CN103396258A - Inhibitor for methanogen and usage method thereof - Google Patents
Inhibitor for methanogen and usage method thereof Download PDFInfo
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- CN103396258A CN103396258A CN2013103618779A CN201310361877A CN103396258A CN 103396258 A CN103396258 A CN 103396258A CN 2013103618779 A CN2013103618779 A CN 2013103618779A CN 201310361877 A CN201310361877 A CN 201310361877A CN 103396258 A CN103396258 A CN 103396258A
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- solubilizing bacteria
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Abstract
The invention discloses an inhibitor for methanogen and a usage method thereof. The main components of the inhibitor are sterile water, compound microbial inoculum, dipotassium hydrogen phosphate, magnesium sulfate, and ammonium chloride. The mass ratio of the materials mentioned above is 80:2:6:4:8. The total bacteria number of the compound microbial inoculum in each milliliter is larger than 10 billion. The bacteria in the compound microbial inoculum are actinomyces, azotobacter, potassium bacteria, and phosphate solubilizing bacteria. The inhibitor for methanogen has the advantages as follows: first, the inhibitor can inhibit the biological activity of methanogen, prevents the methanogen from generating methanol and effectively protects the environment; secondly, the inhibitor can improve the micro-ecological conditions of the soil, inhibits and antagonizes the growth and living of other crop bacteria, and promotes the healthy and rapid growth of crops; thirdly, through more frequent microbial activities, the organic material content of the soil can be effectively improved, the contents of quick-acting nitrogen, phosphor and potassium are increased, and thus the fertility of soil is effectively improved.
Description
Technical field
The present invention relates to the inhibitor that a kind of methane produces bacterium, particularly relate to a kind of soil protection organic matter that has, reduce the generation of greenhouse gases, regulate soil micro-ecosystem, increase soil fertility, improve the conditioning products of crop yield and quality.
Background technology
In atmosphere by nature or the artificial gaseous constituent that can absorb long-wave radiation that produces.As steam (H
2O), carbonic acid gas (CO
2), Nitrous Oxide (N
2O), methane (CH
4), ozone (O
3) and Chlorofluorocarbons (CFCs) (CFC) be main greenhouse gas in earth atmosphere.Wherein carbonic acid gas and methane are greenhouse gases main in earth atmosphere, particularly methane, can not, by plant utilization, be difficult to again oxidized decomposition at nature, so more should attract great attention.Earth surface constantly produces carbonic acid gas and methane under the effect of various mushrooms, methanogen, climate change and impact thereof are multiple dimensioned, comprehensive, multi-level, and front and negative impact are also deposited, but negative impact is more concerned.Global warming has produced impact to the natural ecosystems in many areas, as climatic anomaly, sea-level rise, glacial recession, frozen soil melt, river (lake) ice freezes late with early melt, the middle high latitude season of growth extends, the animals and plants distribution range is extended to polar region and high Altitude Region, some animals and plants quantity reduces, some plant blossom times wait in advance.The increase of greenhouse gases is a more complicated problem on the impact of weather and the ecosystem, the increase of greenhouse gases will cause the variation of temperature and precipitation, can affect and change agroclimatic potential productivity, thereby change the bearing capacity of the ground of primary productivity and the agricultural of the ecosystem.According to the result of climatic simulation test, after greenhouse gases double, may cause torrid zone expansion, subtropics, the warm torrid zone and frigid zone dwindle, and cool temperature zone slightly increases, the area change of grassland and desert, the area of forest reduces.Greenhouse gases may have influence on the series of problems such as planting decision-making, kind layout and breed improvement, soil utilization, agricultural input and technological improvement of agricultural.Therefore when the long-term planning of the development strategy of formulating country and agricultural, should consider weather that Increasing in greenhouse gases may cause and the variation background of environment.Secondly, the carcinogenesis of human of greenhouse gases will be to consume a large amount of soil organism, reduce soil productivity, have a strong impact on output and the quality of crop product.This seems particularly important and urgent for the China that faces population expansion and per capita resources poor two large pressure.
