CN1059631A

CN1059631A - Salt algae cultural method and breed pond

Info

Publication number: CN1059631A
Application number: CN91105062A
Authority: CN
Inventors: 滕景琪; 夏树海; 付昌喜; 孙五一
Original assignee: Tanggu General Co Tianjin Real Estate Development & Mangement Croup
Current assignee: Tanggu General Co Tianjin Real Estate Development & Mangement Croup
Priority date: 1991-07-29
Filing date: 1991-07-29
Publication date: 1992-03-25

Abstract

A kind of salt algae cultural method and breed pond.Belong to algae outdoor breeding method, the present invention adopts bittern that bittern or landlocked lake salt be mixed with or intercrystalline bittern, subsurface brine etc. as the salt algae culturing liquid, sterile-processed in culturing the pond by the ratio access algae kind of algae seedling solution and culture fluid 1: 1～20, add a certain amount of nutrient solution, daytime, stirring dynamic was cultured.Treat that the salt algae contains carotin and reaches 9～15g/m ³Shi Caishou adds impervious material to culturing at the bottom of the pond is improved to the pond, and pond peripheral hardware one can hold adding a cover of whole culture fluids and protect kind of a well.

The present invention overcome existing salt algae culture in existing problems, the outdoor algal culture when being applicable to bad weather such as coastal and landlocked product salt area and rain, wind, hail.

Description

Salt algae cultural method and breed pond

The invention belongs to algae outdoor breeding method and culture the pond improvement.

General outdoor breeding method is to insert the salt algae in culturing the pond, and carries out mariculture under field conditions (factors), adds the nutrient solution of different formulations in breeding process, and comparatively Chang Yong nutrient solution prescription is Na ⁺, K ⁺, Ca ⁺⁺, Mg ⁺⁺, Cl ^-, HCO ^- ₃, SO = ₄, Br ^-, Fe ⁺⁺⁺, trace elements such as the interpolation boron that also has, manganese, zinc, molybdenum are as Au65331/86, J ₅₆68388, J ₅₇47476, J ₅₉192083, J ₆₂239981, patent such as Us4350294, Us4199895 all has detailed report to nutrient solution composition and content, wherein Us4199895 has spoken of when culturing the salt algae, must add nutrient component, and control temperature and culture fluid pH value, when being culture medium with the seawater, its nutrient solution consists of: NaCl 1～4Mol; NH ₄NO ₃0.5～2.5mMol; KH ₂PO ₄0.01～1mMol;

FeCl ₃-EDTA 0.5～50mMol; NaHCo ₃10mMol or CO ₂300 l/m ³, CaCo ₃And organic sewage also can be used as carbon source, control culture fluid PH7.0～9.0,25～35 ℃ of temperature.

CN1044200A(90100380.8) narrate nutrient solution and consisted of urea 1-4mMol, KH ₂PO ₄0.1-0.3mMol, NaHCO ₃5-10mMol, FeCl ₃3-6mMol, control PH7-8.

More than each patent report illustrated to be that nutrient component that salt algae culture medium matter is added various components can more effectively be carried out the salt algae and cultivated with the seawater.

Culturing the pond also has multi-form, for example build up booth or in pipe-line system, carry out the sealing chamber breed with transparent material, these two kinds of methods all have its merits and demerits, adopted open-air open breed in recent years, though the method illumination abundance, but the frond growth is subjected to effect of natural conditions bigger, rainfall or evaporation can change condition of culture, these all influence the accumulation of carotin in the continuous growth of frond and the frond, and the erosion of culture fluid infiltration and soil increases the culture fluid turbidity, influence the breeding of algae, for preventing above-mentioned shortcoming, carried out various improvement again to culturing the pond, one deck cement is broken or tiling look seam on cement by U.S. microorganism company at the bottom of culturing the pond, western australia biotech firm also adopts the method for pond base pressure reality, and cement is easily efflorescence in bittern.Service life is short, compacting, the assorted algae of bacterium still easily mixes in culture fluid in the soil, cement upper berth tile pointing, the cost height, CN1044200A adopts polyvinyl chloride, polyethylene film or rubber shop fixtures, the block weight of row pressure certain geometrical shape equably, prevent to pollute at the bottom of the pond, the pond face has added a cover by floating spool, electromotor, track, pulley, the rainproof topped device that film is formed is though preferably resolve the anti-rainproof problem that blends, but the size of film must be bonded by pool size, lay trouble, film is fragile in the time of at the bottom of the cleaning pond, also easily brings assorted bacterium during laying into, especially topped device, long term exposure in the sun, plastic foil is easily aging, with contact with sea water, track, equipment easy-to-rusts such as pulley, maintenance and maintenance capacity are big, and the present invention provides a kind of cultural method applied widely and pond structure for overcoming above-mentioned shortcoming.

