CN105936869A - Multifunctional fluorescent quantitative PCR instrument - Google Patents

Multifunctional fluorescent quantitative PCR instrument Download PDF

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Publication number
CN105936869A
CN105936869A CN201610459991.9A CN201610459991A CN105936869A CN 105936869 A CN105936869 A CN 105936869A CN 201610459991 A CN201610459991 A CN 201610459991A CN 105936869 A CN105936869 A CN 105936869A
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CN
China
Prior art keywords
gear
motor
pcr instrument
electric pushrod
main frame
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610459991.9A
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Chinese (zh)
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CN105936869B (en
Inventor
任峻
张华忠
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ZHEJIANG AIYI BIOMEDICAL TECHNOLOGY Co Ltd
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ZHEJIANG AIYI BIOMEDICAL TECHNOLOGY Co Ltd
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Priority to CN201610459991.9A priority Critical patent/CN105936869B/en
Publication of CN105936869A publication Critical patent/CN105936869A/en
Application granted granted Critical
Publication of CN105936869B publication Critical patent/CN105936869B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14Process control and prevention of errors

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a multifunctional fluorescent quantitative PCR instrument, and belongs to the technical field of gene detection equipment. The instrument comprises a first electric push rod, a multistage telescoping rod, a first moving block, a first linear motor, a second linear motor, a first clamping plate, a lid, a second gear, a second motor, a belt, a wheel carrier, a first motor, rollers, a support plate, a third sliding block, a third slide rail, a rotation shaft, a cylinder, a first flange bearing, a second slide rail, a platen, a second sliding block, a second flange bearing, a second electric push rod, a first gear, a third gear, a bearing groove, a carrier, a first embossment, a controller, a sample tube, a PCR instrument host, a turnover cover, a second clamping plate, a second moving block, and a casing. The turnover cover is arranged on the PCR instrument host. The wheel carrier is arranged in the PCR instrument host. The left side and right side of the wheel carrier are both provided with a roller. The provided instrument can automatically cover a sample tube.

