CN106072095A - The preparation method of active seabuckthorn polypeptide - Google Patents
The preparation method of active seabuckthorn polypeptide Download PDFInfo
- Publication number
- CN106072095A CN106072095A CN201610379068.4A CN201610379068A CN106072095A CN 106072095 A CN106072095 A CN 106072095A CN 201610379068 A CN201610379068 A CN 201610379068A CN 106072095 A CN106072095 A CN 106072095A
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- CN
- China
- Prior art keywords
- fructus hippophae
- mixed liquor
- polypeptide
- enzymolysis
- prepares
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 235000003145 Hippophae rhamnoides Nutrition 0.000 title claims abstract description 34
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 28
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 23
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 240000000950 Hippophae rhamnoides Species 0.000 title 1
- 241000229143 Hippophae Species 0.000 claims abstract description 96
- 235000003935 Hippophae Nutrition 0.000 claims abstract description 63
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 29
- 108090000790 Enzymes Proteins 0.000 claims abstract description 18
- 102000004190 Enzymes Human genes 0.000 claims abstract description 18
- 230000002779 inactivation Effects 0.000 claims abstract description 7
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 235000013399 edible fruits Nutrition 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 11
- 230000004913 activation Effects 0.000 claims description 6
- 239000000084 colloidal system Substances 0.000 claims description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 5
- 238000004806 packaging method and process Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 239000004576 sand Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 6
- 238000001914 filtration Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 13
- 239000000047 product Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 238000009413 insulation Methods 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000006378 damage Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- XFZJEEAOWLFHDH-UHFFFAOYSA-N (2R,2'R,3R,3'R,4R)-3,3',4',5,7-Pentahydroxyflavan(48)-3,3',4',5,7-pentahydroxyflavan Natural products C=12OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C(O)C=C(O)C=1C(C1=C(O)C=C(O)C=C1O1)C(O)C1C1=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-UHFFFAOYSA-N 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- CWEZAWNPTYBADX-UHFFFAOYSA-N Procyanidin Natural products OC1C(OC2C(O)C(Oc3c2c(O)cc(O)c3C4C(O)C(Oc5cc(O)cc(O)c45)c6ccc(O)c(O)c6)c7ccc(O)c(O)c7)c8c(O)cc(O)cc8OC1c9ccc(O)c(O)c9 CWEZAWNPTYBADX-UHFFFAOYSA-N 0.000 description 1
- MOJZMWJRUKIQGL-FWCKPOPSSA-N Procyanidin C2 Natural products O[C@@H]1[C@@H](c2cc(O)c(O)cc2)Oc2c([C@H]3[C@H](O)[C@@H](c4cc(O)c(O)cc4)Oc4c3c(O)cc(O)c4)c(O)cc(O)c2[C@@H]1c1c(O)cc(O)c2c1O[C@@H]([C@H](O)C2)c1cc(O)c(O)cc1 MOJZMWJRUKIQGL-FWCKPOPSSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000001728 nano-filtration Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- HGVVOUNEGQIPMS-UHFFFAOYSA-N procyanidin Chemical compound O1C2=CC(O)=CC(O)=C2C(O)C(O)C1(C=1C=C(O)C(O)=CC=1)OC1CC2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 HGVVOUNEGQIPMS-UHFFFAOYSA-N 0.000 description 1
- 229920002414 procyanidin Polymers 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses the preparation method of a kind of active seabuckthorn polypeptide, it includes following step: (a) pulverizes preparation and include the Fructus Hippophae serosity of Fructus Hippophae sarcocarp and seed；B () prepares Sucus Chaenomelis；C () Fructus Hippophae serosity mixes with Sucus Chaenomelis, heats, is incubated enzymolysis, prepares Fructus Hippophae enzymolysis mixed liquor；D enzyme in Fructus Hippophae enzymolysis mixed liquor is inactivated by ()；E () uses ethanol to activate the Fructus Hippophae enzymolysis mixed liquor at enzyme inactivation；(f) filtration, fill, sterilizing, pack, prepare final products active seabuckthorn polypeptide.The method is a kind of with low cost, preparation method of product yield is high, polypeptide active is high active seabuckthorn polypeptide.
Description
Technical field
The present invention relates to technical field of bioengineering, the preparation method of a kind of active seabuckthorn polypeptide.
