CN102793726B - Process for manually culturing silkworm larva maggots - Google Patents
Process for manually culturing silkworm larva maggots Download PDFInfo
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Abstract
The invention discloses a process for manually culturing silkworm larva maggots, which relates to a process for manually culturing insects. Specific to the limitation on the use of silkworm larvae and the influence of processing, a process for manually culturing silkworm larva maggots comprises the following steps of: (1) smashing silkworm larvae into powder; (2) mixing with water and stirring into paste; (3) sterilizing the paste at a high temperature; (4) treating grain bran with Chinese medicines to generate maggot eggs; (5) culturing young maggots; (6) culturing maggots; (7) separating maggots, cleaning and inactivating; (8) drying maggots; and (9) packing maggots. A biotransformation way is adopted, and allergens and toxins of stiff silkworms are eliminated in the culturing process of stiff silkworm maggots, so that the application range of the stiff silkworms is expanded; and the stiff silkworm maggots serving as a novel Chinese medicinal raw material has the advantages of definite composition, nontoxicity and tastelessness, so that more space is provided for the application of stiff silkworms. A technical process is easy to control, energy is saved, and the process is suitable for industrial production.
Description
Technical field
The present invention relates to the technique of artificial culture insect, particularly the technique of artificial culture larva of a silkworm with batrytis maggot.
Background technology
The larva of a silkworm with batrytis is a kind of traditional traditional Chinese medicine, and the larva of a silkworm with batrytis is that Bombycidae animal bombyx mori larva infects white muscardine fungi and ossified full worm.The larva of a silkworm with batrytis contains 28 kinds of trace elements such as protein, ammonium oxalate, 17 seed amino acids, calcium, magnesium and abnormal active hormones, ecdysone.Larva of a silkworm with batrytis thalline contains soft beauvericin, contains the compositions such as multiple ring acetal acid peptide, fatty acid amide lipoid.
CN101810689A discloses a kind of formula of batryticated silkworm processed by gall bladder, comprises that stiff silkworm, the coptis, ox gall bladder, cicada slough form; CN102153619A discloses a kind of small-molecular weight glycopeptide that extracts, its extraction separation method and purposes from stiff silkworm, separate to get the active ingredient glycopeptide by extraction, ultrafiltration, the concentrated extraction with the ethanol precipitation after the stiff silkworm of traditional Chinese medicine is pulverized; CN101062067 discloses muscardine silkworm skin recovery powder, is comprised of according to a certain ratio stiff silkworm, the root of Dahurain angelica, centipede, scorpio.
ZL20041003742.4 discloses and has adopted Chinese medicine to cultivate the method for toad maggot, adopts the method suitability for industrialized production toad maggot in batches.
The larva of a silkworm with batrytis is owing to being that the bombyx mori larva infects white muscardine fungi and ossified full worm, white muscardine fungi has an immense impact on to it, allergy is a kind of important behaviour, so the larva of a silkworm with batrytis often needs the methods such as frying, bran stir-fry, ginger system, wine system, liquorice beverage system to process when using, and all need high-temperature heating treatment, effective medicinal components is exerted an influence, reduced drug effect.
Summary of the invention
For the limitation of using the larva of a silkworm with batrytis and the impact of the process of preparing Chinese medicine, the invention provides the technique of a kind of artificial culture larva of a silkworm with batrytis maggot.
