CN101363001A - Sliding mode heat transfer media plate PCR instrument - Google Patents
Sliding mode heat transfer media plate PCR instrument Download PDFInfo
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- CN101363001A CN101363001A CNA200810118677XA CN200810118677A CN101363001A CN 101363001 A CN101363001 A CN 101363001A CN A200810118677X A CNA200810118677X A CN A200810118677XA CN 200810118677 A CN200810118677 A CN 200810118677A CN 101363001 A CN101363001 A CN 101363001A
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Abstract
The invention discloses a sliding type heat transfer media plate PCR instrument, comprising a machine shell, a plurality of heat transfer media plates which can set the temperature in advance and a sample holder for placing a sample tube, wherein, a plurality of heat transfer media plates are arranged below the sample holder, the vertical distances between the upper surfaces of the heat transfer media plates to the sample holder are equal, the heat transfer media plates and the sample holder can be correspondingly slid under the action of a transmission device, the sliding trajectory is on the same plane, and the sample tube is indirectly or directly contacted with the upper surfaces of the different heat transfer media plates which set the different temperatures in advance according to a certain procedure during the sliding process. The PCR instrument can avoid the plugging-in and pulling-out operations of the sample tube, thereby simplifying a mechanical mobile device, simplifying a control circuit and a structure, reducing the size of the instrument, reducing the mechanical faults, reducing the cost and avoiding the harmful excessive temperature decrease of a sample caused by being exposed in air.
Description
Technical field
The present invention relates to the Bioexperiment instrument, particularly a kind of polymerase chain reaction instrument---PCR instrument.
Background technology
The PCR reaction is a kind of the most frequently used molecular biotechnology, and the PCR instrument is a PCR reaction imperative equipment.The PCR instrument has the heating and cooling of program to sample, through temperature cycle many times to reach the purpose of amplification gene.Each temperature cycle of its program has 3 temperature spots usually: first is a denaturation temperature, is generally 94～98 ℃; Second is annealing temperature, is generally 50～65 ℃, sets as required; The 3rd is elongating temperature, is generally 68～74 ℃.When PCR reaction beginning, the PCR instrument is heated to denaturation temperature with sample, and keeps several seconds.Then sample temperature is dropped to annealing temperature, and keep several seconds.Then sample is heated to elongating temperature, and keeps for some time, it is fixed that this time is come by concrete reaction conditions.And then sample is heated to denaturation temperature, begin next temperature cycle.The key technical indexes of PCR instrument is the precision of temperature and the speed of heating and cooling.The user of PCR instrument generally wishes to have temperature rate faster.This is not only in order to accelerate whole PCR process, improves people's work efficient, also is the primer wrong start (mis-priming) that may bring in temperature-fall period in order to reduce.Improving PCR instrument alternating temperature speed, particularly rate of temperature fall, is the challenge of a technology.
Existing P CR instrument from a certain angle, can be divided into two major types.The PCR instrument of the first kind is that cold/heat source is fixed on heat conduction good heat transfer media one end, sample hose is placed in the other end of heat catalysis.In the process of falling/heating up, the suction/heat release of media is changed the temperature of media, and then transfer heat into the PCR sample, to change the temperature of sample by media by cold/heat source.Heat catalysis mostly is aluminum greatly, because the heat conductivility of aluminium is good, and light weight, low price.Can make the better media of performance with copper and silver, but cost an arm and a leg, seldom adopt.The heating ratio of heat catalysis is easier to realize, such as just can be used as stable performance, little, the low-cost thermal source of volume with logical the powering on of nichrome wire.But realize that fast-refrigerating is just relatively more difficult.PCR instrument with compressor cooling was once arranged in history.The main drawback of compressor is that weight is big, volume is big, noise is big.Also the mobile tap water of Ceng Youyong is made the PCR instrument of low-temperature receiver with the thermal absorption on the media.But tap water uses inconvenient, and rate of temperature fall is subjected to the temperature effect of tap water, and when water temperature was higher, the temperature difference of low-temperature receiver and media was little, and the speed of cooling is restricted.With respect to above these early stage technology, TE sheet (thermoelectriceffect sheet, semiconductor chilling plate, Peltier cooling piece) can heat up, and can lower the temperature again, and is simple in structure, and more convenient, the PCR instrument uses the TE sheet as cold/heat source mostly at present.The PCR instrument of this first kind is modal in the market, but this type of PCR instrument has following shortcoming:
1) relative PCR sample, the thermal inertia of heat catalysis is very big.This is because its quality is big, specific heat capacity is big.Under the situation of given cold/heat source power, thermal inertia is big greatly more, and temperature change is slow more.In order to reduce the thermal inertia of media, need reduce its volume.Some expensive goods are pruned part metals with the means of mechanical workout and are reduced the volume of mode heat transfer media plate.But thermal conductivity be with conduction orientation on sectional area be directly proportional, when the sectional area of media reduced, the thermal conduction meeting was slack-off.The media volume is too little also can to cause non-uniform temperature.Be subjected to these effects limit, the thermal inertia of the heat catalysis after processing improves is still very big, and the speed of heating and cooling is still slow.
