CN104962453A - Device for amplification and reaction of tube cap gene chip - Google Patents
Device for amplification and reaction of tube cap gene chip Download PDFInfo
- Publication number
- CN104962453A CN104962453A CN201510304625.1A CN201510304625A CN104962453A CN 104962453 A CN104962453 A CN 104962453A CN 201510304625 A CN201510304625 A CN 201510304625A CN 104962453 A CN104962453 A CN 104962453A
- Authority
- CN
- China
- Prior art keywords
- reaction chamber
- described reaction
- cover plate
- pivoted frame
- automatically
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
Abstract
The invention discloses a device for amplification and reaction of a tube cap gene chip. The device comprises a closed cavity composed of a reaction cavity cover plate, a reaction cavity automatic door and a reaction cavity outer barrel; a reaction cavity pipe is arranged above the reaction cavity cover plate, and a rotation support and a reaction cup support arranged above the rotation support are arranged in the reaction cavity outer barrel; the rotation support is vertically connected with a rotation support rotation shaft, one part of the rotation support rotation shaft is located at the bottom of the reaction cavity outer barrel, and a first synchronous pulley is arranged at the bottom end of the structure, located at the bottom of the reaction cavity outer barrel, of the rotation support rotation shaft; a reaction cavity rotation motor support, a reaction cavity rotation motor connected with the reaction cavity rotation motor support and a second synchronous pulley arranged at the bottom end of the reaction cavity rotation motor are arranged at the bottom of the reaction cavity outer barrel; and the first synchronous pulley is in transmission connection with the second synchronous pulley through a synchronous pulley. Full-process automation is achieved, manual turning of a sample tube is not needed, the detection period is saved, and the reaction completeness is improved.
Description
Technical field
The invention belongs to pipe gai gene chip amplified reaction technical field, specifically relate to a kind of device increasing for pipe gai gene chip and react.
Background technology
At present about the amplification of pipe gai gene chip and reaction technology, domestic and international existing conventional techniques, normally adopt manual operation, to end from reaction, all need by manual operation or use robotic arm manipulation to complete, but user needs certain professional skill, complicated operation and excessive cycle, in operating process, owing to adopting manual hand manipulation, the temperature variation of reaction is too large affects measuring result and cost is too high.
Therefore, need to propose a kind of novel increasing and reaction unit for pipe gai gene chip.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the invention provides a kind of device increasing for pipe gai gene chip and react.
Technical scheme: for achieving the above object, a kind of device increasing for pipe gai gene chip and react of the present invention, comprise reaction chamber cover plate, reaction chamber automatically-controlled door and reaction chamber urceolus, opening is provided with above described reaction chamber cover plate, described reaction chamber automatically-controlled door is located at above described reaction chamber cover plate for covering the opening on described reaction chamber cover plate, described reaction chamber urceolus is located at below described reaction chamber cover plate, described reaction chamber urceolus is positioned at immediately below described reaction chamber automatically-controlled door, reaction chamber pipeline is provided with above described reaction chamber cover plate, be provided with pivoted frame in described reaction chamber urceolus and be located at the reaction glass stand above described pivoted frame, described pivoted frame vertically connects pivoted frame rotating shaft, described some structure of pivoted frame rotating shaft is positioned at the bottom of described reaction chamber urceolus, the bottom of the structure that described pivoted frame rotating shaft is positioned at bottom described reaction chamber urceolus is provided with the first synchronous pulley, the bottom of described reaction chamber urceolus is provided with reaction chamber rotating machine support, be positioned at described reaction chamber rotating machine support side and the reaction chamber rotating machine be connected with described reaction chamber rotating machine support and the second synchronous pulley being located at described reaction chamber rotating machine bottom, described first synchronous pulley is connected with described second synchronous belt pulley transmission by Timing Belt.
Further, be also provided with reaction chamber automatic door motor and automatically-controlled door position transducer above described reaction chamber cover plate, described reaction chamber automatic door motor is positioned at described reaction chamber automatically-controlled door side and is connected with described reaction chamber automatically-controlled door.
Further, the quantity of described reaction chamber pipeline is 4, and 4 reaction chamber pipelines lay respectively at 4 ports of described reaction chamber cover plate.
Further, the axis of described pivoted frame rotating shaft overlaps with the neutrality line of described pivoted frame.
Further, form airtight cavity by described reaction chamber cover plate, reaction chamber automatically-controlled door and reaction chamber urceolus, described airtight cavity inwall is provided with heat keeper.
Further, described reaction glass stand comprises reaction cup stand and reaction cup compact heap, evenly be provided with the cylindrical reaction chamber that 8 volumes are identical above described reaction cup stand, described reaction cup compact heap is located at described reaction cup stand one end and for compressing 8 cylindrical reaction chambers.
