CN104673667A - Thermal block of PCR (polymerase chain reaction) fluorescent quantitative analyzer - Google Patents
Thermal block of PCR (polymerase chain reaction) fluorescent quantitative analyzer Download PDFInfo
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- CN104673667A CN104673667A CN201510094330.6A CN201510094330A CN104673667A CN 104673667 A CN104673667 A CN 104673667A CN 201510094330 A CN201510094330 A CN 201510094330A CN 104673667 A CN104673667 A CN 104673667A
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- install bin
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Abstract
The invention discloses a thermal block of a PCR (polymerase chain reaction) fluorescent quantitative analyzer, and aims to solve the problems that a conventional PCR thermal block pressure mechanism is not applicable to test tubes with different heights, a full-automatic thermal block is driven by two power sources and the cost is high. One slider on each of two linear guide rails is fixed with a driving plate, and the other slider is fixed with a thermal block bracket; power is transmitted to a mounting box through the driving plate, push rods and connecting rods; two ends of the mounting box as well as a left limit groove and a right limit groove form rolling pairs; limit bolts penetrate through the mounting box and a pressure adjusting plate and are in threaded connection with an aluminum plate, and one limit bolt is sleeved with springs; a thread sleeve is fixed with the pressure adjusting plate, the bottom end of a screw is connected with the thread sleeve through a thread pair, and the top end is embedded in a square groove of a screw connecting shaft; the screw connecting shaft is hinged with the thermal block bracket, and a manual gear is fixed with the screw connecting shaft; a manual rack is fixed on the driving plate and meshed with the manual gear. The thermal block can be applicable to the test tubes with different heights, and motion of the thermal block in two directions is controlled by one power source.
Description
Technical field
The invention belongs to field of mechanical technique, be specifically related to a kind of PCR quantitative fluorescence analysis instrument heat lid.
Background technology
PCR instrument has another name called gene-amplificative instrament, Thermal Cycler, is actually the temperature cycler of a kind of PLC technology, quick changeable temperature and precise temperature control, is the indispensable indispensable instrument of life science and teaching field.Current PCR hot lid pressure mechanism is mainly applicable to highly suitable test tube, and can not be applicable to different size height test tube simultaneously.Full automatic heat lid is driven by two different propulsion sources, this considerably increases production cost.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of PCR quantitative fluorescence analysis instrument heat lid is provided, the test tube of different heights can be adapted to, and control the motion on two directions by a propulsion source, improve hot lid serviceability, reduce production cost.
The present invention includes installation base plate, left limit groove, right limit groove, motor, transmitting gear, follower gear, push rod, union lever, pedestal fairload, install bin, aluminium sheet, spring, pressure regulator panel, caging bolt, screw rod, swivel nut, hot lid support, manual gear, Hand-shifting wheel, screw rod coupling shaft, driver plate, locating stop piece, driven tooth bar, manual tooth bar, line slide rail and strongback.Described left limit groove, right limit groove, motor mounting rack and strongback are all fixed by screws on installation base plate, the fixed block of two line slide rails is fixed on installation base plate by screw symmetry, and two pieces of sliding shoes of each line slide rail are all fixed with slide block by screw and install rebound; Described driver plate is installed rebound by the symmetrically arranged two pieces of slide blocks of two blocks of side plates and two ends and is fixed, and hot lid support is installed rebound by two pieces of bracing frames and other two pieces of slide blocks and fixed; One end of described locating stop piece and one block of side plate are fixed by screw, and the other end is arranged on above strongback; Described motor is fixed on motor mounting rack; Transmitting gear is fixed on the