CN106258920A - A kind of algae outdoor propagation method - Google Patents

A kind of algae outdoor propagation method Download PDF

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Publication number
CN106258920A
CN106258920A CN201510321574.3A CN201510321574A CN106258920A CN 106258920 A CN106258920 A CN 106258920A CN 201510321574 A CN201510321574 A CN 201510321574A CN 106258920 A CN106258920 A CN 106258920A
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China
Prior art keywords
culturing pool
pond
salt alga
algae
cultivation
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CN201510321574.3A
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Chinese (zh)
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胡培芹
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Individual
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Individual
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Priority to CN201510321574.3A priority Critical patent/CN106258920A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cultivation Of Seaweed (AREA)

Abstract

A kind of salt alga outdoor breeding method and culturing pool, belong to the outdoor propagation method of a kind of algae.Method of the present invention is: accesses described salt alga in culturing pool, and adds a certain amount of nutritional solution in culturing liquid.Cultivate under field conditions (factors).Gather when described salt alga reaches certain density.Improvement to culturing pool refers to install a set of rainproof cladding system being mainly made up of floating spool, motor, track, pulley, coverlay on face, pond.The weight with certain geometrical shape is uniformly arranged at the film shaped material being covered with at the bottom of pond.Instant invention overcomes problem present in the cultivation of existing salt alga, so that being possibly realized in area of heavy rainfull cultivation algae.

