CN106029886B - 基因组整合的方法 - Google Patents

基因组整合的方法 Download PDF

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Publication number
CN106029886B
CN106029886B CN201480075698.1A CN201480075698A CN106029886B CN 106029886 B CN106029886 B CN 106029886B CN 201480075698 A CN201480075698 A CN 201480075698A CN 106029886 B CN106029886 B CN 106029886B
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nucleic acid
host cell
selectable marker
cell
nuclease
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CN106029886A (zh
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安德鲁·霍维茨
克里斯蒂·米歇尔·霍金斯
马克斯·舒伯特
韦恩·司徒
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Amyris Inc
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure

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CN201480075698.1A 2013-12-19 2014-12-19 基因组整合的方法 Expired - Fee Related CN106029886B (zh)

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US201361918625P 2013-12-19 2013-12-19
US61/918,625 2013-12-19
US201461937444P 2014-02-07 2014-02-07
US61/937,444 2014-02-07
PCT/US2014/071693 WO2015095804A1 (en) 2013-12-19 2014-12-19 Methods for genomic integration

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CN106029886A CN106029886A (zh) 2016-10-12
CN106029886B true CN106029886B (zh) 2021-02-05

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EP (1) EP3083958B1 (https=)
JP (1) JP6652489B2 (https=)
KR (1) KR102274445B1 (https=)
CN (1) CN106029886B (https=)
AU (1) AU2014368982B2 (https=)
CA (1) CA2933902C (https=)
MX (1) MX2016007797A (https=)
PT (1) PT3083958T (https=)
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