CN106497775A - A kind of quantitative real time PCR Instrument with automatic sealing function - Google Patents
A kind of quantitative real time PCR Instrument with automatic sealing function Download PDFInfo
- Publication number
- CN106497775A CN106497775A CN201610894588.9A CN201610894588A CN106497775A CN 106497775 A CN106497775 A CN 106497775A CN 201610894588 A CN201610894588 A CN 201610894588A CN 106497775 A CN106497775 A CN 106497775A
- Authority
- CN
- China
- Prior art keywords
- pcr instrument
- cylinder
- main frame
- motor
- electric magnet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000007789 sealing Methods 0.000 title claims abstract description 17
- 238000011529 RT qPCR Methods 0.000 title claims abstract description 13
- 210000004276 hyalin Anatomy 0.000 claims abstract description 21
- 239000012528 membrane Substances 0.000 claims abstract description 21
- 238000009434 installation Methods 0.000 claims description 15
- 230000008878 coupling Effects 0.000 claims description 3
- 238000010168 coupling process Methods 0.000 claims description 3
- 238000005859 coupling reaction Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 2
- 238000005096 rolling process Methods 0.000 claims 1
- 230000007306 turnover Effects 0.000 claims 1
- 238000004804 winding Methods 0.000 claims 1
- 241000973497 Siphonognathus argyrophanes Species 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Cultivation Of Plants (AREA)
Abstract
A kind of quantitative real time PCR Instrument with automatic sealing function,Belong to medical device technical field,Including the first cylinder、PCR instrument main frame、Cover、Electric pushrod、Blade、Bolt、Clamping plate、Hyaline membrane、First electric magnet、Guard circle、Second electric magnet、Second bearing pin、Second hollow out、Second support plate、Test tube rack、Support、Scissor arms、Second cylinder、Prop up block、First support plate、First hollow out、First bearing pin、Cylinder、Wheel carrier、Second motor、Controller、Pressing plate、Pole、First motor、Bearing and renovate,Install on the PCR instrument main frame and renovate,Mounting bracket in PCR instrument main frame,It is test tube rack to install on support,In PCR instrument main frame, the first support plate is installed on right side,First support plate is provided with the first hollow out,Two the first bearing pins are installed in the first hollow out,On the right side of scissor arms, upper and lower ends are respectively arranged on two the first bearing pins;It is an advantage of the invention that:There is automatic sealing function.
Description
Technical field
The present invention relates to a kind of quantitative real time PCR Instrument with automatic sealing function, belongs to medical device technical field.
Background technology
Real-time fluorescence quantitative PCR instrument, is made up of fluorescent quantitation system and computer, for monitoring the fluorescence of cyclic process.
Fluorescence data collected by the computer being connected with real-time device.Data are graphically shown by the real-time analysis software of exploitation
Show.Initial data is drawn into chart of the fluorescence intensity relative to period.Can start to analyze after source data collection.In real time
The software of equipment can make the data that collects carry out normalization process to make up the difference of background fluorescence.The fluorescence of prior art is fixed
Amount PCR instrument does not have the function of sealing automatically test tube, needs experimenter that test tube is put into fluorescent quantitation again after sealing manually
In PCR instrument, use pretty troublesome.In order to solve above-mentioned difficulties, need to develop a fluorescence with automatic sealing function calmly
Amount PCR instrument.
Content of the invention
It is an object of the invention to provide a kind of quantitative real time PCR Instrument with automatic sealing function.
The problem to be solved in the present invention is that the quantitative real time PCR Instrument of prior art does not have the function of sealing automatically test tube
Problem.
