CN106036080B - A kind of methane adjusting control agent and its application containing Candida tropicalis - Google Patents

A kind of methane adjusting control agent and its application containing Candida tropicalis Download PDF

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CN106036080B
CN106036080B CN201610399741.0A CN201610399741A CN106036080B CN 106036080 B CN106036080 B CN 106036080B CN 201610399741 A CN201610399741 A CN 201610399741A CN 106036080 B CN106036080 B CN 106036080B
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methane
vitamin
intermixture
feed
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CN106036080A (en
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刁其玉
屠焰
邓凯东
肖怡
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Feed Research Institute of Chinese Academy of Agricultural Sciences
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Feed Research Institute of Chinese Academy of Agricultural Sciences
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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    • Y02P60/22—Methane [CH4], e.g. from rice paddies

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Abstract

The methane adjusting control agent and its application, the methane adjusting control agent that the present invention relates to a kind of containing Candida tropicalis are made of candida tropicalis, calcium chloride, ammonium sulfate and lactose.The application is application of the methane adjusting control agent in preparation ruminant methane emission reduction feed.Inventor develops methane adjusting control agent, in formula, other than playing the Candida tropicalis of main function, increase lactose, ammonium sulfate and calcium chloride, the wherein nutritional supplement of lactose, ammonium sulfate as Candida tropicalis, calcium chloride is as its activator, to improve the survival and reproduction ability of candida tropicalis.By surveying 24 hours carbon dioxide of mutton sheep, methane emission, confirm that the technology of the present invention can reduce Methane Emission from Ruminants to a certain extent, improve lumen fermentation performance, improve lumen fermentation type, improve the capacity usage ratio of feed, and nutritive digestibility still can be improved, it is better than other methane inhibitors.

Description

A kind of methane adjusting control agent and its application containing Candida tropicalis
Technical field
The present invention relates to ruminants to cultivate field, and in particular to can reduce the premixed feed of cattle and sheep methane emission.
Background technique
Ruminant tumor gastric is a dynamic ecosystem, methane (CH4) generation be a complicated process, be The inevitable product of carbohydrate fermentation in cud.However the discharge of methane can reduce efficiency of feed utilization, aggravation greenhouse effect It answers, therefore, it is necessary to be regulated and controled using scientific and rational measure to it.
Currently, the research about the regulation of cud methane is existing very much.And probiotics are as novel green additive, To the Small side effects of animal and environment, the application in ruminant production focuses mostly in growth promotion, anti-disease, increases productivity Energy etc. to be had been reported that, but seldom sees to regulate and control for methane.
Therefore, suitable micro-ecological additive and its scientific and rational additive capacity are found to reduce ruminant methane Discharge amount have critically important research and application value.
Blastocystis in probiotics is used as feed additive for ruminant, not only in native fungal substance It can promote the healthy aquaculture of animal, while also comply with the requirement of animal product consumer, obtained in ruminant feed wide General application.A large number of studies show that addition saccharomycete has active influence to ruminant production.Saccharomycete is in ruminant Cud CH4Discharge in terms of have inhibiting effect.When adding yeast culture in barley Basic drawing, 12 after discovery feeding ~ for 24 hours CH in interior cud4Yield just decreases (Mutsvangwa, 1992(The effect of dietary inclusion of yeast culture (Yea-Sacc) on patterns of rumen fermentation, food intake and Growth of intensively fed bulls [J] Anim Prod, 1992,55: 35-40.))；Qiao Guohua etc. (2006) the cud CH of milk cow is reduced using the method discovery candida tropicalis culture of short-term artificial rumen fermentation4It produces Amount.But because in vitro method can not simulate the true ruminal environment of animal body, the dosage obtained is difficult to be directly used in production reality.
Inventor in practice, it has been found that Direct-fed Candida tropicalis reduce discharge of methane effect it is unstable, be Increase its effect, inventor has carried out a large amount of test, final to propose the present invention.
Summary of the invention
The object of the present invention is to provide can reduce Methane Emission from Ruminants and improve the pre- of utilization of nutrients Mixed fodder.
