CN106036074A - Feed additive capable of preventing dairy cow subclinical mastitis, and preparation method of feed additive - Google Patents

Feed additive capable of preventing dairy cow subclinical mastitis, and preparation method of feed additive Download PDF

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Publication number
CN106036074A
CN106036074A CN201610605409.5A CN201610605409A CN106036074A CN 106036074 A CN106036074 A CN 106036074A CN 201610605409 A CN201610605409 A CN 201610605409A CN 106036074 A CN106036074 A CN 106036074A
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feed additive
parts
subclinical mastitis
raw material
feed
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郭晓亮
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Harbin Grace Feeding Natural Plant Co Ltd
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Harbin Grace Feeding Natural Plant Co Ltd
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Abstract

The invention discloses a feed additive capable of preventing dairy cow subclinical mastitis, and a preparation method of the feed additive. The feed additive capable of preventing the dairy cow subclinical mastitis is prepared from the following components in parts by weight: 25-35 parts of corn, 18-25 parts of bean pulp, 15-20 parts of fresh ginger, 15-20 parts of liquorice root, 5-12 parts of common selfheal fruit-spike, 5-8 parts of Indian mockstrawberry herb, 5-8 parts of herba artemisiae annuae and 1-4 parts of honeysuckle flower. The feed additive capable of preventing the dairy cow subclinical mastitis is balanced in nutrition, easy to prepare and lower in cost; the feed additive is prepared from pure traditional Chinese medicine components and does not contain any western medicine, thus being green, safe and environmental-friendly; after the feed additive is used, the occurrence of the dairy cow subclinical mastitis is effectively prevented; the nutrient substances of all the materials complement each other, so that the utilization rate of a feed is effectively improved, and good nutrition balance is realized while the cost is reduced; the result of a dairy cow feeding experiment proves that the functional feed provided by the invention can reduce number of somatic cells of dairy cows, so that the occurrence and development of the dairy cow subclinical mastitis are effectively prevented.

