CN105494954A - Straw type dietary compound enzyme preparation and application thereof - Google Patents
Straw type dietary compound enzyme preparation and application thereof Download PDFInfo
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- CN105494954A CN105494954A CN201510889138.6A CN201510889138A CN105494954A CN 105494954 A CN105494954 A CN 105494954A CN 201510889138 A CN201510889138 A CN 201510889138A CN 105494954 A CN105494954 A CN 105494954A
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Abstract
The invention discloses a straw type dietary compound enzyme preparation. The enzyme preparation comprises components in parts by weight as follows: 55-110 parts of cellulose, 55-105 parts of beta-dextranase, 45-100 parts of xylanase, 45-110 parts of pectinase and 70-140 parts of laccase. According to the compound enzyme preparation, lignin, cellulose and hemicellulose of straw are degraded effectively after the straw is processed, surrounding of other nutrients by lignocellulose is broken through, so that the straw nutrient digestibility is improved and the feed conversion rate is increased. The compound enzyme preparation is convenient to use, and the animal growth rate, the economic benefit and the social benefit can be increased effectively.
Description
Technical field
The present invention relates to feed industry ruminant roughage processing and utilization field, be specifically related to a kind of stalk type diet complex enzyme formulation and application thereof.
Background technology
China's agricultural straw resource is very abundant, and within 2013, national agricultural crop straw total output is 9.64 hundred million tons, and account for that whole world stalk always produces 30%, first of the row world, can Collection utilization amount be 8.19 hundred million tons.Agricultural crop straw is also one of the abundantest feed resource, but for stock keeping less than 31%, still there is very large development potentiality.Stalk has the features such as high microsteping low quality, and its palatability is poor, utilization rate is low.Nonruminant generally can not utilize stalk, and only have a little part can be used to raise ruminant, ruminant also just only has 20%-30% to the digestibility of stalk.Therefore study convenience, economic, practical straw feed modulation technique, make full use of this feed resource, significant to development China save-grain animal husbandry.
The key of stalk resource fodder is the digestive utilization ratio improving stalk, and lignocellulosic is the barrier affecting straw biological utilization.Enzyme preparation is used for straw feed as a kind of Bioactive Substance Additive and produces to have caused with the nutritive value and quality that improve straw feed and pay close attention to widely, and countries in the world are developed one after another and produced fodder enzyme preparation in recent years.Enzyme preparation is nontoxic, noresidue, have no side effect, and is the novel growth-promoting class feed addictive of high-quality.Enzymatic isolation method Treating straw is that select can the single or compound enzyme of hydrocellulose, hemicellulose and lignin degrading, processes meeting under enzyme action condition, thus reduces fibrous substance ratio, improve the method for soluble sugar content to stalk.At present, both at home and abroad about the investigation and application of fodder enzyme preparation Treating straw in ruminant produces is more and more extensive.Enzyme preparation for farm crop straw organism process mainly contains cellulase, zytase, 1,4 beta-glucanase, Galactanase, mannase, the SNSPs such as pectase (NSP) enzyme.At present, studying more is use white rot fungus degrading lignin, then in conjunction with non-starch polysaccharide enzyme, ANFs SNSP in degrading straw, thus improve straw feed digestibility.
Therefore develop effective combination of enzyme preparations, removing in actual production the barrier affecting straw biological and utilize, is the effective way improving straw utilization rate.
Summary of the invention
The object of the present invention is to provide a kind of stalk type diet complex enzyme formulation, to improve the utilization rate of stalk in animal diet.
For reaching above object, the invention provides a kind of stalk type diet complex enzyme formulation, wherein, described enzyme preparation contains each component of following weight portion: cellulase 55-110 part, 1,4 beta-glucanase 55-105 part, zytase 45-100 part, pectase 45-110 part, laccase 70-140 part.
Wherein, described enzyme preparation contains each component of following weight portion: cellulase 65-90 part, 1,4 beta-glucanase 60-85 part, zytase 55-80 part, pectase 60-90 part, laccase 85-130 part.
Wherein, described enzyme preparation contains each component of following weight portion: cellulase 70-80 part, 1,4 beta-glucanase 65-75 part, zytase 60-70 part, pectase 70-80 part, laccase 90-120 part.
Wherein, described enzyme preparation comprises and also comprises carrier, and with the gross mass of described complex enzyme formulation for benchmark, the addition of described carrier is 50-75%.
