CN106070090B - A kind of raw survey device of brown paddy plant hopper - Google Patents

A kind of raw survey device of brown paddy plant hopper Download PDF

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Publication number
CN106070090B
CN106070090B CN201610677551.0A CN201610677551A CN106070090B CN 106070090 B CN106070090 B CN 106070090B CN 201610677551 A CN201610677551 A CN 201610677551A CN 106070090 B CN106070090 B CN 106070090B
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cuboid
square
cylindrical pipe
raw
partition
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CN106070090A (en
Inventor
邵恩斯
林莉
陈晨
王志强
陈汉泽
张娇
关雄
黄志鹏
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Fujian Lu'an biological pesticide Co., Ltd
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Fujian Agriculture and Forestry University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

A kind of the raw of brown paddy plant hopper of the present invention surveys device, a kind of raw survey device that can carry out the raw survey experiment of multiple groups test worm simultaneously is provided, including transparent, inner hollow square or cuboid, each side of the square or cuboid is equipped with the first round hole, the top surface of the square or cuboid is equipped with the second round hole, and the square or cuboid are equipped with gap;It further include cylindrical pipe, partition and rubber stopper, the cylindrical pipe is transparent cylindrical pipe, the both ends of the cylindrical pipe are equipped with aperture, the area of the aperture is equal with cylindrical pipe floor space, the cylindrical pipe is inserted into first round hole, and the partition is inserted into the gap, and the partition is used to completely cut off inner cavity and the cylindrical pipe of square or cuboid, the rubber stopper is inserted into second round hole, and the bottom of the rubber stopper hangs with wet cotton balls or agar block.

