CN106906143A - A kind of method for controlling carnallite algae in salt algae cultivating large area pond - Google Patents

A kind of method for controlling carnallite algae in salt algae cultivating large area pond Download PDF

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CN106906143A
CN106906143A CN201710191527.0A CN201710191527A CN106906143A CN 106906143 A CN106906143 A CN 106906143A CN 201710191527 A CN201710191527 A CN 201710191527A CN 106906143 A CN106906143 A CN 106906143A
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algae
salt
bittern
salt algae
grams
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辛乃宏
杨俊涛
李炳乾
王新磊
赵喜荣
刘颖芬
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CNSIC ENGINEERING TECHNOLOGY RESEARCH INSTITUTE Co Ltd
Jinghe County Jinghe Salinization Co Ltd
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CNSIC ENGINEERING TECHNOLOGY RESEARCH INSTITUTE Co Ltd
Jinghe County Jinghe Salinization Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

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Abstract

The invention discloses a kind of method for controlling carnallite algae in salt algae cultivating large area pond, comprise the following steps：1) to quick lime of being splashed in culturing pool, 20 24 ° of bittern of B é are introduced into culturing pool to the 20 30cm depth of waters, bittern is concentrated into 26 28 ° of B é by evaporation, and bottom of pond has salt crystal to separate out；2) pour into water and adjust bittern salinity to 20 24 ° of B é；3) by being inoculated with the salt algae Dunaliella salina of certain density, form it into population advantage, the growth of the addition control carnallite algae Dunaliella viridis of control salinity and nutritive salt, salt algae product of the production with high carotene content, cultivates last carotene carotene content and reaches 8 12 mg/litres.

