CN106106150B - 通过根尖培育良种玄参的方法 - Google Patents
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Abstract
本发明公开了一种通过根尖培育良种玄参的方法，涉及中药材人工种植技术领域。茎尖和根尖的分生组织中一般是无毒或仅含极低浓度的病毒粒子，利用细胞的全能性，通过茎尖或者根尖分裂分化可产生完整的、无病毒的植株，但是，通过根尖分裂分化培育植株的方法几乎没有文献记载。本发明提出了根尖培养的方式，获得优良无病毒的玄参幼苗，将其进行种植，解决了玄参由于长期无性繁殖引起的病毒累计严重的问题，并增加了玄参的产量、保持了野生玄参的所有优良特性及有效中药成分含量。
Description
技术领域
本发明涉及中药材人工种植技术领域，尤其是一种通过根尖培育良种玄参的方法。
背景技术
玄参，又叫元参、浙玄参、黑参，为玄参科植物，拉丁名为FigwortRoot。主治:温热病热和营血、身热、烦渴、舌绛、发斑、骨蒸劳嗽、虚烦不寤、津伤便秘、目涩昏花、咽喉喉肿痛、瘰疬痰核、痈疽疮毒。由于人们对该类药物的需求不断增长，而野生资源又不足，因此，采取人工种植的方法培育加工玄参是一种有效途径。
人工栽培玄参有利用种子进行有性繁殖方式和利用植物细胞的全能性无性繁殖方式，无性繁殖方式能保存优质品种和缩短栽培时间，但是由于缺乏品种的提纯复壮，多年来无性繁殖下来病毒累积严重，使得玄参的病虫害发生率上升，产量下降。所以需要对玄参进行无害化处理，培育出良种玄参进行栽培。茎尖和根尖的分生组织中一般是无毒或仅含极低浓度的病毒粒子，利用细胞的全能性，通过茎尖或者根尖分裂分化可产生完整的、无病毒的植株。发明专利申请CN105309311A（申请号是201510747917.2）公开了一种通过茎尖培育的玄参良种繁育方法，对于通过根尖分裂分化培育的方法则没有提及。同时，通过根尖分裂分化培育植株的方法几乎没有文献记载。
发明内容
本发明提供一种通过根尖培育良种玄参的方法，它可以解决玄参由于长期无性繁殖引起的病毒累计严重的问题。
为了解决上述问题，本发明所采用的技术方案是：这种通过根尖培育良种玄参的方法，包括如下步骤：
A、植株筛选：在玄参成熟时或者萌芽时，选择无病虫害的植株；
B、预处理和消毒：取步骤A所得植株的根尖1～2cm，用清水清洗干净，再用70%的酒精漂洗30s，然后用0.1%的HgCl2溶液灭菌3min，无菌水洗3遍及以上；
C、将步骤B所得的根尖置于显微镜下，用酒精浸泡后，再用灼烧冷却的镊子和剪刀将根尖分生区细胞切成3～7个根尖小段，再用无菌水清洗3遍及以上；
D、培育：将步骤C所得根尖小段放入诱导培养基中进行培养15～25天，所述诱导培养基是MS+6-苄基嘌呤1.5mg/L +萘乙酸1.0mg/L，得到预生根，将所述预生根的根尖1～2cm切成3～7个根尖小段接入所述诱导培养基培养15～25天，重复上述步骤至得到适当数量的预生根后，将所述预生根25～30个为一批，分批接入增殖培养基，所述增殖培养基是MS+6-苄基嘌呤1.5mg/L+萘乙酸1.5mg/L，培养至长出3～4 cm丛生芽；
E、将步骤D所得长叶长根的幼苗放入阳光下3～5天，取出幼苗，将其移栽至育苗床，行距5～6cm、株距5～6cm，开深3～4cm，每处栽种一株幼苗，保持育苗床湿润；所述育苗床是由甘蔗叶作为基质，混合有蔗糖厂滤泥和/或者鱼塘淤泥、磷酸氢二钾、花生麸、腐殖质、硫酸锰、硫酸锌发酵而得；当幼苗长出6～7片复叶，60%植株出现五出复叶时，将其移栽至大田，即可。
上述技术方案中，更具体的技术方案还可以是：所述培育阶段的培养条件为温度23～28℃，光照强度1500～1800LUX。
进一步的：所述育苗床的各成分比重为甘蔗叶60～75重量份、蔗糖厂滤泥和/或者鱼塘淤泥20～25重量份、磷酸氢二钾5～10重量份、腐殖质5～10重量份、硫酸锰1～5重量份、硫酸锌1～5重量份、花生麸1～2重量份。
由于采用了上述技术方案，本发明与现有技术相比具有如下有益效果：
1、本发明提出了根尖培养的方式，获得优良无病毒的玄参幼苗，将其进行种植，植株生长健壮，长势一致，病虫害发生率只有8%左右，远远低于普通无性繁殖35%左右的病虫害发生率。
