CN106086128A - A kind of preparation method of tuna peptone - Google Patents
A kind of preparation method of tuna peptone Download PDFInfo
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- CN106086128A CN106086128A CN201510696290.2A CN201510696290A CN106086128A CN 106086128 A CN106086128 A CN 106086128A CN 201510696290 A CN201510696290 A CN 201510696290A CN 106086128 A CN106086128 A CN 106086128A
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Abstract
The invention discloses the preparation method of a kind of tuna peptone, with tuna read meat as raw material, be prepared from by double enzyme enzymolysis and flocculent precipitation.The present invention prepares peptone preferable stability, and amino acid content is high, provides sufficient organic nitrogen source for microorganism culturing；Produce simple, beneficially large-scale production；Its production technology has wide market prospect at same product scope.
Description
Technical field
The present invention relates to the preparation method of a kind of tuna peptone, belong to microbiological culture media technical field.
Background technology
Tuna read meat is processing of aquatic products by-product, and its protein content is high, has Biostatic high.
Peptone, is organic compound.Peptone is to form being dried after meat, casein or gelatin acid or protease hydrolysis
Outward appearance be flaxen powder, there is the special breath that meat is fragrant.Protein also can shape after acid, alkali or proteases for decomposing
Become peptone.One of protopepsia product at gastric protein is exactly peptone.Peptone rich in organonitrogen compound,
Also some vitamin and saccharide are contained.It can as the primary raw material of microbiological culture media, antibiotic, medical industry,
Consumption in the fields such as fermentation industry, biochemical product and microbiology scientific research is the biggest, can be used to treat digestive tract disease；
Different organisms needs specific aminoacid and polypeptide, therefore there is various peptone, in general, for albumen
The albumen that peptone produces includes animal proteinum (casein, meat), vegetable protein (beans), microprotein (yeast)
Deng three kinds.The nutrient substance such as C source, N source, somatomedin can be provided for microorganism.
Summary of the invention
The technical problem to be solved in the present invention is to overcome existing defect, it is provided that a kind of good stability, amino acid content are high
The preparation method of tuna peptone；
It is a further object of the present invention to provide said method and prepare tuna peptone.
The purpose of the present invention implements by the following technical programs:
The preparation method of a kind of tuna peptone, with tuna read meat as raw material, by double enzyme enzymolysis and flocculent precipitation
It is prepared from.
The preparation method of above-mentioned tuna peptone, comprises the following steps:
1) reject the impurity such as the fish internal organs in tuna read meat, fishbone, at room temperature cut and mix chopping, obtain red meat meat paste；
2) in tuna read meat meat paste, add water, after stirring and evenly mixing, be warming up to 40～60 DEG C, add ammonia, adjust pH
Value, to 6.0～8.5, adds a protease hydrolyzed 0.5～2h, obtains hydrolyzed solution for the first time；
3) adding phosphoric acid in first time hydrolyzed solution, pH value regulates to 5.0～8.5, adds No. two protease, continues stirring
After enzymolysis 2.5～4.5h, it is warming up to 70～100 DEG C of enzyme denaturing；
4) by the hydrolyzed solution flocculant stratification after inactivation, take the supernatant containing target component and add flocculant, low
Speed stirring；
5) centrifugation insoluble impurities composition, takes clear liquid and carries out solid content detection；
6) clear liquid is concentrated in vacuo, then carries out being spray-dried and dry powder collection.
Preferably, described step 2) in, add the pure water of meat paste quality 1.0-2.5 times quality；Every 1kg red meat meat paste adds
Ammonia 10～50mL；
It is further preferred that described step 2) in, stirring and evenly mixing is also heated to 50～55 DEG C.
Preferably, described step 3) in, add phosphoric acid 5～20mL/kg.
Preferably, described step 2) in, a protease is trypsin, and preferable amount is to add red meat meat paste quality
0.85‰-2.0‰；Described step 3) in, No. two protease of addition are neutral or basic hydrolysis protease, preferably use
Amount is the 0.85 ‰-2.0 ‰ of red meat meat paste quality.