Description

A kind of algae outdoor propagation method
Technical field
The invention belongs to a kind of algae outdoor propagation method.
Background technology
Before the present invention realizes, general salt alga outdoor breeding method is to access described salt alga in culturing pool, and carries out sea-farming under field conditions (factors), when density reaches a timing, gathers.And culturing pool is taken various forms.A kind of is to use to build up booth or the closed culture carried out in tubing with transparent material, and this kind of method, to keeping the growth temperature of frond, reduces the evaporation of culture fluid and prevent the pollution of the environment and all have preferable effect.But also make frond light intensity in growth course not enough simultaneously, affect growth and the breeding of frond, and invest bigger.Another kind is the open cultivation of open-air, although the method can illumination sufficient, but the growth easily making frond is affected relatively big by natural conditions, and rainfall or evaporation can make condition of culture change simultaneously, the accumulation of carotin in these growths continuously all affecting frond and frond.More than and, present in two kinds of methods, same problem is exactly
Use which kind of mode all can produce the infiltration of culture fluid and owing to the erosion of soil causes culture fluid turbidity to increase, affect the breeding of algae.
For solving above-mentioned culture fluid seepage and the problem of turbidity increase, now with method be to use certain film shaped material such as plastic sheeting or elastomeric material, non-film shaped material such as potter's clay, ceramics mortar can also be used and spray filmogen shop fixtures in culture pond, so that it is more smooth at the bottom of pond, there is again certain tenacity, antiseepage, it is simple at the bottom of flushing tank.When using film shaped material, generally prevent it from floating in pond and covering a thin sheet of sand or soil in film overlying, but owing to the effect of internal force or external force is easy to make soil or sand float, make culture fluid turbidity increase, make described thin film be more easily damaged, change thin film and make cost increase.Use non-film shaped material the most relatively costly.
Summary of the invention
It is an object of the invention to provide a kind of salt alga outdoor breeding method, and culturing pool is improved, thus overcome problem present in prior art.
Cultural method involved in the present invention is: first by NaClO3Content (accounting for the ratio of sea area for aquatic breeding water) with 4/10000ths adds to the sea water through sedimentation sterilization and pumps in culturing pool after 24 hours.Being accessed by salt alga in culturing pool and making density is 100,000 cells/ml, carries out the cultivation of natural conditions, can also add with urea element 1-4mMol, KH in culturing pool2PO40.1-0.3mMol、NaHCO3: 5-10mMol, FeCL3: 3-6mMol is the nutritional solution of main body, so that frond obtains enough nutrients in growth course.
Urea element in described nutritional solution can with nitrate, ammonium salt with etc. nitrogen content replace.Described FeCL3Can with Organic Iron such as: ferric citrate with etc. iron-holder replace.
Can stir 2 times every day with agitator when cultivation, in culture pond pond, be passed through CO simultaneously2Gas, regulates culture fluid PH7-8.
7-10 days density of salt alga cultivation moves into pond of gathering when reaching 40-60 ten thousand cells/ml and gathers in the above conditions, and the culturing liquid after gathering can be recycled to continue in culturing pool reuse.
Improvement to culturing pool refers to be covered with one layer of film shaped material bottom culturing pool, and arranges the block weight with geometry equably at described shop fixtures material surface.Described shop fixtures material can be polyvinyl chloride plastic film, it is also possible to be rubber, preferably sealed polyethylene plastic.Described block weight is preferably Rhizoma Begoniae Willsonii for building, it is also possible to be cement brick and other density weight more than culture fluid density.When laying, then described film shaped material surface can be arranged in equably with described block weight by described film shape shop fixtures material-paving at the bottom of pond.The most both reach the effect of antiseepage, eliminate again problem present in traditional shop fixtures method.Meanwhile, the gap between evenly distributed block weight promotes that current stir, it is easy to the suspension of frond, beneficially photosynthesis, but also substantially prolongs the service life of described shop fixtures material.
Improving another of described culturing pool refers to as preventing the culturing liquid change caused in described culturing pool because raining, frond is made to grow the most continuously, the face, pond of described culturing pool is mounted with a set of mainly by floating spool, motor, slideway, pulley and face, the pond cladding system of coverlay composition.
Face, described pond cladding system is the steel hollow axle sealed by floating spool, floatability is on face, pond, its axis is meshed with the output shaft of motor by shaft joint, described coverlay is wrapped on the surface of floating spool equably, when carrying out face, pond and covering, described coverlay can swim on the water surface equably.There are some guide wheels, described guide wheel to may be stuck on side, pond two on the guide rail on edge in two sides of described overlay film.The end of described coverlay is connected in essence kickboard.The guide wheel being respectively arranged with in two sides of described kickboard is stuck on side, pond two on the guide rail on edge so that described kickboard drive when motion described in coverlay run along before and after guide rail.Thereby may be ensured that described overlay film can cover on the face, pond of described culturing pool equably or reclaim.The other end of described shaft joint is wound with one end and is connected with the pull rope of a traction car.Two pull ropes that the described tractor other end connects, are connected to described kickboard front end by guide wheel.When needing covering cell face, actuating motor then can be driven tractor to move by shaft joint outfan by pull rope, simultaneously by the guiding of guide wheel, pulls the kickboard of coverlay front end to drive coverlay along guide rail as covering campaign.Meanwhile floating spool is driven to rotate by another outfan of described motor shaft joint so that it is the coverlay of upper winding is constantly released along the water surface, covering cell face.When on the film in covering cell face long-pending have fresh water time, hydrops can be discharged by the light mouth of row of pool side.
When need to reclaim coverlay, only need to make contrary action by procedure above.
Culturing pool involved in the present invention could be applicable to the freshwater aquiculture of the algae such as spirulina sea farming, Xi Zao, spirulina, chlorella.
When the present invention makes rainfall, the cultivation continuously of algae is achieved, and also makes to be possibly realized in area of heavy rainfull cultivation algae.And overcome problem present in existing algal culture.Make algae can obtain enough illumination and nutrient in growth course, and reduce the cost of algal culture.
Embodiment 1:
By aforesaid through NaClO3Sea water after process pumps in culturing pool, and accesses salt alga to culturing pool from inoculation pond, makes the density 100,000 cells/ml of salt alga in culturing pool, is subsequently adding urea element 1mMol, KH2PO4: 0.3mMol, NaHCO3: 8mMol, FeCl3: 3mMol, as nutritional solution, stirs secondary, and is passed through CO in every day2Making culturing liquid PH=7-8 under conditions of gas, cultivate and described salt alga density within 7 days, can be made to reach 400,000 cells/ml, gathers in the pond that then together moves to described salt alga together with culturing liquid to gather.
Embodiment 2
By embodiment 1, add with urea element 4m Mol, KH after accessing salt alga in culturing pool2PO4: 0.2mMol, NaHCO3: 10mMol, FeCl3: 6mMol is main nutritional solution, and cultivation can make described salt alga density reach 600,000 cells/ml for 10 days under the conditions described in example 1, then carries out gathering described in embodiment 1.
Embodiment 3
Urea element 3mMol, KH is added after accessing salt alga in culturing liquid by embodiment 12PO3: 0.3mMol, NaHCO3: 5mMol, FeCl3: 4Mmol is as nutritional solution, and cultivation 8 days under the conditions described in example 1, described salt alga density can be made to reach 500,000 cells/ml, then carry out gathering described in embodiment 1.

