CN102793726A - Process for manually culturing silkworm larva maggots - Google Patents
Process for manually culturing silkworm larva maggots Download PDFInfo
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- CN102793726A CN102793726A CN2012103155931A CN201210315593A CN102793726A CN 102793726 A CN102793726 A CN 102793726A CN 2012103155931 A CN2012103155931 A CN 2012103155931A CN 201210315593 A CN201210315593 A CN 201210315593A CN 102793726 A CN102793726 A CN 102793726A
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Abstract
The invention discloses a process for manually culturing silkworm larva maggots, which relates to a process for manually culturing insects. Specific to the limitation on the use of silkworm larvae and the influence of processing, a process for manually culturing silkworm larva maggots comprises the following steps of: (1) smashing silkworm larvae into powder; (2) mixing with water and stirring into paste; (3) sterilizing the paste at a high temperature; (4) treating grain bran with Chinese medicines to generate maggot eggs; (5) culturing young maggots; (6) culturing maggots; (7) separating maggots, cleaning and inactivating; (8) drying maggots; and (9) packing maggots. A biotransformation way is adopted, and allergens and toxins of stiff silkworms are eliminated in the culturing process of stiff silkworm maggots, so that the application range of the stiff silkworms is expanded; and the stiff silkworm maggots serving as a novel Chinese medicinal raw material has the advantages of definite composition, nontoxicity and tastelessness, so that more space is provided for the application of stiff silkworms. A technical process is easy to control, energy is saved, and the process is suitable for industrial production.
Description
Technical field
The present invention relates to the technology of artificial culture insecticide, particularly the technology of artificial culture Bombyx Batryticatus maggot.
Background technology
Bombyx Batryticatus is a kind of traditional Chinese medicines material, and Bombyx Batryticatus is that Bombycidae animal bombyx mori larva infects muscardine and ossified Scorpio.Bombyx Batryticatus contains 28 kinds of trace element such as protein, ammonium oxalate, 17 seed amino acids, calcium, magnesium and abnormal active hormones, ecdysone.The Bombyx Batryticatus thalline contains soft beauvericin, contains compositions such as multiple ring acetal acid peptide, fatty acid amide lipoid.
CN101810689A discloses a kind of prescription of batryticated silkworm processed by gall bladder, comprises Bombyx Batryticatus, Rhizoma Coptidis, cattle gall bladder, Periostracum Cicadae composition; CN102153619A discloses a kind of small-molecular weight glycopeptide that from Bombyx Batryticatus, extracts, its extraction separation method and purposes, the Chinese crude drug Bombyx Batryticatus pulverize the back through extraction, ultrafiltration, concentrate and the ethanol precipitation extraction separation gets the effective ingredient glycopeptide; CN101062067 discloses muscardine silkworm skin recovery powder, is made up of according to a certain ratio Bombyx Batryticatus, the Radix Angelicae Dahuricae, Scolopendra, Scorpio.
ZL20041003742.4 discloses and has adopted Chinese medicine to cultivate the method for Bufo siccus maggot, adopts this method suitability for industrialized production Bufo siccus maggot in batches.
Bombyx Batryticatus is owing to be that the bombyx mori larva infects muscardine and ossified Scorpio; Muscardine has an immense impact on to it; Allergy is a kind of important behaviour, so Bombyx Batryticatus often needs methods such as parch, parched with bran, processed with Rhizoma Zingiberis Recens, processed with wine, liquorice beverage system to handle when using, and all needs high-temperature heating treatment; Effective medicinal components is exerted an influence, reduced drug effect.
Summary of the invention
To the limitation of using Bombyx Batryticatus and the influence of the process of preparing Chinese medicine, the present invention provides the technology of a kind of artificial culture Bombyx Batryticatus maggot.