In the present invention, the effect of carrier can carry active component exactly, improves that it is dispersed, improves its chemical stability and adsorptivity prevents mouldy raised material.When not affecting the addition of enzyme own, carrier can be one or more.
Optionally, described carrier is selected from one or more in wheat flour, maltodextrin, corncob and powdered rice hulls.The present invention has no particular limits for the part by weight of various carrier, can be the ratio that this area routine uses.
In order to obtain better dispersiveness, chemical stability and adsorptivity, preferably, described carrier is made up of wheat flour and maltodextrin, and the part by weight of wheat flour and maltodextrin is 1:1.
Present invention also offers the preparation method of described complex enzyme formulation, it is characterized in that, first various enzyme is mixed by weight and make complex enzyme, more described complex enzyme and carrier contact are mixed obtain described complex enzyme formulation.
Present invention also offers the application of described complex enzyme formulation in agricultural crop straw degraded.
Optionally, first described complex enzyme formulation is contacted with concentrate feed to mix and obtain compound 1, more described compound 1 and coarse fodder stalk are contacted with water mix and obtain feeding animals after TMR; Wherein, described concentrate feed comprises fine fodder and premix, and described coarse fodder stalk comprises at least one in maize straw, wheat stalk and rice straw.
Wherein, described premix is the abbreviation of additive premix, it is by one or more microcomponents (comprising the additives such as various trace quantity mineral substance element, various vitamin, synthesizing amino acid, some drugs) and diluent or carrier proportioning on request, the osculant compound feed products made after Homogeneous phase mixing.
Wherein, described TMR is the abbreviation of TotalMixedRations (TRM), so-called TRM (TMR) a kind ofly coarse fodder, fine fodder, mineral matter, vitamin and other additive fully to be mixed, and enough nutrition can be provided to meet the breeding technology of ruminant requirement.
Optionally, making concentration after being contacted with water by described complex enzyme formulation is 0.5-1.0% composite enzyme solution, feeding animals after described composite enzyme solution being mixed with stalk fodder.
Wherein, the addition of described complex enzyme formulation accounts for the 0.05-0.2 % by weight of animal diet total amount.
Stalk type diet complex enzyme formulation provided by the invention has the following advantages:
Agricultural crop straw is rich in cellulose, hemicellulose and lignin, network structure is closely become by crosslinked for whole cell membrane, add the mechanical strength of cell membrane and the resistivity to pathogen, also counteracts that ruminant tumor gastric microbial hydrolase contacts with hemicellulose with cellulose in cell membrane simultaneously, thus reduce the degradation efficiency of fibrous polysaccharaide.And the inside and outside 1,4 beta-glucanase degradable stalk cellulose of cutting in cellulase generates cellobiose and cell-oligosaccharide, then resolve into the available glucose of body through beta-glucosidase.The hemicellulase such as 1,4 beta-glucanase and zytase energy degradation of hemicellulose is various pentose, and reduces the water-soluble rear viscosity of hemicellulose.Zytase can destroy the lignin component in stalk further.Pectase can make the protective layer on plant top layer be damaged, and in body, probio can enter plant tissue and decompose fibre composition, improves the degradation rate of lignocellulosic.Laccase has degradation to lignin.Above-mentioned 3 class enzyme preparations act on cellulose, hemicellulose, lignin, pectin layer respectively, are convenient to probio in body and enter in plant cell in time and play a role; Destroy the structure of string, make cellulose conversion be utilizable carbohydrate, thus improve the degradation rate of lignocellulosic further.The present invention is by series of experimental research, and the combination determining cellulase+1,4 beta-glucanase+zytase+pectase+laccase is better to ligocellulose degradation's effect of stalk, and the availability of stalk is higher.
After complex enzyme formulation Treating straw provided by the invention, straw lignin, cellulose, hemicellulose are effectively degraded, and have broken the encirclement of lignocellulosic to other nutritional labelings, thus improve digestibility and the feed conversion rate of straw nutrition material.Complex enzyme formulation of the present invention is easy to use, can effectively improve growth of animal rate, economic benefit and social benefit.
Detailed description of the invention
Below will the present invention is described in detail by detailed description of the invention.
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.If do not specialize, the conventional means that in embodiment, technological means used is well known to those skilled in the art, is raw materials usedly commercial goods.
Described cellulase, >=10000U/g, Baiyin Sino Biotechnology Co., Ltd..
Described zytase, >=120000U/g, Baiyin Sino Biotechnology Co., Ltd..
