A kind of cultural method of Auricularia polytricha (Mout) Sacc.
Technical field
The present invention relates to field of planting, particularly to the cultural method of a kind of Auricularia polytricha (Mout) Sacc..
Background technology
Auricularia polytricha (Mout) Sacc. is also known as Auricularia polytricha (Mout) Sacc., and color and luster is dark brown, and quality is soft, and delicious flavour is nutritious, can element can meat or fish, in Auricularia
The content of ferrum is the abundantest, therefore often eats Auricularia and can nourish blood and preserve youthful looks, and it is ruddy to make us skin, radiant, and it is lean to prevent and treat iron-deficient
Blood;The vitamin K that Auricularia contains, can maintain the normal level of internal thrombin, prevent hemorrhage;Colloid in Auricularia can be residual
Stay the dust in digestion, impurity absorption puts together and excretes, thus plays clearing stomach and wash the effect of intestinal, simultaneously
The endogenous foreign body such as cholelithiasis, renal calculus is also had and more significantly dissolves function;Also help digest fiber substance function,
The foreign body such as the hair being difficult to digest eaten unintentionally, rice husk, wood slag, sand, metal fillings there are dissolving and molten effect, because of
This, be the indispensable health food of mine, chemical industry and textile worker.Auricularia contains anti-tumor active substance, can enhancing body
Immunity, often eating can cancer-resisting.Auricularia polytricha (Mout) Sacc. is tender and crisp good to eat, like Umbrella Rhopilema esculenta, can cold and dressed with sauce, fry, quick-fried soup, be deeply subject to
Consumer is liked;Its wild Auricularia polytricha (Mout) Sacc. be distributed mainly on Heilungkiang, Jilin, Fujian, Taiwan, Hubei, Guangdong, Guangxi,
The ground such as Sichuan, Guizhou, Yunnan, are grown on the rotten wood of more than the 120 kind of broad leaf trees such as oak, poplar, banyan, Chinese scholartree, single raw or all living creatures.
And market is constantly increasing for the demand of Auricularia polytricha (Mout) Sacc., current artificial implantation is based on Duan Mu's with Bag Material, in the whole nation
Various places all can carry out artificial culture, and the factor affecting Auricularia polytricha (Mout) Sacc. nutrition, quality and yield is also carried out by those skilled in the art
Research, finds that different plantation formula materials and cultural method are on the impact of Auricularia polytricha (Mout) Sacc. nutrition, quality and yield the biggest.
Therefore, for those skilled in the art, need the implantation methods developing the growth of a kind of applicable Auricularia polytricha (Mout) Sacc. badly.
Summary of the invention
In view of foregoing, it is necessary to provide the cultural method of a kind of Auricularia polytricha (Mout) Sacc., the method can be prepared specially by the most compounding raw material
Cultivation matrix plant, and cultivated by more novel inoculation nested fashion, can promote that Auricularia polytricha (Mout) Sacc. increases production, and carries
The overall quality of high Auricularia polytricha (Mout) Sacc..
For reaching above-mentioned purpose, the technical solution adopted in the present invention is:
A kind of cultural method of Auricularia polytricha (Mout) Sacc., including step in detail below:
(1) preparation of culture medium: the raw material of culture medium includes Pericarpium Musae 8-15 part, one-level broken wood shavings 5-15 part, two grades of broken planes
Flower 5-10 part, Folium Crataegi 5-15 part, Spartina alterniflora's 5-15 part, Shell of Water Chestnut 5-15 part, seedpod of the lotus 3-10 part, soy sauce waste residue
3-8 part, precipitated calcium carbonate 3-8 part, Klinit dregs 1-10 part, rape flower straw 1-10 part, analysis for soybean powder 3-5 part, marchantia
1-8 part and Testa Tritici 1-5 part;
Above-mentioned raw materials is prepared from Auricularia polytricha (Mout) Sacc. culture medium through following concrete steps:
A Feedstock treating: one-level, two grades of broken wood shavings are classified and are exposed to the sun 2-3 days under the sun;By Shell of Water Chestnut and Pericarpium Musae also in too
After being exposed to the sun under sun 3-4 days, difference flour is to 1-2cm particle diameter;Spartina alterniflora, the seedpod of the lotus, Folium Crataegi and ground wire are dried respectively to hands
Hold amphidromic sense, be then respectively crushed into 1-2cm particle diameter;Stir through granular mill after rape flower straw under high temperature sterilizing-drying
After mixing, obtain the solid particle that particle diameter is 0.2-0.4cm;Soy sauce waste residue is processed to salinity less than 1% through the desalinization of soil by flooding or leaching;
By Klinit dregs through high temperature acidified, control pH=5-6;
B fermentation process: according to weight part ratio, by the broken wood shavings of one-level after step a processes, Shell of Water Chestnut, Pericarpium Musae, mutually
Hua meter Cao, the seedpod of the lotus, Folium Crataegi, rape flower straw, soy sauce waste residue, Klinit dregs and ground wire, with Testa Tritici, precipitated calcium carbonate and
Analysis for soybean powder mix and blend, adds lime cream and water, regulates pH to 5-7, controls moisture 60-70%, obtains fermentation material,
Fermentation material is built heap fermentation 18-28h, fermentation temperature 40-50 DEG C, the most available;
C feeds: two grades of broken wood shavings after processing in step a use distilled water regulation moisture to be 50-60%, are then charged into
In culture bag, adjust two grades of wood shavings formation hollow forms and be close to outside the internal layer of culture bag, after the most again step b having been fermented
Fermentation material be filled in hollow position, make fermentation material be wrapped in two grades of broken wood shavings, then sealing obtain packed compost;
D sterilizing: by packed compost steam sterilization 10-18h at 100 DEG C, i.e. prepare Auricularia polytricha (Mout) Sacc. culture medium;
(2) culture medium inoculated and cultivation management: " one " font is followed closely in the inoculation being inoculated with Auricularia polytricha (Mout) Sacc. strain and is inoculated in cultivation
On base bacterium bag, two adjacent plant nail between be spaced apart the 1/3 of culture medium bacterium bag length, then by postvaccinal culture medium bacterium bag
Up esting becomes the inoculation nail between " 1 " font, and neighbouring culture medium bacterium bag to arrange in triangle disposition, if re-using
In culture medium bacterium bag after esting is fixed on canopy body by dry bar fixed mount and under the film layer that shelters from heat or light;Finally carry out cultivation management, until
Auricularia polytricha (Mout) Sacc. sporophore fruiting is gathered.
