CN105969638A - Adjustable fluorogenic quantitative PCR instrument - Google Patents
Adjustable fluorogenic quantitative PCR instrument Download PDFInfo
- Publication number
- CN105969638A CN105969638A CN201610460165.6A CN201610460165A CN105969638A CN 105969638 A CN105969638 A CN 105969638A CN 201610460165 A CN201610460165 A CN 201610460165A CN 105969638 A CN105969638 A CN 105969638A
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- China
- Prior art keywords
- electric magnet
- pcr instrument
- electric
- cylinder
- support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000003753 real-time PCR Methods 0.000 title abstract 3
- 239000004973 liquid crystal related substance Substances 0.000 claims abstract description 31
- 238000011529 RT qPCR Methods 0.000 claims description 11
- 238000009434 installation Methods 0.000 claims description 2
- 230000005611 electricity Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Liquid Crystal (AREA)
Abstract
An adjustable fluorogenic quantitative PCR instrument comprises a sample plate, a strut, a rack, a gear motor, a first electric drive pusher, a first electromagnet, a second electromagnet, a first backing plate, support blocks, a liquid crystal display screen, a spring, second backing plates, a fourth electromagnet, a third electromagnet, a second electric drive pusher, a moving block, a linear motor, a PCR instrument mainframe, a controller, lower clamping plates, upper clamping plates, sliding blocks, sliding rails, support rods, a support, a groove, second air cylinders, first air cylinders and a flap, wherein the strut is mounted in the PCR instrument mainframe, the sample plate is placed on the strut, the flap is hinged and mounted on the PCR instrument mainframe, the groove is formed in the flap, the first air cylinders are mounted, the piston rods of the first air cylinders extend out of the groove, the support is mounted on the piston rods of the first air cylinders, the support rods are mounted on the left side and the right side below the support, the second air cylinders are mounted at front ends of the support rods, and the upper clamping plates are mounted below the second air cylinders. The adjustable fluorogenic quantitative PCR instrument has the advantage that the orientation of the liquid crystal display screen can be adjusted.
Description
Technical field
The present invention relates to a kind of scalable quantitative real time PCR Instrument, belong to gene detection equipment technical field.
Background technology
Quantitative real time PCR Instrument is specific target gene detection and quantitative unified platform, and by PCR thermal cycle, fluorescence is examined
Survey and various analysis softwares combine, can dynamically observe in the PCR each reaction tube of each circulation pcr amplification product by
Cumulative situation about adding.The liquid crystal display of the PCR instrument of prior art is all fixedly mounted on PCR instrument main frame, it is impossible to regulation is adjusted
Joint liquid crystal display towards, make reading inconvenient.In order to solve above-mentioned difficulties, need to develop a energy adjustable liquid crystal display display
Towards scalable quantitative real time PCR Instrument.
Summary of the invention
It is an object of the invention to provide a kind of scalable quantitative real time PCR Instrument.
The problem to be solved in the present invention is that the liquid crystal display of the PCR instrument of prior art is all fixedly mounted at PCR instrument master
Problem on machine.
For realizing the purpose of the present invention, the technical solution used in the present invention is:
A kind of scalable quantitative real time PCR Instrument, including sample panel, support, frame, reducing motor, the first electric pushrod, the first electricity
Magnet, the second electric magnet, the first backing plate, block, liquid crystal display screen, spring, the second backing plate, the 4th electric magnet, the 3rd electric magnet, second
Electric pushrod, movable block, linear electric motors, PCR instrument main frame, controller, lower plate, train wheel bridge, slide block, slide rail, pole, support,
Groove, the second cylinder, the first cylinder and renovate, install support in described PCR instrument main frame, support is placed sample panel, PCR instrument master
On machine, hinged installation is renovated, and is provided with groove in renovating, and installs the first cylinder, and the piston rod of the first cylinder stretches to outside groove, and first
Mounting bracket on the piston rod of cylinder, each side installs pole under support, the second cylinder, the second cylinder are installed in pole front end
Under install clamping plate, train wheel bridge is arranged on slide block, and slide block is arranged on slide rail, and slide rail is arranged under pole, slide rail lower end pacify
Load clamping plate, linear electric motors are installed on the left of PCR instrument main frame, linear electric motors are installed movable block, spring, bullet are installed on the left of movable block
Liquid crystal display screen is installed on spring, liquid crystal display screen is installed the first backing plate, the first backing plate is installed the second electric magnet, sets above the second electric magnet
Having the first electric magnet, after the first electric magnet and the energising of the second electric magnet, magnetic is attracting, and the first electric magnet is arranged on first and electronic pushes away
Bar lower end, the first electric pushrod is arranged on the output shaft of reducing motor, and reducing motor is arranged in frame, and frame is arranged on directly
On line motor, a block is respectively installed in the upper and lower both sides of movable block, and a block is installed the second electric pushrod, and the second electric pushrod front end is installed
3rd electric magnet, on the right side of liquid crystal display screen, the second backing plate is respectively installed at upper and lower two ends, and the second backing plate is installed the 4th electromagnetic valve, the 4th electromagnetism
Ferrum and the 3rd electric magnet are positioned at same level, and after the 3rd electric magnet and the energising of the 4th electric magnet, magnetic is attracting, and controller is installed
On PCR instrument main frame, controller by wire respectively with reducing motor, linear electric motors, the first electric pushrod, second electronic push away
Bar, the first electric magnet, the second electric magnet, the 3rd electric magnet and the 4th electric magnet are connected.
