CN105961598A - Preparation method of radix puerariae yoghourt for reducing three highs and resisting tumors - Google Patents
Preparation method of radix puerariae yoghourt for reducing three highs and resisting tumors Download PDFInfo
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- CN105961598A CN105961598A CN201610313270.7A CN201610313270A CN105961598A CN 105961598 A CN105961598 A CN 105961598A CN 201610313270 A CN201610313270 A CN 201610313270A CN 105961598 A CN105961598 A CN 105961598A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/133—Fruit or vegetables
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
- A23C9/1238—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt using specific L. bulgaricus or S. thermophilus microorganisms; using entrapped or encapsulated yoghurt bacteria; Physical or chemical treatment of L. bulgaricus or S. thermophilus cultures; Fermentation only with L. bulgaricus or only with S. thermophilus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/127—Fermented milk preparations; Treatment using microorganisms or enzymes using microorganisms of the genus lactobacteriaceae and other microorganisms or enzymes, e.g. kefir, koumiss
- A23C9/1275—Fermented milk preparations; Treatment using microorganisms or enzymes using microorganisms of the genus lactobacteriaceae and other microorganisms or enzymes, e.g. kefir, koumiss using only lactobacteriaceae for fermentation in combination with enzyme treatment of the milk product; using enzyme treated milk products for fermentation with lactobacteriaceae
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C2240/00—Use or particular additives or ingredients
- A23C2240/15—Use of plant extracts, including purified and isolated derivatives thereof, as ingredient in dairy products
Abstract
The invention relates to a preparation method of radix puerariae yoghourt for reducing three highs and resisting tumors. The preparation method comprises the following steps of adding a puerarin extracting solution into yoghourt, wherein a preparation method of the puerarin extracting solution comprises the following specific steps: firstly, crushing radix puerariae to obtain radix puerariae powder with 16 meshes; secondly, uniformly mixing the dried radix puerariae powder with diatomite according to weight ratio of 1 to 1, filling an extracting pool with a mixture, and then adding 70 percent ethanol as an extracting agent, wherein the material-liquid ratio of the radix puerariae to the ethanol is 1:25; thirdly, using an automatic extracting apparatus to extract at the extraction temperature of 60DEG C for 20 minutes, and repeatedly extracting for two times; fourthly, concentrating the extracting solution by using a rotary evaporator, filtering the extracting solution and then adding the filtered solution into a sterile aqueous solution to obtain the puerarin extracting solution. According to the preparation method disclosed by the invention, the extraction rate of puerariae in the radix puerariae can be improved; in addition, the content of the puerariae is high, so that the yoghourt has health-care functions of improving cardio-cerebral blood circulation, expanding coronary artery, decreasing blood pressure, decreasing blood sugar, dispelling the effects of alcohol and protecting liver, resisting liver fibrosis and the like.
Description
Technical field
The present invention relates to the preparation method of a kind of Yoghourt, a kind of preparation method dropping three-hypers antitumor Radix Puerariae Yoghourt.
Background technology
Radix Puerariae is legume pueraria lobata or the dry root of Radix Puerariae rattan, and main component has the flavone such as general, the general unit of Semen sojae atricolor of puerarin, Semen sojae atricolor
Class material.Research shows, the osajin composition with puerarin as representative have improve heart and brain blood circulation, coronary artery dilator,
Blood pressure lowering, blood sugar lowering, relieving alcoholism and protecting the liver, anti-hepatic fibrosis etc. act on.Along with China's aged tendency of population is continuously increased, " three high " is
Through becoming the common problem that our people is healthy, owing to deterioration and the food-safety problem of environment are serious, make tumor and cancer patient anxious
Sharp increase adds, and anti-three-hypers and anti-cancer have become mainstream of society problem.Through investigation, although there being a variety of Yoghourt products in the market,
But the yogurt type products being provided simultaneously with dropping " three high " defying age does not also have.
