CN104605162A - Application of aspergillus oryzae as beef cattle intestinal tract and excrement methane inhibitor - Google Patents
Application of aspergillus oryzae as beef cattle intestinal tract and excrement methane inhibitor Download PDFInfo
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- CN104605162A CN104605162A CN201510036906.3A CN201510036906A CN104605162A CN 104605162 A CN104605162 A CN 104605162A CN 201510036906 A CN201510036906 A CN 201510036906A CN 104605162 A CN104605162 A CN 104605162A
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Abstract
The invention discloses application of aspergillus oryzae as a beef cattle intestinal tract and excrement methane inhibitor. Aspergillus oryzae is added into a totally-mixed beef cattle fodder and the fodder is fed to beef cattle at the dosage of 8-12 mg/kg each day. The application of aspergillus oryzae as the beef cattle intestinal tract and excrement methane inhibitor has both environmental and economical benefits.
Description
Technical field
The present invention relates to Technology of Reducing Greenhouse Gas Emissions field, be specifically related to the application of aspergillus oryzae as beef cattle enteron aisle and ight soil methane inhibitor.
Background technology
Methane is a kind of important greenhouse gases, and its greenhouse effects are 20 ~ 30 times of carbon dioxide, and the influence of methane on global warming accounts for and allly affect 15% ~ 20% of climate warming factor effect.And wherein derive from ruminant greatly.Methane is the product in ruminant digestive processes, its stable chemical nature, and general being difficult to is digested and assimilated in vivo, and it mainly excretes in the mode of belch, and in addition, the methane content that ruminant ight soil produces also can not be ignored.The discharge of methane means losing of energy, and research shows, ruminant accounts for the energy that the form of methane is lost and takes in 2% ~ 15% of gross energy, therefore, has environment and economy double benefit to the research of ruminant methane inhibitor.
Along with the raising of people's living standard, to the demand of beef in continuous increase, therefore, steer ration quantity constantly increases, and the environmental problem brought thus also progressively receives the concern of people.Methane inhibitor common at present is mainly divided into polydentate compound, ion carrier compound, organic acid, a few class materials such as microorganism formulation, plant extracts, saponin and grease.All kinds of methane inhibitor is all in the research initial stage, and some methane inhibitor effect is unstable or destroy Rumen Internal Environment, and cause the drawbacks such as efficiency of feed utilization reduction, it is necessary for therefore researching and developing effective ruminant methane inhibitor.Aspergillus oryzae is the generally regarded as safe food-grade bacterial classification of FAO/WHO, be can Direct-fed to the microniological proudcts of animal, research in the past shows that aspergillus oryzae can improve the digestibility of fiber in dairy cow diet, but also has result inconsistent with it.Also have about the report of aspergillus oryzae as feed addictive in prior art, wherein, aspergillus oryzae is mainly through improving the fermenting property of ruminant tumor gastric thus improving production performance and the body condition of ruminant.And whether can reduce the discharge capacity of beef cattle enteron aisle and ight soil methane and the suitableeest additive effect about aspergillus oryzae, have not been reported.
Summary of the invention
The object of the invention is to by a certain amount of methane inhibitor of beef cattle supplementary feeding, thus reduce Feces of Beef Cattle and enteron aisle discharge of methane.
Object of the present invention can be achieved through the following technical solutions.
Aspergillus oryzae is as the application of beef cattle enteron aisle and ight soil methane inhibitor.
Using method: added to by aspergillus oryzae in beef cattle total mixed feed, every day, per weight dosage of feeding was 8-12mg/kg body weight.
Beneficial effect of the present invention: make the average daily methane production of its enteron aisle, per weight methane production and methane can reduce 18.77%, 20% and 18.73% respectively to the aspergillus oryzae of test beef cattle supplementary feeding every day 8-12mg/kgBW (body weight).In addition, the average daily emission flux of methane that test Feces of Beef Cattle is stacking (0-45d) in storage process is naturally made to reduce 55.55%.Aspergillus oryzae had environment and economy double benefit as the application of beef cattle enteron aisle and ight soil methane inhibitor.
Accompanying drawing explanation
Fig. 1 is ight soil discharge of methane speed variation diagram in time.
Detailed description of the invention
Aspergillus oryzae is on the impact of beef cattle enteron aisle and ight soil discharge of methane and production performance
1 materials and methods
1. the selection of 1 animal used as test and feeding and management
Test is carried out in Agricultural University Of He'nan Mao Zhuan practice base.Select body weight, monthly age, daily gain close, healthy anosis Simmental Cross Beef Cattle 8, is divided at random two groups (contrast, aspergillus oryzaes), ties raising, freely drink water, and each group 6:00 and 18:00 every day feeds and identical entirely mix daily ration.