2、用本发明培育的良种幼苗进行栽种，玄参的产量能增加30%左右，并能保持野生玄参的所有优良特性及有效中药成分含量。
具体实施方式
以下结合具体实施例对本发明作进一步详述：
这种通过根尖培育玄参良种的方法如下：
A、植株筛选：在在9月底至11月上旬玄参成熟时或者3月份萌芽时，选择无病虫害的植株；
B、预处理和消毒：取步骤A所得植株的根尖1～2cm，用清水清洗干净，再用70%的酒精漂洗30s，然后用0.1%的HgCl2溶液灭菌3min，无菌水洗3遍；
C、将步骤B所得的根尖置于显微镜下，用酒精浸泡后，再用灼烧冷却的镊子和剪刀将根尖分生区细胞切成5个根尖小段，再用无菌水清洗3遍；
D、培育：将步骤C所得根尖小段放入诱导培养基中进行培养15天，所述诱导培养基是MS+6-苄基嘌呤1.5mg/L +萘乙酸1.0mg/L，得到预生根，将所述预生根的根尖1～2cm切成5个根尖小段接入所述诱导培养基培养15～25天，重复上述步骤至得到适当数量的预生根后，将所述预生根30个为一批，分批接入增殖培养基，所述增殖培养基是MS+6-苄基嘌呤1.5mg/L+萘乙酸1.5mg/L，培养至长出3～4 cm丛生芽；
以上培养条件为温度23～28℃，光照强度1500～1800LUX；
E、将步骤D所得长叶长根的幼苗放入阳光下3～5天，取出幼苗，将其移栽至育苗床，行距5～6cm、株距5～6cm，开深3～4cm，每处栽种一株幼苗，保持育苗床湿润；所述育苗床是由甘蔗叶作为基质，混合有滤泥、磷酸氢二钾、腐殖质、硫酸锰、硫酸锌、花生麸发酵而得；其中甘蔗叶60～75重量份、鱼塘淤泥20～25重量份、磷酸氢二钾5～10重量份、腐殖质5～10重量份、硫酸锰1～5重量份、硫酸锌1～5重量份、花生麸1～2重量份；当幼苗长出6～7片复叶，60%植株出现五出复叶时，将其移栽至大田，即可。
Claims (3)
1.一种通过根尖培育良种玄参的方法，其特征在于包括如下步骤：
A、植株筛选：在玄参成熟时或者萌芽时，选择无病虫害的植株；
B、预处理和消毒：取步骤A所得植株的根尖1～2cm，用清水清洗干净，再用70%的酒精漂洗30s，然后用0.1%的HgCl2溶液灭菌3min，无菌水洗3遍及以上；
C、将步骤B所得的根尖置于显微镜下，用酒精浸泡后，再用灼烧冷却的镊子和剪刀将根尖分生区细胞切成3～7个根尖小段，再用无菌水清洗3遍及以上；
D、培育：将步骤C所得根尖小段放入诱导培养基中进行培养15～25天，所述诱导培养基是MS+6-苄基嘌呤1.5mg/L +萘乙酸1.0mg/L，得到预生根，将所述预生根的根尖1～2cm切成3～7个根尖小段接入所述诱导培养基培养15～25天，重复上述步骤至得到适当数量的预生根后，将所述预生根25～30个为一批，分批接入增殖培养基，所述增殖培养基是MS+6-苄基嘌呤1.5mg/L+萘乙酸1.5mg/L，培养至长出3～4 cm丛生芽；
E、将步骤D所得长叶长根的幼苗放入阳光下3～5天，取出幼苗，将其移栽至育苗床，行距5～6cm、株距5～6cm，开深3～4cm，每处栽种一株幼苗，保持育苗床湿润；所述育苗床是由甘蔗叶作为基质，混合有蔗糖厂滤泥和/或者鱼塘淤泥、磷酸氢二钾、花生麸、腐殖质、硫酸锰、硫酸锌发酵而得；当幼苗长出6～7片复叶，60%植株出现五出复叶时，将其移栽至大田，即可。
2.根据权利要求1所述的通过根尖培育良种玄参的方法，其特征在于：所述培育阶段的培养条件为温度23～28℃，光照强度1500～1800LUX。
3.根据权利要求1所述的通过根尖培育良种玄参的方法，其特征在于：所述育苗床的各成分比重为甘蔗叶60～75重量份、蔗糖厂滤泥和/或者鱼塘淤泥20～25重量份、磷酸氢二钾5～10重量份、腐殖质5～10重量份、硫酸锰1～5重量份、硫酸锌1～5重量份、花生麸1～2重量份。
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| CN105191799A (zh) * | 2015-09-30 | 2015-12-30 | 耿跃 | 一种玄参组培快繁的方法 |
| CN105309311A (zh) * | 2015-11-06 | 2016-02-10 | 道真仡佬族苗族自治县万福农业综合开发有限公司 | 一种玄参良种繁育方法 |
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