CN106479860B - A kind of self-sealing fluorescence quantitative PCR instrument - Google Patents
A kind of self-sealing fluorescence quantitative PCR instrument Download PDFInfo
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- CN106479860B CN106479860B CN201610894582.1A CN201610894582A CN106479860B CN 106479860 B CN106479860 B CN 106479860B CN 201610894582 A CN201610894582 A CN 201610894582A CN 106479860 B CN106479860 B CN 106479860B
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- cylinder
- pcr instrument
- instrument host
- electromagnet
- hollow out
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
Abstract
A kind of self-sealing fluorescence quantitative PCR instrument, belong to technical field of medical equipment, including flip lid, first cylinder, pressing plate, first electromagnet, multistage retractable rod, second electromagnet, controller, PCR instrument host, rack for test tube, second cylinder, hyaline membrane, sucker, fagging, first motor, roller, tub, scissor arms, first supporting plate, first hollow out, first pin shaft, cutter, third cylinder, linear motor, movable block, 4th cylinder, second supporting plate, second hollow out and the second pin shaft, flip lid is installed on the PCR instrument host, rack for test tube is installed in PCR instrument host, right side wall installs two the first supporting plates in PCR instrument host, first supporting plate is equipped with the first hollow out, two the first pin shafts are installed in the first hollow out, the first pin shaft being located above is connected with the piston rod of third cylinder, third cylinder is mounted on P Right side wall in CR instrument host；The invention has the advantages that having the function of automatic sealing.
Description
Technical field
The present invention relates to a kind of self-sealing fluorescence quantitative PCR instruments, belong to technical field of medical equipment.
Background technique
Real time fluorescent quantitative pcr instrument, is made of fluorescent quantitation system and computer, for monitoring the fluorescence of cyclic process.
The computer being connected with real-time device collects fluorescence data.Data are graphically shown by the real-time analysis software of exploitation
Show.Initial data is drawn into chart of the fluorescence intensity relative to recurring number.It can start to analyze after source data collection.In real time
The software of equipment can make the data being collected into carry out normalization processing to make up the difference of background fluorescence.The fluorescence of the prior art is fixed
Amount PCR instrument does not all have the function of sealing test tube automatically, and test tube is put into fluorescent quantitation again after needing experimenter to seal manually
In PCR instrument, use pretty troublesome.In order to solve the above difficulties, need to develop a self-sealing fluorescence quantitative PCR instrument.
Summary of the invention
The object of the present invention is to provide a kind of self-sealing fluorescence quantitative PCR instruments.
The problem to be solved in the present invention is that the fluorescence quantitative PCR instrument of the prior art does not all have the function of sealing test tube automatically
The problem of.
To achieve the purpose of the present invention, the technical solution adopted by the present invention is that:
A kind of self-sealing fluorescence quantitative PCR instrument, including flip lid, the first cylinder, pressing plate, the first electromagnet, multistage are stretched
Contracting bar, the second electromagnet, controller, PCR instrument host, rack for test tube, the second cylinder, hyaline membrane, sucker, fagging, first motor, rolling
Cylinder, tub, scissor arms, the first supporting plate, the first hollow out, the first pin shaft, cutter, third cylinder, linear motor, movable block, the 4th
Cylinder, the second supporting plate, the second hollow out and the second pin shaft install flip lid on the PCR instrument host, install test tube in PCR instrument host
Frame, right side wall installs two the first supporting plates in PCR instrument host, and the first supporting plate is equipped with the first hollow out, installs two in the first hollow out
A first pin shaft, the first pin shaft being located above are connected with the piston rod of third cylinder, and third cylinder is mounted in PCR instrument host
Right side wall, scissor arms right end are respectively mounted in the first hollow out two sides up and down, and two sides are respectively mounted on two the up and down for scissor arms left end
On two pin shafts, the second pin shaft is mounted in the second hollow out, and the second supporting plate is equipped with the second hollow out, installation second on the left of the second supporting plate
Electromagnet, left side wall installs multistage retractable rod in PCR instrument host, and the first electromagnet, the first electromagnetism are installed in multistage retractable rod front end
Iron and the second electromagnet are in same level, and the second cylinder is installed under the second supporting plate, and sucker, the second gas are installed under the second cylinder
Cylinder right is equipped with roller, and roller is mounted in tub, and tub is mounted in PCR instrument host on right side wall, and the axis of roller passes through connection
Axis device is connected with first motor, and first motor is mounted in tub, and fagging is installed in PCR instrument host, and fagging is located at below tub,
PCR instrument host inner top installs two the first cylinders, and pressing plate is installed under the first cylinder, and PCR instrument host inner top installs straight-line electric
Machine installs movable block on linear motor, the 4th cylinder is installed on movable block, installs cutter under the 4th cylinder, controller is mounted on
On the left of PCR instrument host, controller by conducting wire respectively with first motor, linear motor, multistage retractable rod, the first electromagnet and the
Two electromagnet are connected.
