CN105942053A - Immunity-regulating maca functional drink and preparation method thereof - Google Patents
Immunity-regulating maca functional drink and preparation method thereof Download PDFInfo
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- CN105942053A CN105942053A CN201610271865.0A CN201610271865A CN105942053A CN 105942053 A CN105942053 A CN 105942053A CN 201610271865 A CN201610271865 A CN 201610271865A CN 105942053 A CN105942053 A CN 105942053A
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- 235000000421 Lepidium meyenii Nutrition 0.000 title claims abstract description 50
- 235000012902 lepidium meyenii Nutrition 0.000 title claims abstract description 50
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 51
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims abstract description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 42
- 230000036039 immunity Effects 0.000 claims abstract description 30
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 229960003080 taurine Drugs 0.000 claims abstract description 23
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 19
- 238000005374 membrane filtration Methods 0.000 claims abstract description 8
- 150000004676 glycans Chemical class 0.000 claims abstract 3
- 229920001282 polysaccharide Polymers 0.000 claims abstract 3
- 239000005017 polysaccharide Substances 0.000 claims abstract 3
- 239000000203 mixture Substances 0.000 claims description 39
- 239000002253 acid Substances 0.000 claims description 31
- 235000013361 beverage Nutrition 0.000 claims description 27
- 238000003756 stirring Methods 0.000 claims description 26
- 230000001105 regulatory effect Effects 0.000 claims description 24
- 229930006000 Sucrose Natural products 0.000 claims description 23
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 23
- 239000005720 sucrose Substances 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 21
- 239000000725 suspension Substances 0.000 claims description 17
- 239000003381 stabilizer Substances 0.000 claims description 16
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 15
- 239000000706 filtrate Substances 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 6
- 239000008187 granular material Substances 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 235000021433 fructose syrup Nutrition 0.000 claims description 3
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 2
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 235000009508 confectionery Nutrition 0.000 claims description 2
- 239000001630 malic acid Substances 0.000 claims description 2
- 235000011090 malic acid Nutrition 0.000 claims description 2
- 240000000111 Saccharum officinarum Species 0.000 claims 2
- 235000007201 Saccharum officinarum Nutrition 0.000 claims 2
- 244000000231 Sesamum indicum Species 0.000 claims 1
- 235000003434 Sesamum indicum Nutrition 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 12
- 238000005516 engineering process Methods 0.000 abstract description 8
- 235000015197 apple juice Nutrition 0.000 abstract 3
- 239000004615 ingredient Substances 0.000 abstract 3
- 240000008397 Ganoderma lucidum Species 0.000 abstract 2
- 235000001637 Ganoderma lucidum Nutrition 0.000 abstract 2
- 239000000243 solution Substances 0.000 description 29
- 239000003814 drug Substances 0.000 description 25
- 238000012360 testing method Methods 0.000 description 22
- 238000002474 experimental method Methods 0.000 description 14
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 10
- 241001465754 Metazoa Species 0.000 description 10
- 229960004397 cyclophosphamide Drugs 0.000 description 10
- 239000012153 distilled water Substances 0.000 description 10
- 210000002966 serum Anatomy 0.000 description 10
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 description 9
- 241000699670 Mus sp. Species 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 230000037396 body weight Effects 0.000 description 7
- 241000287828 Gallus gallus Species 0.000 description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
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- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 108010006464 Hemolysin Proteins Proteins 0.000 description 4
- 238000012449 Kunming mouse Methods 0.000 description 4
- 235000013353 coffee beverage Nutrition 0.000 description 4
- 210000003743 erythrocyte Anatomy 0.000 description 4
- 230000002496 gastric effect Effects 0.000 description 4
- 239000003228 hemolysin Substances 0.000 description 4
- 230000001900 immune effect Effects 0.000 description 4
- 241000700199 Cavia porcellus Species 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000004821 distillation Methods 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- 230000003749 cleanliness Effects 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 229940084983 cyclophosphamide 50 mg Drugs 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
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- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010015719 Exsanguination Diseases 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 240000001970 Raphanus sativus var. sativus Species 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical compound CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- MPOKJOWFCMDRKP-UHFFFAOYSA-N gold;hydrate Chemical compound O.[Au] MPOKJOWFCMDRKP-UHFFFAOYSA-N 0.000 description 1
- 238000005534 hematocrit Methods 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The present invention relates to the technical field of maca drinks and particularly relates to an immunity-regulating maca functional drink and a preparation method thereof. By using maca juice, apple juice, ganoderma lucidum polysaccharides and taurine as main raw materials, the raw material ratios are defined and the raw materials are prepared into the health-care drink. An appropriate amount of the apple juice continuously improves the mouthfeel of the maca drink. By adding the ganoderma lucidum polysaccharides and taurine, the materials act together with the maca, so that the drink can improve human body immunity. By combining a used membrane filtration technology in the preparation technology steps, and defining the technology steps, the ingredients of the maca, apple juice and other raw materials do not get oxidized and browned. Besides, in the whole technology, water at a certain temperature is used to dissolve the raw material ingredients, and then the dissolved raw materials are added into material liquid to avoid the maca raw material ingredients being treated at high temperature, which effectively avoids the browning of the drink under the high temperature conditions and ensures the quality of the drink.
