CN106045772A - Microorganism product capable of effectively reducing greenhouse gas emission of farmland soil and preparation method of microorganism product - Google Patents
Microorganism product capable of effectively reducing greenhouse gas emission of farmland soil and preparation method of microorganism product Download PDFInfo
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- CN106045772A CN106045772A CN201610372666.9A CN201610372666A CN106045772A CN 106045772 A CN106045772 A CN 106045772A CN 201610372666 A CN201610372666 A CN 201610372666A CN 106045772 A CN106045772 A CN 106045772A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a microorganism product capable of effectively reducing greenhouse gas emission of farmland soil and a preparation method of the microorganism product. The microorganism product is obtained by adding brevibacterium frigoritolerans and bacillus amyloliquefaciens in corn flour and vegetable garden soil mixture faeces, stirring evenly, and then standing for 4-6 days, and weight ratio of corn flour and vegetable garden soil in the corn and vegetable garden soil mixture faeces is 1:1. The microorganism product has the advantages of simplicity, convenience, low cost and easiness in operation, and a feasible operation method and a technology are provided for tackling of climate change and reduction of greenhouse gas emission in the agriculture. In an optional farmland, before seeding or transplanting of crops, 100 kilograms of the product is applied in every mu of the farmland while fertilization, emission of methane can be reduced by 35-56%, emission of nitrous oxide is reduced by 48-69%, and meanwhile, the yield of crops can be improved by 3-6%.
Description
Technical field
The present invention relates to greenhouse gases prevention and control environmental technology field, a kind of effectively minimizing agricultural land soil greenhouse gas
Microbial product of body discharge and preparation method thereof.
Background technology
Soil is the indispensable material base of plant growing, and release fats up, accumulates air, keeps moisture, storage energy
Deng, it is the valuable source of agricultural production.Soil is also the base camp of microorganism simultaneously, and substantial amounts of microorganism of surviving, this is slightly
Biological activity, promotes C on the earth, the biogeochemical cycle of the element such as H, O, N, P, S, remains element in air
Balance and ecological balance.
Agricultural soil is cultivated due to artificial, uses chemical fertilizer in a large number, has substantial amounts of plant residue to be accumulated in soil every year,
Through the decomposition and inversion of Soil Microorganism, become CO2、CH4、N2O isothermal chamber gas two is discharged in air, produces greenhouse
Effect, causes Global climate change.
CO in air2、CH4And N2O account for nearly 80% to the contribution rate of greenhouse effect, is the main contributions of greenhouse effect
Person.Wherein CO2Maximum to the contribution rate strengthening greenhouse effect, account for 56%, be most important greenhouse gases.Next to that CH4, its
Greenhouse effect potential is CO223 times, the contribution rate of greenhouse effect is accounted for 15%.According to estimates, air has 5%-20% every year
CO2, the CH of 15%-30%4, the N of 80%-90%2O derives from soil.And farmland is the releases of soil ecosystem greenhouse gases
Important sources.