The objective of the invention is to solve through following technical scheme: (1) Bombyx Batryticatus is crushed to Powdered; (2) be mixed into the Bombyx Batryticatus pastel with water; (3) Bombyx Batryticatus pastel high temperature sterilization; (4) corn bran and Bombyx Batryticatus pastel are handled with Chinese medicine, and generate the maggot ovum; (5) the young maggot culture fluid of preparation carries out the training of young maggot and grows; (6) preparation maggot culture fluid carries out the maggot cultivation; (7) maggot separation, cleaning, deactivation; (8) maggot is dry; (9) maggot packing.Concrete steps are following:
(1) Bombyx Batryticatus be crushed to Powdered,
(2) be mixed into the Bombyx Batryticatus pastel with water;
(3) Bombyx Batryticatus pastel high temperature sterilization;
(4) corn furfur and Bombyx Batryticatus pastel are handled with Chinese medicine and are formed the worm's ovum culture fluid; Said worm's ovum culture fluid is mixed by 100g corn furfur, Bombyx Batryticatus pastel 0.2-50g, Flos Caryophylli powder 0-10g, Rhizoma Atractylodis powder 0-5g, Herba Pogostemonis 0-8g, 0-20g Radix Aucklandiae powder and 0-5.0g Radix Aconiti Kusnezoffii powder; Keep 30-55 ℃ of temperature, time 48-240h, generate worm's ovum;
(5) young maggot training is grown: prepare young maggot culture fluid; Said young maggot culture fluid is mixed by 1-100g Bombyx Batryticatus pastel, 0-200g peptone, 5-10g yeast extracting powder, 0-10g Radix Aucklandiae powder, 0-10g Herba Pogostemonis, 0-30g Rhizoma Atractylodis powder, 0-20g Chinese medicine Flos Caryophylli powder, 2-10gNaCl or KCl, 0-100g soybean protein isolate, 0.5-1.0L pure water; Be sub-packed in the conical flask; Be respectively charged into the worm's ovum that the front training is grown after the sterilization; Shaking table 1-100r/min cultivates 12-48h, generates the culture fluid that contains Bombyx Batryticatus children maggot;
(6) maggot is cultivated: the preparation maggot is cultivated liquid, and said maggot is cultivated liquid by 500-3000g Bombyx Batryticatus powder, 10-80g yeast extracting powder, 0.01-0.1gNaCl, 0-1.0gKCl, 0-0.8gMgCl
2, 0.01-0.08gCaCl
2, 0-0.5gMgSO
4, 20-100gNa
2HPO
4, 0-5.0gK
2SO
4, 0-5.0g carbamide mix homogeneously and add the 10-15L pure water and form, add and above-mentionedly contain young maggot culture fluid and cultivate, cultivate parameter: pH=5.5-8; Mixing speed 1-30r/min; Temperature 25-55 ℃, ventilation 5.0-20L/min, time 72-168h; The Bombyx Batryticatus maggot becomes milk yellow by milky, and cultivating process is accomplished;
(7) will cultivate liquid and the Bombyx Batryticatus maggot of cultivating and put into seperator and carry out worm liquid and separate,, and use heat water-spraying's deactivation with pure water washing Bombyx Batryticatus maggot;
(8) the Bombyx Batryticatus maggot of deactivation is dry, gets finished product;
(9) maggot packing.
The Bombyx Batryticatus maggot is a light green color, contains protein 58-61%, fatty 3.7-4.1%, ash 5-6%, carbohydrate 11-12% after the drying.The present invention is through the mode of biotransformation; Grow anaphylactogen and the toxin of having eliminated Bombyx Batryticatus in the process in the training of Bombyx Batryticatus maggot; Therefore increased the range of application of Bombyx Batryticatus; Simultaneously the Bombyx Batryticatus maggot has definite ingredients, nonpoisonous and tasteless advantage as a kind of new raw material of Chinese medicine, will more space be provided for the purposes of Bombyx Batryticatus.This technical process is easy to control, saves the energy, suitability for industrialized production.
The specific embodiment
Below in conjunction with embodiment the present invention is done and to further describe.