Preferably, described step 4) in, described flocculant is osseocolla, it is further preferred that the consumption of described flocculant is
0.1‰-10‰。
Preferably, described step 5) in, with 4500～6500RPM centrifugations.
Preferably, described step 6) in, it is 50～100 DEG C that temperature is concentrated in vacuo.
Preferably, described step 6) in, it is 65～75 DEG C that temperature is concentrated in vacuo.
Using peptone prepared by the preparation method of above-mentioned tuna peptone, peptone particle diameter is less than or equal to 1.0cm.
The present invention, with tuna read meat as raw material, through secondary deep enzymolysis, separates, concentrates and the technique such as spray drying, system
Obtain safe and stable, the tuna peptone of comprehensive nutrition.Product is slight yellow powdery product, and peptone content is higher, easily
It is dissolved in water.The important measurement index of the peptone such as total nitrogen and amino nitrogen is better than common proteins peptone, and amino acid content is abundant, group
Become comprehensively, cover the indispensable aminoacid of sulfur-containing amino acid such as microorganism culturing and the detection etc. such as (methionine) and tryptophan.
The protein component hydrolysis degree of the peptone that the inventive method obtains, compares animal or plant peptone height thalline and absorbs
Being easier to, residual is less.Additionally, the peptone that the inventive method obtains is rich in multivitamin and various trace element,
The comprehensive advantage of its nutritional labeling be other derived peptone incomparable.And domestic there is no utilizes double enzyme enzymolysis legal system
The research report of standby tuna peptone.
The present invention prepares peptone preferable stability, and amino acid content is high, provides the organic of abundance for microorganism culturing
Nitrogen source；Produce simple, beneficially large-scale production；Its production technology has wide market prospect at same product scope.
Preparation method technique of the present invention is simple, process is easily-controllable, using tuna read meat as raw material carry out secondary deep enzymolysis,
Separating, concentrate and prepared by the technique such as spray drying, gold is without the series of problems of other peptones, and industrial production profit is higher.
The peptone that the method is made is higher for its stability of microorganism culturing, easily absorbs and nonhazardous effect.
Detailed description of the invention
Hereinafter the preferred embodiments of the present invention are illustrated, it will be appreciated that preferred embodiment described herein is only used for
The description and interpretation present invention, is not intended to limit the present invention.
In example below, tuna peptone particle diameter is less than or equal to 0.5cm.
Embodiment 1:
A kind of preparation method of tuna peptone
Step (1). take 1000g tuna read meat, cut at 20 DEG C and mix chopping, obtain red meat meat paste；
Step (2). in tuna read meat meat paste, add the appropriate 1.5L of water, after stirring and evenly mixing, be warming up to 40 DEG C, ammonification
Water 21.3mL, adjustment ph value, to about 8.0, adds Novi letter trypsin 1.0g, enzymolysis 1.0h, obtains water for the first time
Solve liquid；
Step (3). adding phosphoric acid 8.3mL in first time hydrolyzed solution, ph value regulates to 5.5, adds Novi's letter hydrolyzed protein
Enzyme 1.0g, after continuing stirring enzymolysis 2.0h, is warming up to 80 DEG C of enzyme denaturing；
Step (4). by the hydrolyzed solution flocculant stratification after inactivation, take the supernatant containing target component and add 2 ‰ bones
Glue, stirring at low speed；
Step (5). with 4500RPM centrifugation insoluble impurities composition, take clear liquid and carry out solid content detection；
Step (6). solid content is carried out 65 DEG C be concentrated in vacuo after, finally carry out be spray-dried and dry powder collect.
The peptone prepared of this embodiment is flaxen powder, after measured: wherein nitrogen content is 9%, alpha-amido
Nitrogen content is 1.8%, and content of ashes is 10%.