For realizing that the purpose of the present invention, the technical solution used in the present invention be:
A kind of quantitative real time PCR Instrument with automatic sealing function, including the first cylinder, PCR instrument main frame, cover, electric pushrod,
Blade, bolt, clamping plate, hyaline membrane, the first electric magnet, guard circle, the second electric magnet, the second bearing pin, the second hollow out, second
Plate, test tube rack, support, scissor arms, the second cylinder, block, the first support plate, the first hollow out, the first bearing pin, cylinder, wheel carrier, second
Motor, controller, pressing plate, pole, the first motor, bearing and renovate, install on the PCR instrument main frame and renovate, in PCR instrument main frame
Mounting bracket, it is that the first support plate is installed on right side in test tube rack, PCR instrument main frame to install on support, and the first support plate is provided with first and engraves
Sky, installs two the first bearing pins in the first hollow out, and on the right side of scissor arms, upper and lower ends are located at down respectively on two the first bearing pins
First bearing pin of side is connected with the piston rod of the second cylinder, and on a block, a block is arranged on PCR instrument main frame to the second cylinder
Interior, respectively on two the second bearing pins, two the second bearing pins are arranged in the second hollow out the upper and lower both sides of scissor arms left end, and second
Support plate is provided with the second hollow out, installs the second electric magnet on the left of the second support plate, and in PCR instrument main frame, left side wall installs guard circle, protects
First electric magnet is installed in guard ring, the first electric magnet is flushed with the second electric magnet, after the first electric magnet and the energization of the second electric magnet
Magnetic is attracting, installation bolt on clamping plate, and by Boards wall on the second support plate, in PCR instrument main frame, right side wall installs wheel carrier to bolt,
Mounting rotary drum on wheel carrier, the axle of cylinder are connected with the second motor by shaft coupling, and the second motor is twined on cylinder on wheel carrier
Around hyaline membrane, PCR instrument main frame inner top installs peace in two the first cylinders, installation cover on the piston rod of the first cylinder, cover
Dress bearing, the output shaft of the first motor are mounted on bearings, and the first motor is arranged on pressing plate, output shaft of the first motor or so
Pole is respectively installed in both sides, installs electric pushrod on pole, installs blade under electric pushrod, and controller is arranged on PCR instrument host computer side
On wall, controller by wire respectively with the first motor, the second motor, the first electric magnet, the second electric magnet and electric pushrod phase
Even.
Built-in first electromagnetic valve of first cylinder, the first electromagnetic valve are connected with controller by wire.
Built-in second electromagnetic valve of second cylinder, the second electromagnetic valve are connected with controller by wire.
The guard circle is quality of rubber materials.
It is an advantage of the invention that:Looser a bolt, controller controls the second electric motor starting, rotate cylinder, put hyaline membrane
Pine, hyaline membrane left end is moved under clamping plate, bolt is tightened, hyaline membrane is fixed under clamping plate;Controller controls the second cylinder and does
Stretching motion, extends scissor arms, is moved to the left the second electric magnet, makes the second electric magnet touch the first electric magnet;Control
After first electric magnet of device processed and the second electric magnet are powered, magnetic is attracting;Controller controls the first cylinder and does stretching motion, makes pressing plate
Move down, make pressing plate that hyaline membrane is pressed in above test tube mouth;Controller control electric pushrod does stretching motion, makes blade downward
Mobile;Controller controls the first electric motor starting, rotates pole, makes blade circumgyration incision hyaline membrane.
Description of the drawings
Fig. 1 is a kind of quantitative real time PCR Instrument overall structure figure with automatic sealing function of the present invention;
In figure:1st, the first cylinder 2, PCR instrument main frame 3, cover 4, electric pushrod 5, blade 6, bolt 7, clamping plate 8, transparent
Film 9, the first electric magnet 10, guard circle 11, the second electric magnet 12, the second bearing pin 13, the second hollow out 14, the second support plate
15th, test tube rack 16, support 17, scissor arms 18, the second cylinder 19, block 20, the first support plate 21, the first hollow out 22, the
One bearing pin 23, cylinder 24, wheel carrier 25, the second motor 26, controller 27, pressing plate 28, pole 29, the first motor 30,
Bearing 31, renovate.
Specific embodiment
Below in conjunction with the accompanying drawings and embodiment the present invention is further illustrated.
