CN105948909A - Saline-alkaline land improvement fertilizer containing fishskin polypeptide and preparation method thereof - Google Patents
Saline-alkaline land improvement fertilizer containing fishskin polypeptide and preparation method thereof Download PDFInfo
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- CN105948909A CN105948909A CN201610294054.2A CN201610294054A CN105948909A CN 105948909 A CN105948909 A CN 105948909A CN 201610294054 A CN201610294054 A CN 201610294054A CN 105948909 A CN105948909 A CN 105948909A
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- fishskin polypeptide
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The invention provides a saline-alkaline land improvement fertilizer containing fishskin polypeptide. The fertilizer comprises the following raw materials: potassium fulvate, fermented brewer's grains, peat, cinnamic acid, fishskin polypeptide, humus, 2-camphene, butylated urea formaldehyde resin powder, Alternanthera philoxeroides plant, diatomite, Siraitia grosvenorii sugar, pseudomonas fluorescens, and Halomonas halophiles. The invention also provides a preparation method of the fertilizer. The saline-alkaline land improvement fertilizer prepared according to the invention has an effective living bacteria number of 0.7-0.73billion cfu/g, a total nitrogen content of 4.0-4.2wt%, a total phosphorus content of 2.2-2.4wt%, a total potassium content of 3.5-3.9wt%, an organic matter content (on a dry basis) of 48.2-48.7wt%, a water content of 20.4-20.6wt%, and pH of 5.54-5.63, and a particle size (1-3mm) up to 98.7-98.9%.
Description
Technical field
The present invention relates to a kind of alkaline land improving fertilizer containing fishskin polypeptide and preparation method thereof, belong to alkaline land improving fertile
Technical field.
Background technology
Various saline-alkali soil are all to be formed under certain natural conditions, and its essence formed is mainly various ease of solubility salt
Class makees redistributing of horizontal direction and vertical direction on ground, so that the upper soll layer that salinity is in collection salt area builds up
Getting up, the basic reason that saline-alkali soil is formed is that water regime is bad, so at the improvement initial stage, emphasis should be placed on and improve soil
Above water regime.Typically it is carried out in several steps, first salt discharge, the desalinization of soil by flooding or leaching, reduction soil salt content;Plant planting of Salt And Alkali Tolerance again
Thing, fertilizing soil;Last long-term cropping.Concrete ameliorative measure is: draining, irrigates the desalinization of soil by flooding or leaching, warps improvement, rice cultivation, training
Fertile improvement, levels land and chemical modifying.
Conventional chemical modifying method is that alkaline earth, alkali soil, soda-saline soil are applied the modifying agent such as Gypsum Fibrosum, Alumen preparata, reduces
Or elimination soil alkali divides, improve soil physico-chemical property.
Existing alkaline land improving fertilizer has the disadvantage that
(1) there is the phenomenons such as effective bacterial content is low, miscellaneous bacteria exceeds standard, also some producer adds an inappropriate amount of chemical fertilizer in bio-feritlizer
Or other additive, make that the nitrogen of chemical fertilizer in fertilizer, phosphorus, potassium content are too high causes effective bacterium dead, and lose the effect of bio-fertilizer;
(2) improve the weak effect in salt-soda soil, it is impossible to effectively reduce the pH value of soil simultaneously, reduce the basicity of soil, reduce
The salinity of soil, improves soil with organic matter content, improves water capacity, improves the water-stable granule of 5-7mm in soil
Mechanical stability, improves retention capacity of soil;Increase Nitrogen In Soils phosphorus potassium content, reduce Cadmium in Soil content;
(3) potato planting in salt-soda soil, the survival rate of Rhizoma Solani tuber osi is low, and the per mu yield of Rhizoma Solani tuber osi is low;
(4) potato planting in salt-soda soil, to the weeds in Rhizoma Solani tuber osi field without prevention effect;
(5) potato planting in salt-soda soil, to the late blight of potato, balck shank without prevention or prevention effect.
