CN104839098A - Device and method for bioassay of piercing-sucking pests - Google Patents
Device and method for bioassay of piercing-sucking pests Download PDFInfo
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- CN104839098A CN104839098A CN201510276569.5A CN201510276569A CN104839098A CN 104839098 A CN104839098 A CN 104839098A CN 201510276569 A CN201510276569 A CN 201510276569A CN 104839098 A CN104839098 A CN 104839098A
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- bottle
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Animal Behavior & Ethology (AREA)
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- Biodiversity & Conservation Biology (AREA)
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Abstract
The invention relates to a device and a method for bioassay of piercing-sucking pests. The device comprises a transparent bottle body and a detachable bottle cover, agarose gel is added at a bottle bottom, a ventilating hole is formed in a bottle wall of the transparent bottle body, a ventilating medical transparent adhesive tape covers the ventilating holes in a pasted manner from the inner side of the bottle wall, the bottle cover is provided with a food fetching port, a bottle cover film formed by sealing by a stretched paraffin sealing film from the outside of the bottle body is arranged on the food fetching port, liquid food is added on the bottle cover film, and a food protection film is adhered on the liquid food. By the device and the method, escape of test pests can be avoided while sufficient air can be provided for growing of the test pests; preparation of feed for bioassay can be completed by using the bottle cover only; agarose gel can maintain humidity, suitable for laodelphax striatellus, in the bottle body, bioassay experiments of the piercing-sucking pests can be completed in incubators which are good in temperature control effect, and mortality rate, floating above or below 20%, of a bioassay comparison treatment group in the past is lowered to be below 10%.
Description
Technical field
The present invention relates to biological control research field, particularly relate to the raw of sucking pest and survey device and sod cultivation thereof.
Background technology
Sucking pest adopts needle-like mouthpart feeding plant to organize juice, food cannot be taken food, the characteristic that takes food for sucking pest in prior art establishes its sod cultivation, traditional raw survey system adopts the glass tube of both ends open, at one end place feed with film feeding method, other end nylon gauze and rubber band sealing, holding tube inner air flow.But the method is existing defects still, need improvement badly.The method has three obvious shortcomings, 1.) utilizes rubber band that nylon gauze is fixed on glass tube one end, very easily produces gap, stranded and can not escape namely dead after examination worm enters gap, the raw accuracy surveying result of impact.2. when) changing feed, flow process is fixed, can not be interrupted, and examination worm easily runs away, and impact is raw surveys result, and operation exception is loaded down with trivial details.3.) in vitro air humidity cannot be ensured.Rice fulgorid is high as the demand of rice grub to humidity, and its suitable growth humidity is more than 80%.The northern area of China, air oxygen detrition, even if use constant temperature and humidity incubator, also can not ensure that in glass tube, subenvironment maintains the suitable humidity of polypide, easily cause examination worm dead, impact is raw surveys result.Owing to there are the problems referred to above, blank examination worm lethality fluctuates 20%, and when blank lethality is greater than 20%, then raw data invalid of surveying, result in the waste of examination worm and test proteins.
Summary of the invention
For the difference in above-mentioned field, the invention provides the raw of a kind of sucking pest and survey device, blank examination worm lethality is low, can produce more accurately result reliably, for high-throughout insecticidal activity bacterial strain screening, and protein active checking provides the reliable guarantee of experimental technique aspect.
The sod cultivation adopting this life to survey device is also provided simultaneously.
The raw of sucking pest surveys device; comprise a transparent vial body and a removable bottle cap; it is characterized in that: at the bottom of described bottle, be added with Ago-Gel; the bottle wall of transparent vial body offers air-vent; described air-vent is pasted inside bottle wall and is coated with ventilative medical adhesive tape, described bottle cap offers and takes food mouth, described in take food on mouth and have the Parafilm of stretching from the bottle cap film of bottle covering interruption-forming; bottle cap film is added with liquid food, with food diaphragm above liquid food.
Described food diaphragm is the Parafilm stretched, and is fixed on bottle cap.
The volume 50mL of described transparent vial body, bottle height 69mm, diameter 42mm, described in take food mouth be diameter is the hole of 30mm, described air-vent is diameter is 6mm.
4mL 2% Ago-Gel is added with at the bottom of described bottle.
Described transparent vial body is transparent PET plastic bottle, and described bottle cap film is double-deck Parafilm.
Described bottle cap and bottle are for being threaded.
A sod cultivation for sucking pest, comprises the steps:
(1) prepare the raw of above-mentioned sucking pest and survey device;
(2) examination worm is put in bottle; Cover body with black cloth, by bottleneck towards light source, change feed every three days;
(3) raw survey continues 3-6d.
The preparation method of described step (1) is as follows: get transparent plastic bottle, bottle cap diameter 30mm hole making drill punching, the 6mm drill bit perforate of body side; In bottle, paste medical air-permeable adhesive tape seal bottle hole, the lid of perforate is covered after being stretched by double-deck Parafilm, adding liquid food, be overlying on liquid food with the Parafilm stretched again and seal, be made into feed capsule, add 4mL 2% Ago-Gel at the bottom of bottle with sucking pest described in moisturizing for small brown rice planthopper.
