CN105962371A - Application of Chinese wistaria extract to preparation of health food capable of relieving physical fatigue - Google Patents
Application of Chinese wistaria extract to preparation of health food capable of relieving physical fatigue Download PDFInfo
- Publication number
- CN105962371A CN105962371A CN201610320529.0A CN201610320529A CN105962371A CN 105962371 A CN105962371 A CN 105962371A CN 201610320529 A CN201610320529 A CN 201610320529A CN 105962371 A CN105962371 A CN 105962371A
- Authority
- CN
- China
- Prior art keywords
- extract
- ethanol
- radix glycyrrhizae
- water
- reduced pressure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000284 extract Substances 0.000 title claims abstract description 70
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 235000013402 health food Nutrition 0.000 title claims abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 118
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical class CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims abstract description 98
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 68
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 47
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 28
- 238000000605 extraction Methods 0.000 claims abstract description 28
- 239000003208 petroleum Substances 0.000 claims abstract description 28
- 239000012259 ether extract Substances 0.000 claims abstract description 14
- 238000011010 flushing procedure Methods 0.000 claims abstract description 14
- 239000011347 resin Substances 0.000 claims abstract description 14
- 229920005989 resin Polymers 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 12
- 238000010828 elution Methods 0.000 claims description 26
- 238000002481 ethanol extraction Methods 0.000 claims description 26
- 230000000694 effects Effects 0.000 claims description 14
- 238000004090 dissolution Methods 0.000 claims description 13
- 238000004821 distillation Methods 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 13
- 241000202807 Glycyrrhiza Species 0.000 abstract 3
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 abstract 3
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 abstract 3
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 abstract 3
- 229940010454 licorice Drugs 0.000 abstract 3
- 238000002156 mixing Methods 0.000 abstract 2
- 239000004480 active ingredient Substances 0.000 abstract 1
- 238000007865 diluting Methods 0.000 abstract 1
- 239000003480 eluent Substances 0.000 abstract 1
- 239000002024 ethyl acetate extract Substances 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 238000010992 reflux Methods 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000009182 swimming Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000037396 body weight Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000003304 gavage Methods 0.000 description 2
- 230000033764 rhythmic process Effects 0.000 description 2
- 241001164374 Calyx Species 0.000 description 1
- 241001573881 Corolla Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 206010020112 Hirsutism Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 240000003186 Stachytarpheta cayennensis Species 0.000 description 1
- 235000009233 Stachytarpheta cayennensis Nutrition 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000014510 cooky Nutrition 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000000556 factor analysis Methods 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012109 statistical procedure Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses application of Chinese wistaria extract to preparation of health food capable of relieving physical fatigue. The Chinese wistaria extract is prepared by the following method: (a) mixing and crushing dried Chinese wistaria and licorice, performing heat reflux extraction by virtue of a 70 to 80 percent ethanol solution, mixing extracting solutions, and performing concentration until ethanol smell is removed to obtain an ethanol extracting concentrated solution; (b) diluting the ethanol extracting concentrated solution obtained in step (a) with water, and performing extraction and reduced pressure concentration sequentially with petroleum ether, ethyl acetate and water saturated n-butyl alcohol to obtain petroleum ether extract, ethyl acetate extract and n-butyl alcohol extract respectively; (c) dissolving the n-butyl alcohol extract in water, performing filtration, enriching active ingredients by virtue of AB-8 type macroporous resin, flushing a volume of 6 to 10 columns with 10 to 20 percent ethanol to remove large polar components, eluting a volume of 8 to 12 columns with 50 to 60 percent ethanol, collecting 50 to 60 percent ethanol eluent, and performing reduced pressure concentration, wherein the amount of the licorice based on the total weight of the Chinese wistaria and the licorice is 5 to 15 percent.
Description
Technical field
The present invention relates to field of food, be specifically related to the application in preparation health food for relieving physical fatigue of the Caulis wisteriae sinensis extract.