1.2 experimental design
Feeding experiment was carried out in April, 2014-May, totally 37 days (7 day preliminary trial period just tried the phase in 30 days).Control group fed basal diet; Aspergillus oryzae group is fed the aspergillus oryzae additive of basal diet 8-12mg/kg body weight; That tests utilizes SF in last 5 days
6trace method determination test ox methane enteron aisle discharge capacity.
Ight soil discharge of methane test in May, 2014-July carries out, totally 45 days.In the feeding experiment later stage, the ight soil that collection control group and aspergillus oryzae group test ox drain, piling growth × wide × height is respectively the dunghill of 2.00 × 1.00 × 0.10m, storage in open system, the methane emission flux that two test group beef cattles void excreta is measured, each test group three measuring points by static chamber-gas chromatography.Stack first 9 day every day 8:30-9:00 and gather the methane emission flux of two groups of ight soil, every other day measure once afterwards.
1.3 test material
Methane inhibitor is aspergillus oryzae.
1.4 test determination index and methods
1.4.1 production performance
Average daily gain: preliminary trial period start with during off-test respectively land used pound measure the body weight of every ox, calculate average daily gain as follows.
Computing formula:
Feed intake: measured the feed intake of an ox every 5 days, meanwhile gathers complete mixed diet feed sample, measures dry matter content (70 DEG C), calculates the dry matter intake of on average every ox every day.
1.4.2 enteron aisle methane emission
1.4.2.1 gas collecting on-test, is by the SF of known infiltration rate
6osmos tube is put in cud, and the latter 5 day every day of formal phase utilizes self-control ox to grip the gas collected and test ox and breathe out, and is a cycle with 24h.The same day sent test determination SF back to by the sample collected
6and CH
4concentration, the discharge capacity according to following formulae discovery test Roll road methane:
（1）
In formula:
r cH4 -methane discharge of ruminant speed, L/d;
r sF6 -SF
6infiltration rate, mg/d;
6.518-SF
6density, kg/m
3;
[
cH 4 cH in]-sample gas
4concentration, 10
-6(molar fraction);
[
sF 6 sF in]-sample gas
6concentration, 10
-12(molar fraction).
1.4.2.2 gas concentration measuring
SF6 concentration determination:
Employing is furnished with the gas-chromatography of electron capture detector (ECD), 5 molecular sieve chromatography posts.Column temperature 50 DEG C, injector temperature 100 DEG C, detector temperature 250 DEG C.Sample size: 1mL.
CH4 concentration determination:
Employing is furnished with the gas-chromatography of hydrogen flame ionization detector (FID), PQ chromatography column.Column temperature 55 DEG C, injector temperature 150 DEG C, detector temperature 200 DEG C.Sample size: 1mL.
1.4.3 ight soil methane emission flux
1.4.3.1, during gas sampling sampling, buckle case lid, use water-stop.Respectively after buckle closure 0,10,20,30min time, gas is got from wireway with 50mL syringe, by in the overall plastic switch valve foil laminated film gas production bag of the gas inject 0.1L of collection, taken back the concentration of the methane of use for laboratory gas chromatographic analysis gaseous sample.Open case lid after sampling terminates, keep natural ventilating status.
Emission flux with following formulae discovery ight soil:
In formula: R is greenhouse gas emission flux (mg.m
-2.min
-1); M is gas molar quality (g.mol
-1); V
0for (0 DEG C, 1013hPa) molar volume of gas under standard state, (22.41 × 10
-3m
3. mol
-1); T
0and P
0be respectively absolute temperature (K) and the air pressure (hPa) of air under standard state; T and P
0actual temperature (K) when being respectively sampling in case and actual pressure (hPa); DC
t/ d
tfor the time dependent regression straight line slope of greenhouse gas concentration in case in observation time, C
tfor the volume mixture specific concentration (v/v) of gas tested in t case; T is time (min); H is sampling box height (m).
1.4.3.2 gas analysis sample utilizes chromatography of gases to measure methane content.Detector temperature (F1D) 200 DEG C; Injector temperature 120 DEG C; Column temperature 65 DEG C; Standard specimen concentration 20.1ppm.
1.4.3.3 environmental index measures
The actual pressure of test site is measured with air gauge, for calculating the emission flux of ight soil methane while gathering gas.And record the environment temperature of duration of test every 5min with automatic warm humidity measuring apparatus.