The research produced for farmland greenhouse gas at present is a lot, but reports for work not for farmland greenhouse gas reduction of discharging
Many.Raw 2012 of Wu Hong patent of invention " 201210584476 " discloses the discharge-reducing method of a kind of rice field greenhouse gases, in rice field
Carry out alternation of wetting and drying, pour water and combine with dry field, reduce other technology of greenhouse, rice field.The raw patent of invention in 2013 of Wu Hong
" 201310750529.0 " introduce a kind of method that wheatland catch cropping Bulbus Allii reduces Soil Trace Gases, and a kind of Herba Alii fistulosi reduces farmland temperature
Room gas discharge method, it is characterised in that step is: a. is when rain fed crop is sowed or plant strain growth is broadcast to certain phase, mixing
Plant or transplant Herba Alii fistulosi: during autumn sowing, by 210cm mixed seeding onion seeds, Herba Alii fistulosi spacing in the rows 510cm at rain fed crop sowing, opening
Ditch is placed into the soil shallow for onion seeds, covers 13cm soil above, and is compacted；Or after rain fed crop emerges Second Year spring 3
Transplant Herba Alii fistulosi seedling the beginning of the month, transplant radius distance rain fed crop plant 210cm, spacing in the rows 510cm；B. sowing rain fed crop with big
During Herba Alii fistulosi mixed planting, in addition to normal fertilising, after Second Year Herba Alii fistulosi in spring emerges 25 days, or before transplanting Chinese Onion Seeding Field, to Herba Alii fistulosi
Seedling every mu uses 10 kilograms of Chemical Mixed Fertilizers and double centner fertilizer；In clear and bright one week after, Herba Alii fistulosi plant is used 10 kilograms of Chemical Mixed Fertilizers
With 50 kilograms of fertilizers；C. 2-chlorine 6-trichlorine to every mu of soil spraying concentration 0.2%wt-5%wt before and after Second Year the Ching Ming Festival
Picoline solution 50 kilograms, continues to spray the 2-chlorine 6-trichloromethyl pyridine solution 50 kilograms of same concentration after one and a half months；
D., after rain fed crop and Herba Alii fistulosi are gathered in the crops, stubble and the blade of Herba Alii fistulosi plant are pulverized, are uniformly sprinkled into field, and plough, pour water,
Make water consumpation saturated, maintain one so that the Herba Alii fistulosi plant residue of pulverizing is mixed homogeneously with agricultural land soil, decomposition, release
Go out antibacterial substance, then proceed to plant second stubble crop.
So far there is not yet the report using Antimicrobial agricultural land soil greenhouse gas emission.Microorganism is utilized to enter soil
The activity of suppression soil microorganism, thus reach to suppress the purpose of Soil Trace Gases, agricultural is reduced discharging, tackles climate change
There is important theory and practice meaning, have important application prospect.
Summary of the invention
It is an object of the invention to for above-mentioned deficiency of the prior art, it is provided that a kind of effectively minimizing agricultural land soil greenhouse gas
The microbial product of body discharge.
It is a further object of the present invention to provide a kind of simplicity, low cost, easy-operating effective minimizing agricultural land soil greenhouse gas
The preparation method of the microbial product of body discharge.
It is an object of the invention to be achieved through the following technical solutions:
The microbial product of a kind of effective minimizing agricultural land soil greenhouse gas emission, this microbial product is to mix at Semen Maydis vegetable garden soil
Compound feces adds brevibacterium frigoritolerans, bacillus amyloliquefaciens bank up 4-6 days after mixing thoroughly the product obtained, wherein, described jade
In rice vegetable garden soil mixture feces, Semen Maydis is 1:1 with the weight ratio of vegetable garden soil.
The preparation method of the microbial product of a kind of effective minimizing agricultural land soil greenhouse gas emission, comprises the following steps:
1) use liquid fermentation to produce brevibacterium frigoritolerans, obtain brevibacterium frigoritolerans fermentation centrifugal sediment；
2) use liquid fermentation to produce bacillus amyloliquefaciens, obtain bacillus amyloliquefaciens fermentation centrifugal sediment；
3) by weight 1:1, Semen Maydis powder and fertile vegetable garden soil are mixed, obtain Semen Maydis vegetable garden soil mixture feces；
4) in every 1000 kg corn powder, vegetable garden soil mixture, 20-200 kilogram of brevibacterium frigoritolerans fermentation is added respectively centrifugal heavy
Shallow lake thing, bacillus amyloliquefaciens fermentation centrifugal sediment, add 150-300 kg water, mix thoroughly rear plastic covering membrane stack put send out
Ferment 4-6 days, is the most effectively reduced the microbial product product of agricultural land soil greenhouse gas emission.
In the further design of the present invention, in step 2, the detailed process of liquid fermentation production brevibacterium frigoritolerans is:
Every 1000 kg water are added: soybean cake powder 5-15 kilogram, Semen Maydis powder 5-30 kilogram, 0.2-5 kilogram of magnesium sulfate, calcium carbonate
0.2-6 kilogram, 0.2-5 kilogram of zinc sulfate, regulate pH4.5-7.5, stir evenly 121 DEG C of sterilizings 35 minutes, be cooled to 30 DEG C by weight
Than inoculation 0.2-10% brevibacterium frigoritolerans, 30 DEG C of heat insulating culture, sweat stirred liquid 1-3 minute every 12-14 hour,
After cultivating 8-12 days continuously, centrifugal concentrating, obtain brevibacterium frigoritolerans fermentation precipitate, it is desirable to microscope inspection viable count is more than 5,000,000,000
Viable bacteria or spore/gram, standby.