Background technology
Along with the development of society, the progress of science and technology, the quickening of rhythm of life, the requirement abundant, cultural life of material life is more and more higher, the rhythm of life of people is the most more accelerated, and tired incidence rate will necessarily surge, the most tired universal phenomenon having become adult.Fatigue is the most wide in range a kind of concept, because it is various for causing the physiology of fatigue, causes for pathological, from the point of view of the medical science of Chinese tradition and health preserving viewpoint, the deficiency of the kidney deficiency of vital energy, overwork, disharmony between QI and blood, lost in a reverie etc. be all likely to result in fatigue, and fatigue can make people the occur phenomenon such as asthenia is unable, immunity degradation, blunt, the senilism of brain response, directly and rapidly cause death unlike cancer, heart disease although tired, but, the stealthy killer as a kind of modern of harm also causes significant damage to human health.
Caulis wisteriae sinensis is the stem and leaf of leguminous plant Caulis wisteriae sinensis.Summer, autumn gather.Former plant Caulis wisteriae sinensis is fallen leaves climbing shrub.Stem is wound on his thing.Odd number winglike compound leaf, alternate；Stipule wire lanceolar, caducous；Lobule 7～11 pieces, ovum shape lanceolar or square round shape lanceolar, long 4～7 centimetres, one piece of top is bigger；Little leaf apex is tapering, the wealthy wedge shape of base portion；Close life patch fine, soft fur during children, without hair time ripe.Raceme side is raw, falls to hang down, long 15～30 centimetres；Bennet is weak, hairiness, long 1～2 centimetre；Calyx mitriform, 5 fissures, close by fine, soft fur；Corolla butterfly, bluish violet, vexil is big, outer instead, base portion has 2 appendage, ala base portion to have ear, and fossil fragments are blunt, falciform；Stamen 2 body；Style inflection, stigma top is raw.Pod length and flat, long 10～20 centimetres, close raw fine hair.Seed is oblate, 1～3.Florescence 3～April.Really phase 9～October.Cultivate in flower garden more.The ground such as distribution northeast, Shandong, Henan, Hebei, Shaanxi, Hubei, Jiangsu, Zhejiang, Sichuan, Guangdong.
Summary of the invention
It is an object of the invention to provide the application in preparation health food for relieving physical fatigue of a kind of Caulis wisteriae sinensis extract and this Caulis wisteriae sinensis extract.Caulis wisteriae sinensis extract has the effect of good alleviating physical fatigue.
Above-mentioned purpose is achieved by following technical solution:
A kind of Caulis wisteriae sinensis extract with alleviating physical fatigue effect, is prepared via a method which: dry Caulis wisteriae sinensis and Radix Glycyrrhizae are mixed by (a), pulverize, with 70 ~ 80% ethanol solution circumfluence distillation, united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 6 ~ 10 column volumes of 10 ~ 20% alcohol flushing, again with 8 ~ 12 column volumes of 50 ~ 60% ethanol elution, collect 50 ~ 60% ethanol elution, concentrating under reduced pressure；Wherein, Radix Glycyrrhizae accounts for the 5% ~ 15% of Caulis wisteriae sinensis and Radix Glycyrrhizae gross weight.
Further, the described Caulis wisteriae sinensis extract with alleviating physical fatigue effect is prepared via a method which: dry Caulis wisteriae sinensis and Radix Glycyrrhizae are mixed by (a), pulverize, with 75% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 15% alcohol flushing, then with 10 column volumes of 55% ethanol elution, collect 55% ethanol elution, concentrating under reduced pressure；Wherein, Radix Glycyrrhizae accounts for the 10% of Caulis wisteriae sinensis and Radix Glycyrrhizae gross weight.
Further, the described Caulis wisteriae sinensis extract with alleviating physical fatigue effect is prepared via a method which: dry Caulis wisteriae sinensis and Radix Glycyrrhizae are mixed by (a), pulverize, with 70% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 10 column volumes of 10% alcohol flushing, then with 12 column volumes of 50% ethanol elution, collect 50% ethanol elution, concentrating under reduced pressure；Wherein, Radix Glycyrrhizae accounts for the 5% of Caulis wisteriae sinensis and Radix Glycyrrhizae gross weight.
Further, the described Caulis wisteriae sinensis extract with alleviating physical fatigue effect is prepared via a method which: dry Caulis wisteriae sinensis and Radix Glycyrrhizae are mixed by (a), pulverize, with 80% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 6 column volumes of 20% alcohol flushing, then with 8 column volumes of 60% ethanol elution, collect 60% ethanol elution, concentrating under reduced pressure；Wherein, Radix Glycyrrhizae accounts for the 15% of Caulis wisteriae sinensis and Radix Glycyrrhizae gross weight.