Built-in first electromagnetic valve of described first cylinder, the first electromagnetic valve is connected with controller by wire.
Built-in second electromagnetic valve of described second cylinder, the second electromagnetic valve is connected with controller by wire.
The invention have the advantage that and sealed paper each side put between lower plate and train wheel bridge, controller controls
Second cylinder does stretching motion, makes train wheel bridge move down, and makes train wheel bridge sealed paper be fixed on lower plate；Cover and renovate,
Controller controls the first cylinder and does stretching motion, makes support move down, and makes sealed paper be moved down into above sample panel, makes close
Envelope paper covering tightly is above sample panel；Controller controls linear electric motors and starts, and makes movable block move up and down；Controller controls
First electric pushrod starts, and makes liquid crystal display screen move up and down；Controller controls magnetic after the first electric magnet and the energising of the second electric magnet
Attracting, make the 3rd electric magnet and the 4th electric magnet no power；Controller controls reducing motor and starts, and makes liquid crystal display screen rotate, adjusts
Joint liquid crystal display screen towards；Controller controls the first electric magnet and the second electric magnet power-off, makes the 3rd electric magnet and the 4th electric magnet
After energising, magnetic is attracting；Controller controls the second electric pushrod and does stretching motion, makes liquid crystal display screen towards lower swing or swings upward, side
Just data are read.
Accompanying drawing explanation
Fig. 1 is one scalable quantitative real time PCR Instrument overall structure figure of the present invention；
In figure: 1, sample panel 2, support 3, frame 4, reducing motor the 5, first electric pushrod the 6, first electric magnet 7, second
Electric magnet the 8, first backing plate 9, block 10, liquid crystal display screen 11, spring the 12, second backing plate the 13, the 4th electric magnet the 14, the 3rd
Electric magnet the 15, second electric pushrod 16, movable block 17, linear electric motors 18, PCR instrument main frame 19, controller 20, lower plate
21, train wheel bridge 22, slide block 23, slide rail 24, pole 25, support 26, groove the 27, second cylinder the 28, first cylinder 29,
Renovate.
Detailed description of the invention
Below in conjunction with the accompanying drawings and embodiment the present invention is further illustrated.