The first aspect of the present invention is to provide a kind of methane adjusting control agent containing Candida tropicalis, by tropical false silk ferment Mother, calcium chloride, ammonium sulfate, lactose composition.
Specifically, the methane adjusting control agent is composed of the following components in parts by weight: 8-15 parts of Candida tropicalis, chlorination 10-20 parts of calcium, 20-60 parts of ammonium sulfate, 20-60 parts of lactose, Candida tropicalis (Candida tropicalis) viable bacteria list Position 5 × 109CFU/g；
Preferably, the methane adjusting control agent is composed of the following components in parts by weight: 8-10 parts of candida tropicalis, calcium chloride 15-20 parts, 20-40 parts of ammonium sulfate, 20-40 parts of lactose；
Preferably, the methane adjusting control agent is composed of the following components in parts by weight: 8-10 parts of candida tropicalis, calcium chloride 15-20 parts, 20-35 parts of ammonium sulfate, 20-35 parts of lactose；
Still more preferably, the methane adjusting control agent is composed of the following components in parts by weight: 10 parts of candida tropicalis, chlorine Change 20 parts of calcium, 35 parts of ammonium sulfate, 35 parts of lactose.
In above-mentioned methane adjusting control agent:
The parts by weight can be unit of weight well known to the field of medicaments such as μ g, mg, g, kg, be also possible to its multiple, such as 1/2,1/3,1/10,1/100,2 times, 3 times, 4 times, 5 times, 6 times, 10 times, 100 times etc..
Candida tropicalis (Candida tropicalis) it is any candida tropicalis.
The present invention also provides the preparation method of above-mentioned methane adjusting control agent, this method is to be uniformly mixed four kinds of ingredients, i.e., It can.
Second aspect of the present invention is to provide application of the above-mentioned methane adjusting control agent in preparation ruminant methane emission reduction feed.
In the application:
The feed, contains following component: methane adjusting control agent, vitamin intermixture, minerals intermixture and carrier.
Specifically, the feed contains the ingredient of following parts by weight: 5-10 parts of methane adjusting control agent, vitamin intermixture 6-20 Part, 40-80 parts of minerals intermixture, 8.5-38 parts of carrier, the carrier are maize cob meal；
Preferably, the ingredient of following parts by weight: 8-10 parts of methane adjusting control agent, vitamin intermixture is contained in the feed 12-20 parts, 40-54 parts, -38 parts of carrier 18 of minerals intermixture；
Further preferably, the ingredient of following parts by weight: 8 parts of methane adjusting control agent, vitamin intermixture is contained in the feed 20 parts, 54 parts of minerals intermixture, carrier 18 part.
In above-mentioned feed:
Every part of vitamin intermixture is to be composed of the following components in parts by weight: 1.2-4 parts of vitamin A, vitamin D 0.2-0.5 parts, 3-10 parts of vitamin E, 85-95 parts of carrier, the carrier are maize cob meal；
Preferably, every part of vitamin intermixture is to be composed of the following components in parts by weight: vitamin A 1.2-2.0 Part, 0.2-0.5 parts of vitamin D, 3.6-6 parts of vitamin E, 91-95 parts of carrier；
Further preferably, every part of vitamin intermixture is composed of the following components in parts by weight: 1.2 parts of vitamin A, 0.2 part of vitamin D, 3.6 parts of vitamin E, 95 parts of carrier.