2) in order to accelerate the speed of heating and cooling, the powerful cold/heat source of needs use strengthens the temperature difference between mode heat transfer media plate and the cold/heat source.But when cold/heat source power was very big, temperature was not easy accurate control, surpassed preset value when being easy to cause heating and cooling.In order to prevent to surpass preset value, temperature controlled circuit and program be more complicated all.Therefore in actual use, the speed of heating and cooling also is subjected to the restriction of the accurate control of temperature aspect.
3) the TE sheet that is used for the PCR instrument damages easily.In order to reach rapid temperature rise and drop, the temperature difference at TE sheet two ends must be very big, and this makes the different sites of TE sheet expand asynchronously and shrink, so its ceramics and semiconductor column combining site stress changes are very big, and fracture appears in junction easily.The damage of this kind TE sheet is that the modal technology of PCR instrument is damaged.In order to prolong the life-span of TE sheet, reduce STRESS VARIATION, cut into small-sized TE sheet and replace the big TE sheet of a monoblock by many, so just make PCR instrument complex structure, Installation and Debugging program complexity, cost raises.
4) high-power in order to add, not only to also need big radiator element and big fan with powerful TE sheet.This makes PCR instrument volume and weight strengthen again, and very big noise is arranged during work.
The PCR instrument of second type is to be preheating to the heat catalysis contact PCR sample of set temperature value.So just avoided the above-mentioned slow problem of temperature rate that causes by heat catalysis.Common embodiment has following two kinds.A kind of is anemopyretic, and its media is an air, and the ambient air that will be in room temperature by damper mixes with suitable proportion with the high temperature air that heated and reaches desired temperature, and then mixed air is blown on the PCR sample.The advantage of this scheme is that heat-up rate is fast, and cost is low, and is in light weight.But the air themperature that blows on the sample hose during cooling is a room temperature, and with having a narrow range of temperature of sample hose, and the specific heat capacity of air is little, and thermal conductivity is low, causes the cooling rate of sample hose slow.For uniform mixing hot and cold air, must be air agitation very fast, so the noise of fan is very big.
The media of another kind of scheme is a metal, and it is represented as RoboCycler (U.S. Stratagene company).It has several metal media plates, metal media plate is preheating to different settings earlier, with a robot arm sample is inserted specified media plate according to preset program with sample hose then, after arriving the scheduled time sample hose is extracted, insert another piece media plate again, by that analogy, reach the purpose of the temperature of orderly change PCR sample.This scheme arrives high sample with the thermal energy transfer of being stored up because each plate stably maintains on the preset temp in heating and cooling process, so be easy to improve the temperature accuracy and the alternating temperature speed of sample, saved energy consumption simultaneously.Owing to do not need to repeat cooling, reduced requirement to heat abstractor, avoided the damage of common TE sheet, even can be without the TE sheet.Identical with the PCR instrument of the above-mentioned first kind, on the metal media plate of RoboCycler the hole of matching with PCR sample hose profile is arranged.This just needs mechanical manipulator except moving in the horizontal direction, and the transmission rig of a cover movement in vertical direction will be arranged again, sample hose could be extracted from a media plate and be transferred to another media plate again and get on.And mechanical manipulator will accurately be shifted and sample accurately could be inserted in the hole corresponding on the media plate.The main drawback that is brought by mechanical manipulator is the apparatus structure complexity, volume is big, price is high.In addition, when sample is pulled out when inserting second plate, can be exposed in the air, this can make sample temperature descend 1-2 ℃, reaction causes bad influence to PCR in this cooling meeting, especially after annealing, make sample temperature be lower than set(ting)value, might cause the non-specific binding of primer and template and lead to errors initial (mis-priming).