Description

A kind of Multifunction fluorescent quantitative PCR apparatus
Technical field
The present invention relates to a kind of Multifunction fluorescent quantitative PCR apparatus, belong to gene detection equipment technical field.
Background technology
Quantitative real time PCR Instrument is specific target gene detection and quantitative unified platform, and by PCR thermal cycle, fluorescence is examined Survey and various analysis softwares combine, can dynamically observe in the PCR each reaction tube of each circulation pcr amplification product by Cumulative situation about adding.The most automatically cover the function of sample cell inside the PCR instrument of prior art, need people manually to be covered by plastic foil Cover on sample cell, use very inconvenient.In order to solve above-mentioned difficulties, need to develop and a can automatically cover sample cell Multifunction fluorescent quantitative PCR apparatus.
Summary of the invention
It is an object of the invention to provide a kind of Multifunction fluorescent quantitative PCR apparatus.
The problem to be solved in the present invention is the most automatically to cover the asking of function of sample cell inside the PCR instrument of prior art Topic.
For realizing the purpose of the present invention, the technical solution used in the present invention is:
A kind of Multifunction fluorescent quantitative PCR apparatus, including the first electric pushrod, multistage retractable rod, the first movable block, the first straight-line electric Machine, the second linear electric motors, the first clamping plate, lid, the second gear, the second motor, belt, wheel carrier, the first motor, roller, support plate, 3rd slide block, the 3rd slide rail, rotating shaft, cylinder, the first flange bearing, the second slide rail, platen, the second slide block, the second flange bearing, Second electric pushrod, the first gear, the 3rd gear, backing groove, bracket, the first projection, controller, sample cell, PCR instrument master Machine, renovate, the second clamping plate, the second movable block and casing, described in renovate and be arranged on PCR instrument main frame, wheel is installed in PCR instrument main frame Frame, wheel carrier is each side installed roller, and belt is arranged on two rollers, and the axle of the roller being positioned at lower section passes through shaft coupling Being connected with the first motor, the first motor is arranged on roller, and in installing casing, casing in PCR instrument main frame, right side installation the 3rd is sliding Rail, the 3rd slide rail is installed the 3rd slide block, and the 3rd slide block is installed support plate, and casing left side wall is provided with breach, and support plate is stretched from breach Going out outside casing, be arranged on belt on the left of support plate, in support plate is placed lid, PCR instrument main frame, the first linear electric motors are installed in left side, First clamping plate are installed on the left of the first linear electric motors, the first linear electric motors are installed the first movable block, the first movable block is installed many Level expansion link, multistage retractable rod front end installs the first electric pushrod, installs the second linear electric motors under the first electric pushrod, and second is straight Second movable block is installed on line motor, the second clamping plate are installed under the second movable block, cylinder is installed in PCR instrument main frame, cylinder is pacified Dress platen, installs the second slide block on the right side of platen, the second slide block is arranged on the second slide rail, and platen is installed three the second flange shaft Hold, the second flange bearing is installed the second electric pushrod, groove at the bottom of the second electric pushrod front end mounting bracket, the second electric pushrod side Installing the first gear on wall, install the second gear between two first gears in left side, the second gear is arranged on the second motor On output shaft, the second motor is arranged on platen, installs the 3rd gear between the first gear and first gear of centre on right side, 3rd gear is arranged in rotating shaft, and rotating shaft is arranged on the first flange bearing, and the first flange bearing is arranged on platen, PCR instrument Right side mounting bracket in main frame, bracket is provided with duck eye, and sample cell is placed on duck eye, sample cell lateral wall is installed first convex Rising, controller is arranged on PCR instrument main frame, controller by wire respectively with the first motor, the second motor, first electronic push away Bar, the second electric pushrod, the first linear electric motors, the second linear electric motors are connected with multistage retractable rod.
Described cylinder internal solenoid valve, electromagnetic valve is connected with controller by wire.
Described lid includes that overlay, annular sleeve and second are protruding, and overlay is arranged in annular sleeve, annular It is protruding that set medial wall installs second.
The invention have the advantage that controller controls the first electric motor starting, make roller rotate, make belt move up, make to prop up Plate moves up, and makes lid move up;Controller controls the first linear electric motors and starts, and makes the first movable block move up and down;Control Device processed controls multistage retractable rod and does stretching motion, makes the first clamping plate move right, is moved to above sample cell by lid;Controller Control the first electric pushrod and do stretching motion, make the second linear electric motors move up and down;Controller controls the second linear electric motors and starts, Making the second movable block move left and right, make the second clamping plate move left and right, make the second clamping plate and the first clamping plate clamp lid, lid moves Above sample cell, controller controls the first electric pushrod and does stretching, makes lid move down, and makes cap covers sample Pipe, the first electric pushrod promotes lid to make the second projection move to, under the first projection, be fixed by lid;Controller controls cylinder Do stretching motion, make platen move up;Controller controls the second electric pushrod and does stretching motion, makes backing groove upwards move Dynamic, make backing groove entangle sample cell lower end;Controller controls the second electric motor starting, makes the second gear rotate, makes the first gear Rotate with the 3rd gear, make backing groove rotate with sample cell;The sample in sample cell is made to obtain uniform illumination.
Accompanying drawing explanation
Fig. 1 is one Multifunction fluorescent quantitative PCR apparatus overall structure figure of the present invention;
Fig. 2 is the structure enlarged diagram of lid;
In figure: 1, the first electric pushrod 2, multistage retractable rod the 3, first movable block the 4, first linear electric motors the 5, second straight-line electric Machine the 6, first clamping plate 7, lid the 8, second gear the 9, second motor 10, belt 11, wheel carrier the 12, first motor 13, rolling Wheel 14, support plate the 15, the 3rd slide block the 16, the 3rd slide rail 17, rotating shaft 18, cylinder the 19, first flange bearing 20, second are slided Rail 21, platen the 22, second slide block the 23, second flange bearing the 24, second electric pushrod the 25, first gear the 26, the 3rd tooth Wheel 27, backing groove 28, bracket the 29, first projection 30, controller 31, sample cell 32, PCR instrument main frame 33, renovate 34, second clamping plate the 35, second movable block 36, overlay 37, annular sleeve the 38, second projection 39, casing.