Described replacing feed is changed together by the bottle cap with feed capsule.
Examination worm 20 is put into, each process repetition 3 times in described bottle.
Present invention employs plastic bottle, and the hole of 6mm diameter is opened in its body side, and in bottle, paste ventilative medical adhesive tape seal perforate, can prevent from trying worm and escape and can provide enough air for the growth trying worm again, solve the unexpected death of examination worm to raw impact of surveying result.Secondly, bottle cap is separable with body, can to complete the preparation of raw survey feed with bottle cap.The feed that need replace and bottle cap together take off, and more renew.Whole process can be carried out step by step, is beneficial to operation, reduces examination worm and escapes, greatly facilitate raw the carrying out surveying experiment.Finally, the good incubator of temperature control at the bottom of bottle, adds the Ago-Gel of 2% concentration, humidity suitable to small brown rice planthopper in bottle can be maintained, as long as all can carry out the raw of sucking pest survey experiment, the lethality that this measure made survey control treatment group first previous existence to fluctuate 20%, is down to less than 10%.
Accompanying drawing explanation
The raw structural representation surveying device of Fig. 1 sucking pest of the present invention,
The raw cross-sectional schematic surveying bottle cap in device of Fig. 2 sucking pest of the present invention,
1-transparent vial body, 2-bottle cap, 3-air-vent, 4-ventilative medical adhesive tape, 5-take food mouth, 6-bottle cap film, 7-liquid food, 8-food diaphragm, 9-Ago-Gel.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail.
Embodiment 1
As Fig. 1, shown in 2, the raw of sucking pest surveys device, comprise the bottle cap 2 that a transparent vial body 1 and an employing are threaded, the volume 50mL of described transparent vial body 1, bottle height 69mm, diameter 42mm, 4mL 2% Ago-Gel 9 is added with at the bottom of described bottle, the bottle wall of transparent vial body 1 offers the air-vent 3 that diameter is 6mm, described air-vent 3 is pasted inside bottle wall and is coated with 3M and breathes freely medical adhesive tape 4, it is that 30mm takes food mouth 5 that described bottle cap 2 offers diameter, described taking food on mouth 5 has the double-deck Parafilm of stretching from the bottle cap film 6 of bottle covering interruption-forming, bottle cap film 6 is added with liquid food 7, with food diaphragm 8 above liquid food 7.Described food diaphragm 8 adopts the Parafilm stretched, and is fixed on bottle cap 2.Parafilm tundish is wrapped with liquid food, feed capsule.
Embodiment 2 sod cultivation
A. raw survey is used transparent PET plastic bottle (volume 50mL, bottle height 69mm, diameter 42mm) instead and is carried out;
B. white bottle cap, punches with diameter 30mm hole making drill; The 6mm drill bit perforate of body side, pastes 3M medical air-permeable adhesive tape in bottle
(
http:// solutions.3m.com.cn/wps/portal/3M/zh_CN/APACNexcare/Nexc are/Brand/tape/product2/), for the ventilation of examination worm;
C. 4mL 2% Ago-Gel is added at the bottom of bottle with moisturizing;
D. double-deck Parafilm (parafilm M, USA) is stretched and cover the lid of perforate after 4 times, add 200 μ L artificial feed;
E. be overlying on artificial feed with the Parafilm stretched again and seal, be made into feed capsule;
F. examination worm 20 is put into, each process repetition 3 times for every bottle;
G. cover body with black cloth, by bottleneck towards light source, change feed every three days.Raw survey continues 3-6d.
The foundation of the raw survey system of embodiment 3 and checking
On the basis being based upon original raw survey system of new raw survey system, improve through multiple spot.First, have employed plastic bottle, and open the hole of 6mm diameter in its body side, and in bottle, paste ventilative medical adhesive tape seal perforate, can prevent trying worm escape and can provide enough air for the growth trying worm again, solve the impact of examination worm unexpected death on raw survey result.Secondly, bottle cap is separable with body, can to complete the preparation of raw survey feed with bottle cap.The feed that need replace and bottle cap together take off, and more renew.Whole process can be carried out step by step, is beneficial to operation, reduces examination worm and escapes, greatly facilitate raw the carrying out surveying experiment.Finally, the good incubator of temperature control at the bottom of bottle, adds the Ago-Gel of 2% concentration, humidity suitable to small brown rice planthopper in bottle can be maintained, as long as all can carry out the raw of small brown rice planthopper survey experiment, the lethality that this measure made survey control treatment group first previous existence to fluctuate 20%, is down to less than 10%.