Description

A kind of feed additive preventing bovine subclinical mastitis and preparation method thereof
Technical field
The present invention relates to field of feed, a kind of feed additive preventing bovine subclinical mastitis and system thereof Preparation Method.
Background technology
Mastitis is a kind of multiple diseases that milch cow is common.Its pathogenic factor mainly due to the intrusion of pathogen, with And cause the various inducements of infection, as wound, Milking Methods And Techniques is the best, environmental health is bad, feeding and management is inappropriate, hormonal imbalance, Breast defect and other diseases induction etc..The pathogenic microorganism of mastitis is more complicated, including antibacterial, fungus, Mycoplasma, yeast Bacterium, virus etc. are up to kind more than 80.Change according to milk and latent mammitis and clinical type breast can be divided into or without clinical symptoms Scorching.Pathogen invades breast, does not causes clinical symptoms, and perusal breast, milk are without exception, but milk is biochemical and bacteriology On changed for latent mammitis;Breast, milk seen from naked eyes have occurred abnormal for clinic mastitis the most.According to it Changing different from general reaction degree, clinic mastitis can be divided into again mild, serious symptom and evil disease three class.The micro-life of different cause of diseases Clinical symptoms caused by thing difference, but fundamental symptoms is ill breast have hyperemia in various degree, increase, harden, temperature Heat and pain, lactogenic reduces or stops.Milk, initially without notable change, involves the secretory portion of mammary gland because of inflammation later, and milk becomes Thin, and have floccule or grumeleuse, visible pus and blood sometimes.When essence excretory duct and interstitial are involved, mammary gland can be sent out Raw necrosis.When between subcutaneous tissue and gland, connective tissue is encroached on, then in honeycomb knitting property latent mammitis, it and gangrenosum acne breast Inflammation is two types the most serious in mastitis.Suffer from seen from chronic cases district breast tissue elasticity lower, stiff, lactation amount subtracts Few, when secreting milk, milk turns to be yellow and thickening to some extent, sometimes has ziega, breast swelling.Some suffers from the mother of brucellosis Cattle, breast is very big, and does not has milking capacity.Latent mammitis i.e. Subclinical mastitis, perusal milch cow does not has inflammation to levy Wait, but milk inspection presents a kind of mastitis types of presentation of inflammatory change, the microorganism i.e. contained in milk and cell number Increase, the chemical characteristic of milk changes.It is characterized in that the ANOMALOUS VARIATIONS that cow breast and milk are visible by naked eyes, but newborn Juice changes in terms of physicochemical property, bacteriology.It is embodied in pH value more than 7.0, in meta-alkalescence;Sodium chloride contains Amount is more than 0.14%, and somatic number is at 500,000/more than mL;Bacterial population increases.It is currently mainly used antibiotic therapy Lac Bovis seu Bubali Fang Yan, conventional has penicillin, streptomycin, cephalosporin, cephalo-type, erythromycin etc..But the drug resistance of antibiotic and medicine are residual Stay and become inevitable problem.Relative to Western medicine, Chinese medicine derives from animals and plants and mineral, containing various active composition, is difficult to Produce drug resistance, noresidue.In addition the raising of people's living standard, the pursuit to natural green food is day by day strong, medium-height grass Medicine becomes a new approach for the treatment of cattle latent mammitis.
Summary of the invention
It is an object of the invention to provide a kind of feed additive preventing bovine subclinical mastitis and preparation method thereof, with The problem solving to propose in above-mentioned background technology.
For achieving the above object, the present invention provides following technical scheme:
A kind of feed additive preventing bovine subclinical mastitis, according to the raw material of weight portion be: Semen Maydis 25-35 part, bean Dregs of rice 18-25 part, Rhizoma Zingiberis Recens 15-20 part, Radix Glycyrrhizae 15-20 part, Spica Prunellae 5-12 part, Herba Duchesneae Indicae 5-8 part, Herba Artemisiae Annuae 5-8 part, Flos Lonicerae 1-4 Part.
As the further scheme of the present invention: the feed additive of described prevention bovine subclinical mastitis, according to weight portion Component be: Semen Maydis 28-32 part, bean cake 21-22 part, Rhizoma Zingiberis Recens 16-19 part, Radix Glycyrrhizae 16-19 part, Spica Prunellae 8-9 part, Herba Duchesneae Indicae 6-7 Part, Herba Artemisiae Annuae 6-7 part, Flos Lonicerae 2-3 part.
As the further scheme of the present invention: the feed additive of described prevention bovine subclinical mastitis, according to weight portion Component be: Semen Maydis 30 parts, bean cake 22 parts, 18 parts of Rhizoma Zingiberis Recens, 18 parts of Radix Glycyrrhizae, Spica Prunellae 9 parts, Herba Duchesneae Indicae 7 parts, Herba Artemisiae Annuae 7 parts, Flos Lonicerae 3 parts.