Description
Technical field
The present invention relates to Maca beverage technical field, especially a kind of immunity regulating type Lepidinm meyenii Walp function
Beverage and preparation method thereof.
Background technology
Maca, formal name used at school: Lepidium meyenii Walp, Spanish: Maca.Mainly go out
Originate in South America Andes normal pulse Chinese yunnan Lijing, be a kind of crucifer.Leaf
Ellipse, rhizome likeness in form turnip radish, edible, is a kind of natural food, and nutrition is rich
Rich.The hypocotyl of Maca may be in golden or faint yellow, red, purple, blueness, black
Or it is green.Flaxen most common, and shape, taste are preferably also.Maca is rich in high unit
Nutrient, has the function of nourishing and fit keeping function to human body.It is best that black Maca is acknowledged as effect
Maca, yield is few.
China has abundant Lepidinm meyenii Walp resource, and along with Lepidinm meyenii Walp efficacy performance is found, Lepidinm meyenii Walp
Range of application obtained large-scale application and popularization；And the kind of Lepidinm meyenii Walp product is the richest
Richness, such as Maca beverage, Maca wine, Maca coffee, Lepidinm meyenii Walp Chinese medicine preparation etc..
But, the health-care effect of Lepidinm meyenii Walp product of the prior art is the most poor, especially at agate
Coffee field of beverage, it requires that the mouthfeel of Maca beverage is more excellent, and health-care effect is eager to excel；But it is existing
Having in technology and as raw material prepared by Maca beverage such as Lepidinm meyenii Walp, Fructus Mali pumilae, sugar etc., it can not be complete
Embody the effects such as the enhancing immunity that Lepidinm meyenii Walp had；And traditional Maca beverage prepares work
In skill, it is improper due to processing mode, easily causes brown stain so that the quality of Maca beverage is relatively
Difference, and the less stable of the Lepidinm meyenii Walp product prepared, be easily layered, go bad, hinder agate
The development of coffee beverage.
Summary of the invention
In order to solve above-mentioned technical problem present in prior art, the present invention provides a kind of immunity
Adjustment type Maca functional drink and preparation method thereof.
It is achieved particular by techniques below scheme:
A kind of immunity regulating type Maca functional drink, material composition is calculated as Lepidinm meyenii Walp juice with mass parts
1-3 part, Sucus Mali pumilae 5-10 part, acid 0.3-0.5 part, sucrose 2-4 part, ganoderan 0.05-0.07
Part, taurine 0.04-0.06 part, suspension stabilizer 1-3 part.
Described material composition with mass parts be calculated as 2 parts of Lepidinm meyenii Walp juice, Sucus Mali pumilae 8 parts, acid 0.4 part,
Sucrose 3 parts, ganoderan 0.06 part, taurine 0.05 part, suspension stabilizer 2 parts.
Described material composition with mass parts be calculated as 3 parts of Lepidinm meyenii Walp juice, Sucus Mali pumilae 10 parts, acid 0.5 part,
Sucrose 4 parts, ganoderan 0.07 part, taurine 0.06 part, suspension stabilizer 1 part.
Described material composition, also includes appropriate essence.