The objective of the invention is to solve by the following technical programs: (1) larva of a silkworm with batrytis is crushed to Powdered; (2) be mixed into larva of a silkworm with batrytis pastel with water; (3) larva of a silkworm with batrytis pastel high temperature sterilization; (4) cereal chaff and larva of a silkworm with batrytis pastel are processed with Chinese medicine, and generate the maggot ovum; (5) the young maggot culture fluid of preparation carries out the training of young maggot and grows; (6) preparation maggot culture fluid carries out the maggot cultivation; (7) maggot separation, cleaning, deactivation; (8) maggot is dry; (9) maggot packing.Concrete steps are as follows:
(1) larva of a silkworm with batrytis be crushed to Powdered,
(2) be mixed into larva of a silkworm with batrytis pastel with water;
(3) larva of a silkworm with batrytis pastel high temperature sterilization;
(4) cereal bran and larva of a silkworm with batrytis pastel are processed with Chinese medicine and are formed egg cultivation liquid, described egg cultivation liquid is mixed by 100g cereal bran, larva of a silkworm with batrytis pastel 0.2-50g, Ground Cloves 0-10g, rhizoma atractylodis powder 0-5g, wrinkled giant hyssop 0-8g, 0-20g banksia rose powder and 0-5.0g radix aconiti agrestis powder, keep 30-55 ℃ of temperature, time 48-240h, generate worm's ovum;
(5) young maggot training is grown: prepare young maggot culture fluid, described young maggot culture fluid is mixed by 1-100g larva of a silkworm with batrytis pastel, 0-200g peptone, 5-10g yeast extracting powder, 0-10g banksia rose powder, 0-10g wrinkled giant hyssop, 0-30g rhizoma atractylodis powder, 0-20g Chinese medicine Ground Cloves, 2-10gNaCl or KCl, 0-100g soybean protein isolate, 0.5-1.0L pure water, be sub-packed in conical flask, be respectively charged into the worm's ovum that the front training is grown after sterilization, shaking table 1-100r/min, cultivate 12-48h, generate the culture fluid that contains larva of a silkworm with batrytis children maggot;
(6) maggot is cultivated: the preparation maggot is cultivated liquid, and described maggot is cultivated liquid by 500-3000g larva of a silkworm with batrytis powder, 10-80g yeast extracting powder, 0.01-0.1gNaCl, 0-1.0gKCl, 0-0.8gMgCl
2, 0.01-0.08gCaCl
2, 0-0.5gMgSO
4, 20-100gNa
2HPO
4, 0-5.0gK
2SO
4, 0-5.0g urea mixes and add the 10-15L pure water to form, add and above-mentionedly contain young maggot culture fluid and cultivate, cultivate parameter: pH=5.5-8, mixing speed 1-30r/min, temperature 25-55 ℃, throughput 5.0-20L/min, time 72-168h, larva of a silkworm with batrytis maggot becomes milk yellow by milky, and cultivating process is completed;
(7) will cultivate liquid and the larva of a silkworm with batrytis maggot of cultivating and put into seperator and carry out worm liquid and separate, with pure water washing larva of a silkworm with batrytis maggot, and use heat water-spraying's deactivation;
(8) larva of a silkworm with batrytis maggot of deactivation is dry, gets finished product;
(9) maggot packing.
Larva of a silkworm with batrytis maggot is light green color, contains protein 58-61%, fatty 3.7-4.1%, ash content 5-6%, carbohydrate 11-12% after drying.The mode that the present invention changes by biology, in growing process, the training of stiff an insect which destroys mulberry leaves worm eliminated anaphylactogen and the toxin of stiff silkworm, therefore increased the range of application of stiff silkworm, stiff an insect which destroys mulberry leaves worm has advantages of definite ingredients, nonpoisonous and tasteless as a kind of new Chinese medicine material simultaneously, and more space will be provided for the purposes of stiff silkworm.This technical process is easy to control, and saves the energy, suitability for industrialized production.
Embodiment
Below in conjunction with embodiment, the present invention is described further.