The preparation method of the feed additive of described prevention bovine subclinical mastitis, concretely comprises the following steps:
(1) screening: Semen Maydis, bean cake, Rhizoma Zingiberis Recens, Radix Glycyrrhizae, Spica Prunellae, Herba Duchesneae Indicae, Herba Artemisiae Annuae, Flos Lonicerae are carried out pesticide residues and take out Inspection, selects sampling observation qualified and does raw material without obvious pest and disease damage;
(2) dry in the sun: qualified raw material will be checked to be positioned over temperature 25 DEG C, humidity less than 30%, in the environment of clean hygiene, Carry out natural drying process, until the water content in raw material reaches 20-30%;
(3) pulverize: use super micron mill by granule that raw material pulverizing is fineness 400-800 mesh;
(4) packaging: the granule crushed is packed with plastic bag sealing nontoxic, good airproof performance, obtains feed additive, It is placed in the shady and cool place of being dried to store;
(5) inspection: feed additive is tested according to GB 13078.1-2006 forage health standard;
(6) dispensing: the ratio that feed additive is thrown in 80 grams according to every kilogram of feed is mixed into complete feed, and stirring is all Even feed.
As the further scheme of the present invention: until the water content in raw material reaches to 25% in concrete steps (2).
As the further scheme of the present invention: using super micron mill in concrete steps (3) is fineness 600 by raw material pulverizing Purpose granule.
The property of medicine and the drug effect of various raw material of the present invention are as follows:
The functions such as Semen Maydis sweet in the mouth is put down, and has in tune and whets the appetite, lung benefiting mind calming, clearing away damp-heat, profit liver and gall, slow down aging;Semen Maydis In abundant tryptophan, may participate in the renewal of plasma proteins in animal body, and riboflavin can be promoted to play a role, additionally aid Nicotinic acid and the synthesis of haemachrome, can dramatically increase pregnant animal tire son's internal antibody, and when poultry lack tryptophan, growth stops Stagnant, weight loss, Fat Accumulation reduces, sire atrophy of testis.Tryptophan can make animal globulin content increase, thus strengthens Premunition.
The protein content of bean cake accounts for 40%~48%, lysine 2.5%~3.0%, tryptophan 0.6%~0.7%, egg Propylhomoserin 0.5%~0.7%, has raising palatability, improves nutrient digestion and absorbs, and promotes growth, reduces the effect suffered from diarrhoea.
Gingerol in Rhizoma Zingiberis Recens has preferable antioxidation, can remove free radical in organism significantly (hydroxyl, super Oxygen-derived free radicals), reduce lipid peroxide content in liver cerebral tissue, gingerol is than vitamin E and butylhydroxy Fructus Foeniculi The anti-oxidation efficacy of ether is strong, and the curcumin in Rhizoma Zingiberis Recens can play the work of protection gastric mucosa by stimulating mucinous secretion simultaneously With.
Radix Glycyrrhizae is pulse family herbaceos perennial, and its root and root stock can be as medical materials, and its sweet in the mouth, gas are flat, property is delayed, and have The effect such as heat-clearing and toxic substances removing, logical muscle arteries and veins, profit vim and vigour, eliminating phlegm and stopping cough, QI invigorating stomach function regulating, coordinating the actions of various ingredients in a prescription, modern study shows, Radix Glycyrrhizae contains The plurality of active ingredients such as triterpenes, flavonoid, polysaccharide, Coumarins, alkaloids and aminoacid, have antiviral, antiulcer, The effects such as antitumor, antioxidation, removing toxic substances protect the liver, most important to animal health g and D.
Spica Prunellae purging liver-fire, improving eyesight, mass dissipating and swelling eliminating, Spica Prunellae can effectively reduce blood sugar concentration, improve carbohydrate tolerance, increase Liver glycogen synthesizes, and also has good antiinflammatory and antagonism immunologic function simultaneously.
Herba Duchesneae Indicae heat clearing away, removing heat from blood, subsiding a swelling, detoxify, in Herba Duchesneae Indicae, flavonoid and triterpenoid compound have antitumor and antioxidation Effect.
Herba Artemisiae Annuae clearind deficient heat, except hectic fever due to YIN-deficiency, solve summer-heat, preventing the attack (or recurrence) of malaria, jaundice eliminating, arteannuin in Herba Artemisiae Annuae and dihydroartemisinine to various inflammation Disease has good curative effect.
Flos Lonicerae is cold in nature, sweet in the mouth, enters lung, the heart, stomach warp, has heat-clearing and toxic substances removing, antiinflammatory, effect of qi-restoratives treatment wind.
Compared with prior art, the invention has the beneficial effects as follows:
The feed additive of the prevention bovine subclinical mastitis of the present invention is balanced in nutrition, preparation is simple, cost is relatively low, pure in Medicine composition, adds without any Western medicine, green safety environmental protection, can effectively prevent the generation of bovine subclinical mastitis, each storeroom Nutrient substance be complementary to one another, efficiency of feed utilization can be effectively improved, achieve good nutrition while reducing cost equal Weighing apparatus, Cow-feeding result of the test shows, the functional feed of the present invention can reduce the somatic cell count of milch cow, can effectively prevent milk The generation of cattle latent mammitis, development.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the technical scheme of this patent is described in more detail.
Embodiment 1
A kind of feed additive preventing bovine subclinical mastitis, according to the raw material of weight portion be: Semen Maydis 25 parts, bean cake 18 Part, 15 parts of Rhizoma Zingiberis Recens, 15 parts of Radix Glycyrrhizae, Spica Prunellae 5 parts, Herba Duchesneae Indicae 5 parts, Herba Artemisiae Annuae 5 parts, Flos Lonicerae 1 part.
The preparation method of the feed additive of described prevention bovine subclinical mastitis, concretely comprises the following steps:
(1) screening: Semen Maydis, bean cake, Rhizoma Zingiberis Recens, Radix Glycyrrhizae, Spica Prunellae, Herba Duchesneae Indicae, Herba Artemisiae Annuae, Flos Lonicerae are carried out pesticide residues and take out Inspection, selects sampling observation qualified and does raw material without obvious pest and disease damage;
(2) dry in the sun: qualified raw material will be checked to be positioned over temperature 25 DEG C, humidity less than 30%, in the environment of clean hygiene, Carry out natural drying process, until the water content in raw material reaches to 20%;
(3) pulverize: use super micron mill by granule that raw material pulverizing is fineness 400 mesh;
(4) packaging: the granule crushed is packed with plastic bag sealing nontoxic, good airproof performance, obtains feed additive, It is placed in the shady and cool place of being dried to store;
(5) inspection: feed additive is tested according to GB 13078.1-2006 forage health standard;
(6) dispensing: the ratio that feed additive is thrown in 80 grams according to every kilogram of feed is mixed into complete feed, and stirring is all Even feed.
Embodiment 2
A kind of feed additive preventing bovine subclinical mastitis, according to the raw material of weight portion be: Semen Maydis 28 parts, bean cake 21 Part, 16 parts of Rhizoma Zingiberis Recens, 16 parts of Radix Glycyrrhizae, Spica Prunellae 8 parts, Herba Duchesneae Indicae 6 parts, Herba Artemisiae Annuae 6 parts, Flos Lonicerae 2 parts.
The preparation method of the feed additive of described prevention bovine subclinical mastitis, concretely comprises the following steps:
(1) screening: Semen Maydis, bean cake, Rhizoma Zingiberis Recens, Radix Glycyrrhizae, Spica Prunellae, Herba Duchesneae Indicae, Herba Artemisiae Annuae, Flos Lonicerae are carried out pesticide residues and take out Inspection, selects sampling observation qualified and does raw material without obvious pest and disease damage;
(2) dry in the sun: qualified raw material will be checked to be positioned over temperature 25 DEG C, humidity less than 30%, in the environment of clean hygiene, Carry out natural drying process, until the water content in raw material reaches to 20%;
(3) pulverize: use super micron mill by granule that raw material pulverizing is fineness 400 mesh;
(4) packaging: the granule crushed is packed with plastic bag sealing nontoxic, good airproof performance, obtains feed additive, It is placed in the shady and cool place of being dried to store;
(5) inspection: feed additive is tested according to GB 13078.1-2006 forage health standard;
(6) dispensing: the ratio that feed additive is thrown in 80 grams according to every kilogram of feed is mixed into complete feed, and stirring is all Even feed.
Embodiment 3
A kind of feed additive preventing bovine subclinical mastitis, according to the raw material of weight portion be: Semen Maydis 30 parts, bean cake 22 Part, 18 parts of Rhizoma Zingiberis Recens, 18 parts of Radix Glycyrrhizae, Spica Prunellae 9 parts, Herba Duchesneae Indicae 7 parts, Herba Artemisiae Annuae 7 parts, Flos Lonicerae 3 parts.
The preparation method of the feed additive of described prevention bovine subclinical mastitis, concretely comprises the following steps:
(1) screening: Semen Maydis, bean cake, Rhizoma Zingiberis Recens, Radix Glycyrrhizae, Spica Prunellae, Herba Duchesneae Indicae, Herba Artemisiae Annuae, Flos Lonicerae are carried out pesticide residues and take out Inspection, selects sampling observation qualified and does raw material without obvious pest and disease damage;
(2) dry in the sun: qualified raw material will be checked to be positioned over temperature 25 DEG C, humidity less than 30%, in the environment of clean hygiene, Carry out natural drying process, until the water content in raw material reaches to 25%;
(3) pulverize: use super micron mill by granule that raw material pulverizing is fineness 600 mesh;
(4) packaging: the granule crushed is packed with plastic bag sealing nontoxic, good airproof performance, obtains feed additive, It is placed in the shady and cool place of being dried to store;