Summary of the invention
For solving technical problem present in prior art, the present invention provides a kind of alkaline land improving containing fishskin polypeptide fertile
Material and preparation method thereof, to realize following goal of the invention:
(1) the alkaline land improving fertilizer that prepared by the present invention, fertilizer prepared by the present invention, living bacteria count amount is 7.0-7.3 hundred million
Cfu/g, total nitrogen content is 4.0-4.2wt%, and total phosphorus content is 2.2-2.4wt%, and total potassium content is 3.5-3.9wt%, organic
(in terms of butt), as 48.2-48.7wt%, moisture content 20.4-20.6wt%, pH are 5.54-5.63, and granularity (1-3mm) is 98.7-
98.9%, miscellaneous bacteria rate is 2.7-3.0%, and in terms of butt, total arsenic content≤1.5mg/kg, total cadmium content≤0.6mg/kg, total lead contains
Amount≤5.1mg/kg, total chrome content≤8mg/kg, total mercury content≤0.3mg/kg.
(2) the alkaline land improving fertilizer that prepared by the present invention, for alkaline land improving, reduces the pH value of soil, reduces soil
Basicity, reduce the salinity of soil, improve soil with organic matter content, improve water capacity, improve 5-7mm in soil
Water-stable granule mechanical stability, improve retention capacity of soil;Increase Nitrogen In Soils phosphorus potassium content, reduce Cadmium in Soil and contain
Amount.
(3) fertilizer of the present invention, for potato planting, survival rate 95.7-97.6% of Rhizoma Solani tuber osi, the per mu yield of Rhizoma Solani tuber osi
Amount reaches 2300-2400 kg/acre, and in Rhizoma Solani tuber osi, protein and dietary fiber content also significantly improve.
(4) fertilizer of the present invention, for potato planting, reaches 74.6-to the preventive effect of the weeds-barnyard grass in Rhizoma Solani tuber osi field
78.6%, the preventive effect of weeds-Herba Setariae Viridis is reached 81.4-85.7%.
(5) fertilizer of the present invention, for potato planting, has an effect reducing Rhizoma Solani tuber osi disease, the late blight of potato, black
The sickness rate of shin disease is low.
For solving above-mentioned technical problem, the technical scheme that the present invention takes is as follows:
A kind of alkaline land improving fertilizer containing fishskin polypeptide, described fertilizer, raw material includes potassium fulvate, distiller's grains of beer fermented product, mud
Charcoal, cinnamic acid, fishskin polypeptide, humus, 2-bornene, butyl ether urea-formaldehyde resin powder, water peanut plant, kieselguhr, momordica grosvenori sugar,
Pseudomonas fluorescens, Halomonas bite salt bacterium.
The following is and the further of technique scheme is improved:
Described fertilizer, in parts by weight, including following raw material components: potassium fulvate 10-15 part, distiller's grains of beer fermented product 20-25 part,
Peat 11-16 part, cinnamic acid 8-13 part, fishskin polypeptide 5-9 part, humus 17-23 part, 2-bornene 6-9 part, fourth etherified urea aldehyde tree
Cosmetics 12-15 part, water peanut plant 4-7 part, kieselguhr 9-15 part, momordica grosvenori sugar 5-10 part, pseudomonas fluorescens 1-4 part, salt
Zygosaccharomyces bites salt bacterium 2-6 part.
Described humus, the content of organic matter is up to 35.5%.
Described kieselguhr, dioxide-containing silica is 99%, and iron oxide content is 0.8%, loose thickness≤0.35g/cm3, than
Surface area is 0.38m2/ g, screen over-size≤0.15%, loss on ignition≤0.1%;Particle diameter is 1-2mm.
Described distiller's grains of beer fermented product, cellulase activity is 1500u/g, and the enzyme of xylanase is lived as 1200u/g, beta glucan
The enzyme of enzyme is alive is 200u/g, and it is 600u/g that the enzyme of pectase is lived, and it is 300u/g that diastatic enzyme is lived, and the enzyme work of protease is
200u/g;Crude protein content >=20%;Moisture≤10%, ash≤10%;Saccharomyces cerevisiae bacterial content is 5 × 108cfu/g.