Embodiment 1:
(1) the 1000g Bombyx Batryticatus is crushed to 0.5-0.05mm with pulverizer with 250-1250r/min, and with the screening of 0.2-0.05mm aperture sieve, collects powder material;
(2) powder material adds the 1-50L pure water thing that stirs into a paste;
(3) pastel is at 80 to 120 ℃ of antibacterial 10-30min, be cooled to then 30 ℃ subsequent use;
(4) get 100g Testa oryzae, 0.2-0.5g Bombyx Batryticatus pastel, add Chinese medicine 10-20g Radix Aucklandiae powder and 1.0-5.0g Radix Aconiti Kusnezoffii powder mix homogeneously, spray the 10-50ml pure water, keep 30 to 55 ℃ of temperature, time 48-240h, bear worm's ovum;
(5) it is subsequent use to sift out worm's ovum with aperture 0.1-2.0mm sieve;
(6) get 10-200g peptone, 1-5g Bombyx Batryticatus pastel, 5-10g yeast extracting powder, 2-10g Chinese medicine Radix Aucklandiae powder, 2-10gNaCl, add the 0.5-1.0L pure water and be mixed into young maggot culture fluid, be sub-packed in 16-20 the conical flask; 80-120 ℃ of sterilization 10-30min; Be cooled to 20-40 ℃, be respectively charged into the worm's ovum that cultivate the 0.2-1.0g front, shaking table 1-100r/min; Cultivate 12-48h, generate young maggot;
(7) liquid is cultivated in preparation, cultivates to add 500-3000g Bombyx Batryticatus powder, 10-80g yeast extracting powder, 0.1-1.0gKCl, 0.01-0.1gNaCl, 20-100gNa in the liquid
2HPO
4, 0.01-0.05gCaCl
2, 0.1-0.5gMgSO
4, stirring and adding the 10-15L pure water becomes the maggot culture fluid, heats 80-100 ℃; Time 10-60min; Be cooled to 25-35 ℃, add the glucose 50-100g of independent 100-120 ℃ of sterilization 10-20min, add and above-mentionedly contain young maggot culture fluid 1500-2500ml and cultivate; Cultivate the Bombyx Batryticatus maggot and become milk yellow by milky, cultivating process is accomplished;
Cultivate parameter: pH=5.5-7, mixing speed 1-20r/min, temperature 25-55 ℃, ventilation 7.0-20L/min, time 72-168h;
(8) will cultivate liquid and the Bombyx Batryticatus maggot cultivated is put into seperator together and separates with rotating speed 550-1250r/min worm liquid;
(9) wash the Bombyx Batryticatus maggot 2-3 time with the 50-100L pure water, and with 80-100 ℃ of heat water-spraying's deactivation 1-10min;
(10) the Bombyx Batryticatus maggot of deactivation is dried at temperature-30 to 30 ℃, vacuum 100-500kPa, time 5-24h with vacuum freeze drier and contains moisture 5-8% and obtain finished product;
(11) packing.
Embodiment 2:
(1) the 1000g Bombyx Batryticatus is crushed to 0.05-0.5mm and with the screening of 0.05-0.2mm aperture sieve, collects powder material with 250-1250r/min with pulverizer;
(2) powder material adds the 10-50L pure water thing that stirs into a paste;
(3) pastel 80-120 ℃ of antibacterial 10-30min be cooled to then 25-30 ℃ subsequent use;
(4) get 100g Testa Tritici, 30-50g Bombyx Batryticatus pastel, add Chinese medicine Herba Pogostemonis 2-8g, Rhizoma Atractylodis 2-5g mix homogeneously, spray the 10-50ml pure water, keep 25-55 ℃ of temperature, time 48-240h generates worm's ovum;
(5) it is subsequent use to sift out worm's ovum with aperture 0.1-2.0mm sieve;
(6) get 50-100g Bombyx Batryticatus pastel, 5-10g yeast extracting powder, 2-10g Chinese medicine Herba Pogostemonis, 2-5g Chinese medicine Rhizoma Atractylodis, 2-10gKCl, add the 0.5-1.0L pure water and be mixed into young maggot culture fluid, be sub-packed in 16-20 the conical flask; 80-120 ℃ of sterilization 10-30min; Be cooled to 20-40 ℃, every bottle is respectively charged into the worm's ovum that cultivate the 0.2-1.0g front, 10-100 time/min of shaking table; Cultivate 18-48h, generate young maggot;
(7) add 1500-2000g Bombyx Batryticatus powder, 20-80g yeast extracting powder, 2.0-5.0gK in the 10-15L deionized water
2SO
4, 0.01-0.05gNaCl, 20-100gNa
2HPO
4, 0.01-0.08gCaCl
2, 0.1-0.8gMgCl
2, stirring becomes maggot and cultivates liquid, handles culture fluid 3-5min with 10000-15000 Gauss magnetic induction, handles 3-5 adding and contains young maggot culture fluid 1500-2500ml and cultivate;
Cultivate parameter: pH=5.5-8, mixing speed 1-30r/min, temperature 25-35 ℃, ventilation 5.0-20L/min, time 72-168h;
Cultivate the Bombyx Batryticatus maggot and become milk yellow by milky, cultivating process is accomplished;
(8) will cultivate liquid puts into seperator together with the Bombyx Batryticatus maggot of cultivating and separates with rotating speed 550-1250r/min worm liquid;
(9) wash the Bombyx Batryticatus maggot 2-3 time with the 50-100L pure water, and with 80-100 ℃ of heat water-spraying's deactivation 2.0-10min;
(10) the Bombyx Batryticatus maggot of deactivation is dried at temperature-30-30 ℃, vacuum 100-500kPa, time 5-24h with vacuum freeze drier and contains moisture 5-8% and obtain finished product;
(11) maggot packing.