The cultural method that reaches of the present invention is: 10～20 ° of Be ' bittern are squeezed in the sterilization pool as culture fluid, the liquor natrii hypochloritis or the Eusol that will contain available chlorine 9～12% add in the bittern with the content that accounts for bittern volume 4/10000ths, after about 24 hours, is the bittern of sterilizing that culture fluid is squeezed into the breed pond, open agitator, culture fluid flows, the algae kind is inserted the breed pond, algae seedling solution is 1: 1～20 with the ratio of culture fluid, the culture fluid depth of water 5～20cm, the algae kind density after the access is no less than 100,000 cells/ml, then by the NPFeC order once or divide 2～8 times to add enough amounts, dosage is urea 1～2mMol, KH ₂PO ₄0.1-0.3mMol, Fecl ₃0.1-0.3mMol, NaHoo ₃5-10mMol, open agitator daytime in the incubation, and stop night, cultivated 7-10 days, treats that carotene carotene content is 9-15g/m in the culture fluid ³In time, can gather, and the culture fluid after gathering can return sterilization pool according to circumstances or culture the pond and continue to use.

Raw brine of the present invention can be the bittern in the salt-making process, or sea, lake, well, rock salt add the bittern that fresh water or bitter are mixed with, and the intercrystalline bittern in the inland brine lake, subsurface brine are formulated.

Thimerosal liquor natrii hypochloritis of the present invention not having source, chlor-alkali plant area at need, can adopt the hypochlorite generator to make by oneself with salt solution, also available bleaching powder Ca(ClO ₂) ₂, or the ozonization of ozone generator generation.

Culture fluid of the present invention, N source can adopt urea, nitrate, P source can use NaH ₂PO ₄, KH ₂PO ₄, Na ₂HPO ₄, K ₂HPO ₄Deng soluble phosphate, Fecl is adopted in the Fe source ₃, Fe ₂(SO ₄) ₃, the C source adopts NaHCO ₃Or sugar.

Breed pond involved in the present invention, adopt angle, rectangle garden, one word or S shape shelves waterwall are arranged in the pond, one end has horizontal stirring water wheels, at the bottom of the pond, make a call to 1～2cm cement layer, coated 0.2～0.5cm waterglass on cement layer, or play the thick magnesia mortar of 1～2cm of folder fiber web frame in one deck or light calcined magnesia mortar to prevent seepage and soil pollution.What an end dug that a underground type or semi-underground type can hold in the pond square, the rectangle of whole culture fluids or garden shape outside the pond adds a cover guarantor's kind well through what antiseepage was handled, according to weather forecast, preceding culture fluid is pumped into of heavy rain added a cover guarantor's kind of well, returns behind the rain again and cultures the pond.

Described magnesia mortar or light calcined magnesia mortar are beaten one deck earlier when construction, spread one deck fibrous material then, beat one deck again, and gross thickness is 1～2cm, and is flexible at the bottom of the pond of handling like this, is difficult for during cleaning trampling at the bottom of the pond.The described protection well borehole wall is built jointing by laying bricks or stones by stone, and handle with quadrat method at the bottom of with the pond in the shaft bottom, and existing so very strong supporting role again can antiseepage.