Summary of the invention
The object of the present invention is to provide a kind of PCR instrument, avoid the shortcoming of first kind PCR instrument, keep the advantage of the second type PCR instrument, simplify mechanical mobile device simultaneously.
Technical scheme of the present invention is as follows:
A kind of PCR instrument, mode heat transfer media plate and a specimen holder that is used to place sample hose of comprising a casing, a plurality of predeterminable temperature, wherein: described a plurality of mode heat transfer media plates are arranged under the specimen holder, and their upper surface equates with the vertical range of specimen holder, under the effect of transmission mechanism, mode heat transfer media plate and specimen holder relatively sliding, sliding trace is on same plane, and sample hose contacts with the upper surface of the different heat transfer media plate that is preheating to differing temps indirectly or directly according to certain procedure in the sliding process.
Above-mentioned a plurality of mode heat transfer media plates can be arranged in a straight line, and the relative movement orbit of they and specimen holder also linearly; Also a plurality of mode heat transfer media plates can be arranged on the circular arc, the relative movement orbit of they and specimen holder is circular-arc.
A plurality of mode heat transfer media plates can be fixed in the framework, and solid filler is inserted in the space between the plate, and the upper surface of all mode heat transfer media plates and weighting material equates to the vertical range of specimen holder.
Transmission mechanism is connected with the fixing framework of mode heat transfer media plate, perhaps is connected with specimen holder.Under first kind of situation, specimen holder is a fixed with respect to casing, and mode heat transfer media plate moves with respect to casing; Second kind of situation is then just opposite, and mode heat transfer media plate is a fixed with respect to casing, and specimen holder moves with respect to casing.
Mode heat transfer media plate of the present invention adopts metal or other hot good conductors to make, and commonly used is aluminium.And the weighting material that is filled between the plate preferably adopts bilevel composite structure, the material that is the upper strata is identical with mode heat transfer media plate, and lower floor adopts thermal insulating material, for example heat-stable porous plastics or other material (such as being the matrix material of matrix with Resins, epoxy).
Each mode heat transfer media plate all has a thermal source that it is carried out preheating, and thermal source can be simple and cheap resistive heating elements, also can be thermoelectric effect element (TE sheet).
Can also increase a heating lid on specimen holder, in the PCR process, this heating lid is pressed on the PCR sample hose, the temperature of heating lid is about 100 ℃, can prevent to condense upon the sample hose top after the moisture evaporation, simultaneously, the pressure of heating lid can make sample hose and mode heat transfer media plate that excellent contact is arranged.
In specific embodiments more of the present invention, sample hose is that plane formula contacts with mode heat transfer media plate.The upper surface of mode heat transfer media plate is the plane, and specimen holder can be a simple flat board with holes, and the bottom of sample hose is the plane, and after sample hose inserted specimen holder, the bottom of sample hose directly contacted with the upper surface of mode heat transfer media plate.Also can use common PCR tapered tube to be sample hose, corresponding specimen holder then is that a upper surface has tapered hole, lower surface is a planar PCR pipe insert seat, described tapered hole is used to insert the PCR tapered tube, this PCR pipe insert seat is made with the good conductor of heat, and its lower surface contacts with the upper surface of mode heat transfer media plate.
For the thermal inertia that reduces the PCR pipe insert seat, increase the contact area of itself and mode heat transfer media plate and shorten distance between PCR pipe and the mode heat transfer media plate, in other specific embodiments of the present invention, have the strip groove of series of parallel in the bottom of PCR pipe insert seat, several rows of PCR pipe jack is separated, correspondingly process a series of strip projected parts that matches with these strip groove surfaces at the mode heat transfer media plate upper surface, these strip grooves are consistent with the relatively sliding direction of mode heat transfer media plate and PCR pipe insert seat with the trend of projection.