Described material composition, also includes Fructus Crataegi and the mixed extracting solution of Radix Glycyrrhizae.
Described material composition, the proportioning of its Fructus Crataegi and Radix Glycyrrhizae is mixed in equal amounts, adds 10 times of weights
The water of the Fructus Crataegi of amount, at the 90-130 DEG C of extracting solution obtained after decocting and extracting 30-50min.
Described material composition, the addition of extracting solution is calculated as 1-3 part with mass parts.
Described acid is at least one in citric acid, malic acid.
Described sucrose, uses at least one in high fructose syrup, sweet sugar TR100 to substitute.
Preparation method, comprises the following steps:
(1) select fresh Lepidinm meyenii Walp root, filter after squeezing the juice, take filtrate and mix with Sucus Mali pumilae,
To feed liquid；
(2) sucrose of 1/10 weight portion is taken, after uniform to itself and suspension stabilizer dry blending,
Joining in the water that temperature is 75-85 DEG C, stirring is fully dissolved, after filtering out insoluble granule,
Join in feed liquid；
(3) residue sucrose is joined in 75-85 DEG C of water, after stirring is fully dissolved, join
In the feed liquid of step (2), add ganoderan, taurine stirs；Or add
Ganoderan, taurine, Fructus Crataegi and the mixed extracting solution of Radix Glycyrrhizae, stir, mixed
Compound material；
(4) water of acid 55-65 DEG C is fully dissolved, obtain acid solution；And acid solution is slowly added
Enter in mixed material, stir；
(5) after the material of step (4) being added essence or being added without essence, 35-45 DEG C,
Under 18-22MPa, homogenizing processes；
(6) use membrane aperture be 0.2 micron worry membrane filtration process, and will filter after filtrate
Load in vial, and be placed on 2-8 DEG C of chilling treatment, it is thus achieved that immunity regulating type Lepidinm meyenii Walp merit
Can beverage.
Compared with prior art, the technique effect of the present invention is embodied in:
By with Lepidinm meyenii Walp juice, Sucus Mali pumilae, ganoderan, taurine as primary raw material, and to it
Proportioning raw materials is defined, and is prepared into health beverage so that appropriate Fructus Mali pumilae juice is to agate
The mouthfeel of coffee beverage continues to improve, in the addition combining ganoderan, taurine so that its with
Lepidinm meyenii Walp acts on jointly so that this beverage can improve body immunity；And combine preparation technology step
Membrane filtration technique, and the restriction of processing step is used so that Lepidinm meyenii Walp, Sucus Mali pumilae etc. are former in rapid
Material composition will not occur oxidizing brown stain, and uses the water dissolution of uniform temperature in whole technique
After material composition, then add it in feed liquid, it is to avoid high-temperature process Lepidinm meyenii Walp material composition, have
The beverage brown stain avoided under hot conditions of effect, it is ensured that beverage quality.
This researcher is by defining the raw material addition sequence in preparation technology so that each raw material
After addition, act on feed liquid, and then guarantee the taste good of Maca beverage, and combine
The restriction of technological parameter, is not result in that nutrition damages, and improves the quality of Maca beverage；This
Bright operability is stronger, it is easy to popularization and application.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme is further limited
Fixed, but claimed scope is not only limited to description.
Embodiment 1
A kind of immunity regulating type Maca functional drink, material composition be in mass Lepidinm meyenii Walp juice 1kg,
Sucus Mali pumilae 5kg, acid 0.3kg, sucrose 2kg, ganoderan 0.05kg, taurine 0.04kg, outstanding
Floating stabilizer 1kg.
Preparation method, comprises the following steps:
(1) select fresh Lepidinm meyenii Walp root, filter after squeezing the juice, take filtrate and mix with Sucus Mali pumilae,
To feed liquid；
(2) take the sucrose of 1/10 weight, after uniform to itself and suspension stabilizer dry blending, add
Entering in the water that temperature is 75 DEG C, stirring is fully dissolved, and after filtering out insoluble granule, adds
In feed liquid；
(3) residue sucrose is joined in 75 DEG C of water, after stirring is fully dissolved, join step
Suddenly in the feed liquid of (2), add ganoderan, taurine stirs；Obtain mixture
Material；
(4) acid is fully dissolved with the water of 55 DEG C, obtain acid solution；And acid solution is slowly added to
In mixed material, stir；
(5) by the material of step (4), 35 DEG C, homogenizing processes under 18MPa；
(6) use membrane aperture be 0.2 micron worry membrane filtration process, and will filter after filtrate
Load in vial, and be placed on 2 DEG C of chilling treatment, it is thus achieved that immunity regulating type Lepidinm meyenii Walp function
Beverage.