Description

A kind of method for controlling carnallite algae in salt algae cultivating large area pond
Technical field
The invention belongs to salt algae Dunaliella in one kind control salt algae Dunaliella salina cultivating large areas pond The method of viridis (the salt algae is referred to as carnallite algae by us).
Background technology
Salt algae Dunaliella salina, are the unicellular microalgae of double flagellums.Its conversion of solar energy is high, and growth and breeding is fast, its The suitable salinity scope of growth is 6% to saturated bittern, and the Rapid Accumulation carrotene under salinity high is Hu in nature biotechnology Radish cellulose content highest biological variety.In addition to natural Beta-carotene, also rich in multiple biological activities such as polysaccharide, diterpene in salt algae The mineral matter of material and needed by human body, these materials have unique chemical constitution, and its efficacy effect is that terrestrial life is difficult to replace Generation, it is considered as economic algae.
When salt algae cultivating large area is carried out, there are two kinds of salt algaes in usual water body：Salt algae Dunaliella salina, one Kind cell individual is larger, and carotene carotene content accumulates salt algae high；There is a kind of salt algae Dunaliella viridis (we in addition The salt algae is referred to as carnallite algae), its cell individual is relatively small, and breeding is fast, the relatively low salt algae of carotene accumulation.In salt algae In Dunaliella salina cultivation, easily polluted by salt algae Dunaliella viridis, salt algae Dunaliella Viridis and salt algae Dunaliella salina compete nutrition and growing space, influence salt algae Dunaliella salina's Normal growth, reduces the carotene carotene content and market value of salt algae Dunaliella salina products.How salt algae is controlled Salt algae Dunaliella viridis in Dunaliella salina cultivation are one of keys of salt algae cultivation.In small area Runway recognition pond in often kill salt algae Dunaliella viridis by disinfecting, but cultivating large area Pond is difficult to use this mode.
The content of the invention
The purpose of the present invention is to overcome the deficiencies in the prior art, there is provided can be formed salt algae Dunaliella salina and plant Group's advantage, in a kind of control salt algae cultivating large area pond of control carnallite algae Dunaliella viridis growths the side of carnallite algae Method.
Technical scheme is summarized as follows：
A kind of method for controlling carnallite algae in salt algae cultivating large area pond, comprises the following steps：
1) the 20-24 ° of bittern of B é is introduced into culturing pool to the quick lime of the 15-25kg that splashed in every mu of culturing pool in proportion To the 20-30cm depth of waters, bittern is concentrated into 26-28 ° of B é using natural evaporation, and bottom of pond has salt crystal to separate out；
2) pour into water and adjust bittern salinity to 20-24 ° of B é；
3) 1/3 part of nutritive salt total amount of adding is added, salt algae Dunaliella salina to algae density 2-4 ten thousand is accessed thin Born of the same parents/milliliter, detection algae density, treats that salt algae Dunaliella salina grow into ten thousand cells/mls of 5-8, and carnallite algae daily When Dunaliella viridis numbers ratio is less than salt algae sum 10%, second 1/3 part of addition nutritive salt total amount of adding；Treat Salt algae Dunaliella salina grow into ten thousand cells/mls of 9-16, and salt algae Dunaliella viridis number ratios Less than salt algae sum 20% when, third time adds 1/3 part of nutritive salt total amount of adding, salt algae Dunaliella salina density Reach more than ten thousand cells/mls of 20-30, carry out salt algae harvesting, whole breeding process is 20- by add water to maintain salinity 24°Bé。
Preferably, nutritive salt total amount of adding is every cubic metre of water body containing 80-120 grams of nitrogen, 4.5-7 grams of phosphorus, 25-38 grams NaHCO3, 114-171 grams of CaCL2.2H2O, 1-1.7 gram of Na2EDTA and 1-2.1 grams of FeCL3.6H2O。
The method of the present invention substantially reduces carnallite algae Dunaliella in salt algae Dunaliella salina cultivation The growth of viridis, realizes that the salt algae Dunaliella salina with higher proportion are cultivated, and improves salt algae Dunaliella The cultivation efficiency and the quality of product of salina, cultivate last carotene carotene content and reach 8-12 mg/litres.
Specific embodiment
Below by specific embodiment, the present invention is further illustrated.
Embodiment 1
A kind of method for controlling carnallite algae in salt algae cultivating large area pond, comprises the following steps：
1) 22 ° of bittern of B é are introduced into culturing pool to 25cm to the quick lime of the 20kg that splashed in every mu of culturing pool in proportion The depth of water, 27 ° of B é are concentrated into using natural evaporation by bittern, and bottom of pond has salt crystal to separate out；
2) pour into water and adjust bittern salinity to 22 ° of B é；
3) 1/3 part of addition nutritive salt total amount of adding, access salt algae Dunaliella salina to 30,000 cells of algae density/ Milliliter, detection algae density, treats that salt algae Dunaliella salina grow into 70,000 cells/mls, and carnallite algae daily When Dunaliella viridis numbers ratio is less than salt algae sum 10%, second 1/3 part of addition nutritive salt total amount of adding；Treat Salt algae Dunaliella salina grow into 130,000 cells/mls, and salt algae Dunaliella viridis number ratios are small When the 20% of salt algae sum, third time adds 1/3 part of nutritive salt total amount of adding, and salt algae Dunaliella salina density reaches To 270,000 cells/mls, carotene carotene content reaches 10 mg/litres, carries out salt algae harvesting, and whole breeding process is by addition It is 20-24 ° of B é, 30 days whole culture-cycles, 22-30 DEG C of bittern per day water temperature during cultivation that water maintains salinity.