In the present invention, further illustrate, also include the structure cultivating booth, concretely comprise the following steps: use some support besieged cities
The canopy body of long 10-15m × wide 5-6m × high 6-7m, canopy body surrounding first covers the film that shelters from heat or light and is then covered by black film, re-uses locking strip
Surrounding in canopy body middle and upper part joins end to end successively and is fixed by the black film of covering, and is provided with some load-bearing in canopy body medium position
Support, and support bracket and canopy body length of side parallel arrangement;Described support bracket aspect ratio support is high;Between support bracket and support
Forming Yang Peng and cloudy canopy, Yang Peng and cloudy canopy to arrange back-to-back, and be interconnected, the roofing of Yang Peng tilts towards arranging in the east, cloudy
The roofing of canopy tilts towards west setting, and the ridge of Yang Peng, higher than the ridge of cloudy canopy, is provided with vent passages between Yang Peng and cloudy canopy,
Vent passages is coated with black film, the roofing of Yang Peng and cloudy canopy is all covered with black film, is from top to bottom disposed with in described sun canopy
Light-transmissive film layer, foam sheet and the film layer that shelters from heat or light, and light-transmissive film layer is close to the roofing of Yang Peng, is from top to bottom disposed with in cloudy canopy
Light-transmissive film layer, foam sheet and the film layer that shelters from heat or light, and light-transmissive film layer is close to the roofing of cloudy canopy;
In the present invention, further illustrating, the ridge of described Yang Peng is on same vertical with the ridge of cloudy canopy, described sun
Difference in height between ridge and the ridge of cloudy canopy of canopy is 20-50cm.
In the present invention, further illustrate, ground level external less than canopy after also carrying out pit digging, and pit digging in described canopy body
30-40cm。
In the present invention, further illustrating, the periphery of described canopy body is additionally provided with dewatering ditch.
In the present invention, further illustrating, the concrete mode of described training orientation is: the humidity controlled in cultivation booth is
85%-95%, temperature is 15-20 DEG C, and after covering with mycelia in culture medium bacterium bag by the film every day of sheltering from heat or light of canopy body surrounding morning 10
O'clock rolling to 12 o'clock period, remaining time puts down covering, until stopping after Auricularia polytricha (Mout) Sacc. fruiting;Will after Auricularia polytricha (Mout) Sacc. fruiting 3-8 days
The black film covered on the roofing of Yang Peng is rolled;When after Auricularia polytricha (Mout) Sacc. fruiting 9-16 days by the foam sheet dismounting in sun canopy;Work as Auricularia polytricha (Mout) Sacc.
The black film that canopy body surrounding covers was rolled after 10-14 days by fruiting period in the 3 o'clock to 5 o'clock afternoon in 10 o'clock to 12 o'clock every morning and,
Remaining time puts down covering, and so circulation is until Auricularia polytricha (Mout) Sacc. sporophore fruiting is gathered.
In the present invention, further illustrate, the length of the broken wood shavings of described one-level, thickness, wide size be 25-55 × 0.5-1.2 ×
35-75mm;The length of described two grades of broken wood shavings, thickness, wide size are 3-5 × 0.1-0.13 × 0.15-0.18mm.
In the present invention, further illustrating, the number of plies of described " 1 " font esting is 6-10 layer.