Description

A kind of raw survey device of brown paddy plant hopper
Technical field
The present invention relates to experiment consumptive materials, and in particular to a kind of raw survey device of brown paddy plant hopper.
Background technique
Sucking pest uses needle-shaped mouthpart feeding plant tissue juice, can not feeding food, needle in the prior art Its sod cultivation is established to the feeding characteristic of sucking pest, traditional raw survey device uses the glass tube of both ends open, Feed, other end nylon gauze and rubber band sealing are placed with film feeding method in one end, keep inner air tube circulation.But the life is surveyed The shortcoming of device is apparent: (1) using rubber band nylon gauze is fixed on glass tube one end, easily generation gap, Test worm is stranded after entering gap and cannot escape i.e. death, influences the raw accuracy for surveying result;(2) when replacing feed, process is solid Determine, cannot be interrupted, test worm is easily escaped, and raw survey as a result, operation exception is cumbersome is influenced.(3) air humidity in test tube is not can guarantee.It is brown Plant hopper is a kind of common sucking pest, and demand of the brown paddy plant hopper as rice grub to humidity is high, and suitable growth is wet Degree is 80% or more.If it cannot be guaranteed that the humidity that subenvironment maintains polypide suitable in glass tube, easily causes test worm dead, influences It is raw to survey result.
As an improvement, disclosing the raw of sucking pest application No. is 201510276569.5 Chinese invention patent surveys dress It sets and its sod cultivation, including a transparent vial body and a removable bottle cap, the bottom of bottle is added with Ago-Gel, clear vial Air hole is offered in the bottle wall of body, is pasted on the inside of bottle wall on the air hole and is covered with medical adhesive tape of breathing freely, it is described Bottle cap offers feeding mouth, the bottle cap film for having the Parafilm of stretching to be formed on the feeding mouth from bottle covering mouth, bottle cap film Upper to be added with liquid food, the upper surface of liquid food has food protective film.Although the raw of the invention surveys device and sod cultivation It prevents test worm to escape to a certain extent, maintains the raw humidity surveyed in device, but the life surveys device and can only once carry out one The raw of group test worm surveys experiment, and due to every group of bottle or pipe, leading to raw survey condition between each group, there are certain errors, in turn Influence the raw accuracy for surveying experimental result.
Therefore, it is necessary to invent a kind of raw survey device that can carry out the raw survey experiment of multiple groups test worm simultaneously.
Summary of the invention
The technical problems to be solved by the present invention are: providing a kind of raw survey dress that can carry out the raw survey experiment of multiple groups test worm simultaneously It sets.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention are as follows:
A kind of the raw of brown paddy plant hopper surveys device, including transparent, inner hollow square or cuboid, the square or Each side of cuboid is equipped with the first round hole, and the top surface of the square or cuboid is equipped with the second round hole, it is described just Cube or cuboid are equipped with gap;It further include cylindrical pipe, partition and rubber stopper, the cylindrical pipe is transparent cylindrical body Pipe, the both ends of the cylindrical pipe are equipped with aperture, and the area of the aperture is equal with cylindrical pipe floor space, the cylindrical body Pipe is inserted into first round hole, and the partition is inserted into the gap, and the partition is used to completely cut off the interior of square or cuboid Chamber and cylindrical pipe, the rubber stopper are inserted into second round hole, and the bottom of the rubber stopper hangs with wet cotton balls or agar block.
Further, one end of the cylindrical tube is covered with the first sealed membrane, is added with liquid on first sealed membrane Body food, the other end of liquid food are covered with the second sealed membrane.
Further, first sealed membrane and the second sealed membrane are parafilm plastic seal membrana oralis.
Further, the square or cuboid are acrylic square or cuboid, and the cylindrical pipe is plastics Cylindrical pipe or acrylic cylindrical pipe.
Further, the partition includes gauze, and four sides of the gauze are equipped with frame, and the frame is hardboard frame Or acrylic frame.
Further, the pore size of the gauze is 0.8-1.2mm.
Further, the diameter of the cylindrical pipe is 25-30mm, and the height of the cylindrical pipe is 25-35mm, described Gap is 8-12mm at a distance from the most near side (ns) of square or cuboid.
The beneficial effects of the present invention are: pass through the raw test tube of four side carry cylindrical bodies using square or cuboid Raw survey of duplicature feeding for carrying out brown paddy plant hopper is tested, and the raw efficiency surveyed of film feeding method is improved;By utilizing square or rectangular Wet cotton balls or agar block are hung at the top of body, provides consistent humidity for the life test tube on 4 sides, improve raw survey experimental data Stability and experimental result accuracy;By detachably giving birth to test tube, the filling of raw test worm is simplified, death rate meter is improved Number efficiency, and it is able to achieve quick-replaceable feeding solution.
Detailed description of the invention
Fig. 1 is the structural schematic diagram for surveying device in all one's life of the embodiment of the present invention;
Fig. 2 is the structural schematic diagram for surveying rubber stopper in device in all one's life of the embodiment of the present invention;
Fig. 3 is the structural schematic diagram for surveying partition in device in all one's life of the embodiment of the present invention.
Label declaration:
1- square or cuboid;The second round hole of 2-;The gap 3-;4- cylindrical pipe;5- partition;51- gauze;The side 52- Frame;6- rubber stopper;The wet cotton balls of 7- or agar block.
Specific embodiment
To explain the technical content, the achieved purpose and the effect of the present invention in detail, below in conjunction with embodiment and cooperate attached Figure is explained.
Please refer to Fig. 1 to Fig. 3, a kind of the raw of brown paddy plant hopper surveys device, including transparent, inner hollow square or rectangular Each side of body 1, the square or cuboid 1 is equipped with the first round hole, and the top surface of the square or cuboid 1 is equipped with Second round hole 2, the square or cuboid 1 are equipped with gap 3;It further include cylindrical pipe 4, partition 5 and rubber stopper 6, institute Stating cylindrical pipe 4 is transparent cylindrical pipe 4, and the both ends of the cylindrical pipe 4 are equipped with aperture, the area and circle of the aperture 4 floor space of cylinder pipe is equal, and the cylindrical pipe 4 is inserted into first round hole, and the partition 5 is inserted into the gap 3, described Partition 5 is used to completely cut off inner cavity and the cylindrical pipe 4 of square or cuboid 1, and the rubber stopper 6 is inserted into second round hole 2, The bottom of the rubber stopper 6 hangs with wet cotton balls or agar block 7.