Nutritive salt total amount of adding is every cubic metre of water body containing 100 grams of nitrogen (addition is to convert into 100 grams of sodium nitrate of nitrogen), 6 Gram phosphorus (is added and converts into 6 grams of potassium dihydrogen phosphates of phosphorus), 30 grams of NaHCO3, 150 grams of CaCL2.2H2O, 1.3 grams of Na2EDTA and 1.5 gram FeCL3.6H2O。
Embodiment 2
A kind of method for controlling carnallite algae in salt algae cultivating large area pond, comprises the following steps：
1) 20 ° of bittern of B é are introduced into culturing pool to 20cm to the quick lime of the 25kg that splashed in every mu of culturing pool in proportion The depth of water, 26 ° of B é are concentrated into using natural evaporation by bittern, and bottom of pond has salt crystal to separate out；
2) pour into water and adjust bittern salinity to 24 ° of B é；
3) 1/3 part of addition nutritive salt total amount of adding, access salt algae Dunaliella salina to 20,000 cells of algae density/ Milliliter, detection algae density, treats that salt algae Dunaliella salina grow into 50,000 cells/mls, and carnallite algae daily When Dunaliella viridis numbers ratio is less than salt algae sum 10%, second 1/3 part of addition nutritive salt total amount of adding；Treat Salt algae Dunaliella salina grow into 90,000 cells/mls, and salt algae Dunaliella viridis number ratios are small When the 20% of salt algae sum, third time adds 1/3 part of nutritive salt total amount of adding, and salt algae Dunaliella salina density reaches To 200,000 cells/mls, carotene carotene content reaches 12 mg/litres, carries out salt algae harvesting, and whole breeding process is by addition It is 20-24 ° of B é, 35 days whole culture-cycles, 22-28 DEG C of bittern per day water temperature during cultivation that water maintains salinity.
Nutritive salt total amount of adding is every cubic metre of water body containing 80 grams of nitrogen (addition is to convert into 80 grams of potassium nitrate of nitrogen), 7 grams Phosphorus (addition is to convert into 7 grams of sodium dihydrogen phosphates of phosphorus), 25 grams of NaHCO3, 171 grams of CaCL2.2H2O, 1 gram of Na2EDTA and 2.1 gram FeCL3.6H2O。
Embodiment 3
A kind of method for controlling carnallite algae in salt algae cultivating large area pond, comprises the following steps：
1) 20 ° of bittern of B é are introduced into culturing pool to 30cm to the quick lime of the 15kg that splashed in every mu of culturing pool in proportion The depth of water, 28 ° of B é are concentrated into using natural evaporation by bittern, and bottom of pond has salt crystal to separate out；
2) pour into water and adjust bittern salinity to 20 ° of B é；
3) 1/3 part of addition nutritive salt total amount of adding, access salt algae Dunaliella salina to 40,000 cells of algae density/ Milliliter, detection algae density, treats that salt algae Dunaliella salina grow into 80,000 cells/mls, and carnallite algae daily When Dunaliella viridis numbers ratio is less than salt algae sum 10%, second 1/3 part of addition nutritive salt total amount of adding；Treat Salt algae Dunaliella salina grow into 160,000 cells/mls, and salt algae Dunaliella viridis number ratios are small When the 20% of salt algae sum, third time adds 1/3 part of nutritive salt total amount of adding, and salt algae Dunaliella salina density reaches To 300,000 cells/mls, carotene carotene content reaches 8 mg/litres, carries out salt algae harvesting, and whole breeding process is by adding water Maintenance salinity is 20-24 ° of B é, 20 days whole culture-cycles, 25-30 DEG C of bittern per day water temperature during cultivation.
Nutritive salt total amount of adding is every cubic metre of water body containing 120 grams of nitrogen (addition is to convert into 120 grams of urea of nitrogen), 4.5 Gram phosphorus (addition is to convert into 4.5 grams of sodium dihydrogen phosphates of phosphorus), 38 grams of NaHCO3, 114 grams of CaCL2.2H2O, 1.7 grams of Na2EDTA With 1 gram of FeCL3.6H2O。
Salt algae Dunaliella salina kinds pick up from Xinjiang Uygur Autonomous Regions's Jinghe in August, 2015 in each embodiment County Jinghe salinization Co., Ltd Ebinur Lake salt pan.
Bittern is processed from Xinjiang Uygur Autonomous Regions's Ebinur Lake lake water and by following methods in each embodiment：
1) Ebinur Lake lake water (sodium sulfate type salt lake water) is pumped into jelly nitre pond, using less than -10 DEG C of natural environment, is made There is saltcake to separate out in lake water and be deposited to jelly nitre pond bottom of pond, upper strata bittern is imported into halogen storage pool, be evaporated to 18 ° of B é.
2) when the per day water temperature of halogen storage pool reaches more than 10 DEG C, the bittern in halogen storage pool is imported into salt algae culture pond makes halogen Water depth is 30cm, using natural evaporation, bittern salinity is brought up into 25 ° of B é, and has crystallization to separate out, discharge bittern upper table towards The bittern of lower 5cm, salinity is made to being added water in remaining bittern for 22 ° of B é, obtains being suitable to salt algae Dunaliella salina cultivation Bittern.
After testing, the Cl of above-mentioned bittern-:SO4 2-Ion concentration (g/L) ratio is 4.15, Mg2+:Ca2+Ion concentration (g/L) Ratio is 8.88.