One scalable quantitative real time PCR Instrument of the present invention, including sample panel 1, support 2, frame 3, reducing motor 4, first
Electric pushrod the 5, first electric magnet the 6, second electric magnet the 7, first backing plate 8, block 9, liquid crystal display screen 10, spring the 11, second backing plate 12,
4th electric magnet the 13, the 3rd electric magnet the 14, second electric pushrod 15, movable block 16, linear electric motors 17, PCR instrument main frame 18, control
Device 19, lower plate 20, train wheel bridge 21, slide block 22, slide rail 23, pole 24, support 25, groove the 26, second cylinder the 27, first cylinder
28 and renovate 29, in described PCR instrument main frame 18, support 2 is installed, support 2 is placed sample panel 1, hinged peace on PCR instrument main frame 18
Dress renovates 29, is provided with groove 26, installs the first cylinder 28, the first built-in first electromagnetic valve of cylinder 28, the first electromagnetism in renovating 29
Valve is connected with controller 19 by wire, covers and renovates 29, and controller 19 controls the first cylinder 28 and does stretching motion, makes support 25
Move down, make sealed paper be moved down into above sample panel 1, make sealed paper covering tightly above sample panel 1；First gas
The piston rod of cylinder 28 stretches to outside groove 26, mounting bracket 25 on the piston rod of the first cylinder 28, and support is each side pacified for 25 times
Filling pole 24, the second cylinder 27 is installed in pole 24 front end, and the second built-in second electromagnetic valve of cylinder 27, the second electromagnetic valve passes through wire
Being connected with controller 19, sealed paper each side put between lower plate 20 and train wheel bridge 21, controller 19 controls
Two cylinders 27 do stretching motion, make train wheel bridge 21 move down, and make train wheel bridge 21 sealed paper be fixed on lower plate 20；Second
Cylinder installs clamping plate 21 for 27 times, and train wheel bridge 21 is arranged on slide block 22, and slide block 22 is arranged on slide rail 23, and slide rail 23 is arranged on
Pole 24 times, slide rail 23 lower end is installed lower plate 20, is installed linear electric motors 17, linear electric motors 17 are pacified on the left of PCR instrument main frame 18
Dress movable block 16, controller 19 controls linear electric motors 17 and starts, and makes movable block 16 move up and down；Control that Qing Kou town first is electronic pushes away
Bar 5 starts, and makes liquid crystal display screen 10 move up and down；Spring 11 is installed on the left of movable block 16, spring 11 is installed liquid crystal display screen 10, liquid crystal display screen
Installing the first backing plate 8 on 10, the first backing plate 8 is installed the second electric magnet 7, the second electric magnet 7 is arranged over the first electric magnet 6,
After first electric magnet 6 and the energising of the second electric magnet 7, magnetic is attracting, and controller 19 controls the first electric magnet 6 and the second electric magnet 7 leads to
After electricity, magnetic is attracting, makes the 3rd electric magnet 14 and the 4th electric magnet 13 no power；First electric magnet 6 is arranged on the first electric pushrod
5 lower ends, the first electric pushrod 5 is arranged on the output shaft of reducing motor 4, and reducing motor 4 is arranged in frame 3, controller 19
Control reducing motor 4 start, make liquid crystal display screen 10 rotate, come adjustable liquid crystal display screen 10 towards；Frame 3 is arranged on linear electric motors 17
On, a block 9 is respectively installed in movable block about 16 both sides, and a block 9 is installed the second electric pushrod 15, the second electric pushrod 15 front end peace
Filling the 3rd electric magnet 14, on the right side of liquid crystal display screen 10, the second backing plate 12 is respectively installed at upper and lower two ends, and the second backing plate 12 is installed the 4th electromagnetism
Valve, the 4th electric magnet 13 and the 3rd electric magnet 14 are positioned at same level, after the 3rd electric magnet 14 and the energising of the 4th electric magnet 13
Magnetic is attracting, and controller 19 controls the first electric magnet 6 and the second electric magnet 7 power-off, makes the 3rd electric magnet 14 and the 4th electric magnet
After 13 energisings, magnetic is attracting；Controller 19 controls the second electric pushrod 15 and does stretching motion, makes liquid crystal display screen 10 towards lower swing or court
Upper swing, convenient reading data, controller 19 is arranged on PCR instrument main frame 18, and controller 19 is electric with deceleration respectively by wire
Machine 4, linear electric motors the 17, first electric pushrod the 5, second electric pushrod the 15, first electric magnet the 6, second electric magnet the 7, the 3rd electromagnetism
Ferrum 14 is connected with the 4th electric magnet 13.
Using method of the present invention: sealed paper is each side put between lower plate 20 and train wheel bridge 21, controller
19 control the second cylinder 27 does stretching motion, makes train wheel bridge 21 move down, makes train wheel bridge 21 that sealed paper is fixed on lower plate
On 20；Covering and renovate 29, controller 19 controls the first cylinder 28 and does stretching motion, makes support 25 move down, make sealed paper to
It is moved to above sample panel 1, makes sealed paper covering tightly above sample panel 1；Controller 19 controls linear electric motors 17 and opens
Dynamic, make movable block 16 move up and down；Controller controls the first electric pushrod 5 and starts, and makes liquid crystal display screen 10 move up and down；Controller 19
After controlling the first electric magnet 6 and the energising of the second electric magnet 7, magnetic is attracting, makes the 3rd electric magnet 14 and the 4th electric magnet 13 obstructed
Electricity；Controller 19 controls reducing motor 4 and starts, and makes liquid crystal display screen 10 rotate, come adjustable liquid crystal display screen 10 towards；Controller 19 controls
First electric magnet 6 and the second electric magnet 7 power-off, after making the 3rd electric magnet 14 and the energising of the 4th electric magnet 13, magnetic is attracting；Control
Device 19 controls the second electric pushrod 15 and does stretching motion, makes liquid crystal display screen 10 towards lower swing or swings upward, convenient reading data.