Embodiment 2
A kind of immunity regulating type Maca functional drink, material composition be in mass Lepidinm meyenii Walp juice 3kg,
Sucus Mali pumilae 10kg, acid 0.5kg, sucrose 4kg, ganoderan 0.07kg, taurine 0.06kg,
Suspension stabilizer 3kg.
Preparation method, comprises the following steps:
(1) select fresh Lepidinm meyenii Walp root, filter after squeezing the juice, take filtrate and mix with Sucus Mali pumilae,
To feed liquid；
(2) take the sucrose of 1/10 weight, after uniform to itself and suspension stabilizer dry blending, add
Entering in the water that temperature is 85 DEG C, stirring is fully dissolved, and after filtering out insoluble granule, adds
In feed liquid；
(3) residue sucrose is joined in 85 DEG C of water, after stirring is fully dissolved, join step
Suddenly in the feed liquid of (2), add ganoderan, taurine stirs；Obtain mixture
Material；
(4) acid is fully dissolved with the water of 65 DEG C, obtain acid solution；And acid solution is slowly added to
In mixed material, stir；
(5) by the material of step (4), 45 DEG C, homogenizing processes under 22MPa；
(6) use membrane aperture be 0.2 micron worry membrane filtration process, and will filter after filtrate
Load in vial, and be placed on 8 DEG C of chilling treatment, it is thus achieved that immunity regulating type Lepidinm meyenii Walp function
Beverage.Described acid is citric acid.Described sucrose, uses high fructose syrup to substitute.
Embodiment 3
A kind of immunity regulating type Maca functional drink, material composition be in mass Lepidinm meyenii Walp juice 2kg,
Sucus Mali pumilae 8kg, acid 0.4kg, sucrose 3kg, ganoderan 0.06kg, taurine 0.05kg, outstanding
Floating stabilizer 2kg.
Preparation method, comprises the following steps:
(1) select fresh Lepidinm meyenii Walp root, filter after squeezing the juice, take filtrate and mix with Sucus Mali pumilae,
To feed liquid；
(2) take the sucrose of 1/10 weight, after uniform to itself and suspension stabilizer dry blending, add
Entering in the water that temperature is 80 DEG C, stirring is fully dissolved, and after filtering out insoluble granule, adds
In feed liquid；
(3) residue sucrose is joined in 80 DEG C of water, after stirring is fully dissolved, join step
Suddenly, in the feed liquid of (2), ganoderan, taurine, Fructus Crataegi are added mixed with Radix Glycyrrhizae
Extracting solution, stirs, and obtains mixed material；
(4) acid is fully dissolved with the water of 60 DEG C, obtain acid solution；And acid solution is slowly added to
In mixed material, stir；
(5) by the material of step (4), 40 DEG C, homogenizing processes under 20MPa；
(6) use membrane aperture be 0.2 micron worry membrane filtration process, and will filter after filtrate
Load in vial, and be placed on 6 DEG C of chilling treatment, it is thus achieved that immunity regulating type Lepidinm meyenii Walp function
Beverage.Described material composition, also includes Fructus Crataegi and the mixed extracting solution of Radix Glycyrrhizae.Described
Material composition, the proportioning of its Fructus Crataegi and Radix Glycyrrhizae is mixed in equal amounts, adds the water of 10 times of Fructus Crataegi weight,
At 90 DEG C of extracting solution obtained after decocting and extracting 30min.Described material composition, extracting solution
Addition is 1kg in mass.
Embodiment 4
A kind of immunity regulating type Maca functional drink, material composition be in mass Lepidinm meyenii Walp juice 3kg,
Sucus Mali pumilae 10kg, acid 0.5kg, sucrose 4kg, ganoderan 0.07kg, taurine 0.06kg,
Suspension stabilizer 1kg.