Embodiment 1:
(1) the 1000g larva of a silkworm with batrytis is crushed to 0.5-0.05mm with cracker with 250-1250r/min, and with 0.2-0.05mm aperture sieve screening, collects powder material;
(2) powder material adds the 1-50L pure water thing that stirs into a paste;
(3) pastel is at 80 to 120 ℃ of sterilization 10-30min, then be cooled to 30 ℃ standby;
(4) get the little rice bran of 100g, 0.2-0.5g larva of a silkworm with batrytis pastel, add Chinese medicine 10-20g banksia rose powder and 1.0-5.0g radix aconiti agrestis powder to mix, spray the 10-50ml pure water, keep 30 to 55 ℃ of temperature, time 48-240h, bear worm's ovum;
(5) sift out worm's ovum with aperture 0.1-2.0mm sieve standby;
(6) get 10-200g peptone, 1-5g larva of a silkworm with batrytis pastel, 5-10g yeast extracting powder, 2-10g Chinese medicine banksia rose powder, 2-10gNaCl, add the 0.5-1.0L pure water and be mixed into young maggot culture fluid, be sub-packed in 16-20 conical flask, 80-120 ℃ of sterilization 10-30min, be cooled to 20-40 ℃, be respectively charged into the worm's ovum that 0.2-1.0g cultivates previously, shaking table 1-100r/min, cultivate 12-48h, generate young maggot;
(7) liquid is cultivated in preparation, cultivates in liquid to add 500-3000g larva of a silkworm with batrytis powder, 10-80g yeast extracting powder, 0.1-1.0gKCl, 0.01-0.1gNaCl, 20-100gNa
2HPO
4, 0.01-0.05gCaCl
2, 0.1-0.5gMgSO
4, stir and add the 10-15L pure water to become the maggot culture fluid, heat 80-100 ℃, time 10-60min, be cooled to 25-35 ℃, add the glucose 50-100g of independent 100-120 ℃ of sterilization 10-20min, add above-mentionedly to contain young maggot culture fluid 1500-2500ml and cultivate; Cultivate larva of a silkworm with batrytis maggot and become milk yellow by milky, cultivating process is completed;
Cultivate parameter: pH=5.5-7, mixing speed 1-20r/min, temperature 25-55 ℃, throughput 7.0-20L/min, time 72-168h;
(8) will cultivate liquid and the larva of a silkworm with batrytis maggot cultivated is put into together seperator and separates with rotating speed 550-1250r/min worm liquid;
(9) with 50-100L pure water washing larva of a silkworm with batrytis maggot 2-3 time, and with 80-100 ℃ of heat water-spraying's deactivation 1-10min;
(10) larva of a silkworm with batrytis maggot of deactivation is dried to moisture content 5-8% and obtains finished product to 30 ℃, vacuum 100-500kPa, time 5-24h in temperature-30 with vacuum freeze drier;
(11) packing.
Embodiment 2:
(1) the 1000g larva of a silkworm with batrytis is crushed to 0.05-0.5mm and with 0.05-0.2mm aperture sieve screening, collects powder material with 250-1250r/min with cracker;
(2) powder material adds the 10-50L pure water thing that stirs into a paste;
(3) pastel 80-120 ℃ of sterilization 10-30min then be cooled to 25-30 ℃ standby;
(4) get 100g wheat bran, 30-50g larva of a silkworm with batrytis pastel, add Chinese medicine wrinkled giant hyssop 2-8g, rhizoma atractylodis 2-5g to mix, spray the 10-50ml pure water, keep 25-55 ℃ of temperature, time 48-240h generates worm's ovum;
(5) sift out worm's ovum with aperture 0.1-2.0mm sieve standby;
(6) get 50-100g larva of a silkworm with batrytis pastel, 5-10g yeast extracting powder, 2-10g Chinese medicine wrinkled giant hyssop, 2-5g Chinese medicine rhizoma atractylodis, 2-10gKCl, add the 0.5-1.0L pure water to be mixed into young maggot culture fluid, be sub-packed in 16-20 conical flask, 80-120 ℃ of sterilization 10-30min, be cooled to 20-40 ℃, every bottle is respectively charged into the worm's ovum that 0.2-1.0g cultivates previously, 10-100 time/min of shaking table, cultivate 18-48h, generate young maggot;
