resistance of meridian qi and blood be agglomerated into caused by core.Ancients think " disease of married woman arises from strongly fragrant more ", Owing to women easily suffers from stagnation of liver-QI for the psychological characteristics of feelings will change.Meanwhile, motherland's medical science thinks that gas and blood are closely bound up, gas Handsome for blood, gas row then blood, the stagnation of QI then blood stasis, hematogenous blockage then mammary gland changes therewith, thus easily forms liver energy stagnation type breast Gland hypertrophy is sick.
The important pathogenesis of cyclomastopathy is stagnation of QI due to depression of the liver liver controlling conveyance and dispersion, and irritability is the happiest and bar reaches, liver being bold and firm viscera, and body the moon is used Sun preferably rises and sends out and evacuate.Irritability had both disliked depression, also avoided high.Owing to feelings will is unsuccessful, melancholy puzzled, long strongly fragrant impairing the liver, or by essence God stimulates, irritable angry, all may result in depression of liver-QI, and the mechanism of qi stasis of blood is stagnant, pents up in breast stomach network, and breast network meridian and vessels obstruction is obstructed, no General rule causes mastalgia bitterly.
The treatment of cyclomastopathy can use blood circulation promoting and blood stasis dispelling and dissipating phlegm and resolving masses method, and activating blood and removing stasis Method is the conventional side for the treatment of nodules of the breast Method.The traditional Chinese medical science has saying of " pathogen usually intruding into collateral in protracted disease ", and nodules of the breast onset is slow, and the course of disease is long, touching does not heals, therefore lump in breast is to tie in breast for accumulation of phlegm in the hypochondrium Caused by network.Qi depression to blood stasis, breast network blocks, is the basic pathogenesis of cyclomastopathy." addiction " " knot " " bitterly " is the sick total clinic of breast Feature, no matter the breast of which kind of pattern of syndrome is sick, on the basis of differentiation of symptoms and signs for classification of syndrome treats, all needs the method for R. concomitans blood circulation promoting and blood stasis dispelling to dredge gas Blood, makes addiction blood agglomeration blockization dissipate, and breast network is unobstructed and swells and ache from disappearing.Modern medicine study result also confirms that invigorating blood circulation addiction method can change Being apt to or correct blood circulation and microcirculation disturbance, drug for invigorating blood circulation and eliminating stasis has reduction blood viscosity, accelerates the effects such as blood flowing, Thus promote the lump of hypertrophy and fiber to absorb.
At present, the treatment to cyclomastopathy is not had to have the product of remarkable result；Existing procucts are general not by its effective ingredient Extract largely, also or heavy metal and harmful element content the highest.
Summary of the invention
Present invention aim to provide the Chinese medicine extract of a kind of effective treatment cyclomastopathy.This Chinese medicine extract rises To effect conditioning endocrine such as depressed liver-energy dispersing and QI regulating, dissipating phlegm and resolving masses, regulating qi and dredging collateral, Chong and Ren Meridians regulating, fundamentally disintegrate cyclomastopathy Primary factors, adds that TAM, to estrogen antagonist, blocks its effect making gland tissue hyperplasia and edema, releases pain and swollen Completely wait discomfort, effect for the treatment of both the principal and secondary aspects of a disease can be reached.
It is a further object of the present invention to provide the Chinese medicine extract preparation method of this treatment cyclomastopathy.
It is an object of the invention to be accomplished by:
A kind of Chinese medicine extract treating cyclomastopathy, is prepared by the following method and obtains:
Take Radix Curcumae 54 weight portion, Herba Agrimoniae 54 weight portion, stir-baked Fructus Aurantii in bran 90 weight portion；
Above-mentioned raw materials is prepared in such a way:
(1) Radix Curcumae is through CO2Supercritical extraction obtains Radix Curcumae extract and Radix Curcumae raffinate；
(2) Radix Curcumae raffinate step (1) obtained and stir-baked Fructus Aurantii in bran and Herba Agrimoniae mixing alcohol extraction, filters, obtains alcohol extraction Medicinal residues and filtrate, filtrate concentrates, obtains alcohol-extracted extract.