Described pseudomonas fluorescens, for mycopowder, bacterial content is 1 × 109cfu/g;Described Halomonas bites salt bacterium, for bacterium
Powder, bacterial content is 1.5 × 109cfu/g。
The preparation method of a kind of alkaline land improving fertilizer containing fishskin polypeptide, described method includes the preparation of fishskin polypeptide;
The preparation of described fishskin polypeptide, including fish skin pre-treatment, first stage enzymolysis,
Second stage enzymolysis.
Described fish skin pre-treatment, soaks 1d defat with the n-butyl alcohol that volume fraction is 10% of solid-liquid ratio 1:8, then with 4%
Sodium-chloride water solution soaks 20 minutes, removes the dope on fish skin surface, adds the sodium bicarbonate of 2% after cleaning.
Described first stage enzymolysis, hydrolysis temperature is 85 DEG C, adds the fire resistant alpha-diastase of 0.03wt%, 0.04 wt %
High temperature resistant lipase, the thermostable proteinase of 0.1wt%, insulation enzymolysis 3 hours.
Described second stage enzymolysis, is cooled to 50 DEG C, regulates pH6.5, according to 2.2% addition local flavor albumen of substrate weight
Enzyme, papain, bromelain, neutral protease, mass ratio is 5:2:4:3, insulation enzymolysis 2 hours.
Compared with prior art, the invention have the benefit that
(1) the alkaline land improving fertilizer that prepared by the present invention, living bacteria count amount is 7.0-7.3 hundred million cfu/g, and total nitrogen content is 4.0-
4.2wt%, total phosphorus content is 2.2-2.4wt%, and total potassium content is 3.5-3.9wt%, and organic (in terms of butt) is as 48.2-
48.7wt%, moisture content 20.4-20.6wt%, pH are 5.54-5.63, and granularity (1-3mm) is 98.7-98.9%, and miscellaneous bacteria rate is 2.7-
3.0%, in terms of butt, total arsenic content≤1.5mg/kg, total cadmium content≤0.6mg/kg, total lead content≤5.1mg/kg, total chromium contains
Amount≤8mg/kg, total mercury content≤0.3mg/kg.
(2) the alkaline land improving fertilizer that prepared by the present invention, for alkaline land improving, after 6 months, measures soil root system, soil
The pH value of earth is 6.8-7.2, and the basicity of soil is 3.0-4.2%, and the salinity of soil is 0.15-0.20%, organic in soil
Matter content is 11.5-12.8g/kg, and water capacity is 24-31%, and in soil, the water-stable granule mechanical stability of 5-7mm carries
High 60.4-65.5%, retention capacity of soil improves 59.8-62.8%;The total nitrogen content of soil is 0.103-0.12%, has in soil
Effect phosphorus content is 42.6-50.3ppm, and in soil, effective potassium content is 214-230ppm;Cadmium in Soil content is 1.0-1.35
mg/kg。
(3) fertilizer of the present invention, for potato planting, the survival rate of Rhizoma Solani tuber osi is 95.7-97.6%, the per mu yield of Rhizoma Solani tuber osi
Amount is for 2300-2400 kg/acre, and in Rhizoma Solani tuber osi, protein and dietary fiber content also significantly improve, when water content is 79.8g/
During 100g, protein content is 2.61-2.67g/100g, and dietary fiber content is 0.91-0.99g/100g.
(4) fertilizer of the present invention, for potato planting, the preventive effect to the weeds-barnyard grass in Rhizoma Solani tuber osi field is 74.6-
78.6%, the preventive effect to weeds-Herba Setariae Viridis is 81.4-85.7%.
(5) fertilizer of the present invention, for potato planting, has the effect reducing Rhizoma Solani tuber osi disease, the late blight of potato
Sickness rate is 6.2-8.6%;The sickness rate of balck shank is 8.1-12.7%.
