Summary of the invention
The present invention is directed to the deficiency of prior art, provide a kind of and fatten sheep with rich selenium TRM prescription, and the production method of this feed, this feed of feeding grows up to fattens high more than 1.4 times than the normal diet of feeding of Se content in the sheep muscle.
Of the present inventionly fatten sheep and form by the raw material of following mass parts: nanometer selenium 0.4-0.6 part, nanometer copper 0.16-0.25 part, zinc methionine 0.12-0.20 part, methionine iron 0.20-0.30 part, manganese methionine 0.12-0.20 part, V with rich selenium TRM
APowder 0.032-0.06 part, V
DPowder 0.02-0.04 part, V
EPowder 0.06-0.08 part; Land plaster 0.4-0.5 part; Cellulase 0.08-0.125 part; Active dry yeast bacterium (20,000,000,000/g) 0.04-0.05 parts; Zeolite powder 0.4-0.5 part; Powdered rice hulls 1.52-2.33 part; Corn 216-300 part; Wheat bran 24-60 part; DDGS (maize alcohol stillage albumen) 36-60 part; Dregs of beans 20-28 part; Palm kernel meal 0-48 part; Sunflower cake 0-44 part; Rice bran 0-25 part; Oil cake of flax seed 0-50 part; Salt 2.4-3.0 part; Calcium monohydrogen phosphate 1.6-2.0 part; Corn stalk powder 150-220 part; Maize storage 180-300 part; Hay 80-170 part.
Of the present invention fatten sheep with rich selenium TRM branch fatten early stage rich selenium TRM with fatten rich selenium TRM of later stage; Wherein, fatten the early stage rich selenium TRM form by the raw material of following mass parts: nanometer selenium 0.4-0.48 part, nanometer copper 0.16-0.20 part, zinc methionine 0.12-0.16 part, methionine iron 0.20-0.24 part, manganese methionine 0.12-0.16 part, KI 0.04-0.06 part, cobalt chloride 0.06-0.08 part, V
APowder 0.032-0.048 part, V
DPowder 0.02-0.032 part, V
EPowder 0.06-0.08 part, 0.4 part of land plaster, cellulase 0.08-0.10 part, active dry yeast bacterium (20,000,000,000/g) 0.04 parts, 0.4 part of zeolite powder, powdered rice hulls 1.52-1.87 part; Corn 216-224 part, wheat bran 24-32 part, palm kernel meal 40-48 part, dregs of beans 24-28 part, sunflower cake 40-44 part, DDGS 36-40 part, 2.4 parts of salt, 1.6 parts of calcium monohydrogen phosphates; Corn stalk powder 200-220 part, maize storage 260-300 part and hay 80-120 part.
Fattening rich selenium TRM of later stage is made up of the raw material of following mass parts: nanometer selenium 0.5-0.6 part, nanometer copper 0.2-0.25 part, zinc methionine 0.15-0.20 part, methionine iron 0.25-0.30 part, manganese methionine 0.15-0.20 part, 0.05 ~ 0.075 part of KI, 0.075 ~ 0.10 part of cobalt chloride, V
APowder 0.040-0.06 part, V
DPowder 0.025-0.04 part, V
EPowder 0.075-0.08 part, 0.5 part of land plaster, cellulase 0.10-0.125 part, active dry yeast bacterium (20,000,000,000/g) 0.05 parts, 0.50 part of zeolite powder, powdered rice hulls 1.90-2.335 part; Corn 290-300 part, wheat bran 50-60 part, rice bran 20-25 part, dregs of beans 20-25 part, DDGS 55-60 part, oil cake of flax seed 40-50 part, 3.0 parts of salt, 2.0 parts of calcium monohydrogen phosphates; Corn stalk powder 150-160 part, maize storage 180-200 part and hay 140-170 part.
Described fattening is meant that fattening sheep was born back 90-130 days early stage.
Describedly fatten the later stage and be meant and fattened sheep birth back 131-180 days.
Described maize storage is that compacting, fermentation are processed with the blue or green cornstalk chopping behind the results seed, urea and the 3-5% wheat bran of interpolation 1%-3%.