Embodiment 2:
A kind of preparation method of tuna peptone
Step (1). take tuna read meat 250kg, cut at 22 DEG C and mix chopping, obtain red meat meat paste；
Step (2). in tuna read meat meat paste, add water in right amount, after stirring and evenly mixing, be warming up to 55 DEG C, add ammonia 4.5L,
Adjustment ph value, to about 8.5, adds Novi letter trypsin 250g, enzymolysis 1h, obtains hydrolyzed solution for the first time；
Step (3). adding phosphoric acid 4.7L in first time hydrolyzed solution, ph value regulates to 5.6, adds Novi's menstruation proteolytic enzyme
250g, after continuing stirring enzymolysis 4.5h, is warming up to 100 DEG C of enzyme denaturing；
Step (4). by the hydrolyzed solution flocculant stratification after inactivation, take the supernatant containing target component and add 500g
Osseocolla, stirring at low speed；
Step (5). stand cooling, use plate-and-frame filtration to carry out roguing separation, take clear liquid and carry out solid content detection；
Step (6). after solid content carries out 75 DEG C of vacuum films concentrations, finally carry out being spray-dried and dry powder collection.
The peptone that this embodiment prepares is flaxen powder, and its 2% concentrated solution is transparence liquid, after measured: its
Nitrogen content is 10%, and alpha-amino nitrogen content is 2.0%, and content of ashes is 9.8%.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to aforementioned enforcement
The present invention has been described in detail by example, and for a person skilled in the art, it still can be to aforementioned each enforcement
Technical scheme described in example is modified, or wherein portion of techniques feature is carried out equivalent.All the present invention's
Within spirit and principle, any modification, equivalent substitution and improvement etc. made, should be included in protection scope of the present invention
Within.
Claims (10)
1. the preparation method of a tuna peptone, it is characterised in that: with tuna read meat as raw material, by double enzyme enzymolysis
And flocculent precipitation is prepared from.
The preparation method of tuna peptone the most according to claim 1, it is characterised in that: comprise the following steps:
1) reject the impurity such as the fish internal organs in tuna read meat, fishbone, at room temperature cut and mix chopping, obtain red meat meat paste；
2) in tuna read meat meat paste, add water, after stirring and evenly mixing, be warming up to 40～60 DEG C, add ammonia, adjust pH value extremely
6.0～8.5, add a protease hydrolyzed 0.5～2h, obtain hydrolyzed solution for the first time；
3) adding phosphoric acid in first time hydrolyzed solution, pH value regulates to 5.0～8.5, adds No. two protease, continues stirring enzymolysis
After 2.5～4.5h, it is warming up to 70～100 DEG C of enzyme denaturing；
4) by the hydrolyzed solution flocculant stratification after inactivation, taking the supernatant containing target component and add flocculant, low speed stirs
Mix；
5) centrifugation insoluble impurities composition, takes clear liquid and carries out solid content detection；
6) clear liquid is concentrated in vacuo, then carries out being spray-dried and dry powder collection.
The preparation method of tuna peptone the most according to claim 2, it is characterised in that: described step 2) in, add
Enter the pure water of meat paste quality 1.0-2.5 times quality；Every 1kg red meat meat paste adds ammonia 10～50mL；
Preferably, described step 2) in, stirring and evenly mixing is also heated to 50～55 DEG C.
4. according to the preparation method of the tuna peptone described in Claims 2 or 3, it is characterised in that: described step 3) in,
Add phosphoric acid 5～20mL/kg.
The preparation method of tuna peptone the most according to claim 2, it is characterised in that: described step 2) in, one
Number protease is trypsin, and preferable amount is add red meat meat paste quality 0.85 ‰-2.0 ‰；Described step 3) in, add
No. two protease entered are neutral or basic hydrolysis protease, and preferable amount is the 0.85 ‰-2.0 ‰ of red meat meat paste quality.
The preparation method of tuna peptone the most according to claim 2, it is characterised in that: described step 4) in, institute
Stating flocculant is osseocolla, it is preferred that the consumption of described flocculant is 0.1 ‰-10 ‰.
