CN105969593A - Method for producing composite fruit wine from mulberries - Google Patents

Method for producing composite fruit wine from mulberries Download PDF

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CN105969593A
CN105969593A CN201610575427.3A CN201610575427A CN105969593A CN 105969593 A CN105969593 A CN 105969593A CN 201610575427 A CN201610575427 A CN 201610575427A CN 105969593 A CN105969593 A CN 105969593A
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mulberry
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王毓宁
吴彤东
陆皓茜
马佳佳
隋思瑶
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Suzhou Academy of Agricultural Sciences
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    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12HPASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/003Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages by a biochemical process

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Abstract

本发明公开了一种利用果桑生产的复合型果酒的方法,所述的复合型果酒由原料桑葚、桑叶和桑枝组合发酵而成,其中原料中桑葚、桑叶和桑枝的用量比为10:1~1.5:0.1~0.3;本发明选用果桑上的各个组份作为酿果酒的原料,不仅充分利用了果桑药用价值,而且组合连用的桑葚、桑叶和桑枝,发酵产生酒精,更有利于桑叶和桑枝的醇溶性有效成分溶出,发酵完成后,最终产品的中的有效成分:黄酮类、多酚类和花色苷的含量均产生了一定程度的提高;在发酵过程中,采用的是分段发酵的方法,避免了过量发酵的问题,提高了最终果酒中有效成分的含量。The invention discloses a method for producing compound fruit wine by using fruit mulberries. The compound fruit wine is fermented from raw materials mulberry, mulberry leaves and mulberry branches, wherein the ratio of the amount of mulberry, mulberry leaves and mulberry branches in the raw materials to It is 10:1~1.5:0.1~0.3; the present invention selects each component on fruit mulberry as the raw material of brewing fruit wine, not only fully utilizes the medicinal value of fruit mulberry, but also combines mulberry, mulberry leaf and mulberry branch, fermented The production of alcohol is more conducive to the dissolution of alcohol-soluble active ingredients in mulberry leaves and mulberry branches. After the fermentation is completed, the active ingredients in the final product: the contents of flavonoids, polyphenols and anthocyanins have all increased to a certain extent; During the fermentation process, the staged fermentation method is adopted, which avoids the problem of excessive fermentation and increases the content of active ingredients in the final fruit wine.

Description

一种利用果桑生产的复合型果酒的方法 A kind of method of the compound fruit wine that utilizes fruit mulberry to produce

技术领域 technical field

本发明涉及果桑的应用技术领域,尤其涉及一种利用果桑生产的复合型果酒的方法。 The invention relates to the technical field of application of fruit mulberries, in particular to a method for producing compound fruit wine using fruit mulberries.

背景技术 Background technique

果桑,是以结果为主,果叶兼用果桑的统称,具有极其丰富的营养和多种医疗保健功能,而且口味鲜美,自古以来深受人们的喜爱和追捧。桑葚又叫桑果、桑枣,味甜汁多,早在两千多年前,已是皇帝御用的补品,被称为“民间圣果”。现代研究分析证实,它含有丰富的活性蛋白、维生素、胡萝卜素、矿物质和17种氨基酸等,营养丰富,具有多种功效,被医学界誉为“21世纪的最佳保健果品”;1993年被国家卫生部公布为“既是食品也是药品”。桑葚的保健功能在《本草拾遗》、《本草经疏》、《中国大药典》、《随息居饮食谱》、《抗衰老中药学》等医典中均有记载。据《本草纲目》记载,具有“通血气、止消渴、利五脏关节,安心镇神,利水气消肿。”等功效。 Fruit mulberry is the general designation of fruit mulberry, which is mainly based on fruit and leaves. It has extremely rich nutrition and multiple medical and health care functions, and it tastes delicious. It has been loved and sought after by people since ancient times. Mulberry is also called mulberry fruit and mulberry jujube. It has a sweet taste and a lot of juice. As early as more than two thousand years ago, it was a tonic used by the emperor, and it was called "the holy fruit among the people". Modern research analysis has confirmed that it is rich in active protein, vitamins, carotene, minerals and 17 kinds of amino acids, etc. Announced by the Ministry of Health as "both food and medicine". The health care function of mulberry is recorded in medical classics such as "Supplements to Materia Medica", "Book of Materia Medica", "Chinese Pharmacopoeia", "Suixiju Diet Spectrum", "Anti-aging Chinese Materia Medica". According to the records of "Compendium of Materia Medica", it has the functions of "passing the blood and qi, quenching thirst, benefiting the five internal organs and joints, calming the mind and calming the mind, promoting water and gas to reduce swelling." and other effects.

祖国医学认为,桑葚性味甘寒,具有补肝益肾、生津润肠、乌发明目,补血助眠等功效。桑葚具有免疫促进作用。桑葚花青素属黄酮类衍生物,对心血管系统具有保健作用。桑葚对脾脏有增重作用,对溶血性反应有增强作用,可防止人体动脉硬化、骨骼关节硬化,促进新陈代谢。它可以促进血红细胞的生长,防止白细胞减少,并对治疗糖尿病、贫血、高血压、高血脂、冠心病、神经衰弱等病症具有辅助功效。桑葚可以改善皮肤(包括头皮)血液供应,营养肌肤,使皮肤白嫩及乌发等作用,并能延缓衰老。常食桑葚可以明目,缓解眼睛疲劳干涩的症状,能促进胃液分泌,刺激肠蠕动及解除燥热。桑葚是中老年人健体美颜、抗衰老的佳果与良药。 Chinese medicine believes that mulberry is sweet and cold in nature and has the functions of nourishing liver and kidney, promoting body fluid and moistening intestines, blackening eyes, nourishing blood and helping sleep. Mulberries have an immune-boosting effect. Mulberry anthocyanins are flavonoid derivatives, which have a health care effect on the cardiovascular system. Mulberry has a weight-increasing effect on the spleen, enhances the hemolytic reaction, prevents human arteriosclerosis, bone and joint sclerosis, and promotes metabolism. It can promote the growth of red blood cells, prevent leukopenia, and has auxiliary effects on the treatment of diabetes, anemia, hypertension, hyperlipidemia, coronary heart disease, neurasthenia and other diseases. Mulberries can improve the blood supply of the skin (including the scalp), nourish the skin, make the skin white and tender and black hair, and delay aging. Eating mulberries regularly can improve eyesight, relieve eye fatigue and dryness, promote gastric secretion, stimulate intestinal peristalsis and relieve dryness and heat. Mulberry is a good fruit and good medicine for middle-aged and elderly people's health, beauty and anti-aging.