Preparation method, comprises the following steps:
(1) select fresh Lepidinm meyenii Walp root, filter after squeezing the juice, take filtrate and mix with Sucus Mali pumilae,
To feed liquid；
(2) take the sucrose of 1/10 weight, after uniform to itself and suspension stabilizer dry blending, add
Entering in the water that temperature is 75 DEG C, stirring is fully dissolved, and after filtering out insoluble granule, adds
In feed liquid；
(3) residue sucrose is joined in 85 DEG C of water, after stirring is fully dissolved, join step
Suddenly, in the feed liquid of (2), ganoderan, taurine, Fructus Crataegi are added mixed with Radix Glycyrrhizae
Extracting solution, stirs, and obtains mixed material；
(4) acid is fully dissolved with the water of 55 DEG C, obtain acid solution；And acid solution is slowly added to
In mixed material, stir；
(5) after the material of step (4) being added appropriate amount of essence, 45 DEG C, under 18MPa all
Matter processes；
(6) use membrane aperture be 0.2 micron worry membrane filtration process, and will filter after filtrate
Load in vial, and be placed on 5 DEG C of chilling treatment, it is thus achieved that immunity regulating type Lepidinm meyenii Walp function
Beverage.Described material composition, also includes appropriate essence.Described material composition, also wraps
Include Fructus Crataegi and the mixed extracting solution of Radix Glycyrrhizae.Joining of described material composition, its Fructus Crataegi and Radix Glycyrrhizae
Ratio is mixed in equal amounts, adds the water of the Fructus Crataegi of 10 times of weight, decocts at 130 DEG C and extracts 50min
After the extracting solution that obtains.Described material composition, the addition of extracting solution is 3kg in mass.
Embodiment 5
On the basis of embodiment 3, other all with embodiment 3, described material composition, also wrap
Include Fructus Crataegi and the mixed extracting solution of Radix Glycyrrhizae.Joining of described material composition, its Fructus Crataegi and Radix Glycyrrhizae
Ratio is mixed in equal amounts, adds the water of the Fructus Crataegi of 10 times of weight, decocts at 100 DEG C and extracts 40min
After the extracting solution that obtains.Described material composition, the addition of extracting solution is calculated as with mass parts
2kg。
In order to prove the technique effect of the invention, the invention also provides for following enforcement
Example:
Experimental example 1, the test medicine impact on immunologic hypofunction mice serum hemolysin
1, laboratory animal: Kunming mouse, male and female half and half, body weight 18～22g, SPF level,
Thered is provided by Zhongshan University's Experimental Animal Center, the animal quality certification number: Guangdong probatio inspectionem pecuoarem word
SCXK (Guangdong)-2014-0011.
2, medicine packet and process:
Normal group: distilled water.
Model group: cyclophosphamide lumbar injection modeling.
Test medicine low dose group: Example 1 Maca beverage before experiment, the agent of 500ml ratio
Amount adds distilled water, and to be made into 250ml stand-by, each gavage 0.25ml of low dose group.
Dosage group in test medicine: Example 2 Maca beverage before experiment, the agent of 500ml ratio
Amount adds distilled water, and to be made into 250ml stand-by, each gavage 0.5ml of low dose group.
Test medicine high dose group: Example 3 Maca beverage before experiment, the agent of 500ml ratio
Amount adds distilled water, and to be made into 250ml stand-by, each gavage 1.0ml of low dose group.
Positive control medicine (paecilomyces hepiall chen) group: paecilomyces hepiall chen is by Jiangxi JINSHUIBAO pharmacy
Company limited produces, lot number: 140907.Paecilomyces hepiall chen content is taken, with distillation before experiment
Water is made into the solution that concentration is 39mg/ml.
3, reagent and instrument:
Cyclophosphamide: Hualian Pharmaceutical Co., Ltd., Shanghai produces, lot number: 20140714.Experiment
Front physiology salt
Water is made into the solution that concentration is 5mg/ml.
Glucose: analytical pure, Guangzhou Chemical Reagent Factory produces, lot number: 20110601-3.