Technical scheme of the present invention belongs to the preheating media types, with a transmission mechanism PCR sample is contacted with the media plate that is preheating to differing temps according to certain procedure, thereby reaches the heating and cooling that program is arranged.But the places different with above-mentioned RoboCycler are, the hole that does not have the reconstitute product on mode heat transfer media plate and the plane that sample contacts, the process of laying and withdraw from mode heat transfer media plate of sample is to finish in a kind of mode of linear slide, rather than inserts as RoboCycler and extract.Like this, as long as with a mechanical mobile device simple, that precision is not high, just can realize sample laying on the differing temps mode heat transfer media plate, thereby omitted the device that moves up and down, simplify physical construction and control circuit, reduced the volume and weight of instrument, reduced mechanical fault, reduced cost, and also avoided excessively lowering the temperature by being exposed to cause in the air in the disadvantageous sample of PCR.
Description of drawings
Fig. 1 a is the structural representation of PCR instrument when specimen holder is positioned on first mode heat transfer media plate of embodiment 1;
Fig. 1 b is the structural representation of PCR instrument when specimen holder is positioned on second mode heat transfer media plate of embodiment 1.
Fig. 2 is the mode heat transfer media plate of PCR instrument of embodiment 1 and the structural representation of specimen holder.
Fig. 3 is the structural representation of the PCR instrument of embodiment 2.
Fig. 4 a is the PCR pipe insert seat of embodiment 3 and the structural representation of the mode heat transfer media plate that contacts with it;
Fig. 4 b is the PCR pipe insert seat of embodiment 3 and the longitudinal sectional view of the mode heat transfer media plate that contacts with it.
Fig. 5 a is the PCR pipe insert seat of embodiment 4 and the structural representation of the mode heat transfer media plate that contacts with it;
Fig. 5 b is the PCR pipe insert seat and the longitudinal sectional view of the mode heat transfer media plate that contacts with it perpendicular to travel direction of embodiment 4.
Among the figure:
1-mode heat transfer media plate, 2-framework, 3-weighting material, 4-track, 5-casing chassis
6-specimen holder, 7-sample hose, 8-motor, 9-thermal source 3-1-aluminum weighting material
3-2-insulation filling materials 10-threaded hole 11-screw rod 12-PCR pipe insert seat
Embodiment
Below in conjunction with accompanying drawing, further describe the present invention by embodiment, but the scope that does not limit the present invention in any way.
PCR instrument shown in Fig. 1 a and 1b, three mode heat transfer media plates 1 are arranged, arrange in line, be fixed in the framework 2, mode heat transfer media plate 1 is simple rectangular block, adopt the material that it is good that aluminium or other conduct heat to make, the upper surface that 3, three mode heat transfer media plates 1 of solid filler and weighting material 3 are inserted in the space between per two adjacent platess forms a plane.Framework 2, mode heat transfer media plate 1 and weighting material 3 constitute a machinery integral, and this integral body is placed on the linear track 4, are promoted by linear electric motors 8, thereby realize that straight line moves.Track 4 is fixed on the casing chassis 5, and specimen holder 6 also is a fixed, and sample hose 7 is flat, be placed on the specimen holder 6 after, sample is with respect to the casing stationkeeping.Like this, linear electric motors 8 follow procedures move to specified working plate (being first mode heat transfer media plate among Fig. 1 a, is second mode heat transfer media plate among Fig. 1 b) under the specimen holder, realize the temperature of the change sample of program.