Background technology
Fructus Hippophae is a kind of fallen leaves property shrub, and drought-enduring, anti-blown sand can be survived on salt-soda soil, planted in a large number in NORTHWEST CHINA portion
Planting Fructus Hippophae, for water and soil conservation and desert afforestation, plant resources enriches.Sea buckthorn fruit nutritional labeling and pharmaceutical ingredient content are high,
Its medicines and health protection, clinical medicinal efficacy make it have high Development volue.In prior art, can be made into many for raw material with Fructus Hippophae
Kind of beverage/food and drinks, it is also possible to extracting Oleum Hippophae, Fructus Hippophae slag can also extract procyanidin and flavone, to Fructus Hippophae albumen and
The research of polypeptide is less.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that one is with low cost, product yield is high, polypeptide is alive
The preparation method of the active seabuckthorn polypeptide that property is high.
The technical scheme is that and be achieved in that: the preparation method of a kind of active seabuckthorn polypeptide, it includes following several
Individual step:
A sea buckthorn fruit is cleaned up by (), pulverize with beater or pulverizer, prepares and includes Fructus Hippophae sarcocarp and seed
Real Fructus Hippophae serosity；
B fresh Fructus Chaenomelis is cleaned up by (), peel, remove seed, squeeze the juice, and prepares Sucus Chaenomelis；
C () Fructus Hippophae serosity is mixed by the part by weight of 15～25: 1 with Sucus Chaenomelis, be heated to 50～60 DEG C, is incubated enzymolysis
3～5 hours, prepare Fructus Hippophae enzymolysis mixed liquor；
D Fructus Hippophae enzymolysis mixed liquor is heated to 80～100 DEG C by (), be incubated 10～40 minutes, make enzyme inactivate, and prepares enzyme and loses
The Fructus Hippophae enzymolysis mixed liquor lived；
E () adds the ethanol of 95 ° in the Fructus Hippophae enzymolysis mixed liquor that enzyme inactivates, make the alcohol content in liquid reach 60
～80 °, stand and activate 3～5 hours, then distill the ethanol in liquid, prepare the Fructus Hippophae enzymolysis mixed liquor activated；
F the Fructus Hippophae enzymolysis mixed liquor of activation is filtered by (), filter solid matter, then by many for the active seabuckthorn that obtains
Peptide solution carries out fill, sterilizing, packaging, prepares final products active seabuckthorn polypeptide.
Further, in step (a), also include grinding Fructus Hippophae sarcocarp and the seed of pulverizing further, use colloid barreling
Being milled to fineness is 130～150 mesh, prepares the Fructus Hippophae serosity including Fructus Hippophae sarcocarp and seed.
Present invention advantage compared to the prior art is:
1, the preparation method that the present invention provides is carried out at 50～60 DEG C of temperature and normal pressure；Avoid high temperature in traditional handicraft
Extract the destruction of active substance in sea buckthorn fruit, it is ensured that the biological activity of functional component in sea buckthorn fruit, significantly contract simultaneously
Short extraction time, improve production efficiency.
2, the raw material selected is sea buckthorn fruit and Fructus Chaenomelis, abundant raw material, especially by same to sarcocarp and the seed of sea buckthorn fruit
In time, pulverize as raw material, and production technology is simple, and nutritional labeling is comprehensive.
3, selecting Sucus Chaenomelis as enzyme source, taking full advantage of papain has vegetable protein, polypeptide, ester, amide etc.
Stronger enzymolysis ability, the most also has the ability of synthesis, can be protein hydrolysate resynthesis protein substance, Ke Yiti
The nutritive value of high sea buckthorn fruit.
4, the inactivation Fructus Hippophae enzymolysis mixed liquor obtained through enzymolysis and inactivation treatment, have passed through again ethanol activation and processes,
Have activated the activity of peptide, improve nutritional health function.
5, Technology is simple, it is not necessary to the operations such as NF membrane nanofiltration and spray drying, easily realizes Automated condtrol.
7, present invention process technology environmental protection, has thoroughly abandoned conventional organic solvent method of purification, to people in operating process
Member and surrounding do not produce harm.
Detailed description of the invention
Below in conjunction with embodiment, the method for the present invention is further illustrated.