The method have the advantages that
1, the method for the present invention can be by the sun-cloudiness shed of design structure uniqueness, it is possible to keep the darkness in canopy body before Auricularia polytricha (Mout) Sacc. fruiting
And temperature, be conducive to the mycelia of inoculation quickly to increase;After Auricularia polytricha (Mout) Sacc. fruiting, the black film in sun shed face can be raised so that sun
Sunlight can be injected in shed face, when the sun rises, injects from the positive shed face tilted, and is first irradiated to the middle and upper part at cloudy canopy, makes
Obtain transpiration a whole night steam of middle and upper part in canopy body to warm so that in cloudy canopy, the atmospheric density of middle and upper part diminishes, then canopy body
Interior air themperature relatively low (density is bigger) will flow middle and upper part in cloudy canopy, when the air of Yang Peng is to cloudy canopy middle and upper part flowing,
The air external from vent passages and canopy is swapped by the cold air of a part, and according to this, the air in canopy body will gradual warm up,
The air ambient in canopy body, humidity and temperature can be adjusted so that Auricularia polytricha (Mout) Sacc. grows the poorest, it is possible to grow abundant
Sporophore, it is also possible to make canopy body have ozone and enough oxygen quickly grows by Auricularia polytricha (Mout) Sacc., postpone over time,
The bottom and direct projection of injecting cloudy canopy from the middle and upper part of cloudy canopy successively are entered in sun canopy by sunlight so that sun canopy the moon canopy all obtains sunlight and irradiates,
Grow antibacterial in preventing canopy body, provide good growing environment to Auricularia polytricha (Mout) Sacc., it is ensured that the cultivating rate of sporophore;As sporophore Zhang Zhiyi
After sizing, by the cystosepiment dismounting in sun canopy so that the sunlight light transmittance in sun canopy increases, needed for meeting sporophore growth
Amount of sunlight, the Auricularia polytricha (Mout) Sacc. in the Auricularia polytricha (Mout) Sacc. planted in promoting sun canopy canopy more cloudy than plantation grows faster, and stagger Yang Peng and cloudy canopy Auricularia polytricha (Mout) Sacc.
Collecting time, it is ensured that the Auricularia polytricha (Mout) Sacc. gathered can be sold in the case of rising trend is the most delicious, prevents from disposably gathering in a large number,
Supply exceed demand, and the Auricularia polytricha (Mout) Sacc. of waste peasant household can only dry and sell voluntarily, and the time is long, and cost is high, and the problem that price is low occurs.
2, present invention employs inoculation nail " one " font inoculation, inoculation nail joint kind is simple to operation, during esting so that up and down
Inoculation between layer bacterium bag is followed closely in triangle disposition, can stagger growing sporophore growth, relatively utilize the side gathered from two ends fruiting
Formula, higher in the inoculation method yield utilizing the identical space present invention, it is not only and increases income from space utilization, also inhale from sunlight
Receiving top grade word and stagger that sporophore can be made to absorb sunlight is identical, rising trend is homogeneous, it is possible to the bacterium bag fruiting time making upper strata is fast, when adopting
After receiving the sporophore on upper strata, lower floor starts to absorb sunlight in a large number and then gathers, and staggering time is gathered, it is possible to enter for the market demand
Row is gathered, and will cater to market, it is to avoid cause the loss that marketing is failed.
3, the cultivation matrix that the present invention uses, the present invention with Testa Tritici, analysis for soybean powder, broken wood shavings, Spartina alterniflora, precipitated calcium carbonate is
Base material mainly provides carbon and nitrogen sources, wherein with addition of adventitious plant Spartina alterniflora, it is rich in a large amount of fibers, protein, trace unit
The nutrient substance such as element, organic substance, using the teaching of the invention it is possible to provide the nutrient substance required for Auricularia polytricha (Mout) Sacc. trophophase, additionally provide denizen
Excessive multiplication causes the solution new way of zonal environmental problem;Testa Tritici provides cellulose and vitamin(e) B group, rich in analysis for soybean powder
Containing substantial amounts of protein, fat and carbohydrate, culture medium also compounds rape flower straw, ground wire and Folium Crataegi, he
All containing Brassica campestris L element sterol in is the quick merisis of strain of sterols hormone energy challenge inoculation, differentiation, thus shortens fruiting
Cycle;Containing dietary fibers such as substantial amounts of pectin, oligosaccharide, cellulose, hemicellulose, lignins in Pericarpium Musae, possibly together with
The nutritional labelings such as protein, water-soluble sugar, fat, multivitamin and plurality of inorganic salt, the content of Ca, Mg, P, K is very
Abundant, carbon nitrogen source needed for edible fungi growth can not only be provided, this also provides a new processing method, warp to agricultural wastes
Ji environmental protection;In soy sauce waste material containing the tissue of microorganism and metabolite, fermentation raw material, possibly together with moisture, crude protein, thick fat
Fat, reducing sugar, salinity, calcium, phosphorus, ash grade composition, do not contain only the nutrient substance on basis, moreover it is possible to make full use of microorganism
Tissue in containing nutrient substance such as the sugar needed for edible fungi growth, alcohol, organic acid, soy sauce waste material is carried out the process of desalination,
Ensure that mixed overall raw material salt content is maintained in the salt content environment of the growth beneficially eating mycelia, thus shorten
After inoculation, mycelia covers with the cycle in culture medium;The seedpod of the lotus contains various nutrient substance, also has certain water absorption, is beneficial to improve
Mixed raw material water absorbing properties, Extending culture base has more longlasting humidity environment, promotes the growth of edible fungi;Klinit dregs
Retentiveness is good, good permeability, beneficially mycelial growth;With addition of Shell of Water Chestnut provide carbon nitrogen source, adjust overall broth carbon nitrogen source
Ratio, moreover it is possible to staphylococcus aureus and colibacillary growth and grow in suppression culture medium, it is provided that the life of good edible fungi
Long environment, reduces the disease of Auricularia polytricha (Mout) Sacc., in sum, the raw material that the present invention uses, complements each other, complement auxotrophic, moreover it is possible to pre-
Disease prevention, starts to prevent source of disease from Ji Yuan, it is ensured that Auricularia polytricha (Mout) Sacc. quality, improves output capacity.