Every part of minerals intermixture is composed of the following components in parts by weight: 5-10 parts of ferrous sulfate monohydrate, five water sulphur Sour copper 0.8-2.0 parts, 5-10 parts of manganese sulfate monohydrate, 5-10 parts of monohydrate zinc sulphate, 0.8-2 parts of the cobalt chloride containing cobalt 1%, contain iodine 1% 2-4 parts of potassium iodide, 0.5-1 parts of sodium selenite containing selenium 1%, 61-81 parts of carrier, the carrier be maize cob meal, zein Powder or powdered rice hulls；
Preferably, every part of minerals intermixture is composed of the following components in parts by weight: ferrous sulfate monohydrate 7.6-10 Part, 1.2-2.0 parts of cupric sulfate pentahydrate, 7.3-10 parts of manganese sulfate monohydrate, 7.3-10 parts of monohydrate zinc sulphate, the cobalt chloride containing cobalt 1% 1.3-2 parts, 3.5-4 parts of the potassium iodide containing iodine 1%, 0.8-1 parts of sodium selenite containing selenium 1%, 61-71 parts of carrier；
Further preferably, every part of mineral complex is composed of the following components in parts by weight: ferrous sulfate monohydrate 7.6 Part, 1.3 parts of the cobalt chloride containing cobalt 1%, contains iodine 1% at 1.2 parts of cupric sulfate pentahydrate, 7.3 parts of manganese sulfate monohydrate, 7.3 parts of monohydrate zinc sulphate 3.5 parts of potassium iodide, 0.8 part of sodium selenite containing selenium 1%, 71 parts of carrier.
The carrier is maize cob meal, corn protein powder, powdered rice hulls, preferably maize cob meal.
There is provided the preparation methods of above-mentioned feed for third aspect present invention, method includes the following steps:
Each component is weighed according to the proportion, then prepares methane adjusting control agent, vitamin intermixture, minerals intermixture respectively, Carrier is weighed according to the ratio again, then according still further to all the components in equivalent gradually-increased mixing premixed feed.
The present invention also provides above-mentioned premixed feed inhibit methane discharge of ruminant feed application.
The application, which refers to, claims above-mentioned premixed feed with the ratio that the 0.5-5% of fully mixed diet is preferably 0.5-2% After amount, the water of 1 times of amount is added, stirs evenly, places preferred 4-7h of 3-10h or so at room temperature, be added in fully mixed diet, It after mixing, can feeding ruminants.The amount of wherein carrier can also be zoomed in or out, to zoom in or out above-mentioned premix Close ratio of the feed in fully mixed diet.
The fully mixed diet is to be made of (dry matter basis) following parts by weight ingredient: 50 ~ 70 parts of sheep's hay, corn 17 ~ 29 parts, 6 ~ 10 parts of dregs of beans, 1.0 ~ 2.0 parts of calcium monohydrogen phosphate, 0.3 ~ 0.5 part of mountain flour, 0.2 ~ 0.8 part of salt, premixed feed 0.5 ~ 1 Part.
Containing the yeast premixed feed provided by the invention for reducing methane discharge of ruminant has the advantage that
1, the document in the prior art such as background technique is to give ruminant Direct-fed Candida tropicalis, and the torrid zone is false Silk yeast cells can be rehydrated after entering cud, and carries out active metabolic activity.Yeast can not be raised directly for animal digestion Material, only when yeast cells is hydrolyzed, the benefit for feeding viable yeast can be from nutrition provided by yeast cell contents It is embodied on ingredient.Viable yeast can not in cud mass propagation, only a kind of transitional organism, in cud anaerobism ring Grow very slow in border；Meanwhile in cud various bacteriums contention sugars competition it is very fierce, this competition objectively makes Yeast is difficult to obtain nutrient appropriate.Therefore, Direct-fed Candida tropicalis cannot play Candida tropicalis completely Reduction discharge of methane curative effect.
Based on this, the methane adjusting control agent that inventor develops, in formula, the false silk ferment in the torrid zone in addition to playing main function Outside female bacterium, lactose, ammonium sulfate and calcium chloride are increased, wherein the nutritional supplementation of lactose, ammonium sulfate as Candida tropicalis Agent, calcium chloride is as its activator, to improve the survival and reproduction ability of candida tropicalis.
2, inventor abandons the defect of in vitro test, the method for surveying methane emission by respiratory metabolism test method, It filters out effective methane adjusting control agent and containing its feed, is applied to ruminant aquaculture, reduce discharge of methane level, improve life Produce economic benefit.