桑叶是桑科植物桑的干燥叶,是蚕的日常食物;桑叶内有多种维生素及矿物质、氨基酸、碳水化合物和植物纤维。现代科学表明, 桑叶是上好的功能食品,它能降压、降脂、抗衰老、增加耐力、降低胆固醇、抑制 脂肪积累、抑制血栓生成,抑制肠内有害细菌繁殖,抑制有害的氧化物生成,最突出的功能是防止糖尿病; 据现代研究,干桑叶中含粗蛋白25-45%,碳水化合物20-25%,粗脂肪5%,以及丰富的钾、钙和维生素C、B1、B2、A等,还有各种微量元素铜、锌、硼、锰等物质。桑叶中含有蜕皮固酮、羽扁豆酮以及靶香甙、桑甙等许多氨基酸、维生素、绦原酸、叶酸、延胡索酸、内消旋肌酸、植物雌激素等人体所物质,具有抗应激、抗衰老、增强肌体耐力,调节肾上腺素功能等效果。 Mulberry leaves are the dry leaves of the Moraceae plant mulberry, which is the daily food of silkworms; there are various vitamins and minerals, amino acids, carbohydrates and plant fibers in the mulberry leaves. Modern science shows that mulberry leaf is a good functional food. It can lower blood pressure, lower fat, anti-aging, increase endurance, lower cholesterol, inhibit fat accumulation, inhibit thrombosis, inhibit the reproduction of harmful bacteria in the intestine, and inhibit harmful oxides Formation, the most prominent function is to prevent diabetes; According to modern research, dry mulberry leaves contain 25-45% crude protein, 20-25% carbohydrates, 5% crude fat, and rich potassium, calcium and vitamin C, B1, B2, A, etc., as well as various trace elements copper, zinc, boron, manganese and other substances. Mulberry leaves contain ecdysterone, lulenone, and many amino acids such as saponin and morangin, vitamins, citric acid, folic acid, fumaric acid, mesocreatine, phytoestrogens and other human substances, which have anti-stress properties. , Anti-aging, enhance body endurance, regulate adrenaline function and other effects.

桑枝,为果桑的桑枝、桑条、嫩桑枝的总称。在中药学上,桑枝具有祛风通络、利水气、消浮肿、散风热等多种功效,当其与桑叶和桑葚配伍应用后,三者的药用的组合效果更佳。 Sangzhi is the general term for mulberry branches, mulberry sticks and tender mulberry branches of fruit mulberry. In traditional Chinese medicine, mulberry branches have multiple effects such as dispelling wind and dredging collaterals, diuresis, reducing edema, dispelling wind and heat, etc. When it is used in combination with mulberry leaves and mulberries, the medicinal combination of the three will have a better effect.

发明内容 Contents of the invention

针对上述存在的问题,本发明目的在于提供一种生产工艺简单,充分利用果桑上的各个组份,各组分协同功效好,色泽澄清的复合型果酒的生产方法。 In view of the above-mentioned problems, the purpose of the present invention is to provide a production method of compound fruit wine with simple production process, full use of each component on the fruit mulberry, good synergistic effect of each component, and clear color.

为了达到上述目的,本发明采用的技术方案如下:一种利用果桑生产的复合型果酒的方法,所述的复合型果酒由原料桑葚、桑叶和桑枝组合发酵而成,其中原料中桑葚、桑叶和桑枝的用量比为10:1~1.5:0.1~0.3。 In order to achieve the above object, the technical scheme adopted in the present invention is as follows: a method for compound fruit wine produced by using fruit mulberry, wherein the compound fruit wine is fermented from raw materials mulberry, mulberry leaves and mulberry branches, wherein the raw material is mulberry , The dosage ratio of mulberry leaves and mulberry branches is 10:1~1.5:0.1~0.3.

本发明提供的一种利用果桑生产的复合型果酒的方法,包括如下步骤: A kind of method utilizing the compound type fruit wine that fruit mulberry is produced provided by the invention comprises the following steps:

1)选择新鲜成熟的桑葚、颜色翠绿无任何坏点的桑叶、直径不超过的0.3cm的桑枝,在超声波振荡下的清水中清洗10~15min,清洗完成后,沥去水份,0℃下保存待用; 1) Select fresh and mature mulberries, mulberry leaves with emerald green color without any bad spots, and mulberry branches with a diameter of no more than 0.3cm, and wash them in clean water under ultrasonic vibration for 10 to 15 minutes. After cleaning, drain the water, 0 Store at ℃ for later use;

2)称取相应比例的桑葚、桑叶原料以及质量为混合桑葚、桑叶原料总质量1.5~2倍的去离子水,将三者投入到搅拌设备中进行混合打浆,混合时间为20~30min,混合温度不超过50℃; 2) Weigh the corresponding proportion of mulberry and mulberry leaf raw materials and deionized water whose mass is 1.5 to 2 times the total mass of the mixed mulberry and mulberry leaf raw materials, and put the three into the mixing equipment for mixing and beating. The mixing time is 20 to 30 minutes , the mixing temperature does not exceed 50°C;