4, the preparation method of the present invention is simple, easily operation, easy to spread;And the present invention also by raw material xylose alcohol slag through peracid treatment
After its cellulose of Klinit dregs, hemicellulose, the nutrition Partial digestion such as lignin, beneficially mycelia absorb, after mixing
The adjusted effect of pH value of whole culture medium, be more suitable for Auricularia polytricha (Mout) Sacc. fruiting;" two grades of wood shavings bags are used when pack processes
Wrap up in fermentation material " mode, first, as fermentation material effect of heat-insulation layer after inoculation Auricularia polytricha (Mout) Sacc. inoculation bacterium, second, when covering with
When mycelia starts fruiting, play certain inhibition, it is possible to increase the quality of the Auricularia polytricha (Mout) Sacc. of fruiting, because arranging when fruiting
One obstruction, will the Auricularia polytricha (Mout) Sacc. of fruiting under inertial force, burst out stronger vigor, from research and result of the test
Understand the biologos that suitably can improve Auricularia polytricha (Mout) Sacc. to the certain obstruction of fruiting increase.
Accompanying drawing explanation
Fig. 1 is the structural representation in portion in canopy body of the present invention;
Fig. 2 is the structural representation of Auricularia polytricha (Mout) Sacc. planting greenhouse of the present invention;
Fig. 3 is cultivating bacteria bag of the present invention, stacked configuration schematic diagram;
In figure, 1 vent passages, 2 cloudy canopies, 3 light-transmissive film layer, 4 supports, 5 foam sheet, 6 shelter from heat or light film layer, 7 Auricularia polytricha (Mout) Sacc.s trainings
Support base bacterium bag, 8 support brackets, 9 Yang Peng, 10 black films, 11 locking strips, 12 gutters, 13 fixed mounts, inoculate nail 14.
Following detailed description of the invention will further illustrate the present invention in conjunction with above-mentioned accompanying drawing.
Detailed description of the invention
Embodiment 1:
A kind of cultural method of Auricularia polytricha (Mout) Sacc., including step in detail below:
(1) using the canopy body of some support besieged city long 10m × wide 5m × high 6m, canopy body surrounding first covers the film that shelters from heat or light and then covers
Lid black film, re-uses the locking strip surrounding in canopy body middle and upper part and joins end to end successively and fixed by the black film of covering, and in the middle part of canopy body
Position is provided with some support brackets, and support bracket and canopy body length of side parallel arrangement;Described support bracket aspect ratio support is high;
Forming Yang Peng and cloudy canopy, Yang Peng and cloudy canopy between support bracket and support to arrange back-to-back, and be interconnected, the roofing of Yang Peng inclines
Tiltedly towards arranging in the east, the roofing inclination of cloudy canopy is arranged towards west, and the ridge of Yang Peng is in same vertical with the ridge of cloudy canopy
On face, the ridge of Yang Peng is higher than the ridge of cloudy canopy, and the difference in height between ridge and the ridge of cloudy canopy of Yang Peng is that 20cm forms ventilation
Passage, vent passages is coated with black film, the roofing of Yang Peng and cloudy canopy is all covered with black film, in described sun canopy the most successively
It is provided with light-transmissive film layer, foam sheet and the film layer that shelters from heat or light, and light-transmissive film layer is close to the roofing of Yang Peng, in cloudy canopy the most successively
It is provided with light-transmissive film layer, foam sheet and the film layer that shelters from heat or light, and light-transmissive film layer is close to the roofing of cloudy canopy, also carries out pit digging in canopy body,
And after pit digging, it being less than the ground level 30cm that canopy is external, the periphery of canopy body is additionally provided with dewatering ditch;
(2) preparation of culture medium: the raw material of culture medium includes Pericarpium Musae 8 parts, long, thick, wide size is 25 × 0.5 × 35mm
The broken wood shavings of one-level 5 parts, long, thick, wide size is the broken wood shavings of 3 × 0.1 × 0.15mm bis-grades 5 parts, and Folium Crataegi 5 parts is spent mutually
Rice grass 5 parts, Shell of Water Chestnut 5 parts, the seedpod of the lotus 3 parts, 3 parts of soy sauce waste residue, precipitated calcium carbonate 3 parts, Klinit dregs 1 part, Brassica campestris L
Chopped straw stalk 1 part, analysis for soybean powder 3 parts, marchantia 1 part and 1 part of Testa Tritici;
Raw material is prepared from through following concrete steps:
A Feedstock treating: one-level, two grades of broken wood shavings are classified and are exposed to the sun under the sun 2 days;By Shell of Water Chestnut and Pericarpium Musae also in the sun