Summary of the invention
The object of the present invention is to provide a kind of methane to produce inhibitor and the using method thereof of bacterium, it can effectively reduce the discharging of greenhouse gases, increases soil fertility, effectively improves unit surface crop yield and quality.Methane produces the Physiology and biochemistry conditional request of bacteria inhibitor according to methanogen, by the great many of experiments method, obtains, and can prevent that methanogen from producing methane; by the microorganism species interphase interaction; can effectively reduce the discharging of carbonic acid gas, protection of the environment, preserve our planet.Improve the soil micro-ecosystem condition, growth, the survival of inhibition, other crop germ of antagonism, promote that crop is healthy, Fast Growth., by a large amount of microbial activitiess, can effectively improve soil organic matter content, increase the content of Soil Available nitrogen phosphorus potassium, thereby effectively increase soil fertility.Reduce the loss of the soil organism, play fixed nitrogen and release the effect of the fertile fertilizer conservation of fattening.Fill up prior art blank, provide a kind of brand-new mankind's protection of the environment, the effective ways of preserving our planet, with minimum cost, effective means; reach best, maximum effect; serve the mankind,, for the earth escorts, accelerate the development of human society.
Technical scheme of the present invention is such realization: the main component that described methane produces the inhibitor of bacterium is composite fungus agent, inorganic salt and sterilized water; Described sterilized water, for not containing the water of other miscellaneous bacteria, adds composite fungus agent and dipotassium hydrogen phosphate, sal epsom, ammonium chloride in water, its component ratio is 80: 2: 6: 4: 8; Composite fungus agent is four kinds, actinomycetes, azotobacter, potassium solubilizing bacteria, phosphate solubilizing bacteria, composite fungus agent always contain every milliliter of bacterium number greater than 100 hundred million.
Described composite fungus agent is combined by actinomycetes, azotobacter, potassium solubilizing bacteria, phosphate solubilizing bacteria; Its composition 2 content are respectively: actinomycetes 0.6, azotobacter 0.6, potassium solubilizing bacteria 0.4, phosphate solubilizing bacteria 0.4.Different types of bacterium and inorganic salt are combined into methane and produce bacteria inhibitor, can effectively reduce the generation that even prevents the field methane gas, also are improved simultaneously the effect of soil productivity.
After 200 times of described inhibitor thin ups, be sprayed in paddy field, nonirrigated farmland or wetland, by cultivation process, inhibitor is uniformly distributed in the ground, after one month, then spray 1-3 time.
Described compound probiotic is cultivated respectively, purifying and composite acquisition.
compared with prior art, the present invention has the following advantages: at present, although there are many scholars or to study the methanogen associated problem, but be also the research at the aspects such as growth aerogenesis rule of carrying out methanogen mostly, be also in exploratory study for the discharging that how effectively to reduce methane gas, substantially be in a kind of response state of passiveness, also do not find that there is direct effective inhibitor for methanobacteria, prove that by experiment this inhibitor can not only effectively suppress the biological activity of methanogen, greatly reduce the generation that suppresses methane gas even fully, can make the discharging of methane gas volume reduce 80-100%, and can effectively increase soil fertility, improve soil microenvironment.
Embodiment
The invention will be further described below in conjunction with embodiment:
The main component that described methane produces the inhibitor of bacterium is composite fungus agent, inorganic salt and sterilized water; Described sterilized water, for by sterilizing water, adds composite fungus agent and inorganic salt in sterilized water; Its quality component ratio is 80: 20; Described composite fungus agent always contain every milliliter of bacterium number greater than 100 hundred million; Mushroom in described compound probiotic is actinomycetes, azotobacter, potassium solubilizing bacteria, phosphate solubilizing bacteria, and described inorganic salt are dipotassium hydrogen phosphate, sal epsom, ammonium chloride.
Described compound probiotic carries out respectively culture purified and composite.
In following examples, sterilized water adopts sterilising method to obtain, and it is 80 that the quality proportioning is sterilized water, and composite fungus agent is 2, and inorganic salt are 18.
In following examples, using method is: directly spray application in soil by diluting, act on every mu, inhibitor that prerequisite is executed and be no less than 5.0L.
Methane produces bacteria inhibitor embodiment 1: paddy field
When spring ploughing, after 200 times of described inhibitor thin ups, be sprayed at field, by cultivation process, inhibitor is evenly distributed in paddy field.After one month, then spray 1-2 time.
Mushroom in composite fungus agent is actinomycetes azotobacter, potassium solubilizing bacteria, phosphate solubilizing bacteria, and described inorganic salt are dipotassium hydrogen phosphate, sal epsom, ammonium chloride.