Embodiment 3:
(1) the 1000g Bombyx Batryticatus is crushed to 0.05-0.5mm and with the screening of 0.05-0.2mm aperture sieve, collects powder material with 250-1250r/min with pulverizer;
(2) powder material adds the 1-50L pure water thing that stirs into a paste;
(3) pastel is at 80-120 ℃ of antibacterial 10-30min, be cooled to then 25-30 ℃ subsequent use;
(4) get the 100g buckwheat bran and add Bombyx Batryticatus pastel 5-10g, Chinese medicine Flos Caryophylli powder 5-10g, Rhizoma Atractylodis powder 2-5g mix homogeneously, spray the 10-50ml pure water, keep 25-55 ℃ of temperature, time 48-240h generates worm's ovum;
(5) it is subsequent use to sift out worm's ovum with aperture 0.1-2.0mm sieve;
(6) get 50-100g soybean protein isolate, 5-10g yeast extracting powder, 20-50g Bombyx Batryticatus pastel, 15-20g Chinese medicine Flos Caryophylli powder, 20-30g Rhizoma Atractylodis powder, 2-10NaCl, add the 0.5-1.0L pure water and be mixed into young maggot culture fluid, be sub-packed in 16-20 the conical flask; 80-120 ℃ of sterilization 10-30min; Be cooled to 20-40 ℃, every bottle is respectively charged into the worm's ovum that cultivate the 0.2-1.0g front, 10-100 time/min of shaking table; Cultivate 12-48h, generate young maggot;
(7) get 1500-2000g Bombyx Batryticatus powder, 10-80g yeast extracting powder, 2.0-5.0g carbamide, 0.01-0.05gNaCl, 20-100gNaH
2PO
4, 0.01-0.08gCaCl
2, 0.1-0.8gMgCl
2Stir and add the 10-15L pure water and become maggot cultivation liquid; With ultrasound wave 2450-5000kHz, 1kw-10kw, processing culture fluid 10-30min; Add then and above-mentionedly contain young maggot culture fluid 1500-2500ml and cultivate; Cultivate the Bombyx Batryticatus maggot and become milk yellow by milky, cultivating process is accomplished;
Cultivate parameter: pH=5.5-8, mixing speed 1-30r/min, temperature 25-35 ℃, ventilation 5.0-20L/min, time 72-168h;
(8) will cultivate liquid and the Bombyx Batryticatus maggot cultivated is put into seperator together, separate with rotating speed 550-1250r/min worm liquid;
(9) wash the Bombyx Batryticatus maggot 2-3 time with the 50-100L pure water, and with 80-100 ℃ of heat water-spraying's deactivation 2.0-10min;
(10) the Bombyx Batryticatus maggot of deactivation is dried at temperature-30-30 ℃, vacuum 100-500kPa, time 5-24h with vacuum freeze drier and contains moisture 5-8% and obtain finished product;
(11) maggot packing.