Comparison: not using improving fertilizer, directly plant Rhizoma Solani tuber osi in salt-soda soil, the survival rate of Rhizoma Solani tuber osi is only 20.4%, Ma Ling
The annual production of potato is 400 kgs/acre, and when water content is 79.8g/100g, protein content is 2.4g/100g, dietary fiber
Content is 0.84g/100g;The sickness rate of the late blight of potato is 75%;The sickness rate of balck shank is 85%;And weeds are more.
Detailed description of the invention
Hereinafter the preferred embodiments of the present invention are illustrated, it will be appreciated that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
The embodiment 1 one kinds alkaline land improving fertilizer containing fishskin polypeptide
In parts by weight, including following raw material components:
Potassium fulvate 10 parts, distiller's grains of beer fermented product 20 parts, 11 parts of peat, cinnamic acid 8 parts, fishskin polypeptide 5 parts, 17 parts of humus,
2-bornene 6 parts, butyl ether urea-formaldehyde resin powder 12 parts, water peanut plant 4 parts, 9 parts of kieselguhr, momordica grosvenori sugar 5 parts, Pseudomonas
Bacterium 1 part, Halomonas bite salt bacterium 2 parts.
Described humus, the content of organic matter is up to 35.5%;
Described kieselguhr, dioxide-containing silica is 99%, and iron oxide content is 0.8%, loose thickness≤0.35g/cm3, specific surface
Amass as 0.38m2/g, screen over-size≤0.15%, loss on ignition≤0.1%;Particle diameter is 1-2mm;
Described distiller's grains of beer fermented product, cellulase activity is 1500u/g, and it is 1200u/g that the enzyme of xylanase is lived, 1,4 beta-glucanase
Enzyme is alive is 200u/g, and it is 600u/g that the enzyme of pectase is lived, and it is 300u/g that diastatic enzyme is lived, and the enzyme of protease is alive for 200u/g;
Crude protein content >=20%;Moisture≤10%, ash≤10%;Saccharomyces cerevisiae bacterial content is 5 × 108cfu/g。
Described pseudomonas fluorescens (Pseudomonas fluorescens), culture presevation number is ACCC 01014;
Described pseudomonas fluorescens, for mycopowder, bacterial content is 1 × 109cfu/g;
Described Halomonas bites salt bacterium, and for mycopowder, bacterial content is 1.5 × 109cfu/g。
Described pseudomonas fluorescens, Halomonas bite salt bacterium, derive from commercially available.
The embodiment 2 one kinds alkaline land improving fertilizer containing fishskin polypeptide
Use each raw material described in embodiment 1, only change the proportioning of each raw material, change into:
In parts by weight, including following raw material components:
Potassium fulvate 12 parts, distiller's grains of beer fermented product 22 parts, 14 parts of peat, cinnamic acid 12 parts, fishskin polypeptide 7 parts, 20 parts of humus,
2-bornene 8 parts, butyl ether urea-formaldehyde resin powder 13 parts, water peanut plant 5 parts, 13 parts of kieselguhr, momordica grosvenori sugar 7 parts, fluorescence vacation list
Born of the same parents bacterium 2 parts, Halomonas bite salt bacterium 5 parts.
The embodiment 3 one kinds alkaline land improving fertilizer containing fishskin polypeptide
Use each raw material described in embodiment 1, only change the proportioning of each raw material, change into:
In parts by weight, including following raw material components:
Potassium fulvate 15 parts, distiller's grains of beer fermented product 25 parts, 16 parts of peat, cinnamic acid 13 parts, fishskin polypeptide 9 parts, 23 parts of humus,
2-bornene 9 parts, butyl ether urea-formaldehyde resin powder 15 parts, water peanut plant 7 parts, 15 parts of kieselguhr, momordica grosvenori sugar 10 parts, fluorescence vacation list
Born of the same parents bacterium 4 parts, Halomonas bite salt bacterium 6 parts.