Described 1,4 beta-glucanase, >=40000U/g, Baiyin Sino Biotechnology Co., Ltd..
Described pectase, >=10000U/g, Baiyin Sino Biotechnology Co., Ltd..
Described laccase, >=10000U/g, Baiyin Sino Biotechnology Co., Ltd..
Wherein, cellulase and dextranase are by the wooden mould production of Li Shi (long handle); Zytase is produced by pichia yeast; Pectase is produced by aspergillus niger; Laccase is produced by whiterot fungi.
Other carriers and feed addictive, raw material all can be bought from feed market and obtain.
Embodiment 1: the feeding effect checking of complex enzyme formulation degrading straw
1, test material
1.1 experimental animals: select 400 body weight to be that (26.95 ± 0.91kg), monthly age are close, the public lamb (non-castration) of the Du Bo that body condition is good × Small-fat-tail sheep first familiar generation is animal used as test.Test sheep is fed 2 times at 5:00 and 17:00 an every day respectively, freely drinks water.Scale of feeding adjusts according to sheep the previous day food-intake only, guarantees the surplus material having about 10% in feed trough.
1.2 test diets: prepare voluntarily, premixed feed is provided by Beijing Precise Animal Nutrient Research Center.According to mutton sheep nutritional need in NRC (2007), take corn stalk as roughage, preparation essence is slightly than the TMR diet of 4:6 and 5:5.The formal test phase is 66d, and first phase diet essence is thick than being 4:6, and second phase diet essence is thick than being 5:5, and concrete diet recipe is in table 1.
Table 1 feeding experiment diet composition and trophic level (dry matter basis: %)
Note: * premix provides for every kilogram of diet: VA13000IU, VD
34200IU, VE40mg; Iron 66mg, copper 13mg, manganese 60mg, zinc 60mg, selenium 0.3mg, iodine 0.5mg, cobalt 0.2mg.
1.3 test complex enzyme formulation compatibilities
The addition in different groups of enzyme preparation of the same race is equal, and carrier is made up of wheat flour and maltodextrin two kinds, and often the interior addition of group is equal.Diet per ton adds the complex enzyme formulation of 1kg.Enzyme is lived: cellulase >=10000U/g, zytase >=120000U/g, 1,4 beta-glucanase >=40000U/g, pectase >=10000U/g, laccase >=10000U/g.
2, test grouping
In test grouping, maize straw diet adds complex enzyme formulation proportioning in table 2.
Table 2 tests diet additive composition and addition (g)
3, testing index and method
Testing index: original body mass, terminates body weight, daily gain, inventory, surplus doses, diet and surplus material dry, dry matter intake, fine fodder consumption, coarse fodder consumption etc.
Original body mass and terminate body weight: preliminary trial period start and just the phase of trying terminate morning on the same day and raise before carry out claiming the empty body weight of every sheep.According to formal body weight numerical computations daily gain at whole story phase (ADG).
Accurately take every day and record in detail and often enclose inventory and surplus doses.Gather 1 feed and surplus material sample every 7d, measure its conventional nutrients.
4, data processing
Test data adopts Excel2007 to carry out edit, and adopt SAS8.1 statistical software ANOVA to carry out variance analysis, significant difference then carries out Multiple range test by DuncanShi method.P<0.05 is as the criterion of significant difference.
5, result
5.1 complex enzyme formulation Treating straws are on the impact of mutton sheep growth performance: in table 3.
The process of table 3 complex enzyme formulation is on the impact of mutton sheep growth performance
Note: colleague's numerical value letter not identical table shows significant difference (P<0.05).
Table 3 result shows: a test sheep starting weight difference not significantly (P>0.05), meets the principle of random packet.From dry matter intake, processed group I minimum, but each group difference is not significantly (P=0.107).Body weight, daily gain and feed-weight ratio difference is terminated all significantly (P<0.05) between each test group, wherein the end body weight of control group is significantly lower than rear 4 processed group II ~ V (P<0.05), there is no significant difference (P>0.05) with processed group I, then there is no significant difference (P>0.05) between 3 processed group III ~ V; The daily gain of processed group IV and processed group V is significantly higher than control group and processed group I, II (P<0.05), but do not have significant difference (P>0.05) between processed group IV and processed group V or between control group and processed group I, size order is processed group IV > processed group V > processed group III > processed group II > processed group I > control group; Each group of feed-weight ratio value size order is control group > processed group II > processed group I > processed group III > processed group V > processed group IV.That is, the daily gain of processed group IV and feed-weight ratio are distinguished the highest and minimum, be significantly higher than respectively and lower than control group, processed group I and II (P<0.05), there is no significant difference (P>0.05) with processed group V and processed group III.