Cultural method of the present invention is outdoor open dynamic breeding method, goes through evil as culture fluid evaporation and can add fresh water and keep the culture fluid degree of depth, because raw material sources are extensive, thus applied widely, as long as produce the salt area,, coastal or interior ground cultures no matter all can carrying out the salt algae.Pond of the present invention treatment of bottom cost is low, and long service life adopts and adds a cover the protection well, and method is easy, is applicable to various bad weathers such as heavy rain, strong wind, hail, more helps the salt algae and extensively cultures.

Embodiment 1:

With aforesaid through NaClO ₂Or Ca(Clo ₂) ₂Bittern after the sterilization pumps into cultures in the pond as culture fluid, and inserts the algae kind from the inoculation pond to culturing the pond, and algae seedling solution and culture fluid volume ratio are 1: 1, disposable then adding urea 2mMol, KH ₂PO ₄0.3mMol, FeCl ₃0.3mMol, NaHCO ₃10mMol is as nutrient solution, and daytime, whole day stirred, and dynamically cultures can make the salt algae contain carotin after seven days to reach 13g/m ³, then described salt algae is moved to the pond of gathering.

Embodiment 2:

Press embodiment 1 described condition, nutrient solution divided in 7 days to be added for three times, can make described salt algae contain carotin and reach 15g/m ³, change the operation of gathering then over to.

Embodiment 3:

Press the embodiment 1 described algae kind that inserts in culturing the pond, algae seedling solution and culture fluid volume ratio are 1: 5, disposable then adding urea 1.5mMol, KH ₂PO ₄0.2mMol, FeCl ₃0.2mMol, NaHCO ₃8mMol is as nutrient solution, and daytime, stirring dynamic was cultured 8 days, can make described salt algae contain carotin and reach 11g/m ³, gather then.

Embodiment 4:

Press embodiment 3 described conditions, nutrient solution divided secondary to add in 8 days, can make described salt algae contain carotin and reach 12g/m ³, gather then.

Embodiment 5:

Be pressed into the salt algae by embodiment 1 described condition, making algae seedling solution and culture fluid volume ratio is 1: 20, adds urea 1mMol then, KH ₂PO ₄0.1mMol, Fecl ₃0.1mMol, NaHCO ₃5mMol is as nutrient solution, and daytime, stirring dynamic was cultured 10 days, can make the salt algae contain carotin and reach 9g/m ³, change the operation of gathering then over to.

Claims

1, a kind of salt algae cultural method and breed pond, described method is to pump into after seawater is sterilized in sterilization pool to culture in the pond, from the inoculation pond, in described breed pond, insert the algae kind, and add nutrient component such as N.P.Fe.C, stirring dynamic was cultured and can be gathered in 7～10 days daytime, and the present invention is characterised in that culture fluid adopts the bittern of 10～20 ° of Be '; The algae seedling solution in access breed pond is 1:1～20 with the ratio of bittern culture fluid volume; Nutrient solution once adds or divides 2～3 addings; Carotene carotene content reaches 9～15g/m in the salt algae ³The time change the operation of gathering over to.

2, according to claim 1 described salt algae cultural method and breed pond, it is characterized in that described culture fluid bittern can be bittern in the salt-making process, or sea, tide, well, rock salt adds fresh water or bitter is mixed with bittern, or the intercrystalline bittern in the inland brine lake, and subsurface brine is mixed with bittern.

3, a kind of salt algae cultural method and breed pond, described breed pond is to lay impervious material and add rainproof facility in the pond at the bottom of the pond, the present invention is that 1～2cm cement layer adds 0.2～0.5cm waterglass layer at the bottom of being characterised in that described pond, or interior folder fiber web frame thickness is 1～2cm magnesia mortar or light calcined magnesia mortar; Described rainproof facility is that an end is established one and can be held the underground type of handling through antiseepage of whole culture fluids or semi-underground type and add a cover and protect kind of a well outside the pond.