Further, gap 3 everywhere are respectively arranged in the corresponding position in the bottom surface and top surface of the square or cuboid 1; Optionally, the gap 3 at the inside setting two on the side of the bottom surface and top surface of the square or cuboid 1 being parallel to each other, Gap 3 at the two sides corresponding position setting two of square or cuboid 1 being parallel to each other.
Further, the square or cuboid 1 or other cubes, such as triangular prism, pentagonal prism, hexagonal prisms Equal polygon prisms, rotary table and other cubes.
As can be seen from the above description, the beneficial effects of the present invention are: pass through four sides using square or cuboid The raw test tube of carry cylindrical body carries out the raw survey experiment of duplicature feeding of brown paddy plant hopper, improves the raw efficiency surveyed of film feeding method;Pass through Using wet cotton balls or agar block is hung at the top of square or cuboid, consistent humidity is provided for the life test tube on 4 sides, is mentioned The high accuracy of the raw stability for surveying experimental data and experimental result;By detachably giving birth to test tube, raw test worm is simplified Filling improves death rate counting efficiency, and is able to achieve quick-replaceable feeding solution.
Further, one end of the cylindrical tube 4 is covered with the first sealed membrane, is added with liquid on first sealed membrane Body food, the other end of liquid food are covered with the second sealed membrane.
Further, first sealed membrane and the second sealed membrane are parafilm plastic seal membrana oralis.
Further, the square or cuboid 1 are acrylic square or cuboid, and the cylindrical pipe 4 is modeling Expect cylindrical pipe or acrylic cylindrical pipe.
Further, the partition 5 includes gauze 51, and four sides of the gauze 51 are equipped with frame, and the frame is cardboard Edges of boards frame or acrylic frame.
Further, the pore size of the gauze is 0.8-1.2mm.
Further, the diameter of the cylindrical pipe 4 is 25-30mm, and the height of the cylindrical pipe 4 is 25-35mm, institute It is 8-12mm that gap 3, which is stated, at a distance from the most near side (ns) of square or cuboid 1.
The raw application method for surveying device of the present invention are as follows: when carrying out brown paddy plant hopper life survey, 4 cylindrical pipes are first taken, in pipe One end covering stretch after parafilm plastic seal membrana oralis, be added dropwise on film and need the solution that feeds, then with after one layer of stretching Parafilm sealed membrane cover solution, form the structure of duplicature.Then, by 4 cylindrical pipes be inserted into respectively square or In first round hole of 4 sides of cuboid, and the partition is inserted at the gap of respective side, by cylindrical pipe and just Cube or cuboid intracavity space separate.
When loading first group of test worm, the brown paddy plant hopper nymph 10-20 of 5min will be handled at 4 DEG C only by square or cuboid Top opening (the second round hole) be put into square or cuboid, open side partition, pat square or cuboid, will Test worm closes partition after being put into a side pipe.Second group of test worm is put into square again, opens second partition.And so on, 4 groups of test worms are put into the cylindrical pipe of 4 sides respectively and close partition.The bottom of rubber stopper is hung over after the cotton balls is got wet Or agar block is hung over into rubber stopper bottom, then rubber stopper is clogged to the top opening of square or cuboid, moist cotton balls is hanged The centre of square or cuboid is hung on to provide humidity into 4 cylindrical pipes.Square or cuboid are placed in constant temperature again Raw survey is carried out in incubator to test.
To replacement feeding solution during raw survey, then moist cotton balls is first taken out, then stopper rubber stopper, open and need more Test worm is slowly trembled the inner cavity such as square or cuboid, partition is turned back to by the partition for changing solution side, then can place test worm The duplicature and feeding solution of test worm nozzle are smoothly replaced under the premise of escape.After replacement, then partition is opened, test worm is trembled into circle In cylinder pipe, partition, which turns back to, continues raw survey.
The raw calculating for surveying the death rate can first open rubber stopper, remove cotton balls, stopper rubber stopper, and opening needs counting experiments group Test worm is poured into square or cuboid, turns back to partition by partition.Rubber stopper is then opened, test worm is observed by top open part Death condition, and available fine, soft fur pen protrudes into inside square and touches test worm to determine that its is dead.
Embodiment one
Please refer to Fig. 1 to Fig. 3, a kind of the raw of brown paddy plant hopper surveys device, including transparent, inner hollow square or rectangular Each side of body 1, the square or cuboid 1 is equipped with the first round hole, and the top surface of the square or cuboid 1 is equipped with Second round hole 2, the square or cuboid 1 are equipped with gap 3;It further include cylindrical pipe 4, partition 5 and rubber stopper 6, institute Stating cylindrical pipe 4 is transparent cylindrical pipe 4, and the both ends of the cylindrical pipe 4 are equipped with aperture, the area and circle of the aperture 4 floor space of cylinder pipe is equal, and the cylindrical pipe 4 is inserted into first round hole, and the partition 5 is inserted into the gap 3, described Partition 5 is used to completely cut off inner cavity and the cylindrical pipe 4 of square or cuboid 1, and the rubber stopper 6 is inserted into second round hole 2, The bottom of the rubber stopper 6 hangs with wet cotton balls or agar block 7.
One end of the cylindrical tube 4 is covered with the first sealed membrane, is added with liquid food, liquid on first sealed membrane The other end of body food is covered with the second sealed membrane;First sealed membrane and the second sealed membrane are parafilm plastic closures Film;The square or cuboid 1 are acrylic square or cuboid, and the cylindrical pipe 4 is plastic cylinder pipe or Asia Gram force cylindrical pipe;The partition 5 includes gauze 51, and four sides of the gauze 51 are equipped with frame, and the frame is cardboard edges of boards Frame or acrylic frame;The pore size of the gauze is 0.8-1.2mm, preferably 1.0mm;The diameter of the cylindrical pipe 4 is 25-30mm, preferably 28mm, the height of the cylindrical pipe 4 are 25-35mm, preferably 30mm, the gap 3 and square or length The distance of the most near side (ns) of cube 1 is 8-12mm, preferably 10mm.
In conclusion the raw of brown paddy plant hopper provided by the invention surveys device, pass through four sides using square or cuboid Carry cylindrical body raw test tube in face carries out the raw survey experiment of duplicature feeding of brown paddy plant hopper, improves the raw efficiency surveyed of film feeding method;It is logical It crosses using wet cotton balls or agar block is hung at the top of square or cuboid, provides consistent humidity for the life test tube on 4 sides, Improve the accuracy of the raw stability for surveying experimental data and experimental result;By detachably giving birth to test tube, raw test worm is simplified Filling, improve death rate counting efficiency, and be able to achieve quick-replaceable feeding solution.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair Equivalents made by bright specification and accompanying drawing content are applied directly or indirectly in relevant technical field, similarly include In scope of patent protection of the invention.