CO described in step (1)2Supercritical extraction condition is: extracting pressure is 25-35MPa, extraction temperature 25-35 DEG C, CO2Flow 11-15L/h, extraction time 1-2h, take Radix Curcumae raffinate.
CO described in preferred steps (1)2Supercritical extraction condition is: extracting pressure is 35MPa, extraction temperature 30 DEG C, CO2 Flow 13L/h, extraction time 1.5h, take Radix Curcumae raffinate.Separating still I pressure 10～11MPa, temperature 48 DEG C；Separating still II is pressed Power 7～8MPa, temperature 36 DEG C.
Described step (2) may comprise steps of: takes the stir-baked Fructus Aurantii in bran of recipe quantity, Herba Agrimoniae and Radix Curcumae raffinate Adding 6-10 times of weight 70%-90% alcohol reflux of three kinds of mixture 1-3 time, each 1-2 hour, backflow stood 12 hours Above, Aspirate supernatant, 300 mesh filter, and filtrate is evaporated to the alcohol extraction of relative density 1.15～1.25 under the conditions of 60 DEG C Extractum.
Described step (2) specifically includes following steps: takes the stir-baked Fructus Aurantii in bran of recipe quantity, Herba Agrimoniae and Radix Curcumae raffinate and adds 8 times of weight 80% alcohol reflux of three kinds of mixture twice, each 2 hours, backflow stood more than 12 hours, drew supernatant Liquid, filters, the clear paste of relative density 1.15～1.25 under the conditions of filtrate reduced in volume to 60 DEG C.
Dosage form made by above-mentioned Chinese medicine extract and pharmaceutically acceptable adjuvant, and preferred dosage form is tablet, capsule, granule Agent, pill.
The preparation method of the Chinese medicine extract of above-mentioned treatment cyclomastopathy, the method comprises the following steps:
Take Radix Curcumae 54 weight portion, Herba Agrimoniae 54 weight portion, stir-baked Fructus Aurantii in bran 90 weight portion；
Above-mentioned raw materials is prepared in such a way:
(1) Radix Curcumae is through CO2Supercritical extraction obtains Radix Curcumae extract and Radix Curcumae raffinate；
(2) Radix Curcumae raffinate step (1) obtained and stir-baked Fructus Aurantii in bran and Herba Agrimoniae mixing alcohol extraction, filters, obtains alcohol extraction Medicinal residues and filtrate, filtrate concentrates, obtains alcohol-extracted extract.
The Chinese medicine extract for the treatment of cyclomastopathy of the present invention is in preparation treatment or prevention or assists treatment mammary gland to increase The raw application in medicine.
Divide in effect conditionings such as Chinese medicine extract function depressed liver-energy dispersing and QI regulating of the present invention, dissipating phlegm and resolving masses, regulating qi and dredging collateral, Chong and Ren Meridians regulating Secrete.