First solenoid valve built in first cylinder, the first solenoid valve are connected by conducting wire with controller.
Second solenoid valve built in second cylinder, second solenoid valve are connected by conducting wire with controller.
4th solenoid valve built in 4th cylinder, the 4th solenoid valve are connected by conducting wire with controller.
The invention has the advantages that opening flip lid, test tube to be put on rack for test tube, controller controls first motor starting,
Roller is rotated, hyaline membrane is loosened；Controller controls the second cylinder and does stretching motion, moves down sucker, inhales sucker
Attached firmly hyaline membrane；Controller control third cylinder do stretching motion, extend scissor arms, make sucker with hyaline membrane forward
It is mobile, so that the second electromagnet is touched the first electromagnet；Controller controls magnetic phase after the first electromagnet and the energization of the second electromagnet
It inhales；Controller controls the first cylinder and does stretching motion, moves down pressing plate, is pressed in pressing plate on test tube hyaline membrane；Control
Device controls the second cylinder and does stretching motion, moves down cutter；Controller controls linear motor starting, moves movable block or so
It is dynamic, so that cutter is cut hyaline membrane.
Detailed description of the invention
Fig. 1 is a kind of self-sealing fluorescence quantitative PCR instrument overall structure figure of the present invention；
Fig. 2 is the top view of Linear motor mounting structure；
Fig. 3 is the left view of the second cylinder mounting structure；
In figure: 1, renovating the 2, first cylinder 3, pressing plate 4, the first electromagnet 5, the 6, second electricity of multistage retractable rod
Magnet 7, controller 8, PCR instrument host 9, rack for test tube 10, the second cylinder 11, hyaline membrane 12, sucker 13, support
Plate 14, first motor 15, roller 16, tub 17, scissor arms 18, the first supporting plate 19, the first hollow out 20,
One pin shaft 21, cutter 22, third cylinder 23, linear motor 24, movable block 25,26, second, the 4th cylinder
Plate 27, the second hollow out 28, the second pin shaft.
Specific embodiment
The present invention is further illustrated with reference to the accompanying drawings and embodiments.
A kind of self-sealing fluorescence quantitative PCR instrument of the present invention, including flip lid the 1, first cylinder 2, pressing plate 3, the first electromagnetism
Iron 4, the second electromagnet 6, controller 7, PCR instrument host 8, rack for test tube 9, the second cylinder 10, hyaline membrane 11, is inhaled multistage retractable rod 5
Disk 12, fagging 13, first motor 14, roller 15, tub 16, scissor arms 17, the first supporting plate 18, the first hollow out 19, the first pin shaft
20, cutter 21, third cylinder 22, linear motor 23, movable block 24, the 4th cylinder 25, the second supporting plate 26, the second hollow out 27 and
Two pin shafts 28, installation flip lid 1 on the PCR instrument host 8 install rack for test tube 9, right side wall in PCR instrument host 8 in PCR instrument host 8
Two the first supporting plates 18 are installed, the first supporting plate 18 is equipped with the first hollow out 19, installs two the first pin shafts 20 in the first hollow out 19,
The first pin shaft 20 being located above is connected with the piston rod of third cylinder 22, and third cylinder 22 is mounted on right side in PCR instrument host 8
Wall, controller 7 control third cylinder 22 do stretching motion, extend scissor arms 17, make sucker 12 with hyaline membrane 11 to
Preceding movement makes the second electromagnet 6 touch the first electromagnet 4；17 right end of scissor arms is respectively mounted in the first hollow out 19 two sides up and down,
17 left end of scissor arms is respectively mounted on two the second pin shafts 28 two sides up and down, and the second pin shaft 28 is mounted in the second hollow out 27, the
