CN106834401A - A kind of small band fish peptone production technology - Google Patents
A kind of small band fish peptone production technology Download PDFInfo
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- CN106834401A CN106834401A CN201710049294.0A CN201710049294A CN106834401A CN 106834401 A CN106834401 A CN 106834401A CN 201710049294 A CN201710049294 A CN 201710049294A CN 106834401 A CN106834401 A CN 106834401A
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- fish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- Proteomics, Peptides & Aminoacids (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention discloses a kind of preparation method of small band fish peptone, comprise the following steps：1) impurity such as fish guts, fish-bone, fin in the rejecting small band flesh of fish, cuts mix chopping at room temperature, obtains meat gruel；2) through petroleum ether or ether defatting, meat liquid volume ratio is 2 to the meat gruel that will be obtained:1‑3:1, obtain minced fillet slurry；3) the minced fillet slurry that will be obtained obtains suspension through 12 parts of 0.1 0.2% papain, 50 60 DEG C of 4 6h of enzymolysis；4) suspension that will be obtained is centrifuged 8 16min through 10000 15000r/min, collects supernatant；5) supernatant that will be obtained carries out desalination, concentration and is spray-dried using 250 400Da NF membranes.The present invention prepares peptone preferable stability, and amino acid content is high, and the organic nitrogen source of abundance is provided for microculture；Production is simple, is conducive to large-scale production；Its production technology has wide market prospects in same product scope.
Description
Technical field
The present invention relates to a kind of peptone processing technology, more particularly, to a kind of small band fish peptone production technology.
Background technology
Peptone, is organic compound.Peptone is to form drying after the acid of meat, casein or gelatin or protease hydrolytic
Outward appearance be in flaxen pulvis, with the special breath that meat is fragrant.Protein can also form egg after acid, alkali or proteases for decomposing
White peptone.One of protopepsia product in stomach internal protein is exactly peptone.Peptone is rich in organonitrogen compound, also contains one
A little vitamins and carbohydrate.It can be as the primary raw material of microbiological culture media, in antibiotic, medical industry, fermentation industry, life
The consumption changed in the field such as product and microbiology scientific research is very big, can be used to treat disease of digestive tract；Different organisms
Specific amino acid and polypeptide are needed, therefore there are various peptones, in general, the albumen for peptone production includes
Three kinds of animal protein (casein, meat), vegetable protein (beans), microprotein (yeast) etc..C can be provided for microorganism
The nutriments such as source, N sources, growth factor.
The production technology of traditional protein peptone is main, and to raise bone as raw material, the raw material for being actually used in production peptone is a lot,
After the livestock blood, the fish scrap of aquatic product manufactory offer, the biochemical pharmaceutical factory extraction organotherapeutic medicament that are provided such as slaughterhouse
Glue residue, the steaming bone water of skeletal grain factory and fish meal after residue, Jiao Chang production gelatin etc., can be used as the original of production peptone
Material.At present there are some researches show in peptone products prepared by different material, the enzymolysis product of fish protein resource has protein
The features such as content is high, good water solubility, polypeptide and free amino acid are abundant, therefore, fish peptone can be used as fermentation industry and experiment
The optimum protein peptone that microculture is selected in room.
The quality of peptone is notable to the growth effect of microorganism, so that the stability contorting in influenceing microbial cultivation process
And the qualitative and quantitative determination of microorganism.Research and development it is a kind of it is quick suitable, be conducive to mass producing and having extensively in same product scope
The peptone production technology of wealthy market prospects turns into our a big target.
The content of the invention
The technical problems to be solved by the invention are to provide that a kind of stability is high, amino acid content is high, production is simple small
Band fish peptone and production technology.The present invention solve the technical scheme that is used of above-mentioned technical problem for：
A kind of small band fish peptone production technology, comprises the following steps：
1) impurity such as fish guts, fish-bone, fin in the rejecting small band flesh of fish, cuts mix chopping at room temperature, obtains meat gruel；
2) through petroleum ether or ether defatting, meat liquid volume ratio is 2 to the meat gruel that will be obtained:1-3:1, obtain minced fillet slurry；
3) through 1-2 part of 0.1-0.2% papains, 50-60 DEG C digests 4-6h to the minced fillet slurry that will be obtained, and obtains suspension；
4) suspension that will be obtained is centrifuged 8-16min through 10000-15000r/min, collects supernatant；
5) supernatant that will be obtained carries out desalination, concentration and is spray-dried using 250-400Da NF membranes.