In this prescription, the pharmacology of each medicine is:
Radix Curcumae is zingiberaceous plant RADIX CURCUMAE (Cur cuma weny uj in Y.H.Chenet C.Ling), Rhizoma Curcumae Longae (Curclma longa L.), Guangxi zedoary (Curcumakw angsiensis S.G.Lee et C.F.Liang) or fluffy cowherb The dried root of art (Curcuma haeocaulis Val.).The above two practise respectively title " RADIX CURCUMAE " with " Radix Curcuma ", remaining Practise by character difference and claim osmanthus Radix Curcumae or green filament Radix Curcumae.Radix Curcumae mainly originates in the ground such as Sichuan, Fujian, Zhejiang, Guangxi.In Radix Curcumae mainly Containing compositions such as volatile oil, curcumin, polysaccharide, a small amount of trace element, wherein volatile oil is Radix Curcumae antineoplastic effective composition, Rhizoma Curcumae Longae Element is the principle active component of Rhizoma Curcumae blood fat reducing, antioxidation, antiinflammatory.Modern pharmacology research shows, it is thin that Radix Curcumae has protection liver Born of the same parents, promotion liver cell regeneration, anticancer, antibacterial, antioxidative effect.Volatile oil and curcumin are the principle active component of Radix Curcumae. Radix Curcumae volatile oil not only has the effect of sterilization, antiviral, antiinflammatory and blood circulation promoting and blood stasis dispelling, removing the necrotic tissue and promoting granulation, and may also suppress tumor The growth of cell, has positive role to prevention cervical cancer.Curcumin has the multiple pharmacology such as antitumor, antiinflammatory, antibacterial, antioxidation Effect, and toxicity is low, has good clinical practice potentiality, is paid close attention to widely by both at home and abroad.
Fructus Aurantii is rutaceae Citrus aurantium Linn. (Citrus aurantium L1) and the dry crude fruit of variety thereof Real.Bitter in the mouth, pungent, sour.Cold nature.Return spleen, stomach warp.There is regulating the flow of QI to ease the stomach, effect of the stagnant relieving distension of row.The stagnation of QI, distension is coerced for breast Pain, accumulation of food in the stomach and intes tine due to indigestion, stops in phlegm retention, visceroptosis.Fructus Aurantii is one of typical QI regulating medicine of Chinese medicine, is widely used in TCM Bed.Contained by Fructus Aurantii, chemical composition is complex, the effective ingredient being currently known mainly have flavonoid, volatile oil, alkaloids, Coumarins and some trace element etc..From the point of view of PINGXIAO PIAN is used for treating cyclomastopathy, we mainly take Fructus Aurantii Hesperidin, naringin and Nobiletin in flavones ingredient.Hesperidin has antiinflammatory action, antitumaous effect.Naringin has Anti-inflammatory and analgesic effect.Nobiletin has the pharmacological actions such as antiinflammatory, antiviral, antioxidation.
Herba Agrimoniae is the ground drying nest of rosaceous plant Radix Agrimoniae (Agrimonia Pilosa Ledeb), mainly becomes Dividing and have flavonoid, agrimonine, agrimonolide, tannin, volatile oil, organic acid and agrimophol etc., property is bitter, puckery, GUIXIN, Liver Channel, has astringing to arrest bleeding, preventing the attack (or recurrence) of malaria, dysentery relieving, Detoxication, can be used for treat hemoptysis, haematemesis, metrostaxis, malaria, dysentery, De-power impairment caused by overstrain, carbuncle sore tumefacting virus, pudendal pruritus leukorrhagia.
Chinese medicine extract of the present invention has the merits such as blood circulation promoting and blood stasis dispelling, pain relieving eliminating stagnation, heat-clearing and toxic substances removing, strengthening vital QI to eliminate pathogenic factors, qi-restoratives dredging collateral Effect, the tuberosity for each phase hypertrophy all has good dissipation role.Utilize extract depressed liver-energy dispersing and QI regulating of the present invention, dissipating phlegm and resolving masses, reason Effect conditioning endocrine such as gas dredging collateral, Chong and Ren Meridians regulating, fundamentally disintegrate the Primary factors of cyclomastopathy, add that TAM antagonism is female Hormone, blocks its effect making gland tissue hyperplasia and edema, releases the discomfort such as pain and distension, to reach treating both the principal and secondary aspects of a disease Effect.Additionally, also cyclomastopathy tuberosity there to be good eliminating stagnation effect.