(5) inspection: feed additive is tested according to GB 13078.1-2006 forage health standard;
(6) dispensing: the ratio that feed additive is thrown in 80 grams according to every kilogram of feed is mixed into complete feed, and stirring is all Even feed.
Embodiment 4
A kind of feed additive preventing bovine subclinical mastitis, according to the raw material of weight portion be: Semen Maydis 32 parts, bean cake 22 Part, 19 parts of Rhizoma Zingiberis Recens, 19 parts of Radix Glycyrrhizae, Spica Prunellae 9 parts, Herba Duchesneae Indicae 7 parts, Herba Artemisiae Annuae 7 parts, Flos Lonicerae 3 parts.
The preparation method of the feed additive of described prevention bovine subclinical mastitis, concretely comprises the following steps:
(1) screening: Semen Maydis, bean cake, Rhizoma Zingiberis Recens, Radix Glycyrrhizae, Spica Prunellae, Herba Duchesneae Indicae, Herba Artemisiae Annuae, Flos Lonicerae are carried out pesticide residues and take out Inspection, selects sampling observation qualified and does raw material without obvious pest and disease damage;
(2) dry in the sun: qualified raw material will be checked to be positioned over temperature 25 DEG C, humidity less than 30%, in the environment of clean hygiene, Carry out natural drying process, until the water content in raw material reaches to 30%;
(3) pulverize: use super micron mill by granule that raw material pulverizing is fineness 800 mesh;
(4) packaging: the granule crushed is packed with plastic bag sealing nontoxic, good airproof performance, obtains feed additive, It is placed in the shady and cool place of being dried to store;
(5) inspection: feed additive is tested according to GB 13078.1-2006 forage health standard;
(6) dispensing: the ratio that feed additive is thrown in 80 grams according to every kilogram of feed is mixed into complete feed, and stirring is all Even feed.
Embodiment 5
A kind of feed additive preventing bovine subclinical mastitis, according to the raw material of weight portion be: Semen Maydis 35 parts, bean cake 25 Part, 20 parts of Rhizoma Zingiberis Recens, 20 parts of Radix Glycyrrhizae, Spica Prunellae 12 parts, Herba Duchesneae Indicae 8 parts, Herba Artemisiae Annuae 8 parts, Flos Lonicerae 4 parts.
The preparation method of the feed additive of described prevention bovine subclinical mastitis, concretely comprises the following steps:
(1) screening: Semen Maydis, bean cake, Rhizoma Zingiberis Recens, Radix Glycyrrhizae, Spica Prunellae, Herba Duchesneae Indicae, Herba Artemisiae Annuae, Flos Lonicerae are carried out pesticide residues and take out Inspection, selects sampling observation qualified and does raw material without obvious pest and disease damage;
(2) dry in the sun: qualified raw material will be checked to be positioned over temperature 25 DEG C, humidity less than 30%, in the environment of clean hygiene, Carry out natural drying process, until the water content in raw material reaches to 30%;
(3) pulverize: use super micron mill by granule that raw material pulverizing is fineness 800 mesh;
(4) packaging: the granule crushed is packed with plastic bag sealing nontoxic, good airproof performance, obtains feed additive, It is placed in the shady and cool place of being dried to store;
(5) inspection: feed additive is tested according to GB 13078.1-2006 forage health standard;
(6) dispensing: the ratio that feed additive is thrown in 80 grams according to every kilogram of feed is mixed into complete feed, and stirring is all Even feed.
Experimental test
Test group is that the feed additive of the prevention bovine subclinical mastitis of embodiment 3 preparation is thrown according to every kilogram of feed The ratio of 80 grams is mixed into complete feed;Matched group is complete feed.
Test site: cattle farm, Inner Mongol Huhehaote City.
Packet: select 100 healthy earlier lactating period or the Holstein cow in mid-term, according to parity, body weight, lactogenic natural law, The principle pairing that daily yielding is close is divided into 2 groups, experimental group and matched group.Packet situation is as shown in table 1.
Feeding and management: test group is consistent with feed mode with the daily ration structure of matched group.Test point preliminary trial period and just trying the phase, 7 days preliminary trial periods, matched group presses existing feeding regime, and test group is thrown something and fed test group daily ration, feeds at twice every day.The just phase of trying is 30 My god.Milch cow is loose housing, three feedings, milks, freely drinks water for three times.Body in on-test and end detection Lac Bovis seu Bubali respectively Cell number.
Milch cow packet situation tested by table 1
Test group declines notable than matched group somatic cell.Show that the feed additive that the present invention provides can effectively prevent milk The generation of Mammitis of cattle, development.
Above the better embodiment of this patent is explained in detail, but this patent is not limited to above-mentioned embodiment party Formula, in the ken that one skilled in the relevant art is possessed, it is also possible on the premise of without departing from this patent objective Make a variety of changes.