Embodiment 1: the preparation method of a kind of active seabuckthorn polypeptide, it includes following step:
A sea buckthorn fruit is cleaned up by (), pulverize with beater or pulverizer, is ground to carefully with colloid mill the most again
Degree is 150 mesh, prepares the Fructus Hippophae serosity including Fructus Hippophae sarcocarp and seed；
B fresh Fructus Chaenomelis is cleaned up by (), peel, remove seed, squeeze the juice, and prepares Sucus Chaenomelis；
C () Fructus Hippophae serosity is mixed by the part by weight of 15: 1 with Sucus Chaenomelis, be heated to 60 DEG C, insulation enzymolysis 5 hours, system
Obtain Fructus Hippophae enzymolysis mixed liquor；
D Fructus Hippophae enzymolysis mixed liquor is heated to 80 DEG C by (), be incubated 30 minutes, make enzyme inactivate, and prepares the Fructus Hippophae of enzyme inactivation
Really enzymolysis mixed liquor；
E () adds the ethanol of 95 ° in the Fructus Hippophae enzymolysis mixed liquor that enzyme inactivates, make the alcohol content in liquid reach
80 °, stand and activate 3 hours, then distill the ethanol in liquid, prepare the Fructus Hippophae enzymolysis mixed liquor activated；
F the Fructus Hippophae enzymolysis mixed liquor of activation is filtered by (), filter solid matter, then by many for the active seabuckthorn that obtains
Peptide solution carries out fill, sterilizing, packaging, prepares final products active seabuckthorn polypeptide.
Embodiment 2: the preparation method of a kind of active seabuckthorn polypeptide, it includes following step:
A sea buckthorn fruit is cleaned up by (), pulverize with beater or pulverizer, is ground to carefully with colloid mill the most again
Degree is 140 mesh, prepares the Fructus Hippophae serosity including Fructus Hippophae sarcocarp and seed；
B fresh Fructus Chaenomelis is cleaned up by (), peel, remove seed, squeeze the juice, and prepares Sucus Chaenomelis；
C () Fructus Hippophae serosity is mixed by the part by weight of 20: 1 with Sucus Chaenomelis, be heated to 55 DEG C, insulation enzymolysis 4 hours, system
Obtain Fructus Hippophae enzymolysis mixed liquor；
D Fructus Hippophae enzymolysis mixed liquor is heated to 90 DEG C by (), be incubated 25 minutes, make enzyme inactivate, and prepares the Fructus Hippophae of enzyme inactivation
Really enzymolysis mixed liquor；
E () adds the ethanol of 95 ° in the Fructus Hippophae enzymolysis mixed liquor that enzyme inactivates, make the alcohol content in liquid reach
70 °, stand and activate 4 hours, then distill the ethanol in liquid, prepare the Fructus Hippophae enzymolysis mixed liquor activated；
F the Fructus Hippophae enzymolysis mixed liquor of activation is filtered by (), filter solid matter, then by many for the active seabuckthorn that obtains
Peptide solution carries out fill, sterilizing, packaging, prepares final products active seabuckthorn polypeptide.
Embodiment 3: the preparation method of a kind of active seabuckthorn polypeptide, it includes following step:
A sea buckthorn fruit is cleaned up by (), pulverize with beater or pulverizer, is ground to carefully with colloid mill the most again
Degree is 130 mesh, prepares the Fructus Hippophae serosity including Fructus Hippophae sarcocarp and seed；
B fresh Fructus Chaenomelis is cleaned up by (), peel, remove seed, squeeze the juice, and prepares Sucus Chaenomelis；
C () Fructus Hippophae serosity is mixed by the part by weight of 25: 1 with Sucus Chaenomelis, be heated to 50 DEG C, insulation enzymolysis 3 hours, system
Obtain Fructus Hippophae enzymolysis mixed liquor；
D Fructus Hippophae enzymolysis mixed liquor is heated to 95 DEG C by (), be incubated 20 minutes, make enzyme inactivate, and prepares the Fructus Hippophae of enzyme inactivation
Really enzymolysis mixed liquor；
E () adds the ethanol of 95 ° in the Fructus Hippophae enzymolysis mixed liquor that enzyme inactivates, make the alcohol content in liquid reach
60 °, stand and activate 5 hours, then distill the ethanol in liquid, prepare the Fructus Hippophae enzymolysis mixed liquor activated；
F the Fructus Hippophae enzymolysis mixed liquor of activation is filtered by (), filter solid matter, then by many for the active seabuckthorn that obtains
Peptide solution carries out fill, sterilizing, packaging, prepares final products active seabuckthorn polypeptide.