Result of use: by adding the effective ingredients such as external beneficial microorganism; can improve the paddy field soil micro-ecological environment; antagonism suppresses methanogen; reduce the biological activity of methanogen, greatly reduce and even suppress methanogen generation methane, make methane gas volume burst size reduce 80-100%; effectively organic consumption of protection decomposed; accelerate to improve the soil beneficial microorganism and exist, increase the soil nutrient composition, promote the healthy Fast Growth of crop.
Methane produces the embodiment 2 of bacteria inhibitor: non-irrigated native vegetable plot
When nonirrigated farmland is cultivated, after 200 times of described inhibitor thin ups, be sprayed in vegetable plot, by cultivation process, the methane inhibitor is evenly distributed in soil.After one month, then spray 2-3 time, effect is better.
Mushroom in composite fungus agent is actinomycetes, azotobacter, potassium solubilizing bacteria, phosphate solubilizing bacteria, and described inorganic salt are dipotassium hydrogen phosphate, sal epsom, ammonium chloride.
Result of use: by adding external beneficial microorganism, improve the nonirrigated farmland micro-ecological environment, antagonism suppresses methanogen, harmful microbe is grown, reduce the biological activity of methanogen, greatly reduce and even suppress methanogen generation methane, make methane gas volume burst size reduce 95-100%, effectively the organic consumption of protection is decomposed, effectively protect the quick-acting nutritive elements such as nitrogen phosphorus potassium, prevent their loss; Accelerate to improve the soil beneficial microorganism and exist, increase the soil nutrient composition, promote the healthy Fast Growth of crop, improve nutrition and the quality of crop.
Methane produces the embodiment 3 of bacteria inhibitor: wetland
After 200 times of described inhibitor thin ups, be sprayed in wetland, by effects such as current, inhibitor is evenly distributed in soil voluntarily.After one month, then add and spray 1-2 time, effect is better.
Mushroom in composite fungus agent is actinomycetes azotobacter, potassium solubilizing bacteria, phosphate solubilizing bacteria, and described inorganic salt are dipotassium hydrogen phosphate, sal epsom, ammonium chloride.
Result of use: by adding external beneficial microorganism; improve the wetland micro-ecological environment; antagonism suppresses methanogen; reduce the biological activity of methanogen; greatly reduce and even suppress methanogen generation methane; make methane gas volume burst size reduce 90-100%; effectively organic consumption of protection decomposed; accelerating to improve the soil beneficial microorganism exists; increase the soil nutrient composition; eliminate the genesis of Plant diseases, promote Wetland Biodiversity fast-developing, guarantee normal, health and the efficient organic circulation of the ecosystem in wetland.
The present invention has the following advantages:
1 environmental protection, pollution-free: this product mainly utilizes the knowledge of crop alimentary, microbial nutrition and microorganism, by the Effective Regulation to soil micro-ecosystem composition, microbial nutrition, the physiologically active that suppresses the floras such as methanogen, thereby the generation of inhibition methane isothermal chamber gas.
2 effective, timeliness is long: form by changing soil microorganisms, improve soil microenvironment, suppress, the growth of antagonism Methane in Soils bacterium and germ, make adding flora become dominant microflora, the group but the long term keeps on top, form more stable holddown.
3 can reduce the generation of greenhouse gases, can also improve soil productivity, increase the soil nutrient composition, promote crop yield and quality.
4 by improving soil microenvironment, and the quick-acting nutritive elements such as protection nitrogen phosphorus potassium, prevent their loss.
5 by improving soil microenvironment, eliminates the genesis of Plant diseases, and effectively protective plant grows.
6 solid carbon: prevent the digested utilization of carbon in soil with organic matter, run out of with the form of carbonic acid gas, thereby the maintenance of soil productivity is had vital role.
Claims (3)
1. a methane produces inhibitor and the using method thereof of bacterium, and its feature: described composite inhibitor is comprised of sterilized water, composite fungus agent, dipotassium hydrogen phosphate, sal epsom, ammonium chloride; Its component ratio is 80: 2: 6: 4: 8; Composite fungus agent is four kinds, actinomycetes, azotobacter, potassium solubilizing bacteria, phosphate solubilizing bacteria, composite fungus agent always contain every milliliter of bacterium number greater than 100 hundred million.