3)在混合后的浆液中,以的混合浆液体积为1L来算,加入100~120mg/L的白砂糖进行混合,混合完成后,对其进行微波灭菌30~60s; 3) In the mixed slurry, if the volume of the mixed slurry is 1L, add 100-120mg/L white granulated sugar for mixing. After the mixing is completed, microwave it for 30-60s;

4)灭菌完成后,向杀菌后的混合果浆中加入100~350mg/L的复合干酵母,控制在20~30℃的调节下,持续发酵10~15天; 4) After the sterilization is completed, add 100-350mg/L compound dry yeast to the sterilized mixed fruit pulp, and control it at 20-30°C to continue fermentation for 10-15 days;

5)发酵的同时,称取相应比例的桑枝原料,将其投入到粉碎机中进行粉碎处理,粉碎完成后采用筛网过滤,得到颗粒直径不超过0.2cm的桑枝颗粒; 5) At the same time of fermentation, weigh the corresponding proportion of mulberry branch raw materials, put it into the pulverizer for crushing treatment, and use a sieve to filter after the crushing is completed to obtain mulberry branch particles with a particle diameter of no more than 0.2cm;

6)将桑枝颗粒投入到质量为其质量2~2.5倍的去离子水中,加入0.2~0.3g/L的纤维素酶和0.1~0.15g/L的麦芽糖酶进行酶解,酶解时间为2h,酶解完成后,对其进行微波灭菌处理30~60s; 6) Put the mulberry twig granules into deionized water whose mass is 2-2.5 times its mass, add 0.2-0.3g/L cellulase and 0.1-0.15g/L maltase for enzymatic hydrolysis, and the enzymatic hydrolysis time is 2h, after the enzymatic hydrolysis is completed, it is sterilized by microwave for 30-60s;

7)将步骤4)得到的发酵酒液和步骤6)得到的桑枝混合液进行混合,混合完成后,以混合浆液体积为1L来算,补充加入200~220mg/L的白砂糖和200~250mg/L的复合干酵母,在15~20℃的条件下持续发酵30~35天,发酵结束后,得到发酵产物; 7) Mix the fermented wine liquid obtained in step 4) with the mulberry branch mixture obtained in step 6). After the mixing is completed, the volume of the mixed slurry is calculated as 1L, and 200-220mg/L of white sugar and 200- 250mg/L compound dry yeast, continuously fermented at 15-20°C for 30-35 days, after the fermentation, the fermentation product was obtained;

9)在得到的发酵产物中,以的发酵产物的体积为1L来算,加入2.5~3.0g/L的果胶酶,在40~45℃下搅拌40~60min,除去果胶质,然后才有筛网进行过滤,去除发酵产物中的果胶质和其他固体杂质,得到原酒; 9) Add 2.5-3.0g/L pectinase to the fermented product obtained by taking the volume of the fermented product as 1L, and stir at 40-45°C for 40-60min to remove pectin, and then There is a sieve for filtering to remove pectin and other solid impurities in the fermentation product to obtain raw wine;

10)在得到的原酒中,以原酒的体积为1L来算,加入1.2~2.0g/L的壳聚糖和3.0~4.0g/L的蛋清粉,保持温度在30~35℃下,静置24~36h后,采用滤纸进行过滤; 10) Add 1.2-2.0g/L of chitosan and 3.0-4.0g/L of egg white powder to the obtained original wine, taking the volume of the original wine as 1L, keep the temperature at 30-35°C, and let stand After 24-36 hours, use filter paper to filter;

11)将过滤后的酒液在5~10℃下静置12h,静置完成后采用微孔膜进行二次过滤,二次过滤后进行微波灭菌1~2h; 11) Let the filtered liquor stand at 5-10°C for 12 hours. After standing, use a microporous membrane for secondary filtration. After the secondary filtration, perform microwave sterilization for 1-2 hours;

12)将灭菌后的酒液进行灌装和密封操作。 12) Fill and seal the sterilized liquor.

本发明所述的步骤3)、6)、11)步骤中,微波灭菌的同时进行搅拌操作,微波灭菌的微波频率为2200MHz,微波功率15~40kw,处理温度不超过50℃,搅拌速率为60~120r/min。 In steps 3), 6) and 11) of the present invention, the stirring operation is carried out while microwave sterilization is carried out, the microwave frequency of microwave sterilization is 2200MHz, the microwave power is 15-40kw, the processing temperature does not exceed 50°C, and the stirring rate 60~120r/min.

本发明所述的步骤1)中选取的桑叶为去除叶柄后的桑叶,桑叶的长度不低于10cm。本发明的选用的桑叶,既不为老叶,也不为新叶,新叶处于刚生长阶段,其内部的有效成分和营养成本不全面;老叶处于生长末期,其内部的有效成分会分解,供给给其他叶片的生长,因此在两者在选用过程中均需去除。 The mulberry leaves selected in the step 1) of the present invention are the mulberry leaves after the petioles are removed, and the length of the mulberry leaves is not less than 10 cm. The selected mulberry leaves of the present invention are neither old leaves nor new leaves, and the new leaves are in the growth stage, and the active ingredients and nutritional cost inside are not comprehensive; Decompose and feed the growth of other leaves, so both need to be removed during the selection process.

本发明所述的步骤1)中选取的桑枝为截取的果桑枝条端部的桑枝,其截取长度不超过5cm;桑枝选用生长最为旺盛的果桑枝条端部,其内部含有较多的营养元素,而且处理起来方便。 The mulberry branch selected in step 1) of the present invention is the mulberry branch of the intercepted fruit mulberry branch end, and its interception length is no more than 5cm; Nutrients, and easy to handle.