Retrieving according to applicant, there is the technology in various food that joined by Radix Puerariae in prior art, but straight after mostly being pulverized by Radix Puerariae
Connect in addition Yoghourt, or Radix Puerariae is processed into Kudzu vane root liquid adds in Yoghourt, or add in Yoghourt after Radix Puerariae is extracted puerarin；
But these Radix Puerariae Yoghourts existing are less desirable for the curative effect of fall three-hypers, and in existing puerarin extracting method, water extraction,
Ultrasonic extraction and fermented extracted are respectively present the problems such as the highest, big to harm, the consuming time length of extraction efficiency.
Summary of the invention
The technical problem to be solved is:
The most how to improve the extraction ratio of puerarin in Radix Puerariae, and puerarin content is high, and the extracting solution containing puerarin is used for Pueraria lobota
In the preparation method of root Yoghourt, prepare fall three-hypers antitumor Radix Puerariae Yoghourt；
The fall three-hypers curative effect the most how making Radix Puerariae Yoghourt is the most prominent.
Applicant is found by research, in Radix Puerariae puerarin extraction ratio and in puerarin extracting solution, the mass concentration of puerarin is also
Be more than the most relevant to single factors, but with the solid-liquid ratio of the volume fraction of ethanol solution, Radix Puerariae and ethanol and extraction time
There are common association, puerarin extraction ratio to be improved with Extracting temperature, need volumes of aqueous ethanol mark, Radix Puerariae and ethanol
Solid-liquid ratio and extraction time and Extracting temperature carry out the setting of science；How volume fraction, Radix Puerariae and the second to ethanol solution
The solid-liquid ratio of alcohol and extraction time and Extracting temperature carry out Scientific Establishment, and the extraction ratio and the puerarin that improve puerarin carry
Take the mass concentration of puerarin in liquid.
In order to solve above technical problem, applicant proposes a kind of preparation method dropping three-hypers antitumor Radix Puerariae Yoghourt, wherein crucial
Technology is the preparation of puerarin extracting solution；Concrete technical scheme is:
A kind of preparation method dropping three-hypers antitumor Radix Puerariae Yoghourt, comprises the following steps:
(i) skimmed milk and water are mixed with the volume ratio ratio as 1:7 with weight, and add skimmed milk and water gross weight 5%-6%
After sucrose is sufficiently mixed, in 80-85 DEG C of sterilizing 10-15min, it is subsequently cooled to 35-40 DEG C, as culture matrix；
(2) using the mixed in equal amounts bacterium of purebred Streptomyces thermophilus or Bulgarian Lactobacillus or the two bacterium as leaven,
Access in described culture matrix with the inoculum concentration of 2%-5%；
Further comprising the steps of:
(iii) the preparation of puerarin extracting solution: (1) powder of Radix Puerariae is broken into the Radix Puerariae powder of 15-20 mesh；By dry Radix Puerariae powder with
Kieselguhr extracts pond by weight loading after mixing for 1:1, is subsequently adding ethanol that volume fraction is 68-72% as extraction
Take the solid-liquid ratio 1:(23-27 of agent, described Radix Puerariae and ethanol)；(3) extracting with automatic extraction instrument, extraction time is 18-22min,
Extracting temperature 58-62 DEG C, and repeat to extract 2 times；(4) utilize Rotary Evaporators concentrated extracting solution, and add after extracting solution filtration
In the aseptic aqueous solution entered, described aseptic aqueous solution is 3:1 with the volume ratio of extracting solution, obtains puerarin extracting solution；
(iv) puerarin extracting solution is joined in step (two) culture matrix and shake up, and be dispensed in sterilized yogurt bottle, by bottle cap
Tightening sealing, the addition of described puerarin extracting solution is the 0.5-1.5% of described culture matrix weight, and described yogurt bottle is put into
The calorstat of 40-42 DEG C is cultivated 3-4h, notes during cultivation observing, stop cultivating after curdled milk occurs, then proceed to 4-5 DEG C low
Temperature cold preservation 24-30 hour, reaching yogurt acidity is pH4-4.5, grumeleuse even compact, and no whey separates out, and bubble-free to obtain final product
To fall three-hypers antitumor Radix Puerariae Yoghourt.