In this technique, (1) uses CO2Volatile oil in Radix Curcumae is preferentially proposed by supercritical technology, obtains volatile oil and raffinate； (2) Radix Curcumae raffinate, Herba Agrimoniae, stir-baked Fructus Aurantii in bran use alcohol extraction according to its effective ingredient character, obtain alcohol-extracted extract.The present invention has Advantages below: (1) uses the method for alcohol extraction, the liposoluble constituent such as Polyphenols in medical material, flavonoid extracts relatively complete, and (2) will The alcohol extraction together with stir-baked Fructus Aurantii in bran etc. of Radix Curcumae raffinate makes medical material at utmost to be utilized.The present invention treats cyclomastopathy Chinese medicine extract can make various dosage form (hard capsule that includes taking orally, soft capsule, tablet, granule, drop pill).
The know-why of the present invention and technical study:
One, the determination of alcohol extraction process parameter
1, orthogonal test affects the principal element of alcohol extraction process concentration of alcohol, solvent times amount, return time and extraction time Number.Above-mentioned 4 factors respectively take 3 levels and carry out L9(34) orthogonal test, take the Radix Curcumae CO of recipe quantity2The medicinal residues of supercritical extraction, The factor level table of stir-baked Fructus Aurantii in bran and Herba Agrimoniae according to the form below extracts, and screens optimum extraction process, and experimental factor level is shown in Table 1.
The quadrature factor water-glass of table 1 alcohol extraction condition
The present invention selects dry spun (%), general flavone content (%) to be inspection target, orthogonal experiment is carried out data and divides Analysis.Orthogonal calendar and the results are shown in Table 2.
Table 2 dry spun (%) and orthogonal arrangement that general flavone content (%) is inspection target and result table
(table provides 9 experimental programs.If No. 6 experiment is A2B3C1D2, i.e. 80% ethanol, 10 times amount reflux, extract, 2 times, each 1h, the rest may be inferred)
With general flavone content as index, range analysis result it is A > C > D > B, the best of breed between each factor and level For A3B2C3D2, with B item as error term, carry out variance analysis further, the results are shown in Table 3.
The table 3 analysis of variance table with general flavone content as index
(F0.05(2,2)=19.00), with general flavone content as index, there are no significant to experimental result for each factor level Impact, extraction process best of breed is A3B2C3D2。
With dry spun as index, range analysis result is A > D > C > B, and the best of breed between each factor and level is A2B3C2D3, with B item as error term, carry out variance analysis further, the results are shown in Table 4.
The table 4 analysis of variance table with dry spun as index
(F0.05(2,2)=19.00), with dry spun as index, each factor on experimental result there are no significant impact, carry Taking technique best of breed is A2B3C2D3。
For the orthogonal result of more rational evaluation, find optimum extraction process, two evaluation indexes are carried out comprehensive grading, calculate Method is as follows: in two indices, chooses soprano respectively as 100 points, and remaining is pressed formula and calculates: score Y=weight × (100-peak+yi), to two indices weight coefficient respectively for for 0.6 and 0.4.Analyzed by directly perceived, with comprehensive grading For index, showing that each factor is A > C > D > B on extracting result impact order, the best of breed between each factor and level is A2B2C3D2, with B item as error term, carry out variance analysis further, the results are shown in Table 5.
The table 5 analysis of variance table with comprehensive grading as index
(F0.05(2,2)=19.00) with comprehensive grading index as index, each factor to experimental result not statistically significant, Extraction process best of breed is A2B2C3D2。
2, alcohol extraction confirmatory experiment
In order to verify the above results, take above-mentioned three kinds of medical materials of 0.1 times of recipe quantity, select A2B2C3D2Condition is verified Experiment, obtains result such as table 6；
Table 6 alcohol extraction confirmatory experiment result table
Experiment number General flavone content (%) Dry spun (%)
1 35.69 29.68
2 34.78 31.76
3 35.33 30.45
The result of confirmatory experiment is consistent substantially with Orthogonal Parameter, so the optimum process condition of alcohol extraction experiment is concentration of alcohol Being 80%, ethanol consumption is 8 times amount, extracts twice, each 2h.