Claims (2)

1. a kind of method for controlling carnallite algae in salt algae cultivating large area pond, it is characterized in that comprising the following steps：
1) the 20-24 ° of bittern of B é is introduced into culturing pool to 20- to the quick lime of the 15-25kg that splashed in every mu of culturing pool in proportion The 30cm depth of waters, 26-28 ° of B é is concentrated into using natural evaporation by bittern, and bottom of pond has salt crystal to separate out；
2) pour into water and adjust bittern salinity to 20-24 ° of B é；
3) 1/3 part of nutritive salt total amount of adding is added, ten thousand cell/millis of salt algae Dunaliella salina to algae density 2-4 are accessed Rise, detection algae density, treats that salt algae Dunaliella salina grow into ten thousand cells/mls of 5-8, and carnallite algae daily When Dunaliella viridis numbers ratio is less than salt algae sum 10%, second 1/3 part of addition nutritive salt total amount of adding；Treat Salt algae Dunaliella salina grow into ten thousand cells/mls of 9-16, and salt algae Dunaliella viridis number ratios Less than salt algae sum 20% when, third time adds 1/3 part of nutritive salt total amount of adding, salt algae Dunaliella salina density Reach more than ten thousand cells/mls of 20-30, carry out salt algae harvesting, whole breeding process is 20- by add water to maintain salinity 24°Bé。
2. method according to claim 1, it is characterized in that the nutritive salt total amount of adding is every cubic metre of water body contains 80- 120 grams of nitrogen, 4.5-7 grams of phosphorus, 25-38 grams of NaHCO3, 114-171 grams of CaCL2.2H2O, 1-1.7 gram of Na2EDTA and 1-2.1 grams FeCL3.6H2O。
CN201710191527.0A 2017-03-28 2017-03-28 A kind of method for controlling carnallite algae in salt algae cultivating large area pond Pending CN106906143A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110964641A (en) * 2018-09-28 2020-04-07 内蒙古兰太药业有限责任公司 Dunaliella salina culture method for controlling phytoplankton
CN115039728A (en) * 2022-07-11 2022-09-13 杨九新 Method for breeding saltwater organisms in production facility for exploiting mirabilite and salt by water dissolving method

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Publication number Priority date Publication date Assignee Title
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CN105176825A (en) * 2015-10-13 2015-12-23 中盐工程技术研究院有限公司 Method for large-scale production of dunaliella salina by means of sodium sulfate salt lake and salt pan halogen pond

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KR20140015059A (en) * 2012-07-27 2014-02-06 인하대학교 산학협력단 Culture media composition of microalgae by using salt underground water or bedrock underground water including calcium ion
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110964641A (en) * 2018-09-28 2020-04-07 内蒙古兰太药业有限责任公司 Dunaliella salina culture method for controlling phytoplankton
CN110964641B (en) * 2018-09-28 2023-05-12 内蒙古兰太药业有限责任公司 Brine alga culture method for controlling phytoplankton
CN115039728A (en) * 2022-07-11 2022-09-13 杨九新 Method for breeding saltwater organisms in production facility for exploiting mirabilite and salt by water dissolving method
CN115039728B (en) * 2022-07-11 2024-04-09 杨九新 Method for cultivating salty water organisms in mirabilite salt production facility mined by water-soluble method

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Application publication date: 20170630