Concrete, as shown in Figure 2, the specimen holder 6 required areas that occupy be 36 * 54 millimeters, and mode heat transfer media plate 1 is 52 * 70 millimeters.Like this, even the out of true location has positive and negative 4 millimeters error that all samples is all placed on the mode heat transfer media plate, and still has 4 mm distance at least with the mode heat transfer media plate edge.Usually, the temperature of plate margin departs from the set-point easily, so in order to ensure the tolerance range of sample temperature, sample should not be too near to the edge.All closely by a thermal source 9 is housed, thermal source is a resistance wire formula heating unit under each mode heat transfer media plate.The thickness of mode heat transfer media plate 1 can be 5-40 millimeters, if thin excessively, not only physical strength is not enough, and thermal inertia is also not enough.Change the temperature of mode heat transfer media plate because can sop up a part of heat energy after sample is laid out, so if the mode heat transfer media plate thermal inertia is less, it is more that temperature can depart from preset value.But the blocked up meeting of mode heat transfer media plate strengthens machinery wieght.Gap between the mode heat transfer media plate is the 2-20 millimeter.The gap is too little, and adjacent two interplate thermal conduction meetings make the temperature of plate be not easy control; The gap is too big, and the temperature of plate interspace is not easy to maintain between the temperature of two plates, makes when sample slides between two plates, and temperature can excessive descent.Weighting material 3 between the mode heat transfer media plate 1 will play following effect: the first, and it and two plates form a plane, and making sample slide into another plate from a plate does not have mechanical obstacles; The second, it plays certain heat-insulation and heat-preservation effect, makes two adjacent media plates design temperature independently, and the temperature effect that prevents adjacent plate is to the temperature maintenance of self plate, and this insulation effect has also reduced the energy consumption of PCR instrument; The 3rd, between two plates, form the transition of a temperature, when sample when a plate slides into another plate, the temperature that it contacted also is the temperature gradual change from the temperature of previous plate towards a back plate.Distance between this example mesoplate is about 5 millimeters, and weighting material is a composite structure, and the material on its upper strata is identical with mode heat transfer media plate, aluminum weighting material 3-1 as shown in Figure 2 is about 1 mm thick, and lower floor is insulation filling materials 3-2, adopt thermal insulating material, as heat-stable porous plastics.This composite structure, its top is easy to realize its surface temperature between two media plates with aluminium block, and its mechanical property is consistent with mode heat transfer media plate, can avoid because of being uneven that wearing and tearing cause; The thermal insulating material of its underpart has reduced interplate thermal conduction again.In addition, a lid that can heat is arranged on specimen holder, in the PCR process, the temperature of this lid is about 100 ℃, to prevent condensing upon the sample hose top after water from dividing evaporation.This heating is covered with himself weight the PCR pipe is pressed on the media plate, makes the PCR pipe with mode heat transfer media plate excellent contact be arranged.Because the heating lid is a prior art, omits in the diagram.
This PCR instrument is arranged on annealing temperature with first mode heat transfer media plate in use, such as 55 ℃; Second mode heat transfer media plate is arranged on elongating temperature, such as 72 ℃; The 3rd mode heat transfer media plate is arranged on denaturation temperature, such as 94 ℃.After being placed on sample on the specimen holder 6, motor 8 places the 3rd mode heat transfer media plate under the specimen holder; Through several seconds, after sample temperature arrived denaturation temperature, motor 8 promoted the machinery integral slip that framework 2, mode heat transfer media plate 1 and weighting material 3 constitute, and first mode heat transfer media plate is under the specimen holder (sees Fig. 1 a); Pass through several seconds again, after sample temperature arrived annealing temperature, motor promoted machinery integral and slides, and second mode heat transfer media plate is under the specimen holder (sees Fig. 1 b); Pass through several seconds again, sample temperature arrives elongating temperature and continues one section specified time (being decided by concrete PCR reaction) promptly finishes a circulation.Motor promotes the machinery integral slip more then, the 3rd mode heat transfer media plate is placed under the specimen holder the next circulation of beginning.
As shown in Figure 3, the characteristics of this embodiment are that mode heat transfer media plate 1 is fixed on the casing, and sample is placed on the specimen holder 6, and specimen holder 6 is movably.Identical with embodiment 1, three mode heat transfer media plates 1 are arranged, thermal source is a resistance wire formula heating unit, is installed under the plate closely.Mode heat transfer media plate adopts the material that it is good that aluminium or other conduct heat.Mode heat transfer media plate is a simple rectangular block.Solid is inserted in space between per two adjacent media plates.Only from geometric angle, the upper surface of three mode heat transfer media plates 1 and weighting material 3 forms a plane.Three mode heat transfer media plates 1 are arranged in line, be fixed on the framework 2 and charges together, form a machinery integral, be fixed on the casing chassis 5.Specimen holder 6 is placed on the linear track 4.In this example, linear track 4 is that edge by framework 2 constitutes.One side of specimen holder 6 has a threaded hole 10, passes a screw rod 11 in the threaded hole 10, rotates this screw rod by a motor 8, thereby the linearity that realizes specimen holder 6 moves.The bottom of sample hose is the plane, is placed in by specimen holder 6 on the plane of mode heat transfer media plate 1.Like this, follow procedure moves to specimen holder 6 on the specified mode heat transfer media plate, realizes the temperature of the change sample of program.
The size of specimen holder 6 and mode heat transfer media plate 1, and the weighting material between the heat catalysis media plate is described identical with embodiment 1.