3, by surveying 24 hours carbon dioxide of mutton sheep, methane emission, it was demonstrated that the technology of the present invention can be to a certain degree Upper reduction Methane Emission from Ruminants improves lumen fermentation performance, improves lumen fermentation type, improves the energy utilization of feed Rate, and nutritive digestibility still can be improved, it is better than other methane inhibitors.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
The candida tropicalis, viable bacteria unit 5 × 109CFU/g is purchased from Angel Yeast joint-stock company；
Other feed addictives and feedstuff are all obtained from feed market purchase.
Embodiment 1: the composition and preparation method of methane adjusting control agent
Candida tropicalis 10kg, calcium chloride 20kg, ammonium sulfate 35kg, lactose 35kg are weighed, is uniformly mixed, it is spare.
Embodiment 2: the composition and preparation method of methane adjusting control agent
Candida tropicalis 8kg, calcium chloride 10kg, ammonium sulfate 20kg, lactose 20kg are weighed, is uniformly mixed, it is spare.
Embodiment 3: the composition and preparation method of methane adjusting control agent
Candida tropicalis 15kg, calcium chloride 15kg, ammonium sulfate 60kg, lactose 60kg are weighed, is uniformly mixed, it is spare.
A kind of embodiment 4: ruminant methane emission reduction feed
1, it forms:
Composition Weight (kg) Weight percent (%)
Methane adjusting control agent (method of embodiment 1 is prepared) 80 8
Vitamin intermixture 200 20
Minerals intermixture 540 54
Maize cob meal 180 18
It is total 1000 100
Wherein:
1) composition of the vitamin intermixture are as follows:
Composition Weight (kg) Weight percent (%)
Vitamin A 1.2 1.2
Vitamin D 0.2 0.2
Vitamin E 3.6 3.6
Maize cob meal 95 95
It is total 100 100
Preparation method: after mixing according to equivalent gradually-increased, spare by the ingredient；
2) composition of the minerals intermixture are as follows:
Composition Weight (kg) Weight percent (%)
Ferrous sulfate monohydrate 7.6 7.6
Cupric sulfate pentahydrate 1.2 1.2
Manganese sulfate monohydrate 7.3 7.3
Monohydrate zinc sulphate 7.3 7.3
Cobalt chloride containing cobalt 1% 1.3 1.3
Potassium iodide containing iodine 1% 3.5 3.5
Sodium selenite containing selenium 1% 0.8 0.8
Maize cob meal 71 71
It is total 100 100
Each raw material is weighed according to the proportion, it is spare after mixing.
2, preparation method:
Each raw material is weighed according to the proportion, after mixing to get methane emission reduction feed.
A kind of embodiment 5: ruminant methane emission reduction feed
1, it forms:
Composition Weight (kg) Weight percent (%)
Methane adjusting control agent (prepared by the method for embodiment 2) 100 10
Vitamin intermixture 120 12
Minerals intermixture 400 40
Maize cob meal 380 38
It is total 1000 100
Wherein:
1) composition of the vitamin intermixture are as follows:
Composition Weight (kg) Weight percent (%)
Vitamin A 2.0 2.0
Vitamin D 0.5 0.5
Vitamin E 6.0 6.0
Maize cob meal 91.5 91.5
It is total 100 100
Preparation method: after mixing according to equivalent gradually-increased, spare by the ingredient；
2) composition of the minerals intermixture are as follows:
Composition Weight (kg) Weight percent (%)
Ferrous sulfate monohydrate 10 10
Cupric sulfate pentahydrate 2 2
Manganese sulfate monohydrate 10 10
Monohydrate zinc sulphate 10 10
Cobalt chloride containing cobalt 1% 2 2
Potassium iodide containing iodine 1% 4 4
Sodium selenite containing selenium 1% 1 1
Corn protein powder 61 61
It is total 100 100
Each raw material is weighed according to the proportion, it is spare after mixing.
2, preparation method:
Each raw material is weighed according to the proportion, after mixing to get methane emission reduction feed.