It is following with the production stage of rich selenium TRM to fatten sheep:
(1) according to fattening rich selenium TRM prescription in early stage, take by weighing nanometer selenium, nanometer copper, zinc methionine once more mixing process, methionine iron, manganese methionine, V
APowder, V
DPowder, V
EPowder, land plaster, cellulase, the dried bacterium of active dry yeasr mix for the first time, and then claim that zeolite powder mixes for the second time; Get the powdered rice hulls of total amount 40%-60% again, mix for the third time, get residual rice hull powder mixing once more at last again; Be and fatten premix in early stage, mark is preserved subsequent use;
(2) according to fattening rich selenium TRM prescription in early stage; Take by weighing corn, wheat bran, palm kernel meal, dregs of beans, sunflower cake, maize alcohol stillage albumen, salt, calcium monohydrogen phosphate; Fatten premix in early stage with (1) preparation whole and mix, process and fatten the mixed concentrate in early stage, it is subsequent use that mark is preserved;
(3) be ready to the storage of corn stalk powder, maize, hay according to fattening rich selenium TRM prescription in early stage, taking by weighing again, fatten the early stage mixed concentrate with (2) preparation whole and mix, process and fatten rich selenium TRM in early stage;
(4) according to fattening later stage rich selenium TRM prescription, take by weighing nanometer selenium, nanometer copper, zinc methionine once more mixing process, methionine iron, manganese methionine, V
APowder, V
DPowder, V
EPowder, land plaster, cellulase, the dried bacterium of active dry yeasr mix for the first time, and then claim that zeolite powder mixes for the second time; Get the powdered rice hulls of total amount 40%-60% again, mix for the third time, get residual rice hull powder mixing once more at last again; Be and fatten later stage premix, mark is preserved subsequent use;
(5) according to fattening later stage rich selenium TRM prescription; Take by weighing corn, wheat bran, rice bran, dregs of beans, maize alcohol stillage albumen, oil cake of flax seed, salt, calcium monohydrogen phosphate; Fatten later stage premix and mix with the whole of (4) preparation, be and fatten the later stage mixed concentrate, mark is preserved subsequent use;
(6) according to fattening later stage rich selenium TRM prescription, take by weighing corn stalk powder, maize storage and hay again, the whole later stage mixed concentrates of fattening that prepare with (5) mix, and are to fatten rich selenium TRM of later stage.
The comparative test that rich selenium TRM of the present invention and normal diet are fed sheep
1, different selenium are originated and mutton are respectively organized the influence of Se content
(1) experimental animal: physically fit, select average weight 3 monthly ages of 20 ± 1.5 kg, 40 of public lambs, be divided into 4 groups at random, 10 every group.Experimental period is 105 days, 15 days preliminary trial periods of branch and just trying 90 days phases.Carry out routine immunization between preliminary trial period, get into and just try the phase.Experimental period, only finish to butcher sheep, gets various tissue samples, and freezing preservation is subsequent use.
(2) test daily ration: prepare with reference to U.S. NRC (version in 2007) sheep feeding standard.In TRM, add the selenium of 3 kinds of forms respectively: sodium selenite, selenium methionine and nanometer selenium; The interpolation level is that every kilogram of daily ration contains 0.3 milligram selenium; Control group does not add the selenium of any form, and premix is carrier with zeolite powder and powdered rice hulls, adopts and amplifies the mode of averaging preparation step by step.
(3) measuring selenium concentration: measure with atomic fluorescence method.Accurately take by weighing the 1g tissue sample in triangular flask with electronic balance; Add the 5ml red fuming nitric acid (RFNA), cover the cold digestion of little funnel and spend the night, add the 1ml hydrogen peroxide solution next day; Place 90 ℃ of heating 1h on the electric hot plate; Transfer to 130 ℃ again and be heated to solution and become limpid colourlessly, move to after the cooling in the 50ml volumetric flask, be settled to and go up machine (AFS-230E two pass atomic fluorescence spectrophotometer) behind the scale and detect.Result of the test is seen table 1.
The influence of Se content is respectively organized in the different selenium of table 1 source to mutton
Annotate: * * representes difference extremely significantly (p<0.01).