Difference with the prior art of the present invention is: puerarin extracting solution is added containing skimmed milk and the culture matrix of bacterium by the present invention
In together participate in fermentation, the Radix Puerariae Yoghourt of gained for drop three-hypers better efficacy, it is thus achieved that beyond thought technique effect；Separately
By the puerarin extraction process of the present invention, make the extraction ratio of puerarin be improved, make the effect of above puerarin become apparent from；
As can be seen here, being added in culture matrix by puerarin extracting solution of the invention is fermented together with skimmed milk and bacterium, gained
Radix Puerariae Yoghourt is for dropping the better efficacy of three-hypers；Science of the present invention by volumes of aqueous ethanol mark, the material of Radix Puerariae and ethanol
Liquor ratio and extraction time and Extracting temperature carry out the setting of science, the extraction ratio of puerarin can be made to be greatly improved, Radix Puerariae in Radix Puerariae
Element extraction ratio be expection measured value 96.79% (expection measured value relative with actual value, such as in Radix Puerariae contain puerarin 96.74%,
It is 96.79% that expection records it containing puerarin, and that 96.79% expects measured value exactly), the puerarin extracting solution mass concentration of gained
Up to 150ug/ml.
The technical scheme that the present invention limits further is:
Further, the preparation method of aforesaid fall three-hypers antitumor Radix Puerariae Yoghourt, the preparation method of puerarin extracting solution is: (1)
After being pulverized by Radix Puerariae, obtain 16 mesh Radix Puerariae powder；(2) dry Radix Puerariae powder is mixed for 1:1 by weight with kieselguhr
Rear loading extracts pond, is subsequently adding ethanol that volume fraction is 70% as extractant, the solid-liquid ratio 1:25 of described Radix Puerariae and ethanol；
(3) extracting with automatic extraction instrument, extraction time is 20min, and Extracting temperature 60 DEG C repeats to extract 2 times；(4) rotation is utilized
Evaporimeter concentrated extracting solution, and will extracting solution filter after in the aseptic aqueous solution that joins, the volume of aseptic aqueous solution and extracting solution
Ratio is that 3:1 obtains puerarin extracting solution.
So, the present invention by volumes of aqueous ethanol mark being set as 70%, extraction time be set as 20min, and science
The solid-liquid ratio setting Radix Puerariae and ethanol is set as 1:25, and Extracting temperature is 60 DEG C, the extraction ratio of puerarin can be made to be greatly improved, Pueraria lobota
In root, puerarin extraction ratio is the 96.79% of expection measured value, the puerarin extracting solution mass concentration of gained up to 150ug/ml, as
Shown in Fig. 1, puerarin content is high, and gained puerarin extracting solution is carried out high performance liquid chromatography qualitative detection, as in figure 2 it is shown,
Through being puerarin with mark product contrast, consistent with puerarin standard substance appearance time, it was demonstrated that the principle active component of extracting solution is Radix Puerariae
Element.
The preparation method of aforesaid fall three-hypers antitumor Radix Puerariae Yoghourt, wherein automatic extraction instrument is Thermo ASE 350 auto extractive
Instrument.
The preparation method of aforesaid fall three-hypers antitumor Radix Puerariae Yoghourt, is wherein additionally added Rhizoma Steudnerae Henryanae in Yoghourt.
Advantages of the present invention: present component is simple, by the puerarin extracting solution addition culture matrix containing skimmed milk and bacterium one
Rising and participate in fermentation, the Radix Puerariae Yoghourt of gained is for dropping the better efficacy of three-hypers, and puerarin content is high, with puerarin as representative
Osajin composition has that to improve heart and brain blood circulation, coronary artery dilator, blood pressure lowering, blood sugar lowering, relieving alcoholism and protecting the liver, anti-liver fine
Dimensionizations etc. act on, so that Yoghourt has above health value；And by the puerarin extraction process of the present invention, make puerarin
Extraction ratio is improved, and makes the effect of above puerarin become apparent from.