A kind of quantitative real time PCR Instrument with automatic sealing function of the present invention, including the first cylinder 1, PCR instrument main frame 2, cover
Son 3, electric pushrod 4, blade 5, bolt 6, clamping plate 7, hyaline membrane 8, the first electric magnet 9, guard circle 10, the second electric magnet 11, the
Two bearing pins 12, the second hollow out 13, the second support plate 14, test tube rack 15, support 16, scissor arms 17, the second cylinder 18, block 19, the
One support plate 20, the first hollow out 21, the first bearing pin 22, cylinder 23, wheel carrier 24, the second motor 25, controller 26, pressing plate 27, pole
28th, the first motor 29, bearing 30 and 31 are renovated, installs on the PCR instrument main frame 2 and renovate 31, mounting bracket in PCR instrument main frame 2
16, it is test tube rack 15 to install on support 16, and in PCR instrument main frame 2, the first support plate 20 is installed on right side, and the first support plate 20 is provided with first
Hollow out 21, installs two the first bearing pins 22 in the first hollow out 21,17 right side upper and lower ends of scissor arms are respectively sold installed in two first
On axle 22, underlying first bearing pin 22 is connected with the piston rod of the second cylinder 18, and the second cylinder 18 is arranged on a block 19,
Second cylinder, 18 built-in second electromagnetic valve, the second electromagnetic valve are connected with controller 26 by wire, and controller 26 controls the second gas
Cylinder 18 does stretching motion, extends scissor arms 17, is moved to the left the second electric magnet 11, makes the second electric magnet 11 touch
One electric magnet 9;Block 19 is propped up in PCR instrument main frame 2, the upper and lower both sides of 17 left end of scissor arms are respectively arranged on two the second bearing pins 12
On, in the second hollow out 13, the second support plate 14 is provided with the second hollow out 13 to two the second bearing pins 12, on the left of the second support plate 14
Second electric magnet 11 is installed, left side wall installs guard circle 10 in PCR instrument main frame 2, and guard circle 10 is quality of rubber materials, in guard circle 10
First electric magnet 9 is installed, the first electric magnet 9 is flushed with the second electric magnet 11, after the first electric magnet 9 and the energization of the second electric magnet 11
Magnetic is attracting, and after 26 first electric magnet 9 of controller and the energization of the second electric magnet 11, magnetic is attracting;Installation bolt 6, spiral shell on clamping plate 7
Bolt 6 is fixed on clamping plate 7 on second support plate 14, right side wall installation wheel carrier 24 in PCR instrument main frame 2, mounting rotary drum 23 on wheel carrier 24,
The axle of cylinder 23 is connected with the second motor 25 by shaft coupling, and the second motor 25 loosers a bolt 6 on wheel carrier 24, control
Device 26 controls the second motor 25 and starts, and rotates cylinder 23, loosens hyaline membrane 8,8 left end of hyaline membrane is moved under clamping plate 7, twists
Tight bolt 6, hyaline membrane 8 is fixed under clamping plate 7;Wind hyaline membrane 8 on cylinder 23,2 inner top of PCR instrument main frame installs two the
One cylinder 1,1 built-in first electromagnetic valve of the first cylinder, the first electromagnetic valve are connected with controller 26 by wire, and controller 26 is controlled
First cylinder 1 does stretching motion, moves down pressing plate 27, makes pressing plate 27 that hyaline membrane 8 is pressed in above test tube mouth;First cylinder
Installation cover 3 on 1 piston rod, installs bearing 30 in cover 3, the output shaft of the first motor 29 is arranged on bearing 30, and first
On pressing plate 27, the output shaft of the first motor 29 each side installs pole 28, the control of controller 26 first to motor 29
Motor 29 starts, and rotates pole 28, makes 5 circumgyration incision hyaline membrane 8 of blade, installs electric pushrod 4, electric pushrod on pole 28
4 times installation blades 5, the control electric pushrod 4 of controller 26 do stretching motion, move down blade 5;Controller 26 is arranged on
On 2 side wall of PCR instrument main frame, controller 26 by wire respectively with the first motor 29, the second motor 25, the first electric magnet 9, second
Electric magnet 11 is connected with electric pushrod 4.
