CN105995061B - Application of the lactobacillus plantarum on preparation ruminant methane emission reduction feed - Google Patents
Application of the lactobacillus plantarum on preparation ruminant methane emission reduction feed Download PDFInfo
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- CN105995061B CN105995061B CN201610399742.5A CN201610399742A CN105995061B CN 105995061 B CN105995061 B CN 105995061B CN 201610399742 A CN201610399742 A CN 201610399742A CN 105995061 B CN105995061 B CN 105995061B
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- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 108
- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 57
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 57
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 54
- 241000282849 Ruminantia Species 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 230000009467 reduction Effects 0.000 title claims abstract description 7
- 241001494479 Pecora Species 0.000 claims description 45
- 239000006041 probiotic Substances 0.000 claims description 8
- 235000018291 probiotics Nutrition 0.000 claims description 8
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 7
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
- 235000013312 flour Nutrition 0.000 claims description 7
- 150000003839 salts Chemical class 0.000 claims description 7
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 6
- 241000186660 Lactobacillus Species 0.000 claims description 6
- 239000004615 ingredient Substances 0.000 claims description 6
- 229940039696 lactobacillus Drugs 0.000 claims description 6
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims description 4
- 235000019700 dicalcium phosphate Nutrition 0.000 claims description 4
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 3
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 3
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 3
- 239000004472 Lysine Substances 0.000 claims description 3
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 3
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 3
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 3
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 claims description 3
- 229930003316 Vitamin D Natural products 0.000 claims description 3
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims description 3
- 229930003427 Vitamin E Natural products 0.000 claims description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 3
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims description 3
- 239000010941 cobalt Substances 0.000 claims description 3
- 229910017052 cobalt Inorganic materials 0.000 claims description 3
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 239000010949 copper Substances 0.000 claims description 3
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- 229930182817 methionine Natural products 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
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- 229910052711 selenium Inorganic materials 0.000 claims description 3
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- 235000019165 vitamin E Nutrition 0.000 claims description 3
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- 239000011701 zinc Substances 0.000 claims description 3
- 229910052725 zinc Inorganic materials 0.000 claims description 3
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 2
- 238000004364 calculation method Methods 0.000 claims description 2
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 claims description 2
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 20
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- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 5
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- 206010028980 Neoplasm Diseases 0.000 description 4
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- 229910052739 hydrogen Inorganic materials 0.000 description 4
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- 241000194108 Bacillus licheniformis Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 241000194032 Enterococcus faecalis Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
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- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
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- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/22—Methane [CH4], e.g. from rice paddies
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention relates to application of the lactobacillus plantarum on preparation ruminant methane emission reduction feed.Lactobacillus plantarum be used in ruminant feed reduce cud Methane production be it is of the invention pioneering, in application, additive amount is low, can individually be mixed with water, also may be combined in feed and use, and addition is convenient.On the basis of reducing discharge of methane, nutritive digestibility still can be improved, be better than other methane inhibitors.Studies have shown that lactobacillus plantarum can reduce Methane Emission from Ruminants to a certain extent, lumen fermentation performance is improved, improves lumen fermentation type, and improve the capacity usage ratio of feed, there are the advantages such as safe, practical, effective.
Description
Technical field
The present invention relates to ruminants to cultivate field, and in particular to can reduce the probiotics of cattle and sheep methane emission.
Background technique
In ruminant production, because of methane (CH4) form loss energy account for feed total energy 2% ~ 15%, this is not only caused
The waste of feed resource, and to being harmful to atmospheric environment.Ruminant tumor gastric is a dynamic ecosystem, CH4's
Generation is a complicated process, is the inevitable product of carbohydrate fermentation in cud.However the generation of methane can add
Acute greenhouse effects reduce efficiency of feed utilization, need to regulate and control it using scientific and rational measure.
It is existing many about the research of cud methane regulation at present, the understanding of Methanogenesis is gradually deepened.Micro- life
State preparation is as novel green additive, the more collection of application to the Small side effects of animal and environment, in ruminant production
In growth promotion, anti-disease, improve in terms of, the report in terms of methane regulation is less.
Therefore, suitable micro-ecological additive and its scientific and rational additive capacity are found to reduce ruminant methane
Discharge amount have critically important research significance.