Accompanying drawing explanation
Fig. 1 be solid-liquid ratio of the present invention and Extracting temperature in Radix Puerariae puerarin extract concentration affect schematic diagram.
Fig. 2 is puerarin canonical plotting of the present invention.
Detailed description of the invention
Embodiment 1
The present embodiment is a kind of preparation method dropping three-hypers antitumor Radix Puerariae Yoghourt, comprises the following steps:
(i) skimmed milk and water are mixed with the volume ratio ratio as 1:7 with weight, and add skimmed milk and the sugarcane of water gross weight 6%
After sugar is sufficiently mixed, in 85 DEG C of sterilizing 15min, it is subsequently cooled to 40 DEG C, as culture matrix；
(2) using the mixed in equal amounts bacterium of purebred Streptomyces thermophilus or Bulgarian Lactobacillus or the two bacterium as leaven,
Access in described culture matrix with the inoculum concentration (calculating with the weight of culture matrix) of 2%-5%；
(iii) add puerarin extracting solution after inoculation to shake up, and be dispensed in sterilized yogurt bottle and bottle cap is tightened sealing, Radix Puerariae
Addition is described culture matrix weight the 1.5% of element extracting solution；
(iv) postvaccinal yogurt bottle is put into cultivation 4h in the calorstat of 42 DEG C, note during cultivation observing, stop after curdled milk occurs
Cultivating, then proceed to 5 DEG C of deepfreezes 30 hours, reaching yogurt acidity is pH4.5, grumeleuse even compact, and no whey separates out,
Bubble-free, i.e. obtains dropping three-hypers antitumor Radix Puerariae Yoghourt；
The preparation method of puerarin extracting solution is: (1) powder of Radix Puerariae is broken into the Radix Puerariae powder of 15 mesh；By dry Radix Puerariae powder with
Kieselguhr extracts pond by weight loading after mixing for 1:1, is subsequently adding ethanol that volume fraction is 68% as extractant,
Described Radix Puerariae and the solid-liquid ratio 1:23 of ethanol；(3) extracting with automatic extraction instrument, extraction time is 18min, Extracting temperature 58
DEG C, and repeat to extract 2 times；(4) Rotary Evaporators concentrated extracting solution is utilized, and aseptic water-soluble by join after extracting solution filtration
Obtaining puerarin extracting solution in liquid, aseptic aqueous solution is 3:1 with the volume ratio of extracting solution.Wherein automatic extraction instrument is Thermo ASE
350 automatic extraction instruments.
Embodiment 2
The present embodiment is a kind of preparation method dropping three-hypers antitumor Radix Puerariae Yoghourt, comprises the following steps:
(i) skimmed milk and water are mixed with the volume ratio ratio as 1:7 with weight, and add skimmed milk and the sugarcane of water gross weight 5%
After sugar is sufficiently mixed, in 80 DEG C of sterilizing 10min, it is subsequently cooled to 35 DEG C, as culture matrix；
(2) using the mixed in equal amounts bacterium of purebred Streptomyces thermophilus or Bulgarian Lactobacillus or the two bacterium as leaven,
Inoculum concentration (calculating with the weight of culture matrix) with 2% accesses in described culture matrix；
(iii) add puerarin extracting solution after inoculation to shake up, and be dispensed in sterilized yogurt bottle and bottle cap is tightened sealing, Radix Puerariae
Addition is described culture matrix weight the 0.5% of element extracting solution；
(iv) postvaccinal yogurt bottle is put into cultivation 3h in the calorstat of 40 DEG C, note during cultivation observing, stop after curdled milk occurs
Cultivating, then proceed to 4 DEG C of deepfreezes 24 hours, reaching yogurt acidity is pH4, grumeleuse even compact, and no whey separates out,
Bubble-free, i.e. obtains dropping three-hypers antitumor Radix Puerariae Yoghourt；
The preparation method of puerarin extracting solution is: after (1) being pulverized by Radix Puerariae, obtain 16 mesh Radix Puerariae powder；(2) by dry Radix Puerariae powder
Extract pond with kieselguhr by weight loading after mixing for 1:1, be subsequently adding ethanol that volume fraction is 70% as extraction
The solid-liquid ratio 1:25 of agent, described Radix Puerariae and ethanol；(3) extracting with automatic extraction instrument, extraction time is 20min, extracts temperature
Spend 60 DEG C, repeat to extract 2 times；(4) Rotary Evaporators concentrated extracting solution, and the sterilized water that will join after extracting solution filtration are utilized
Obtaining puerarin extracting solution in solution, aseptic aqueous solution is 3:1 with the volume ratio of extracting solution.Wherein automatic extraction instrument is Thermo ASE
350 automatic extraction instruments.