Result illustrates: after complex enzyme formulation Treating straw diet provided by the invention, mutton sheep daily gain and feed conversion rate significantly improve, and degradation effect is better than control group and other processing with enzyme preparation groups.
The change of cost and economic benefit 5.2 complex enzyme formulation Treating straw is fed to mutton sheep: in table 4.
Table 4 feed cost and economic benefits comparison
| Project | Control group | Processed group III | Processed group IV | Processed group V |
| Test sheep number/only | 100 | 100 | 100 | 100 |
| Fattening time/d | 66 | 66 | 66 | 66 |
| Fine fodder consumption/kg | 3611.88 | 3367.59 | 3539.15 | 3567.84 |
| Coarse fodder consumption/kg | 4357.18 | 4057.78 | 4268.43 | 4302.27 |
| Diet total amount/t | 7.97 | 7.43 | 7.81 | 7.87 |
| Diet cost/unit | 12503.89 | 11654.80 | 12251.37 | 12350.14 |
| Enzyme preparation totle drilling cost/unit | 0.00 | 70.43 | 152.52 | 87.63 |
| Average total augment weight kg/ only | 9.25 | 9.56 | 11.25 | 10.56 |
| 1kg weightening finish fine fodder consumption/kg | 3.71 | 3.35 | 2.99 | 3.21 |
| 1kg weightening finish coarse fodder consumption/kg | 4.74 | 4.27 | 3.82 | 4.1 |
| 1kg increases weight feed cost/unit | 13.52 | 12.26 | 11.03 | 11.78 |
Note: feedstuff unit price (unit/kg), maize straw: 0.7, corn: 2.2, dregs of beans: 3.6, cotton dregs and DGGS:2.4, calcium bicarbonate: 2.17, salt: 1.15, premix: 7.5.
Table 4 result shows: each group 100 sheep feed 66d time required diet total weight range between 7.43 ~ 7.97t, cost is 11654.80 ~ 12503.89 yuan, and the scale of feeding of control group is the highest.At whole duration of test, the cost often organizing additive is respectively 0.00 yuan (control group), 70.43 yuan (processed group III), 87.63 yuan (processed group V) and 152.52 yuan (processed group IV).Compared with control group, processed group IV gaining effect preferably can reach 11.25kg.All there is larger difference in the required feed consumption of each group of mutton sheep per kilogram weightening finish, that is, feed cost when often organizing the weightening finish of mutton sheep per kilogram is also different, and wherein, control group is the highest, is 13.52 yuan, processed group IV minimum, is only 11.03 yuan.
Diet result shows: complex enzyme formulation provided by the invention effectively improves the availability of stalk, improves feed conversion rate and the price of deed, can obtain higher meat yield, remarkable in economical benefits under identical condition of feeding.
Stalk is the roughage resource that ruminant is enriched, the effective degradation results lignocellulosic of complex enzyme formulation provided by the invention, improve the availability of stalk, the more carbohydrate of ruminant tumor gastric microorganism can be supplied, improve microprotein output, promote the growth of animal, reduce feeding cost.
After complex enzyme formulation Treating straw provided by the invention, efficient solution is except ANFs such as lignin, cellulose, hemicelluloses, improve the digestibility of ruminant tumor gastric to straw nutrition material, thus improve growth of animal rate, feed conversion rate and economic benefit.Complex enzyme formulation of the present invention is easy to use, and directly can mix and feed in TMR, effectiveness comparison is obvious.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. a stalk type diet complex enzyme formulation, is characterized in that, described enzyme preparation contains each component of following weight portion: cellulase 55-110 part, 1,4 beta-glucanase 55-105 part, zytase 45-100 part, pectase 45-110 part, laccase 70-140 part.
2. complex enzyme formulation according to claim 1, it is characterized in that, described enzyme preparation contains each component of following weight portion: cellulase 65-90 part, 1,4 beta-glucanase 60-85 part, zytase 55-80 part, pectase 60-90 part, laccase 85-130 part.
3. complex enzyme formulation according to claim 2, it is characterized in that, described enzyme preparation contains each component of following weight portion: cellulase 70-80 part, 1,4 beta-glucanase 65-75 part, zytase 60-70 part, pectase 70-80 part, laccase 90-120 part.