Using method of the present invention:Looser a bolt 6, controller 26 controls the second motor 25 and starts, and rotates cylinder 23, makes
Bright film 8 loosens, and 8 left end of hyaline membrane is moved under clamping plate 7, bolt 6 is tightened, hyaline membrane 8 is fixed under clamping plate 7;Controller 26
Control the second cylinder 18 and do stretching motion, extend scissor arms 17, be moved to the left the second electric magnet 11, make the second electromagnetism
Ferrum 11 touch the first electric magnet 9;After 26 first electric magnet 9 of controller and the second electric magnet 11 are powered, magnetic is attracting;Controller 26
Control the first cylinder 1 and do stretching motion, move down pressing plate 27, make pressing plate 27 that hyaline membrane 8 is pressed in above test tube mouth;Control
The control electric pushrod 4 of device 26 does stretching motion, moves down blade 5;Controller 26 controls the first motor 29 and starts, and makes pole
28 rotations, make 5 circumgyration incision hyaline membrane 8 of blade.
Claims (4)
1. a kind of quantitative real time PCR Instrument with automatic sealing function, including the first cylinder(1), PCR instrument main frame(2), cover
(3), electric pushrod(4), blade(5), bolt(6), clamping plate(7), hyaline membrane(8), the first electric magnet(9), guard circle(10),
Two electric magnet(11), the second bearing pin(12), the second hollow out(13), the second support plate(14), test tube rack(15), support(16), scissor
Arm(17), the second cylinder(18), block(19), the first support plate(20), the first hollow out(21), the first bearing pin(22), cylinder(23)、
Wheel carrier(24), the second motor(25), controller(26), pressing plate(27), pole(28), the first motor(29), bearing(30)With turn over
Lid(31), it is characterized in that:The PCR instrument main frame(2)Upper installation is renovated(31), PCR instrument main frame(2)Interior mounting bracket(16),
Frame(16)Upper installation is test tube rack(15), PCR instrument main frame(2)Interior right side is installed by the first support plate(20), the first support plate(20)On set
There is the first hollow out(21), the first hollow out(21)Two the first bearing pins of upper installation(22), scissor arms(17)Right side upper and lower ends are respectively pacified
It is mounted in two the first bearing pins(22)On, underlying first bearing pin(22)With the second cylinder(18)Piston rod be connected, second
Cylinder(18)Installed in a block(19)On, a block(19)It is arranged on PCR instrument main frame(2)Interior, scissor arms(17)The upper and lower both sides of left end
Two second bearing pins are respectively arranged on(12)On, two the second bearing pins(12)It is arranged on the second hollow out(13)On, the second support plate(14)
It is provided with the second hollow out(13), the second support plate(14)Left side is installed by the second electric magnet(11), PCR instrument main frame(2)Interior left side wall peace
Dress guard circle(10), guard circle(10)The first electric magnet of interior installation(9), the first electric magnet(9)With the second electric magnet(11)Flush,
First electric magnet(9)With the second electric magnet(11)After energization, magnetic is attracting, clamping plate(7)Upper installation bolt(6), bolt(6)Will folder
Plate(7)It is fixed on the second support plate(14)On, PCR instrument main frame(2)Interior right side wall installs wheel carrier(24), wheel carrier(24)Upper mounting rotary drum
(23), cylinder(23)Axle by shaft coupling and the second motor(25)It is connected, the second motor(25)It is arranged on wheel carrier(24)On, rolling
Cylinder(23)Upper winding hyaline membrane(8), PCR instrument main frame(2)Inner top installs two the first cylinders(1), the first cylinder(1)Piston
Installation cover on bar(3), cover(3)Interior installation bearing(30), the first motor(29)Output shaft be arranged on bearing(30)On, the
One motor(29)It is arranged on pressing plate(27)On, the first motor(29)Output shaft pole is each side installed(28), pole
(28)Upper installation electric pushrod(4), electric pushrod(4)Lower installation blade(5), controller(26)It is arranged on PCR instrument main frame(2)Side
On wall, controller(26)By wire respectively with the first motor(29), the second motor(25), the first electric magnet(9), the second electromagnetism
Ferrum(11)And electric pushrod(4)It is connected.