Claims (3)
1. a scalable quantitative real time PCR Instrument, including sample panel (1), support (2), frame (3), reducing motor (4), first
Electric pushrod (5), the first electric magnet (6), the second electric magnet (7), the first backing plate (8), block (9), liquid crystal display screen (10), spring
(11), the second backing plate (12), the 4th electric magnet (13), the 3rd electric magnet (14), the second electric pushrod (15), movable block (16),
Linear electric motors (17), PCR instrument main frame (18), controller (19), lower plate (20), train wheel bridge (21), slide block (22), slide rail
(23), pole (24), support (25), groove (26), the second cylinder (27), the first cylinder (28) and renovate (29), it is characterized in that:
Support (2) is installed in described PCR instrument main frame (18), support (2) is placed sample panel (1), the upper hinged installation of PCR instrument main frame (18)
Renovating (29), be provided with groove (26) in renovating (29), install the first cylinder (28), the piston rod of the first cylinder (28) stretches to groove
(26) outward, mounting bracket (25) on the piston rod of the first cylinder (28), pole (24) is each side installed under support (25),
The second cylinder (27) is installed in bar (24) front end, installs clamping plate (21) under the second cylinder (27), and train wheel bridge (21) is arranged on slide block
(22) on, slide block (22) is arranged on slide rail (23), and slide rail (23) is arranged under pole (24), and lower folder is installed in slide rail (23) lower end
Plate (20), PCR instrument main frame (18) left side is installed linear electric motors (17), linear electric motors (17) is installed movable block (16), movable block
(16) spring (11) is installed in left side, and spring (11) is installed liquid crystal display screen (10), and liquid crystal display screen (10) is upper installs the first backing plate (8), the
One backing plate (8) is upper installs the second electric magnet (7), and the second electric magnet (7) is arranged over the first electric magnet (6), the first electric magnet (6)
After being energized with the second electric magnet (7), magnetic is attracting, and the first electric magnet (6) is arranged on the first electric pushrod (5) lower end, and first is electronic
Push rod (5) is arranged on the output shaft of reducing motor (4), and reducing motor (4) is arranged in frame (3), and frame (3) is arranged on directly
On line motor (17), a block (9) is respectively installed in movable block (16) both sides up and down, and a block (9) is upper installs the second electric pushrod (15), the
The 3rd electric magnet (14) is installed in two electric pushrods (15) front end, and the second backing plate is respectively installed in two ends up and down in liquid crystal display screen (10) right side
(12), upper the 4th electromagnetic valve of installing of the second backing plate (12), the 4th electric magnet (13) and the 3rd electric magnet (14) are positioned at same level
After face, the 3rd electric magnet (14) and the energising of the 4th electric magnet (13), magnetic is attracting, and controller (19) is arranged on PCR instrument main frame (18)
On, controller (19) by wire respectively with reducing motor (4), linear electric motors (17), the first electric pushrod (5), second electronic
Push rod (15), the first electric magnet (6), the second electric magnet (7), the 3rd electric magnet (14) are connected with the 4th electric magnet (13).
A kind of scalable quantitative real time PCR Instrument the most according to claim 1, is characterized in that: in described first cylinder (28)
Putting the first electromagnetic valve, the first electromagnetic valve is connected with controller (19) by wire.