The present embodiment by volumes of aqueous ethanol mark being set as 70%, Radix Puerariae be set as 1:25 with the solid-liquid ratio of ethanol, and
Extraction time be set as 20min and, what Extracting temperature carried out science is set as 60 DEG C, and the extraction ratio of puerarin can be made to be greatly improved,
In Radix Puerariae puerarin extraction ratio be expection measured value 96.79%, the puerarin extracting solution mass concentration of gained up to 150ug/ml,
As it is shown in figure 1, puerarin content is high, gained puerarin extracting solution is carried out high performance liquid chromatography qualitative detection, such as Fig. 2 institute
Show, through being puerarin with mark product contrast, consistent with puerarin standard substance appearance time, it was demonstrated that the principle active component of extracting solution is
Puerarin.
Embodiment 3
The present embodiment is a kind of preparation method dropping three-hypers antitumor Radix Puerariae Yoghourt, comprises the following steps:
(i) skimmed milk and water are mixed with the volume ratio ratio as 1:7 with weight, and add skimmed milk and the sugarcane of water gross weight 6%
After sugar is sufficiently mixed, in 83 DEG C of sterilizing 13min, it is subsequently cooled to 38 DEG C, as culture matrix；
(2) using the mixed in equal amounts bacterium of purebred Streptomyces thermophilus or Bulgarian Lactobacillus or the two bacterium as leaven,
Inoculum concentration (calculating with the weight of culture matrix) with 3% accesses in described culture matrix；
(iii) add puerarin extracting solution after inoculation to shake up, and be dispensed in sterilized yogurt bottle and bottle cap is tightened sealing, Radix Puerariae
Addition is described culture matrix weight the 1% of element extracting solution；
(iv) postvaccinal yogurt bottle is put into cultivation 3h in the calorstat of 41 DEG C, note during cultivation observing, stop after curdled milk occurs
Cultivating, then proceed to 4 DEG C of deepfreezes 27 hours, reaching yogurt acidity is pH4, grumeleuse even compact, and no whey separates out,
Bubble-free, i.e. obtains dropping three-hypers antitumor Radix Puerariae Yoghourt；
The preparation method of puerarin extracting solution is: (1) powder of Radix Puerariae is broken into the Radix Puerariae powder of 20 mesh；By dry Radix Puerariae powder with
Kieselguhr extracts pond by weight loading after mixing for 1:1, is subsequently adding ethanol that volume fraction is 72% as extractant,
Described Radix Puerariae and the solid-liquid ratio 1:27 of ethanol；(3) extracting with automatic extraction instrument, extraction time is 22min, Extracting temperature 62
DEG C, and repeat to extract 2 times；(4) Rotary Evaporators concentrated extracting solution is utilized, and aseptic water-soluble by join after extracting solution filtration
Obtaining puerarin extracting solution in liquid, aseptic aqueous solution is 3:1 with the volume ratio of extracting solution.Wherein automatic extraction instrument is Thermo ASE
350 automatic extraction instruments.