Sodium citrate: analytical pure, Guangzhou Chemical Reagent Factory produces, lot number: 20130904-1.
Citric acid: analytical pure, Guangzhou Chemical Reagent Factory produces, lot number: 20140404-1.
Sodium chloride: analytical pure, Shantou City's brilliance laboratory, lot number: 20131208.
Chicken erythrocyte suspension: venous blood collection under the chicken wing under aseptic condition, puts into and is equivalent to Sanguis Gallus domesticus body
Alsever ' the s liquid of long-pending 5 times, 4 DEG C of preservations.Face the used time and use normal saline cyclic washing, centrifugal 3
Secondary, it is made into 5% chicken red blood cell suspension with constant hematocrit number normal saline
(Alsever ' preparation of s liquid: glucose 2.05g, sodium citrate 0.80g, citric acid 0.05g,
Sodium chloride 0.42g, double distilled water adds to 1.0L, autoclaving, 4 DEG C of preservations).
Guinea pig serum: take guinea pig whole blood by the method for Culling heart blood, is then centrifuged for obtaining serum.
Dual-beam ultraviolet-uisible spectrophotometer: Beijing Puxi General Instrument Co., Ltd,
Model: TU-1901.
Gastric perfusion needle: occupational health inspection center, Guangdong Province Experimental Animal Center customizes.
1ml syringe, small test tube, test tube rack.
4, experimental situation
Illumination: common；Ventilate: common；Cleanliness factor: regular grade；Temperature: 20 DEG C ± 1 DEG C；
Humidity: 50% ± 5%.
Laboratory animal environmental facility: occupational health inspection center, Guangdong Province Laboratory Animal Facility, closes
Lattice card number: Guangdong probatio inspectionem pecuoarem word 2011C031.
5, experimental technique
(1) take Kunming mouse 72, body weight 18～22g, be randomly divided into Normal group,
Dosage group in modeling group, test medicine low dose group, test medicine, test medicine high dose group,
Positive control medicine (paecilomyces hepiall chen) group totally 6 groups, often group 12.
(2) before experiment, water 10h, the most each group (in addition to dosage group) are can't help in mice fasting
Mice presses 0.1ml/10g body weight gastric infusion, 1 time/d, is total to 7d, wherein Normal group and ring
Phosphamide modeling group is to equal-volume distilled water.
(3) in addition to Normal group, all note in the 3d being administered to 5d abdominal cavity for remaining 10 groups
Penetrate cyclophosphamide 50mg/kg, cause immunologic hypofunction model.
(4) at test 1d every Mus lumbar injection 5% chicken erythrocyte suspension 0.2ml, stimulating animal
Immunity.
(5) 1h after last is administered, wins animal eyeball and takes blood, and 3000r/min is centrifuged 10min,
Separate serum, with normal saline by serum-dilution 100 times.
(6) dilute serum 1ml is taken, with 5% chicken erythrocyte suspension 0.5ml, 10% guinea pig serum
(complement) 0.5ml mixes, and at 37 DEG C of water-bath incubation 30min, then sets to 0 in DEG C refrigerator and stops
Reaction, is centrifuged 10min by aforesaid liquid 3000r/min, takes supernatant, can by dual-beam ultraviolet
See that spectrophotometer measures OD value at 540nm, separately set the blank being not added with mice serum
Pipe returns to zero.Serum hemolysin is represented with the OD value of sample.
6, experimental result
As shown in table 1:
Table 1
From table 1, test medicine high dose group serum hemolysin and cyclophosphamide modeling
Group compares, and has significant difference (P ＜ 0.05) statistically.Positive control medicine (gold
Water health-care capsule) group serum hemolysin compare with cyclophosphamide modeling group, there is highly significant
Sex differernce (P ＜ 0.01) but not statistically significant.
The impact on the carbon clearance of immunologic hypofunction mice of experimental example 2, test medicine
1, laboratory animal: Kunming mouse, male and female half and half, body weight 18～22g, SPF level,
Thered is provided by Zhongshan University's Experimental Animal Center, the animal quality certification number: Guangdong probatio inspectionem pecuoarem word
SCXK (Guangdong)-2014-0011.
2, medicine packet and process:
Normal group: distilled water.