Existing methane inhibitor uses microorganism, organic compound, plant or plant extracts etc., in which: uses
Feed of the organic compound as methane inhibitor, it is possible to because change feed palatability and influence animal feed intake and
Growth；Plant and plant extracts, which are largely reported, confirms to have regulation methane discharge of ruminant positive effect, but
It is not conventional raw material or additive in ruminant feed, supply range and supply amount is influenced by the place of production, yield, still not
It can popularize on a large scale.In terms of microorganism, CN201310161752.1(Publication No. CN104137944A) disclose reproducibility
Acetogen, which has, inhibits the methane in stomach of ruminants to generate function Proteiniphilum acetatigenes SROD-1
(KCCM11219P) and its method is utilized.
Probiotics is defined as under microecology theoretical direction, and isolated beneficial microbe out of animal body is passed through
Active bacteria formulation containing only viable bacteria or comprising thallus and its metabolite made of special process, can improve the micro- life of animal gastrointestinal tract
The object ecological balance is beneficial to a kind of microbe additive of animal health and production performance performance.Probiotics answering in feed
With more and more extensive, molding preparation is also more, is suitble to large-scale production and application.The type of probiotics mainly has at present: lactic acid
Bacterium, bacillus, yeast and aspergillus class.Wherein lactic acid bacteria is one of the beneficial bacterium it is found that earliest, is widely used.Lactic acid
The substances such as organic acid, bacterium surface ingredient, the special enzyme system that bacterium is generated by fermentation, can inhibit spoilage organisms and pathogen, raising
Animal immunizing power, reduction cholesterol, improvement animal product quality etc., can stimulate tissue development, nutritional status, physiology to body
The generations such as function, cell infection, drug effect, toxic reaction, immune response, tumour generation, aging course and stress reaction are made
With.Lactic acid bacteria has many advantages, such as pollution-free, noresidue, does not generate drug resistance as feed addictive, and can improve feed wind
Taste promotes growth of animals or poultry, improves immunity, increases animal to the resistance of disease, has in current Animal husbandry production good
Development trend and application prospect.
The research of positive effect in relation to lactic acid bacteria in animal productiong focuses mostly in treatment diarrhea, reduces the death rate, increasing
Strong immunity of organisms improves premunition and production performance etc., in ruminant tumor gastric CH4The research of aspect does not appear in the newspapers yet
Road.
Lactobacillus plantarum (Lactobacillus plantarum) be lactic acid bacteria one kind, optimum growth temperature be 30 ~ 35
DEG C, anaerobism or amphimicrobian, strain be it is straight or curved rod-shaped, individually, sometimes in pairs or at chain, optimal pH 6. 5 or so belongs to
In homofermentative lactic bacteria.Applied in feed, it has been found that function have, immunological regulation, inhibit pathogenic bacteria, maintain enteron aisle in bacterium
Group's balance, promotes absorption of nutrient ingredients etc..
CN104719615A(application No. is 201510036493.9) be related to a kind of compound lactobacillus rumen regulation and control agent and its
Preparation method.The compound lactobacillus rumen regulation and control agent is by lactobacillus plantarum metabolin and the lactobacillus plantarum of inactivation, Lactococcus lactis
The enterococcus faecalis of the lactic acid bacteria of bacterium metabolin and inactivation, enterococcus faecalis metabolin and inactivation forms, the compound lactobacillus cud tune
Control agent can promote and safeguard microflora balance in cud, stablize ruminal pH value, improve the yield of cud volatile fatty acid,
Improve the content of mycoprotein.
