CN103911413A - Method for preparing selenium-rich rice polypeptide - Google Patents
Method for preparing selenium-rich rice polypeptide Download PDFInfo
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- CN103911413A CN103911413A CN201410139376.0A CN201410139376A CN103911413A CN 103911413 A CN103911413 A CN 103911413A CN 201410139376 A CN201410139376 A CN 201410139376A CN 103911413 A CN103911413 A CN 103911413A
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Abstract
The invention provides a method for preparing selenium-rich rice polypeptide. The method comprises the following steps: (1) picking up fresh and high-quality selenium-rich rice, removing impurities, elutriating the selenium-rich rice until no turbid water exits, and grinding the selenium-rich rice into powder; (2) adding selenium-rich grain stillage left after wine production; (3) adding selenium-rich yeast which is obtained by virtue of a microbial strain technology, firstly picking up a yeast colony capable of converting inorganic selenium into organic selenium by using the inorganic selenium near a selenium ore, carrying out separating and enrichment culturing on the yeast colony to obtain a pure yeast specie; (4) putting the selenium-rich rice in a cylinder and fermenting; (5) filtering and detecting; (6) extracting selenium-rich rice polypeptide, namely extracting polypeptide from filtrate by adopting an electrophoretic separation method. The selenium-rich rice polypeptide extracted by the method provided by the invention is prepared from a pure natural raw material and has an obvious biological activity effect, meanwhile, the raw material is waste grain stillage left after wine production, so that the production cost is low, and sources and energy sources can be effectively saved.
Description
Technical field
The present invention is a kind of preparation method of the large mpd polypeptide that is rich in organic active selenium.
Background technology
Large mpd polypeptide is the hydrolysate of rice protein, and its amino acid composition is about the same with rice protein, and its indispensable amino acid proportional balancing method and rich content.It has the physiological function more superior than rice protein and processing characteristics, and this has expanded the Application Areas of rice protein greatly.And selenium-rich rice polypeptide is to be rich in organic active selenium make large mpd polypeptide under the prerequisite of large mpd polypeptide in.Organoselenium generally exists with the form of Sethotope.Large mpd polypeptide has the Physiological Properties of many uniquenesses, and as in human body, large mpd polypeptide is than rice protein absorption more easy to digest, antigenicity is lower; There is the metabolism of the body fat mass in human body of promotion, the erythrocytic recovery of flesh, strengthen sportsmen's muscle function; Step-down, decreasing cholesterol function, and promote fermentation, make food gel softening function.And selenium-rich rice polypeptide has not only been inherited all functions of general rice polypeptide, be also rich in the favourable organoselenium of pair human body.
Scientific research shows, selenium is the trace element of needed by human, and in the resident in state-owned about seventy percent area lack selenium.The mankind lack selenium can cause the various diseases such as cancer stricken, diabetes, cardiovascular and cerebrovascular disease, and this has become common recognition in medical circle, and countries in the world are determined every 4 years and held the once international symposium about selenium for this reason.Lack the situation of selenium for human body, the Devoting Major Efforts To Developing of various countries' expert advice continuation is at present developed rich selenium product.The selenium consumption that China is recommended for different crowd is: micro-gram/day of prevention deficiency disease 50, and subhealth state health care is measured 100 micro-grams/day, and the great chronic disease such as preventing cancer is intervened micro-gram/day of dosage 200, intervenes dosage and be greater than 200 micro-grams/day during treatment.
At present, a lot of enterprises and R&D institution all make great efforts to set about to make to contain in large mpd polypeptide higher organoselenium at links, but go back at present inorganic selenium that neither one technical process can make to add in large mpd polypeptide and be all converted into organoselenium, and remaining inorganic selenium there is certain toxicity to human body.
Summary of the invention
The object of the present invention is to provide one can reduce antigenicity, reduce blood sugar, cholesterol, strengthen muscle function, strengthens immune function of human body, the preparation method of the selenium-rich rice polypeptide of protection cardiovascular and cerebrovascular and anti-oxidant anti-aging effect.