The preparation method of the embodiment 4 one kinds alkaline land improving fertilizer containing fishskin polypeptide
Comprise the following steps:
Step one, the acidification of water peanut plant
The pre-treatment of vegetable material: the water peanut plant material (whole strain material) that will cradle or pluck, by collecting, clean, drying in the air
Shine, crush, be the saline soak of 2.0-2.4%, sterilizing, formation acidifying raw material by concentration;
The acidifying of vegetable material: put into acidifying raw material in acidification vessel, add and be acidified microbial inoculum, add water, acidifying raw material and water
Mass ratio is 1:16, floods acidifying raw material, adds the acidifying microbial inoculum of 1.5-1.8wt%, covers container, sealing with plastic foil, protects
Holding the temperature in acidification vessel and be 15-16 DEG C, temperature retention time is 35 days, and pressure is 0.04-0.06MPa, is dried by acidifying raw material,
Form organic acidulants;
Described acidifying microbial inoculum is liquid bacterial agent, including bacillus acidophilus, acetic acid bacteria, lactobacillus casei, and wherein bacillus acidophilus, vinegar
Acid bacterium, the bacteria concentration of lactobacillus casei are respectively 1.3 × 108cfu/ml、1.6×108cfu/ml、1.7×108cfu/ml。
Described microbial inoculum derives from market and buys.
Step 2, the preparation of fishskin polypeptide
(1) fish skin pre-treatment
The fish skin of frost is removed, thaws, fall surface impurity by clean water, be 10% just by the volume fraction of solid-liquid ratio 1:8
Butanol soaks 1d defat, then soaks 20 minutes with the sodium-chloride water solution of 4%, removes the dope on fish skin surface, adds after cleaning again
Enter the sodium bicarbonate of 2%, stirring, remove lipid material, filtration and washing.
(2) collagen liquid is prepared
The fish skin of pre-treatment is placed in steaming and decocting under high pressure machine, after adding water, steaming and decocting 60 minutes by the volume ratio of 1:12, stops adding
Heat, filters and removes insoluble matter, obtain collagen liquid;
(3) first stage enzymolysis
Keep hydrolysis temperature be 85 DEG C, toward collagen liquid prepared by step (2) in, interpolation 0.03wt% fire resistant alpha-diastase,
The high temperature resistant lipase of 0.04 wt %, the thermostable proteinase of 0.1wt%, insulation enzymolysis 3 hours;
(4) second stage enzymolysis
It is cooled to 50 DEG C, regulates pH6.5, according to 2.2% addition flavor protease, papain, bromelain of substrate weight
Enzyme, neutral protease (mass ratio is 5:2:4:3), speed of agitator is 800r/min, and is incubated enzymolysis 2 hours;Period is carried out
Stirring, speed of agitator is 800r/min, is warmed up to 100 DEG C of enzyme denaturing 10 minutes, filters, obtains filtrate, concentrates, is dried, obtains polypeptide powder;
In described polypeptide powder, the polypeptide proportion of below molecular weight 3000u is 60-65%.
Step 3, weighing
According to formula, weigh each raw material components;
Step 4, mixing
By the water peanut plant after acidification, and other raw material mix homogeneously, be dried to moisture content be 13-15%;It is crushed to
1-3mm, obtains fertilizer of the present invention.
Embodiment 5 applies effect
Fertilizer prepared by the present invention, living bacteria count amount is 7.0-7.3 hundred million cfu/g, and total nitrogen content is 4.0-4.2wt%, and total phosphorus contains
Amount is for 2.2-2.4wt%, and total potassium content is 3.5-3.9wt%, organic (in terms of butt) as 48.2-48.7wt%, moisture content
20.4-20.6wt%, pH are 5.54-5.63, and granularity (1-3mm) is 98.7-98.9%, and miscellaneous bacteria rate is 2.7-3.0%, in terms of butt,
Total arsenic content≤1.5mg/kg, total cadmium content≤0.6mg/kg, total lead content≤5.1mg/kg, total chrome content≤8mg/kg, always
Mercury content≤0.3mg/kg.