We carry out Experiment on therapy to the raw material components side of tearing open of the present invention, effect of the most each prescription, three prescriptions It is respectively as follows:
Prescription 1: of the present invention group of (alcohol-extracted extract group) Radix Curcumae 54 weight portion is through CO2Supercritical extraction, obtains raffinate；Raffinate Mix homogeneously with 54 weight portion Herba Agrimoniaes and 90 parts of stir-baked Fructus Aurantii in bran, add the ethanol extraction twice of 8 times amount 80%, each 2h, return Flow liquid stands more than 12 hours, Aspirate supernatant, filters, and during filtrate reduced in volume to 60 DEG C, relative density 1.15～1.25 is clear Cream, obtains alcohol-extracted extract.
Prescription 2: Herba Agrimoniae extractum group (alcohol extraction) 54 weight portion Herba Agrimoniae adds the ethanol extraction twice of 8 times amount 80%, often Secondary 2h, backflow stand more than 12 hours, Aspirate supernatant, filter, during filtrate reduced in volume to 60 DEG C relative density 1.15～ The clear paste of 1.25, obtains Herba Agrimoniae extractum group.
Prescription 3: stir-baked Fructus Aurantii in bran extractum group (alcohol extraction) 90 weight portion stir-baked Fructus Aurantii in bran adds the ethanol extraction two of 8 times amount 80% Secondary, each 2h, backflow stands more than 12 hours, and Aspirate supernatant filters, relative density during filtrate reduced in volume to 60 DEG C The clear paste of 1.15～1.25, obtains stir-baked Fructus Aurantii in bran extractum group.
1, female non-pregnant rat about the 220g of laboratory animal: SD 80
2, reagent: estradiol benzoate injection specification 2g.L-1/, Shanghai General Pharmaceutical Co., ltd.'s lot number 150501
Estradiol (E2), progesterone (P) test kit all has English biotechnology research institute of Beijing China product batch number 151012
Slide gauge Shenzhen Xin Baolai detecting instrument company limited
Refrigerated centrifuger Beijing epoch Bei Li centrifuge company limited
R-911 Full automatic exempts from industry head office of calculating instrument Chinese University of Science and Technology
3, method
3.1 take 50 SD rats is randomly divided into 5 groups, model group (normal saline 15ml/), blank group (etc. Amount normal saline 15ml/), prescription 1 (2.4g/), prescription 2 (2.4g/), prescription 3 (2.4g/).Blank group is big Mus intramuscular injection normal saline (0.05ml/ only), once a day, each model group rats intramuscular injection estradiol benzoate 0.5/ , extremely experiment terminates once a day.Modeling started gastric infusion after 1 week, every day 1 time, was administered 4 weeks, and last is drawn materials after being administered 2h.
The forward and backward slide gauge of mensuration modeling of 3.2 cyclomastopathy accurately measures rat the 2nd to 2, left and right breast diameter And height of nipples, and observe its outward appearance.
Fasting 12h before 3.3 hormone determination experiments, after last is administered 2h, abdominal aortic blood 5mL, 3000r min-1High Speed frozen centrifugation 15min, takes serum and adds the cold petroleum ether of 4mL, recentrifuge after mixing.Phase is abandoned in suction, after lower nitrogen dries up It is dried and seals, to be measured.
Use r-911 Full automatic to exempt from calculating instrument and radioimmunoassay kits, by product description, serum is carried out female Glycol (E2), progesterone (P) detection.
4 results
4.1 on papilla diameter, height of nipples impact
Compared with normal group, model group rats papilla diameter and highly significant increase, and nipple holds up hyperemia.In injection female two Alcohol reaches swelling peak on the 2nd week, and within the 3rd week, swelling maintains an equal level, and begins to decline at the 4th week.Prescription 1, prescription 2,3 groups of nipples of prescription are straight Footpath, height are compared with model group and to be substantially reduced.Result shows, the cyclomastopathy that estradiol is induced is had and significantly presses down by this extract Make and use.