本发明所述的步骤10)的操作过程中,根据最终产品的要求,对原酒糖度和酒精度进行必要的调整;在实际生产过程中,可以根据客户的不同口味需求,在该步骤选择添加白砂糖或者食用酒精,添加的组份不会对原酒中的其他组份发生影响,丰富了产品种类。 In the operation process of step 10) of the present invention, necessary adjustments are made to the sugar content and alcohol content of the original wine according to the requirements of the final product; in the actual production process, white wine can be selected to be added in this step according to the different taste requirements of customers. Sugar or edible alcohol, the added components will not affect other components in the original wine, which enriches the product variety.

本发明的优点在于:本发明选用果桑上的各个组分作为酿果酒的原料,不仅充分利用了果桑药用价值,而且组合连用的桑葚、桑叶和桑枝,发酵产生酒精,还有利于桑叶和桑枝的醇溶性有效成分溶出,发酵完成后,最终产品的中的有效成分:黄酮类、多酚类和花色苷的含量均产生了一定程度的提高;而且在发酵过程中,桑叶和桑枝的颗粒可以为酵母菌的发酵提供附着,有利于原料的充分发酵,提高了发酵的生产效率;本发明的生产方法中采用了分段发酵的原理,主要是因为桑枝本身的营养成分相比桑葚和桑叶来说要少,过长时间的发酵会导致起有效成分发生分解,最终影响三者的协同作用,导致最终产品中的有效成分下降。 The advantage of the present invention is that: the present invention selects each component on fruit mulberry as the raw material of brewing fruit wine, which not only makes full use of the medicinal value of fruit mulberry, but also combines mulberry, mulberry leaves and mulberry branches to produce alcohol through fermentation, and It is conducive to the dissolution of the alcohol-soluble active ingredients of mulberry leaves and mulberry branches. After the fermentation is completed, the contents of active ingredients in the final product: flavonoids, polyphenols and anthocyanins have all increased to a certain extent; and during the fermentation process, The particles of mulberry leaves and mulberry branches can provide attachment for the fermentation of yeast, which is conducive to the full fermentation of raw materials and improves the production efficiency of fermentation; the principle of staged fermentation is adopted in the production method of the present invention, mainly because the mulberry branches themselves Compared with mulberry and mulberry leaves, the nutritional components of mulberry are less, and the long-term fermentation will lead to the decomposition of active ingredients, which will eventually affect the synergy of the three, resulting in a decline in the active ingredients in the final product.

具体实施方式 detailed description

下面结合具体实施方式对本发明作进一步详细的描述。 The present invention will be further described in detail below in combination with specific embodiments.

实施例 1 一种利用果桑生产的复合型果酒的方法,原料中桑葚、桑叶和桑枝的用量比为10:1:0.1,包括如下步骤: Embodiment 1 : a kind of method utilizing the composite fruit wine of fruit mulberry production, the consumption ratio of mulberry, mulberry leaf and mulberry branch is 10:1:0.1 in the raw material, comprises the steps:

1)选择新鲜成熟的桑葚、颜色翠绿无任何坏点的桑叶、直径不超过的0.3cm的桑枝,在超声波振荡下的清水中清洗10~15min,清洗完成后,沥去水份,0℃下保存待用; 1) Select fresh and mature mulberries, mulberry leaves with emerald green color without any bad spots, and mulberry branches with a diameter of no more than 0.3cm, and wash them in clean water under ultrasonic vibration for 10 to 15 minutes. After cleaning, drain the water, 0 Store at ℃ for later use;

2)称取相应比例的桑葚、桑叶原料以及质量为混合桑葚、桑叶原料总质量1.8倍的去离子水,将三者投入到搅拌设备中进行混合打浆,混合时间为20~30min,混合温度不超过50℃; 2) Weigh the corresponding proportion of mulberry and mulberry leaf raw materials and deionized water whose mass is 1.8 times the total mass of mixed mulberry and mulberry leaf raw materials, and put the three into the mixing equipment for mixing and beating. The mixing time is 20-30min, and the mixing The temperature does not exceed 50°C;

3)在混合后的浆液中,以的混合浆液体积为1L来算,加入110mg/L的白砂糖进行混合,混合完成后,对其进行微波灭菌30~60s; 3) In the mixed slurry, if the volume of the mixed slurry is 1L, add 110mg/L white granulated sugar for mixing. After the mixing is completed, microwave it for 30-60s;

4)灭菌完成后,向杀菌后的混合果浆中加入200mg/L的复合干酵母,控制在20~30℃的调节下,持续发酵10~15天; 4) After the sterilization is completed, add 200mg/L compound dry yeast to the sterilized mixed fruit pulp, and control it at 20-30°C, and continue to ferment for 10-15 days;

5)发酵的同时,称取相应比例的桑枝原料,将其投入到粉碎机中进行粉碎处理,粉碎完成后采用筛网过滤,得到颗粒直径不超过0.2cm的桑枝颗粒; 5) At the same time of fermentation, weigh the corresponding proportion of mulberry branch raw materials, put it into the pulverizer for crushing treatment, and use a sieve to filter after the crushing is completed to obtain mulberry branch particles with a particle diameter of no more than 0.2cm;

6)将桑枝颗粒投入到质量为其质量2.2倍的去离子水中,加入0.25g/L的纤维素酶和0.12g/L的麦芽糖酶进行酶解,酶解时间为2h,酶解完成后,对其进行微波灭菌处理30~60s; 6) Put the mulberry twig granules into deionized water whose mass is 2.2 times its mass, add 0.25g/L cellulase and 0.12g/L maltase for enzymolysis, the enzymolysis time is 2h, after the enzymolysis is completed , subject it to microwave sterilization for 30-60s;