It is write exactly in " influence of bacillus licheniformis and its compound bacteria to replacement cattle growth performance and Rumen Internal Environment " text,
With the increase at replacement cattle age, dominant bacteria type starts to reduce in rumen fluid, final to keep stablizing；Addition participates in the experiment probiotics also
Improve Rumen Cellulolytic Bacteria quantity (Fu Yunqin, Master's thesis, bacillus licheniformis and its compound bacteria to replacement cattle grow
The influence of performance and Rumen Internal Environment).According to methane generating process it is expected that cellulose-decomposing bacteria quantity improves, tumor can be improved
The yield of hydrogen in stomach, to promote the generation of methane.Meanwhile the study found that in animal rumens cellulose-decomposing bacteria quantity and first
Alkane bacterium number amount is positively correlated.To sum up, the raising of Rumen Cellulolytic Bacteria quantity may result in methane production raising, rather than drop
It is low.It is mentioned in " screening and identification of probiotics and its influence " to weanling pig, calf growth and alimentary canal microorganism text,
Near Watershed, Daxing County, Beijing City kind pig farm in soil isolated one plant can inhibit pathogen Escherichia coli and staphylococcus aureus
The lactic acid bacteria GF103 of growth, through 16s rRNA gene sequencing be accredited as lactobacillus plantarum (Lactobacillus plantarum), it is added to feeding in basal diet, the weight of piglet can be obviously increased, feed conversion rate is higher, piglet excrement
The quantity of middle Escherichia coli significantly reduces, the significantly whole height of serum IgM concentration, the results showed that, daily ration adds lactobacillus plantarum, can change
Kind weanling pig early stage growth performance (Chinese Academy of Agricultural Sciences, doctoral thesis, Dong Xiaoli, the screening and identification of probiotics and its
Influence to weanling pig, calf growth and alimentary canal microorganism).
By retrieval, lactic acid bacteria can be used for the increase of methane-generating pit methane production, and reduce the discharge of ruminant methane cud
The application aspect of amount has not been reported.
Summary of the invention
The object of the present invention is to provide application of the lactobacillus plantarum on preparation ruminant methane emission reduction feed.
In above-mentioned application:
The lactobacillus plantarum (Lactobacillus plantarum) it is any lactobacillus plantarum.
Preferably, the lactobacillus plantarum is lactobacillus plantarum GLP01(GenBank accession number EU626013.1).
In application, drinking after can dissolving plant lactobacillus powder with water for animal, or lactobacillus plantarum is mixed into feed
Middle use.
In the feed, lactobacillus plantarum 4 × 10 is added in every kilogram of feed dry matter9 CFU ~4×1011 CFU。
Preferably, lactobacillus plantarum 4 × 10 is added in every kilogram of feed dry matter9 CFU ~4×1010 CFU。
It is further preferred that adding lactobacillus plantarum 4 × 10 in every kilogram of feed dry matter10 CFU。
The feed is made of (dry matter basis) following parts by weight ingredient: 50 ~ 70 parts of sheep's hay, 17 ~ 29 parts of corn, and dregs of beans
10 ~ 19 parts, 1.0 ~ 2.0 parts of calcium monohydrogen phosphate, 0.3 ~ 0.5 part of mountain flour, 0.4 ~ 0.8 part of salt, 0.1 ~ 0.3 part of premixed feed.
Preferably, the feed is made of (dry matter basis) following parts by weight ingredient: 66.4 parts of sheep's hay, corn 19.1
Part, 12.2 parts of dregs of beans, 1.25 parts of calcium monohydrogen phosphate, 0.35 part of mountain flour, 0.5 part of salt, 0.2 part of premixed feed.
The commercially available purchase of premixed feed can provide (minimum guarantee for every kilogram of feed (ingredient in addition to sheep's hay)
Value): vitamin A 1.0 ~ 1.5 ten thousand IU, 2000 ~ 4000IU of vitamin D, 50 ~ 90g of vitamin E, vitamin B1 0.1~
0.5mg, vitamin B20.2 ~ 0.8mg, 0.1 ~ 0.5mg of niacin, 18 ~ 25g of lysine, 10 ~ 15g of methionine, tryptophan 2.0 ~
3.5g, 50 ~ 120mg of iron, 10 ~ 15mg of copper, 90 ~ 130mg of zinc, 100 ~ 140mg of manganese, 0.1 ~ 0.5mg of selenium, 1.0 ~ 1.5mg of iodine, cobalt
The micro constitutents such as 0.2 ~ 0.8mg.
The new application of lactobacillus plantarum provided by the invention has the advantage that
1. lactobacillus plantarum be used in the Methane production reduced in ruminant feed be it is of the invention pioneering, applying
When additive amount it is low, drunk after can individually being mixed with water, also may be combined in feed and use, addition is convenient.Reducing methane
On the basis of discharge, nutritive digestibility still can be improved, be better than other methane inhibitors.