output shaft of motor, and follower gear is hinged on motor mounting rack, and driven tooth bar is fixed on driver plate, and follower gear engages with transmitting gear and driven tooth bar simultaneously; Two push rods are symmetrical arranged, and one end is all fixed by screws on driver plate, and the other end is hinged with one end of a union lever respectively; Side walls hinged all with install bin of the other end of two union levers; The two ends symmetry of described install bin is hinged with two pedestal fairloads, and two pedestal fairloads form rolling pair with left limit groove, right limit groove respectively; Aluminium sheet and pressure regulator panel are all arranged in install bin, and heating film, heat insulating mattress and insulation plate are successively set on aluminium sheet, and are fixed by multiple securing bolt of equidistant arrangement and aluminium sheet; Be equipped with spring between all securing bolts and pressure regulator panel, pressure regulator panel compresses the inwall of install bin; Each spring is nested with on a caging bolt, and all caging bolts all through install bin, pressure regulator panel and insulation plate, and are threaded with aluminium sheet; Described swivel nut and pressure regulator panel are fixed, and one end that bottom and the swivel nut of screw rod stretch out outside install bin is connected by screw thread pair; Pressing plate is fixed by screws in outside install bin, and the shaft shoulder of clamping screw; The top of screw rod embeds in the square groove bottom screw rod coupling shaft; Described screw rod coupling shaft and hot lid support form revolute pair, and manual gear and Hand-shifting wheel are all fixed with screw rod coupling shaft; Described manual tooth bar is fixed on driver plate, and engages with manual gear.
Described left limit groove and the slideway of right limit groove all L-shaped, comprise vertical slideway and the horizontal slide at top of bottom.
Sealing-ring is fixed with bottom described install bin.
Described pressure regulator panel is fixed with symmetrically arranged two the first spacing guide pillars and two the second spacing guide pillars, the top of two the second spacing guide pillars embeds in the stopper slot of the correspondence limited block be fixed on hot lid support respectively.
Described screw rod coupling shaft is fixed with back-up ring, and Hand-shifting wheel is nested with on screw rod coupling shaft; Back-up ring pressing plate compresses back-up ring, and is fixed by screw with Hand-shifting wheel.
The beneficial effect had of the present invention:
The present invention realizes the different motion in two directions by a propulsion source, and can apply fixation pressure to test tube; After heat lid compresses test tube, motor no longer produces locking force to union lever, thus greatly improves the work-ing life of motor.The present invention can give heat lid different pressure according to the test tube of different heights; Can manual unlocking heat lid under powering-off state.
Accompanying drawing explanation
Fig. 1 is one-piece construction stereographic map of the present invention;
Fig. 2 is vertical view of the present invention;
Fig. 3 is the A-A sectional view of Fig. 2;
Fig. 4 is the side-view of Fig. 2;
Fig. 5 is the assembling schematic diagram of manual gear in the present invention, Hand-shifting wheel, screw rod coupling shaft, back-up ring and back-up ring pressing plate.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described.
As Fig. 1, 2, 3, shown in 4 and 5, a kind of PCR quantitative fluorescence analysis instrument heat lid, comprise installation base plate 1, left limit groove 2, right limit groove 3, motor 5, transmitting gear 6, follower gear 7, push rod 8, union lever 9, pedestal fairload 11, install bin 12, aluminium sheet 15, spring 19, pressure regulator panel 20, caging bolt 21, screw rod 24, swivel nut 25, hot lid support 26, manual gear 29, Hand-shifting wheel 30, screw rod coupling shaft 31, back-up ring 32, back-up ring pressing plate 33, driver plate 35, locating stop piece 37, driven tooth bar 38, manual tooth bar 39, line slide rail 40 and strongback 41.Left limit groove 2, right limit groove 3, motor mounting rack 4 and strongback 41 are all fixed by screws on installation base plate 1, the fixed block of two line slide rails 40 is fixed on installation base plate 1 by screw