Model group: with cyclophosphamide lumbar injection modeling.
Test medicine low dose group: take before experiment and be equivalent to Maca beverage formula 1,500ml ratio
Dosage add distilled water to be made into 250ml stand-by, each gavage 0.25ml of low dose group.
Dosage group in test medicine: take before experiment and be equivalent to Maca beverage formula 1,500ml ratio
Dosage add distilled water to be made into 250ml stand-by, each gavage 0.5ml of low dose group.
Test medicine high dose group: take before experiment and be equivalent to Maca beverage formula 1,500ml ratio
Dosage add distilled water to be made into 250ml stand-by, each gavage 1.0ml of low dose group.
Positive control medicine (paecilomyces hepiall chen) group: paecilomyces hepiall chen is by Jiangxi JINSHUIBAO pharmacy
Company limited produces, lot number: 140907.Paecilomyces hepiall chen content is taken, with distillation before experiment
Water is made into the solution that concentration is 39mg/ml.
3, reagent and instrument:
Cyclophosphamide: Hualian Pharmaceutical Co., Ltd., Shanghai produces, lot number: 20140714.Experiment
Front physiology salt
Water is made into the solution that concentration is 5mg/ml.
Sodium carbonate: analytical pure, Guangzhou Chemical Reagent Factory produces, lot number: 20110403-2.Real
Test front distillation
Water is made into the sodium carbonate liquor that concentration is 0.1%.
Dual-beam ultraviolet-uisible spectrophotometer: Beijing Puxi General Instrument Co., Ltd,
Model: TU-1901.
Gastric perfusion needle: occupational health inspection center, Guangdong Province Experimental Animal Center customizes.
1ml syringe, small test tube, test tube rack.
4, experimental situation
Illumination: common；Ventilate: common；Cleanliness factor: regular grade；Temperature: 20 DEG C ± 1 DEG C；
Humidity: 50% ± 5%.
Laboratory animal environmental facility: occupational health inspection center, Guangdong Province Laboratory Animal Facility, closes
Lattice card number: Guangdong probatio inspectionem pecuoarem word 2011C031.
5, experimental technique
Take Kunming mouse 72, body weight 18～22g, be randomly divided into Normal group, ring phosphinylidyne
Dosage group, test medicine high dose in amine modeling group, test medicine low dose group, test medicine
Group, positive control medicine (paecilomyces hepiall chen) group totally 6 groups, often group 12, mice before experiment
Water 10h is can't help in fasting, respectively organizes mice (in addition to dosage group) by 0.1ml/10g body weight
Gastric infusion, 1 time/d, altogether 7d, wherein Normal group and cyclophosphamide modeling group such as give at the body
Long-pending distilled water.In addition to Normal group, all note in the 3d being administered to 5d abdominal cavity for remaining 10 groups
Penetrate cyclophosphamide 50mg/kg, cause immunologic hypofunction model.1h after last administration is each little
Tail vein injection india ink 0.1ml/10g body weight, 2min (T after injection1)、12min
(T2) take blood 20 μ l with the suction pipe of heparin sodium moistening from orbital vein respectively, it is dissolved in 2ml's
In 0.1% sodium carbonate liquor, shake up, with dual-beam ultraviolet-uisible spectrophotometer in 680nm ripple
Strong point surveys OD value, finally by mice sacrificed by exsanguination, takes liver spleen and claims its quality, calculate and clean up finger
Index is cleaned up in number, correction.
Clean up index K=(logA1-logA2)/(T2-T1), A in formula1、A2Respectively 2min,
The OD value of 12min.
6, experimental result
As shown in table 2:
Table 2
Note: compare with cyclophosphamide modeling group:*P ＜ 0.05；**P ＜ 0.01.
By table 2 finding, the correction of test medicine middle and high dosage group is cleaned up index and is made with cyclophosphamide
Module compares, and has significant differences (P ＜ 0.01) statistically.Positive control drug
Thing paecilomyces hepiall chen group compares with cyclophosphamide modeling group, has very significant statistically
Difference (P ＜ 0.01).
To sum up, the immunity regulating type Maca functional drink of the present invention, make Lepidinm meyenii Walp, ganoderan and
Taurine nutrition and health care are effectively combined, and improve Maca beverage health-care effect, drink
The clear mesh in back is felt well, and often drinking can enhancing immunity.