Beneficial effect: compared with the prior art, the advantage had is: whole process realizes automatization in design of the present invention, overturning sample hose without the need to manually going, saving sense cycle, improve the thoroughness of reaction.
Accompanying drawing explanation
Fig. 1 is the structural representation of the device increased for pipe gai gene chip and react of the present invention.
Fig. 2 is the sectional view of Fig. 1.
Fig. 3 is the structural representation of reaction glass stand.
Embodiment
Below in conjunction with embodiment, the present invention is further described.
See figures.1.and.2, a kind of device increasing for pipe gai gene chip and react provided by the invention, comprise the airtight cavity be made up of described reaction chamber cover plate 1, reaction chamber automatically-controlled door 4 and reaction chamber urceolus 15, described airtight cavity inwall is provided with heat keeper 14;
Opening is provided with above wherein said reaction chamber cover plate 1, described reaction chamber automatically-controlled door 4 is located at above described reaction chamber cover plate 1 for covering the opening on described reaction chamber cover plate 1, described reaction chamber urceolus 15 is located at below described reaction chamber cover plate 1, and described reaction chamber urceolus 15 is positioned at immediately below described reaction chamber automatically-controlled door 4, described reaction chamber automatically-controlled door 4 is identical with described openings of sizes just in time to be sealed described opening, described reaction chamber automatically-controlled door 4 can open and close automatically, reaction chamber automatic door motor 3 and automatically-controlled door position transducer 5 is also provided with above described reaction chamber cover plate 1, described reaction chamber automatic door motor 3 is positioned at described reaction chamber automatically-controlled door 4 side and is connected with described reaction chamber automatically-controlled door 4, described reaction chamber automatic door motor 3 is for providing electric energy for described reaction chamber automatically-controlled door 4, the junction position of described automatically-controlled door position transducer 5 between described reaction chamber automatically-controlled door 4 and described reaction chamber cover plate 1, described automatically-controlled door position transducer 5 is same as the location status detecting described reaction chamber automatically-controlled door 4, reaction chamber pipeline 2 is provided with above described reaction chamber cover plate 1, the quantity of described reaction chamber pipeline 2 is 4, 4 reaction chamber pipelines 2 lay respectively at 4 ports of described reaction chamber cover plate 1, the reaction glass stand 6 being provided with pivoted frame 7 in described reaction chamber urceolus 15 and being located at above described pivoted frame 7, described pivoted frame 7 vertically connects pivoted frame rotating shaft 8, the axis of described pivoted frame rotating shaft 8 overlaps with the neutrality line of described pivoted frame 7, namely the top of described pivoted frame rotating shaft 8 is just in time positioned at the point midway of described pivoted frame 7, described some structure of pivoted frame rotating shaft 8 is positioned at the bottom of described reaction chamber urceolus 15, the bottom of the structure that described pivoted frame rotating shaft 8 is positioned at bottom described reaction chamber urceolus 15 is provided with the first synchronous pulley 12, the bottom of described reaction chamber urceolus 15 is provided with reaction chamber rotating machine support 8, be positioned at described reaction chamber rotating machine support 8 side and the reaction chamber rotating machine 9 be connected with described reaction chamber rotating machine support 8 and the second synchronous pulley 10 being located at described reaction chamber rotating machine 9 bottom, described first synchronous pulley 12 is in transmission connection by Timing Belt 11 and described second synchronous pulley 10,
With reference to Fig. 3, described reaction glass stand 6 comprises reaction cup stand 16 and reaction cup compact heap 17, evenly be provided with the cylindrical reaction chamber that 8 volumes are identical above described reaction cup stand 16, described reaction cup compact heap 17 is located at described reaction cup stand 16 one end and for compressing 8 cylindrical reaction chambers;
The principle of work of the device increased for pipe gai gene chip and react of the present invention:
First, sample is put into the cylindrical reaction chamber on reaction glass stand 6, then the reaction glass stand 6 be located at above pivoted frame 7 is arranged on pivoted frame 7, can rotate along with pivoted frame 7 by realization response glass stand 6;
Then, reaction chamber automatic door motor 3 quits work and reaction chamber automatically-controlled door 4 is closed automatically;
Then reaction chamber rotating machine 9 starts to rotate thus drives the second synchronous pulley 10 being located at reaction chamber rotating machine 9 bottom to rotate, because the first synchronous pulley 12 is in transmission connection by Timing Belt 11 and the second synchronous pulley 10, so the first synchronous pulley 12 rotates along with the rotation of the second synchronous pulley 10;
The reaction chamber pipeline 2 be located at above reaction chamber cover plate 1 supplies constant stable air by other equipment, and temperature can set as required, high temperature gas flow is passed to sample by reaction chamber pipeline 2, because sample is at airtight cavity internal rotating, so sample can thermally equivalent, according to the difference of temperature setting, the change of sample self-temperature can be realized;
Then after some circulations, reaction chamber automatic door motor 3 is started working and reaction chamber automatically-controlled door 4 is beaten automatically, now by other equipment, sample is carried out upset and makes sample fully carry out by thermal response; Finally when respond terminate after, automatically by sample take out, whole process be all by time variable control realize automatization load and reaction.