(7) add 1500-2000g larva of a silkworm with batrytis powder in the 10-15L deionized water, 20-80g yeast extracting powder, 2.0-5.0gK
2SO
4, 0.01-0.05gNaCl, 20-100gNa
2HPO
4, 0.01-0.08gCaCl
2, 0.1-0.8gMgCl
2, stirring becomes maggot and cultivates liquid, processes culture fluid 3-5min with 10000-15000 Gauss magnetic induction, processes to add for 3-5 time to contain young maggot culture fluid 1500-2500ml and cultivate;
Cultivate parameter: pH=5.5-8, mixing speed 1-30r/min, temperature 25-35 ℃, throughput 5.0-20L/min, time 72-168h;
Cultivate larva of a silkworm with batrytis maggot and become milk yellow by milky, cultivating process is completed;
(8) will cultivate liquid puts into together seperator with the larva of a silkworm with batrytis maggot of cultivating and separates with rotating speed 550-1250r/min worm liquid;
(9) with 50-100L pure water washing larva of a silkworm with batrytis maggot 2-3 time, and with 80-100 ℃ of heat water-spraying's deactivation 2.0-10min;
(10) larva of a silkworm with batrytis maggot of deactivation is dried to moisture content 5-8% and obtains finished product at temperature-30-30 ℃, vacuum 100-500kPa, time 5-24h with vacuum freeze drier;
(11) maggot packing.
Embodiment 3:
(1) the 1000g larva of a silkworm with batrytis is crushed to 0.05-0.5mm and with 0.05-0.2mm aperture sieve screening, collects powder material with 250-1250r/min with cracker;
(2) powder material adds the 1-50L pure water thing that stirs into a paste;
(3) pastel is at 80-120 ℃ of sterilization 10-30min, then be cooled to 25-30 ℃ standby;
(4) get the 100g buckwheat bran and add larva of a silkworm with batrytis pastel 5-10g, Chinese medicine Ground Cloves 5-10g, rhizoma atractylodis powder 2-5g to mix, spray the 10-50ml pure water, keep 25-55 ℃ of temperature, time 48-240h generates worm's ovum;
(5) sift out worm's ovum with aperture 0.1-2.0mm sieve standby;
(6) get 50-100g soybean protein isolate, 5-10g yeast extracting powder, 20-50g larva of a silkworm with batrytis pastel, 15-20g Chinese medicine Ground Cloves, 20-30g rhizoma atractylodis powder, 2-10NaCl, add the 0.5-1.0L pure water to be mixed into young maggot culture fluid, be sub-packed in 16-20 conical flask, 80-120 ℃ of sterilization 10-30min, be cooled to 20-40 ℃, every bottle is respectively charged into the worm's ovum that 0.2-1.0g cultivates previously, 10-100 time/min of shaking table, cultivate 12-48h, generate young maggot;
(7) get 1500-2000g larva of a silkworm with batrytis powder, 10-80g yeast extracting powder, 2.0-5.0g urea, 0.01-0.05gNaCl, 20-100gNaH
2PO
4, 0.01-0.08gCaCl
2, 0.1-0.8gMgCl
2Stir and add the 10-15L pure water to become maggot and cultivate liquid, with ultrasonic 2450-5000kHz, 1kw-10kw, processing culture fluid 10-30min, then add and above-mentionedly contain young maggot culture fluid 1500-2500ml and cultivate, cultivate larva of a silkworm with batrytis maggot and become milk yellow by milky, cultivating process is completed;
Cultivate parameter: pH=5.5-8, mixing speed 1-30r/min, temperature 25-35 ℃, throughput 5.0-20L/min, time 72-168h;
(8) will cultivate liquid and the larva of a silkworm with batrytis maggot cultivated is put into seperator together, separate with rotating speed 550-1250r/min worm liquid;
(9) with 50-100L pure water washing larva of a silkworm with batrytis maggot 2-3 time, and with 80-100 ℃ of heat water-spraying's deactivation 2.0-10min;
(10) larva of a silkworm with batrytis maggot of deactivation is dried to moisture content 5-8% and obtains finished product at temperature-30-30 ℃, vacuum 100-500kPa, time 5-24h with vacuum freeze drier;
(11) maggot packing.