In order to preferably verify the curative effect of the present invention, below by way of curative effect group and the contrast experiment of matched group, further illustrate this
The invention fall three-hypers antitumor Radix Puerariae Yoghourt beneficial effect to three-hypers patient.
In chronological sequence three-hypers patient is randomly divided into curative effect group and matched group, curative effect group 54 example, matched group 54 example, course of disease 1-10
Year；In curative effect group 54 example, male 43 examples, female 11 example, less than 40 years old age, 12 examples, 41-60 age, 22 examples year, 60
More than year 20 examples；In matched group 54 example, male 42 examples, female 12 example, less than 40 years old age, 12 examples, age 41-60 year 22
Example, more than 60 years old 20 examples.
Diagnostic criteria: curative effect group 54 example and matched group 54 example clinical symptoms be dizziness fat, dizzy, nose heave, weak, breathe hard,
The three-hypers symptoms such as uncomfortable in chest, numb limbs and tense tendons, palpitation and insomnia, susceptible to lose temper due to restlessness.
Efficacy assessment standard: curing, transference cure, how subnormal clinical examination is；Taking a turn for the better, cardinal symptom has improvement；Do not heal,
Symptom is unchanged.
The curative effect group morning, noon and afternoon respectively take the Radix Puerariae Yoghourt 200ml of the present invention, and the matched group morning, noon and afternoon respectively take existing Radix Puerariae Yoghourt 200ml
(will puerarin or Kudzu vane root liquid directly mix with Yoghourt), three months be a course for the treatment of, is used in conjunction 3 courses for the treatment of.
Experimental result (after 3 courses for the treatment of) see table:
Table 1:
| Group | Number of cases | Cure | Take a turn for the better | Do not heal | Total cure rate % |
| Curative effect group | 54 | 49 | 4 | 1 | 90.74% |
| Matched group | 54 | 29 | 20 | 5 | 53.70% |
Can be drawn by contrast, the addition of puerarin extracting solution is participated in sending out together with in the culture matrix of bacterium by the present invention containing skimmed milk
Ferment, the Radix Puerariae Yoghourt of gained has more significant curative effect, better efficacy for fall three-hypers, it is thus achieved that beyond thought technique effect.
In addition to the implementation, the present invention can also have other embodiments.The skill that all employing equivalents or equivalent transformation are formed
Art scheme, all falls within the protection domain of application claims.
Claims (4)
1. drop a preparation method for three-hypers antitumor Radix Puerariae Yoghourt, comprise the following steps:
(i) skimmed milk and water are mixed with the volume ratio ratio as 1:7 with weight, and after the sucrose adding skimmed milk and water gross weight 5%-6% is sufficiently mixed, in 80-85 DEG C of sterilizing 10-15min, be subsequently cooled to 35-40 DEG C, as culture matrix；
(ii) using the mixed in equal amounts bacterium of purebred Streptomyces thermophilus or Bulgarian Lactobacillus or the two bacterium as leaven, access in described culture matrix with the inoculum concentration of 2%-5%；
It is characterized in that: further comprising the steps of:
(iii) the preparation of puerarin extracting solution: (1) powder of Radix Puerariae is broken into the Radix Puerariae powder of 15-20 mesh；(2) dry Radix Puerariae powder and kieselguhr are extracted pond by weight loading after mixing for 1:1, be subsequently adding ethanol that volume fraction is 68-72% as extractant, the solid-liquid ratio 1:(23-27 of described Radix Puerariae and ethanol)；(3) extracting with automatic extraction instrument, extraction time is 18-22min, Extracting temperature 58-62 DEG C, and repeats to extract 2 times；(4) utilizing in Rotary Evaporators concentrated extracting solution, and the aseptic aqueous solution that will join after extracting solution filtration, described aseptic aqueous solution is 3:1 with the volume ratio of extracting solution, obtains puerarin extracting solution；
(iv) puerarin extracting solution is joined in the most described culture matrix of step and shake up, and be dispensed in sterilized yogurt bottle, bottle cap is tightened sealing, the addition of described puerarin extracting solution is the 0.5-1.5% of described culture matrix weight, described yogurt bottle is put into cultivation 3-4h in the calorstat of 40-42 DEG C, note during cultivation observing, stop cultivating after curdled milk occurs, then 4-5 DEG C of deepfreeze is proceeded to 24-30 hour, reaching yogurt acidity is pH4-4.5, grumeleuse even compact, and no whey separates out, bubble-free, i.e. obtains dropping three-hypers antitumor Radix Puerariae Yoghourt.