7)将步骤4)得到的发酵酒液和步骤6)得到的桑枝混合液进行混合,混合完成后,以混合浆液体积为1L来算,补充加入210mg/L的白砂糖和240mg/L的复合干酵母,在15~20℃的条件下持续发酵30~35天,发酵结束后,得到发酵产物; 7) Mix the fermented wine liquid obtained in step 4) with the mulberry branch mixture obtained in step 6). After the mixing is completed, the volume of the mixed slurry is calculated as 1L, and 210mg/L of white sugar and 240mg/L of Compound dry yeast, continuously fermented at 15-20°C for 30-35 days, after the fermentation, the fermentation product is obtained;

9)在得到的发酵产物中,以的发酵产物的体积为1L来算,加入2.8g/L的果胶酶,在40~45℃下搅拌40~60min,除去果胶质,然后才有筛网进行过滤,去除发酵产物中的果胶质和其他固体杂质,得到原酒; 9) In the fermented product obtained, the volume of the fermented product is 1L, add 2.8g/L pectinase, stir at 40-45°C for 40-60min, remove pectin, and then sieve Filtrate through a net to remove pectin and other solid impurities in the fermentation product to obtain raw wine;

10)在得到的原酒中,以原酒的体积为1L来算,加入1.5g/L的壳聚糖和3.5g/L的蛋清粉,保持温度在30~35℃下,静置24~36h后,采用滤纸进行过滤; 10) Add 1.5g/L chitosan and 3.5g/L egg white powder to the obtained original wine, taking the volume of the original wine as 1L, keep the temperature at 30-35°C, and let it stand for 24-36 hours , using filter paper to filter;

11)将过滤后的酒液在5~10℃下静置12h,静置完成后采用微孔膜进行二次过滤,二次过滤后进行微波灭菌1~2h; 11) Let the filtered liquor stand at 5-10°C for 12 hours. After standing, use a microporous membrane for secondary filtration. After the secondary filtration, perform microwave sterilization for 1-2 hours;

12)将灭菌后的酒液进行灌装和密封操作。 12) Fill and seal the sterilized liquor.

实施例 2 一种利用果桑生产的复合型果酒的方法,原料中桑葚、桑叶和桑枝的用量比为10: 1.5: 0.3,包括如下步骤: Embodiment 2 : a kind of method utilizing the compound fruit wine of fruit mulberry production, the consumption ratio of mulberry, mulberry leaf and mulberry branch is 10: 1.5: 0.3 in the raw material, comprises the steps:

1)选择新鲜成熟的桑葚、颜色翠绿无任何坏点的桑叶、直径不超过的0.3cm的桑枝,在超声波振荡下的清水中清洗10~15min,清洗完成后,沥去水份,0℃下保存待用; 1) Select fresh and mature mulberries, mulberry leaves with emerald green color without any bad spots, and mulberry branches with a diameter of no more than 0.3cm, and wash them in clean water under ultrasonic vibration for 10 to 15 minutes. After cleaning, drain the water, 0 Store at ℃ for later use;

2)称取相应比例的桑葚、桑叶原料以及质量为混合桑葚、桑叶原料总质量1.5倍的去离子水,将三者投入到搅拌设备中进行混合打浆,混合时间为20~30min,混合温度不超过50℃; 2) Weigh the corresponding proportion of mulberry and mulberry leaf raw materials and deionized water whose mass is 1.5 times the total mass of the mixed mulberry and mulberry leaf raw materials, put the three into the mixing equipment for mixing and beating, the mixing time is 20-30min, and the mixing The temperature does not exceed 50°C;

3)在混合后的浆液中,以的混合浆液体积为1L来算,加入100mg/L的白砂糖进行混合,混合完成后,对其进行微波灭菌30~60s; 3) In the mixed slurry, if the volume of the mixed slurry is 1L, add 100mg/L white granulated sugar for mixing. After the mixing is completed, microwave it for 30-60s;

4)灭菌完成后,向杀菌后的混合果浆中加入100mg/L的复合干酵母,控制在20~30℃的调节下,持续发酵10~15天; 4) After the sterilization is completed, add 100mg/L compound dry yeast to the sterilized mixed fruit pulp, and control it at 20-30°C, and continue to ferment for 10-15 days;

5)发酵的同时,称取相应比例的桑枝原料,将其投入到粉碎机中进行粉碎处理,粉碎完成后采用筛网过滤,得到颗粒直径不超过0.2cm的桑枝颗粒; 5) At the same time of fermentation, weigh the corresponding proportion of mulberry branch raw materials, put it into the pulverizer for crushing treatment, and use a sieve to filter after the crushing is completed to obtain mulberry branch particles with a particle diameter of no more than 0.2cm;

6)将桑枝颗粒投入到质量为其质量2倍的去离子水中,加入0.2g/L的纤维素酶和0.1g/L的麦芽糖酶进行酶解,酶解时间为2h,酶解完成后,对其进行微波灭菌处理30~60s; 6) Put the mulberry twig granules into deionized water twice its mass, add 0.2g/L cellulase and 0.1g/L maltase for enzymolysis, the enzymolysis time is 2h, after the enzymolysis is completed , subject it to microwave sterilization for 30-60s;

7)将步骤4)得到的发酵酒液和步骤6)得到的桑枝混合液进行混合,混合完成后,以混合浆液体积为1L来算,补充加入200mg/L的白砂糖和200mg/L的复合干酵母,在15~20℃的条件下持续发酵30~35天,发酵结束后,得到发酵产物; 7) Mix the fermented wine liquid obtained in step 4) with the mulberry branch mixture obtained in step 6). After the mixing is completed, the volume of the mixed slurry is calculated as 1L, and 200mg/L of white sugar and 200mg/L of Compound dry yeast, continuously fermented at 15-20°C for 30-35 days, after the fermentation, the fermentation product is obtained;