Claims (2)
1. a preparation method for active seabuckthorn polypeptide, it includes following step:
A sea buckthorn fruit is cleaned up by (), pulverize with beater or pulverizer, prepares and includes Fructus Hippophae sarcocarp and seed
Fructus Hippophae serosity；
B fresh Fructus Chaenomelis is cleaned up by (), peel, remove seed, squeeze the juice, and prepares Sucus Chaenomelis；
C () Fructus Hippophae serosity is mixed by the part by weight of 15～25: 1 with Sucus Chaenomelis, be heated to 50～60 DEG C, is incubated enzymolysis 3～5
Hour, prepare Fructus Hippophae enzymolysis mixed liquor；
D Fructus Hippophae enzymolysis mixed liquor is heated to 80～100 DEG C by (), be incubated 10～40 minutes, make enzyme inactivate, and prepares enzyme inactivation
Fructus Hippophae enzymolysis mixed liquor；
E () adds the ethanol of 95 ° in the Fructus Hippophae enzymolysis mixed liquor that enzyme inactivates, make the alcohol content in liquid reach 60～
80 °, stand and activate 3～5 hours, then distill the ethanol in liquid, prepare the Fructus Hippophae enzymolysis mixed liquor activated；
F the Fructus Hippophae enzymolysis mixed liquor of activation is filtered by (), filter solid matter, then by molten for the active seabuckthorn polypeptide that obtains
Liquid carries out fill, sterilizing, packaging, prepares final products active seabuckthorn polypeptide.
The preparation method of active seabuckthorn polypeptide the most according to claim 1, it is characterised in that: in step (a), also include
Fructus Hippophae sarcocarp and the seed of pulverizing being ground further, being ground to fineness with colloid mill is 130～150 mesh, prepares and includes sand
The Fructus Hippophae serosity of spine sarcocarp and seed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610379068.4A CN106072095A (en) | 2016-05-30 | 2016-05-30 | The preparation method of active seabuckthorn polypeptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610379068.4A CN106072095A (en) | 2016-05-30 | 2016-05-30 | The preparation method of active seabuckthorn polypeptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106072095A true CN106072095A (en) | 2016-11-09 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610379068.4A Pending CN106072095A (en) | 2016-05-30 | 2016-05-30 | The preparation method of active seabuckthorn polypeptide |
Country Status (1)
| Country | Link |
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| CN (1) | CN106072095A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475336A (en) * | 2017-08-09 | 2017-12-15 | 郝之奎 | A kind of preparation method of Papain peptide |
| CN114196721A (en) * | 2021-12-23 | 2022-03-18 | 湖北瑞邦生物科技有限公司 | Sea-buckthorn bioactive peptide and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104099393A (en) * | 2014-07-23 | 2014-10-15 | 山西大学 | Sea-buckthorn seed polypeptide with inhibiting effect on intestinal pathogens as well as preparation method and application of sea-buckthorn seed polypeptide |
| CN105063139A (en) * | 2015-07-17 | 2015-11-18 | 山西大学 | Preparation method of sea-buckthorn seed polypeptide used for sobering up from drunkenness |
-
2016
- 2016-05-30 CN CN201610379068.4A patent/CN106072095A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104099393A (en) * | 2014-07-23 | 2014-10-15 | 山西大学 | Sea-buckthorn seed polypeptide with inhibiting effect on intestinal pathogens as well as preparation method and application of sea-buckthorn seed polypeptide |
| CN105063139A (en) * | 2015-07-17 | 2015-11-18 | 山西大学 | Preparation method of sea-buckthorn seed polypeptide used for sobering up from drunkenness |
Non-Patent Citations (1)
| Title |
|---|
| 艾遥琴等: "木瓜大豆多肽饮料的制备", 《食品工业》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475336A (en) * | 2017-08-09 | 2017-12-15 | 郝之奎 | A kind of preparation method of Papain peptide |
| CN114196721A (en) * | 2021-12-23 | 2022-03-18 | 湖北瑞邦生物科技有限公司 | Sea-buckthorn bioactive peptide and preparation method thereof |
| CN114196721B (en) * | 2021-12-23 | 2023-11-21 | 湖北瑞邦生物科技有限公司 | Sea buckthorn bioactive peptide and preparation method thereof |
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Application publication date: 20161109 |
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