The application in preparation health food for relieving physical fatigue of the above-mentioned Caulis wisteriae sinensis extract.
Advantages of the present invention:
The Caulis wisteriae sinensis extract that the present invention provides has the effect of good alleviating physical fatigue, can develop into food additive and add in food, and preparation has the health food of alleviating physical fatigue effect.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.Although the present invention being explained in detail with reference to preferred embodiment, it will be understood by those within the art that, technical scheme can be modified or equivalent, without deviating from the spirit and scope of technical solution of the present invention.
Embodiment 1: the preparation of Caulis wisteriae sinensis extract 1
A Caulis wisteriae sinensis and Radix Glycyrrhizae (accounting for gross weight 10%) that 5kg is dried by () mix, and pulverize, with 75% ethanol solution circumfluence distillation (20L × 3 time), united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 15% alcohol flushing, then with 10 column volumes of 55% ethanol elution, collect 55% ethanol elution, concentrating under reduced pressure.
Embodiment 2: the preparation of Caulis wisteriae sinensis extract 2
A Caulis wisteriae sinensis and Radix Glycyrrhizae (accounting for gross weight 5%) that 5kg is dried by () mix, and pulverize, with 70% ethanol solution circumfluence distillation (20L × 3 time), united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 10 column volumes of 10% alcohol flushing, then with 12 column volumes of 50% ethanol elution, collect 50% ethanol elution, concentrating under reduced pressure.
Embodiment 3: the preparation of Caulis wisteriae sinensis extract
A Caulis wisteriae sinensis and Radix Glycyrrhizae (accounting for gross weight 15%) that 5kg is dried by () mix, and pulverize, with 80% ethanol solution circumfluence distillation, united extraction liquid (20L × 3 time), are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butyl alcohol (3L × 3 time) extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 6 column volumes of 20% alcohol flushing, then with 8 column volumes of 60% ethanol elution, collect 60% ethanol elution, concentrating under reduced pressure.
Embodiment 4: the contrast of embodiment 1, without Radix Glycyrrhizae
A Caulis wisteriae sinensis that 5kg is dried by () is pulverized, and with 75% ethanol solution circumfluence distillation (20L × 3 time), united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 5% alcohol flushing, then with 10 column volumes of 55% ethanol elution, collect 55% ethanol elution, concentrating under reduced pressure.
Embodiment 5: the contrast of embodiment 1, Radix Glycyrrhizae accounts for the 20% of Caulis wisteriae sinensis and Radix Glycyrrhizae gross weight
A Caulis wisteriae sinensis and Radix Glycyrrhizae (accounting for gross weight 20%) that 5kg is dried by () mix, and pulverize, with 75% ethanol solution circumfluence distillation (20L × 3 time), united extraction liquid, are concentrated into and obtain ethanol extraction concentrated solution without alcohol taste；B () is by step (a) gained ethanol extraction concentrated solution dilute with water (3L), successively with petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butanol extraction (3L × 3 time), concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 25% alcohol flushing, then with 10 column volumes of 55% ethanol elution, collect 55% ethanol elution, concentrating under reduced pressure.
Embodiment 6: alleviating physical fatigue is tested
1, experimental animal and sample
60 healthy and strong male white mouses, body weight 25 ± 3g.During experiment, all animals gives cleaning grade Mus pellet and aquesterilisa freely eats.Test sample is prepared according to embodiment 1 ~ 5 method respectively, respectively obtains extract 1 ~ 5.
2, test method
2.1 dosage and packet
It is randomly divided into 6 groups, matched group and extract 1 ~ 5 group, often group 10.Extract 1 ~ 5 group respectively gavage extract 1 ~ 5, dosage be every day 200mg/kg(extract is dissolved in distilled water)；Matched group is given and isopyknic distilled water.During test, mice freely ingests and drinks water, and weighs a body weight every 2d.