A kind of scalable quantitative real time PCR Instrument the most according to claim 1, is characterized in that: in described second cylinder (27)
Putting the second electromagnetic valve, the second electromagnetic valve is connected with controller (19) by wire.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610460165.6A CN105969638B (en) | 2016-06-23 | 2016-06-23 | A kind of adjustable fluorescence quantitative PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610460165.6A CN105969638B (en) | 2016-06-23 | 2016-06-23 | A kind of adjustable fluorescence quantitative PCR instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105969638A true CN105969638A (en) | 2016-09-28 |
| CN105969638B CN105969638B (en) | 2018-11-30 |
Family
ID=57022530
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610460165.6A Expired - Fee Related CN105969638B (en) | 2016-06-23 | 2016-06-23 | A kind of adjustable fluorescence quantitative PCR instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105969638B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244446A (en) * | 2016-10-14 | 2016-12-21 | 上海爱易生物医学科技股份有限公司 | A kind of scalable quantitative real time PCR Instrument |
| CN106318863A (en) * | 2016-10-14 | 2017-01-11 | 上海爱易生物医学科技股份有限公司 | System for gene detection |
| CN106497775A (en) * | 2016-10-14 | 2017-03-15 | 上海爱易生物医学科技股份有限公司 | A kind of quantitative real time PCR Instrument with automatic sealing function |
| CN106497776A (en) * | 2016-10-14 | 2017-03-15 | 上海爱易生物医学科技股份有限公司 | A kind of quantitative real time PCR Instrument with automatic regulation function |
| CN110283694A (en) * | 2019-07-02 | 2019-09-27 | 江苏农牧科技职业学院 | A kind of adjustable fluorescence quantitative PCR instrument |
| CN114196528A (en) * | 2022-01-18 | 2022-03-18 | 奥谱天成（厦门）光电有限公司 | PCR detector based on it is multispectral |
| JP2022058157A (en) * | 2020-09-30 | 2022-04-11 | 富佳生技股▲ふん▼有限公司 | Nucleic acid detection host, and nucleic acid detection device |
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| CN202279818U (en) * | 2011-11-09 | 2012-06-20 | 黑龙江八一农垦大学 | Support bracket ensuring visible liquid level and placing stability of PCR (polymerase chain reaction) tube |
| CN104673667A (en) * | 2015-03-03 | 2015-06-03 | 杭州博日科技有限公司 | Thermal block of PCR (polymerase chain reaction) fluorescent quantitative analyzer |
| CN105188938A (en) * | 2013-03-14 | 2015-12-23 | 简·探针公司 | Diagnostic systems and methods |
| CN105400680A (en) * | 2015-12-11 | 2016-03-16 | 舟山医院 | A novel fluorescent quantitative PCR instrument |
| CN105400691A (en) * | 2015-12-11 | 2016-03-16 | 舟山医院 | A multifunctional PCR instrument |
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2016
- 2016-06-23 CN CN201610460165.6A patent/CN105969638B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202279818U (en) * | 2011-11-09 | 2012-06-20 | 黑龙江八一农垦大学 | Support bracket ensuring visible liquid level and placing stability of PCR (polymerase chain reaction) tube |
| CN105188938A (en) * | 2013-03-14 | 2015-12-23 | 简·探针公司 | Diagnostic systems and methods |
| CN104673667A (en) * | 2015-03-03 | 2015-06-03 | 杭州博日科技有限公司 | Thermal block of PCR (polymerase chain reaction) fluorescent quantitative analyzer |
| CN105400680A (en) * | 2015-12-11 | 2016-03-16 | 舟山医院 | A novel fluorescent quantitative PCR instrument |
| CN105400691A (en) * | 2015-12-11 | 2016-03-16 | 舟山医院 | A multifunctional PCR instrument |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244446A (en) * | 2016-10-14 | 2016-12-21 | 上海爱易生物医学科技股份有限公司 | A kind of scalable quantitative real time PCR Instrument |
| CN106318863A (en) * | 2016-10-14 | 2017-01-11 | 上海爱易生物医学科技股份有限公司 | System for gene detection |
| CN106497775A (en) * | 2016-10-14 | 2017-03-15 | 上海爱易生物医学科技股份有限公司 | A kind of quantitative real time PCR Instrument with automatic sealing function |
| CN106497776A (en) * | 2016-10-14 | 2017-03-15 | 上海爱易生物医学科技股份有限公司 | A kind of quantitative real time PCR Instrument with automatic regulation function |
| CN106244446B (en) * | 2016-10-14 | 2018-10-09 | 上海爱易生物医学科技股份有限公司 | A kind of adjustable fluorescence quantitative PCR instrument |
| CN106497775B (en) * | 2016-10-14 | 2019-03-15 | 上海爱易生物医学科技股份有限公司 | A kind of fluorescence quantitative PCR instrument with automatic sealing function |
| CN110283694A (en) * | 2019-07-02 | 2019-09-27 | 江苏农牧科技职业学院 | A kind of adjustable fluorescence quantitative PCR instrument |
| JP2022058157A (en) * | 2020-09-30 | 2022-04-11 | 富佳生技股▲ふん▼有限公司 | Nucleic acid detection host, and nucleic acid detection device |
| CN114196528A (en) * | 2022-01-18 | 2022-03-18 | 奥谱天成（厦门）光电有限公司 | PCR detector based on it is multispectral |
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| Publication number | Publication date |
|---|---|
| CN105969638B (en) | 2018-11-30 |
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