The above is only the preferred embodiment of the present invention; be noted that for those skilled in the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. the device that can increase for pipe gai gene chip and react, it is characterized in that: comprise reaction chamber cover plate (1), reaction chamber automatically-controlled door (4) and reaction chamber urceolus (15), described reaction chamber cover plate (1) top is provided with opening, described reaction chamber automatically-controlled door (4) is located at described reaction chamber cover plate (1) top and is used for covering the opening on described reaction chamber cover plate (1), described reaction chamber urceolus (15) is located at described reaction chamber cover plate (1) below, described reaction chamber urceolus (15) is positioned at immediately below described reaction chamber automatically-controlled door (4), described reaction chamber cover plate (1) top is provided with reaction chamber pipeline (2), be provided with pivoted frame (7) in described reaction chamber urceolus (15) and be located at the reaction glass stand (6) above described pivoted frame (7), described pivoted frame (7) vertically connects pivoted frame rotating shaft (8), described some structure of pivoted frame rotating shaft (8) is positioned at the bottom of described reaction chamber urceolus (15), the bottom that described pivoted frame rotating shaft (8) is positioned at the structure of described reaction chamber urceolus (15) bottom is provided with the first synchronous pulley (12), the bottom of described reaction chamber urceolus (15) is provided with reaction chamber rotating machine support (8), be positioned at described reaction chamber rotating machine support (8) side and the reaction chamber rotating machine (9) be connected with described reaction chamber rotating machine support (8) and the second synchronous pulley (10) being located at described reaction chamber rotating machine (9) bottom, described first synchronous pulley (12) is in transmission connection by Timing Belt (11) and described second synchronous pulley (10).
2. the device increasing for pipe gai gene chip and react according to claim 1, it is characterized in that: described reaction chamber cover plate (1) top is also provided with reaction chamber automatic door motor (3) and automatically-controlled door position transducer (5), and described reaction chamber automatic door motor (3) is positioned at described reaction chamber automatically-controlled door (4) side and is connected with described reaction chamber automatically-controlled door (4).
3. the device increasing for pipe gai gene chip and react according to claim 1, it is characterized in that: the quantity of described reaction chamber pipeline (2) is 4,4 reaction chamber pipelines (2) lay respectively at 4 ports of described reaction chamber cover plate (1).
4. the device increasing for pipe gai gene chip and react according to claim 1, is characterized in that: the axis of described pivoted frame rotating shaft (8) overlaps with the neutrality line of described pivoted frame (7).
5. the device increasing for pipe gai gene chip and react according to claim 1, it is characterized in that: form airtight cavity by described reaction chamber cover plate (1), reaction chamber automatically-controlled door (4) and reaction chamber urceolus (15), described airtight cavity inwall is provided with heat keeper (14).
6. the device increasing for pipe gai gene chip and react according to claim 1, it is characterized in that: described reaction glass stand (6) comprises reaction cup stand (16) and reaction cup compact heap (17), described reaction cup stand (16) top is evenly provided with the identical cylindrical reaction chamber of 8 volumes, and described reaction cup compact heap (17) is located at described reaction cup stand (16) one end and for compressing 8 cylindrical reaction chambers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510304625.1A CN104962453A (en) | 2015-06-04 | 2015-06-04 | Device for amplification and reaction of tube cap gene chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510304625.1A CN104962453A (en) | 2015-06-04 | 2015-06-04 | Device for amplification and reaction of tube cap gene chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104962453A true CN104962453A (en) | 2015-10-07 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510304625.1A Pending CN104962453A (en) | 2015-06-04 | 2015-06-04 | Device for amplification and reaction of tube cap gene chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104962453A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105936869A (en) * | 2016-06-23 | 2016-09-14 | 浙江爱易生物医学科技有限公司 | Multifunctional fluorescent quantitative PCR instrument |
-
2015
- 2015-06-04 CN CN201510304625.1A patent/CN104962453A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105936869A (en) * | 2016-06-23 | 2016-09-14 | 浙江爱易生物医学科技有限公司 | Multifunctional fluorescent quantitative PCR instrument |
| CN105936869B (en) * | 2016-06-23 | 2018-05-25 | 浙江爱易生物医学科技有限公司 | A kind of Multifunction fluorescent quantitative PCR apparatus |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151007 |