2. inventor abandons the defect of in vitro test, the method for surveying methane emission by respiratory metabolism test method,
Effective methane inhibitor and its adding proportion are filtered out, can be directly applied to without conversion in ruminant premixed feed,
It is horizontal to reduce ruminant aquaculture discharge of methane, improves production economy benefit.The addition ratio obtained is tested using in vitro culture
, it needs to be converted according to factors such as feed intake, cud volumes in production, practical application effect needs further to examine.
3. by actual measurement 24 hours carbon dioxide of mutton sheep, methane emission, in the different additive amount of lactobacillus plantarum
In filter out the minimum group of methane emission, so that it is determined that its suitable proportion in ruminant feed.Studies have shown that plant
Lactobacillus can reduce Methane Emission from Ruminants to a certain extent, improve lumen fermentation performance, improve lumen fermentation type,
And the capacity usage ratio of feed is improved, there are the advantages such as safe, practical, effective.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
The present embodiment is only with lactobacillus plantarum GLP01(GenBank accession number: EU626013.1) (see " the ensiling source Hong Meizhu
The research of lactic acid bacteria culture process and ferment effect ", the Chinese Academy of Agricultural Sciences, 2011, Master's thesis) for the tool of invention
Hold in vivo and elaborate, but does not limit the selection of strain source of the present invention, lactobacillus plantarum GLP01 viable bacteria unit 2 × 1010
CFU/g is stored by Institute of Feeds,China Academy of Agriculture Sciences；
Premixed feed is produced by Beijing Precise Animal Nutrient Research Center, be every kilogram of feed (in addition to sheep's hay feed at
Point) (minimum guarantee value) is provided: 1.2 ten thousand IU of vitamin A, vitamin D 3000IU, vitamin E 70g, vitamin B1
0.3mg, vitamin B20.5mg, niacin 0.3mg；Lysine 22g, methionine 12g, tryptophan 2.8g；Iron 90mg, copper 12mg,
The micro constitutents such as zinc 110mg, manganese 120mg, selenium 0.3mg, iodine 1.3mg, cobalt 0.5mg.
Other feed addictives and feedstuff are all obtained from feed market purchase.
Embodiment 1: lactobacillus plantarum reduces the application of discharge of methane in ruminant feed
The dosage of lactobacillus plantarum is that 4 × 10 are added in every kilogram of feed dry matter9CFU lactobacillus plantarum.
Feed is made of (dry matter basis) following weight percent: sheep's hay 70%, corn 17%, dregs of beans 10.98%, phosphoric acid
Hydrogen calcium 1.1%, mountain flour 0.3%, salt 0.45%, premixed feed 0.17%.
Embodiment 2: lactobacillus plantarum reduces the application of discharge of methane in ruminant feed
The dosage of lactobacillus plantarum is that 4 × 10 are added in every kilogram of feed dry matter10 CFU。
Feed is made of (dry matter basis) following weight percent: sheep's hay 66.4%, corn 19.1%, dregs of beans 12.2%, phosphorus
Sour hydrogen calcium 1.25%, mountain flour 0.35%, salt 0.5%, premixed feed 0.2%.
Embodiment 3: lactobacillus plantarum reduces the application of discharge of methane in ruminant feed
The dosage of lactobacillus plantarum is that 4 × 10 are added in every kilogram of feed dry matter11 CFU。
Feed is made of (dry matter basis) following weight percent: sheep's hay 50%, corn 28.4%, dregs of beans 18.2%, phosphoric acid
Hydrogen calcium 1.9%, mountain flour 0.5%, salt 0.7%, premixed feed 0.3%.
Experimental example 1: lactobacillus plantarum reduces the application effect verifying of discharge of methane in ruminant feed
1. test material and equipment
Lactobacillus plantarum GLP01(GenBank accession number EU626013.1), viable bacteria unit 2 × 1010CFU/g, by China
The storage of feed research institute, Academy of Agricultural Sciences, Beijing Hua Nong bioengineering Co., Ltd expand numerous and produce.