1.5 data processing
Data statistics adopts spss17.0 to carry out one-way analysis of variance to each group of data, and result all represents with average ± standard deviation (Mean+SD).
2 results and analysis
2.1 test ox daily gain and feed intakes
Ox daily gain tested by table 1
Note: same column shoulder note capitalization difference represent difference extremely significantly (
p<0.01), lowercase difference represent significant difference (
p<0.05), containing same letter and unreceipted expression difference not significantly (
p>0.05).
As can be seen from Table 1, the original body mass of control group and aspergillus oryzae group, end are heavy, the difference of average daily gain and dry matter intake all not significantly (p>0.05), but the average daily gain of aspergillus oryzae group and dry matter intake do not exceed 11.11% and 0.94% than contrast component.Illustrate that aspergillus oryzae is improved the trend of beef cattle average daily gain and dry matter intake.
2.2 test Roll road methane emissions
Test Niu Jun methane emission is in table 2.Can find out, control group and aspergillus oryzae group test the average daily methane production in Roll road, per weight methane production and methane can difference reach the pole level of signifiance (p<0.01), and the average daily methane production in aspergillus oryzae group test Roll road, per weight methane production and methane can reduce 18.77%, 20% and 18.73% than control group respectively.See thus, aspergillus oryzae has significant effect as beef cattle enteron aisle methane inhibitor.
Ox methane emission tested by table 2
| Index | Control group | Aspergillus oryzae group |
| Methane production L (headd) -1 | 418.90±23.79 Aa | 340.24±16.75 Bb |
| Per weight methane production Lkg -1 | 0.70±0.02 Aa | 0.56±0.01 Bb |
| Methane energy MJd -1 | 16.76±0.95 Aa | 13.62±0.67 Bb |
Note: same column shoulder note capitalization difference represent difference extremely significantly (
p<0.01), lowercase difference represent significant difference (
p<0.05), containing same letter and unreceipted expression difference not significantly (
p>0.05).
2.3 ight soil methane emission flux
Fig. 1 shows duration of test, ight soil discharge of methane speed variation diagram in time.In experimental period, the average daily temperature of external environment is 27.63 DEG C, and the change in fluctuation.Control group and the average daily emission flux of aspergillus oryzae group ight soil methane are respectively 2683.33 and 1165.86mgm
-2d
-1 .aspergillus oryzae group reduces 55.55% than the average daily emission flux of control group ight soil methane, and the two amplitude of variation shown along with the prolongation of pilling up time is inconsistent.The front 15d stacked, the two variation tendency is basically identical, but after 15d, discharge of methane peak appears in control group, and subsequently, methane emission flux progressively reduces.
3 brief summaries
In cattle diet, every day adds appropriate aspergillus oryzae (8-12mg/kg body weight), remarkable on the impact of the daily gain of beef cattle and dry matter intake, but the trend be improved; Aspergillus oryzae significantly can reduce the methane emission of beef cattle enteron aisle and ight soil.
Claims (2)
1. aspergillus oryzae is as the application of beef cattle enteron aisle and ight soil methane inhibitor.
2. aspergillus oryzae according to claim 1 is as the application of beef cattle enteron aisle and ight soil methane inhibitor, and it is characterized in that: added to by aspergillus oryzae in beef cattle total mixed feed, dosage of feeding every day is 8-12mg/kg body weight.
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Cited By (4)
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| CN106036080A (en) * | 2016-06-08 | 2016-10-26 | 中国农业科学院饲料研究所 | Methane regulating agent containing candida tropicalis and application of methane regulating agent |
| CN107144647A (en) * | 2017-04-25 | 2017-09-08 | 兰州大学 | The assay method of sheep methane emission is hidden under a kind of grazing condition |
| WO2019102279A1 (en) * | 2017-11-23 | 2019-05-31 | Biopremix Technologies Llc | Procedure for the production of a multiplier and modulator additive of the ruminal microbiote |
| CN111815073A (en) * | 2020-08-06 | 2020-10-23 | 内蒙古工业大学 | Grassland biomass prediction method and device, electronic equipment and storage medium |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036080A (en) * | 2016-06-08 | 2016-10-26 | 中国农业科学院饲料研究所 | Methane regulating agent containing candida tropicalis and application of methane regulating agent |
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| CN111815073A (en) * | 2020-08-06 | 2020-10-23 | 内蒙古工业大学 | Grassland biomass prediction method and device, electronic equipment and storage medium |
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Application publication date: 20150513 |