Double property contracting amino acids of the degreasing agent used also barium sulfate containing 0.01-0.1% and 0.01-0.1%.
Preferably, above-mentioned skimming processes select petroleum ether, and meat liquor ratio is 2:1.
Further preferably, above-mentioned enzymolysis process selects 1 part of 0.1% papain, 55 DEG C of enzymolysis 5h.
Further preferably, above-mentioned centrifugal process is centrifuged 10min from 12000r/min.
Further preferably, above-mentioned NF membrane uses 300Da meshes.
The present invention prepares peptone stability higher, and amino acid content is high, for microculture provides having for abundance
Machine nitrogen source；Wherein barium sulfate and double property contracting amino acids make peptone have stability higher, and production is simple, are conducive to extensive
Production；Its production technology has wide market prospects in same product scope.Invention preparation method process is simple, process are easily-controllable,
The techniques such as secondary deep enzymolysis, separation, concentration and spray drying are carried out using tuna read meat as raw material to prepare, gold is without other eggs
The series of problems of white peptone, industrial production profit is higher.Its stability is higher for microculture for the peptone that the method is made,
Easily absorb and nonhazardous effect.
Specific embodiment
The present invention is described in further detail with reference to embodiments.
Embodiment 1
A kind of production technology of small band fish peptone, comprises the following steps：
1) impurity such as fish guts, fish-bone, fin in the rejecting small band flesh of fish, cuts mix chopping at room temperature, obtains meat gruel；
2) through petroleum ether or ether defatting, meat liquid volume ratio is 2 to the meat gruel that will be obtained:1-3:1, obtain minced fillet slurry；Used is de-
Double property contracting amino acids of fat the agent also barium sulfate containing 0.01-0.1% and 0.01-0.1%；
3) through 1-2 part of 0.1-0.2% papains, 50-60 DEG C digests 4-6h to the minced fillet slurry that will be obtained, and obtains suspension；
4) suspension that will be obtained is centrifuged 8-16min through 10000-15000r/min, collects supernatant；
5) supernatant that will be obtained carries out desalination, concentration and is spray-dried using 250-400Da NF membranes.
The obtained peptone of this embodiment is flaxen powder, after measured：Wherein nitrogen content is 9%, alpha-amido
Nitrogen content is 1.8%, and content of ashes is 10%.
Embodiment 2
The production technology of small band fish peptone, comprises the following steps：
1) impurity such as fish guts, fish-bone, fin in the rejecting small band flesh of fish, cuts mix chopping at room temperature, obtains meat gruel；
2) through petroleum ether degreasing, meat liquid volume ratio is 2 to the meat gruel that will be obtained:1, obtain minced fillet slurry；Degreasing agent used also contains
0.01% barium sulfate and 0.01% double property contracting amino acids；
3) the minced fillet slurry that will be obtained obtains suspension through 1 part of 0.1% papain, 50 DEG C of enzymolysis 6h；
4) suspension that will be obtained is centrifuged 16min through 10000r/min, collects supernatant；
5) supernatant that will be obtained carries out desalination, concentration and is spray-dried using 250Da NF membranes.
Peptone obtained in this embodiment is flaxen powder, and its 2% concentrate is transparence liquid, after measured：
Its nitrogen content is 10%, and AAN content is 2.0%, and content of ashes is 9.8%.
Embodiment 3
The production technology of small band fish peptone, comprises the following steps：
1) impurity such as fish guts, fish-bone, fin in the rejecting small band flesh of fish, cuts mix chopping at room temperature, obtains meat gruel；
2) through petroleum ether degreasing, meat liquid volume ratio is 3 to the meat gruel that will be obtained:1, obtain minced fillet slurry；
3) the minced fillet slurry that will be obtained obtains suspension through 2 parts of 0.2% papain, 50 DEG C of enzymolysis 4h；
4) suspension that will be obtained is centrifuged 8min through 15000r/min, collects supernatant；
5) supernatant that will be obtained carries out desalination, concentration and is spray-dried using 400Da NF membranes.
Peptone obtained in this embodiment is flaxen powder, and its 2% concentrate is transparence liquid, after measured：
Its nitrogen content is 10%, and AAN content is 2.0%, and content of ashes is 9.8%.