Embodiment 4:
(1) the 1000g larva of a silkworm with batrytis is crushed to 0.05-0.5mm and with 0.05-0.2mm aperture sieve screening, collects powder material with 250-1250r/min with cracker;
(2) powder material adds the 1-50L pure water thing that stirs into a paste;
(3) pastel is at 80-120 ℃ of sterilization 10-30min, then be cooled to 25-30 ℃ standby;
(4) get 100g agar and add larva of a silkworm with batrytis pastel 5-10g, Chinese medicine wrinkled giant hyssop powder 2-8g, banksia rose powder 2-6g to mix, spray the 10-50ml pure water, keep 25-55 ℃ of temperature, time 48-240h generates worm's ovum;
(5) sift out worm's ovum with aperture 0.1-2.0mm sieve standby;
(6) get 50-100g peptone, 5-10g yeast extracting powder, 20-50g larva of a silkworm with batrytis pastel, 2-10gNaCl, add the 0.5-1.0L pure water and be mixed into young maggot culture fluid, be sub-packed in 16-20 conical flask, 80-120 ℃ of sterilization 10-30min, be cooled to 20-40 ℃, be respectively charged into the worm's ovum that 0.2-1.0g cultivates previously, 1-10 time/min of shaking table, cultivate 12-48h, generate young maggot;
(7) liquid is cultivated in preparation, cultivates in liquid to add 500-3000g larva of a silkworm with batrytis powder, 10-80g yeast extracting powder, 0.1-1.0gKCl, 0.01-0.1gNaCl, 20-100gNaH
2PO
4, 0.01-0.05gCaCl
2, 0.1-0.5gMgSO
4Stir and add the 10-15L pure water to become maggot and cultivate liquid, process with microwave frequency 2450-915MHz, power 0.5-2.5kw and cultivate liquid 2-10min, add to contain young maggot culture fluid 1500-2500ml and cultivate,
Cultivate parameter: pH=5.5-8, mixing speed 1-30r/min, temperature 25-35 ℃, throughput 5-20L/min, time 72-168h;
Cultivation larva of a silkworm with batrytis maggot becomes the milk yellow cultivating process by milky and completes;
(8) will cultivate liquid and the larva of a silkworm with batrytis maggot cultivated is put into together seperator and separates with rotating speed 550-1250r/min worm liquid;
(9) with 50-100L pure water washing larva of a silkworm with batrytis maggot 2-3 time, and with 80-100 ℃ of heat water-spraying's deactivation 2.0-10min;
(10) larva of a silkworm with batrytis maggot of deactivation is dried to larva of a silkworm with batrytis maggot moisture content 5-8% and gets finished product at temperature-30-30 ℃, vacuum 100-500kpa, time 5-24h with vacuum freeze drier;
(11) maggot packing.