Under be exposed to the sun 3 days after respectively flour to 1cm particle diameter;Spartina alterniflora, the seedpod of the lotus, Folium Crataegi and ground wire are dried to holding amphidromic respectively
Sense, is then respectively crushed into 1cm particle diameter;By after rape flower straw under high temperature sterilizing-drying through granular mill stir after,
It is the solid particle of 0.2cm to particle diameter;Soy sauce waste residue is processed to salinity less than 1% through the desalinization of soil by flooding or leaching;By Klinit dregs
Through high temperature acidified, control pH=5;
B fermentation process: according to weight part ratio, by the broken wood shavings of one-level after step a processes, Shell of Water Chestnut, Pericarpium Musae, mutually
Hua meter Cao, the seedpod of the lotus, Folium Crataegi, rape flower straw, soy sauce waste residue, Klinit dregs and ground wire, with Testa Tritici, precipitated calcium carbonate and
Analysis for soybean powder mix and blend, adds lime cream and water, regulates pH to 5, controls moisture 60%, obtains fermentation material, will send out
Ferment material build heap fermentation 18h, fermentation temperature 40 DEG C, the most available;
C feeds: two grades of broken wood shavings after processing in step a use distilled water regulation moisture to be 50%, are then charged into cultivating
In bag, adjust two grades of wood shavings formation hollow forms and be close to outside the internal layer of culture bag, sending out after the most again step b having been fermented
Ferment material is filled in hollow position, makes fermentation material be wrapped in two grades of broken wood shavings, and then sealing obtains packed compost;
D sterilizing: by packed compost steam sterilization 10h at 100 DEG C, i.e. prepare Auricularia polytricha (Mout) Sacc. culture medium;
(3) culture medium inoculated and cultivation management: " one " font is followed closely in the inoculation being inoculated with Auricularia polytricha (Mout) Sacc. strain and is inoculated in cultivation
On base bacterium bag, two adjacent plant nail between be spaced apart the 1/3 of culture medium bacterium bag length, then by postvaccinal culture medium bacterium bag
" 1 " font that up esting becomes the number of plies to be 6 layers, and between neighbouring culture medium bacterium bag inoculation nail in triangle disposition arrange
Cloth, re-uses in the culture medium bacterium bag after esting is fixed on canopy body by some fixed mounts and under the film layer that shelters from heat or light;Then control to plant
Humidity in training booth is 85%, and temperature is 15 DEG C, and by every for the film that shelters from heat or light of canopy body surrounding after covering with mycelia in culture medium bacterium bag
It rolls period in the 10 o'clock to 12 o'clock morning in, and remaining time puts down covering, until stopping after Auricularia polytricha (Mout) Sacc. fruiting;Go out at Auricularia polytricha (Mout) Sacc.
The black film covered on the roofing of Yang Peng was rolled after 3 days by mushroom;When after Auricularia polytricha (Mout) Sacc. fruiting 9 days by the foam sheet dismounting in sun canopy;
When black film canopy body surrounding covered after Auricularia polytricha (Mout) Sacc. fruiting 10 days is period in the 3 o'clock to 5 o'clock afternoon in 10 o'clock to 12 o'clock every morning and
Rolling, remaining time puts down covering, and so circulation is until Auricularia polytricha (Mout) Sacc. sporophore fruiting is gathered.
Embodiment 2:
A kind of cultural method of Auricularia polytricha (Mout) Sacc., including step in detail below:
(1) using the canopy body of some support besieged city long 15m × wide 6m × high 7m, canopy body surrounding first covers the film that shelters from heat or light and then covers
Lid black film, re-uses the locking strip surrounding in canopy body middle and upper part and joins end to end successively and fixed by the black film of covering, and in the middle part of canopy body
Position is provided with some support brackets, and support bracket and canopy body length of side parallel arrangement;Described support bracket aspect ratio support is high;
Forming Yang Peng and cloudy canopy, Yang Peng and cloudy canopy between support bracket and support to arrange back-to-back, and be interconnected, the roofing of Yang Peng inclines
Tiltedly towards arranging in the east, the roofing inclination of cloudy canopy is arranged towards west, and the ridge of Yang Peng is in same vertical with the ridge of cloudy canopy
On face, the ridge of Yang Peng is higher than the ridge of cloudy canopy, and the difference in height between ridge and the ridge of cloudy canopy of Yang Peng is that 50cm forms ventilation
Passage, vent passages is coated with black film, the roofing of Yang Peng and cloudy canopy is all covered with black film, in described sun canopy the most successively