Fertilizer prepared by the present invention, is applied to salt-soda soil, and consumption is 1500 pounds/acre, within after fertilising every 15 days, waters 1 time, 2
Plant Rhizoma Solani tuber osi after Yue, plant according to routine techniques;
Alkaline land improving fertilizer of the present invention, for alkaline land improving, after 6 months, measures soil root system, and the pH value of soil is 6.8-
7.2, the basicity of soil is 3.0-4.2%, and the salinity of soil is 0.15-0.20%, and soil with organic matter content is 11.5-
12.8g/kg, water capacity is 24-31%, and in soil, the water-stable granule mechanical stability of 5-7mm improves 60.4-65.5%,
Retention capacity of soil improves 59.8-62.8%;The total nitrogen content of soil is 0.103-0.12%, and in soil, available phosphorus content is
42.6-50.3ppm, in soil, effective potassium content is 214-230ppm, and Cadmium in Soil content is 1.0-1.35 mg/kg;
Index before alkaline land improving: the pH value of soil is 8.7-8.9, the basicity of soil is 14-15%, the salinity of soil
For 2.2-2.4%, soil with organic matter content is 4.3-4.5g/kg, and water capacity is 7-8%, and the total nitrogen content of soil is
0.02%, in soil, available phosphorus content is 8ppm, and in soil, effective potassium content is 70ppm, and in soil, cadmium content is 4mg/kg.
Index after table 1 soil improvement
Alkaline land improving of the present invention is fertile, for potato planting,
The survival rate of Rhizoma Solani tuber osi is 95.7-97.6%, and the per mu yield of Rhizoma Solani tuber osi is 2300-2400 kg/acre, albumen in Rhizoma Solani tuber osi
Matter and dietary fiber content also significantly improve, and when water content is 79.8g/100g, protein content is 2.61-2.67g/
100g, dietary fiber content is 0.91-0.99g/100g;Fertilizer of the present invention preventing for the weeds-barnyard grass in Rhizoma Solani tuber osi field
Effect is 74.6-78.6%;Fertilizer of the present invention is 81.4-85.7% for the preventive effect of the weeds-Herba Setariae Viridis in Rhizoma Solani tuber osi field;Ma Ling
The sickness rate of potato late blight reaches 6.2-8.6%;The sickness rate of balck shank is 8.1-12.7%;
Comparison: not using improving fertilizer, directly plant Rhizoma Solani tuber osi, the survival rate of Rhizoma Solani tuber osi is only 20.4%, and the annual production of Rhizoma Solani tuber osi is
400 kgs/acre, when water content is 79.8g/100g, protein content is 2.4g/100g, and dietary fiber content is 0.84g/
100g;The sickness rate of the late blight of potato is 75%;The sickness rate of balck shank is 85%;And weeds are more.
The index of correlation of table 2 Rhizoma Solani tuber osi
Unless specifically indicated, ratio of the present invention is mass ratio, described percent, is mass percent.
Finally it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention,
Although being described in detail the present invention with reference to previous embodiment, for a person skilled in the art, it still may be used
So that the technical scheme described in foregoing embodiments to be modified, or wherein portion of techniques feature is carried out equivalent.
All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included in the present invention's
Within protection domain.
Claims (10)
1. the alkaline land improving fertilizer containing fishskin polypeptide, it is characterised in that: described fertilizer, raw material includes potassium fulvate, beer
Vinasse fermentation thing, peat, cinnamic acid, fishskin polypeptide, humus, 2-bornene, butyl ether urea-formaldehyde resin powder, water peanut plant, silicon
Diatomaceous earth, momordica grosvenori sugar, pseudomonas fluorescens, Halomonas bite salt bacterium.
A kind of alkaline land improving fertilizer containing fishskin polypeptide the most according to claim 1, it is characterised in that: described fertilizer,
In parts by weight, including following raw material components: potassium fulvate 10-15 part, distiller's grains of beer fermented product 20-25 part, peat 11-16 part,
Cinnamic acid 8-13 part, fishskin polypeptide 5-9 part, humus 17-23 part, 2-bornene 6-9 part, butyl ether urea-formaldehyde resin powder 12-15 part,
Water peanut plant 4-7 part, kieselguhr 9-15 part, momordica grosvenori sugar 5-10 part, pseudomonas fluorescens 1-4 part, Halomonas bite salt
Bacterium 2-6 part.