The change of table 7 rat papilla diameter (N=10)
Compare with blank groupbP<0.001；Compare with model control groupcP<0.001；dP<0.05；Compare with prescription 1eP<0.05；fP<0.01；
The change of table 8 rat height of nipples (N=10)
Compare with blank groupbP<0.001；Compare with model control groupcP<0.001；dP<0.05；Compare with prescription 1eP<0.05；
4.2 couples of serum estradiol (E2), the impact of progesterone (P) content
Testing result shows, positive controls and PINGXIAO PIAN high dose group relatively model group estrogen level decrease, pregnant Ketone level raises, and result see table
Table 9 rat is to serum estradiol (E2), the change of progesterone (P) content (N=10)
Compare with blank groupbP<0.001；Compare with model control groupcP<0.001；dP<0.01；eP<0.05；
Compare with prescription 1fP<0.05；
5. discuss
Being illustrated by above results of animal, extract of the present invention has the effect for the treatment of cyclomastopathy, improves The quality of life of patient, and any toxic and side effects and untoward reaction do not occur in experimentation.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1:
Radix Curcumae 54g, Herba Agrimoniae 54g, stir-baked Fructus Aurantii in bran 90g；
Its preparation method is:
(1) Radix Curcumae is taken through CO2Supercritical extraction obtains extract and raffinate.Extracting pressure is 35MPa, extraction temperature 30 DEG C, CO2Flow 13L/h, extraction time 1.5h, take Radix Curcumae raffinate.Separating still I pressure 10～11MPa, temperature 48 DEG C；Separate Still II pressure 7～8MPa, temperature 36 DEG C.
(2) take the raffinate in (1), Herba Agrimoniae and stir-baked Fructus Aurantii in bran and add 10 times amount 90% alcohol reflux 2 times, each 2 Hour, backflow stands more than 12 hours, Aspirate supernatant, and 300 mesh filter, and filtrate is evaporated under the conditions of 60 DEG C relatively The alcohol-extracted extract of density 1.15～1.25, standby；General flavone content is 35.12%, and dry spun is 30.86%.
Embodiment 2:
Radix Curcumae 54g, Herba Agrimoniae 54g, stir-baked Fructus Aurantii in bran 90g；
Its preparation method is:
(1) Radix Curcumae is taken through CO2Supercritical extraction obtains extract and raffinate.Extracting pressure is 35MPa, extraction temperature 30 DEG C, CO2Flow 13L/h, extraction time 1.5h, take Radix Curcumae raffinate.Separating still I pressure 10～11MPa, temperature 48 DEG C；Separate Still II pressure 7～8MPa, temperature 36 DEG C.
(2) take the raffinate in (1), Herba Agrimoniae and stir-baked Fructus Aurantii in bran and add 8 times amount 80% alcohol reflux 3 times, each 2 little Time, backflow stands more than 12 hours, Aspirate supernatant, and 300 mesh filter, and filtrate is evaporated to the closeest under the conditions of 60 DEG C The alcohol-extracted extract of degree 1.15～1.25, standby；General flavone content is 34.52%, and dry spun is 29.76%.
Embodiment 3:
Radix Curcumae 54g, Herba Agrimoniae 54g, stir-baked Fructus Aurantii in bran 90g；
Its preparation method is:
(1) Radix Curcumae is taken through CO2Supercritical extraction obtains extract and raffinate.Extracting pressure is 35MPa, extraction temperature 30 DEG C, CO2Flow 13L/h, extraction time 1.5h, take Radix Curcumae raffinate.Separating still I pressure 10～11MPa, temperature 48 DEG C；Separate Still II pressure 7～8MPa, temperature 36 DEG C.
(2) take the raffinate in (1), Herba Agrimoniae and stir-baked Fructus Aurantii in bran and add 8 times amount 80% alcohol reflux 2 times, each 1.5 Hour, backflow stands more than 12 hours, Aspirate supernatant, and 300 mesh filter, and filtrate is evaporated under the conditions of 60 DEG C relatively The alcohol-extracted extract of density 1.15～1.25, standby；General flavone content is 35.61%, and dry spun is 30.23%.