Claims (6)

1. a kind of the raw of brown paddy plant hopper surveys device, which is characterized in that including transparent, inner hollow square or cuboid, institute The each side for stating square or cuboid is equipped with the first round hole, and it is round that the top surface of the square or cuboid is equipped with second Hole, the square or cuboid are equipped with gap;It further include cylindrical pipe, partition and rubber stopper, the cylindrical pipe is Bright cylindrical pipe, the both ends of the cylindrical pipe are equipped with aperture, and the area of the aperture is equal with cylindrical pipe floor space, The cylindrical pipe is inserted into first round hole, and the partition is inserted into the gap, the partition for completely cut off square or The inner cavity of cuboid and cylindrical pipe, the rubber stopper are inserted into second round hole, and the bottom of the rubber stopper hangs with wet cotton Ball or agar block.
2. a kind of the raw of brown paddy plant hopper according to claim 1 surveys device, which is characterized in that cover one end of the cylindrical tube It is stamped the first sealed membrane, is added with liquid food on first sealed membrane, the other end of liquid food is covered with the second sealing Film.
3. a kind of the raw of brown paddy plant hopper according to claim 2 surveys device, which is characterized in that first sealed membrane and second Sealed membrane is parafilm plastic seal membrana oralis.
4. a kind of the raw of brown paddy plant hopper according to claim 1 surveys device, which is characterized in that the square or cuboid are Acrylic square or cuboid, the cylindrical pipe are plastic cylinder pipe.
5. a kind of the raw of brown paddy plant hopper according to claim 1 surveys device, which is characterized in that the partition includes gauze, institute Four sides for stating gauze are equipped with frame, and the frame is hardboard frame or acrylic frame.
6. a kind of the raw of brown paddy plant hopper according to claim 5 surveys device, which is characterized in that the pore size of the gauze is 0.8-1.2mm。
CN201610677551.0A 2016-08-17 2016-08-17 A kind of raw survey device of brown paddy plant hopper Active CN106070090B (en)

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Publication number Priority date Publication date Assignee Title
CN111066735A (en) * 2019-12-24 2020-04-28 深圳市铁汉生态环境股份有限公司 Feeding device for measuring feeding amount of insects

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CN101707991B (en) * 2009-12-25 2013-04-10 浙江省农业科学院 Artificial spawning and breeding device suitable for piercing-sucking small insects
CN202112170U (en) * 2011-06-03 2012-01-18 华南农业大学 Artificial feeding device
CN203952165U (en) * 2014-07-21 2014-11-26 傅淑 A kind of new device of raising piercing sucking insect
CN204466637U (en) * 2015-02-05 2015-07-15 湖南省植物保护研究所 Piercing sucking insect rearing-box
CN204682225U (en) * 2015-05-26 2015-10-07 中国农业科学院植物保护研究所 The raw of sucking pest surveys device
CN104839098A (en) * 2015-05-26 2015-08-19 中国农业科学院植物保护研究所 Device and method for bioassay of piercing-sucking pests
CN204930035U (en) * 2015-09-08 2016-01-06 中国农业科学院棉花研究所 A kind of piercing-sucking mouthparts pests raising device

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Effective date of registration: 20200612

Address after: No.17, Nanpu eco industrial park, Pucheng County, Nanping City, Fujian Province, 353499

Patentee after: Fujian Lu'an biological pesticide Co., Ltd

Address before: Cangshan District of Fuzhou City, Fujian province 350002 to build a new town, Jinshan District

Patentee before: FUJIAN AGRICULTURE AND FORESTRY University

PE01 Entry into force of the registration of the contract for pledge of patent right
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of invention: A bioassay device for Brown Planthopper

Effective date of registration: 20200825

Granted publication date: 20190115

Pledgee: Industrial Bank Nanping branch of Limited by Share Ltd Pucheng

Pledgor: Fujian Lu'an biological pesticide Co., Ltd

Registration number: Y2020980005310