4. according to the complex enzyme formulation in claim 1-3 described in any one, it is characterized in that, described enzyme preparation also comprises carrier, and with the gross mass of described complex enzyme formulation for benchmark, the addition of described carrier is 50-75%.
5. complex enzyme formulation according to claim 4, is characterized in that, described carrier is selected from one or more in wheat flour, maltodextrin, corncob and powdered rice hulls.
6. the preparation method of the complex enzyme formulation according to any one of claim 1-5, is characterized in that, is first mixed by weight by various enzyme and makes complex enzyme, more described complex enzyme and carrier contact is mixed and obtain described complex enzyme formulation.
7. the application of complex enzyme formulation described in any one in agricultural crop straw degraded in claim 1-5.
8. application according to claim 7, is characterized in that, is first contacted with concentrate feed to mix by described complex enzyme formulation to obtain compound 1, more described compound 1 and coarse fodder stalk is contacted to mix with water and obtain TMR feeding animals;
Wherein, described concentrate feed comprises fine fodder and premix, and described coarse fodder stalk comprises at least one in maize straw, wheat stalk and rice straw.
9. application according to claim 7, is characterized in that, making concentration after being contacted with water by described complex enzyme formulation is 0.5-1.0% composite enzyme solution, feeding animals after described composite enzyme solution being mixed with stalk fodder.
10. according to the application in claim 7-9 described in any one, it is characterized in that, the addition of described complex enzyme formulation accounts for the 0.05-0.2 % by weight of animal diet total amount.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106754824A (en) * | 2017-01-16 | 2017-05-31 | 深圳市龙城生物科技股份有限公司 | A kind of method of complex enzyme and its solution degrading straw |
| CN106804917A (en) * | 2017-03-24 | 2017-06-09 | 中国农业科学院特产研究所 | A kind of puffing straw feed and preparation method thereof |
| CN107027970A (en) * | 2017-04-27 | 2017-08-11 | 王琳琳 | A kind of method that utilization wheat straw prepares feed |
| CN108208347A (en) * | 2018-03-06 | 2018-06-29 | 新疆畜牧科学院饲料研究所 | A kind of enzyme bacterium mixture and method for reducing fiber content in cotton stalk |
| CN110353087A (en) * | 2019-07-11 | 2019-10-22 | 南京农业大学 | The preparation method and fermented feed of rape stalk fermented feed |
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| CN102726594A (en) * | 2012-06-04 | 2012-10-17 | 郝伟星 | High-efficiency multienzyme concentration forage |
| CN102742737A (en) * | 2012-05-08 | 2012-10-24 | 山西农业大学 | Selenium-rich total mixed ration for fattening sheep, and production method thereof |
| CN102870955A (en) * | 2012-09-13 | 2013-01-16 | 吉林省农业科学院 | Straw multienzyme microbial inoculum for livestock and poultry and preparation process |
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Patent Citations (3)
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| CN102742737A (en) * | 2012-05-08 | 2012-10-24 | 山西农业大学 | Selenium-rich total mixed ration for fattening sheep, and production method thereof |
| CN102726594A (en) * | 2012-06-04 | 2012-10-17 | 郝伟星 | High-efficiency multienzyme concentration forage |
| CN102870955A (en) * | 2012-09-13 | 2013-01-16 | 吉林省农业科学院 | Straw multienzyme microbial inoculum for livestock and poultry and preparation process |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754824A (en) * | 2017-01-16 | 2017-05-31 | 深圳市龙城生物科技股份有限公司 | A kind of method of complex enzyme and its solution degrading straw |
| CN106804917A (en) * | 2017-03-24 | 2017-06-09 | 中国农业科学院特产研究所 | A kind of puffing straw feed and preparation method thereof |
| CN107027970A (en) * | 2017-04-27 | 2017-08-11 | 王琳琳 | A kind of method that utilization wheat straw prepares feed |
| CN108208347A (en) * | 2018-03-06 | 2018-06-29 | 新疆畜牧科学院饲料研究所 | A kind of enzyme bacterium mixture and method for reducing fiber content in cotton stalk |
| CN108208347B (en) * | 2018-03-06 | 2021-06-01 | 新疆畜牧科学院饲料研究所 | Enzyme bacterium mixture and method for reducing fiber content in cotton stalks |
| CN110353087A (en) * | 2019-07-11 | 2019-10-22 | 南京农业大学 | The preparation method and fermented feed of rape stalk fermented feed |
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