In the further design of the present invention, in step 3, liquid fermentation produces the detailed process of bacillus amyloliquefaciens
For:
Every 1000 kg water are added: soybean cake powder 1-10 kilogram, Semen Maydis powder 5-60 kilogram, 0.2-4 kilogram of magnesium sulfate, calcium carbonate
0.2-5 kilogram, 0.2-5 kilogram of zinc sulfate, pH regulator, to 4.5-5.5, stirs evenly 121 DEG C of sterilizings 35 minutes, is cooled to 30 DEG C by weight
Amount, than inoculation 0.2-8% bacillus amyloliquefaciens, 30 DEG C of heat insulating culture, is stirred under the rotating speed of 90-130 rev/min, even
Continuous cultivate 36 hours after, centrifugal concentrating, obtain bacillus amyloliquefaciens fermentation precipitate, it is desirable to microscope more than 5,000,000,000 viable bacterias or
Spore/gram, standby.
The microbial product of above-mentioned effective minimizing agricultural land soil greenhouse gas emission is reducing agricultural land soil greenhouse gases
Producing and application in terms of discharge, this microorganism is used in every mu of this microbial product of paddy field application by throwing them 50-200 kilogram, dry farming field
80-400 kilogram of goods.
In the present invention, microorganism used therefor strain can be from Chinese Academy of Sciences's microorganism in one's power about colleges and universities, scientific research institution's culture presevation
Center obtains.
The present invention has a beneficial effect highlighted below:
The present invention has simplicity, low cost, easy-operating advantage, reduces discharging carrying for agricultural reply climate change and Agricultural greenhouse gas
For feasible operational approach and technology.In any one piece of farmland, before crop sowing or transplanting, while fertilising, execute for every mu
With double centner product of the present invention, it is possible to reduce discharge of methane 35-56%, reduce nitrous oxide emission 48-69%, can improve simultaneously
Crop yield 3-6%.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
1000 kg water are added: soybean cake powder 8 kilograms, Semen Maydis powder 10 kilograms, 0.5 kilogram of magnesium sulfate, calcium carbonate 1 kilogram, sulfur
1 kilogram of zinc of acid, pH is natural, stirs evenly 121 DEG C of sterilizings 35 minutes, be cooled to 30 DEG C by weight inoculation 0.5% brevibacterium frigoritoleranses, 30
DEG C heat insulating culture, every 12-14 hour agitation liquid 1 minute in sweat, after cultivating 8 days continuously, concentrates centrifugal, and it is heavy to collect
Shallow lake thing microscope inspection viable count is standby；
1000 kg water are added: soybean cake powder 2 kilograms, Semen Maydis powder 10 kilograms, 1 kilogram of magnesium sulfate, calcium carbonate 1 kilogram, sulphuric acid
1 kilogram of zinc, pH regulator to 4.5, stir evenly 121 DEG C of sterilizings 35 minutes, be cooled to 30 DEG C by weight inoculation 0.6% solution starch brood cell
Bacillus, 30 DEG C of heat insulating culture, start shaft, speed of agitator 100 revs/min, after cultivating 36 hours continuously, concentrate centrifugal, receive
Collection precipitate microscope inspection viable count is standby；
By weight 1:1, Semen Maydis powder and fertile vegetable garden soil are mixed, at 1000 kilograms of this Semen Maydis vegetable garden soil mixture feces
Add the above-mentioned brevibacterium frigoritolerans of double centner, bacillus amyloliquefaciens fermentation centrifugal sediment, add 150 kilograms of moisture, mix thoroughly,
Plastic covering film, banks up 4 days, product；
In paddy field, before shoot transplanting equipment, using this technical products 120 kilograms for every mu, comparison field is not used, and transplants
After rice shoot, use closed chamber method to gather a gas sample every 5 days, analyze methane and nitrous oxide with gas chromatograph, continuous 15
Secondary test greenhouse gases, the Oryza sativa L. field using this technical products reduces 43% than comparison field methane, and nitrous oxide reduces 56%,
Oryza sativa L. single-strain fresh weight increases by 12% than comparison field.