2.2 swimming with a load attached to the body experiments
After being administered 30d, after last gavage 30min, put white mice to swim cylinder (100cm × 60cm × 60cm) went swimming in glass, the depth of water is not less than 30cm, water temperature 10 ± 1 DEG C, the sheet lead of rat-tail root load body weight 5%, record mice from swimming start to submerged 8s can not floatation time, the power of being recorded as exhausts the time (min).
2.3 data process
Statistical procedures result X ± S represents, uses SPSS17.0 software to carry out one factor analysis of variance process.
3, result of the test
Result of the test see table.
Upper table illustrates, the extract that the present invention provides can significantly extend the mice burden swimming time, has the effect of alleviating physical fatigue.This effect is relevant with the ratio whether adding Radix Glycyrrhizae and Radix Glycyrrhizae interpolation in raw material.
In food, add this extract, health food for relieving physical fatigue can be developed into, such as: the cookies of alleviating physical fatigue, the drinking water etc. of alleviating physical fatigue.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit protection scope of the present invention with this.It will be understood by those within the art that, technical scheme can be modified or equivalent, without deviating from essence and the protection domain of technical solution of the present invention.
Claims (5)
1. a Caulis wisteriae sinensis extract with alleviating physical fatigue effect, it is characterised in that be prepared via a method which: dry Caulis wisteriae sinensis and Radix Glycyrrhizae are mixed by (a), pulverize, with 70 ~ 80% ethanol solution circumfluence distillation, united extraction liquid, it is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 6 ~ 10 column volumes of 10 ~ 20% alcohol flushing, again with 8 ~ 12 column volumes of 50 ~ 60% ethanol elution, collect 50 ~ 60% ethanol elution, concentrating under reduced pressure；Wherein, Radix Glycyrrhizae accounts for the 5% ~ 15% of Caulis wisteriae sinensis and Radix Glycyrrhizae gross weight.
The Caulis wisteriae sinensis extract with alleviating physical fatigue effect the most according to claim 1, it is characterized in that, it is prepared via a method which: dry Caulis wisteriae sinensis and Radix Glycyrrhizae are mixed by (a), pulverize, with 75% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 8 column volumes of 15% alcohol flushing, then with 10 column volumes of 55% ethanol elution, collect 55% ethanol elution, concentrating under reduced pressure；Wherein, Radix Glycyrrhizae accounts for the 10% of Caulis wisteriae sinensis and Radix Glycyrrhizae gross weight.
The Caulis wisteriae sinensis extract with alleviating physical fatigue effect the most according to claim 1, it is characterized in that, it is prepared via a method which: dry Caulis wisteriae sinensis and Radix Glycyrrhizae are mixed by (a), pulverize, with 70% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 10 column volumes of 10% alcohol flushing, then with 12 column volumes of 50% ethanol elution, collect 50% ethanol elution, concentrating under reduced pressure；Wherein, Radix Glycyrrhizae accounts for the 5% of Caulis wisteriae sinensis and Radix Glycyrrhizae gross weight.
The Caulis wisteriae sinensis extract with alleviating physical fatigue effect the most according to claim 1, it is characterized in that, it is prepared via a method which: dry Caulis wisteriae sinensis and Radix Glycyrrhizae are mixed by (a), pulverize, with 80% ethanol solution circumfluence distillation, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；B (), by step (a) gained ethanol extraction concentrated solution dilute with water, successively with petroleum ether, ethyl acetate and water saturated n-butanol extraction, concentrating under reduced pressure, respectively obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract；C () n-butyl alcohol extract water dissolution, filters, with AB-8 type macroporous resin enrichment active component, first remove big polar component with 6 column volumes of 20% alcohol flushing, then with 8 column volumes of 60% ethanol elution, collect 60% ethanol elution, concentrating under reduced pressure；Wherein, Radix Glycyrrhizae accounts for the 15% of Caulis wisteriae sinensis and Radix Glycyrrhizae gross weight.