9)在得到的发酵产物中,以的发酵产物的体积为1L来算,加入2.5g/L的果胶酶,在40~45℃下搅拌40~60min,除去果胶质,然后才有筛网进行过滤,去除发酵产物中的果胶质和其他固体杂质,得到原酒; 9) In the fermented product obtained, the volume of the fermented product is calculated as 1L, add 2.5g/L pectinase, stir at 40-45°C for 40-60min, remove pectin, and then sieve Filtrate through a net to remove pectin and other solid impurities in the fermentation product to obtain raw wine;

10)在得到的原酒中,以原酒的体积为1L来算,加入1.2g/L的壳聚糖和3.0g/L的蛋清粉,保持温度在30~35℃下,静置24~36h后,采用滤纸进行过滤; 10) Add 1.2g/L chitosan and 3.0g/L egg white powder to the obtained original wine, taking the volume of the original wine as 1L, keep the temperature at 30-35°C, and let it stand for 24-36 hours , using filter paper to filter;

11)将过滤后的酒液在5~10℃下静置12h,静置完成后采用微孔膜进行二次过滤,二次过滤后进行微波灭菌1~2h; 11) Let the filtered liquor stand at 5-10°C for 12 hours. After standing, use a microporous membrane for secondary filtration. After the secondary filtration, perform microwave sterilization for 1-2 hours;

12)将灭菌后的酒液进行灌装和密封操作。 12) Fill and seal the sterilized liquor.

实施例 3 一种利用果桑生产的复合型果酒的方法,原料中桑葚、桑叶和桑枝的用量比为10:1.2:0.2,包括如下步骤: Embodiment 3 : a kind of method utilizing the composite fruit wine of fruit mulberry production, the consumption ratio of mulberry, mulberry leaf and mulberry branch is 10:1.2:0.2 in the raw material, comprises the steps:

1)选择新鲜成熟的桑葚、颜色翠绿无任何坏点的桑叶、直径不超过的0.3cm的桑枝,在超声波振荡下的清水中清洗10~15min,清洗完成后,沥去水份,0℃下保存待用; 1) Select fresh and mature mulberries, mulberry leaves with emerald green color without any bad spots, and mulberry branches with a diameter of no more than 0.3cm, and wash them in clean water under ultrasonic vibration for 10 to 15 minutes. After cleaning, drain the water, 0 Store at ℃ for later use;

2)称取相应比例的桑葚、桑叶原料以及质量为混合桑葚、桑叶原料总质量2倍的去离子水,将三者投入到搅拌设备中进行混合打浆,混合时间为20~30min,混合温度不超过50℃; 2) Weigh the corresponding proportion of mulberry and mulberry leaf raw materials and deionized water whose mass is twice the total mass of the mixed mulberry and mulberry leaf raw materials, put the three into the mixing equipment for mixing and beating, the mixing time is 20-30min, and The temperature does not exceed 50°C;

3)在混合后的浆液中,以的混合浆液体积为1L来算,加入120mg/L的白砂糖进行混合,混合完成后,对其进行微波灭菌30~60s; 3) In the mixed slurry, the volume of the mixed slurry is 1L, add 120mg/L white sugar for mixing, after the mixing is completed, it is microwave sterilized for 30-60s;

4)灭菌完成后,向杀菌后的混合果浆中加入350mg/L的复合干酵母,控制在20~30℃的调节下,持续发酵10~15天; 4) After the sterilization is completed, add 350mg/L compound dry yeast to the sterilized mixed fruit pulp, and control it at 20-30°C, and continue to ferment for 10-15 days;

5)发酵的同时,称取相应比例的桑枝原料,将其投入到粉碎机中进行粉碎处理,粉碎完成后采用筛网过滤,得到颗粒直径不超过0.2cm的桑枝颗粒; 5) At the same time of fermentation, weigh the corresponding proportion of mulberry branch raw materials, put it into the pulverizer for crushing treatment, and use a sieve to filter after the crushing is completed to obtain mulberry branch particles with a particle diameter of no more than 0.2cm;

6)将桑枝颗粒投入到质量为其质量2.5倍的去离子水中,加入0.3g/L的纤维素酶和0.15g/L的麦芽糖酶进行酶解,酶解时间为2h,酶解完成后,对其进行微波灭菌处理30~60s; 6) Put the mulberry twig granules into deionized water whose mass is 2.5 times its mass, add 0.3g/L cellulase and 0.15g/L maltase for enzymolysis, the enzymolysis time is 2h, after the enzymolysis is completed , subject it to microwave sterilization for 30-60s;

7)将步骤4)得到的发酵酒液和步骤6)得到的桑枝混合液进行混合,混合完成后,以混合浆液体积为1L来算,补充加入220mg/L的白砂糖和250mg/L的复合干酵母,在15~20℃的条件下持续发酵30~35天,发酵结束后,得到发酵产物; 7) Mix the fermented wine liquid obtained in step 4) with the mulberry branch mixture obtained in step 6). After the mixing is completed, the volume of the mixed slurry is calculated as 1L, and 220mg/L of white sugar and 250mg/L of Compound dry yeast, continuously fermented at 15-20°C for 30-35 days, after the fermentation, the fermentation product is obtained;

9)在得到的发酵产物中,以的发酵产物的体积为1L来算,加入3.0g/L的果胶酶,在45℃下搅拌40~60min,除去果胶质,然后才有筛网进行过滤,去除发酵产物中的果胶质和其他固体杂质,得到原酒; 9) In the fermented product obtained, the volume of the fermented product is calculated as 1L, add 3.0g/L pectinase, stir at 45°C for 40-60min, remove the pectin, and then carry out the sieve Filtration to remove pectin and other solid impurities in the fermentation product to obtain raw wine;

10)在得到的原酒中,以原酒的体积为1L来算,加入2.0g/L的壳聚糖和4.0g/L的蛋清粉,保持温度在30~35℃下,静置24~36h后,采用滤纸进行过滤; 10) Add 2.0g/L chitosan and 4.0g/L egg white powder to the obtained original wine, taking the volume of the original wine as 1L, keep the temperature at 30-35°C, and let it stand for 24-36 hours , using filter paper to filter;

11)将过滤后的酒液在5~10℃下静置12h,静置完成后采用微孔膜进行二次过滤,二次过滤后进行微波灭菌1~2h; 11) Let the filtered liquor stand at 5-10°C for 12 hours. After standing, use a microporous membrane for secondary filtration. After the secondary filtration, perform microwave sterilization for 1-2 hours;

12)将灭菌后的酒液进行灌装和密封操作。 12) Fill and seal the sterilized liquor.