Methane surveys device: Open path gas metabolic system, LGR gas analyzer are purchased from Sable company, the U.S..It is a whole set of
Experimental rig is made of the measurement device of Sable system, closed gas respiratory chamber and matched computer software.It once can be same
When measure 6 sheep whole day breathing gas and methane emission.
2. experimental animal and design
Experimental animal: it is (51.75 ± 1.48) kg that 24 weight are chosen in test, and the age is close, the good Du Bomian of body condition
Sheep (♂) × Small-fat-tail sheep (♀) first familiar generation adult wether.
Experimental design: test uses single factor test completely random experimental design, and 24 sheep are divided into 4 processing groups, i.e., 1
Control group and 3 test groups, every group of 6 sheep, every sheep is 1 repetition, and (formula is shown in embodiment 2 to control group fed Basic drawing
The feed of offer), 3 test groups add lactobacillus plantarum on the basis of Basic drawing, in which:
Low dose group: pitch-based sphere is that 4 × 10 are added in every kilogram of feed dry matter9CFU；
Middle dose group: pitch-based sphere is that 4 × 10 are added in every kilogram of feed dry matter10CFU；
High dose group: pitch-based sphere is that 4 × 10 are added in every kilogram of feed dry matter11 CFU。
31 d of experimental period, wherein 18 d of preliminary trial period, positive to try 13 d of phase.
3. testing daily ration: Basic drawing is made of fine fodder and sheep's hay, sees the feed that embodiment 2 provides, and trophic level is shown in
Table 1.
1 Basic drawing trophic level of table (%, dry matter basis)
4. testing index and method
4.1 test sheep weight
When carrying out gaseous metabolism test, weight survey is carried out to it respectively when testing sheep and entering and leaving gaseous metabolism room
It is fixed, calculation basis of the average weight measured twice as test sheep metabolic body size.
Metabolic body size (W0.75)=average weight0.75。
4.2 CH4Discharge amount
CH4Discharge amount is measured using Open path gas metabolic system (Sable, USA), and system connects 6 breathings and surveys
Hot tank can measure the CH of 6 sheep simultaneously4Discharge amount.It is furnished with hopper and sink in each breath heat measurement case, test sheep is being tested
Period can be freely eaten and drink water.After test sheep enters breath heat measurement case, 24 h are first adapted to, then measure the CH of 48 h4Discharge
Amount.Measuring the system circulation primary time is 60 min.
When starting measurement, system measures CH in experimental enviroment first4Content, minute be 2 min, then by environment
It is replaced into breath heat measurement case, time swap is 1 min, then the CH of 6 breath heat measurement casees of sequentially determining4Discharge amount, each
Breath heat measurement case minute is 9 min, and then system is replaced from breath heat measurement case to environment, and time swap is 1 min, finally
The CH in experimental enviroment is measured again4Content, minute are 2 min.The above are the completions of Sable Open path gas metabolic system
The measurement process of one cycle, the CH of 48 h of METHOD FOR CONTINUOUS DETERMINATION is recycled with this4Discharge amount.
In calculating process, with the CH measured in experimental enviroment twice4The average value of content passes through system as substrate value
The corresponding macro document of the mensuration program of Sable gaseous metabolism system carries out computer statistics analysis, and it is daily to obtain every test sheep
CH4Discharge amount.
According to test sheep CH4Day discharge amount, calculate the CH of its per dry matter feed intake4Discharge amount (L/kg DMI),
The CH of unit metabolic body size4Discharge amount (L/kg W0.75), the CH of per dry matter feed intake and metabolic body size4Discharge amount (L/kg
W0.75 kg-1DMI) and unit can digest the CH of dry matter intake4Discharge amount (L/kg DDMI).
4.3 digestion and metabolism rates
The daily morning that is collected in of test sheep dung sample and urine sample raises preceding carry out.It is when collecting excrement sample, every test sheep is corresponding
Receipts colostomy bag, which is removed, weighs weight, the Fecal wastes on the day before it is recorded, after mixing by the excrement sample of every sheep, by Fecal wastes
10% is sampled.When collecting urine sample, the H of 100mL 10% is first added before collecting into collection wooden pail for urine2SO4With solid urinary nitrogen, after collection
The corresponding volume of urine of every test sheep is recorded, then with 4 layers of filtered through gauze, is sampled by the 10% of every mutton chop urine volume.