A kind of embodiment 6: ruminant methane emission reduction feed
1, it forms:
Composition Weight (kg) Weight percent (%)
Methane adjusting control agent (prepared by the method for embodiment 3) 55 5.5
Vitamin intermixture 60 6
Minerals intermixture 800 80
Maize cob meal 85 8.5
It is total 1000 100
Wherein:
1) composition of the vitamin intermixture are as follows:
Composition Weight (kg) Weight percent (%)
Vitamin A 4 4
Vitamin D 0.4 0.4
Vitamin E 10 10
Maize cob meal 85.6 85.6
It is total 100 100
Preparation method: after mixing according to equivalent gradually-increased, spare by the ingredient；
2) composition of the minerals intermixture are as follows:
Composition Weight (kg) Weight percent (%)
Ferrous sulfate monohydrate 5 5
Cupric sulfate pentahydrate 0.8 0.8
Manganese sulfate monohydrate 5 5
Monohydrate zinc sulphate 5 5
Cobalt chloride containing cobalt 1% 0.8 0.8
Potassium iodide containing iodine 1% 2 2
Sodium selenite containing selenium 1% 0.5 0.5
Powdered rice hulls 80.9 80.9
It is total 100 100
Each raw material is weighed according to the proportion, it is spare after mixing.
2, preparation method:
Each raw material is weighed according to the proportion, after mixing to get methane emission reduction feed.
Experimental example 1:
1. test material and equipment
Candida tropicalis, viable bacteria unit 5 × 109CFU/g is purchased from Angel Yeast joint-stock company.
Methane surveys device: Open path gas metabolic system, LGR gas analyzer are purchased from Sable company, the U.S..It is a whole set of Experimental rig is made of the measurement device of Sable system, closed gas respiratory chamber and matched computer software.It once can be same When measure 6 sheep whole day breathing gas and methane emission.
2. experimental animal and design
It is designed using single factor test completely randomized experiment.Choosing 30 weight is (51.12 ± 2.23) kg, and the age is close, body The good Dorper sheep of condition (♂) × Small-fat-tail sheep (♀) first familiar generation is grown up wether, is divided into 5 processing groups, i.e. 1 control group and 4 test groups, every group of 6 sheep, every sheep are 1 repetition, and each group feeds the fully mixed diet of same recipe, wherein methane emission reduction Feed is respectively as follows:
A group: control group, using fully mixed diet, no methane emission reduction feed；
B group: the methane emission reduction feed provided using embodiment 4；
C group: the methane emission reduction feed provided using embodiment 4, wherein methane adjusting control agent is the formula that embodiment 2 provides；
D group: the methane emission reduction feed provided using embodiment 4, wherein methane adjusting control agent is the formula that embodiment 3 provides；
E group: Direct-fed Candida tropicalis (adds 6g/100kg, with vacation silk in the torrid zone in B group in fully mixed diet Inoculum of dry yeast is similar), other compositions are not added, fully mixed diet meets the requirement of table 1.
By the prepared methane emission reduction feed of B, C, D group, is weighed in 1% ratio of fully mixed diet, be added into 1 Times water, places 6h after mixing evenly, is added in fully mixed diet and is uniformly mixed, can feed to ruminants such as cattle and sheep.
31 d of experimental period, wherein 18 d of preliminary trial period, positive to try 13 d of phase.
3. testing daily ration: for fine fodder and sheep's hay, fine fodder is shown in Table 1.Sheep's hay is prescinded to be mixed with fine fodder according to the ratio of table 1 afterwards.
1 fully mixed diet of table forms (%, dry matter basis)
Project-raw material Content
Sheep's hay 64.6
Corn 26.3
Dregs of beans 6.1
Calcium monohydrogen phosphate 1.25
Mountain flour 0.5
Salt 0.25
Premix 1.0
It is total 100.00
Note: E group tests premix in daily ration and is purchased from Beijing Precise Animal Nutrient Research Center, provides for every kilogram of fine fodder: 1.2 ten thousand IU of vitamin A, vitamin D 3000IU, vitamin E 70g, vitamin B1 0.3mg, vitamin B2 0.5mg, niacin 0.3mg；Lysine 22g, methionine 12g, tryptophan 2.8g；Iron 90mg, copper 12mg, zinc 110mg, manganese 120mg, selenium 0.3mg, iodine The micro constitutents such as 1.3mg, cobalt 0.5mg.B, C, D group are with premix in 4 substitution tables of embodiment, and A group is then without the false silk ferment in the torrid zone Premix in 4 substitution tables of embodiment of female bacterium.