Embodiment 2
1000 kg water are added: soybean cake powder 15 kilograms, Semen Maydis powder 30 kilograms, 5 kilograms of magnesium sulfate, calcium carbonate 6 kilograms, sulphuric acid
5 kilograms of zinc, pH is natural, stirs evenly 121 DEG C of sterilizings 35 minutes, is cooled to 30 DEG C by weight inoculation 5% brevibacterium frigoritolerans, 30 DEG C of guarantors
Temperature is cultivated, and stirs liquid 3 minutes every 12-14 hour in sweat, after cultivating 8-12 days continuously, concentrates centrifugal, and collection is heavy
Shallow lake thing microscope inspection viable count is standby；
1000 kg water are added: soybean cake powder 10 kilograms, Semen Maydis powder 60 kilograms, 4 kilograms of magnesium sulfate, calcium carbonate 5 kilograms, sulphuric acid
5 kilograms of zinc, pH regulator to 5.5, stir evenly 121 DEG C of sterilizings 35 minutes, be cooled to 30 DEG C by weight inoculation 4% solution starch brood cell's bar
Bacterium, 30 DEG C of heat insulating culture, start shaft, speed of agitator 130 revs/min, after cultivating 36 hours continuously, concentrate centrifugal, collect
Precipitate microscope inspection viable count is standby；
By weight 1:1, Semen Maydis powder and fertile vegetable garden soil are mixed, at 1000 kilograms of this Semen Maydis vegetable garden soil mixture feces
Add 200 kilograms of above-mentioned brevibacterium frigoritoleranses, bacillus amyloliquefaciens fermentation centrifugal sediment, add 300 kilograms of moisture, mix thoroughly,
Plastic covering film, banks up 4-6 days, product；
In paddy field, before shoot transplanting equipment, using this technical products 180 kilograms for every mu, comparison field is not used, and transplants
After rice shoot, use closed chamber method to gather a gas sample every 5 days, analyze methane and nitrous oxide with gas chromatograph, continuous 15
Secondary test greenhouse gases, the Oryza sativa L. field using this technical products reduces 54% than comparison field methane, and nitrous oxide reduces
63%, Oryza sativa L. single-strain fresh weight increases by 14% than comparison field.
Being above presently preferred embodiments of the present invention, all changes made according to technical solution of the present invention, produced function is made
With during without departing from the scope of technical solution of the present invention, belong to protection scope of the present invention.
Claims (5)
1. the microbial product of an effective minimizing agricultural land soil greenhouse gas emission, it is characterised in that this microbial product is
In Semen Maydis powder, vegetable garden soil mixture feces, add brevibacterium frigoritolerans, bacillus amyloliquefaciens bank up 4-6 days after mixing thoroughly and to obtain
Product, wherein, in described Semen Maydis powder, vegetable garden soil mixture feces, Semen Maydis powder is 1:1 with the weight ratio of vegetable garden soil.
2. the preparation method of the microbial product effectively reducing agricultural land soil greenhouse gas emission described in claim 1, it is special
Levy and be, comprise the following steps:
1) use liquid fermentation to produce brevibacterium frigoritolerans, obtain brevibacterium frigoritolerans fermentation centrifugal sediment；
2) use liquid fermentation to produce bacillus amyloliquefaciens, obtain bacillus amyloliquefaciens fermentation centrifugal sediment；
3) by weight 1:1, Semen Maydis powder and fertile vegetable garden soil are mixed, obtain Semen Maydis powder vegetable garden soil mixture；
4) in every 1000 kg corn powder, vegetable garden soil mixture, 20-200 kilogram of brevibacterium frigoritolerans fermentation is added respectively centrifugal heavy
Shallow lake thing, bacillus amyloliquefaciens fermentation centrifugal sediment, add 150-300 kg water, mix thoroughly rear plastic covering membrane stack put send out
Ferment 4-6 days, is the most effectively reduced the microbial product product of agricultural land soil greenhouse gas emission.