Result of the test shows that different selenium source respectively organizes the influence of Se content very big to mutton.Sodium selenite, three kinds of different selenium of selenium methionine and nanometer selenium utmost point of originating has all improved mutton significantly and has respectively organized Se content.In three kinds of different selenium sources, the nanometer selenium utmost point again is superior to sodium selenite and selenium methionine significantly.Therefore, Se-enriched feedstuff of the present invention will adopt nanometer selenium to originate as selenium.
2, the nanometer selenium of variable concentrations is respectively organized the influence of Se content to mutton
(1) experimental animal: physically fit, select average weight 3 monthly ages of 20 ± 1.5 kg, 40 of public lambs, be divided into 4 groups at random, 10 every group.Experimental period is 105 days, 15 days preliminary trial periods of branch and just trying 90 days phases.Carry out routine immunization between preliminary trial period, get into and just try the phase.Experimental period, only finish to butcher sheep, gets various tissue samples, and freezing preservation is subsequent use.
(2) test daily ration: prepare with reference to U.S. NRC (version in 2007) sheep feeding standard.In TRM, add 4 selenium levels respectively: 0,0.3,1.0 and 3.0 mg/kg daily rations, selenium source is chosen as nanometer selenium, and premix is carrier with zeolite powder and powdered rice hulls, adopts and amplifies the mode of averaging preparation step by step.
(3) measuring selenium concentration: in same 1 (3).Result of the test is seen table 2.
The nanometer selenium of table 2 variable concentrations is respectively organized the influence of Se content to mutton
Annotate: * * representes difference extremely significantly (p<0.01).
Visible from table 2; The nanometer selenium of variable concentrations respectively organizes the influence of Se content also very big to mutton; In the experimental concentration scope, when selenium adds concentration when being the nanometer selenium of 1.0mg/kg, Se content of each tissue of mutton all utmost point is significantly higher than low Se content during with higher concentration.So Se-enriched feedstuff of the present invention will be with 1.0mg/kg and certain interval up and down nanometer selenium concentration thereof as nanometer selenium addition in the feed.
3, feed of the present invention and the normal diet sheep of feeding is respectively organized the influence of Se content to mutton
(1) experimental animal: physically fit, select average weight 3 monthly ages of 20 ± 1.5 kg, 100 of public lambs, be divided into 2 groups at random, 50 every group.Experimental period is 105 days, 15 days preliminary trial periods of branch and just trying 90 days phases.Carry out routine immunization between preliminary trial period, get into and just try the phase.Get 5 at random and butcher for every group when experimental period finishes, get various tissue samples, freezing preservation is subsequent use.
(2) test daily ration: feed of the present invention and normal diet.
(3) measuring selenium concentration: in same 1 (3).Result of the test is seen table 3.
Table 3 feed of the present invention and the normal diet sheep of feeding is respectively organized the influence of Se content to mutton
Annotate: * * representes difference extremely significantly (p<0.01).
Can know by table 3 result of the test, feed with feed of the present invention and fatten sheep and significantly improved Se content in the sheep product than the normal diet utmost point.Through relatively, with feed of the present invention feed fatten sheep than normal diet Se content in musculature improved 140.58%, Se content has improved 232.66% in the liver organization, Se content has improved 81.39% in the cardiac muscular tissue, Se content has improved 128.6% in the renal tissue, the testis tissue Se content improved 241.67%.
4, feed of the present invention and the normal diet influence of sheep of feeding to feed conversion rate and fattening effect
(1) experimental animal: physically fit, select average weight 3 monthly ages of 20 ± 1.5 kg, 20 of public lambs, be divided into 2 groups at random, 10 every group.Experimental period is 105 days, 15 days preliminary trial periods of branch and just trying 90 days phases.Claim its daily ration scale of feeding, surplus material amount every day, calculate feed intake in experimental period.Weighed twice continuous two day morning on an empty stomach when fattening period began and finishes, and gets its mean value.
(2) test daily ration: feed of the present invention and normal diet.
(3) feed conversion rate: forage volume * 100% that the live-weight ÷ of feed conversion rate (%)=increase consumes.
Result of the test is seen table 4.