symmetry, and two pieces of sliding shoes of each line slide rail 40 are all fixed with slide block by screw and install rebound 28; Driver plate 35 is installed rebound 28 by two blocks of side plates 36 and the symmetrically arranged two pieces of slide blocks in two ends and is fixed, and hot lid support 26 is installed rebound 28 by two pieces of bracing frames 27 and other two pieces of slide blocks and fixed; One end of locating stop piece 37 and one block of side plate 36 are fixed by screw, and the other end is arranged on above strongback 41; Motor 5 is fixed by screws on motor mounting rack 4; Transmitting gear 6 is fixed by screws on the output shaft of motor 5, and follower gear 7 is hinged on motor mounting rack 4, and driven tooth bar 38 is fixed by screws on driver plate 35, and follower gear 7 engages with transmitting gear 6 and driven tooth bar 38 simultaneously; Two push rods 8 are symmetrical arranged, and one end is all fixed by screws on driver plate 35, and the other end is hinged by pin 10 with one end of a union lever 9 respectively; Side walls hinged all with install bin 12 of the other end of two union levers 9; The two ends symmetry of install bin 12 is hinged with two pedestal fairloads, 11, two pedestal fairloads 11 and forms rolling pair with left limit groove 2, right limit groove 3 respectively; The slideway of left limit groove 2 and right limit groove 3 is all L-shaped, comprises the vertical slideway of bottom and the horizontal slide at top; Sealing-ring 34 is fixed on bottom install bin 12, and aluminium sheet 15 and pressure regulator panel 20 are all arranged in install bin 12, and heating film 16, heat insulating mattress 17 and insulation plate 13 are successively set on aluminium sheet 15, and is fixed with aluminium sheet 15 by four securing bolts 18 of equidistant arrangement; Be equipped with spring 19 between four securing bolts 18 and pressure regulator panel 20, pressure regulator panel 20 compresses the inwall of install bin 12; Four springs 19 are nested with respectively on a caging bolt 21, and four caging bolts 21 all through install bin 12, pressure regulator panel 20 and insulation plate 13, and are threaded with aluminium sheet 15; Pressure regulator panel 20 is fixed with symmetrically arranged two the first spacing guide pillars 22 and two the second spacing guide pillars 23, the top of two the second spacing guide pillars 23 embeds in the stopper slot of the correspondence limited block be fixed on hot lid support 26 respectively, the rotation of restriction install bin 12.Swivel nut 25 is fixed with pressure regulator panel 20, and one end that bottom and the swivel nut 25 of screw rod 24 stretch out outside install bin 12 is connected by screw thread pair; Pressing plate 14 is fixed by screws in outside install bin 12, and the shaft shoulder of clamping screw 24; The top of screw rod 24 embeds in the square groove bottom screw rod coupling shaft 31; Screw rod coupling shaft 31 and hot lid support 26 form revolute pair, and manual gear 29 and back-up ring 32 are all fixed with screw rod coupling shaft 31, and Hand-shifting wheel 30 is nested with on screw rod coupling shaft 31; Back-up ring pressing plate 33 compresses back-up ring 32, and is fixed by screw with Hand-shifting wheel 30; Manual tooth bar 39 is screwed on driver plate 35, and engages with manual gear 29.
The principle of work of this PCR quantitative fluorescence analysis instrument heat lid:
Motor 5 drives follower gear 7 to rotate by transmitting gear 6, and follower gear 7 engages with driven tooth bar 38 and drives driver plate 35 to travel forward, and travels forward together with union lever 9 from making push rod 8.When driver plate 35 travels forward, hot lid support 26 also correspondingly travels forward simultaneously, and pedestal fairload 11 slides along the L shape slideway of left limit groove 2 and right limit groove 3; When pedestal fairload 11 enters the vertical slideway of left limit groove 2 and right limit groove 3, install bin 12 does descending motion, until stop in the front signal position that locating stop piece 37 arrives strongback 41.Now, the draught of spring 19 is definite value, and the pressure exported also is definite value, and push rod 8 is in 90 ° with the angle of union lever 9, and motor 5 pairs of union levers 9 do not produce locking force, thus greatly improves the work-ing life of motor 5.