The preparation method of the microbial product of effective minimizing agricultural land soil greenhouse gas emission the most according to claim 2,
It is characterized in that, in step 2, the detailed process of liquid fermentation production brevibacterium frigoritolerans is:
Every 1000 kg water are added: soybean cake powder 5-15 kilogram, Semen Maydis powder 5-30 kilogram, 0.2-5 kilogram of magnesium sulfate, calcium carbonate
0.2-6 kilogram, 0.2-5 kilogram of zinc sulfate, pH regulator, to 4.5-7.5, stirs evenly 121 DEG C of sterilizings 35 minutes, is cooled to 30 DEG C by weight
Amount, than inoculation 0.2-10% brevibacterium frigoritolerans, 30 DEG C of heat insulating culture, is divided every 12-14 hour agitation liquid 1-3 in sweat
Clock, after cultivating 8-12 days continuously, centrifugal concentrating, obtain brevibacterium frigoritolerans fermentation precipitate, it is desirable to microscope inspection viable count reaches
5000000000 viable bacterias/gram more than, standby.
The preparation method of the microbial product of effective minimizing agricultural land soil greenhouse gas emission the most according to claim 2,
It is characterized in that, in step 3, the detailed process of liquid fermentation production bacillus amyloliquefaciens is:
Every 1000 kg water are added: soybean cake powder 1-10 kilogram, Semen Maydis powder 5-60 kilogram, 0.2-4 kilogram of magnesium sulfate, calcium carbonate
0.2-5 kilogram, 0.2-5 kilogram of zinc sulfate, pH regulator, to 4.5-7.5, stirs evenly 121 DEG C of sterilizings 35 minutes, is cooled to 30 DEG C by weight
Amount, than inoculation 0.2-8% bacillus amyloliquefaciens, 30 DEG C of heat insulating culture, is stirred under the rotating speed of 90-130 rev/min, even
After continuous cultivation 36 hours, centrifugal concentrating, obtain bacillus amyloliquefaciens fermentation precipitate, it is desirable to microscope inspection viable count reaches 50
Hundred million viable bacterias/gram more than, standby.
5. the microbial product effectively reducing agricultural land soil greenhouse gas emission described in claim 1 is reducing agricultural land soil temperature
Application in terms of the generation of room gas and discharge, it is characterised in that every mu of this microbial product of paddy field application by throwing them 50-200 kilogram,
This microbial product 80-400 kilogram is used in dry farming field.
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| CN107969182A (en) * | 2017-09-15 | 2018-05-01 | 吴洪生 | It is a kind of to have ecological method that is disease-resistant and reducing the place greenhouse gas emissions such as farm and application concurrently |
| CN109964762A (en) * | 2019-04-26 | 2019-07-05 | 云南农业大学 | A kind of reduction Soil Trace Gases N2The cropping pattern of O discharge |
| WO2020076797A1 (en) * | 2018-10-09 | 2020-04-16 | Locus Ip Company, Llc | Materials and methods for enhanced carbon utilization and/or sequestration as well as reducing deleterious atmospheric gases |
| US11758924B2 (en) | 2019-04-12 | 2023-09-19 | Locus Solutions Ipco, Llc | Pasture treatments for enhanced carbon sequestration and reduction in livestock-produced greenhouse gas emissions |
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| US11758924B2 (en) | 2019-04-12 | 2023-09-19 | Locus Solutions Ipco, Llc | Pasture treatments for enhanced carbon sequestration and reduction in livestock-produced greenhouse gas emissions |
| CN109964762A (en) * | 2019-04-26 | 2019-07-05 | 云南农业大学 | A kind of reduction Soil Trace Gases N2The cropping pattern of O discharge |
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Application publication date: 20161026 |
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