The influence of sheep of feeding of table 4 feed of the present invention and normal diet to feed conversion rate and fattening effect
The feed group |
Initial average weight (kg) |
End of term average weight (kg) |
Average daily gain (g) |
Feed conversion rate % |
Normal diet |
20.25 |
38.33 |
200.88 |
26.76 |
Feed of the present invention |
20.17 |
42.78 |
251.19 |
31.58 |
Increase % |
-- |
11.61 |
25.0 |
18.0 |
Test shows that average daily gain had increased by 25% when feed ratio normal diet of the present invention was fed sheep, and when fattening 180 days, average weight has increased by 11.61%; Feed conversion rate has improved 18.0%.
Beneficial effect of the present invention
(1) rich selenium TRM of the present invention has selected that toxicity is low for use, good absorbing effect, nanometer selenium that biologically active is high originate as selenium, and the consumption of nanometer selenium is through the concentration gradient experiment sieving nanometer selenium dosage of muscle Se content when the highest that comes out.Feed with feed of the present invention and to fatten the sheep utmost point and significantly improved each tissue selenium content of sheep product, wherein the musculature Se content has improved 140.58%.
(2) rich selenium TRM of the present invention is according to the sheep feed that the nutritional requirements of different phase is mixed with that grows; Use the feed of different formulations at the different growth phases of fattening sheep; Because various nutritional labeling collocation have rationally been added profitable probliotics and cellulase in addition in the feed, help to regulate and fatten the sheep rumen microflora; Cellulase can promote cellulosic digestion in the daily ration; Therefore, make the feed conversion rate of fattening sheep high, grow fast, respectively with 18% and 25% of the normal diet group of feeding.
(3) feed of the present invention can significantly improve Se content in the sheep product; And the growth and the health of fattening sheep there is facilitation; The mutton of producing is the safe selenium source that human body is mended selenium, and the functional mutton of producing rich selenium can increase raiser's income, and comprehensive social benefit is huge.
Embodiment 1
Prepare 1000 kilograms and fatten early stage rich selenium TRM and 1000 kilograms and fatten rich selenium TRM of later stage, method is following:
Fatten the preparation of rich selenium TRM in early stage:
(1) premix preparation in early stage: take by weighing 0.4 kilogram of nanometer selenium, 0.2 kilogram of nanometer copper, 0.12 kilogram of zinc methionine, 0.24 kilogram of methionine iron, 0.12 kilogram of manganese methionine, 0.06 kilogram of KI, 0.06 kilogram of cobalt chloride, V
A0.048 kilogram in powder, V
D0.02 kilogram in powder, V
E(0.4 kilogram of 20,000,000,000/g) 0.04 kilogram, land plaster mix, and then claim 0.4 kilogram of zeolite powder to mix for the second time for the first time for 0.08 kilogram in powder, 0.08 kilogram of cellulase, active dry yeast bacterium; Take by weighing 0.81 kilogram of powdered rice hulls again, mix for the third time; Take by weighing 1.0 kilograms of powdered rice hulls at last again, mixing is premix once more, and mark is preserved subsequent use;
(2) fine fodder compound preparation in early stage: take by weighing 216 kilograms of corns, 32 kilograms in wheat bran, 40 kilograms of palm kernel meals, 28 kilograms of dregs of beans, 40 kilograms of sunflower cakes, 40 kilograms of DDGS (maize alcohol stillage albumen), 2.4 kilograms of salt, 1.6 kilograms of calcium monohydrogen phosphates; Mix with whole premixes of (1) preparation; Be and fatten the mixed concentrate in early stage, mark is preserved subsequent use;
(3) take by weighing and be ready to 300 kilograms of 200 kilograms of corn stalk powders, maize storages, 80 kilograms in hay, mix, be 1000 kilograms and fatten rich selenium TRM in early stage with the mixed concentrate in whole early stage of (2) preparation.