Detailed description of the invention
Below in conjunction with the accompanying drawings and embodiment the present invention is further illustrated.
One Multifunction fluorescent quantitative PCR apparatus of the present invention, including the first electric pushrod 1, multistage retractable rod 2, first moves Block the 3, first linear electric motors the 4, second linear electric motors the 5, first clamping plate 6, lid the 7, second gear the 8, second motor 9, belt 10, wheel Frame the 11, first motor 12, roller 13, support plate the 14, the 3rd slide block the 15, the 3rd slide rail 16, rotating shaft 17, cylinder the 18, first flange shaft Hold the 19, second slide rail 20, platen the 21, second slide block the 22, second flange bearing the 23, second electric pushrod the 24, first gear 25, Three gears 26, backing groove 27, bracket the 28, first projection 29, controller 30, sample cell 31, PCR instrument main frame 32, renovate 33, Second splint the 34, second movable block 35 and casing 39, described in renovate 33 and be arranged on PCR instrument main frame 32, install in PCR instrument main frame 32 Wheel carrier 11, wheel carrier 11 is each side installed roller 13, and belt 10 is arranged on two rollers 13, is positioned at the roller 13 of lower section Axle be connected by shaft coupling and the first motor 12, the first motor 12 is arranged on roller 13, and controller 30 controls the first motor 12 start, and make roller 13 rotate, make belt 10 move up, make support plate 14 move up, make lid 7 move up;PCR instrument master Installing casing 39 in machine 32, in casing 39, the 3rd slide rail 16 is installed on right side, and the 3rd slide rail 16 is installed the 3rd slide block 15, and the 3rd is sliding Installing support plate 14 on block 15, casing 39 left side wall is provided with breach, and support plate 14, from breach stretches out casing 39, is installed on the left of support plate 14 On belt 10, support plate 14 being placed lid 7, lid 7 includes overlay 36, annular sleeve 37 and the second projection 38, transparent Plastic foil 36 is arranged in annular sleeve 37, and the second projection 38 is installed in annular sleeve 37 medial wall, and in PCR instrument main frame 32, left side installs the One linear electric motors 4, controller 30 controls the first linear electric motors 4 and starts, and makes the first movable block 3 move up and down;First linear electric motors 4 The first clamping plate 6 are installed in left side, and the first linear electric motors 4 are installed the first movable block 3, and the first movable block 3 is installed multistage retractable rod 2, controller 30 controls multistage retractable rod 2 and does stretching motion, makes the first clamping plate 6 move right, lid 7 is moved to sample cell 31 Top;The first electric pushrod 1 is installed in multistage retractable rod 2 front end, and controller 30 controls the first electric pushrod 1 and does stretching motion, makes Second linear electric motors 5 move up and down;First electric pushrod 1 time installs the second linear electric motors 5, and the second linear electric motors 5 are installed the Two movable blocks 35, second movable block 35 times installation the second clamping plate 34, controller 30 controls the second linear electric motors 5 and starts, makes second Movable block 35 moves left and right, and makes the second clamping plate 34 move left and right, and makes the second clamping plate 34 and the first clamping plate 6 clamp lid 7, lid 7 Moving to above sample cell 31, controller 30 controls the first electric pushrod 1 and does stretching, makes lid 7 move down, makes lid 7 cover sample cell 31, and the first electric pushrod 1 promotes lid 7 to make the second projection 38 move to first protruding 29 times, by solid for lid 7 Determine to live;Installing cylinder 18, cylinder 18 internal solenoid valve in PCR instrument main frame 32, electromagnetic valve is connected with controller 30 by wire, control Device 30 processed controls cylinder 18 and does stretching motion, makes platen 21 move up;Loading stand 21 on cylinder 18, installs on the right side of platen 21 Second slide block 22, the second slide block 22 is arranged on the second slide rail 20, and platen 21 is installed three the second flange bearings 23, the second method Installing the second electric pushrod 24, groove 27 at the bottom of the second electric pushrod 24 front end mounting bracket on blue bearing 23, controller 30 controls the Two electric pushrods 24 do stretching motion, make backing groove 27 move up, and make backing groove 27 entangle sample cell 31 lower end;Second First gear 25 is installed on electric pushrod 24 sidewall, between two first gears 25 in left side, the second gear 8, the second gear is installed On 8 output shafts being arranged on the second motor 9, the second motor 9 is arranged on platen 21, first gear 25 on right side and middle the Installing the 3rd gear 26 between one gear 25, the 3rd gear 26 is arranged in rotating shaft 17, and rotating shaft 17 is arranged on the first flange bearing On 19, the first flange bearing 19 is arranged on platen 21, and right side mounting bracket 28 in PCR instrument main frame 32, bracket 28 is provided with little Hole, sample cell 31 is placed on duck eye, and sample cell 31 lateral wall is installed the first projection 29, and controller 30 controls the second motor 9 Start, make the second gear 8 rotate, make the first gear 25 and the 3rd gear 26 rotate, make backing groove 27 revolve with sample cell 31 Turn, make the sample in sample cell 31 obtain uniform illumination;Controller 30 is arranged on PCR instrument main frame 32, and controller 30 passes through Wire respectively with first motor the 12, second motor the 9, first electric pushrod the 1, second electric pushrod the 24, first linear electric motors 4, Two linear electric motors 5 are connected with multistage retractable rod 2.
Using method of the present invention: controller 30 controls the first motor 12 and starts, and makes roller 13 rotate, and makes belt 10 upwards move Dynamic, make support plate 14 move up, make lid 7 move up;Controller 30 controls the first linear electric motors 4 and starts, and makes first to move Block 3 moves up and down;Controller 30 controls multistage retractable rod 2 and does stretching motion, makes the first clamping plate 6 move right, is moved by lid 7 Above sample cell 31;Controller 30 controls the first electric pushrod 1 and does stretching motion, makes the second linear electric motors 5 move up and down;Control Device 30 processed controls the second linear electric motors 5 and starts, and makes the second movable block 35 move left and right, and makes the second clamping plate 34 move left and right, and makes Lid 7 clamped by second splint 34 and the first clamping plate 6, and lid 7 moves to above sample cell 31, and controller 30 controls the first electric pushrod 1 does stretching, makes lid 7 move down, and makes lid 7 cover sample cell 31, and the first electric pushrod 1 promotes lid 7 to make second Protruding 38 move to first protruding 29 times, are fixed by lid 7;Controller 30 controls cylinder 18 and does stretching motion, makes platen 21 Move up;Controller 30 controls the second electric pushrod 24 and does stretching motion, makes backing groove 27 move up, makes backing groove 27 entangle sample cell 31 lower end;Controller 30 controls the second motor 9 and starts, and makes the second gear 8 rotate, and makes the first gear 25 and Three gears 26 rotate, and make backing groove 27 rotate with sample cell 31;The sample in sample cell 31 is made to obtain uniform illumination.