2. the preparation method dropping three-hypers antitumor Radix Puerariae Yoghourt as claimed in claim 1, it is characterised in that:
The preparation method of described puerarin extracting solution is: after (1) being pulverized by Radix Puerariae, obtain 16 mesh Radix Puerariae powder；(2) dry Radix Puerariae powder and kieselguhr are extracted pond by weight loading after mixing for 1:1, be subsequently adding ethanol that volume fraction is 70% as extractant, the solid-liquid ratio 1:25 of described Radix Puerariae and ethanol；(3) extracting with automatic extraction instrument, extraction time is 20min, and Extracting temperature 60 DEG C repeats to extract 2 times；(4) utilizing in Rotary Evaporators concentrated extracting solution, and the aseptic aqueous solution that will join after extracting solution filtration, described aseptic aqueous solution is 3:1 with the volume ratio of extracting solution, obtains puerarin extracting solution.
3. the preparation method dropping three-hypers antitumor Radix Puerariae Yoghourt as claimed in claim 1 or 2, it is characterised in that: described automatic extraction instrument is Thermo ASE 350 automatic extraction instrument.
4. the preparation method dropping three-hypers antitumor Radix Puerariae Yoghourt as claimed in claim 1 or 2, it is characterised in that: described Yoghourt is additionally added Rhizoma Steudnerae Henryanae.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107535599A (en) * | 2017-09-29 | 2018-01-05 | 湖南省农产品加工研究所 | A kind of compound manual Yoghourt of root of kudzu vine purple potato and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103380820A (en) * | 2013-08-05 | 2013-11-06 | 句容茅宝葛业有限公司 | Kudzuvine root seaweed yogurt and preparation method thereof |
| CN103478250A (en) * | 2013-09-29 | 2014-01-01 | 江苏食品药品职业技术学院 | Healthcare yogurt and preparation method thereof |
| CN105360300A (en) * | 2015-12-15 | 2016-03-02 | 济南舜昊生物科技有限公司 | Blood-glucose-reducing yogurt and preparation method of the blood-glucose-reducing yogurt |
-
2016
- 2016-05-12 CN CN201610313270.7A patent/CN105961598A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103380820A (en) * | 2013-08-05 | 2013-11-06 | 句容茅宝葛业有限公司 | Kudzuvine root seaweed yogurt and preparation method thereof |
| CN103478250A (en) * | 2013-09-29 | 2014-01-01 | 江苏食品药品职业技术学院 | Healthcare yogurt and preparation method thereof |
| CN105360300A (en) * | 2015-12-15 | 2016-03-02 | 济南舜昊生物科技有限公司 | Blood-glucose-reducing yogurt and preparation method of the blood-glucose-reducing yogurt |
Non-Patent Citations (4)
| Title |
|---|
| 曹雄，等: ""葛根素醇提工艺的优化"", 《食品与机械》 * |
| 李亚军等: "葛根凝固型酸奶发酵工艺研究", 《食品与生物技术学报》 * |
| 王霞等: "葛根果汁酸乳发酵饮料的研制", 《黑龙江八一农垦大学学报》 * |
| 田启建等: "《湘西主要特色药用植物栽培与利用》", 31 May 2015 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107535599A (en) * | 2017-09-29 | 2018-01-05 | 湖南省农产品加工研究所 | A kind of compound manual Yoghourt of root of kudzu vine purple potato and preparation method thereof |
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Application publication date: 20160928 |