Claims (6)

1. the feed additive preventing bovine subclinical mastitis, it is characterised in that according to the raw material of weight portion be: Semen Maydis 25-35 part, bean cake 18-25 part, Rhizoma Zingiberis Recens 15-20 part, Radix Glycyrrhizae 15-20 part, Spica Prunellae 5-12 part, Herba Duchesneae Indicae 5-8 part, Herba Artemisiae Annuae 5-8 part, Flos Lonicerae 1-4 part.
The feed additive of prevention bovine subclinical mastitis the most according to claim 1, it is characterised in that described prevention milk The feed additive of cattle latent mammitis, according to the component of weight portion be: Semen Maydis 28-32 part, bean cake 21-22 part, Rhizoma Zingiberis Recens 16-19 Part, Radix Glycyrrhizae 16-19 part, Spica Prunellae 8-9 part, Herba Duchesneae Indicae 6-7 part, Herba Artemisiae Annuae 6-7 part, Flos Lonicerae 2-3 part.
The feed additive of prevention bovine subclinical mastitis the most according to claim 1 and 2, it is characterised in that described pre- The feed additive of anti-bovine subclinical mastitis, according to the component of weight portion be: Semen Maydis 30 parts, bean cake 22 parts, 18 parts of Rhizoma Zingiberis Recens, sweet Grass 18 parts, Spica Prunellae 9 parts, Herba Duchesneae Indicae 7 parts, Herba Artemisiae Annuae 7 parts, Flos Lonicerae 3 parts.
4. a preparation method for the feed additive of the prevention bovine subclinical mastitis as described in claim 1-3 is arbitrary, its It is characterised by, concretely comprises the following steps:
(1) screening: Semen Maydis, bean cake, Rhizoma Zingiberis Recens, Radix Glycyrrhizae, Spica Prunellae, Herba Duchesneae Indicae, Herba Artemisiae Annuae, Flos Lonicerae are carried out pesticide residues sampling observation, choosing Qualified with sampling observation and do raw material without obvious pest and disease damage;
(2) dry in the sun: by checking qualified raw material to be positioned over temperature 25 DEG C, humidity less than 30%, in the environment of clean hygiene, carry out Natural drying processes, until the water content in raw material reaches 20-30%;
(3) pulverize: use super micron mill by granule that raw material pulverizing is fineness 400-800 mesh;
(4) packaging: the granule crushed is packed with plastic bag sealing nontoxic, good airproof performance, obtains feed additive, juxtaposition Store in the cool place place of being dried;
(5) inspection: feed additive is tested according to GB 13078.1-2006 forage health standard;
(6) dispensing: the ratio that feed additive is thrown in 80 grams according to every kilogram of feed is mixed into complete feed, stirs and raises Hello.
The preparation method of the feed additive of prevention bovine subclinical mastitis the most according to claim 4, it is characterised in that Until the water content in raw material reaches to 25% in concrete steps (2).
The preparation method of the feed additive of prevention bovine subclinical mastitis the most according to claim 4, it is characterised in that Concrete steps (3) use super micron mill by granule that raw material pulverizing is fineness 600 mesh.
CN201610605409.5A 2016-07-28 2016-07-28 Feed additive capable of preventing dairy cow subclinical mastitis, and preparation method of feed additive Pending CN106036074A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112655828A (en) * 2020-12-14 2021-04-16 内蒙古农业大学 Cow feed additive and preparation method and application thereof

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN105410368A (en) * 2015-11-22 2016-03-23 衡阳市富矿饲料添加剂有限公司 Feed additive for preventing dairy cattle subclinical mastitis and preparation method thereof

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Publication number Priority date Publication date Assignee Title
CN105410368A (en) * 2015-11-22 2016-03-23 衡阳市富矿饲料添加剂有限公司 Feed additive for preventing dairy cattle subclinical mastitis and preparation method thereof

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四川农业科技与教育研究编委会: "《四川农业科技与教育研究》", 31 October 2005, 四川科学技术出版社 *

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Application publication date: 20161026