A kind of alkaline land improving fertilizer containing fishskin polypeptide the most according to claim 1, it is characterised in that: described humic
Matter, the content of organic matter is up to 35.5%.
A kind of alkaline land improving fertilizer containing fishskin polypeptide the most according to claim 1, it is characterised in that: described diatom
Soil, dioxide-containing silica is 99%, and iron oxide content is 0.8%, loose thickness≤0.35g/cm3, specific surface area is 0.38m2/ g,
Screen over-size≤0.15%, loss on ignition≤0.1%;Particle diameter is 1-2mm.
A kind of alkaline land improving fertilizer containing fishskin polypeptide the most according to claim 1, it is characterised in that: described distiller's grains of beer
Fermented product, cellulase activity is 1500u/g, and it is 1200u/g that the enzyme of xylanase is lived, and it is 200u/g that the enzyme of 1,4 beta-glucanase is lived,
It is 600u/g that the enzyme of pectase is lived, and it is 300u/g that diastatic enzyme is lived, and the enzyme of protease is lived as 200u/g;Crude protein content >=
20%;Moisture≤10%, ash≤10%;Saccharomyces cerevisiae bacterial content is 5 × 108cfu/g.
A kind of alkaline land improving fertilizer containing fishskin polypeptide the most according to claim 1, it is characterised in that: described fluorescence is false
Zymomonas mobilis, for mycopowder, bacterial content is 1 × 109cfu/g;Described Halomonas bites salt bacterium, and for mycopowder, bacterial content is 1.5 ×
109cfu/g。
7. the preparation method of the alkaline land improving fertilizer containing fishskin polypeptide, it is characterised in that: described method includes that fish skin is many
The preparation of peptide;The preparation of described fishskin polypeptide, including fish skin pre-treatment, first stage enzymolysis, second stage enzymolysis.
The preparation method of a kind of alkaline land improving fertilizer containing fishskin polypeptide the most according to claim 7, it is characterised in that:
Described fish skin pre-treatment, soaks 1d defat, then the sodium chloride water with 4% with the n-butyl alcohol that volume fraction is 10% of solid-liquid ratio 1:8
Solution soaking 20 minutes, removes the dope on fish skin surface, adds the sodium bicarbonate of 2% after cleaning.
The preparation method of a kind of alkaline land improving fertilizer containing fishskin polypeptide the most according to claim 7, it is characterised in that:
Described first stage enzymolysis, hydrolysis temperature is 85 DEG C, adds the fire resistant alpha-diastase of 0.03wt%, the high temperature grease of 0.04 wt %
Fat enzyme, the thermostable proteinase of 0.1wt%, insulation enzymolysis 3 hours.
The preparation method of a kind of alkaline land improving fertilizer containing fishskin polypeptide the most according to claim 7, its feature exists
In: described second stage enzymolysis, it is cooled to 50 DEG C, regulates pH6.5, according to 2.2% addition flavor protease, wood of substrate weight
Melon protease, bromelain, neutral protease, mass ratio is 5:2:4:3, insulation enzymolysis 2 hours.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108395351A (en) * | 2017-02-08 | 2018-08-14 | 山东中创亿丰肥料集团有限公司 | A kind of biological bacteria fish peptide fertilizer, preparation method and its production equipment special |
CN111410580A (en) * | 2020-03-31 | 2020-07-14 | 农业农村部规划设计研究院 | Method for preparing peptide calcium biostimulant from waste containing colloidal protein |
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2016
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108395351A (en) * | 2017-02-08 | 2018-08-14 | 山东中创亿丰肥料集团有限公司 | A kind of biological bacteria fish peptide fertilizer, preparation method and its production equipment special |
CN111410580A (en) * | 2020-03-31 | 2020-07-14 | 农业农村部规划设计研究院 | Method for preparing peptide calcium biostimulant from waste containing colloidal protein |
CN111410580B (en) * | 2020-03-31 | 2022-08-12 | 农业农村部规划设计研究院 | Method for preparing peptide calcium biostimulant from waste containing colloidal protein |
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Application publication date: 20160921 |