4. testing index and method
4.1 test sheep weight
When carrying out gaseous metabolism test, weight survey is carried out to it respectively when testing sheep and entering and leaving gaseous metabolism room It is fixed, calculation basis of the average weight measured twice as test sheep metabolic body size.Metabolic body size (W0.75)=average weight0.75。
4.2 CH4Discharge amount
CH4Discharge amount is measured using Open path gas metabolic system (Sable, USA), and system connects 6 breathings and surveys Hot tank can measure the CH of 6 sheep simultaneously4Discharge amount.It is furnished with hopper and sink in each breath heat measurement case, test sheep is being tested Period can be freely eaten and drink water.After test sheep enters breath heat measurement case, 24 h are first adapted to, then measure the CH of 48 h4Discharge Amount.Measuring the system circulation primary time is 60 min.When starting measurement, system measures CH in experimental enviroment first4Content, Minute is 2 min, is then replaced from environment into breath heat measurement case, and time swap is 1 min, then sequentially determining 6 The CH of breath heat measurement case4Discharge amount, each breath heat measurement case minute are 9 min, and then system is from breath heat measurement case to ring Border displacement, time swap are 1 min, finally measure the CH in experimental enviroment again4Content, minute are 2 min.The above are Sable Open path gas metabolic system completes the measurement process of one cycle, and the CH of 48 h of METHOD FOR CONTINUOUS DETERMINATION is recycled with this4Discharge Amount.In calculating process, with the CH measured in experimental enviroment twice4The average value of content passes through system Sable gas as substrate value The corresponding macro document of the mensuration program of body metabolic system carries out computer statistics analysis, obtains every daily CH of test sheep4Row High-volume.
According to test sheep CH4Day discharge amount, calculate the CH of its per dry matter feed intake4Discharge amount (L/kg DMI), The CH of unit metabolic body size4Discharge amount (L/kg W0.75), the CH of per dry matter feed intake and metabolic body size4Discharge amount (L/kg W0.75 kg-1DMI) and unit can digest the CH of dry matter intake4Discharge amount (L/kg DDMI).
4.3 digestion and metabolism rates
The daily morning that is collected in of excrement sample and urine sample raises preceding carry out.When collecting excrement sample, by the corresponding receipts colostomy bag of every test sheep It removes and weighs weight, record the Fecal wastes on the day before it, after mixing by the excrement sample of every sheep, carried out by the 10% of Fecal wastes Sampling.When collecting urine sample, the H of 100mL 10% is first added before collecting into collection wooden pail for urine2SO4It is recorded with solid urinary nitrogen, after collection every Only the corresponding volume of urine of test sheep is sampled then with 4 layers of filtered through gauze by the 10% of every mutton chop urine volume.It will receive daily The excrement sample and urine sample of collection are stored in -20 DEG C of refrigerators.This process is carried out continuously 6 d, and all samples being collected into of every sheep are mixed It is spare that -20 DEG C of refrigerators are stored in after closing uniformly.After digest supersession test, the excrement sample of every sheep is placed in 65 DEG C of baking ovens and is dried It weighs after dry 48 h, 48 h that get damp again, obtains just moisture content, crushed 40 mesh mesh screens after and analysis sample is made, with back-up Analysis detection.
According to energy (GE), the dry matter of method the measurement feed and excrement of " forage analysis and determination of feeds quality technology " (DM), organic matter (OM), nitrogen (N), neutral detergent fiber (NDF), acid detergent fiber (ADF) content and urine in energy and Nitrogen content, and calculate corresponding digestibility and metabolic rate.