To achieve these goals, technical problem solved by the invention realizes by the following technical solutions:
A preparation method for selenium-rich rice polypeptide, comprises the following steps:
(1) select the selenium-rich rice of fresh high-quality, remove impurity, eluriate to without obvious muddy water, then selenium-rich rice is milled into powder;
(2) add self-control vinasse
Self-control vinasse are after processing by selenium-rich rice, to add homemade yeast rich in selenium fermentation to produce the rich selenium vinasse that are left behind after drinking;
(3) add yeast rich in selenium
Yeast rich in selenium utilizes microorganism strains filtration technology to obtain, first near selenium ore deposit, pick out the yeast colony that can utilize inorganic selenium and inorganic selenium is converted to organoselenium, then separation, enrichment culture are pure barms, be domesticated for again yeast rich in selenium, then select by selecting that to make protein degradation be polypeptide but can be thoroughly degraded to amino acid whose yeast rich in selenium in certain degradation capability scope;
(4) lower cylinder, fermentation;
(5) filter, detect;
The trichloroacetic acid solution of employing 15% precipitates high molecular weight protein in sample completely, and small-molecular peptides is retained in this solution simultaneously, removes albumen precipitation, and supernatant liquor is measured total nitrogen content according to the method for GB/T5009.5-2003 regulation;
(6) extract selenium-rich rice polypeptide
The method that adopts electrophoretic separation extracts polypeptide from filtered liquid.
The weight part composition of each component in described batching: selenium-rich rice 8-10 part, vinasse 82-85 part, yeast rich in selenium 5-10 part.
The temperature of described fermentation is 25-28 ℃.
beneficial effect
it is raw material that the present invention utilizes the rich selenium vinasse of high-quality, selects the high-efficient selenium-rich yeast of autonomous screening, extracts to obtain selenium-rich rice polypeptide through improvement zymotechnique flow process; This polypeptide is made up of pure natural raw material, has obvious biological active and answers, and can strengthen the immunological competence of human body, cancer-resisting; Have liver-protective effect simultaneously, can alleviate the operating pressure of liver, antigenicity is lower; There is the metabolism of the body fat mass in human body of promotion, the erythrocytic recovery of flesh, strengthen sportsmen's muscle function; Step-down, decreasing cholesterol function, the effect that also there is protection cardiovascular and cerebrovascular and recover eyesight; Raw material is the useless vinasse that produce after drinking in addition, and production cost is low, can effectively economize on resources, and the energy.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Based on the embodiment in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
A preparation method for selenium-rich rice polypeptide, comprises the following steps:
(1) select the selenium-rich rice of fresh high-quality, remove impurity, eluriate to without obvious muddy water, then selenium-rich rice is milled into powder;
(2) add self-control vinasse
Self-control vinasse are after processing by selenium-rich rice, to add homemade yeast rich in selenium fermentation to produce the rich selenium vinasse that are left behind after drinking;
(3) add yeast rich in selenium
Yeast rich in selenium utilizes microorganism strains filtration technology to obtain, first near selenium ore deposit, pick out the yeast colony that can utilize inorganic selenium and inorganic selenium is converted to organoselenium, then separation, enrichment culture are pure barms, be domesticated for again yeast rich in selenium, then select by selecting that to make protein degradation be polypeptide but can be thoroughly degraded to amino acid whose yeast rich in selenium in certain degradation capability scope;
(4) lower cylinder, fermentation;
(5) filter, detect;
The trichloroacetic acid solution of employing 15% precipitates high molecular weight protein in sample completely, and small-molecular peptides is retained in this solution simultaneously, removes albumen precipitation, and supernatant liquor is measured total nitrogen content according to the method for GB/T5009.5-2003 regulation;
(6) extract selenium-rich rice polypeptide
The method that adopts electrophoretic separation extracts polypeptide from filtered liquid.
The weight part composition of each component in described batching: selenium-rich rice 8-10 part, vinasse 82-85 part, yeast rich in selenium 5-10 part.