The preferred embodiments of the present invention are the foregoing is only, is not intended to limit the invention, although with reference to foregoing reality
Apply example to be described in detail the present invention, for a person skilled in the art, it still can be to foregoing each implementation
Technical scheme described in example is modified, or carries out equivalent to which part technical characteristic.It is all in essence of the invention
Within god and principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (6)
1. a kind of production technology of small band fish peptone, it is characterised in that：Comprise the following steps：
1) impurity such as fish guts, fish-bone, fin in the rejecting small band flesh of fish, cuts mix chopping at room temperature, obtains meat gruel；
2) through petroleum ether or ether defatting, meat liquid volume ratio is 2 to the meat gruel that will be obtained:1-3:1, obtain minced fillet slurry；
3) through 1-2 part of 0.1-0.2% papains, 50-60 DEG C digests 4-6h to the minced fillet slurry that will be obtained, and obtains suspension；
4) suspension that will be obtained is centrifuged 8-16min through 10000-15000r/min, collects supernatant；
5) supernatant that will be obtained carries out desalination, concentration and is spray-dried using 250-400Da NF membranes.
2. the production technology of small band fish peptone according to claim 1, it is characterised in that：Described step 2) in, institute
Double property contracting amino acids of the degreasing agent also barium sulfate containing 0.01-0.1% and 0.01-0.1%.
3. the production technology of small band fish peptone according to claim 1, it is characterised in that：Described step 2) in, meat
Rotten is 2 with petroleum ether or ether volume ratio:1-3:1.
4. the production technology of small band fish peptone according to claim 1, it is characterised in that：Described step 3) in need
Plus 1-2 parts of 0.1-0.2% papains, 50-60 DEG C of enzymolysis 4-6h.
5. the production technology of small band fish peptone according to claim 2, it is characterised in that：Described step 4) need through
10000-15000r/min is centrifuged 8-16min.
6. the production technology of small band fish peptone according to claim 1, it is characterised in that：Described step 5) need to adopt
Desalination, concentration are carried out with 250-400Da NF membranes and is spray-dried.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710049294.0A CN106834401A (en) | 2017-01-23 | 2017-01-23 | A kind of small band fish peptone production technology |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710049294.0A CN106834401A (en) | 2017-01-23 | 2017-01-23 | A kind of small band fish peptone production technology |
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| CN106834401A true CN106834401A (en) | 2017-06-13 |
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| CN201710049294.0A Pending CN106834401A (en) | 2017-01-23 | 2017-01-23 | A kind of small band fish peptone production technology |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107226855A (en) * | 2017-07-11 | 2017-10-03 | 浙江丰宇海洋生物制品有限公司 | A kind of peptone of the molten slurry production of utilization fish |
| CN107227332A (en) * | 2017-07-11 | 2017-10-03 | 浙江丰宇海洋生物制品有限公司 | A kind of method of the molten slurry production peptone of utilization fish |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823886A (en) * | 2011-06-16 | 2012-12-19 | 浙江兴业集团有限公司 | Method for producing high-quality minced hairtail |
| CN104313093A (en) * | 2014-10-17 | 2015-01-28 | 福州大学 | Method for preparing peptone by utilizing fish processing wastes |
| CN106086128A (en) * | 2015-10-23 | 2016-11-09 | 浙江海洋学院 | A kind of preparation method of tuna peptone |
-
2017
- 2017-01-23 CN CN201710049294.0A patent/CN106834401A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823886A (en) * | 2011-06-16 | 2012-12-19 | 浙江兴业集团有限公司 | Method for producing high-quality minced hairtail |
| CN104313093A (en) * | 2014-10-17 | 2015-01-28 | 福州大学 | Method for preparing peptone by utilizing fish processing wastes |
| CN106086128A (en) * | 2015-10-23 | 2016-11-09 | 浙江海洋学院 | A kind of preparation method of tuna peptone |
Non-Patent Citations (2)
| Title |
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| 郑允权 等: "鱼蛋白胨的制备与质量分析", 《中国生物制品学杂志》 * |
| 陈友德: "纯带鱼蛋白胨", 《今日科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107226855A (en) * | 2017-07-11 | 2017-10-03 | 浙江丰宇海洋生物制品有限公司 | A kind of peptone of the molten slurry production of utilization fish |
| CN107227332A (en) * | 2017-07-11 | 2017-10-03 | 浙江丰宇海洋生物制品有限公司 | A kind of method of the molten slurry production peptone of utilization fish |
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