Driver plate 35 moves backward, and push rod 8 moves backward, and pedestal fairload 11 slides along the vertical slideway of left limit groove 2 and right limit groove 3, install bin 12 upwards lifting; When after the vertical slideway that pedestal fairload 11 exits left limit groove 2 and right limit groove 3, push rod 8 moves backward together with union lever 9, until the rear signal location that locating stop piece 37 arrives strongback 41 stops.
When testing the test tube needing to place different heights, need the consistence ensureing pressure to prevent the volatilization of reagent.Therefore, when placing different heights test tube, we drive screw rod coupling shaft 31 and screw rod 24 to rotate by rotating back-up ring 32, thus pressure regulator panel 20 is moved up and down, and the draught of regulating spring 19, makes it reach required force value.
When testing unexpected power-off, when needing manual unlocking swivel nut 25 to take away test tube, rotation manual thumb wheel 30 drives manual gear 29 to rotate, and driver plate 35 is moved backward, pedestal fairload 11 exits the vertical slideway of left limit groove 2 and right limit groove 3, and then with hand to the hot lid support 26 of pusher.
Claims (5)
1. a PCR quantitative fluorescence analysis instrument heat lid, comprise installation base plate, left limit groove, right limit groove, motor, transmitting gear, follower gear, push rod, union lever, pedestal fairload, install bin, aluminium sheet, spring, pressure regulator panel, caging bolt, screw rod, swivel nut, hot lid support, manual gear, Hand-shifting wheel, screw rod coupling shaft, driver plate, locating stop piece, driven tooth bar, manual tooth bar, line slide rail and strongback, it is characterized in that:
Described left limit groove, right limit groove, motor mounting rack and strongback are all fixed by screws on installation base plate, the fixed block of two line slide rails is fixed on installation base plate by screw symmetry, and two pieces of sliding shoes of each line slide rail are all fixed with slide block by screw and install rebound; Described driver plate is installed rebound by the symmetrically arranged two pieces of slide blocks of two blocks of side plates and two ends and is fixed, and hot lid support is installed rebound by two pieces of bracing frames and other two pieces of slide blocks and fixed; One end of described locating stop piece and one block of side plate are fixed by screw, and the other end is arranged on above strongback; Described motor is fixed on motor mounting rack; Transmitting gear is fixed on the output shaft of motor, and follower gear is hinged on motor mounting rack, and driven tooth bar is fixed on driver plate, and follower gear engages with transmitting gear and driven tooth bar simultaneously; Two push rods are symmetrical arranged, and one end is all fixed by screws on driver plate, and the other end is hinged with one end of a union lever respectively; Side walls hinged all with install bin of the other end of two union levers; The two ends symmetry of described install bin is hinged with two pedestal fairloads, and two pedestal fairloads form rolling pair with left limit groove, right limit groove respectively; Aluminium sheet and pressure regulator panel are all arranged in install bin, and heating film, heat insulating mattress and insulation plate are successively set on aluminium sheet, and are fixed by multiple securing bolt of equidistant arrangement and aluminium sheet; Be equipped with spring between all securing bolts and pressure regulator panel, pressure regulator panel compresses the inwall of install bin; Each spring is nested with on a caging bolt, and all caging bolts all through install bin, pressure regulator panel and insulation plate, and are threaded with aluminium sheet; Described swivel nut and pressure regulator panel are fixed, and one end that bottom and the swivel nut of screw rod stretch out outside install bin is connected by screw thread pair; Pressing plate is fixed by screws in outside install bin, and the shaft shoulder of clamping screw; The top of screw rod embeds in the square groove bottom screw rod coupling shaft; Described screw rod coupling shaft and hot lid support form revolute pair, and manual gear and Hand-shifting wheel are all fixed with screw rod coupling shaft; Described manual tooth bar is fixed on driver plate, and engages with manual gear.
2. a kind of PCR quantitative fluorescence analysis instrument heat lid according to claim 1, is characterized in that: described left limit groove and the slideway of right limit groove all L-shaped, comprise the vertical slideway of bottom and the horizontal slide at top.