Claims (10)
1. the raw of sucking pest surveys device; comprise a transparent vial body and a removable bottle cap; it is characterized in that: at the bottom of described bottle, be added with Ago-Gel; the bottle wall of transparent vial body offers air-vent; described air-vent is pasted inside bottle wall and is coated with ventilative medical adhesive tape, described bottle cap offers and takes food mouth, described in take food on mouth and have the Parafilm of stretching from the bottle cap film of bottle covering interruption-forming; bottle cap film is added with liquid food, with food diaphragm above liquid food.
2. the raw of sucking pest according to claim 1 surveys device, and described food diaphragm is the Parafilm stretched, and is fixed on bottle cap.
3. the raw of sucking pest according to claim 1 surveys device, the volume 50mL of described transparent vial body, bottle height 69mm, diameter 42mm, described in take food mouth be diameter is the hole of 30mm, described air-vent is diameter is 6mm.
4. the raw of sucking pest according to claim 3 surveys device, is added with 4mL 2% Ago-Gel at the bottom of described bottle.
5. the raw of sucking pest according to claim 1 surveys device, and described transparent vial body is transparent PET plastic bottle, and described bottle cap film is double-deck Parafilm.
6. the raw of sucking pest according to claim 1 surveys device, and described bottle cap and bottle are for being threaded.
7. the sod cultivation of sucking pest, comprises the steps:
(1) prepare the raw of the arbitrary sucking pest of claim 1-6 and survey device;
(2) examination worm is put in bottle; Cover body with black cloth, by bottleneck towards light source, change feed every three days;
(3) raw survey continues 3-6d.
8. the sod cultivation of sucking pest according to claim 7, the preparation method of described step (1) is as follows: the transparent plastic bottle of try to please long-pending 50mL, bottle height 69mm, diameter 42mm, bottle cap diameter 30mm hole making drill punching, the 6mm drill bit perforate of body side; In bottle, paste medical air-permeable adhesive tape seal bottle hole, will double-deck Parafilm stretch after cover the lid of perforate, adding liquid food, then be overlying on liquid food with the Parafilm of stretching and seal, be made into feed capsule, add 4mL2% Ago-Gel at the bottom of bottle with moisturizing.
9. the sod cultivation of sucking pest according to claim 7, described replacing feed is changed together by the bottle cap with feed capsule.
10. the sod cultivation of sucking pest according to claim 7, the volume 50mL of described transparent vial body, bottle height 69mm, diameter 42mm, put into examination worm 20 in described bottle, each process repetition 3 times, described examination worm is small brown rice planthopper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510276569.5A CN104839098A (en) | 2015-05-26 | 2015-05-26 | Device and method for bioassay of piercing-sucking pests |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510276569.5A CN104839098A (en) | 2015-05-26 | 2015-05-26 | Device and method for bioassay of piercing-sucking pests |
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| CN104839098A true CN104839098A (en) | 2015-08-19 |
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| CN201510276569.5A Pending CN104839098A (en) | 2015-05-26 | 2015-05-26 | Device and method for bioassay of piercing-sucking pests |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106070090A (en) * | 2016-08-17 | 2016-11-09 | 福建农林大学 | The raw of a kind of brown paddy plant hopper surveys device |
| CN106719459A (en) * | 2017-01-11 | 2017-05-31 | 贵州大学 | A kind of piercing-sucking mouthparts pests liquid feed feed rack and feeding method |
| CN111557275A (en) * | 2019-02-14 | 2020-08-21 | 北京市农林科学院 | Do special simple and easy device of thrips test |
| CN114158525A (en) * | 2021-11-11 | 2022-03-11 | 西南大学 | Method for rapidly and accurately selecting panonychus citri in field |
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| CN101707991A (en) * | 2009-12-25 | 2010-05-19 | 浙江省农业科学院 | Artificial spawning and breeding device suitable for piercing-sucking small insects |
| CN202112170U (en) * | 2011-06-03 | 2012-01-18 | 华南农业大学 | Artificial feeding device |
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| CN102524188A (en) * | 2012-01-20 | 2012-07-04 | 福建省农业科学院植物保护研究所 | Insect breeding bottom and breeding method of gynaikothrips ficorum |
| CN203262057U (en) * | 2013-03-28 | 2013-11-06 | 天津市植物保护研究所 | Simple artificial rearing device for Chinese chive maggots |
| CN203860262U (en) * | 2014-05-14 | 2014-10-08 | 中国农业科学院烟草研究所 | Simple aphid feeding device |
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2015
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106070090A (en) * | 2016-08-17 | 2016-11-09 | 福建农林大学 | The raw of a kind of brown paddy plant hopper surveys device |
| CN106719459A (en) * | 2017-01-11 | 2017-05-31 | 贵州大学 | A kind of piercing-sucking mouthparts pests liquid feed feed rack and feeding method |
| CN111557275A (en) * | 2019-02-14 | 2020-08-21 | 北京市农林科学院 | Do special simple and easy device of thrips test |
| CN114158525A (en) * | 2021-11-11 | 2022-03-11 | 西南大学 | Method for rapidly and accurately selecting panonychus citri in field |
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Application publication date: 20150819 |