实施例 4 在其他原料组份和生产工艺条件不变的情况下,对本发明的生产配方进行对比试验,最终得到的结果如下表所示: Embodiment 4 : under the constant situation of other raw material components and production process condition, comparative test is carried out to production formula of the present invention, the result finally obtained is shown in the following table:

由上表可知:本发明的生产方法中,当桑葚:桑叶:桑枝三者进行联用时,最终得到的产品果酒中的有效成分得到了提高,当缺少其中任意组分后,其果酒中的有效成分发生明显的降低;但是三者联用的比例存在一定的限制,不能过量,当桑叶或者桑枝的用量过多后,最终果酒在长时间低于常温的条件下存放后会产生少量絮凝,桑叶和桑枝中的其他发酵后的组分在长时间低温条件下存放后,会发生一定程度上的改变,影响果酒的存放和销售。 It can be known from the above table: in the production method of the present invention, when mulberry: mulberry leaves: when the three of mulberry branches are used in combination, the active ingredients in the product fruit wine finally obtained are improved. The effective components of the three components are significantly reduced; however, there is a certain limit to the ratio of the combination of the three, and it cannot be excessive. When the amount of mulberry leaves or mulberry branches is too much, the final fruit wine will be produced after being stored for a long time under the condition of lower than normal temperature. A small amount of flocculation, and other fermented components in mulberry leaves and mulberry branches will change to a certain extent after long-term storage at low temperature, which will affect the storage and sales of fruit wine.

实施例 5 在其他原料组份和生产工艺条件不变的情况下,对本发明的生产工艺进行对比试验,最终得到的结果如下表所示: Embodiment 5 : under the constant situation of other raw material components and production technology condition, comparative test is carried out to production technology of the present invention, the result that finally obtains is shown in the following table:

由上表可知:本发明的生产过程中,需要首先将桑葚和桑叶同时进行发酵,发酵生产一段时间后,再将桑枝投入到发酵液中进行发酵,避免了在实际生产过程中,桑枝因过长时间发酵导致最终产品中有效成分含量下降的问题,本发明的工艺针对生产过程中遭遇的实际问题,改进原有的生产工艺,最终达到生产的果酒中有效成分含量得到提升,优化了整体生产的生产工艺。 As can be seen from the above table: in the production process of the present invention, at first mulberry and mulberry leaves need to be fermented simultaneously, and after a period of time of fermentation production, the mulberry branches are put into the fermented liquid for fermentation, so as to avoid the mulberry leaves in the actual production process. Due to the problem that the content of active ingredients in the final product decreases due to prolonged fermentation, the process of the present invention aims at the actual problems encountered in the production process, improves the original production process, and finally achieves that the content of active ingredients in the produced fruit wine is improved and optimized. The production process of the overall production.

需要说明的是,上述仅仅是本发明的较佳实施例,并非用来限定本发明的保护范围,在上述实施例的基础上所做出的任意组合或等同变换均属于本发明的保护范围。 It should be noted that the above are only preferred embodiments of the present invention, and are not intended to limit the protection scope of the present invention. Any combination or equivalent transformation made on the basis of the above embodiments belongs to the protection scope of the present invention.

Claims (5)