It is important to point out at this, above-described embodiment and experimental example are only limitted to the skill to the present invention
Art scheme is further understood from and illustrates, does not do technical scheme further
Limit, the essential characteristics of the non-protruding that those skilled in the art make on this basis and non-aobvious
Write progressive improvement, belong to the protection category of the present invention.
Claims (10)
1. an immunity regulating type Maca functional drink, it is characterised in that material composition is with matter
Amount part is calculated as Lepidinm meyenii Walp juice 1-3 part, Sucus Mali pumilae 5-10 part, acid 0.3-0.5 part, sucrose 2-4 part, spirit
Sesame polysaccharide 0.05-0.07 part, taurine 0.04-0.06 part, suspension stabilizer 1-3 part.
2. immunity regulating type Maca functional drink as claimed in claim 1, it is characterised in that
Described material composition is calculated as 2 parts of Lepidinm meyenii Walp juice, Sucus Mali pumilae 8 parts, acid 0.4 part, sugarcane with mass parts
Sugar 3 parts, ganoderan 0.06 part, taurine 0.05 part, suspension stabilizer 2 parts.
3. immunity regulating type Maca functional drink as claimed in claim 1, it is characterised in that
Described material composition is calculated as 3 parts of Lepidinm meyenii Walp juice, Sucus Mali pumilae 10 parts, acid 0.5 part, sugarcane with mass parts
Sugar 4 parts, ganoderan 0.07 part, taurine 0.06 part, suspension stabilizer 1 part.
4. the immunity regulating type Maca functional drink as described in claim 1 or 2 or 3, its feature
It is that described material composition also includes appropriate essence.
5. the immunity regulating type Maca functional drink as described in claim 1 or 2 or 3, its feature
It is, described material composition, also includes Fructus Crataegi and the mixed extracting solution of Radix Glycyrrhizae.
6. immunity regulating type Maca functional drink as claimed in claim 5, it is characterised in that
Described material composition, the proportioning of its Fructus Crataegi and Radix Glycyrrhizae is mixed in equal amounts, adds 10 times of weight
The water of Fructus Crataegi, at the 90-130 DEG C of extracting solution obtained after decocting and extracting 30-50min.
7. immunity regulating type Maca functional drink as claimed in claim 5, it is characterised in that
Described material composition, the addition of extracting solution is calculated as 1-3 part with mass parts.
8. immunity regulating type Maca functional drink as claimed in claim 1, it is characterised in that
Described acid is at least one in citric acid, malic acid.
9. immunity regulating type Maca functional drink as claimed in claim 1, it is characterised in that
Described sucrose, uses at least one in high fructose syrup, sweet sugar TR100 to substitute.
10. the system of the immunity regulating type Maca functional drink as described in any one of claim 1-9
Preparation Method, it is characterised in that comprise the following steps:
(1) select fresh Lepidinm meyenii Walp root, filter after squeezing the juice, take filtrate and mix with Sucus Mali pumilae,
To feed liquid；
(2) sucrose of 1/10 weight portion is taken, after uniform to itself and suspension stabilizer dry blending,
Joining in the water that temperature is 75-85 DEG C, stirring is fully dissolved, after filtering out insoluble granule,
Join in feed liquid；
(3) residue sucrose is joined in 75-85 DEG C of water, after stirring is fully dissolved, join
In the feed liquid of step (2), add ganoderan, taurine stirs；Or add
Ganoderan, taurine, Fructus Crataegi and the mixed extracting solution of Radix Glycyrrhizae, stir, mixed
Compound material；
(4) water of acid 55-65 DEG C is fully dissolved, obtain acid solution；And acid solution is slowly added
Enter in mixed material, stir；
(5) after the material of step (4) being added essence or being added without essence, 35-45 DEG C,
Under 18-22MPa, homogenizing processes；
(6) use membrane aperture be 0.2 micron worry membrane filtration process, and will filter after filtrate
Load in vial, and be placed on 2-8 DEG C of chilling treatment, it is thus achieved that immunity regulating type Lepidinm meyenii Walp merit
Can beverage.
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