During use, first mode heat transfer media plate is arranged on annealing temperature, such as 55 ℃; Second mode heat transfer media plate is arranged on elongating temperature, such as 72 ℃; The 3rd mode heat transfer media plate is arranged on denaturation temperature, such as 94 ℃.After being placed on sample on the specimen holder 6, motor 8 places specimen holder 6 on the 3rd mode heat transfer media plate.Through several seconds, after sample temperature arrived denaturation temperature, motor 8 promoted specimen holder 6 and moves, and specimen holder 6 is on first mode heat transfer media plate.Pass through several seconds again, after sample temperature arrived annealing temperature, motor 8 promoted specimen holder 6 and moves, and specimen holder 6 is on second mode heat transfer media plate.Pass through several seconds again, sample temperature arrives elongating temperature.(being come calmly by concrete PCR reaction requirement) promptly finishes a circulation through one specific extension period.Motor promotes specimen holder again and moves then, specimen holder is placed on the 3rd mode heat transfer media plate next circulation of beginning.
Above-mentioned two embodiment have adopted specially designed flat PCR sample hose, but this is not the condition an of necessity.In the present embodiment, the PCR pipe is general tapered tube, realizes thermocouple (thermal coupling) by the good PCR pipe insert seat of heat conduction and the mode heat transfer media plate of preheating.The selection of PCR pipe insert seat profile and material is similar to the media plate in first type of PCR instrument described in the background technology, as shown in Figs. 4a and 4b, the one side of PCR pipe insert seat 12 has the tapered hole that inserts the PCR pipe, and relative one side is one and mode heat transfer media plate 1 contacted plane.PCR pipe insert seat in this example is equivalent to the specimen holder among the embodiment 1, and all the other structures are identical with embodiment 1.Because the PCR pipe insert seat has a thermal inertia of can not ignore, after it touches the media plate, can absorb a heat of can not ignore, make the temperature departure preheating temperature of mode heat transfer media plate and sample hose.So the preheating temperature of mode heat transfer media plate should be made corresponding adjustment according to practical situation and be compensated the temperature deviation that the socket thermal inertia brings.Adjustment amount can be determined by calculating and testing.In this scheme, the heat energy that changes PCR pipe insert seat and sample hose temperature is to be provided by the heat energy that stores in the mode heat transfer media plate basically, and from this aspect, this PCR instrument is very different with first kind PCR instrument.The heat energy that first kind PCR instrument changes temperature is in the process of carrying out temperature change, is directly provided by cold/heat source, and required energy decides between being taken the opportunity by power, need the regular hour accumulation, so the speed of temperature change is slower.Because what utilize is the heat energy of laying in the mode heat transfer media plate warm, PCR pipe and socket temperature change speed depend on thermal conductivity in the present embodiment.The heat energy of preheating can be by a lower-powered well heater (or scatterer) by long period (whole thermal cycling) accumulation in the mode heat transfer media plate.
Embodiment 4
Identical with embodiment 3, standard universal PCR pipe forms thermocouple by a PCR pipe insert seat and mode heat transfer media plate, but different with embodiment 3 in configuration design at the mode heat transfer media plate contact surface.PCR pipe insert seat 12 is pruned part metals from the bottom through processing, between several rows of PCR pipe jack, form the strip groove of series of parallel, the trend of these strip grooves is consistent with the travel direction of mode heat transfer media plate 1, cross section perpendicular to travel direction is " △ " shape, corresponding, on mode heat transfer media plate 1, process a series of strip projected parts that matches with these strip groove surfaces, concrete structure is referring to Fig. 5 a and 5b, and the four-headed arrow among Fig. 5 a is represented the slip direction of mode heat transfer media plate 1.The advantage of this design is: 1. because after the part of pruning, PCR pipe insert seat volume reduces, thereby has reduced its thermal inertia; 2. strengthened the contact area of sample and mode heat transfer media plate; 3.PCR the surface of pipe and the surface distance of mode heat transfer media plate shorten.More than three characteristics can accelerate thermal conduction, PCR instrument lifting temperature efficiency is improved.In this embodiment, the volume of PCR pipe insert seat 12 is 15.0cm
3, the contact area of it and mode heat transfer media plate 1 is 64.1cm
2By contrast, if do not cut out strip groove, the volume of PCR pipe insert seat is 24.8cm
3, the contact area of it and mode heat transfer media plate is 19.4cm
2After cutting out strip groove, it is about 40% that volume has reduced, and contact area increases to three times.Simultaneously, cut out strip groove after, the distance that PCR manages mode heat transfer media plate also clearly shortens.