5. claim 1 ~ 4 arbitrary described Caulis wisteriae sinensis extract application in preparation health food for relieving physical fatigue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610320529.0A CN105962371A (en) | 2016-05-16 | 2016-05-16 | Application of Chinese wistaria extract to preparation of health food capable of relieving physical fatigue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610320529.0A CN105962371A (en) | 2016-05-16 | 2016-05-16 | Application of Chinese wistaria extract to preparation of health food capable of relieving physical fatigue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105962371A true CN105962371A (en) | 2016-09-28 |
Family
ID=56955318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610320529.0A Pending CN105962371A (en) | 2016-05-16 | 2016-05-16 | Application of Chinese wistaria extract to preparation of health food capable of relieving physical fatigue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105962371A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115634242A (en) * | 2022-10-20 | 2023-01-24 | 海南黎药生物科技有限公司 | Wisteria sinensis collateral-dredging oil for relieving swelling and pain and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101953483A (en) * | 2009-07-20 | 2011-01-26 | 胡成爱 | Fresh flower-fruit particle pumpkin juice |
| CN105030925A (en) * | 2015-09-12 | 2015-11-11 | 郭小川 | Plant extract for treating blood stasis disease and pharmaceutic preparation |
-
2016
- 2016-05-16 CN CN201610320529.0A patent/CN105962371A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101953483A (en) * | 2009-07-20 | 2011-01-26 | 胡成爱 | Fresh flower-fruit particle pumpkin juice |
| CN105030925A (en) * | 2015-09-12 | 2015-11-11 | 郭小川 | Plant extract for treating blood stasis disease and pharmaceutic preparation |
Non-Patent Citations (2)
| Title |
|---|
| 李会瑞: "酶解法提取紫藤总黄酮及自由基清除活性研究", 《河南师范大学学报》 * |
| 楚丽雅等: "甘草的抗疲劳作用研究", 《内蒙古中医药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115634242A (en) * | 2022-10-20 | 2023-01-24 | 海南黎药生物科技有限公司 | Wisteria sinensis collateral-dredging oil for relieving swelling and pain and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104304582B (en) | A kind of cyclocarya paliurus tea and preparation method thereof | |
| CN101768514B (en) | Alhagi sparsifolia extract and preparation method and application thereof | |
| CN103504299A (en) | Health food capable of relieving physical fatigue | |
| CN107823021A (en) | A kind of alligator oil moisturizing repair latex and preparation method thereof | |
| CN105341527A (en) | Fish feed added with fish feed traditional Chinese medicine additive and preparation method thereof | |
| CN113854458A (en) | Mulberry leaf solid instant beverage, intermediate and preparation method thereof | |
| WO2016183784A1 (en) | Traditional chinese medicine for treating allergic rhinitis | |
| CN105962371A (en) | Application of Chinese wistaria extract to preparation of health food capable of relieving physical fatigue | |
| CN105341526A (en) | Preparation method of fish feed added with fish feed traditional Chinese medicine additive | |
| CN105815440A (en) | Common tulip flower extract-containing fruit and vegetable fresh-keeping agent and preparation method thereof | |
| CN108079219A (en) | A kind of topical composition for treating burn and scald and preparation method thereof | |
| CN107213077A (en) | A kind of preparation method of Chinese herbal medicine ferment shampoo | |
| CN105213581A (en) | A kind of Chinese medicine formula for the treatment of liver and gall syndrome of fish and preparation method thereof | |
| CN103977112B (en) | A kind of Chinese medicine treating alcoholic liver | |
| CN105341534A (en) | Feed additive for preventing white-spot disease of parrot fishes and preparation method thereof | |
| CN104435356A (en) | Chinese herbal medicine composition capable of chicken heat stress resistance | |
| CN104845831A (en) | Formula and manufacturing technology for health-preserving health-care wine | |
| CN105341525A (en) | Fish feed traditional Chinese medicine additive | |
| CN105943746A (en) | Traditional Chinese medicine Chinese crossostepium leaf extract capable of improving growing performance of weaned pigs | |
| CN108064978A (en) | One kind wets one's whistle oil | |
| CN106581614A (en) | Ginger tea and preparation method thereof | |
| CN105902865A (en) | Traditional Chinese medicine preparation for treating tumors and preparation method thereof | |
| CN105326986A (en) | Traditional Chinese medicine for treating deafness | |
| CN105687830A (en) | Medicine for preventing and treating qi and blood deficient type piroplasmosis of cattle | |
| CN105707489A (en) | Traditional Chinese medicine scrophularia ningpoensis extract for improving productivity and muscle quality of pork pigs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160928 |