Total energy intake (GE)=diet dry substance feed intake × daily ration total energy content；
Methane can (CH4- E)=methane emission (L) × 0.714(g/L) × 10-3×55.65MJ/kg；
Total energy discharge rate in digestible energy (DE)=total energy intake (GE)-excrement, wherein total energy discharge rate=excrement discharge is total in excrement Total energy content in amount × excrement；
Total energy discharge rate-methane energy in total energy discharge rate-urine, total in excrement in metabolic energy (ME)=total energy intake (GE)-excrement Total energy content in total amount × excrement is discharged in energy output=excrement, total energy content in total energy discharge rate=urine discharge total amount × urine in urine；
Certain nutriment apparent digestibility (%)=(the nutriment discharge rate in the nutriment intake-excrement)/battalion Substance intake × 100% is supported, wherein the nutriment contains in the nutriment intake=diet dry substance feed intake × daily ration Amount；
Gross energy digestibility (DE/GE) (%)=(total energy discharge rate in total energy intake-excrement)/total energy intake × 100%；
Gross energy metabolic rate (ME/GE) (%)=(discharge total energy-CH in total energy-urine is discharged in intake total energy-excrement4Can)/take the photograph Enter total energy × 100%.
5. data processing
Test data application Excel carries out edit, then carries out single factor test with the ANOVA in statistical software SAS 9.2 Variance analysis, if carrying out Multiple range test with DunanShi method when significant difference, withP< 0.05 judgment criteria as significant difference.
6. result
Influence of the 6.1 methane adjusting control agents to discharge of methane: 2 are shown in Table
Influence of the 2 methane adjusting control agent of table to mutton sheep discharge of methane
Table 2 is as the result is shown: can be seen that from data result, B, C, D, E group have the discharge of methane of mutton sheep a degree of Inhibit, and most significant with inhibitory effect of the B group to discharge of methane.In mutton sheep discharge of methane total amount, B, C group do not drop than component A Low 10.5%, 0.8%；D, E group does not have advantage.It can digest in dry matter basis, B, C, D, E group mutton sheep methane emission point 17.5%, 9.66%, 9.62%, 7.67% is not reduced.
Influence of the 6.2 methane adjusting control agents to mutton sheep Nutrient Digestion And Metabolism: 3 are shown in Table
Influence of the 3 methane adjusting control agent of table to mutton sheep nutriment apparent digestibility
Table 3 is as the result is shown: compared with A group, dry matter, organic matter, neutral detergent fiber, the acidic cleaning of B, C, D, E group The apparent digestibility of fiber, nitrogen etc. is significantly increased, and D group is higher than other each groups, and B, C, E group difference is not significant.
The influence that 6.3 methane adjusting control agents are metabolized mutton sheep energy consumption: 4 are shown in Table
The influence that 4 methane adjusting control agent of table is metabolized mutton sheep energy consumption
Table 4 is as the result is shown: compared with A group, the methane of B group can and methane energy/eat total energy significantly reduce (P< 0.05), and D group then on the contrary, significantly increase methane can and methane energy/eat total energy (P< 0.05), the above indicator difference of C, E group is not significant (P> 0.05).
B, C, D, E group be significantly increased mutton sheep digestible energy and total energy digestibility (P< 0.05), wherein D group promotion amplitude Maximum, B, C, E group difference it is not significant (P> 0.05).B, C, D, E group can significantly improve the metabolic energy and total energy metabolic rate of mutton sheep (P< 0.05).
Brief summary:
(1) methane adjusting control agent provided by the invention can reduce the CH of mutton sheep4Discharge amount, B, C, D, E group mutton sheep can digest The CH of dry matter basis4Discharge amount (L/kg DDMI) reduces 17.5%, 9.66%, 9.62%, 7.67% respectively, wherein most with B group Good, D, E group are although lower, but daily discharge of methane total amount improves, and the discharge amount on the basis of dry matter intake is higher.Therefore most Good additive amount is advisable with B group；
(2) dry matter, organic matter, neutral detergent fiber and acidic cleaning can be improved in methane adjusting control agent provided by the invention The apparent digestibility of fiber, nitrogen improves digestible energy, metabolic energy, total energy digestibility and total energy metabolic rate.