Claims (7)
1. the technique of artificial culture larva of a silkworm with batrytis maggot, is characterized in that described technique comprises the steps:
(1) larva of a silkworm with batrytis be crushed to Powdered,
(2) be mixed into larva of a silkworm with batrytis pastel with water;
(3) larva of a silkworm with batrytis pastel high temperature sterilization;
(4) cereal bran and larva of a silkworm with batrytis pastel are processed with Chinese medicine and are formed egg cultivation liquid, described egg cultivation liquid is mixed by 100g cereal bran, larva of a silkworm with batrytis pastel 0.2-50g, Ground Cloves 0-10g, rhizoma atractylodis powder 0-5g, wrinkled giant hyssop 0-8g, 0-20g banksia rose powder and 0-5.0g radix aconiti agrestis powder, spray the 10-50ml pure water, keep 30-55 ℃ of temperature, time 48-240h, generate worm's ovum;
(5) young maggot training is grown: prepare young maggot culture fluid, described young maggot culture fluid is mixed by 1-100g larva of a silkworm with batrytis pastel, 0-200g peptone, 5-10g yeast extracting powder, 0-10g banksia rose powder, 0-10g wrinkled giant hyssop, 0-30g rhizoma atractylodis powder, 0-20g Chinese medicine Ground Cloves, 2-10gNaCl or KCl, 0-100g soybean protein isolate, 0.5-1.0L pure water, be sub-packed in conical flask, be respectively charged into the worm's ovum that the front training is grown after sterilization, shaking table 1-100r/min, cultivate 12-48h, generate the culture fluid that contains larva of a silkworm with batrytis children maggot;
(6) maggot is cultivated: the preparation maggot is cultivated liquid, and described maggot is cultivated liquid by 500-3000g larva of a silkworm with batrytis powder, 10-80g yeast extracting powder, 0.01-0.1gNaCl, 0-1.0gKCl, 0-0.8gMgCl
2, 0.01-0.08gCaCl
2, 0-0.5gMgSO
4, 20-100gNa
2HPO
4, 0-5.0gK
2SO
4, 0-5.0g urea mixes and add the 10-15L pure water, add and contain young maggot culture fluid and cultivate, cultivate parameter: pH=5.5-8, mixing speed 1-30r/min, temperature 25-55 ℃, throughput 5.0-20L/min, time 72-168h, larva of a silkworm with batrytis maggot becomes milk yellow by milky, and cultivating process is completed;
(7) will cultivate liquid and the larva of a silkworm with batrytis maggot of cultivating and put into seperator and carry out worm liquid and separate, with pure water washing larva of a silkworm with batrytis maggot, and use heat water-spraying's deactivation;
(8) larva of a silkworm with batrytis maggot of deactivation is dry, gets finished product.
2. the technique of artificial culture larva of a silkworm with batrytis maggot according to claim 1, is characterized in that described cereal bran is little rice bran, wheat bran or buckwheat bran.
3. the technique of artificial culture larva of a silkworm with batrytis maggot according to claim 1, is characterized in that described egg cultivation liquid is mixed by agar 100g, larva of a silkworm with batrytis pastel 5-10g, wrinkled giant hyssop powder 2-8g, banksia rose powder 2-6g.
4. the technique of artificial culture larva of a silkworm with batrytis maggot according to claim 1, is characterized in that in described step (3), and pastel is at 80 to 120 ℃ of sterilization 10-30min, then be cooled to 30 ℃ standby.
5. the technique of artificial culture larva of a silkworm with batrytis maggot according to claim 1, is characterized in that in described step (7), the seperator rotating speed is 550-1250r/min.
6. the technique of artificial culture larva of a silkworm with batrytis maggot according to claim 1, is characterized in that in described step (7), with pure water washing larva of a silkworm with batrytis maggot 2-3 time, and with 80-100 ℃ of heat water-spraying's deactivation 1-10min.
7. the technique of artificial culture larva of a silkworm with batrytis maggot according to claim 1, is characterized in that in described step (8), and the larva of a silkworm with batrytis maggot of deactivation is dried to moisture content 5-8% and obtains finished product at temperature-30-30 ℃, vacuum 100-500kPa, time 5-24h.
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| RU2659830C1 (en) * | 2016-09-05 | 2018-07-04 | федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный университет" (ФГБОУ ВО "ВГУ") | Multi-component substrate for storage and transportation of agents of biological protection of plants and also other small animals |
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| CN1056956C (en) * | 1998-01-15 | 2000-10-04 | 黄明礼 | Method for artificial propagation of toad maggot |
| CN1149013C (en) * | 2000-09-22 | 2004-05-12 | 王欧 | Method and equipment for large-scale breeding fly maggots |
| CN1324957C (en) * | 2004-05-10 | 2007-07-11 | 孔繁伟 | Method for cultivating toad evildoers using traditional Chinese medicine |
| CN102273430A (en) * | 2010-06-09 | 2011-12-14 | 张春燕 | Culturing method of maggot containing toad toxication component and treating function of preparation of maggot |
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