Two supporting plates 26 are equipped with the second hollow out 27, install the second electromagnet 6 on the left of the second supporting plate 26, and left side wall is installed in PCR instrument host 8
The first electromagnet 4 is installed in multistage retractable rod 5,5 front end of multistage retractable rod, and the first electromagnet 4 and the second electromagnet 6 are in same water
Plane, magnetism is attracting after controller 7 controls the first electromagnet 4 and the energization of the second electromagnet 6；Second supporting plate 26 is lower to install the second gas
Cylinder 10, second solenoid valve built in the second cylinder 10, second solenoid valve are connected by conducting wire with controller 7, the control of controller 7 second
Cylinder 10 does stretching motion, moves down sucker 12, and sucker 12 is made to adsorb hyaline membrane 11；Controller 7 controls the second cylinder
10 do stretching motion, move down cutter 21；The lower installation sucker 12 of second cylinder 10,10 right of the second cylinder is equipped with roller
15, roller 15 is mounted in tub 16, and on roller 15,11 other end of hyaline membrane is pasted to be inhaled 11 one end fixing wound of hyaline membrane
Under disk 12, hyaline membrane and sucker 12 are attached together, and tub 16 is mounted in PCR instrument host 8 on right side wall, and the axis of roller 15 is logical
It crosses shaft coupling to be connected with first motor 14, first motor 14 is mounted in tub 16, opens flip lid 1, test tube is put into test tube
On frame 9, controller 7 controls first motor 14 and starts, and rotates roller 15, loosens hyaline membrane 11；Installation in PCR instrument host 8
Fagging 13, fagging 13 are located at the lower section of tub 16, and 8 inner top of PCR instrument host installs two the first cylinders 2, the built in the first cylinder 2
One solenoid valve, the first solenoid valve are connected by conducting wire with controller 7, and controller 7 controls the first cylinder 2 and does stretching motion, make to press
Plate 3 moves down, and is pressed in pressing plate 3 on test tube hyaline membrane 11；The lower installation pressing plate 3 of first cylinder 2,8 inner top of PCR instrument host
Linear motor 23 is installed, movable block 24 is installed on linear motor 23, controller 7 controls linear motor 23 and starts, and makes movable block 24
It moves left and right, cutter 21 is made to cut hyaline membrane 11, the 4th cylinder 25 is installed on movable block 24, the 4th built in the 4th cylinder 25
Solenoid valve, the 4th solenoid valve are connected by conducting wire with controller 7, and the lower installation cutter 21 of the 4th cylinder 25, controller 7 is mounted on
8 left side of PCR instrument host, controller 7 are electric with first motor 14, linear motor 23, multistage retractable rod 5, first respectively by conducting wire
Magnet 4 is connected with the second electromagnet 6.
Application method of the present invention: opening flip lid 1, test tube be put on rack for test tube 9, and controller 7 controls first motor 14
Starting rotates roller 15, loosens hyaline membrane 11；Controller 7 controls the second cylinder 10 and does stretching motion, keeps sucker 12 downward
It is mobile, so that sucker 12 is adsorbed hyaline membrane 11；Controller 7 controls third cylinder 22 and does stretching motion, and scissor arms 17 is made to trail
Come, moves forward sucker 12 with hyaline membrane 11, the second electromagnet 6 is made to touch the first electromagnet 4；The control of controller 7 first
Electromagnet 4 and the second electromagnet 6 are magnetic attracting after being powered；Controller 7 controls the first cylinder 2 and does stretching motion, keeps pressing plate 3 downward
It is mobile, be pressed in pressing plate 3 hyaline membrane 11 on test tube；Controller 7 controls the second cylinder 10 and does stretching motion, keeps cutter 21 downward
It is mobile；Controller 7 controls linear motor 23 and starts, and moves left and right movable block 24, and cutter 21 is made to cut hyaline membrane 11.