Claims (3)

1. a Multifunction fluorescent quantitative PCR apparatus, including the first electric pushrod (1), multistage retractable rod (2), the first movable block (3), the first linear electric motors (4), the second linear electric motors (5), the first clamping plate (6), lid (7), the second gear (8), the second motor (9), belt (10), wheel carrier (11), the first motor (12), roller (13), support plate (14), the 3rd slide block (15), the 3rd slide rail (16), rotating shaft (17), cylinder (18), the first flange bearing (19), the second slide rail (20), platen (21), the second slide block (22), Two flange bearings (23), the second electric pushrod (24), the first gear (25), the 3rd gear (26), backing groove (27), bracket (28), the first protruding (29), controller (30), sample cell (31), PCR instrument main frame (32), renovate (33), the second clamping plate (33), Second movable block (34) and casing (39), is characterized in that: described in renovate (33) and be arranged on PCR instrument main frame (32), PCR instrument main frame (32) installing wheel carrier (11) in, wheel carrier (11) is each side installed roller (13), belt (10) is arranged on two rollers (13), on, the axle of the roller (13) being positioned at lower section is connected by shaft coupling and the first motor (12), and the first motor (12) is arranged on On roller (13), in installing casing (39), casing (39) in PCR instrument main frame (32), the 3rd slide rail (16) is installed on right side, and the 3rd is sliding Rail (16) is upper installs the 3rd slide block (15), and the 3rd slide block (15) is installed support plate (14), and casing (39) left side wall is provided with breach, Plate (14) is from breach stretches out casing (39), and support plate (14) left side is arranged on belt (10), and support plate places lid on (14) (7), in PCR instrument main frame (32), the first linear electric motors (4) are installed in left side, and the first clamping plate are installed in the first linear electric motors (4) left side (6), the first linear electric motors (4) are upper installs the first movable block (3), and the first movable block (3) is installed multistage retractable rod (2), multistage The first electric pushrod (1) is installed in expansion link (2) front end, installs the second linear electric motors (5) under the first electric pushrod (1), and second is straight Line motor (5) is upper installs the second movable block (34), installs the second clamping plate (33), PCR instrument main frame (32) under the second movable block (34) Interior installation cylinder (18), the upper loading stand (21) of cylinder (18), the second slide block (22), the second slide block are installed in platen (21) right side (22) being arranged on the second slide rail (20), platen (21) is upper installs three the second flange bearings (23), the second flange bearing (23) Upper installation the second electric pushrod (24), groove (27) at the bottom of the mounting bracket of the second electric pushrod (24) front end, the second electric pushrod (24) First gear (25) is installed on sidewall, between two first gears (25) in left side, the second gear (8), the second gear (8) is installed Being arranged on the output shaft of the second motor (9), the second motor (9) is arranged on platen (21), first gear (25) on right side and Installing the 3rd gear (26) between middle the first gear (25), the 3rd gear (26) is arranged in rotating shaft (17), rotating shaft (17) Being arranged on the first flange bearing (19), the first flange bearing (19) is arranged on platen (21), right side in PCR instrument main frame (32) Mounting bracket (28), bracket (28) is provided with duck eye, and sample cell (31) is placed on duck eye, and sample cell (31) lateral wall is installed First protruding (29), controller (30) is arranged on PCR instrument main frame (32), controller (30) by wire respectively with the first motor (12), the second motor (9), the first electric pushrod (1), the second electric pushrod (24), the first linear electric motors (4), the second straight-line electric Machine (5) is connected with multistage retractable rod (2).
A kind of Multifunction fluorescent quantitative PCR apparatus the most according to claim 1, is characterized in that: described cylinder (18) in-built electrical Magnet valve, electromagnetic valve is connected with controller (30) by wire.
A kind of Multifunction fluorescent quantitative PCR apparatus the most according to claim 1, is characterized in that: described lid (7) includes transparent Plastic foil (36), annular sleeve (37) and second are protruding (38), and overlay (36) is arranged in annular sleeve (37), annular sleeve (37) second protruding (38) are installed in medial wall.
CN201610459991.9A 2016-06-23 2016-06-23 A kind of Multifunction fluorescent quantitative PCR apparatus Expired - Fee Related CN105936869B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610459991.9A CN105936869B (en) 2016-06-23 2016-06-23 A kind of Multifunction fluorescent quantitative PCR apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610459991.9A CN105936869B (en) 2016-06-23 2016-06-23 A kind of Multifunction fluorescent quantitative PCR apparatus