The preparation method of tuna peptone the most according to claim 2, it is characterised in that: described step 5) in, use
4500～6500RPM centrifugations.
The preparation method of tuna peptone the most according to claim 2, it is characterised in that: described step 6) in, very
Empty thickening temperature is 50～100 DEG C.
The preparation method of tuna peptone the most according to claim 8, it is characterised in that: described step 6) in, very
Empty thickening temperature is 65～75 DEG C.
10. the peptone prepared according to the preparation method of the tuna peptone described in any one of claim 1-9, its feature exists
In: peptone particle diameter is less than or equal to 1.0cm.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834401A (en) * | 2017-01-23 | 2017-06-13 | 舟山福氏食品科技有限公司 | A kind of small band fish peptone production technology |
| CN107226855A (en) * | 2017-07-11 | 2017-10-03 | 浙江丰宇海洋生物制品有限公司 | A kind of peptone of the molten slurry production of utilization fish |
| CN107227332A (en) * | 2017-07-11 | 2017-10-03 | 浙江丰宇海洋生物制品有限公司 | A kind of method of the molten slurry production peptone of utilization fish |
| CN107513548A (en) * | 2017-10-31 | 2017-12-26 | 荣成市日鑫水产有限公司 | A kind of method that peptone is prepared using fish meal pressed liquor |
| CN107988300A (en) * | 2017-12-06 | 2018-05-04 | 浙江海洋大学 | A kind of preparation method of the antifatigue polypeptide of tuna read meat |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102827911A (en) * | 2012-09-24 | 2012-12-19 | 平凉市华科生物技术有限公司 | Preparation method of animal peptone by double-enzyme enzymolysis |
| CN103276038A (en) * | 2013-05-13 | 2013-09-04 | 贵州新华生化科技发展有限公司 | Method for producing reagent grade peptone |
| CN104342474A (en) * | 2014-10-15 | 2015-02-11 | 浙江海洋学院 | Comprehensive utilization method of tuna leftovers |
-
2015
- 2015-10-23 CN CN201510696290.2A patent/CN106086128A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102827911A (en) * | 2012-09-24 | 2012-12-19 | 平凉市华科生物技术有限公司 | Preparation method of animal peptone by double-enzyme enzymolysis |
| CN103276038A (en) * | 2013-05-13 | 2013-09-04 | 贵州新华生化科技发展有限公司 | Method for producing reagent grade peptone |
| CN104342474A (en) * | 2014-10-15 | 2015-02-11 | 浙江海洋学院 | Comprehensive utilization method of tuna leftovers |
Non-Patent Citations (1)
| Title |
|---|
| F. GUE´RARD ET AL.: "Enzymatic hydrolysis of proteins from yellowfin tuna (Thunnus albacores) wastes using Alcalase", 《JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834401A (en) * | 2017-01-23 | 2017-06-13 | 舟山福氏食品科技有限公司 | A kind of small band fish peptone production technology |
| CN107226855A (en) * | 2017-07-11 | 2017-10-03 | 浙江丰宇海洋生物制品有限公司 | A kind of peptone of the molten slurry production of utilization fish |
| CN107227332A (en) * | 2017-07-11 | 2017-10-03 | 浙江丰宇海洋生物制品有限公司 | A kind of method of the molten slurry production peptone of utilization fish |
| CN107513548A (en) * | 2017-10-31 | 2017-12-26 | 荣成市日鑫水产有限公司 | A kind of method that peptone is prepared using fish meal pressed liquor |
| CN107513548B (en) * | 2017-10-31 | 2021-01-22 | 荣成市日鑫水产有限公司 | Method for preparing peptone by using fish meal squeezed liquid |
| CN107988300A (en) * | 2017-12-06 | 2018-05-04 | 浙江海洋大学 | A kind of preparation method of the antifatigue polypeptide of tuna read meat |
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