Claims (1)
1. a kind of self-sealing fluorescence quantitative PCR instrument, including flip lid (1), the first cylinder (2), pressing plate (3), the first electromagnet
(4), multistage retractable rod (5), the second electromagnet (6), controller (7), PCR instrument host (8), rack for test tube (9), the second cylinder
(10), hyaline membrane (11), sucker (12), fagging (13), first motor (14), roller (15), tub (16), scissor arms (17),
First supporting plate (18), the first pin shaft (20), cutter (21), third cylinder (22), linear motor (23), moves at the first hollow out (19)
Motion block (24), the 4th cylinder (25), the second supporting plate (26), the second hollow out (27) and the second pin shaft (28), it is characterized in that: described
Installation flip lid (1) on PCR instrument host (8), PCR instrument host (8) is interior to install rack for test tube (9), the interior right side wall peace of PCR instrument host (8)
Two the first supporting plates (18) are filled, the first supporting plate (18) is equipped with the first hollow out (19), and two first are installed in the first hollow out (19)
Pin shaft (20), the first pin shaft (20) being located above are connected with the piston rod of third cylinder (22), and third cylinder (22) is mounted on
PCR instrument host (8) interior right side wall, scissor arms (17) right end are respectively mounted on the first hollow out (19) two sides up and down, scissor arms (17)
Left end is respectively mounted on two the second pin shafts (28) two sides up and down, and the second pin shaft (28) is mounted on the second hollow out (27), and second
Supporting plate (26) is equipped with the second hollow out (27), installs the second electromagnet (6) on the left of the second supporting plate (26), left in PCR instrument host (8)
Side wall installs multistage retractable rod (5), and the first electromagnet (4) are installed in multistage retractable rod (5) front end, the first electromagnet (4) and second
Electromagnet (6) is in same level, installs under the second supporting plate (26) the second cylinder (10), and the second cylinder installs sucker under (10)
(12), the second cylinder (10) right is equipped with roller (15), and (15 are mounted on tub (16) roller, hyaline membrane (11) and sucker
(12) be attached together, tub (16) is mounted on PCR instrument host (8) interior right side wall, the axis of roller (15) by shaft coupling with
First motor (14) is connected, and first motor (14) is mounted on tub (16), installs fagging (13) in PCR instrument host (8), fagging
(13) it is located at below tub (16), PCR instrument host (8) inner top installs two the first cylinders (2), and the first cylinder is installed under (2)
Pressing plate (3), PCR instrument host (8) inner top install linear motor (23), install movable block (24) on linear motor (23), mobile
4th cylinder (25) is installed on block (24), cutter (21) are installed under the 4th cylinder (25), controller (7) is mounted on PCR instrument host
(8) left side, controller (7) by conducting wire respectively with first motor (14), linear motor (23), multistage retractable rod (5), first electricity
Magnet (4) is connected with the second electromagnet (6), the first solenoid valve built in the first cylinder (2), and the first solenoid valve passes through conducting wire and control
Device (7) is connected, second solenoid valve built in the second cylinder (10), and second solenoid valve is connected by conducting wire with controller (7), the 4th gas
4th solenoid valve built in cylinder (25), the 4th solenoid valve are connected by conducting wire with controller (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610894582.1A CN106479860B (en) | 2016-10-14 | 2016-10-14 | A kind of self-sealing fluorescence quantitative PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610894582.1A CN106479860B (en) | 2016-10-14 | 2016-10-14 | A kind of self-sealing fluorescence quantitative PCR instrument |
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| Publication Number | Publication Date |
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| CN106479860A CN106479860A (en) | 2017-03-08 |
| CN106479860B true CN106479860B (en) | 2019-10-15 |
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| CN201610894582.1A Active CN106479860B (en) | 2016-10-14 | 2016-10-14 | A kind of self-sealing fluorescence quantitative PCR instrument |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107311089B (en) * | 2017-08-04 | 2023-12-22 | 三维医疗科技江苏股份有限公司 | Miniaturized medical test tube sealing instrument |
| CN108048315B (en) * | 2018-01-04 | 2021-01-19 | 深圳大学 | Fluorescent quantitative PCR instrument based on full-automatic sample adding |
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| CN105936869A (en) * | 2016-06-23 | 2016-09-14 | 浙江爱易生物医学科技有限公司 | Multifunctional fluorescent quantitative PCR instrument |
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| US5269918A (en) * | 1990-07-09 | 1993-12-14 | Cytyc Corporation | Clinical cartridge apparatus |
| US7169355B1 (en) * | 2000-02-02 | 2007-01-30 | Applera Corporation | Apparatus and method for ejecting sample well trays |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101668590A (en) * | 2007-04-25 | 2010-03-10 | 3M创新有限公司 | Chemical constituent and processing device assembly |
| CN201447340U (en) * | 2009-04-17 | 2010-05-05 | 广州阳普医疗科技股份有限公司 | Aluminum foil belt transmission mechanism for medical test tube |
| CN202264895U (en) * | 2011-09-06 | 2012-06-06 | 温州市倍可特医疗器械有限公司 | Plastic test tube sealing machine |
| CN202379699U (en) * | 2011-11-16 | 2012-08-15 | 广州阳普医疗科技股份有限公司 | Test tube opening film-sealing device |
| CN203439323U (en) * | 2013-08-26 | 2014-02-19 | 杨义良 | Full-automatic plastic uptake sealing packing machine |
| CN203474002U (en) * | 2013-09-12 | 2014-03-12 | 温州市沪华机械电器有限公司 | Membrane absorption device of filling sealing machine |
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| CN106479860A (en) | 2017-03-08 |
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