Embodiment 4:
Radix Curcumae 54g, Herba Agrimoniae 54g, stir-baked Fructus Aurantii in bran 90g；
Its preparation method is:
(1) Radix Curcumae is taken through CO2Supercritical extraction obtains extract and raffinate.Extracting pressure is 35MPa, extraction temperature 30 DEG C, CO2Flow 13L/h, extraction time 1.5h, take Radix Curcumae raffinate.Separating still I pressure 10～11MPa, temperature 48 DEG C；Separate Still II pressure 7～8MPa, temperature 36 DEG C.
(2) take the raffinate in (1), Herba Agrimoniae and stir-baked Fructus Aurantii in bran and add 10 times amount 70% alcohol reflux 3 times, each 2 Hour, backflow stands more than 12 hours, Aspirate supernatant, and 300 mesh filter, and filtrate is evaporated under the conditions of 60 DEG C relatively The alcohol-extracted extract of density 1.15～1.25, standby；General flavone content is 33.93%, and dry spun is 30.36%.
Embodiment 5
The broken rear adjuvants such as starch that add of dried cream powder prepared according to embodiment 1 method prepare according to common process Granule.
Embodiment 6
The broken rear adjuvants such as starch that add of dried cream powder prepared according to embodiment 1 method prepare according to common process Granule, then tabletting prepares tablet.
Embodiment 7
The broken rear adjuvants such as starch that add of dried cream powder prepared according to embodiment 1 method prepare according to common process Granule, then fill gelatine capsule leather obtains capsule.
Embodiment 8
The broken rear adjuvant that adds of dried cream powder prepared according to embodiment 1 method prepares honeyed pill according to common process.
Embodiment 9
The broken rear adjuvant that adds of dried cream powder prepared according to embodiment 1 method prepares drop pill according to common process.

Claims (8)

1. the Chinese medicine extract treating cyclomastopathy, it is characterised in that the Chinese medicine extract of this treatment cyclomastopathy is to pass through Following methods prepares:
Take Radix Curcumae 54 weight portion, Herba Agrimoniae 54 weight portion, stir-baked Fructus Aurantii in bran 90 weight portion；
Above-mentioned raw materials is prepared in such a way:
(1) Radix Curcumae is through CO2Supercritical extraction obtains Radix Curcumae extract and Radix Curcumae raffinate；
(2) Radix Curcumae raffinate step (1) obtained and stir-baked Fructus Aurantii in bran and Herba Agrimoniae mixing alcohol extraction, filters, obtains alcohol extraction medicinal residues And filtrate, filtrate concentrates, obtains alcohol-extracted extract.
The Chinese medicine extract for the treatment of cyclomastopathy the most according to claim 1, it is characterised in that the CO described in step (1)2Super Critical extraction conditions is: extracting pressure is 25-35MPa, extraction temperature 25-35 DEG C, CO2Flow 11-15L/h, extraction time 1- 2h, takes Radix Curcumae raffinate.
The Chinese medicine extract for the treatment of cyclomastopathy the most according to claim 2, it is characterised in that the CO described in step (1)2Super Critical extraction conditions is: extracting pressure is 35MPa, extraction temperature 30 DEG C, CO2Flow 13L/h, extraction time 1.5h, take Radix Curcumae Raffinate.
The Chinese medicine extract for the treatment of cyclomastopathy the most according to claim 1, it is characterised in that described step (2) is concrete Comprise the following steps: take the stir-baked Fructus Aurantii in bran of recipe quantity, Herba Agrimoniae and Radix Curcumae raffinate and add 6-10 times of weight of three kinds of mixture 70%-90% alcohol reflux 1-3 time, each 1-2 hour, backflow stood more than 12 hours, Aspirate supernatant, 300 mesh Filtering, filtrate is evaporated to the alcohol-extracted extract of relative density 1.15～1.25 under the conditions of 60 DEG C.