Fatten the preparation of rich selenium TRM of later stage:
(1) later stage premix preparation: take by weighing 0.5 kilogram of nanometer selenium, 0.25 kilogram of nanometer copper, 0.15 kilogram of zinc methionine, 0.3 kilogram of methionine iron, 0.15 kilogram of manganese methionine, 0.075 kilogram of KI, 0.075 kilogram of cobalt chloride, V
A0.06 kilogram in powder, V
D0.025 kilogram in powder, V
E(0.5 kilogram of 20,000,000,000/g) 0.05 kilogram, land plaster mix first for 0.08 kilogram in powder, 0.10 kilogram of cellulase, active dry yeast bacterium; 0.5 kilogram of zeolite powder again behind the mixing mixes for the second time; Take by weighing 1.0 kilograms of powdered rice hulls again, once more mixing; Take by weighing 1.335 kilograms of powdered rice hulls at last, mixing is premix once more, and mark is preserved subsequent use;
(2) fatten later stage fine fodder compound preparation: take by weighing 300 kilograms of corns, 50 kilograms in wheat bran, 25 kilograms in rice bran, 20 kilograms of dregs of beans, 60 kilograms of DDGS, 40 kilograms of oil cake of flax seed, 3 kilograms of salt, 2 kilograms of calcium monohydrogen phosphates; Whole premixes of preparing with (1) mix; Be and fatten the later stage mixed concentrate, mark is preserved subsequent use;
(3) fatten later stage TRM preparation: claim 150 kilograms of corn stalk powders, 200 kilograms of maize storages, 140 kilograms in hay, the fatten later stage mixed concentrates whole with (2) mix, and are 1000 kilograms and fatten rich selenium TRM of later stage.
Embodiment 2
Prepare 1000 kilograms and fatten early stage rich selenium TRM and 1000 kilograms and fatten rich selenium TRM of later stage, method is following:
Fatten the preparation of rich selenium TRM in early stage:
(1) premix preparation in early stage: take by weighing 0.48 kilogram of nanometer selenium, 0.16 kilogram of nanometer copper, 0.16 kilogram of zinc methionine, 0.2 kilogram of methionine iron, 0.16 kilogram of manganese methionine, 0.04 kilogram of KI, 0.08 kilogram of cobalt chloride, V
A0.32 kilogram in powder, V
D0.32 kilogram in powder, V
E(0.4 kilogram of 20,000,000,000/g) 0.04 kilogram, land plaster mix first for 0.06 kilogram in powder, 0.1 kilogram of cellulase, active dry yeast bacterium; 0.4 kilogram of zeolite powder again behind the mixing mixes for the second time; Take by weighing 0.52 kilogram of powdered rice hulls again, for the third time mixing; Take by weighing 1.0 kilograms at last, mixing is premix once more, and mark is preserved subsequent use;
(2) fine fodder compound preparation in early stage: take by weighing 224 parts of corns, 24 parts in wheat bran, 48 parts of palm kernel meals, 24 parts of dregs of beans, 44 parts of sunflower cakes, 36 parts of DDGS, 2.4 parts of salt, 1.6 parts of calcium monohydrogen phosphates; Mix with whole premix; Be and fatten the early stage mixed concentrate and mix, it is subsequent use that mark is preserved;
(3) TRM preparation in early stage: take by weighing and be ready to 220 kilograms of corn stalk powders, maize storage 260 kilograms, hay 120 kg, mix, be 1000 kilograms and fatten rich selenium TRM in early stage with whole early stage mixed concentrates of fattening.
Fatten the preparation of rich selenium TRM of later stage:
(1) later stage premix preparation: take by weighing 0.6 kilogram of nanometer selenium, 0.2 kilogram of nanometer copper, 0.2 kilogram of zinc methionine, 0.25 kilogram of methionine iron, 0.2 kilogram of manganese methionine, 0.05 kilogram of KI, 0.1 kilogram of cobalt chloride, V
A0.04 kilogram in powder, V
D0.04 kilogram in powder, V
E(0.5 kilogram of 20,000,000,000/g) 0.05 kilogram, land plaster mix first for 0.075 kilogram in powder, 0.125 kilogram of cellulase, active dry yeast bacterium; 0.5 kilogram of zeolite powder again behind the mixing mixes for the second time; Take by weighing 1.0 kilograms of powdered rice hulls again, once more mixing; Take by weighing 0.9 kilogram of powdered rice hulls at last, mixing is premix once more, and mark is preserved subsequent use;
(2) fatten later stage fine fodder compound preparation: take by weighing 290 kilograms of corns, 60 kilograms in wheat bran, 20 kilograms in rice bran, 25 kilograms of dregs of beans, 55 kilograms of DDGS, 50 kilograms of oil cake of flax seed, 3 kilograms of salt, 2 kilograms of calcium monohydrogen phosphates; Mix with whole later stage premix; Be and fatten the later stage mixed concentrate, mark is preserved subsequent use;
(3) fatten later stage TRM preparation: claim 160 kilograms of corn stalk powders, 180 kilograms of maize storages, 170 kilograms in hay, mix, be 1000 kilograms and fatten rich selenium TRM of later stage with whole later stage mixed concentrates of fattening.