The excrement sample and urine sample collected daily are stored in -20 DEG C of refrigerators.This process is carried out continuously 6 d, and by every sheep institute
There is the sample being collected into be stored in -20 DEG C of refrigerators after mixing spare.After digest supersession test, by the excrement sample of every sheep
65 DEG C of 48 h of drying in oven are placed in, are weighed after 48 h that get damp again, just moisture content is obtained, crushed 40 mesh mesh screens after and be made
Sample is analyzed, in case analysis detection.
According to energy (GE), the dry matter of method the measurement feed and excrement of " forage analysis and determination of feeds quality technology "
(DM), organic matter (OM), nitrogen (N), neutral detergent fiber (NDF), acid detergent fiber (ADF) content and urine in energy and
Nitrogen content, and calculate corresponding digestibility and metabolic rate.
Total energy intake (GE)=diet dry substance feed intake × daily ration total energy content；
Methane can (CH4- E)=methane emission (L) × 0.714(g/L) × 10-3×55.65MJ/kg；
Total energy discharge rate in digestible energy (DE)=total energy intake (GE)-excrement, wherein total energy discharge rate=excrement discharge is total in excrement
Total energy content in amount × excrement；
Total energy discharge rate-methane energy in total energy discharge rate-urine, total in excrement in metabolic energy (ME)=total energy intake (GE)-excrement
Total energy content in total amount × excrement is discharged in energy output=excrement, total energy content in total energy discharge rate=urine discharge total amount × urine in urine；
Certain nutriment apparent digestibility (%)=(the nutriment discharge rate in the nutriment intake-excrement)/battalion
Substance intake × 100% is supported, wherein the nutriment contains in the nutriment intake=diet dry substance feed intake × daily ration
Amount；
Gross energy digestibility (DE/GE) (%)=(total energy discharge rate in total energy intake-excrement)/total energy intake × 100%.
Gross energy metabolic rate (ME/GE) (%)=(discharge total energy-CH in total energy-urine is discharged in intake total energy-excrement4Can)/take the photograph
Enter total energy × 100%.
5. data processing
Test data application Excel carries out edit, then carries out single factor test with the ANOVA in statistical software SAS 9.2
Variance analysis, if carrying out Multiple range test with DunanShi method when significant difference, withP< 0.05 judgment criteria as significant difference.
6. result
Influence of 6.1 lactobacillus plantarums to discharge of methane: 2 are shown in Table
Influence of 2 lactobacillus plantarum of table to mutton sheep discharge of methane
Note: colleague's data shoulder mark same letter or without letter indicate difference it is not significant (P> 0.05), shoulder mark difference small letter
Matrix show significant difference (P< 0.05).Following table is same.
Table 2 is as the result is shown: after being added to lactobacillus plantarum in mutton sheep diet, compared with the control group, middle dose group is significant
Reduce CH4Day discharge amount (L/d) (P< 0.05), the basis dry matter intake (DMI) methane emission (L/kgDMI) (P
< 0.05), the methane emission (L/kgW on metabolic body size basis0.75) (P< 0.05) and dry matter intake metabolic body size basis
Methane emission (L/kgW0.75﹒ kg-1DMI) (P< 0.05).
Under identical dry matter intake, compared with the control group, low and middle dose group, which significantly reduces, can digest dry matter
Basis methane emission (L/kg DDMI) (P< 0.05), this index of high dose group then difference it is not significant (P> 0.05).
As can be seen from the test results, lactobacillus plantarum has a degree of inhibiting effect to the discharge of mutton sheep methane,
And with middle dose group, i.e., when the pitch-based sphere of lactobacillus plantarum is 4 × 10 in every kilogram of feed dry matter10Discharge of methane when CFU
The inhibitory effect of amount is most significant.
Influence of 6.2 lactobacillus plantarums to mutton sheep Nutrient Digestion And Metabolism: 3 are shown in Table
Influence of 3 lactobacillus plantarum of table to mutton sheep nutriment apparent digestibility
Table 3 is the results show that compared with the control group, dry matter, organic matter, the neutral detergent fiber of low, medium and high dosage group
It is significantly increased with the apparent digestibility of nitrogen etc..