3. a kind of PCR quantitative fluorescence analysis instrument heat lid according to claim 1, is characterized in that: be fixed with sealing-ring bottom described install bin.
4. a kind of PCR quantitative fluorescence analysis instrument heat lid according to claim 1, it is characterized in that: described pressure regulator panel is fixed with symmetrically arranged two the first spacing guide pillars and two the second spacing guide pillars, the top of two the second spacing guide pillars embeds in the stopper slot of the correspondence limited block be fixed on hot lid support respectively.
5. a kind of PCR quantitative fluorescence analysis instrument heat lid according to claim 1, it is characterized in that: described screw rod coupling shaft is fixed with back-up ring, Hand-shifting wheel is nested with on screw rod coupling shaft; Back-up ring pressing plate compresses back-up ring, and is fixed by screw with Hand-shifting wheel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510094330.6A CN104673667B (en) | 2015-03-03 | 2015-03-03 | A kind of PCR quantitative fluorescence analysis instrument heat lid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510094330.6A CN104673667B (en) | 2015-03-03 | 2015-03-03 | A kind of PCR quantitative fluorescence analysis instrument heat lid |
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| CN104673667A true CN104673667A (en) | 2015-06-03 |
| CN104673667B CN104673667B (en) | 2016-09-07 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132283A (en) * | 2015-08-21 | 2015-12-09 | 杭州安杰思生物科技有限公司 | Hot lid mechanism of fluorescent quantitative PCR (polymerase chain reaction) instrument |
| CN105969638A (en) * | 2016-06-23 | 2016-09-28 | 浙江爱易生物医学科技有限公司 | Adjustable fluorogenic quantitative PCR instrument |
| WO2022001990A1 (en) * | 2020-06-30 | 2022-01-06 | 利多(香港)有限公司 | Internal fixing device for apparatus in transport mode, apparatus comprising internal fixing device, and method for reducing damage to apparatus during transport |
| CN116699403A (en) * | 2023-05-12 | 2023-09-05 | 江苏风驰碳基新材料研究院有限公司 | Automatic detection device for sodium ion battery |
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| EP2163172A1 (en) * | 2008-09-10 | 2010-03-17 | Man Wah Furniture Manufacturing (Huizhou) Co., Ltd | Drive for adjusting parts of seating and reclining furniture |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132283A (en) * | 2015-08-21 | 2015-12-09 | 杭州安杰思生物科技有限公司 | Hot lid mechanism of fluorescent quantitative PCR (polymerase chain reaction) instrument |
| CN105969638A (en) * | 2016-06-23 | 2016-09-28 | 浙江爱易生物医学科技有限公司 | Adjustable fluorogenic quantitative PCR instrument |
| CN105969638B (en) * | 2016-06-23 | 2018-11-30 | 浙江爱易生物医学科技有限公司 | A kind of adjustable fluorescence quantitative PCR instrument |
| WO2022001990A1 (en) * | 2020-06-30 | 2022-01-06 | 利多(香港)有限公司 | Internal fixing device for apparatus in transport mode, apparatus comprising internal fixing device, and method for reducing damage to apparatus during transport |
| CN116699403A (en) * | 2023-05-12 | 2023-09-05 | 江苏风驰碳基新材料研究院有限公司 | Automatic detection device for sodium ion battery |
| CN116699403B (en) * | 2023-05-12 | 2024-01-05 | 江苏风驰碳基新材料研究院有限公司 | Automatic detection device for sodium ion battery |
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| CN104673667B (en) | 2016-09-07 |
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Address after: Hangzhou City, Zhejiang province 310053 high tech Industrial Development Zone Waterfront Road 1192 Patentee after: Hangzhou bori Technology Co., Ltd Address before: Hangzhou City, Zhejiang province 310053 high tech Industrial Development Zone Waterfront Road 1192 Patentee before: HANGZHOU BIOER TECHNOLOGY Co.,Ltd. |