Claims (10)
1. PCR instrument, mode heat transfer media plate and a specimen holder that is used to place sample hose of comprising a casing, a plurality of predeterminable temperature, wherein: described a plurality of mode heat transfer media plates are arranged under the specimen holder, and their upper surface equates to the vertical range of specimen holder, under the effect of transmission mechanism, mode heat transfer media plate and specimen holder relatively sliding, sliding trace is on same plane, and sample hose contacts with the upper surface of the different heat transfer media plate that is preheating to differing temps indirectly or directly according to certain procedure in the sliding process.
2. PCR instrument as claimed in claim 1 is characterized in that: described a plurality of mode heat transfer media plates are arranged in a straight line or are arranged on the circular arc.
3. PCR instrument as claimed in claim 1 is characterized in that: described a plurality of mode heat transfer media plates are fixed in the framework, and solid filler is inserted in the space between the plate, and the upper surface of all mode heat transfer media plates and weighting material equates to the vertical range of specimen holder.
4. PCR instrument as claimed in claim 3 is characterized in that: described transmission mechanism is connected with the fixing framework of mode heat transfer media plate, perhaps is connected with specimen holder.
5. PCR instrument as claimed in claim 3, it is characterized in that: described mode heat transfer media plate adopts the good conductor of heat to make, and the weighting material that is filled between the plate adopts bilevel composite structure, and the material on upper strata is identical with mode heat transfer media plate, and lower floor adopts thermal insulating material.
6. PCR instrument as claimed in claim 1 is characterized in that: each mode heat transfer media plate all has a thermal source that it is carried out preheating, and thermal source is resistive heating elements or thermoelectric effect element.
7. PCR instrument as claimed in claim 1 is characterized in that: have a heating lid on the described specimen holder, in the PCR process, this heating lid is pressed on the sample hose.
8. as the described PCR instrument of arbitrary claim in the claim 1～7, it is characterized in that: the upper surface of described mode heat transfer media plate is the plane, described specimen holder is a flat board with holes, and the bottom of sample hose is the plane, after sample hose inserted specimen holder, the bottom of sample hose directly contacted with the upper surface of mode heat transfer media plate.
9. as the described PCR instrument of arbitrary claim in the claim 1～7, it is characterized in that: the upper surface of described mode heat transfer media plate is the plane, specimen holder then be one that make by the good conductor of heat, upper surface has PCR tapered tube jack, lower surface is a planar PCR pipe insert seat, the lower surface of PCR pipe insert seat contacts with the upper surface of mode heat transfer media plate.
10. as the described PCR instrument of arbitrary claim in the claim 1～7, it is characterized in that: described specimen holder is a PCR pipe insert seat that made by the good conductor of heat, that upper surface has PCR tapered tube jack, have the strip groove of series of parallel in its bottom, upper surface at mode heat transfer media plate also correspondingly has a series of strip projected parts that matches with these strip groove surfaces, and these strip grooves are consistent with the relatively sliding direction of mode heat transfer media plate and PCR pipe insert seat with the trend of projection.
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| FR2672231A1 (en) * | 1991-02-01 | 1992-08-07 | Eibet | Apparatus for repeated automatic execution of a heat cycle, especially for the amplification of the number of a defined nucleic acid sequence |
| CN2102296U (en) * | 1991-06-27 | 1992-04-22 | 华西医科大学 | Program controlled automatic circulation instrument for polymerase chain reaction |
| CN2199213Y (en) * | 1994-06-02 | 1995-05-31 | 北京市科海微控新技术联合开发部 | Four-water bath cell type gene augmentor |
| CN2258618Y (en) * | 1996-02-12 | 1997-07-30 | 中国人民解放军第四军医大学 | Sample trough for multi-function PCR gene amplification instrument |
| CN2697102Y (en) * | 2004-04-01 | 2005-05-04 | 中国人民解放军基因工程研究所 | Liquid flowing reaction constent-temp. box for PCR augmentor |
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