The influence that 6.3 lactobacillus plantarums are metabolized mutton sheep energy consumption: 4 are shown in Table
The influence that 4 lactobacillus plantarum of table is metabolized mutton sheep energy consumption
Table 4 is as the result is shown: compared with the control group, the methane of middle dose group can and methane energy/eat total energy ratio it is aobvious
Work reduction (P< 0.05), the corresponding index of high dose group significantly increase (P< 0.05), then difference is not or not the corresponding index of low dose group
Significantly (P> 0.05).
3 lactobacillus plantarum addition groups improve mutton sheep digestible energy and total energy digestibility (P< 0.05), but difference adds
Add difference between group (P> 0.05) not significant.The metabolic energy and total energy metabolic rate for neutralizing high dose group are all remarkably higher than low dose group
With control group (P< 0.05), but between two groups difference it is not significant (P> 0.05), the corresponding index of low dose group is significantly higher than control
Group (P< 0.05).
Feed addictive of the lactobacillus plantarum as mutton sheep, can be improved the energy utilization efficiency of feed.
Brief summary:
Addition lactobacillus plantarum can reduce the methane emission of mutton sheep, improve the digestion of energy and part nutriment
Rate.When pitch-based sphere is 4 × 10 in every kilogram of feed dry matter9CFU and 4 × 1010When CFU, reducing mutton sheep can digest
The CH of dry matter basis4Discharge amount, the range of decrease are respectively 5.84% and 15.28%.When pitch-based sphere is 4 in every kilogram of feed dry matter
×1010When CFU, CH is reduced4Day discharge amount, when pitch-based sphere be every kilogram of feed dry matter in 4 × 1011When CFU, rise
High CH4Day discharge amount.It is every kilogram of feed dry matter that lactobacillus plantarum, which inhibits the best pitch-based sphere of mutton sheep discharge of methane,
In 4 × 1010 CFU。
Although above having used general explanation, specific embodiment and test, the present invention is made to retouch in detail
It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Range.
Claims (3)
1. application of the lactobacillus plantarum on preparation ruminant methane emission reduction feed, the lactobacillus plantarum
(Lactobacillus plantarum) is lactobacillus plantarum GLP01, the GenBank accession number of the lactobacillus plantarum GLP01
EU626013.1 is used in application, can mix lactobacillus plantarum in feed, in the feed, in every kilogram of feed dry matter
Probiotics only add lactobacillus plantarum, and dosage is 4 × 109CFU~4 × 1010CFU, the feed is by below in dry matter
Parts by weight ingredient composition in basic calculation: 50~70 parts of sheep's hay, 17~29 parts of corn, 10~19 parts of dregs of beans, calcium monohydrogen phosphate 1.0
~2.0 parts, 0.3~0.5 part of mountain flour, 0.4~0.8 part of salt, 0.1~0.3 part of premixed feed, the premixed feed is every
The nutrition of kilogram of feed contains following component: vitamin A 1.0~1.5 ten thousand IU, 2000~4000IU of vitamin D, vitamin E
50~90g, vitamin B10.1~0.5mg, vitamin B20.2~0.8mg, 0.1~0.5mg of niacin, 18~25g of lysine,
10~15g of methionine, 2.0~3.5g of tryptophan, 50~120mg of iron, 10~15mg of copper, 90~130mg of zinc, manganese 100~
140mg, 0.1~0.5mg of selenium, 1.0~1.5mg of iodine, 0.2~0.8mg of cobalt, the ruminant are sheep.
2. application according to claim 1, which is characterized in that in the feed, add and plant in every kilogram of feed dry matter
Object lactobacillus 4 × 1010CFU。
3. application according to claim 1, which is characterized in that the feed is by the weight below in dry matter basis calculating
Amount part is at being grouped as: 66.4 parts of sheep's hay, 19.1 parts of corn, and 12.2 parts of dregs of beans, 1.25 parts of calcium monohydrogen phosphate, 0.35 part of mountain flour, salt
0.5 part, 0.2 part of premixed feed.
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