It is provided with light-transmissive film layer, foam sheet and the film layer that shelters from heat or light, and light-transmissive film layer is close to the roofing of Yang Peng, in cloudy canopy the most successively
It is provided with light-transmissive film layer, foam sheet and the film layer that shelters from heat or light, and light-transmissive film layer is close to the roofing of cloudy canopy, also carries out pit digging in canopy body,
And after pit digging, it being less than the ground level 40cm that canopy is external, the periphery of canopy body is additionally provided with dewatering ditch;
(2) preparation of culture medium: the raw material of culture medium includes Pericarpium Musae 15 parts, long, thick, wide size is 55 × 1.2 × 75mm
The broken wood shavings of one-level 15 parts, long, thick, wide size is the broken wood shavings of 5 × 0.13 × 0.18mm bis-grades 10 parts, Folium Crataegi 15 parts,
Spartina alterniflora 15 parts, Shell of Water Chestnut 15 parts, the seedpod of the lotus 10 parts, 8 parts of soy sauce waste residue, precipitated calcium carbonate 8 parts, Klinit dregs 10
Part, rape flower straw 10 parts, analysis for soybean powder 5 parts, marchantia 8 parts and 5 parts of Testa Tritici;
Raw material is prepared from through following concrete steps:
A Feedstock treating: one-level, two grades of broken wood shavings are classified and are exposed to the sun under the sun 3 days;By Shell of Water Chestnut and Pericarpium Musae also in the sun
Under be exposed to the sun 4 days after respectively flour to 2cm particle diameter;Spartina alterniflora, the seedpod of the lotus, Folium Crataegi and ground wire are dried to holding amphidromic respectively
Sense, is then respectively crushed into 2cm particle diameter;By after rape flower straw under high temperature sterilizing-drying through granular mill stir after,
It is the solid particle of 0.4cm to particle diameter;Soy sauce waste residue is processed to salinity less than 1% through the desalinization of soil by flooding or leaching;By Klinit dregs warp
Cross high temperature acidified, control pH=6;
B fermentation process: according to weight part ratio, by the broken wood shavings of one-level after step a processes, Shell of Water Chestnut, Pericarpium Musae, mutually
Hua meter Cao, the seedpod of the lotus, Folium Crataegi, rape flower straw, soy sauce waste residue, Klinit dregs and ground wire, with Testa Tritici, precipitated calcium carbonate and
Analysis for soybean powder mix and blend, adds lime cream and water, regulates pH to 7, controls moisture 70%, obtains fermentation material, will send out
Ferment material build heap fermentation 28h, fermentation temperature 50 DEG C, the most available;
C feeds: two grades of broken wood shavings after processing in step a use distilled water regulation moisture to be 60%, are then charged into cultivating
In bag, adjust two grades of wood shavings formation hollow forms and be close to outside the internal layer of culture bag, sending out after the most again step b having been fermented
Ferment material is filled in hollow position, makes fermentation material be wrapped in two grades of broken wood shavings, and then sealing obtains packed compost;
D sterilizing: by packed compost steam sterilization 18h at 100 DEG C, i.e. prepare Auricularia polytricha (Mout) Sacc. culture medium;
(3) culture medium inoculated and cultivation management: " one " font is followed closely in the inoculation being inoculated with Auricularia polytricha (Mout) Sacc. strain and is inoculated in cultivation
On base bacterium bag, two adjacent plant nail between be spaced apart the 1/3 of culture medium bacterium bag length, then by postvaccinal culture medium bacterium bag
" 1 " font that up esting becomes the number of plies to be 10 layers, and the inoculation nail between neighbouring culture medium bacterium bag is in triangle disposition
Arrangement, re-uses in the culture medium bacterium bag after esting is fixed on canopy body by some fixed mounts and under the film layer that shelters from heat or light;Then control
Humidity in cultivation booth is 95%, and temperature is 20 DEG C, and by the film that shelters from heat or light of canopy body surrounding after covering with mycelia in culture medium bacterium bag
Every day is rolled period in the 10 o'clock to 12 o'clock morning in, and remaining time puts down covering, until stopping after Auricularia polytricha (Mout) Sacc. fruiting;At Auricularia polytricha (Mout) Sacc.
The black film covered on the roofing of Yang Peng was rolled after 8 days by fruiting;When after Auricularia polytricha (Mout) Sacc. fruiting 16 days by the foam sheet dismounting in sun canopy;
When black film canopy body surrounding covered after Auricularia polytricha (Mout) Sacc. fruiting 14 days is period in the 3 o'clock to 5 o'clock afternoon in 10 o'clock to 12 o'clock every morning and
Rolling, remaining time puts down covering, and so circulation is until Auricularia polytricha (Mout) Sacc. sporophore fruiting is gathered.