The Chinese medicine extract for the treatment of cyclomastopathy the most according to claim 1, it is characterised in that described step (2) is concrete Comprise the following steps: take the stir-baked Fructus Aurantii in bran of recipe quantity, Herba Agrimoniae and Radix Curcumae raffinate and add 8 times of weight 80% ethanol of three kinds of mixture Reflux, extract, twice, each 2 hours, backflow stood more than 12 hours, Aspirate supernatant, filtered, filtrate reduced in volume to 60 The clear paste of relative density 1.15～1.25 under the conditions of DEG C.
The Chinese medicine extract for the treatment of cyclomastopathy the most according to claim 1, it is characterised in that described Chinese medicine extract Making dosage form with pharmaceutically acceptable adjuvant, preferred dosage form is tablet, capsule, granule, pill.
7. the preparation method of the Chinese medicine extract of the treatment cyclomastopathy described in a claim 1, it is characterised in that the method Comprise the following steps:
Take Radix Curcumae 54 weight portion, Herba Agrimoniae 54 weight portion, stir-baked Fructus Aurantii in bran 90 weight portion；
Above-mentioned raw materials is prepared in such a way:
(1) Radix Curcumae is through CO2Supercritical extraction obtains Radix Curcumae extract and Radix Curcumae raffinate；
(2) Radix Curcumae raffinate step (1) obtained and stir-baked Fructus Aurantii in bran and Herba Agrimoniae mixing alcohol extraction, filters, and filtrate concentrates, and obtains Alcohol-extracted extract.
8. the Chinese medicine extract of the treatment cyclomastopathy described in claim 1 in preparation treatment or prevention or assists treatment mammary gland to increase The raw application in medicine.
CN201610494719.4A 2016-06-29 2016-06-29 Traditional Chinese medicine extract for treating hyperplasia of mammary glands, as well as preparation method and application thereof Pending CN106039209A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101461926A (en) * 2009-01-15 2009-06-24 郁慧 Medicament composition for treating gland hyperplasia and preparation method thereof
CN101612357A (en) * 2009-02-13 2009-12-30 西安正大制药有限公司 A kind of Chinese medicine composition for the treatment of tumor and preparation method thereof
CN101574506B (en) * 2008-05-29 2010-12-29 宁夏金太阳药业有限公司 Method for preparing pingxiao preparation
CN105477045A (en) * 2016-01-19 2016-04-13 宁夏金太阳药业有限公司 Traditional Chinese medicine composition containing chrysanthemum and wild chrysanthemum volatile oil and preparation method of traditional Chinese medicine composition
CN105535439A (en) * 2016-01-19 2016-05-04 宁夏金太阳药业有限公司 Traditional Chinese medicinal composition for treating pharyngitis and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101574506B (en) * 2008-05-29 2010-12-29 宁夏金太阳药业有限公司 Method for preparing pingxiao preparation
CN101461926A (en) * 2009-01-15 2009-06-24 郁慧 Medicament composition for treating gland hyperplasia and preparation method thereof
CN101612357A (en) * 2009-02-13 2009-12-30 西安正大制药有限公司 A kind of Chinese medicine composition for the treatment of tumor and preparation method thereof
CN105477045A (en) * 2016-01-19 2016-04-13 宁夏金太阳药业有限公司 Traditional Chinese medicine composition containing chrysanthemum and wild chrysanthemum volatile oil and preparation method of traditional Chinese medicine composition
CN105535439A (en) * 2016-01-19 2016-05-04 宁夏金太阳药业有限公司 Traditional Chinese medicinal composition for treating pharyngitis and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
刘雪梅等: "超临界CO2萃取桂郁金挥发油的化学成分", 《中国实验方剂学杂志》 *
高学忠等: "平消片为主组合方案治疗乳腺增生症临床分析", 《浙江中西医结合杂志》 *

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