1.一种利用果桑生产的复合型果酒的方法,其特征在于,所述的复合型果酒由原料桑葚、桑叶和桑枝组合发酵而成,其中原料中桑葚、桑叶和桑枝的用量比为10:1~1.5:0.1~0.3;所述的生产方法包括如下步骤: 1. a method for the compound fruit wine utilizing fruit mulberry to produce, it is characterized in that, described compound fruit wine is formed by the combination fermentation of raw material mulberry, mulberry leaf and mulberry branch, wherein in the raw material mulberry, mulberry leaf and mulberry branch The dosage ratio is 10:1~1.5:0.1~0.3; the described production method comprises the following steps: 1)选择新鲜成熟的桑葚、颜色翠绿无任何坏点的桑叶、直径不超过的0.3cm的桑枝,在超声波振荡下的清水中清洗10~15min,清洗完成后,沥去水份,0℃下保存待用; 1) Select fresh and mature mulberries, mulberry leaves with emerald green color without any bad spots, and mulberry branches with a diameter of no more than 0.3cm, and wash them in clean water under ultrasonic vibration for 10 to 15 minutes. After cleaning, drain the water, 0 Store at ℃ for later use; 2)称取相应比例的桑葚、桑叶原料以及质量为混合桑葚、桑叶原料总质量1.5~2倍的去离子水,将三者投入到搅拌设备中进行混合打浆,混合时间为20~30min,混合温度不超过50℃; 2) Weigh the corresponding proportion of mulberry and mulberry leaf raw materials and deionized water whose mass is 1.5 to 2 times the total mass of the mixed mulberry and mulberry leaf raw materials, and put the three into the mixing equipment for mixing and beating. The mixing time is 20 to 30 minutes , the mixing temperature does not exceed 50°C; 3)在混合后的浆液中,以的混合浆液体积为1L来算,加入100~120mg/L的白砂糖进行混合,混合完成后,对其进行微波灭菌30~60s; 3) In the mixed slurry, if the volume of the mixed slurry is 1L, add 100-120mg/L white granulated sugar for mixing. After the mixing is completed, microwave it for 30-60s; 4)灭菌完成后,向杀菌后的混合果浆中加入100~350mg/L的复合干酵母,控制在20~30℃的调节下,持续发酵10~15天; 4) After the sterilization is completed, add 100-350mg/L compound dry yeast to the sterilized mixed fruit pulp, and control it at 20-30°C to continue fermentation for 10-15 days; 5)发酵的同时,称取相应比例的桑枝原料,将其投入到粉碎机中进行粉碎处理,粉碎完成后采用筛网过滤,得到颗粒直径不超过0.2cm的桑枝颗粒; 5) At the same time of fermentation, weigh the corresponding proportion of mulberry branch raw materials, put it into the pulverizer for crushing treatment, and use a sieve to filter after the crushing is completed to obtain mulberry branch particles with a particle diameter of no more than 0.2cm; 6)将桑枝颗粒投入到质量为其质量2~2.5倍的去离子水中,加入0.2~0.3g/L的纤维素酶和0.1~0.15g/L的麦芽糖酶进行酶解,酶解时间为2h,酶解完成后,对其进行微波灭菌处理30~60s; 6) Put the mulberry twig granules into deionized water whose mass is 2-2.5 times its mass, add 0.2-0.3g/L cellulase and 0.1-0.15g/L maltase for enzymatic hydrolysis, and the enzymatic hydrolysis time is 2h, after the enzymatic hydrolysis is completed, it is sterilized by microwave for 30-60s; 7)将步骤4)得到的发酵酒液和步骤6)得到的桑枝混合液进行混合,混合完成后,以混合浆液体积为1L来算,补充加入200~220mg/L的白砂糖和200~250mg/L的复合干酵母,在15~20℃的条件下持续发酵30~35天,发酵结束后,得到发酵产物; 7) Mix the fermented wine liquid obtained in step 4) with the mulberry branch mixture obtained in step 6). After the mixing is completed, the volume of the mixed slurry is calculated as 1L, and 200-220mg/L of white sugar and 200- 250mg/L compound dry yeast, continuously fermented at 15-20°C for 30-35 days, after the fermentation, the fermentation product was obtained; 9)在得到的发酵产物中,以的发酵产物的体积为1L来算,加入2.5~3.0g/L的果胶酶,在40~45℃下搅拌40~60min,除去果胶质,然后才有筛网进行过滤,去除发酵产物中的果胶质和其他固体杂质,得到原酒; 9) Add 2.5-3.0g/L pectinase to the fermented product obtained by taking the volume of the fermented product as 1L, and stir at 40-45°C for 40-60min to remove pectin, and then There is a sieve for filtering to remove pectin and other solid impurities in the fermentation product to obtain raw wine; 10)在得到的原酒中,以原酒的体积为1L来算,加入1.2~2.0g/L的壳聚糖和3.0~4.0g/L的蛋清粉,保持温度在30~35℃下,静置24~36h后,采用滤纸进行过滤; 10) Add 1.2-2.0g/L of chitosan and 3.0-4.0g/L of egg white powder to the obtained original wine, taking the volume of the original wine as 1L, keep the temperature at 30-35°C, and let stand After 24-36 hours, use filter paper to filter; 11)将过滤后的酒液在5~10℃下静置12h,静置完成后采用微孔膜进行二次过滤,二次过滤后进行微波灭菌1~2h; 11) Let the filtered liquor stand at 5-10°C for 12 hours. After standing, use a microporous membrane for secondary filtration. After the secondary filtration, perform microwave sterilization for 1-2 hours; 12)将灭菌后的酒液进行灌装和密封操作。 12) Fill and seal the sterilized liquor. 2.根据权利要求1所述的利用果桑生产的复合型果酒的方法,其特征在于,所述的步骤3)、6)、11)步骤中,微波灭菌的同时进行搅拌操作,微波灭菌的微波频率为2200MHz,微波功率15~40kw,处理温度不超过50℃,搅拌速率为60~120r/min。 2. The method for compound fruit wine produced by fruit mulberry according to claim 1, characterized in that, in the steps 3), 6) and 11), microwave sterilization is carried out while stirring operation, microwave sterilization The microwave frequency of the bacteria is 2200MHz, the microwave power is 15-40kw, the treatment temperature does not exceed 50°C, and the stirring rate is 60-120r/min. 3.根据权利要求1所述的利用果桑生产的复合型果酒的方法,其特征在于,所述的步骤1)中选取的桑叶为去除叶柄后的桑叶,桑叶的长度不低于10cm。 3. The method for compound fruit wine produced by fruit mulberry according to claim 1, characterized in that, the mulberry leaf selected in the step 1) is the mulberry leaf after the petiole is removed, and the length of the mulberry leaf is not less than 10cm. 4.根据权利要求1所述的利用果桑生产的复合型果酒的方法,其特征在于,所述的步骤1)中选取的桑枝为截取的果桑枝条端部的桑枝,其截取长度不超过5cm。 4. the method for the composite fruit wine that utilizes fruit mulberry to produce according to claim 1, is characterized in that, the mulberry branch that chooses in described step 1) is the mulberry branch of the intercepted fruit mulberry branch end, and its interception length No more than 5cm. 5.根据权利要求1所述的利用果桑生产的复合型果酒的方法,其特征在于,所述的步骤10)的操作过程中,根据最终产品的要求,对原酒糖度和酒精度进行必要的调整。 5. The method for compound fruit wine produced by fruit mulberry according to claim 1, characterized in that, in the operation process of the step 10), according to the requirements of the final product, the sugar content and alcohol content of the raw wine are adjusted as necessary Adjustment.
CN201610575427.3A 2016-07-21 2016-07-21 Method for producing composite fruit wine from mulberries Pending CN105969593A (en)

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