Embodiment 3:
A kind of cultural method of Auricularia polytricha (Mout) Sacc., including step in detail below:
(1) using the canopy body of some support besieged city long 12m × wide 5.5m × high 6.5m, it is right that canopy body surrounding first covers the film that shelters from heat or light
Rear covering black film, re-uses the locking strip surrounding in canopy body middle and upper part and joins end to end successively and fixed by the black film of covering, and in canopy body
Medium position is provided with some support brackets, and support bracket and canopy body length of side parallel arrangement;Described support bracket aspect ratio is propped up
Frame is high;Form Yang Peng and cloudy canopy, Yang Peng and cloudy canopy between support bracket and support to arrange back-to-back, and be interconnected, Yang Peng's
Roofing tilts towards arranging in the east, and the roofing inclination of cloudy canopy is arranged towards west, and the ridge of Yang Peng is in same with the ridge of cloudy canopy
On individual vertical, the ridge of Yang Peng is higher than the ridge of cloudy canopy, and the difference in height between ridge and the ridge of cloudy canopy of Yang Peng is 35cm shape
Becoming vent passages, vent passages is coated with black film, the roofing of Yang Peng and cloudy canopy is all covered with black film, by up in described sun canopy
Under be disposed with light-transmissive film layer, foam sheet and the film layer that shelters from heat or light, and light-transmissive film layer is close to the roofing of Yang Peng, by up in cloudy canopy
Under be disposed with light-transmissive film layer, foam sheet and the film layer that shelters from heat or light, and light-transmissive film layer is close to the roofing of cloudy canopy, also carries out in canopy body
Ground level 35cm external less than canopy after pit digging, and pit digging, the periphery of canopy body is additionally provided with dewatering ditch;
(2) preparation of culture medium: the raw material of culture medium includes Pericarpium Musae 10 parts, long, thick, wide size is 30 × 0.8 × 55mm
The broken wood shavings of one-level 10 parts, long, thick, wide size is the broken wood shavings of 4 × 0.11 × 0.16mm bis-grades 8 parts, Folium Crataegi 8 parts, mutually
Flower rice grass 10 parts, Shell of Water Chestnut 12 parts, the seedpod of the lotus 9 parts, 7 parts of soy sauce waste residue, precipitated calcium carbonate 5 parts, Klinit dregs 6 parts,
Rape flower straw 7 parts, analysis for soybean powder 4 parts, marchantia 5 parts and 3 parts of Testa Tritici;
Raw material is prepared from through following concrete steps:
A Feedstock treating: one-level, two grades of broken wood shavings are classified and are exposed to the sun under the sun 2 days;By Shell of Water Chestnut and Pericarpium Musae also in the sun
Under be exposed to the sun 4 days after respectively flour to 1.5cm particle diameter;Spartina alterniflora, the seedpod of the lotus, Folium Crataegi and ground wire are dried respectively to holding nothing
Tide sense, is then respectively crushed into 1.5cm particle diameter;By after rape flower straw under high temperature sterilizing-drying through granular mill stir after,
Obtain the solid particle that particle diameter is 0.3cm;Soy sauce waste residue is processed to salinity less than 1% through the desalinization of soil by flooding or leaching;By xylitol
Slag, through high temperature acidified, controls pH=5.5;
B fermentation process: according to weight part ratio, by the broken wood shavings of one-level after step a processes, Shell of Water Chestnut, Pericarpium Musae, mutually
Hua meter Cao, the seedpod of the lotus, Folium Crataegi, rape flower straw, soy sauce waste residue, Klinit dregs and ground wire, with Testa Tritici, precipitated calcium carbonate and
Analysis for soybean powder mix and blend, adds lime cream and water, regulates pH to 6, controls moisture 65%, obtains fermentation material, will send out
Ferment material build heap fermentation 22h, fermentation temperature 45 DEG C, the most available;
C feeds: two grades of broken wood shavings after processing in step a use distilled water regulation moisture to be 55%, are then charged into cultivating
In bag, adjust two grades of wood shavings formation hollow forms and be close to outside the internal layer of culture bag, sending out after the most again step b having been fermented
Ferment material is filled in hollow position, makes fermentation material be wrapped in two grades of broken wood shavings, and then sealing obtains packed compost;
D sterilizing: by packed compost steam sterilization 14h at 100 DEG C, i.e. prepare Auricularia polytricha (Mout) Sacc. culture medium;
(3) culture medium inoculated and cultivation management: " one " font is followed closely in the inoculation being inoculated with Auricularia polytricha (Mout) Sacc. strain and is inoculated in cultivation
On base bacterium bag, two adjacent plant nail between be spaced apart the 1/3 of culture medium bacterium bag length, then by postvaccinal culture medium bacterium bag
" 1 " font that up esting becomes the number of plies to be 8 layers, and between neighbouring culture medium bacterium bag inoculation nail in triangle disposition arrange
Cloth, re-uses in the culture medium bacterium bag after esting is fixed on canopy body by some fixed mounts and under the film layer that shelters from heat or light;Then control to plant
Humidity in training booth is 90%, and temperature is 18 DEG C, and by every for the film that shelters from heat or light of canopy body surrounding after covering with mycelia in culture medium bacterium bag
It rolls period in the 10 o'clock to 12 o'clock morning in, and remaining time puts down covering, until stopping after Auricularia polytricha (Mout) Sacc. fruiting;Go out at Auricularia polytricha (Mout) Sacc.
The black film covered on the roofing of Yang Peng was rolled after 4 days by mushroom;When after Auricularia polytricha (Mout) Sacc. fruiting 15 days by the foam sheet dismounting in sun canopy;
When black film canopy body surrounding covered after Auricularia polytricha (Mout) Sacc. fruiting 12 days is period in the 3 o'clock to 5 o'clock afternoon in 10 o'clock to 12 o'clock every morning and
Rolling, remaining time puts down covering, and so circulation is until Auricularia polytricha (Mout) Sacc. sporophore fruiting is gathered.