Embodiment 4:
(1) the 1000g Bombyx Batryticatus is crushed to 0.05-0.5mm and with the screening of 0.05-0.2mm aperture sieve, collects powder material with 250-1250r/min with pulverizer;
(2) powder material adds the 1-50L pure water thing that stirs into a paste;
(3) pastel is at 80-120 ℃ of antibacterial 10-30min, be cooled to then 25-30 ℃ subsequent use;
(4) get 100g agar and add Bombyx Batryticatus pastel 5-10g, Chinese medicine Herba Pogostemonis powder 2-8g, Radix Aucklandiae powder 2-6g mix homogeneously, spray the 10-50ml pure water, keep 25-55 ℃ of temperature, time 48-240h generates worm's ovum;
(5) it is subsequent use to sift out worm's ovum with aperture 0.1-2.0mm sieve;
(6) get 50-100g peptone, 5-10g yeast extracting powder, 20-50g Bombyx Batryticatus pastel, 2-10gNaCl, add the 0.5-1.0L pure water and be mixed into young maggot culture fluid, be sub-packed in 16-20 the conical flask; 80-120 ℃ of sterilization 10-30min; Be cooled to 20-40 ℃, be respectively charged into the worm's ovum that cultivate the 0.2-1.0g front, 1-10 time/min of shaking table; Cultivate 12-48h, generate young maggot;
(7) liquid is cultivated in preparation, cultivates to add 500-3000g Bombyx Batryticatus powder, 10-80g yeast extracting powder, 0.1-1.0gKCl, 0.01-0.1gNaCl, 20-100gNaH in the liquid
2PO
4, 0.01-0.05gCaCl
2, 0.1-0.5gMgSO
4Stirring and adding the 10-15L pure water becomes maggot cultivation liquid, handles with microwave frequency 2450-915MHz, power 0.5-2.5kw and cultivates liquid 2-10min, and adding contains young maggot culture fluid 1500-2500ml cultivates,
Cultivate parameter: pH=5.5-8, mixing speed 1-30r/min, temperature 25-35 ℃, ventilation 5-20L/min, time 72-168h;
Cultivate the Bombyx Batryticatus maggot and become the completion of milk yellow cultivating process by milky;
(8) will cultivate liquid and the Bombyx Batryticatus maggot cultivated is put into seperator together and separates with rotating speed 550-1250r/min worm liquid;
(9) wash the Bombyx Batryticatus maggot 2-3 time with the 50-100L pure water, and with 80-100 ℃ of heat water-spraying's deactivation 2.0-10min;
(10) the Bombyx Batryticatus maggot of deactivation is dried to the Bombyx Batryticatus maggot with vacuum freeze drier at temperature-30-30 ℃, vacuum 100-500kpa, time 5-24h and contains moisture 5-8% and get finished product;
(11) maggot packing.
Claims (7)
1. the technology of artificial culture Bombyx Batryticatus maggot is characterized in that said technology comprises the steps:
(1) Bombyx Batryticatus be crushed to Powdered,
(2) be mixed into the Bombyx Batryticatus pastel with water;
(3) Bombyx Batryticatus pastel high temperature sterilization;
(4) corn furfur and Bombyx Batryticatus pastel are handled with Chinese medicine and are formed the worm's ovum culture fluid; Said worm's ovum culture fluid is mixed by 100g corn furfur, Bombyx Batryticatus pastel 0.2-50g, Flos Caryophylli powder 0-10g, Rhizoma Atractylodis powder 0-5g, Herba Pogostemonis 0-8g, 0-20g Radix Aucklandiae powder and 0-5.0g Radix Aconiti Kusnezoffii powder; Keep 30-55 ℃ of temperature, time 48-240h, generate worm's ovum;
(5) young maggot training is grown: prepare young maggot culture fluid; Said young maggot culture fluid is mixed by 1-100g Bombyx Batryticatus pastel, 0-200g peptone, 5-10g yeast extracting powder, 0-10g Radix Aucklandiae powder, 0-10g Herba Pogostemonis, 0-30g Rhizoma Atractylodis powder, 0-20g Chinese medicine Flos Caryophylli powder, 2-10gNaCl or KCl, 0-100g soybean protein isolate, 0.5-1.0L pure water; Be sub-packed in the conical flask; Be respectively charged into the worm's ovum that the front training is grown after the sterilization; Shaking table 1-100r/min cultivates 12-48h, generates the culture fluid that contains Bombyx Batryticatus children maggot;
(6) maggot is cultivated: the preparation maggot is cultivated liquid, and said maggot is cultivated liquid by 500-3000g Bombyx Batryticatus powder, 10-80g yeast extracting powder, 0.01-0.1gNaCl, 0-1.0gKCl, 0-0.8gMgCl
2, 0.01-0.08gCaCl
2, 0-0.5gMgSO
4, 20-100gNa
2HPO
4, 0-5.0gK
2SO
4, 0-5.0g carbamide mixes and add the 10-15L pure water, adds to contain young maggot culture fluid and cultivates, and cultivates parameter: pH=5.5-8; Mixing speed 1-30r/min; Temperature 25-55 ℃, ventilation 5.0-20L/min, time 72-168h; The Bombyx Batryticatus maggot becomes milk yellow by milky, and cultivating process is accomplished;
(7) will cultivate liquid and the Bombyx Batryticatus maggot of cultivating and put into seperator and carry out worm liquid and separate,, and use heat water-spraying's deactivation with pure water washing Bombyx Batryticatus maggot;
(8) the Bombyx Batryticatus maggot of deactivation is dry, gets finished product.