The temperature of described fermentation is 25-28 ℃.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and in the situation that not deviating from spirit of the present invention or essential characteristic, can realize the present invention with other specific form.Therefore, no matter from which point, all should regard embodiment as exemplary, and be nonrestrictive, scope of the present invention is limited by claims rather than above-mentioned explanation, is therefore intended to all changes that drop in the implication and the scope that are equal to important document of claim to include in the present invention.
In addition, be to be understood that, although this specification sheets is described according to embodiment, but be not that each embodiment only comprises an independently technical scheme, this narrating mode of specification sheets is only for clarity sake, those skilled in the art should make specification sheets as a whole, and the technical scheme in each embodiment also can, through appropriately combined, form other embodiments that it will be appreciated by those skilled in the art that.
Claims (3)
1. a preparation method for selenium-rich rice polypeptide, is characterized in that, comprises the following steps:
(1) select the selenium-rich rice of fresh high-quality, remove impurity, eluriate to without obvious muddy water, then selenium-rich rice is milled into powder;
(2) add self-control vinasse
Self-control vinasse are after processing by selenium-rich rice, to add homemade yeast rich in selenium fermentation to produce the rich selenium vinasse that are left behind after drinking;
(3) add yeast rich in selenium
Yeast rich in selenium utilizes microorganism strains filtration technology to obtain, first near selenium ore deposit, pick out the yeast colony that can utilize inorganic selenium and inorganic selenium is converted to organoselenium, then separation, enrichment culture are pure barms, be domesticated for again yeast rich in selenium, then select by selecting that to make protein degradation be polypeptide but can be thoroughly degraded to amino acid whose yeast rich in selenium in certain degradation capability scope;
(4) lower cylinder, fermentation;
(5) filter, detect;
The trichloroacetic acid solution of employing 15% precipitates high molecular weight protein in sample completely, and small-molecular peptides is retained in this solution simultaneously, removes albumen precipitation, and supernatant liquor is measured total nitrogen content according to the method for GB/T5009.5-2003 regulation;
(6) extract selenium-rich rice polypeptide
The method that adopts electrophoretic separation extracts polypeptide from filtered liquid.
2. the preparation method of a kind of selenium-rich rice polypeptide according to claim 1, is characterized in that, the weight part composition of each component in described batching: selenium-rich rice 8-10 part, vinasse 82-85 part, yeast rich in selenium 5-10 part.
3. the preparation method of a kind of selenium-rich rice polypeptide according to claim 1, is characterized in that, the temperature of described fermentation is 25-28 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410139376.0A CN103911413A (en) | 2014-04-09 | 2014-04-09 | Method for preparing selenium-rich rice polypeptide |
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| CN201410139376.0A CN103911413A (en) | 2014-04-09 | 2014-04-09 | Method for preparing selenium-rich rice polypeptide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104543396A (en) * | 2015-01-13 | 2015-04-29 | 香红星 | Method for producing rice polypeptide by adopting solid state fermentation |
| CN108504620A (en) * | 2017-02-28 | 2018-09-07 | 中乔大三农智慧农业（北京）有限公司 | A kind of culture medium and its cultural method of selenium-rich cordyceps |
| CN108689744A (en) * | 2017-02-28 | 2018-10-23 | 中乔大三农智慧农业（北京）有限公司 | The culture medium and its cultural method of selenium-rich cordyceps |
-
2014
- 2014-04-09 CN CN201410139376.0A patent/CN103911413A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104543396A (en) * | 2015-01-13 | 2015-04-29 | 香红星 | Method for producing rice polypeptide by adopting solid state fermentation |
| CN108504620A (en) * | 2017-02-28 | 2018-09-07 | 中乔大三农智慧农业（北京）有限公司 | A kind of culture medium and its cultural method of selenium-rich cordyceps |
| CN108689744A (en) * | 2017-02-28 | 2018-10-23 | 中乔大三农智慧农业（北京）有限公司 | The culture medium and its cultural method of selenium-rich cordyceps |
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Application publication date: 20140709 |