2. a kind of quantitative real time PCR Instrument with automatic sealing function according to claim 1, is characterized in that:Described
One cylinder(1)Built-in first electromagnetic valve, the first electromagnetic valve is by wire and controller(26)It is connected.
3. a kind of quantitative real time PCR Instrument with automatic sealing function according to claim 1, is characterized in that:Described
Two cylinders(18)Built-in second electromagnetic valve, the second electromagnetic valve is by wire and controller(26)It is connected.
4. a kind of quantitative real time PCR Instrument with automatic sealing function according to claim 1, is characterized in that:The guarantor
Guard ring(10)For quality of rubber materials.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610894588.9A CN106497775B (en) | 2016-10-14 | 2016-10-14 | A kind of fluorescence quantitative PCR instrument with automatic sealing function |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610894588.9A CN106497775B (en) | 2016-10-14 | 2016-10-14 | A kind of fluorescence quantitative PCR instrument with automatic sealing function |
Publications (2)
Publication Number | Publication Date |
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CN106497775A true CN106497775A (en) | 2017-03-15 |
CN106497775B CN106497775B (en) | 2019-03-15 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201610894588.9A Expired - Fee Related CN106497775B (en) | 2016-10-14 | 2016-10-14 | A kind of fluorescence quantitative PCR instrument with automatic sealing function |
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CN (1) | CN106497775B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114988185A (en) * | 2022-08-08 | 2022-09-02 | 启东新朋莱纺织科技有限公司 | Guide device is used in textile fabric processing that facilitates use |
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US20090301661A1 (en) * | 2006-05-12 | 2009-12-10 | Roman Kammler | Device for welding a film web |
CN202264895U (en) * | 2011-09-06 | 2012-06-06 | 温州市倍可特医疗器械有限公司 | Plastic test tube sealing machine |
CN203528909U (en) * | 2013-09-03 | 2014-04-09 | 天津金勇纸制品有限公司 | Aluminum foil film sealing machine |
CN105151426A (en) * | 2015-09-01 | 2015-12-16 | 天津名唐环保科技有限公司 | Film sealing machine with warning function |
CN105936869A (en) * | 2016-06-23 | 2016-09-14 | 浙江爱易生物医学科技有限公司 | Multifunctional fluorescent quantitative PCR instrument |
CN205589587U (en) * | 2015-12-22 | 2016-09-21 | 天津中益包装设备有限公司 | Quick back of body of small bag package seals packagine machine |
CN105969638A (en) * | 2016-06-23 | 2016-09-28 | 浙江爱易生物医学科技有限公司 | Adjustable fluorogenic quantitative PCR instrument |
-
2016
- 2016-10-14 CN CN201610894588.9A patent/CN106497775B/en not_active Expired - Fee Related
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090301661A1 (en) * | 2006-05-12 | 2009-12-10 | Roman Kammler | Device for welding a film web |
CN202264895U (en) * | 2011-09-06 | 2012-06-06 | 温州市倍可特医疗器械有限公司 | Plastic test tube sealing machine |
CN203528909U (en) * | 2013-09-03 | 2014-04-09 | 天津金勇纸制品有限公司 | Aluminum foil film sealing machine |
CN105151426A (en) * | 2015-09-01 | 2015-12-16 | 天津名唐环保科技有限公司 | Film sealing machine with warning function |
CN205589587U (en) * | 2015-12-22 | 2016-09-21 | 天津中益包装设备有限公司 | Quick back of body of small bag package seals packagine machine |
CN105936869A (en) * | 2016-06-23 | 2016-09-14 | 浙江爱易生物医学科技有限公司 | Multifunctional fluorescent quantitative PCR instrument |
CN105969638A (en) * | 2016-06-23 | 2016-09-28 | 浙江爱易生物医学科技有限公司 | Adjustable fluorogenic quantitative PCR instrument |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114988185A (en) * | 2022-08-08 | 2022-09-02 | 启东新朋莱纺织科技有限公司 | Guide device is used in textile fabric processing that facilitates use |
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Granted publication date: 20190315 |