Claims (6)

1. a salt alga outdoor breeding method and culturing pool.
2. the method described in is, pump in culturing pool after sea water is sterilized 24 hours with NaClO3 in sterilization pool, in described culturing pool, salt alga is accessed from inoculation pond, and to make the salt alga density in described cultivation be 100,000 cells/ml, cultivate 7-10 days, gather when described salt alga density reaches 40-60 ten thousand cells/ml, it is characterised in that the sea water in described culturing pool can add with urea element 1-4mMol, KH2PO4∶0.1-0.3mMol、NaHCO3、5-10mMol、FeCl3: 3-6mMol is the nutritional solution of main body.
Method the most according to claim 1, it is characterised in that the urea element in described nutritional solution can with nitrate, ammonium salt with etc. nitrogen content replace.
Method the most according to claim 1, it is characterised in that FeCl in described nutritional solution3Can with Organic Iron such as ferric citrate with etc. iron-holder replace.
5. a salt alga outdoor breeding pond, described culturing pool is to be covered with a tunic shape shop fixtures material at the bottom of the pond of the culturing pool of natural or manual manufacture, it is characterized in that, being uniformly arranged with of described shop fixtures material, there is the block weight of certain geometrical shape, the face, pond of described culturing pool has a set of rainproof cladding system being mainly made up of floating spool, motor, track, pulley, coverlay.
Culturing pool the most according to claim 5, it is characterised in that it can be applicable to the cultivation of other algae such as: the sea-farming of spirulina, spirulina, Xi Zao, the freshwater aquiculture of chlorella.
CN201510321574.3A 2015-06-12 2015-06-12 A kind of algae outdoor propagation method Pending CN106258920A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107027618A (en) * 2017-06-04 2017-08-11 刘兰喜 One bulb algae cultivating facility and its cultivation technique
CN107750836A (en) * 2017-09-19 2018-03-06 严爱平 A kind of method for the polyethylene mesh sheet shop fixtures plantation eel grass for preventing river crab destruction

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1044200A (en) * 1990-01-25 1990-08-01 轻工业部制盐工业科学研究所 Salt algae outdoor breeding method and breed pond
CN1059631A (en) * 1991-07-29 1992-03-25 天津市房地产开发经营集团塘沽总公司 Salt algae cultural method and breed pond
CN1831105A (en) * 2006-03-21 2006-09-13 中盐制盐工程技术研究院 Method for culturing 'Yuanshizao' alga in large scale outdoor, and its processing method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1044200A (en) * 1990-01-25 1990-08-01 轻工业部制盐工业科学研究所 Salt algae outdoor breeding method and breed pond
CN1059631A (en) * 1991-07-29 1992-03-25 天津市房地产开发经营集团塘沽总公司 Salt algae cultural method and breed pond
CN1831105A (en) * 2006-03-21 2006-09-13 中盐制盐工程技术研究院 Method for culturing 'Yuanshizao' alga in large scale outdoor, and its processing method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107027618A (en) * 2017-06-04 2017-08-11 刘兰喜 One bulb algae cultivating facility and its cultivation technique
CN107750836A (en) * 2017-09-19 2018-03-06 严爱平 A kind of method for the polyethylene mesh sheet shop fixtures plantation eel grass for preventing river crab destruction

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Application publication date: 20170104