Comparative example:
Culture medium prescription is: wood flour 78%, Testa oryzae (or Testa Tritici) 19%, sugar 1%, Gypsum Fibrosum 1%, carbamide 0.5%, calcium superphosphate
0.5%;In being 65% according to routine mixing control moisture content, pH value is 6.5, steam sterilization 28h at 100 DEG C, i.e. prepares and plants
Training substrate.Cultivation booth is common booth, and cultivation stacking mode is same as in Example 1, and regulating environmental humidity is 70-90%, temperature
Degree is less than 25 degrees Celsius, and every afternoon 4, with rear venting 2 hours, keeps dark condition until fruiting, and after fruiting, every day protects
Hold and see that light rate is 50-60%, 3-5 hour every day.
(wherein the specification of culture bag is cultivation matrix embodiment 1-3, comparative example prepared: 17cm × 33cm × 0.005
Cm, every formula 30 bags, if 3 times are repeated, every sacked material closes dry weight 400g) move in time after inoculation and send out a bacterium room, see after inoculation
Examine mycelium growth vigor, record mycelia purseful time, single bag of average product (the yield summation of first three tide is calculated as yield), calculation biology
Rate (biological efficiency=every bag of fresh mushroom production/every bag of siccative weight × 100%), sickness rate, it is shown in Table 1;To the son cultivated
Entity carries out the constituent contents such as phosphorus potassium and content of beary metal measures, and is shown in Table 2;To pluck Auricularia polytricha (Mout) Sacc. carry out auricle color and its
The situation of the impact of his character carries out record, is shown in Table 3.
Table 1 is to Auricularia polytricha (Mout) Sacc. upgrowth situation table
Project |
Mycelium growth vigor |
Mycelia purseful time (d) |
Single bag of average product (g) |
Rate biology (%) |
Embodiment 1 |
Mycelial growth is vigorous |
45±1 |
325±2 |
81.25 |
Embodiment 2 |
Mycelial growth is vigorous |
42±1 |
333±2 |
83.25 |
Embodiment 3 |
Mycelial growth is vigorous |
40±1 |
352±2 |
88 |
Comparative example |
Mycelial growth is general |
65±1 |
225±2 |
56.0 |
From the foregoing, it will be observed that the culture medium using the present invention to prepare compares comparative example with the method for cultivation management, mycelium growth vigor is more vigorous,
Mycelia purseful time advance more than two weeks, single bag of average product compares increase more than 100g, and rate was compared and improve at least biology
More than 25%, illustrate to use the culture medium of the present invention, vaccination ways, the cultivation booth combining the application and way to manage, to hair wood
The upgrowth situation of ear has greatly improved.
Table 2 planting material is on the impact of element different in Auricularia polytricha (Mout) Sacc. sporophore
Project |
Embodiment 1 |
Embodiment 2 |
Embodiment 3 |
Comparative example |
P (%) |
0.28 |
0.30 |
0.41 |
0.2 |
K (%) |
1.01 |
1.14 |
1.19 |
0.8 |
Na(mg/kg) |
1254 |
1274 |
1302 |
598 |
Ca(mg/kg) |
1150 |
1168 |
1224 |
984 |
Mg(mg/kg) |
2011 |
2018 |
2208 |
1140 |
Fe(mg/kg) |
110 |
118 |
125 |
102 |
Zn(mg/kg) |
14.7 |
15.0 |
16.1 |
10.1 |
As(mg/kg) |
0.06 |
0.06 |
0.06 |
0.05 |
Hg(mg/kg) |
0.03 |
0.02 |
0.02 |
0.03 |
Pb(mg/kg) |
< 0.5 |
< 0.5 |
< 0.5 |
< 0.5 |
Cd(mg/kg) |
< 0.1 |
< 0.1 |
< 0.1 |
< 0.1 |
As seen from the above table, the sporophore that the culture medium using the present invention to prepare is plucked, phosphorus, potassium, sodium, calcium, magnesium, zinc and manganese contain
Measure higher than the content of matched group;According to " People's Republic of China's agricultural industry criteria " (pollution-free food edible fungi NY/T749
-2012), fruit body of edible fungi dry product heavy metal standard: Pb < 2.0mg/kg, Cd < 1.0mg/kg, As < 1.0
Mg/kg, Hg < 0.2mg/kg, as shown in Table 2, in Auricularia polytricha (Mout) Sacc. sporophore, four heavy metal species content all meet green food standard.
Each embodiment of table 3 is to auricle color and the information slip of other character thereof
Project |
Fresh ear color |
Color of the trichomes |
Fine hair quantity |
Ear edge form |
Outside of belly rib back |
Embodiment 1 |
Scarlet brown |
The colour of loess |
The closeest |
Neatly |
Few |
Embodiment 2 |
Scarlet brown |
The colour of loess |
The closeest |
Neatly |
Few |
Embodiment 3 |
Scarlet brown |
The colour of loess |
The closeest |
Neatly |
Few |
Comparative example |
Bronzing |
Pale yellow |
Many |
Wave |
More |
As shown in Table 3, using cultivation matrix and the method for the present invention, the color of sporophore auricle is scarlet;In the application
Ear edge form, fine hair quantity, outside of belly rib back are better than comparative example.
Described above is the detailed description for the preferable possible embodiments of the present invention, but embodiment is not limited to the special of the present invention
Profit application range, the equal change completed under the technical spirit suggested by all present invention or modification change, the present invention all should be belonged to
Contained the scope of the claims.