2. a kind of methane according to claim 1 produces bacteria inhibitor and using method thereof, it is characterized in that described composite fungus agent is combined by actinomycetes, azotobacter, potassium solubilizing bacteria, phosphate solubilizing bacteria; Its content is respectively: actinomycetes 0.6, azotobacter 0.6, potassium solubilizing bacteria 0.4, phosphate solubilizing bacteria 0.4.
3. a kind of methane according to claim 1 produces bacteria inhibitor and using method thereof, it is characterized in that being sprayed in paddy field, nonirrigated farmland or wetland after 200 times of described inhibitor thin ups, by cultivation process, inhibitor is uniformly distributed in the ground, after one month, then spray 1-3 time.
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| CN201310361877.9A CN103396258B (en) | 2013-08-20 | 2013-08-20 | A kind of inhibitor of methane producing strains and using method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106045772A (en) * | 2016-05-31 | 2016-10-26 | 南京信息工程大学 | Microorganism product capable of effectively reducing greenhouse gas emission of farmland soil and preparation method of microorganism product |
| CN115298287A (en) * | 2020-04-21 | 2022-11-04 | 组合化学工业株式会社 | Methane formation inhibitor composition and method for inhibiting methane formation |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1090707A (en) * | 1992-10-22 | 1994-08-17 | 生物技术资源两合公司 | Anthraquinone produces the inhibitory action that bacterium produces methane to methane |
| KR20060019062A (en) * | 2004-08-26 | 2006-03-03 | 진주산업대학교 산학협력단 | Feed ingredients against methane gas produced by the rumen of ruminant animals |
| CN1896258A (en) * | 2006-06-28 | 2007-01-17 | 江南大学 | Oriented production of acetic acid in sludge anaerobic fermentation by methane-bacterium specific inhibitor |
| KR20090129786A (en) * | 2008-06-13 | 2009-12-17 | 한국과학기술연구원 | Method and apparatus for bio-hydrogen gas production from organic waste by using inhibitor for methane producing microorganisms and gas purging under low ph condition |
| CN102633366A (en) * | 2012-05-03 | 2012-08-15 | 重庆大学 | Mountainous urban artificial wetland sewage treatment system capable of controlling methane emission |
| US20130129684A1 (en) * | 2010-05-18 | 2013-05-23 | Able Corporation | Method of inhibiting methanogenesis |
-
2013
- 2013-08-20 CN CN201310361877.9A patent/CN103396258B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1090707A (en) * | 1992-10-22 | 1994-08-17 | 生物技术资源两合公司 | Anthraquinone produces the inhibitory action that bacterium produces methane to methane |
| KR20060019062A (en) * | 2004-08-26 | 2006-03-03 | 진주산업대학교 산학협력단 | Feed ingredients against methane gas produced by the rumen of ruminant animals |
| CN1896258A (en) * | 2006-06-28 | 2007-01-17 | 江南大学 | Oriented production of acetic acid in sludge anaerobic fermentation by methane-bacterium specific inhibitor |
| KR20090129786A (en) * | 2008-06-13 | 2009-12-17 | 한국과학기술연구원 | Method and apparatus for bio-hydrogen gas production from organic waste by using inhibitor for methane producing microorganisms and gas purging under low ph condition |
| US20130129684A1 (en) * | 2010-05-18 | 2013-05-23 | Able Corporation | Method of inhibiting methanogenesis |
| CN102633366A (en) * | 2012-05-03 | 2012-08-15 | 重庆大学 | Mountainous urban artificial wetland sewage treatment system capable of controlling methane emission |
Non-Patent Citations (2)
| Title |
|---|
| 刘小雪，等: "农田甲烷产生的微生物生态学机理研究进展", 《上海农业科技》 * |
| 王维奇，等: "控制湿地甲烷产生的主要电子受体研究进展", 《地理科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106045772A (en) * | 2016-05-31 | 2016-10-26 | 南京信息工程大学 | Microorganism product capable of effectively reducing greenhouse gas emission of farmland soil and preparation method of microorganism product |
| CN115298287A (en) * | 2020-04-21 | 2022-11-04 | 组合化学工业株式会社 | Methane formation inhibitor composition and method for inhibiting methane formation |
| CN115298287B (en) * | 2020-04-21 | 2024-05-14 | 组合化学工业株式会社 | Methanogenesis inhibitor composition and method for inhibiting methanogenesis |
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