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CN105936869A true CN105936869A (en) 2016-09-14
CN105936869B CN105936869B (en) 2018-05-25

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106244446A (en) * 2016-10-14 2016-12-21 上海爱易生物医学科技股份有限公司 A kind of scalable quantitative real time PCR Instrument
CN106318863A (en) * 2016-10-14 2017-01-11 上海爱易生物医学科技股份有限公司 System for gene detection
CN106479860A (en) * 2016-10-14 2017-03-08 上海爱易生物医学科技股份有限公司 A kind of self-sealing quantitative real time PCR Instrument
CN106497775A (en) * 2016-10-14 2017-03-15 上海爱易生物医学科技股份有限公司 A kind of quantitative real time PCR Instrument with automatic sealing function

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101363001A (en) * 2008-08-22 2009-02-11 金银杏生物科技(北京)有限公司 Sliding mode heat transfer media plate PCR instrument
CN203976778U (en) * 2014-06-16 2014-12-03 杭州安杰思生物科技有限公司 The hot lid mechanism of a kind of automatic shutter for gene-amplificative instrament
CN204509343U (en) * 2015-03-03 2015-07-29 杭州博日科技有限公司 PCR quantitative fluorescence analysis instrument heat lid
CN104962453A (en) * 2015-06-04 2015-10-07 南京普朗医疗设备有限公司 Device for amplification and reaction of tube cap gene chip
CN105400680A (en) * 2015-12-11 2016-03-16 舟山医院 A novel fluorescent quantitative PCR instrument

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101363001A (en) * 2008-08-22 2009-02-11 金银杏生物科技(北京)有限公司 Sliding mode heat transfer media plate PCR instrument
CN203976778U (en) * 2014-06-16 2014-12-03 杭州安杰思生物科技有限公司 The hot lid mechanism of a kind of automatic shutter for gene-amplificative instrament
CN204509343U (en) * 2015-03-03 2015-07-29 杭州博日科技有限公司 PCR quantitative fluorescence analysis instrument heat lid
CN104962453A (en) * 2015-06-04 2015-10-07 南京普朗医疗设备有限公司 Device for amplification and reaction of tube cap gene chip
CN105400680A (en) * 2015-12-11 2016-03-16 舟山医院 A novel fluorescent quantitative PCR instrument

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106244446A (en) * 2016-10-14 2016-12-21 上海爱易生物医学科技股份有限公司 A kind of scalable quantitative real time PCR Instrument
CN106318863A (en) * 2016-10-14 2017-01-11 上海爱易生物医学科技股份有限公司 System for gene detection
CN106479860A (en) * 2016-10-14 2017-03-08 上海爱易生物医学科技股份有限公司 A kind of self-sealing quantitative real time PCR Instrument
CN106497775A (en) * 2016-10-14 2017-03-15 上海爱易生物医学科技股份有限公司 A kind of quantitative real time PCR Instrument with automatic sealing function
CN106244446B (en) * 2016-10-14 2018-10-09 上海爱易生物医学科技股份有限公司 A kind of adjustable fluorescence quantitative PCR instrument
CN106497775B (en) * 2016-10-14 2019-03-15 上海爱易生物医学科技股份有限公司 A kind of fluorescence quantitative PCR instrument with automatic sealing function
CN106479860B (en) * 2016-10-14 2019-10-15 上海爱易生物医学科技股份有限公司 A kind of self-sealing fluorescence quantitative PCR instrument

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Granted publication date: 20180525