Embodiment 3
Preparation fatten early stage rich selenium TRM with fatten each 1000 kilograms of rich selenium TRMs of later stage, method is following:
Fatten the preparation of rich selenium TRM in early stage:
(1) premix preparation in early stage: take by weighing 0.44 kilogram of nanometer selenium, 0.18 kilogram of nanometer copper, 0.14 kilogram of zinc methionine, 0.22 kilogram of methionine iron, 0.14 kilogram of manganese methionine, 0.05 kilogram of KI, 0.07 kilogram of cobalt chloride, V
A0.4 kilogram in powder, V
D0.26 kilogram in powder, V
E(0.4 kilogram of 20,000,000,000/g) 0.04 kilogram, land plaster mix first for 0.07 kilogram in powder, 0.9 kilogram of cellulase, active dry yeast bacterium; 0.4 kilogram of zeolite powder again behind the mixing mixes for the second time; Take by weighing 0.65 kilogram of powdered rice hulls again, for the third time mixing; Take by weighing 1.0 kilograms at last, mixing is premix once more, and mark is preserved subsequent use;
(2) fine fodder compound preparation in early stage: take by weighing 220 parts of corns, 28 parts in wheat bran, 44 parts of palm kernel meals, 26 parts of dregs of beans, 42 parts of sunflower cakes, 38 parts of DDGS, 2.4 parts of salt, 1.6 parts of calcium monohydrogen phosphates; Mix with whole premix; Be and fatten the mixed concentrate in early stage, mark is preserved subsequent use;
(3) TRM preparation in early stage: take by weighing and be ready to 210 kilograms of corn stalk powders, 280 kilograms of maize storages, 100 kilograms in hay, mix, be 1000 kilograms and fatten rich selenium TRM in early stage with whole early stage mixed concentrates of fattening.
Fatten the preparation of rich selenium TRM of later stage:
(1) later stage premix preparation: take by weighing 0.55 kilogram of nanometer selenium, 0.23 kilogram of nanometer copper, 0.18 kilogram of zinc methionine, 0.27 kilogram of methionine iron, 0.18 kilogram of manganese methionine, 0.06 kilogram of KI, 0.85 kilogram of cobalt chloride, V
A0.05 kilogram in powder, V
D0.033 kilogram in powder, V
E(0.5 kilogram of 20,000,000,000/g) 0.05 kilogram, land plaster mix first for 0.078 kilogram in powder, 0.115 kilogram of cellulase, active dry yeast bacterium; 0.5 kilogram of zeolite powder again behind the mixing mixes for the second time; Take by weighing 1.0 kilograms of powdered rice hulls again, once more mixing; Take by weighing 1.15 kilograms of powdered rice hulls at last, mixing is premix once more, and mark is preserved subsequent use;
(2) fatten later stage fine fodder compound preparation: take by weighing 295 kilograms of corns, 55 kilograms in wheat bran, 23 kilograms in rice bran, 22 kilograms of dregs of beans, 58 kilograms of DDGS, 45 kilograms of oil cake of flax seed, 3 kilograms of salt, 2 kilograms of calcium monohydrogen phosphates; Mix with whole later stage premix; Be and fatten the later stage mixed concentrate, mark is preserved subsequent use;
(3) fatten later stage TRM preparation: claim 155 kilograms of corn stalk powders, 190 kilograms of maize storages, 165 kilograms in hay, mix, be 1000 kilograms and fatten rich selenium TRM of later stage with whole later stage mixed concentrates of fattening.