2. the technology of artificial culture Bombyx Batryticatus maggot according to claim 1 is characterized in that said corn furfur is Testa oryzae, Testa Tritici or buckwheat bran.
3. the technology of artificial culture Bombyx Batryticatus maggot according to claim 1 is characterized in that said worm's ovum culture fluid is mixed by agar 100g, Bombyx Batryticatus pastel 5-10g, Herba Pogostemonis powder 2-8g, Radix Aucklandiae powder 2-6g.
4. the technology of artificial culture Bombyx Batryticatus maggot according to claim 1 is characterized in that in the said step (3), and pastel is at 80 to 120 ℃ of antibacterial 10-30min, be cooled to then 30 ℃ subsequent use.
5. the technology of artificial culture Bombyx Batryticatus maggot according to claim 1 is characterized in that in the said step (7), the seperator rotating speed is 550-1250r/min.
6. the technology of artificial culture Bombyx Batryticatus maggot according to claim 1 is characterized in that in the said step (7), washs the Bombyx Batryticatus maggot 2-3 time with pure water, and with 80-100 ℃ of heat water-spraying's deactivation 1-10min.
7. the technology of artificial culture Bombyx Batryticatus maggot according to claim 1 is characterized in that in the said step (8), and the Bombyx Batryticatus maggot of deactivation is dried at temperature-30-30 ℃, vacuum 100-500kPa, time 5-24h and contains moisture 5-8% and obtain finished product.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104623624A (en) * | 2015-03-02 | 2015-05-20 | 哈尔滨工业大学 | Preparation method of white muscardine silkworm maggot protein and application of white muscardine silkworm maggot protein in preparing anti-breast cancer drugs |
| RU2659830C1 (en) * | 2016-09-05 | 2018-07-04 | федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный университет" (ФГБОУ ВО "ВГУ") | Multi-component substrate for storage and transportation of agents of biological protection of plants and also other small animals |
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| CN1295791A (en) * | 2000-09-22 | 2001-05-23 | 王欧 | Method and equipment for large-scale breeding fly maggots |
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| CN102273430A (en) * | 2010-06-09 | 2011-12-14 | 张春燕 | Culturing method of maggot containing toad toxication component and treating function of preparation of maggot |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1196169A (en) * | 1998-01-15 | 1998-10-21 | 黄明礼 | Method for artificial propagation of toad maggot |
| CN1295791A (en) * | 2000-09-22 | 2001-05-23 | 王欧 | Method and equipment for large-scale breeding fly maggots |
| CN1568681A (en) * | 2004-05-10 | 2005-01-26 | 孔繁伟 | Method for cultivating toad evildoers using traditional Chinese medicine |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104623624A (en) * | 2015-03-02 | 2015-05-20 | 哈尔滨工业大学 | Preparation method of white muscardine silkworm maggot protein and application of white muscardine silkworm maggot protein in preparing anti-breast cancer drugs |
| RU2659830C1 (en) * | 2016-09-05 | 2018-07-04 | федеральное государственное бюджетное образовательное учреждение высшего образования "Воронежский государственный университет" (ФГБОУ ВО "ВГУ") | Multi-component substrate for storage and transportation of agents of biological protection of plants and also other small animals |
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|---|---|
| CN102793726B (en) | 2013-11-06 |
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