Although above having used general explanation, specific embodiment and test, the present invention is made to retouch in detail It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art 's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed Range.

Claims (8)

1. a kind of ruminant methane emission reduction feed, which is characterized in that the feed contains the ingredient of following parts by weight: methane tune Control agent 5-10 parts, 6-20 parts of vitamin intermixture, 40-80 parts of minerals intermixture, 8.5-38 parts of feed vector；
Every part of methane adjusting control agent is composed of the following components in parts by weight: 8-10 parts of candida tropicalis, calcium chloride 15-20 Part, 20-35 parts of ammonium sulfate, 20-35 parts of lactose；
Every part of vitamin intermixture is to be composed of the following components in parts by weight: 1.2-4 parts of vitamin A, vitamin D 0.2- 0.5 part, 3-10 parts of vitamin E, 85-95 parts of vitamin mixing agent carrier, the vitamin mixing agent carrier are maize cob meal；
Every part of minerals intermixture is composed of the following components in parts by weight: 5-10 parts of ferrous sulfate monohydrate, cupric sulfate pentahydrate 0.8-2.0 parts, 5-10 parts of manganese sulfate monohydrate, 5-10 parts of monohydrate zinc sulphate, 0.8-2 parts of the cobalt chloride containing cobalt 1%, containing iodine 1% 2-4 parts of potassium iodide, 0.5-1 parts of the sodium selenite containing selenium 1%, 61-81 parts of minerals mixing agent carrier, the minerals intermixture Carrier is maize cob meal, corn protein powder or powdered rice hulls；
The feed vector is maize cob meal, corn protein powder, powdered rice hulls.
2. feed according to claim 1, which is characterized in that contain the ingredient of following parts by weight: methane in the feed 8-10 parts of adjusting control agent, 12-20 parts of vitamin intermixture, 40-54 parts of minerals intermixture, 18-38 parts of feed vector.
3. feed according to claim 2, which is characterized in that contain the ingredient of following parts by weight: methane in the feed 8 parts of adjusting control agent, 20 parts of vitamin intermixture, 54 parts of minerals intermixture, 18 parts of feed vector.
4. feed according to claim 1, which is characterized in that the methane adjusting control agent is by following parts by weight at grouping At: 10 parts of candida tropicalis, 20 parts of calcium chloride, 35 parts of ammonium sulfate, 35 parts of lactose.
5. feed according to claim 1, which is characterized in that every part of vitamin intermixture is by following parts by weight At being grouped as: 1.2-2.0 parts of vitamin A, 0.2-0.5 parts of vitamin D, 3.6-6 parts of vitamin E, vitamin mixing agent carrier 91-95 parts.
6. feed according to claim 5, which is characterized in that every part of vitamin intermixture by following parts by weight at It is grouped as: 1.2 parts of vitamin A, 0.2 part of vitamin D, 3.6 parts of vitamin E, 95 parts of vitamin mixing agent carrier.
7. feed according to claim 1, which is characterized in that every part of minerals intermixture by following parts by weight at It is grouped as: 7.6-10 parts of ferrous sulfate monohydrate, 1.2-2.0 parts of cupric sulfate pentahydrate, 7.3-10 parts of manganese sulfate monohydrate, monohydrate zinc sulphate 7.3-10 parts, 1.3-2 parts of the cobalt chloride containing cobalt 1%, 3.5-4 parts of the potassium iodide containing iodine 1%, the sodium selenite 0.8-1 containing selenium 1% Part, 61-71 parts of minerals mixing agent carrier.
8. feed according to claim 7, which is characterized in that every part of minerals intermixture is by following weight percent At being grouped as: 7.6 parts of ferrous sulfate monohydrate, 1.2 parts of cupric sulfate pentahydrate, 7.3 parts of manganese sulfate monohydrate, 7.3 parts of monohydrate zinc sulphate, 1.3 parts of the cobalt chloride containing cobalt 1%, 3.5 parts of the potassium iodide containing iodine 1%, 0.8 part of sodium selenite, minerals intermixture containing selenium 1% 71 parts of carrier.
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